LOCUS       NZ_CP130679             4579 bp    DNA     circular CON 01-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KP9 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP130679
VERSION     NZ_CP130679.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36621066
            Assembly: GCF_030552975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Han,R.
  TITLE     Complete gene sequence of KP9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Han,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2023) Department of Pharmacology and Toxicology,
            College of Veterinary Medicine, Henan Agricultural University,
            Longzihu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2023
            Assembly Method                   :: SPAdes v. v3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 16:18:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,401
            CDSs (with protein)               :: 5,401
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 53 of 156
            Pseudo Genes (incomplete)         :: 99 of 156
            Pseudo Genes (internal stop)      :: 23 of 156
            Pseudo Genes (multiple problems)  :: 19 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130679.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP9"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China"
                     /lat_lon="34.44 N 113.42 E"
                     /altitude="108 m"
                     /collection_date="2021-05-30"
                     /collected_by="Pharmacology Toxicology Laboratory, School
                     of Veterinary Medicine"
     gene            join(4389..4579,1)
                     /locus_tag="Q4E97_RS28355"
                     /old_locus_tag="Q4E97_28355"
     CDS             join(4389..4579,1)
                     /locus_tag="Q4E97_RS28355"
                     /old_locus_tag="Q4E97_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1..399
                     /locus_tag="Q4E97_RS28360"
                     /old_locus_tag="Q4E97_28360"
     CDS             1..399
                     /locus_tag="Q4E97_RS28360"
                     /old_locus_tag="Q4E97_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_121576903.1"
                     /translation="MATVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFASVSVEQEEEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(406..1104)
                     /locus_tag="Q4E97_RS28365"
                     /old_locus_tag="Q4E97_28365"
     CDS             complement(406..1104)
                     /locus_tag="Q4E97_RS28365"
                     /old_locus_tag="Q4E97_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(1101..1988)
                     /locus_tag="Q4E97_RS28370"
                     /old_locus_tag="Q4E97_28370"
     CDS             complement(1101..1988)
                     /locus_tag="Q4E97_RS28370"
                     /old_locus_tag="Q4E97_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_048290304.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            complement(1981..2202)
                     /locus_tag="Q4E97_RS28375"
                     /old_locus_tag="Q4E97_28375"
     CDS             complement(1981..2202)
                     /locus_tag="Q4E97_RS28375"
                     /old_locus_tag="Q4E97_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            complement(3261..3377)
                     /locus_tag="Q4E97_RS28380"
                     /old_locus_tag="Q4E97_28380"
     CDS             complement(3261..3377)
                     /locus_tag="Q4E97_RS28380"
                     /old_locus_tag="Q4E97_28380"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_016947047.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRTFRRRR"
                     /name="small membrane protein"
     Typing          complement(3866..4341)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="2.7e-180"
ORIGIN
        1 atggcgacag tgtcgatcag tgaggctgca cgcctgacgg gtaagagcag gacaaccctg
       61 cacaggttaa ttaaagccgg tgagctgtcc acctgtcggg gtgaacggaa tgcgaaaatg
      121 ctcgatactt cagagctttt gcgggtgttt ggtgcctttg cgtccgtatc cgttgaacag
      181 gaggaagaac aggtcagtgg acagcgtgac acagtcactt ctgaccataa tgaacaggtc
      241 attcatactc tgaaacagga aattgaacac ctgaacaccc ttcttgcctc aaaagattca
      301 catatcgaca gtctgaaaca ggcgatgttg ttgctggaga accgggaaga tcagcggcag
      361 aacacgaccc ggccctggtg gcgtttctgg cagtcctgaa atgcctcagt gtttttgccc
      421 tgctgttccg ccagtggcct ggagtttttc cagtttccgt ctgcgccgtt ccgccgtcgc
      481 gcaggcattt ctccgtcttt cctctgcttt ctctgcttcc agacgggcca gctcggtgtt
      541 atgccgctcc ccggccacat atttccgggc cttctccagg gttgtttcca gcgccagctc
      601 ctttttctgc aggatggatt cgcgctgatt cagtgcctcc ctctgttctt ccagctcctt
      661 tctggcttct ttccgggcat tctgcaccgc ctgaagcaag aggctttctt tttgcgcaaa
      721 cgcttcccgc agtacatcct cagcaccaat gtagtggctt cgcagggccg ctgtctccag
      781 gttgtgattc tgaataaggg tattttgtct ggctctgtga gcctcttctg cctccctcag
      841 tgcctgctga tatccgctta tctcgcgctg tatccggcgg gactctgagc ctctgaaaaa
      901 caaaatgaac agccacagcg agaacgctgt ggctgcgatc cccgttgcca ggatagcggg
      961 accgagttcg gtgaccgcct tccagccagt aaacgcctca ggattgctga cgtccggata
     1021 gtaaacgcca tagtgattca gctcatacag ggcgtacgcg atggcgaaaa caccggtaca
     1081 caataaaata aatgttcttg tcatcgcagg tatgtctccg tgatttccgg acgaaaatgg
     1141 cccatttcct ggctgacggt gcgcagggcc atatcacgaa ccagaccgag tttttgcagc
     1201 tcctgcatcc gttgctgggc gtagctgtga cgcacgccgt gtgccccccg tgaccagccc
     1261 agggtacgca tggaggcggc actgaaggcg tttgaccagc gctggcctcc gccaatggcg
     1321 tactgactct ggtaaaacac gccgcgatcg gtcacccgga ccgggctggc cagcctgaca
     1381 tgctcgagac gttcagccag gcgtgacggc agcagaactt ccctgaccag gccgcctttg
     1441 ccctgaacgg tgtaaatcac accctcccga ccggcaaatt tactgtccgc tgccggacgg
     1501 ggatccgctg ctcgctcctg gcggggcgcg agggtcagca gttcatgggc gcgtaatccg
     1561 gcggccaggg caatcgcggt ggccagagcg ttggcgggct gttgtgctgc ggcaatcagg
     1621 gctacctggt ccgggctgta ggcccggctg ctcaggatct gtgcctgctc cgatttcacc
     1681 accggcaggt gttcgccggc agggagggtc tgcgtcacgt ggatcatcat ggcctggatg
     1741 gcctggcgtt ccatatcgag cgttttctgg ccaacctgct ccccgcgctg ctccaggtag
     1801 gtgatggcct gcgccggtgt cagtgtgcgt aaatcgccgt gattaatccg gttggctttc
     1861 atccactctg cgacgcgggt caggccctgc tcgtaattcc ggactgtgcc gacagagcgc
     1921 agaatatttc cctggagtgg ttttatcacg ccagccgcct ggcgggcagc gctggcgaat
     1981 ttagccattt ttttccagcc agcgggtaac cgtggacaac accaccttga ttcgccgggc
     2041 ccggagccag cgttgcagct ccgctgcact ggccccggcc tggtacaggc tcagcagttc
     2101 gccggtatag cggtcaaggc gggagggacg gtagcggacc tggcggatac ggcgggtctg
     2161 cgcctgcagg gcggccagtt cggcagcggt atcaaattgc atctgtgctc cggattgggt
     2221 gtaagaacat cagtggccgt tacgttaacg gtcactgcgg catgcgccga tcgccctcgt
     2281 tcgaacctct cgtggttcag cccggactgc tcgcggctga taacgtccct taagccaggg
     2341 aatggcctga cctgattgcc ggtctcgcag attttagctg ttttctcagg attctcggat
     2401 tgggctgccc ggtacagtat ccccggaggg acccggctgt tttacctgct gccggtaggc
     2461 aggtgcgcgt cagagtgcgc ctcctcatgg tttgactaaa tactagccag gtttttcgtg
     2521 cgtgtgaatg ttgtttttta aatgttgcgc ctgactccgc tcaatagcga cacccgctat
     2581 gtacaggcgc atttatgcgt cgtacatagc gggtgtcgca ggtgctgatt tggcggaata
     2641 aaaaacaccg ggactgaccg ccccggaccc cgccaacggt gaccggacag acaaaaaatt
     2701 tgcctgcccg gtcaccgttt cacatcatgg cgaggaggcg ggatctgaaa cggtgtgatg
     2761 aaagtgcaga tgaggtcaga cgatgcaggt cggtgagtcc gtggtgaacg cgatagtgga
     2821 ataccacgca cagggcagtg aggaggcgca tgcagaggaa aacaggatgt tcctgcgtta
     2881 tctgcgcgag ttaacgggaa cacagcgtgt ggcggttgag gtggccagga aatatttttc
     2941 gccggtgagg ggctgatatg agctaccttt ttcttgtcgc cggcaccgca ccaggtgcga
     3001 cggtcctttt acagagttgt cctcatttga cgggcctgga tggcccgttc ttttatgctt
     3061 ttaacgtccg gggccctttt ctcgaccgaa atcacctact atgcagtggt gtgaatagcc
     3121 atgcatatgc gtaaaataaa tcaggcgcgt ttctggcggt tttcggtgta cttgccacca
     3181 ccttttgccc gttaaccacc agaaacgccc tgaggccgtt ttcgcggtgc gcgtaagaag
     3241 ggattatggt aaatagggcg ctatcgccta cgcctaaatg tacgactgat tgtttttttt
     3301 tctctgaagt atgagattcc tgagtagaca gctacaacaa gtagagcaat ggcgatagca
     3361 agtgaaatcc agtccataaa tgcctgatta atttaataaa aacagcctgt tatgatttca
     3421 taaagataat atcatctttt tttagcatga tactgtcgtt acttattgtg gtgagatgaa
     3481 acatagccac tgtttttgta tgtgtttgtt aaggctgcta agattattcc taaccactga
     3541 ttttgtacat atcatcaaaa aaataaaaaa actaatgccc tgtggtgggt atgtaacaaa
     3601 tcacggggag cgggtcagaa ctacgccaaa actttgaatg atcggggggg cagactaaat
     3661 gaaatcttga gattcattca gtctcgtcgt aatctcttgc tctgtaaacg aaaaaaccgc
     3721 cttgcagggc ggttttctcg aaggttctca gagcagcaac tctttgaacc gaggtaactg
     3781 gctagttaga ggagcgcagt caccaaaaca gttcgtttag tttagcctta actggcgcat
     3841 gacttcaaga ctatctcctc taaatcagtt accagtggct gctgccagtg gcgcttttgc
     3901 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc agtcggactg
     3961 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcccgg aactgagtgt
     4021 caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     4081 caggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat ctttaagtcc
     4141 tgtcgggttt cgccaccgct gatttgagcg tccgtttctg tgatgcttgt caggggggcg
     4201 gagcctatgg aaaaacggca acgccgtggc cttttccaca ggctcttctc ttcctggtta
     4261 tcgcgatgac tcttctgtga gtcccgtccg ctcgccgcag ccgaacggca ggagcgtagc
     4321 gactgagtga gcgaggaagc ggaaaagaga ccggttttac actgagcact gacgccctga
     4381 ggccccttat gacgaaacaa cagaaaactg ccctgaacat ggctaaattc atccagaatc
     4441 agtccctgct cctgctggag aagcttaacg agcttgatct cgatgcggag gctgaccttt
     4501 gtgagaaact ccacgatgat gcggagcatc ttttccgtac actgtccctc agactggatg
     4561 cactacagga gggtgactg
//
