LOCUS       NZ_CP130691            11970 bp    DNA     circular CON 01-AUG-2023
DEFINITION  Klebsiella pneumoniae strain F1025 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP130691
VERSION     NZ_CP130691.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36465167
            Assembly: GCF_030552995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Shen,W. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2023) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine,
            Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v.2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Oxford Nanopore
            PromethION 48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 16:05:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,945
            CDSs (total)                      :: 5,822
            Genes (coding)                    :: 5,641
            CDSs (with protein)               :: 5,641
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 71 of 181
            Pseudo Genes (incomplete)         :: 117 of 181
            Pseudo Genes (internal stop)      :: 21 of 181
            Pseudo Genes (multiple problems)  :: 28 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130691.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F1025"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China"
                     /collection_date="2023"
     gene            join(10897..11970,1..891)
                     /locus_tag="Q5M90_RS29660"
                     /old_locus_tag="Q5M90_29660"
     CDS             join(10897..11970,1..891)
                     /locus_tag="Q5M90_RS29660"
                     /old_locus_tag="Q5M90_29660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            891..1622
                     /gene="mobC"
                     /locus_tag="Q5M90_RS29665"
                     /old_locus_tag="Q5M90_29665"
     CDS             891..1622
                     /gene="mobC"
                     /locus_tag="Q5M90_RS29665"
                     /old_locus_tag="Q5M90_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1629..2159
                     /locus_tag="Q5M90_RS29670"
                     /old_locus_tag="Q5M90_29670"
     CDS             1629..2159
                     /locus_tag="Q5M90_RS29670"
                     /old_locus_tag="Q5M90_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2186..2365)
                     /locus_tag="Q5M90_RS29675"
                     /old_locus_tag="Q5M90_29675"
     CDS             complement(2186..2365)
                     /locus_tag="Q5M90_RS29675"
                     /old_locus_tag="Q5M90_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2391..2819)
                     /locus_tag="Q5M90_RS29680"
                     /old_locus_tag="Q5M90_29680"
     CDS             complement(2391..2819)
                     /locus_tag="Q5M90_RS29680"
                     /old_locus_tag="Q5M90_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2983..3297)
                     /locus_tag="Q5M90_RS29685"
                     /old_locus_tag="Q5M90_29685"
     CDS             complement(2983..3297)
                     /locus_tag="Q5M90_RS29685"
                     /old_locus_tag="Q5M90_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3285..3563)
                     /locus_tag="Q5M90_RS29690"
                     /old_locus_tag="Q5M90_29690"
     CDS             complement(3285..3563)
                     /locus_tag="Q5M90_RS29690"
                     /old_locus_tag="Q5M90_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3738..4103)
                     /locus_tag="Q5M90_RS29695"
                     /old_locus_tag="Q5M90_29695"
     CDS             complement(3738..4103)
                     /locus_tag="Q5M90_RS29695"
                     /old_locus_tag="Q5M90_29695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4100..4471)
                     /gene="tolA"
                     /locus_tag="Q5M90_RS29700"
                     /old_locus_tag="Q5M90_29700"
     CDS             complement(4100..4471)
                     /gene="tolA"
                     /locus_tag="Q5M90_RS29700"
                     /old_locus_tag="Q5M90_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4745..4990)
                     /locus_tag="Q5M90_RS29705"
                     /old_locus_tag="Q5M90_29705"
     CDS             complement(4745..4990)
                     /locus_tag="Q5M90_RS29705"
                     /old_locus_tag="Q5M90_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5635..7122
                     /gene="ltrA"
                     /locus_tag="Q5M90_RS29710"
                     /old_locus_tag="Q5M90_29710"
     CDS             5635..7122
                     /gene="ltrA"
                     /locus_tag="Q5M90_RS29710"
                     /old_locus_tag="Q5M90_29710"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7227..8912
                     /locus_tag="Q5M90_RS29715"
                     /old_locus_tag="Q5M90_29715"
     CDS             7227..8912
                     /locus_tag="Q5M90_RS29715"
                     /old_locus_tag="Q5M90_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8922..9179
                     /locus_tag="Q5M90_RS29720"
                     /old_locus_tag="Q5M90_29720"
     CDS             8922..9179
                     /locus_tag="Q5M90_RS29720"
                     /old_locus_tag="Q5M90_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9264..9413
                     /locus_tag="Q5M90_RS29725"
                     /old_locus_tag="Q5M90_29725"
     CDS             9264..9413
                     /locus_tag="Q5M90_RS29725"
                     /old_locus_tag="Q5M90_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2227..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9517..10095)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          892..1619
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gagcagaaag cggcgaagct gattgcggag cagttcccgc agggggcaaa cattcagcag
       61 gtgctggcgg cggcgtacgc gttgccggag gccctgaaaa aggacgtgaa ggggctgatc
      121 accaagctgg agaacgtggc cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg
      181 gcggggataa tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg
      241 gtgatccggg tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat
      301 gagttccgga ggtggccgca cgcgagcatc atgctcgatg agattaaata tctgctgtcg
      361 aagtacgtgc tgaacgcgct ggggacgctg cgcagccggg actgcaacct gctgctcgcg
      421 caccagtcgc tgggcgactt cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa
      481 accacggtac tggataacac gccgatccgc tggttctatc gcgcggccag ccaggagtcc
      541 gcgcagtggg cggcgggcca gaccggggag atccgggtgg acgtggagcg ccgccgggcc
      601 agccgggagg cggggaacgt ggagcacatc agcggcgaca gcttcatcca gaaggaggcg
      661 cggccgctgt ttgacgtgaa caccctgcaa cacctgcctg acgggttcgc ggtgatgacc
      721 gggctgggcg tggcgcggct ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt
      781 tcgctgaaaa ccttcccggt gctggcgaaa acggacccgc tggcggagta cctgcctccg
      841 gccgacggcg gcggaccaaa agcggatgat gattttgcgg ggctttactg atgacactgg
      901 agcagtacaa cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acacggctga
      961 ccctgcgctg gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg
     1021 ggattgcggc ggtacagccg gtttaccgct tcctggacag tctggtggcg aaagggctgc
     1081 tggtccgggc gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc
     1141 acggggtggc gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc
     1201 cgtcaagggt gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgcctct
     1261 ccatggaggg ccggggggcc accggctggc ggtacctgca ccgtatggcc ctgaaaggga
     1321 tgaaagtgcc ggacgcgctg gcggagctgg acggcagaac ggtggcattt gaggtagagc
     1381 gcaccgtcaa gagccgcagg cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc
     1441 gggcgaacgg catcgatgaa atctggtaca tctgccctga ccggccgacg cttgtccggg
     1501 tacagcgggc gattctggcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca
     1561 aaacggtgga gctggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacggagt
     1621 gagggattat ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc
     1681 gctgcattat gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg
     1741 gggcgtatgt tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg
     1801 ggatgtatga cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa
     1861 atccgcaatg ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa
     1921 aggtggagcg gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac
     1981 cggagcagat taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga
     2041 acgagaagct gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg
     2101 tgcctgaact gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac
     2161 gccagcaccg gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc
     2221 agagtgtttc cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca
     2281 gctcgttcag cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga
     2341 gggcagtttt ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac
     2401 cagccggacc agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg
     2461 gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta
     2521 tcttccagca tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga
     2581 agctgttcct gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt
     2641 ttctcaggga tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg
     2701 ttgtttttcc cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt
     2761 cttctggtaa tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact
     2821 gtatacccct gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc
     2881 tgaattttac ccgtttttcc gttcaggatg tggttttctg acgcgctgcg cttgtcaaac
     2941 cggcgacggc cacgcaatga attgcgcccc cgtcatcggt tattagaggc tgtctgcccc
     3001 cggatacccg gattctttct gatgcctgac ggtgacgatc acaatctggt ccatttcccg
     3061 gtcgtggcgg tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata
     3121 ttccagcggc agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat
     3181 agcccggacg atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa
     3241 gtcctgaagg cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc
     3301 ggagcatcct gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca
     3361 gtaaggtgca gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac
     3421 tggctgcgct tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc
     3481 gctgagcggt gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc
     3541 ctgatggatg ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact
     3601 actttaatcc gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc
     3661 ttcgcttcca ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct
     3721 gcgggttaaa ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact
     3781 ggtcgcggat cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc
     3841 attgccgggt gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa
     3901 aaaaggtgac agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac
     3961 gtggcggtca cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc
     4021 agtgtggtta cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc
     4081 actgtcagag taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc
     4141 aggtattttc atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt
     4201 tacagagttt cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta
     4261 tggatatccg cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt
     4321 caacagcggt cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat
     4381 aaattttcgg cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca
     4441 atgccagcag gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt
     4501 tacatgtata tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta
     4561 tcgtttttac accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca
     4621 atttaccggg cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag
     4681 ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata
     4741 ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct
     4801 cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc
     4861 tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg
     4921 ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca
     4981 ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct
     5041 ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa
     5101 tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt
     5161 acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca
     5221 cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt gtgcgccggg
     5281 agaccggtta gagatcaagg ggtgaaagtc cccgaccatt gaaggaccag caatccacaa
     5341 ggtccccgag tcatgcgttg cataccgcga ggtatggggc gaagcgttga caggggtgtt
     5401 gacaggccag ccattgagcc acgaaatgta tattaaatta ccgggtgccg acgttgtact
     5461 gttaacggaa ggcaacatca tagggtgcga cactgcgagt gccacatgga cccggcgggg
     5521 tctgagaccc tggcatgtca atacgatctc tacgcgggaa ccgggagatc tcccctctga
     5581 ccatctgcca gtgtcggaga tggcccgcac cgggaagacg aggagtcata gccggtgatg
     5641 tacggagagg agaagtcgga ctcgctcata gtagcggcga agcaggcgaa caacccgaaa
     5701 ggagcggagt cagtggagcg aaggagcggg gccaagggga acgcggaaca gccacacatg
     5761 cgccggacac agagccggga aagcatgtca cagaggctgt cacgcgtgcg ggaagctgcg
     5821 aagcagcgga agaaagaacg gtttacagca ttgttccacc tgctgacagt cgaagcactg
     5881 gaagccgcat tcctctccct gagcaggaaa gcggccgccg gagtggatgg catcaggtgg
     5941 atggactacg ccggaaacat gaagaacaac ataacagatc tgcaccggag gctacatcag
     6001 ggcagctaca gggcgcagcc cggcaggcgt cactacatcc caaaagcgga tggaaaacaa
     6061 cgcccgctcg gcatcgcctc gctggaggac aagatcgtcc agtatgcgct ggtgaaaatc
     6121 ctgaacgcag tctatgaaaa cgactttatg gggttctcat acgggttcag acccgggcga
     6181 agccagcacg atgcactgga cgcactggcc acagggctgg tacgcactaa cgtaaactgg
     6241 gtactggatg ccgacatcag tcagttcttc gacagggtga gccacgaatg gctgatcagg
     6301 ttcacagagc atcggatcgg cgaccggagg gtaatcaggc tcatacgtaa gtggctcaca
     6361 gccgggacgt cggaggaggg tcaatggcga gcaacggagg aaggcacccc acagggtgcg
     6421 gtcatctcac cgctgctggc aaacatatac ctccactacg tcttcgatct gtgggcgcat
     6481 cagtggcgac gtcgctatgc cacaggcaat gtggtaatgg tcagatacgc cgatgacatc
     6541 gtcatcgggt tcgacaaacg atacgatgcc cggcgcttcc gtatagccat gcagcgcaga
     6601 ctgagggagt tcggactcac ggttcacccg gagaaaaccc gtctgatgga gttcggccgc
     6661 ttcgctgccg aaaaccgtgc catcagggga aaaggcaaac cagaaacgtt caacttcctc
     6721 gggttcacgc acatcagcgg gaaagatcgc aacggcaggt tcatgctgat acgaaagacc
     6781 cgccgggatc ggatgacggc aactctgaaa gccatcaaag acggtctgcg aaggcgctgg
     6841 cattactcaa tccccgaaca gggaaaatgg ctcaggagag tggttcaggg atacctgaac
     6901 tatcactcgg taccgggcaa cttccccacc atgcagaagt tcaggacaca cgtaacaaac
     6961 ctctggcgcc gggcgctcag gcgcaggagc cagaaggatg atacgacctg gacgaaagca
     7021 aacaaactgg cagccgcatg gctaccaagg gttcgggttc ttcatccatg gcctgtggag
     7081 cggttcaccg ccagacaccc gaggcaggag cccggtgcgt aaatcgcgca cgccgggatc
     7141 tgtgcggggg gtatccggta acgggtatcc ctaccgcgac atgttggctt taatttattg
     7201 ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt ccgggtaatt
     7261 caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag
     7321 gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc
     7381 atacgccgaa tggagatatt cataactata atccggggga gtttggtaac ggcgggagta
     7441 aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt
     7501 cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac
     7561 tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc
     7621 tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg
     7681 agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg
     7741 cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac
     7801 aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc
     7861 caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt
     7921 cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag
     7981 ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg
     8041 gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt
     8101 atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa
     8161 agcgtcgcca gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg
     8221 ataaagcgaa agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca
     8281 ttgcatcgac agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa
     8341 aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat
     8401 atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg
     8461 agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata
     8521 aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
     8581 cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc
     8641 atgattatca tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag
     8701 gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata
     8761 aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca
     8821 gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag
     8881 atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt aaattacgtt
     8941 tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct aaagactttg
     9001 gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat attaataatg
     9061 gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt aaaaatcaaa
     9121 taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt aactggtaac
     9181 aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat gaatgccgga
     9241 gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt tttatattga
     9301 ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat gttcagggcg
     9361 gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat
     9421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa
     9481 aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg
     9541 gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg
     9601 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt
     9661 accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc aagtgaacag
     9721 ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca gcccagcttg
     9781 gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa agcgccacac
     9841 ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg aacaggagag
     9901 cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc
     9961 cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct
    10021 gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct
    10081 gccgttttgc ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta
    10141 tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt agcgaccgag tgagcgagga
    10201 ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg ggatattgtc
    10261 ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga accatgtcgg
    10321 tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
    10381 tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg cttacgctgg
    10441 ctggacgcca ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg ctggacgccg
    10501 gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc
    10561 acggtgctgg acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc
    10621 cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc
    10681 cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc
    10741 gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca ccacggacgt
    10801 gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg ctgcgcatca
    10861 cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc
    10921 tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac
    10981 cgggagtatt acagtctggc cggttttttt ctggttatcg gcctgttttt cggtctggct
    11041 gaactctggg ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg gcgggagtat
    11101 gccgatcgct ataagcgcga taataaccgg actgtgccat tttcggtgct ggcctcgctg
    11161 gattcacccg ggcggatggt cagggcacgg cgacatgtct ggaactacgc gtactggctg
    11221 accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc
    11281 tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg cagattatta cctgctgcag
    11341 ccggctaacc tgttcgggat agcctggtgt gccggagggc ttgtcgccgg cgtccttctg
    11401 gcgcggctgc tggcgttcct gctgcttgat ggtcattttg cggccgctga cgaggcggtg
    11461 aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga aatgacagac
    11521 gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccacg
    11581 gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg
    11641 ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc
    11701 ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt cggggacgct
    11761 gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc
    11821 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgttccgc
    11881 gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc
    11941 ggcgacgtgg cggaccacta ccgcatcagc
//
