LOCUS       NZ_CP130688             8600 bp    DNA     linear   CON 01-AUG-2023
DEFINITION  Klebsiella pneumoniae strain F1025 plasmid unnamed2.
ACCESSION   NZ_CP130688
VERSION     NZ_CP130688.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36465167
            Assembly: GCF_030552995.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Shen,W. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2023) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine,
            Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v.2.9.2
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq 6000; Oxford Nanopore
            PromethION 48
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 16:05:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,945
            CDSs (total)                      :: 5,822
            Genes (coding)                    :: 5,641
            CDSs (with protein)               :: 5,641
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 71 of 181
            Pseudo Genes (incomplete)         :: 117 of 181
            Pseudo Genes (internal stop)      :: 21 of 181
            Pseudo Genes (multiple problems)  :: 28 of 181
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130688.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="F1025"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China"
                     /collection_date="2023"
     gene            <1..400
                     /locus_tag="Q5M90_RS27710"
                     /old_locus_tag="Q5M90_27710"
     CDS             <1..400
                     /locus_tag="Q5M90_RS27710"
                     /old_locus_tag="Q5M90_27710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_304398251.1"
                     /translation="TSRHACLFTTSGSVVFLALSHSQIKESKMSRSPIPVFWYENPAHY
                     EEFQKILSDAYVLPFDYHDWRIRTDSMVERYENSGIQAVKVVASTYDFITWCQAHGRDI
                     STKSCNDYAVSESGLQILRDREFDWGDE"
                     /name="hypothetical protein"
     gene            complement(722..1159)
                     /locus_tag="Q5M90_RS27715"
                     /old_locus_tag="Q5M90_27715"
     CDS             complement(722..1159)
                     /locus_tag="Q5M90_RS27715"
                     /old_locus_tag="Q5M90_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_077257742.1"
                     /translation="MLGYWFDLDANMNFEERLLRALDEAGISQSELGRRVGVNSQTVSN
                     WCNTGNFPRKEKLELFPQALGKPLYWFFMTDEEESSLQAVTASKTVLNQKQLELLEVFD
                     QLPEVEQDRFIELARTRLEELDRFMAEFLSKRKIPPPFEKD"
                     /name="helix-turn-helix domain-containing protein"
     gene            1248..1472
                     /locus_tag="Q5M90_RS27720"
                     /old_locus_tag="Q5M90_27720"
     CDS             1248..1472
                     /locus_tag="Q5M90_RS27720"
                     /old_locus_tag="Q5M90_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="WP_071561166.1"
                     /translation="MQETVQKKIISLCGSQSELARRLGKNSQTVSVWFRTQVASTEVLN
                     ACRVLDWQVTPHELRPDLYPNPTDGLPKD"
                     /name="YdaS family helix-turn-helix protein"
     gene            1475..2029
                     /locus_tag="Q5M90_RS27725"
                     /old_locus_tag="Q5M90_27725"
     CDS             1475..2029
                     /locus_tag="Q5M90_RS27725"
                     /old_locus_tag="Q5M90_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014228885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="WP_046622349.1"
                     /translation="MQTISFKNHTSMLGMQQKTENQYSPRRRDSVKCRTIFAAVREWEA
                     TLPGRAQEHVAQLVAEQWEKQDGRGISVNKQNLYRYLKNESGSEKYTSYVMQLSGAIAD
                     AMPIEIARKHGLKRGLTESELVAQAIKECSEAHQAKLLGAPLQKLEREIREAAIALFNM
                     LPADAAGPLLASISAVAPQFF"
                     /name="toxin YdaT family protein"
     gene            2074..3093
                     /locus_tag="Q5M90_RS27730"
                     /old_locus_tag="Q5M90_27730"
     CDS             2074..3093
                     /locus_tag="Q5M90_RS27730"
                     /old_locus_tag="Q5M90_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="WP_072353967.1"
                     /translation="MSIDAMRWAKKVKTGKSSAKAVLTWMADMCGADLCAFPSIPALAE
                     ATELDKKTVQSSLQYLISIGLIEDTGERRGKTKQIPVYRLLGVEESVAEIEHTQKREHY
                     QKRDRLNIPENGVVTAEKAPENGTVSCTQNNQTIPFFPSNDPKNGIRNLPEEPKDITPT
                     HRVLVEPVVPDYPNQPGIVPGETQAFGKFAMYFGWKPSEDFPRLSTIWGMPLRPGINLA
                     AELSSFIAYWQVEGRAFHQVQWEQKLARHLNRAEVRQKKPANGSNDHVGVRAEPAASRA
                     VQQIRAAREQRLRVAGTDGRRNGVAPMGSDGRNLFEPMDPEERRGTIRALDRSDWVDE"
                     /name="DnaT-like ssDNA-binding domain-containing protein"
     gene            3005..3550
                     /locus_tag="Q5M90_RS27735"
                     /old_locus_tag="Q5M90_27735"
     CDS             3005..3550
                     /locus_tag="Q5M90_RS27735"
                     /old_locus_tag="Q5M90_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160640.1"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="WP_223172883.1"
                     /translation="MGEIFSNRWILKNGEEPSELWIAQIGSMSEAQITLVCRQCMERCA
                     AGSTWPPDLAEFVALVSASGANPFSLTSDAVMAEYKRWRNESYRYSGSDKYPWKQDVLY
                     HICIEMRRTGVERNLTEGELKKLAENLLTKWTKHMANGFSIPPIRRQLAAPRHPAGPTP
                     AQILMGEYKRRKAAGLTK"
                     /name="replication protein P"
     gene            3564..4004
                     /locus_tag="Q5M90_RS27740"
                     /old_locus_tag="Q5M90_27740"
     CDS             3564..4004
                     /locus_tag="Q5M90_RS27740"
                     /old_locus_tag="Q5M90_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032443841.1"
                     /translation="MTKQSKTKVTKAQMVLAIVSRTPECVLQDVCDALDLQASTAGNLL
                     RQLHAAGKLHRTHNGCQYVYRVGAGVEVPDVALPQAATQLSEEDVKKVQDALSLAKMLE
                     DKKLWRRAATVYTSMLGMATTANELWLLARMRNRCLRNVARW"
                     /name="hypothetical protein"
     gene            4418..5398
                     /locus_tag="Q5M90_RS27745"
                     /old_locus_tag="Q5M90_27745"
     CDS             4418..5398
                     /locus_tag="Q5M90_RS27745"
                     /old_locus_tag="Q5M90_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019445.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            complement(5499..5711)
                     /locus_tag="Q5M90_RS27750"
                     /old_locus_tag="Q5M90_27750"
     CDS             complement(5499..5711)
                     /locus_tag="Q5M90_RS27750"
                     /old_locus_tag="Q5M90_27750"
                     /inference="COORDINATES: protein motif:HMM:NF025612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="WP_227537839.1"
                     /translation="MRLYIIGNGFDIRHGLPTRYKHFKSYVAKNDKELYDAIEEYIPAG
                     DEWNELESALGKVRISRSFLPKLTR"
                     /name="AbiH family protein"
     gene            6063..6776
                     /locus_tag="Q5M90_RS27755"
                     /old_locus_tag="Q5M90_27755"
     CDS             6063..6776
                     /locus_tag="Q5M90_RS27755"
                     /old_locus_tag="Q5M90_27755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062954976.1"
                     /translation="MADYINSNILCESYVHVEPEWLKQVSAVIREEKLSLIRNEIEDFA
                     DKRIKFFLSDDVLIEIKFTEGSIKAKVTAYGPIILLIGSAILNYSSFRESVKQLASDMY
                     RASSMLSSEMLFQTNSRNKSEILRIEARKGIVGSIERINNKIDSINSQLDGKELSPTRI
                     NSELLDLNDMILTLLSNLKNAEDTKLVASSLSDGVSKLSIRKSRFKLKNKLDESMYASL
                     FNEKKSIIKNLAQYK"
                     /name="hypothetical protein"
     gene            6950..8311
                     /locus_tag="Q5M90_RS27760"
                     /old_locus_tag="Q5M90_27760"
     CDS             6950..8311
                     /locus_tag="Q5M90_RS27760"
                     /old_locus_tag="Q5M90_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198514.1"
                     /GO_function="GO:0008832 - dGTPase activity [Evidence IEA]"
                     /GO_process="GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage deoxyguanosine triphosphatase"
                     /protein_id="WP_062954975.1"
                     /translation="MTRSEINVWDVRRGTNSPRVEEIRSEAQRDLSRLIHSSPFRRLQS
                     KTQVLGLGESDFYRTRLTHSMEVAQIGSGLLAKLKNIYKGKEEAQFLPDNDLMQAVCLA
                     HDIGHPPFGHGGEVALNYCMLDYGGFEGNGQTLRILSKLDKYTLEHGLDPTRRLLLGIL
                     KYPVKYSELVVKKDSIPATIGSPNWLFKSSDFKPPKCYLDSECDIVEFALSPFTEHDRR
                     LFTSVKDGETKSDHKKASYKALDTTIMDLADEISYSLHDLEDAISLGMVTRAMWMEHFS
                     GHETLFTDCHGKTFSGTCESVADSLFGKSYKRKECIGMLVHLMITSVELNTKNAAFKCG
                     MLKYNAVLPEAVEALRLKIFKLVENKVIKHENVQQLEFKGQKLIVELFGVLANDPCRFL
                     PASTREIYEQQISLDEKAGMRVICDYVSGMTDGYATKLYEKILIPRKGSIFDHL"
                     /name="anti-phage deoxyguanosine triphosphatase"
ORIGIN
        1 tactagtcgt catgcctgtc ttttcaccac ttcaggctcg gtggtattct tggcgctctc
       61 acacagccaa ataaaagaga gcaaaatgtc tcgtagccct atacctgtct tctggtacga
      121 aaatcccgct cactatgaag aattccaaaa aatcctttca gatgcttacg tccttccctt
      181 tgactaccac gactggcgta tccgcaccga tagcatggtg gagcgctacg aaaacagcgg
      241 tatccaggct gtgaaggtgg tagccagcac ttacgatttc atcacctggt gccaggccca
      301 tggacgtgat atcagtacca aaagctgcaa tgattacgcg gtctccgaat cgggcctcca
      361 aatcctgcgc gacagagagt ttgattgggg agacgagtaa aaagtaaatt ttccctatct
      421 tggatatatc tattctcata gtgatgccct atctcatgcc tgtaacgccg gccggcggaa
      481 cgttataacc tgctgcgaat tcttcttgtc gtcatctcat ccggtgtttc gtatgccgcc
      541 ggcagctact tcgtgggctt cctgcctcga tgactgaatt tgtaatatca gactacaaat
      601 aaatatgtca gttatcaatg tttttatgac acaaaaaatg gatatgattg tttttgtgtg
      661 agatttggtg atttttgggt ataaaaaaag ccgctgtaag cggccttctc agtggcaaga
      721 tttagtcttt ttcgaatgga ggggggattt ttcgcttact caggaactca gccataaacc
      781 gatctaactc ttccagtcgc gttctcgcta gttcaataaa tctgtcctgc tccacttcag
      841 gaagttgatc aaatacttct aataactcta attgcttctg atttagtact gttttgctgg
      901 ctgtgacagc ctgtaatgaa gactcttctt catccgtcat aaagaaccaa tacagtggtt
      961 ttccaagtgc ttgcgggaat aactctaact tttcctttcg cgggaagtta cctgtattgc
     1021 accagttact gactgtctga gagtttacac ccactcgccg gcctaactca gattgagata
     1081 taccagcctc atcaagagct cttaacagcc gttcttcgaa gttcatattc gcatccaaat
     1141 caaaccagta accaagcata caaagtttct tagcacttgt gactgattaa gtttcttgac
     1201 attgacaaat tatttatcaa atatgtgaca aatttttagg aggaaacatg caagaaacgg
     1261 ttcaaaagaa aatcatttcc ctttgtggca gtcaatctga actagctcgc cgtttgggga
     1321 agaactctca aacagtatcg gtttggtttc gaactcaggt tgcaagtact gaggtactca
     1381 acgcatgcag ggttctggat tggcaagtga cgcctcatga gcttagaccc gatctttacc
     1441 caaacccgac tgacggactt ccaaaggact aaacatgcaa accatatctt tcaaaaatca
     1501 tacttctatg ttgggtatgc aacagaaaac ggaaaatcag tattcaccac ggcgtcggga
     1561 cagcgttaag tgccggacca tctttgcagc agttcgtgag tgggaggcaa ccttacccgg
     1621 gcgcgcgcag gagcacgtcg cacagctggt ggccgaacag tgggagaaac aagacggtcg
     1681 cggcatcagc gttaataaac aaaacctgta ccgctacctg aaaaacgaat ctggatcaga
     1741 gaagtacacc agttacgtca tgcagctttc aggagcgatc gctgatgcaa tgcctattga
     1801 gattgcgcgc aaacacggat tgaaacgtgg attgactgaa agcgagctgg tggctcaagc
     1861 aatcaaagag tgtagcgaag cgcaccaggc aaaattgctt ggcgctccgt tgcaaaagtt
     1921 agaacgtgaa attcgggagg cagcaattgc actttttaac atgcttcctg cagatgcggc
     1981 gggaccacta ctggcgagca taagcgccgt agcgccacag tttttttaat cgagttttga
     2041 caatgagtac cgtacagaaa ataggggggc ttcatgagca ttgacgcaat gcgatgggcc
     2101 aaaaaagtca agacagggaa gtcctctgca aaagctgttc tgacctggat ggccgacatg
     2161 tgcggagctg atctctgtgc ttttccatcc attcctgcgc tggcagaagc aactgagctg
     2221 gataagaaaa cggtccagtc gagcctgcag tatctgatct cgatcgggct tattgaagat
     2281 acaggtgaac ggcgtggaaa gactaaacaa atcccggttt accgacttct tggtgtggaa
     2341 gaaagcgttg ctgaaattga acacacccaa aaacgggaac attaccaaaa acgggatcgt
     2401 ttaaacattc ccgaaaacgg ggttgttaca gctgaaaagg cacccgaaaa cgggactgtt
     2461 tcctgtacgc aaaacaacca aacgatcccg ttttttccgt caaacgatcc caaaaacggg
     2521 atccggaatc taccagagga accaaaagat ataaccccca cacatagggt tctggtcgaa
     2581 ccagttgtgc ctgactatcc gaatcaaccg ggaatagttc ctggtgaaac acaagcattc
     2641 ggaaaatttg cgatgtattt cgggtggaaa ccttccgagg attttccccg actgtcaacg
     2701 atttggggaa tgccattaag accggggata aatcttgctg ccgagttaag cagcttcatc
     2761 gcgtactggc aggttgaagg cagggcgttt caccaggtcc agtgggagca aaaactagca
     2821 agacatctta accgtgcgga agtccgccag aaaaaaccag cgaacgggag taacgatcat
     2881 gtgggagtac gagcagagcc agcagcatcc agagctgttc aacagattcg agccgcccgt
     2941 gagcaacggt tgcgagttgc aggaacagac ggccgtagaa acggcgtggc gcctatggga
     3001 agtgatgggc gaaatctttt cgaaccgatg gatcctgaag aacggagagg aaccatcaga
     3061 gctctggatc gctcagattg ggtcgatgag tgaagcccag attacgctgg tttgccggca
     3121 gtgcatggag cgttgcgccg cgggcagcac atggccgccg gatctcgctg agttcgttgc
     3181 gctggtatct gccagcggtg ctaacccgtt cagtctgaca tccgatgcag tgatggcgga
     3241 gtacaaacgc tggagaaacg agtcttaccg atattcggga agcgacaaat acccatggaa
     3301 acaggatgtt ctgtatcaca tttgcattga gatgcgcaga actggagttg agaggaacct
     3361 gacggaggga gagctgaaaa aactggcaga aaacttactc acgaaatgga ccaaacacat
     3421 ggctaacggg ttttcgattc ccccgattcg tcggcagttg gcagcaccga ggcatccagc
     3481 agggccgacg ccagcgcaga ttctgatggg agagtacaaa cgccgcaagg ctgcaggttt
     3541 aaccaagtaa acgagttttg accatgacca aacaatcaaa aaccaaagta accaaagcgc
     3601 agatggtgct tgccatcgtt agccggacgc cagaatgcgt cctgcaggat gtctgtgatg
     3661 cgctggactt gcaagccagt acagcaggta acttgctgcg gcagctacat gccgcgggaa
     3721 aactccatcg tacccataac ggctgccagt acgtctatcg agtgggtgca ggcgttgagg
     3781 ttcccgatgt tgccctgccg caggctgcaa cacaattatc tgaagaagat gtgaaaaaag
     3841 tccaggacgc actgtcactg gcgaagatgc tggaagacaa aaagctgtgg cgccgggctg
     3901 cgactgttta cacctcgatg cttgggatgg ctacaacagc aaacgaactc tggttgcttg
     3961 ccagaatgcg taaccgctgc ctgcgcaatg tggcgaggtg gtgaatatgc cttaaataga
     4021 atcaacagca gctggtacgg gatgtcaaag ctaagtttaa ttattccggg gtgaggcaaa
     4081 gctgagatgt ccgctgagtg ccaacaacgg acattatgga aggtcagaat aaaagattaa
     4141 aatgggatgc tgtgtgcaaa aaacgcattt tttgcggttt tctcaaggtt ttgctctgca
     4201 ccttcctggg atactccacc aacagaatac gaaaatcaat attatcaacg atccggaagt
     4261 gtctagatta tccgtggcta ttcactatag ggcaggatcg tctaccgggg cggggtcagt
     4321 ccatctaggg atgggaaatc ccatcctagg gaaggtgcga acaagttcct gatatgagat
     4381 catcatattc atccggagcg catcccagag ggacatcatg agccatcaac tcaccttcgc
     4441 cgatagtgaa ttcagcacta agcgccgtca gacccgaaaa gagattttcc tctcccgcat
     4501 ggagcagatt ctgccatggc agaatatgac cgctgtcatc gagccgtttt atcccaaggc
     4561 gggcaatggc cgacggccct atccgctgga gaccatgctg cgtattcact gcatgcagca
     4621 ttggtacaac ctgagcgacg gtgccatgga agatgccctg tacgaaatcg cctccatgcg
     4681 cctgtttgcc cgattatccc tggatagcgc cctgccggat cgcaccacca tcatgaattt
     4741 ccgccacctg ctcgagcagc atcaactggc ccgtcaattg ttcaagacca tcaatcgctg
     4801 gctggccgaa gcaggcgtca tgatgaccca aggcactttg gtggatgcca ccatcattga
     4861 ggcacccagc tctaccaaga acaaagagca gcaacgcgat ccggagatgc atcagaccaa
     4921 gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg ccaagagtgg
     4981 cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc agctgggtaa
     5041 tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag gagcgccaca
     5101 gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgtc ccggcaaggt
     5161 aaaaaccttg aagcagcatc cgcgcaagaa caaaacggcc atcaacatcg aatacatgaa
     5221 agccagcatc cgtgccaggg tggagcaccc gtttcgcatc atcaagcggc agttcggctt
     5281 cgtgaaagcc agatacaagg ggctgctgaa aaacgataac caactggcga tgttattcac
     5341 cctggccaac ctgtttcggg tggaccaaat gatacgtcag tgggagagat ctcagtaaaa
     5401 accggaaata acgccagaaa tggtggaaaa aatagcctaa ataggctgat tcgatgtgtt
     5461 tgcgggaaaa aaatcggccc agatccgcga aattttaatc aacgagtcag cttgggaaga
     5521 aatgacctgc ttattcgcac tttccctaaa gcactttcta actcattcca ctcgtctcca
     5581 gcaggtatgt attcttctat cgcgtcatat agttctttgt cattttttgc tacatacgac
     5641 ttaaagtgtt tgtatcttgt cggtagtcca tgtcgaatgt caaatccgtt tcctattatg
     5701 taaagcctca tagcttttcc cttaatcgaa ttgtaaaaag attctaacca gacctgatac
     5761 ccgttgacta cccctcctcc ctttagtaat gccttcatca aatgtaaaaa catctgcgaa
     5821 cttccgcagt tcgctcaaag cagaccacat cctctgagcc tcacataatt gtccgccttg
     5881 taccaaaagc ggacggcatt aatctggctt ccatcacata ttcgaaagca ttttcatgtt
     5941 aatgggaagc gcttaagcct atgtcaggaa aaaggaatcc atattctaac tttaaatttg
     6001 ttgattttta tgtaaaatcg tggtaataac taatgataag ataatgtatc aaggaccaaa
     6061 ttgtggccga ttatataaac agcaacatat tatgtgagtc atatgttcat gttgagccag
     6121 aatggttgaa acaggtaagt gctgtaattc gtgaagaaaa gttatcacta attagaaacg
     6181 aaatagagga ttttgctgac aaaagaataa aattcttttt atctgatgat gttttaattg
     6241 aaattaaatt tactgaaggt tcaattaaag caaaggtaac tgcttatggt ccaattattt
     6301 tattgatcgg ttctgcaata ttaaattact caagctttag agaaagtgtg aagcagttgg
     6361 cctctgatat gtatcgtgcg tcatcaatgt tatcctcaga aatgcttttc cagacaaact
     6421 ctagaaacaa gagtgaaatc cttcgtattg aagccagaaa aggaatcgtt gggtccatag
     6481 agagaattaa taataaaatt gattccatta actctcaatt agatggtaaa gaactatcac
     6541 ccacacgtat aaatagcgag ttacttgatc taaatgatat gatcctaacg ctactatcga
     6601 acctaaaaaa cgcagaagat acaaaattag ttgcttcatc attaagtgat ggtgtatcta
     6661 aattatcaat acgtaaatct cgctttaagc tcaagaataa gctcgatgaa tcaatgtatg
     6721 caagcctctt taatgagaag aaaagtatta ttaaaaatct cgcccaatat aaatgatgag
     6781 aggcggggac actggtttca ttcgcgtccg tatgtccact ttgtgccagg agcggacctt
     6841 actaagttca cacaacattg attattgaag attaggtcat aaatatatac gtcattttgc
     6901 taatttagtt acgttggaac accgcgctgc ataaaaaata aggaataata tgactcgaag
     6961 tgaaataaac gtctgggatg tgagacgagg caccaacagt cctcgagttg aggagatccg
     7021 atctgaagct cagcgcgacc tttctcgatt gattcactcg tctcctttca gacgattgca
     7081 atcaaagaca caggttcttg gtctgggtga aagtgatttc tatcgcacac gactgactca
     7141 ttctatggaa gtcgcccaaa tcggttcagg tcttcttgct aagctaaaaa atatatataa
     7201 aggtaaagag gaagcgcaat ttttaccaga taacgatctg atgcaagcag tatgtctcgc
     7261 acatgatatt ggacatcctc catttggtca cggcggtgag gtagctctta actattgtat
     7321 gctggattat ggtggttttg aaggaaacgg ccagacgctt cgcattctct cgaaactaga
     7381 taaatatacc ttagaacacg gactcgatcc tacacgcaga cttctgctag gcatactgaa
     7441 atatccggtt aaatactcgg aactagtggt taaaaaagac agtatcccgg ccactatcgg
     7501 atcgccgaac tggctcttta agtcatctga ttttaaacct ccaaaatgct atctagattc
     7561 agaatgcgat attgtagaat ttgctctctc accatttact gaacatgatc ggaggctttt
     7621 tacttccgtt aaagatggtg aaaccaaatc cgatcataaa aaagcttctt ataaagctct
     7681 cgatacaacc atcatggatc ttgccgatga aatttcgtat tcgctgcacg atttggagga
     7741 tgctatttct ctcgggatgg tgacacgagc aatgtggatg gagcatttca gtggacatga
     7801 aaccttattt actgactgcc atggaaaaac attttccgga acatgtgaat ctgtcgcgga
     7861 tagccttttt ggtaaaagtt acaagagaaa agagtgcatc gggatgctgg ttcacctgat
     7921 gattacaagt gttgagctta atacaaagaa tgcagcattc aaatgtggca tgctcaaata
     7981 taatgcggtc cttccggaag cggtcgaagc actaaggttg aaaattttca aactggtcga
     8041 aaacaaagta attaagcatg aaaatgtaca gcagcttgaa ttcaagggac aaaagttgat
     8101 tgttgagctt ttcggtgtgc tggctaacga tccgtgtcgg tttctacctg cctcaacgag
     8161 ggaaatttac gagcagcaga ttagcttaga cgagaaggct ggtatgcgag tcatttgcga
     8221 ctatgtttca gggatgactg atggctatgc aacaaagctt tatgagaaga tccttatccc
     8281 acgcaaaggg tctatttttg accatctctg acgtggcaag aatgatgctc cgagttcaat
     8341 tggcctataa acattaacca aggcatctac cagggggata acaaatcccc cattctgaat
     8401 ttgttcgtgc agaatcggtg aacgttctca tctgagcgtt aagcgccagt aatcgttgat
     8461 ctacactgtc cattaataca ccttaatatc agcagcgtct tactccgtta tgagaacaat
     8521 cgcgaacttc tgctgttcgc tcgaagcaga ccttcatctc attcagattg tccgctctgt
     8581 gccaagagcg gaatctggag
//
