LOCUS       NZ_CP130678             4991 bp    DNA     circular CON 01-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KP9 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP130678
VERSION     NZ_CP130678.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36621066
            Assembly: GCF_030552975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Han,R.
  TITLE     Complete gene sequence of KP9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Han,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2023) Department of Pharmacology and Toxicology,
            College of Veterinary Medicine, Henan Agricultural University,
            Longzihu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-JUL-2023
            Assembly Method                   :: SPAdes v. v3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 182.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 16:18:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,678
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,401
            CDSs (with protein)               :: 5,401
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 53 of 156
            Pseudo Genes (incomplete)         :: 99 of 156
            Pseudo Genes (internal stop)      :: 23 of 156
            Pseudo Genes (multiple problems)  :: 19 of 156
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130678.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP9"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China"
                     /lat_lon="34.44 N 113.42 E"
                     /altitude="108 m"
                     /collection_date="2021-05-30"
                     /collected_by="Pharmacology Toxicology Laboratory, School
                     of Veterinary Medicine"
     gene            1..1059
                     /locus_tag="Q4E97_RS28325"
                     /old_locus_tag="Q4E97_28325"
     CDS             1..1059
                     /locus_tag="Q4E97_RS28325"
                     /old_locus_tag="Q4E97_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(1089..1346)
                     /locus_tag="Q4E97_RS28330"
                     /old_locus_tag="Q4E97_28330"
     CDS             complement(1089..1346)
                     /locus_tag="Q4E97_RS28330"
                     /old_locus_tag="Q4E97_28330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            1764..2195
                     /locus_tag="Q4E97_RS28335"
                     /old_locus_tag="Q4E97_28335"
     CDS             1764..2195
                     /locus_tag="Q4E97_RS28335"
                     /old_locus_tag="Q4E97_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(2289..2693)
                     /locus_tag="Q4E97_RS28340"
                     /old_locus_tag="Q4E97_28340"
     CDS             complement(2289..2693)
                     /locus_tag="Q4E97_RS28340"
                     /old_locus_tag="Q4E97_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(3488..3889)
                     /locus_tag="Q4E97_RS28345"
                     /old_locus_tag="Q4E97_28345"
     CDS             complement(3488..3889)
                     /locus_tag="Q4E97_RS28345"
                     /old_locus_tag="Q4E97_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3889..4080)
                     /locus_tag="Q4E97_RS28350"
                     /old_locus_tag="Q4E97_28350"
     CDS             complement(3889..4080)
                     /locus_tag="Q4E97_RS28350"
                     /old_locus_tag="Q4E97_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4202..4604
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="99.0"
                     /evalue="1.66e-166"
ORIGIN
        1 atggcgatct atgccgtatg gaataacaag ggtggtgtag ggaaaagtta ccttactttt
       61 cagttagcct ctgaatatgc cagacagaac cctactaaaa aggtcctggt ggttgacctg
      121 tgcccacaag cgaactcgtc ctctatgtta cttggtggaa tgataaatgg cgaaaacaca
      181 ctaaaccaga ttcattcagc aatacagaga aagacaatat ctggatatat tgaggatagg
      241 atcagaagtc catatattcc taccaacact ggttctagct atgtaataaa tgtatctaca
      301 cacaatagta aagttccaaa taatctatat cttgtcactg gcgatgaaca attagaactc
      361 caagcatcaa gagttgttgg ggcatcattt ccaggtcccc atgatgcatg gcgtactgtt
      421 cacctctgga ttaatgattt aatcagtgac atttgcaact catggaataa tgaagatagc
      481 tgcgtattca ttgactgcaa ccctagcttc tctatttata cagagttagc tttaaccgca
      541 gcagagcgcc ttttaattcc tttctctgcc gatggttcat caaagagagc agttaaggct
      601 gtactttctt tagtttatgg tgagcgtcgc catcagaatg aaccacaatc agatttctat
      661 cttaatagca atcgttattc tatgtcgcta ccacaaatat atatgtatgt tggaaataga
      721 ttaacgcaaa tgaacaattc atctgccagt gcatttaaga cggtagtaac cgaaattggt
      781 gatgaaattt ggaaagtatg gcaaacaaaa ccaagtctgt tttgtattca cccaaatggg
      841 agctcaacgc caaataataa aagatctttc aggaatatgt ttcagtatga agtaaatgat
      901 gcaaacacag catctgtcgt atctggagcc ttaggtattc caatcgcgac attaactgca
      961 ggaaataaaa ctgtttcagg aaaaataata aaggttaatc agacgcagct agataaacaa
     1021 gtccccaaca ttgtagctct cgctggtatg atcgaataaa atagcaaggc ccttaaaagg
     1081 gccttttttt atttgttttt ctttactgct tgcagcgccc tgccagcact agaggccaac
     1141 tctttgatta cattatcttt actttcaaca acctcagtaa gcaactcgtt ttcgttttcc
     1201 agtttattca aagcgttctg agtatcttca atagcatcat catacccttc ggcatagcca
     1261 tccatccaac caagagtata cgtttggtcc aaaagatcag cttgcttatc ctgcagatct
     1321 ttattctgat tattgttaga gcccatgttt tttcctcatt tgttggcgca tctgagtttt
     1381 caaaccagga ggaattccat ttctaagttt ctcacttagt ctgactggtg gtgtgtttcc
     1441 tgagactgga ttacagtcag catccacccc gacatctttc tttttatagt cgccaatatt
     1501 cctaacgtta tctgtagtca tagaaaacca cgcccattgc tgtgaataac cgaactataa
     1561 accagaaaaa aaacgtacgg cttatcataa cacttgacac aaaaaaacac cgctactctc
     1621 caagcaaaaa acataaaatt ggaaaaacaa aatcacaagc acatgataac aatagatatt
     1681 aatatttact tacatttagt gagttttata attagaaaaa caatgtaatt taataatcac
     1741 aaacattacc taaggacgaa ctgatgaaaa tacccgcgca tttgaagcat aaaccaatta
     1801 ttcaagtcga aaattacgac cgcattgacg gcccacatgc cgatgacact gatgctatgg
     1861 ggctatctgt aggtattgct caatggaaca ctcctggatg gactgagctt tcggcaaagg
     1921 tttggcgaaa cacaggagaa aaatggtcaa gacaatcaga agagctgcct cttcaccggg
     1981 taatcgacct tgcaactctg atctgtatca ccatggacta cagtaaaaat gggcgacttt
     2041 catccagtga tgattttccc gtgactcgct caactgataa ccctgagcta gcacatcaca
     2101 ctgagtttat gaaaaaagag ttaaaacaaa ataaaagcaa tcttgatgta agcctcaaac
     2161 gcctctcgac tgaattgaag agacttggct attaaaacta gctttagaaa tgtctaccta
     2221 cttcacagac gaatttacca taacatccgt ttttcgaacc ataatggcag gctatagcct
     2281 gccgtaattc accggaaaaa atcatccaca gagtcgctcc actcatgccc ggaaagagac
     2341 cacaccttgt ttataggggg aggataacca ctcctgcagt acccgttttt tcccacgaaa
     2401 tcgaatgccc cgtgcgggta tccattgatc aggagtacgc acttctgact gtcttctgac
     2461 cagccaattt taacatcaga aggaatgtga ccgtctgaaa cgtcttcggc attgtagatg
     2521 tgtactgcgt ccataatcgg gtttccctcg acggactcat cgagcgcata gaaatacccg
     2581 gtccggccat cgtcttcgaa cacggcggcc agcatgccct caggagcgag gctctttata
     2641 acctgttcct ccccgacaac gagctgggcc tgtatcgtca ctaaaatggt catcaggtct
     2701 gttctccttt ccttggttgc ttcggcagcc agctttcaga caagcggata atttaccata
     2761 acatccgtta tcagcatcgt cagaaacgcg ctgagggcgt ttctgacggg aaaccggtaa
     2821 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttatggcat
     2881 gcatgagtat gcacgcaagt gcatagcgcg aatattcacg ccattcagac agggtaagtc
     2941 ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     3001 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag cacgttcagg aatcgggttc
     3061 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
     3121 gcgactctgc actagagtcg tacctagagt cgccgcgact aaaaaacaac caaacgggga
     3181 gtcgggtcta gagtcgggtc gttattaact cattgttttt atagccctga gtcgttaccg
     3241 actctatgac gactcgccgg ggactctatg acgagtcgtc atagagtcgg atctatataa
     3301 ataaaattta aaaaacgaaa tctcaggaaa gtaaactact gatattaaat acaaatctgc
     3361 aaaattttaa ttttgcgtgg ggtgtgggta tttttagtgg tgaggagcgt tcagagatct
     3421 gactattttt gggaaggttc aggaactccc cgggatggcc ggagaggaaa actgaagcat
     3481 gacagggtca ggattttttc cagaatttcc accatggcgc cggggattca gttaattttg
     3541 cctggtgatt ttcaagaagg agcatggcct gtttcaggct gtcaatgtgc gaatctttgg
     3601 acaaaagtaa agtgttcagg tgttcaatct cctgtttcag atgacgaatc acctgttcac
     3661 actggtcaac agaggctgta tcgcgctgtt cagagacctg tccagaatgg tgttcaccct
     3721 tcagttgttc aaagggacca aagacacgta aaagctctga agtatctacc atttttgcat
     3781 tgcgctcacc gctacaggtg gacaggtcac ctgctttaat taacctgtgc agtgttgttc
     3841 tactcttacc cgtcagtcgg gctgcctcac taatagacac tctggccatc agttaccatc
     3901 ctgtaatgca tccagtctcg aggacagtgt acggaaaaga tgctccgcat catcgtggag
     3961 tttctcacaa aggtcggcct cgacatcaag atcaagctcg ttaagcttct ccagcaggag
     4021 cagagattga tgctggatga atttagccat gttcagcgct gttttctgtt gtttcgtcat
     4081 aagaggcctc agagcgtcag tgctcagtgt agggatgttc tcttttccgc ttcctcgctc
     4141 actcagtcgc tacgctcctg ccgttcggct gcggcgagcg gcaggagcat gcgtatgaaa
     4201 gctcgagaaa accgagcgat cacgtaatgt agaaaaggga aggccgcaca gcggccgttt
     4261 ttccataggc tccgcccccc tgacaagcat cacagaaacg gacgctcaaa tcagtggtgg
     4321 cgaaacccga caggactata aagatatcag gcgtttcccc ctgatggctc cctcgtgcgc
     4381 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     4441 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     4501 cgaacccccc gttcagtccg actgctgcgc ctgttccggt aactttcgac ttgagtccaa
     4561 cccggaaggc acgacaaaac gccactggca gcagccactg gtaaggggtg tacaagaatt
     4621 actggtgcag agttcttgaa gttacagcct aactacagct aaactgaaag gacagatttg
     4681 gtggcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac ttactgatcc
     4741 ttcgaaaaac cacctcccca ggtggttttt tcgtttacag agcaaaagat tacatcccgc
     4801 aggttaggat ctcaagaaga tccttttaat cggaattcga caactccaga taagtcatgg
     4861 tgagccagcc aaccccttcg actgaatatc gttccaaaaa aaggcagtca gctgaaagaa
     4921 actaacagta gaacaataat ctctagtacc ctgaatctaa gctaacaaca aaactagatt
     4981 aggagttata g
//
