LOCUS       NZ_CP130920             9293 bp    DNA     circular CON 14-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 2023CK-00047 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP130920
VERSION     NZ_CP130920.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32978013
            Assembly: GCF_028364475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2023CK-00047
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 9293)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 14.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/11/2023 16:40:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,622
            CDSs (total)                      :: 5,496
            Genes (coding)                    :: 5,351
            CDSs (with protein)               :: 5,351
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 53 of 145
            Pseudo Genes (incomplete)         :: 94 of 145
            Pseudo Genes (internal stop)      :: 21 of 145
            Pseudo Genes (multiple problems)  :: 20 of 145
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130920.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CK-00047"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2022-12-21"
     gene            1..150
                     /locus_tag="Q6H54_RS28060"
                     /old_locus_tag="Q6H54_28060"
     CDS             1..150
                     /locus_tag="Q6H54_RS28060"
                     /old_locus_tag="Q6H54_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1630..3594
                     /locus_tag="Q6H54_RS28065"
                     /old_locus_tag="Q6H54_28065"
     CDS             1630..3594
                     /locus_tag="Q6H54_RS28065"
                     /old_locus_tag="Q6H54_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3594..4325
                     /locus_tag="Q6H54_RS28070"
                     /old_locus_tag="Q6H54_28070"
     CDS             3594..4325
                     /locus_tag="Q6H54_RS28070"
                     /old_locus_tag="Q6H54_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4332..4862
                     /locus_tag="Q6H54_RS28075"
                     /old_locus_tag="Q6H54_28075"
     CDS             4332..4862
                     /locus_tag="Q6H54_RS28075"
                     /old_locus_tag="Q6H54_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4890..5069)
                     /locus_tag="Q6H54_RS28080"
                     /old_locus_tag="Q6H54_28080"
     CDS             complement(4890..5069)
                     /locus_tag="Q6H54_RS28080"
                     /old_locus_tag="Q6H54_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5138..5515
                     /locus_tag="Q6H54_RS28085"
                     /old_locus_tag="Q6H54_28085"
     CDS             5138..5515
                     /locus_tag="Q6H54_RS28085"
                     /old_locus_tag="Q6H54_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5577..5876)
                     /locus_tag="Q6H54_RS28090"
                     /old_locus_tag="Q6H54_28090"
     CDS             complement(5577..5876)
                     /locus_tag="Q6H54_RS28090"
                     /old_locus_tag="Q6H54_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5866..6129)
                     /locus_tag="Q6H54_RS28095"
                     /old_locus_tag="Q6H54_28095"
     CDS             complement(5866..6129)
                     /locus_tag="Q6H54_RS28095"
                     /old_locus_tag="Q6H54_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6435..6854)
                     /locus_tag="Q6H54_RS28100"
                     /old_locus_tag="Q6H54_28100"
     CDS             complement(6435..6854)
                     /locus_tag="Q6H54_RS28100"
                     /old_locus_tag="Q6H54_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7257..8942
                     /locus_tag="Q6H54_RS28105"
                     /old_locus_tag="Q6H54_28105"
     CDS             7257..8942
                     /locus_tag="Q6H54_RS28105"
                     /old_locus_tag="Q6H54_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8952..9209
                     /locus_tag="Q6H54_RS28110"
                     /old_locus_tag="Q6H54_28110"
     CDS             8952..9209
                     /locus_tag="Q6H54_RS28110"
                     /old_locus_tag="Q6H54_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     BGC             2257..9293
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(254..831)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.139"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3595..4322
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atgaaaaagg taaaggttgt ttttcttttt atattgattt tttcaggctt tcttctggtc
       61 gcatgtcagg caaactatat ccgggatgtt cagggtggaa cggtggcacc atcgtcctcc
      121 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
      181 tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga
      241 tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca
      301 gcaaccaaat ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc
      361 gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg
      421 ttttgcgtgc ttttccgggt tggactcaag acgatagtta ccggataagg cgcagcagtc
      481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      541 gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      601 caggtatccg gtaagcggca gggtcggaac aggagagcgc aagagggagc gattcatcgg
      661 aaacggtgat gatctttaag tcctgtcggg tttcgccact cctgactgat tcatggttga
      721 gccaccggct cccacagatg caccgaaaaa gcgtctgttt atgtgaactc agtcaggagg
      781 gcggagccta tggaaaaacg ccacctgcgt ggccttgccg ttttgcctca catgttagtc
      841 ccctgcttat tcacggaatc tgtgggtaac tttgcatgtg tccgcagcgc ccgccgcagt
      901 ctcacggccg gagcgtagcg accgagtgag cgaggaggcg caatttcccc ggcacctaca
      961 agacggacac cggacgcgct gatgtggcat attgtcccct gattaagtct ttatattccc
     1021 tgataataaa ttaccaggat aggtgaatca tatcggtttt tcgctgaaca ttttacgtta
     1081 acctatctct gttgcttttt tctccttacg gagaaataca ggtcatcccc gcgagggcgt
     1141 ggctgacctc agggggatat cccctgctta cgctggctgg acgccaccga cgcacggtct
     1201 ctccccctct ttctggctcc gtccggctgg acgccggcca cgccagaaat tcacccggcc
     1261 cccgtcaggc tcacgcctga ccacccacaa aaatccacgg tgctggacgc acctggcgaa
     1321 gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct cgctatggcg aactctgacg
     1381 ggggccggaa caccggaaaa gaccgcactg gggtgccgcc ttttcctctc acacgctggg
     1441 gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc
     1501 tccgtggtga aagctgaacg caatcaccac ggacgtgacc gccgccgcca tgccttgggg
     1561 catgtccgcg gcttgtgcag cgggggctgc acatcacgcc ggcaaaccat cgtctgaagg
     1621 agagagaaca tgtttaatac ggattcgctt gcctggaagt ggcacattcc ggagctggcc
     1681 cggcagattt atccgtgggt cagctggctg taccaggagt attacggtct ggccggggct
     1741 ttcctagtta tcggcctgtt tttcggtctg gctgaacttt gggccggacg gatgcgtttt
     1801 gtggggctga gtcataagga gcgacgggag tatgccgatc gctataagcg cgataataaa
     1861 cggccagcgc cattttcggt gctggcctcg ctggattcgc ccgggcggat ggtcagggca
     1921 cgtcgacatg tctggaacta cgcgtactgg ctgaccggcg cggccggtct ggcccttctg
     1981 ggggggctgg ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgtccggac
     2041 ctctacgcaa acgcagatta ttacctgctg cagccggcta acctgttcgg gattgcctgg
     2101 tgcgcgggcg ggctggctgt cgggattgtg ttggcgcggc tgctggcgtt cctgctgctt
     2161 gacggtcatt ttgcggctgc tgacgaggcg gtgaacgcga aactgaatca ggagagccgg
     2221 cgcagcagcc agcggacagg agagatgacc gacgtgcgcc acctgcattt cggggagccg
     2281 gtaccggtta atgcgctggc cgatttttcc tcggagcagg cccggcagga gcaggcggtg
     2341 tttctgggta aggatgagca gggcaagccg gtgctggttc cgcgtgatac ctggcgcaag
     2401 accaacattc agatcctcgg cctgcccggg agcggcaaga gcgtgatggc caccaacgcg
     2461 ctcattcgct gcgtacgcga tttcggggac gcggtggtgt acttcgaccc caaaggggac
     2521 gcatgggcgc cgcacgtctt ccgggcgcac tgtccggact tcaccctgct ggatctgcgg
     2581 ccggggaaac cggcgcagct gaatctgttc cgcgatctcg accagtacgc gctgaaaaac
     2641 ctgctgattg ccgggcttaa cctgggcgag accggcgacg tggcggacca ctaccgcatc
     2701 agcgagcaga aagcggcgaa gctgattgcg gagcagtttc cgcagggggc aaacattcag
     2761 caggtgctgg cggcggcgta cgcgctgccg gaggccctga aaaaggacgt gaaggggttg
     2821 atcactaggc tggaaaacgt cgctgacctg agcgtcctgc agacggacag tggcatcgat
     2881 gtggcgggga ttatcaacgg cggcggctgc ctgtacgtta tcggctcgat ggacgacgag
     2941 gcggtgatcc gtgtgcaaaa aatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg
     3001 gacgagttcc gggagtggcc acacgtcagc gtgatgctcg atgaaattaa atatctgctg
     3061 tcgaagtatg tactgaacgc actggggacg ctgcgcagcc gggactgcaa tctgttgctc
     3121 gcgcaccagt cgctgggcga tttcgggcag tgcgggcagg atctgcccgc tgaatttgtg
     3181 aaaaccacgg tactggataa cacgccgatc cgctggtttt accgggcggc cagcctggag
     3241 tccgcgcagt gggcggcggg gcagaccggg gaaatcaggg tggacgtgga gcgccgccgt
     3301 gccagccggg aggcggggaa cgtggagcat atcagcgggg acagcttcat ccagaaggag
     3361 gcgcggccgc tgtttgacgt gaacaccctg caacacctgc cggacgggtt cgcggtcatg
     3421 accgggctgg gtgtggcccg gatggcgttc agcagtccgc tgcgggttga gcgccgggaa
     3481 atcccgttgc agcgtttccc ggtgctggcg aaaaccgatc ccctggcgga gttccggccc
     3541 ccggccgacg gcggcgggcc gaaagcggat gatgattttg cggggattta ctgatggcgc
     3601 tggagctgta caacgtcagc cacgcgaaac gacaggcgcg gaacgcggag aaaacccggc
     3661 tgaccctgcg ctggttgcgg gaagagctgt gttccacggc ggagctggtt gcgcggcggc
     3721 tggggattgc ggcggtgcag ccggtctacc gcttcctcga cagcctggtg gcgaaagggc
     3781 tgctggtccg gaccaaatat ccggtggacg gccgtcaggt cagcgtgtgg gggctgacgc
     3841 cgcatggcgt ggcgttctcg ttcgacgagg acgaaccgct ggcggatgtt attccgtttc
     3901 agccgtcaag ggtgtcggca gcacatctgc cgcaccggct ggccgtgcaa tccctgcgcc
     3961 ttgatatgga agccagaggg gccaccggct ggcgcattct gcaccgtctg agtcttaaag
     4021 gcatgaaagt gccggacgcc ctggcggagc tggacggtaa aacggtggcg tttgaggtgg
     4081 agcgcaccgt caagagccgt cgccggtatc aggaagtggt gtccgggtat ctgttcaacc
     4141 gcaaagctaa cggcatcgat gaaatctggt acatctgccc ggaccgggcg acgcaggtca
     4201 gggtgcagcg ggcaatcctg tcggtggatg aaatcgtgaa cccacagacc ggcgaggccc
     4261 gcaaaacggc agagctggat cgtgaacggt tgtttgcgtg ctttaaattt atgacaacgg
     4321 agtgagggat tatggcgctg tacatggtat ttattgaaga cgcggaggac cggaacggcg
     4381 agcgctgcat catgccgacc agcgagcatc tggaagatat ggtggtgctc gcctggcaga
     4441 ccggggcgta tgttttctgt tttgtcgacc gggcccagca tctggcgatg atgaaatcca
     4501 tcgggatgta tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt ttcactggta
     4561 aaaatccgca atgggaacgg aacaaaccca caggaatgaa gctgtgtgat tacctctatc
     4621 gcaaggtgga gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct
     4681 caccggagca gattaacgcg atgattgacg tggtgagtga gcggcacggg gaaagcgcgg
     4741 tgaacgagaa gctgaaagcg gagctgatgc gggaatataa cagccgctat tttaacgtgc
     4801 cggtacctga actgccggag gacaaacctg cccgccggtg gtggccgttc ggcaaaaaat
     4861 aaatgccagc accggccatg ccggtgctgt tattcctccc cgagcttatc ccgcatcacc
     4921 tgccagagtg tttccgcatc ctcgtgtagc cgctcgcaca tgtcggccgg ggtatccata
     4981 tccagctcat tcagtttatc gagcaatagc agcgactgct ccttaataaa tttagccata
     5041 ttgagggctg ttttttcctg ttttgtcatg attctttcct tccgggcatg gtgtgcgcct
     5101 gtcaggtata ctgtctcaaa aatagcgggg tgagacaatg gcgtgggtga cggtgagaca
     5161 ggcgacggaa ctgacgggta aagcccgaag ttccctgtat cgggatatgg cgaaaggccg
     5221 cgtatcatac cgtacggaag ctgatggcgg ccgggtggtg gataccagcg agcttatccg
     5281 ggtgtacggt gagttaagac aggatgagac tcacgagcgg gacgagttga gactgtcggg
     5341 tgagactgaa aaaatggcct ccgacgcgtt gatcgcggag ataaaagcac tcagggagga
     5401 ggtggccggg ctacggcagg aaatgcagtc catgagactg ctggaacaca aaccggagac
     5461 agcagagacc ccggccggaa caccgcgccg gtggtggccg tggggaaaac gctgacgcgc
     5521 tgtgcttgtc caaccggcga cggccacgca atgaattgcg tccccgtcac cggttattag
     5581 agaacaatat ggggattctg gcgctggccg accacggcca gaacccggac ttttctggtc
     5641 tggtcatcaa catcatagag gacatgctgt cccatcagac tgatccggcg aacgtcctcg
     5701 ccatagcgtg agtccacttc atagagtcgc gggaagtcgg ccagtttgtc cgccgcagcg
     5761 agaacccggt cggtaacgtt ctgcgcatcc cacagggatg aattttccgc gatatagtca
     5821 aacagcgtcg ttacatcctc gatggctccc tgcttccatt ccacgtcata cggcatcgcg
     5881 cagttcctga taacggccat gcaggcgctg gcggagttgt tccgtggtca tacccggacg
     5941 cgtgtcagca cgggcggcgg tcactttggc gcgaacctga tcttcatact ctgcctgggc
     6001 cagttcttcg ttagctagtc gctgcaacag cgcagctttg tccgggccac cttccggcag
     6061 tatgcggggc aggctttcag ccagttcccg aagaaagttg gcgttttcgt agtgtgtagt
     6121 gttgctcatt gcggagcctc ccgggttaat gcggcaatat gaaggaagtt tagcaggggg
     6181 cggtctgaaa gaatatggtc gtgtcagtca ggctgatgaa gcagaaagtc ggtaaaaaag
     6241 ggcttacggc atccatttta cgtcaaaaca tatgccatgc agttgcgtgc ataggcatgc
     6301 attaggataa aatttatcgg gcgcgtttcc ggcagttttc cgggggggtt gttgcctgtt
     6361 ttatcccgta gccgccggaa acgccctgag cccgtctgag cggtgcgcgt aatgacgcgt
     6421 tatggtaaat ccacttactg tctgccttca tagccatcga gataaacagc tgaaatggtg
     6481 tcagccagta gccttgattt tttcggtgtg aggtctttgc tgcggtaatc ggtacccgca
     6541 aattgtgttg tgccttggag tgagtttaaa aattcctgac tgtgaaactg ctgaatgcgt
     6601 tgctgcacat cactccgtga ttttccggtc gctttatcct ttgcgcccat atcccagaac
     6661 tgccggaaaa aaggcaccat cattcgtgtt gtgtcagctt tctgggtgga aaaattggga
     6721 tcaaaaccgt catctgaagc atatacgtaa tgccgggtat gctgttccac gctgcccttt
     6781 ggagcacagg cagtgagagt tgctgtcgct gttatcatgg cgataagaca cagtttgctg
     6841 ccggaacgtc tcatgagcgt ctccttacct ttaattcttt tttctcattg agttatatat
     6901 ccgcaggcta tcttatcatc agtttctgac cgtgttcagt gaccgaacaa cccgaaccag
     6961 accccccgtt tcttttcctg tcccggaggg ggctttctgg tatctcatat aatgatttca
     7021 gtaaaacata ctgaggccgg agaacggcgc cgtttgtgct ggtttttgtt gcatctggag
     7081 gggattgcgc atggtgtgag tggctgtttt tttgatcatt ttcacaaaaa cgcaaattta
     7141 cagacttgac atgaaaaaca aggcagcgta atttttatac tgtatataaa accagtggtt
     7201 atatgtacag tattttattt tagtttattg ttttaaaagt caaagaggaa aagtatatga
     7261 gtggcggaga cggacgaggt ccgggtaatt caggtctggg acacaatggt ggtcaggcca
     7321 gtgggaatgt gaacggtaca tcaggtaaag gtggcccttc atcaggtggt ggtacggatc
     7381 caaacagcgg gccgggctgg ggtacgacgc atacacctaa cggggatatt cataactata
     7441 acccggggga gtttggtaac ggcgggagta aacccggtgg taatggcggt aacagcggca
     7501 atcatagcgg gagctctggt ggcggacagt cttcggccac cgcgatggcc ttcggtctgc
     7561 ctgccctggc cactccgggg gctgaaggac tggctttatc cgtttccggc gatgcgttgt
     7621 cggccgctgt tgctgatgtg ctggctgccc tgaaaggtcc gtttaagttt ggtctgtggg
     7681 ggattgcgat ttacggagtg ctgccttctg agatagcaaa agatgatccg aaaatgatgt
     7741 caaaaatcgt gacgtcattg ccggcagaca cggtaacgga gacgccggtc agctccctgc
     7801 cgctggacca ggcgacggtc agcgtcacta aacgtgtggc ggatattgtg aaggacgagc
     7861 ggcagcatat tgcggttgtc accggccggc caatgagtgt tcctgtggtg gatgcgaaac
     7921 cgacaaaacg tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga
     7981 gcgtacctaa aggtgttccg gccgcgaaag ccccgccaaa aggcattatt gctgaaaaag
     8041 gtgattcacg tccggctggt tttacggccg gtggtaactc ccgtgaggcc gttattcgtt
     8101 tcccgaaaga gagcggacag aaaccggtct atgtgtcggt gacggatgtt cttactccgg
     8161 cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg gacgctgctc
     8221 acccggaaga ggggctgaaa agagagtatg ataaagcgaa agctgagctg gatgctgaag
     8281 ataaaaatat tgcgacctta aacagtcgca ttgcatcgac agagaaggcg atccccggtg
     8341 caagggctgc tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg
     8401 attttgtgac ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct
     8461 atcttgataa ggatattcag aatcagaatg agaaattaaa agctgcccag acatctttaa
     8521 acgaaatgaa tgaatcctta tccagggata aggctgcgct ttccggggcg atggagagcc
     8581 ggaaacaaaa ggagaaaaaa gcgaaggatg cagaaaataa attaaatgag gaaaagaaaa
     8641 aacctcgtaa gggaactaaa gattacggcc atgattattt tcctgatccc aagactgaag
     8701 atattaaggg gttgggagag ttgaaagagg gtaaacctaa aacccctaaa caaggtggtg
     8761 gtggtaagcg ggcccgatgg tatggtgata aaaagcgtaa aatttatgaa tgggattccc
     8821 agcacggtga gcttgaaggg taccgcgcca gtgatggcga acacctcggg gcattcgatc
     8881 caaaaacggg taagcaggtt aaagggccgg atccaaaacg aaacattaaa aaatatcttt
     8941 aagaggtaaa tatggggctt aaattacata ttcattggtt tgataagaaa accgaagagt
     9001 ttaaaggtgg tgaatactca aaagacttcg gtgatgatgg ttctgtcatt gaaagtctgg
     9061 ggatgccttt aaaggataat attaataatg gttggtttga tgttgaaaaa tcatgggttt
     9121 cgatattaca gccacacttt aaaaatgtaa tcgatattag taaatttgat tactttgtat
     9181 cctttgttta ccgggatggt aactggtaag caaaagttat caaggatgat ttgaaataca
     9241 gacttccggt gttcagggat gaataccggg gtttttaaat acagggaagg tat
//
