LOCUS       NZ_CP130659            15598 bp    DNA     circular CON 01-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain SM117 plasmid
            pSM117_1, complete sequence.
ACCESSION   NZ_CP130659
VERSION     NZ_CP130659.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36664845
            Assembly: GCF_030552935.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 15598)
  AUTHORS   Bao,D.
  TITLE     Genomic characteristics of a carbapenem resistant Klebsiella
            pneumoniae isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15598)
  AUTHORS   Bao,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2023) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 16:17:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,225
            CDSs (total)                      :: 6,101
            Genes (coding)                    :: 5,800
            CDSs (with protein)               :: 5,800
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 301
            CDSs (without protein)            :: 301
            Pseudo Genes (ambiguous residues) :: 0 of 301
            Pseudo Genes (frameshifted)       :: 162 of 301
            Pseudo Genes (incomplete)         :: 151 of 301
            Pseudo Genes (internal stop)      :: 28 of 301
            Pseudo Genes (multiple problems)  :: 40 of 301
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130659.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15598
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SM117"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pSM117_1"
                     /geo_loc_name="China: Sanmen"
                     /collection_date="2023-01-19"
                     /collected_by="Danni Bao"
     gene            complement(join(15040..15598,1..5))
                     /locus_tag="Q4S50_RS00005"
                     /old_locus_tag="Q4S50_00005"
     CDS             complement(join(15040..15598,1..5))
                     /locus_tag="Q4S50_RS00005"
                     /old_locus_tag="Q4S50_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_304394109.1"
                     /translation="MTKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAI
                     IEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSF
                     TLAELVTKGHLRPLKEASEAENVA"
                     /name="Tn3 family transposase"
     gene            <2..106
                     /locus_tag="Q4S50_RS00010"
                     /old_locus_tag="Q4S50_00010"
                     /pseudo=""
     CDS             <2..106
                     /locus_tag="Q4S50_RS00010"
                     /old_locus_tag="Q4S50_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286714.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /name="transcriptional regulator"
     gene            934..1755
                     /locus_tag="Q4S50_RS00015"
                     /old_locus_tag="Q4S50_00015"
     CDS             934..1755
                     /locus_tag="Q4S50_RS00015"
                     /old_locus_tag="Q4S50_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="WP_004152492.1"
                     /translation="MRLASRFGLTHSIRQERPLTNDELVKVVPSVFSEEKHNSRSDRYT
                     YIPTITLLDKLREEGFQPFFACQSRVRDEDKRGHTKHMVRLRREGANKGTEVPEIILLN
                     SHDGSSSYQMIPGMFRFVCTNGLVCGTSFGEIRVPHKGDIVGRVIEGAYEVLGIFDKIT
                     EGVDVMKSIALTKEEQRLFGQAALTYRYEDENKSPVSIEQIIHPRRYEDKKDDIWTTYQ
                     RVQENLIKGGLPGRTEKGKRTTTRPVKAIDGDVKLNKALWLIAEKFRTLKG"
                     /name="DUF932 domain-containing protein"
     gene            1788..2117
                     /locus_tag="Q4S50_RS00020"
                     /old_locus_tag="Q4S50_00020"
     CDS             1788..2117
                     /locus_tag="Q4S50_RS00020"
                     /old_locus_tag="Q4S50_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="WP_013214015.1"
                     /translation="MVGILMFEFKAVCISISHMEEGDIRVLQVLSSTTNYLTCDSYLEW
                     IHDTPYGCVIRMYDFKRHLLALKRGGVSKFLRKTLILMHREGGYLLFHFDSDADYYPGI
                     DAVDF"
                     /name="DUF5983 family protein"
     gene            complement(2150..2635)
                     /locus_tag="Q4S50_RS00025"
                     /old_locus_tag="Q4S50_00025"
     CDS             complement(2150..2635)
                     /locus_tag="Q4S50_RS00025"
                     /old_locus_tag="Q4S50_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="WP_014343495.1"
                     /translation="MKGVAFGIILSLIPLSSMAADCFDMAGRDYKIDPDLLRAISWQES
                     GFKIDAIGRNPVTGYGSGLMQIDSQHFNELSRYGIKPDHLLSDACLNIYTGAYYLAQAF
                     KKWGVSWDAVGAYNAGFKKTPRQAARRYEYAKKIHYYYTAIKASKRTSSKDQKIAMN"
                     /name="transglycosylase SLT domain-containing protein"
     gene            3026..3442
                     /gene="traM"
                     /locus_tag="Q4S50_RS00030"
                     /old_locus_tag="Q4S50_00030"
     CDS             3026..3442
                     /gene="traM"
                     /locus_tag="Q4S50_RS00030"
                     /old_locus_tag="Q4S50_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229722.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="WP_014343494.1"
                     /translation="MFDAVFNKGTDMARVQAYASDEVAEKINAIVEKRRAEGAKEKDVS
                     FSSVASMLLELGLRVYEAQMERKDSGFNQTEFNKILLENVMKTQFTVSKLLAIGSLSPH
                     VTGDERFEFRSMVSNIREDAKDVISHFFPEQEEE"
                     /name="traM"
     gene            3642..4379
                     /locus_tag="Q4S50_RS00035"
                     /old_locus_tag="Q4S50_00035"
     CDS             3642..4379
                     /locus_tag="Q4S50_RS00035"
                     /old_locus_tag="Q4S50_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="WP_154244330.1"
                     /translation="MCAKDRSHIAVRSQTDSIHSMREMLNISSCPAFIRDSRGEVIHTS
                     PTFDKIFLTSGGTGAFDTPRAPGSWFLDLSLEIKLELMQSELKSFSEGSAVLVKNIWLA
                     GSLWTVFIETFSINEDTYSKWVFINEDEPIPHPSMEYNDFSIKMQRYIERIQRSAKSDW
                     AIFNLYAVGFSHASISKITGVGVQTSKNTVSKIKKELSFDDRDYIIMSSIYTLSYGKFI
                     SNVVSILKDGVNFLLNQKCHRYF"
                     /name="conjugal transfer protein TrbJ"
     gene            4494..4700
                     /locus_tag="Q4S50_RS00040"
                     /old_locus_tag="Q4S50_00040"
     CDS             4494..4700
                     /locus_tag="Q4S50_RS00040"
                     /old_locus_tag="Q4S50_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="WP_171773970.1"
                     /translation="MRSNARPAPGKNVLLKLDEETNRRLIKAKDRSRRSKTAEAYLRLK
                     DHLERFPDFYNSELAVPGGEKEE"
                     /name="TraY domain-containing protein"
     gene            4762..5130
                     /gene="traA"
                     /locus_tag="Q4S50_RS00045"
                     /old_locus_tag="Q4S50_00045"
     CDS             4762..5130
                     /gene="traA"
                     /locus_tag="Q4S50_RS00045"
                     /old_locus_tag="Q4S50_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="WP_004194426.1"
                     /translation="MNAVLSVQGSAVSEKTKPSFMSRVFNKKKALKVAKVALPVAAVAA
                     FFPDAAMATTTATDLMKSGDATVKGTFGKQSSVVKWVVLAEVVAGGIMYMMTKNVKFLF
                     GFAIISTFITIGMSVAGY"
                     /name="traA"
     gene            5144..5449
                     /gene="traL"
                     /locus_tag="Q4S50_RS00050"
                     /old_locus_tag="Q4S50_00050"
     CDS             5144..5449
                     /gene="traL"
                     /locus_tag="Q4S50_RS00050"
                     /old_locus_tag="Q4S50_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein TraL"
                     /protein_id="WP_004144424.1"
                     /translation="MTGDELKRYRFPETLTNQSRWFGLYLDELIPAVICLGWGFWTSKF
                     IFGIASAVLVFWGIKKLKKGRGSSWLRDLIYWYLPTSLLKGFFHDVPDSCFRQWIK"
                     /name="traL"
     gene            5469..>5834
                     /gene="traE"
                     /locus_tag="Q4S50_RS00055"
                     /old_locus_tag="Q4S50_00055"
                     /pseudo=""
     CDS             5469..>5834
                     /gene="traE"
                     /locus_tag="Q4S50_RS00055"
                     /old_locus_tag="Q4S50_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein TraE"
                     /name="traE"
     gene            complement(5889..6593)
                     /locus_tag="Q4S50_RS00060"
                     /old_locus_tag="Q4S50_00060"
     CDS             complement(5889..6593)
                     /locus_tag="Q4S50_RS00060"
                     /old_locus_tag="Q4S50_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            6618..6818
                     /locus_tag="Q4S50_RS00065"
                     /old_locus_tag="Q4S50_00065"
     CDS             6618..6818
                     /locus_tag="Q4S50_RS00065"
                     /old_locus_tag="Q4S50_00065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072354025.1"
                     /translation="MISLSPPTICNSARETALPESVLREPQREREAVREVARENLLQER
                     LQQMERDMVRDLQKEKTLGGD"
                     /name="hypothetical protein"
     gene            6838..7584
                     /gene="traX"
                     /locus_tag="Q4S50_RS00070"
                     /old_locus_tag="Q4S50_00070"
     CDS             6838..7584
                     /gene="traX"
                     /locus_tag="Q4S50_RS00070"
                     /old_locus_tag="Q4S50_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="WP_000205770.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLMV
                     LDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAY
                     YLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLM
                     LAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKS
                     LPRFWPGDFFPVFYACHLAVLGVLAL"
                     /name="traX"
     gene            7639..8199
                     /locus_tag="Q4S50_RS00075"
                     /old_locus_tag="Q4S50_00075"
     CDS             7639..8199
                     /locus_tag="Q4S50_RS00075"
                     /old_locus_tag="Q4S50_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="WP_000139328.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLAE
                     KAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRDVL
                     LEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEAYAA
                     ARLDKIRRQNRIKAELQAVLDEK"
                     /name="fertility inhibition protein FinO"
     gene            8330..8530
                     /locus_tag="Q4S50_RS00080"
                     /old_locus_tag="Q4S50_00080"
     CDS             8330..8530
                     /locus_tag="Q4S50_RS00080"
                     /old_locus_tag="Q4S50_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059022.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAARV
                     APRTDWQQFARQRAERCQRRR"
                     /name="hypothetical protein"
     gene            8916..9515
                     /locus_tag="Q4S50_RS00085"
                     /old_locus_tag="Q4S50_00085"
     CDS             8916..9515
                     /locus_tag="Q4S50_RS00085"
                     /old_locus_tag="Q4S50_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="WP_032083981.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNNV
                     ALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYDGI
                     VRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTFNNY
                     LKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
                     /name="PIN domain-containing protein"
     gene            9577..9909
                     /locus_tag="Q4S50_RS00090"
                     /old_locus_tag="Q4S50_00090"
     CDS             9577..9909
                     /locus_tag="Q4S50_RS00090"
                     /old_locus_tag="Q4S50_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152916585.1"
                     /translation="MAFISMTRQKAPYRYASKTRLSIRRTDSGSAGCYVTVAVRGKQEF
                     IIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTLPARFCREILPDDVRSITIILEKSDDE
                     WWYGSY"
                     /name="hypothetical protein"
     gene            10214..10363
                     /locus_tag="Q4S50_RS00095"
                     /old_locus_tag="Q4S50_00095"
     CDS             10214..10363
                     /locus_tag="Q4S50_RS00095"
                     /old_locus_tag="Q4S50_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001312851.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAY
                     EARK"
                     /name="Hok/Gef family protein"
     gene            10647..10895
                     /gene="repA"
                     /locus_tag="Q4S50_RS00100"
                     /old_locus_tag="Q4S50_00100"
     CDS             10647..10895
                     /gene="repA"
                     /locus_tag="Q4S50_RS00100"
                     /old_locus_tag="Q4S50_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083837.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKELR
                     VFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
                     /name="repA"
     gene            11140..11214
                     /gene="tap"
                     /locus_tag="Q4S50_RS00105"
                     /old_locus_tag="Q4S50_00105"
     CDS             11140..11214
                     /gene="tap"
                     /locus_tag="Q4S50_RS00105"
                     /old_locus_tag="Q4S50_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="WP_001375168.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGWCD"
                     /name="tap"
     gene            11207..12064
                     /gene="repA"
                     /locus_tag="Q4S50_RS00110"
                     /old_locus_tag="Q4S50_00110"
     CDS             11207..12064
                     /gene="repA"
                     /locus_tag="Q4S50_RS00110"
                     /old_locus_tag="Q4S50_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_032495102.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESDAGVLSITRATRALTSLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDVSEV
                     AVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDADRERQDIITLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            13003..13656
                     /locus_tag="Q4S50_RS00115"
                     /old_locus_tag="Q4S50_00115"
     CDS             13003..13656
                     /locus_tag="Q4S50_RS00115"
                     /old_locus_tag="Q4S50_00115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000616807.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLFF
                     SHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALALPA
                     EPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITS
                     LLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            13749..14006
                     /gene="pemI"
                     /locus_tag="Q4S50_RS00120"
                     /old_locus_tag="Q4S50_00120"
     CDS             13749..14006
                     /gene="pemI"
                     /locus_tag="Q4S50_RS00120"
                     /old_locus_tag="Q4S50_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_000557619.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
                     /name="pemI"
     gene            13939..14340
                     /gene="pemK"
                     /locus_tag="Q4S50_RS00125"
                     /old_locus_tag="Q4S50_00125"
     CDS             13939..14340
                     /gene="pemK"
                     /locus_tag="Q4S50_RS00125"
                     /old_locus_tag="Q4S50_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_001398199.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGT
                     RPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTID
                     MKARGGKRLERVPETIMNEVLGRLSTILT"
                     /name="pemK"
     gene            14589..>15004
                     /locus_tag="Q4S50_RS00130"
                     /old_locus_tag="Q4S50_00130"
                     /pseudo=""
     CDS             14589..>15004
                     /locus_tag="Q4S50_RS00130"
                     /old_locus_tag="Q4S50_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /name="IS1-like element IS1B family transposase"
     Typing          11310..12064
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5145..5446
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.2e-61"
ORIGIN
        1 gtcactcgtc agccggatcc tcagtggtga acgcagtctg accctcgatc acatgcgggc
       61 gctggcaaat cgatttcaaa tcccggtctc gatgtttgtt gactgattcg ggaacagagc
      121 aggcgaaggg ggcatctgtc cccgtctggc cgcgcctcga aaggccgcgc gcctgcggcg
      181 ccgggaaaac cgggccgcgc tgacgcgccg cccgcatttc ctttccgcgt cttccctgtt
      241 tgctgcctgc tgtctgtctg cttcctcttc ttctccctgc tgtgatgcct ctttttaacc
      301 cttaaaggct ttaaaagcac acccatactg gacaccttcg gctcctggcc agctacgctg
      361 tccagtcgtc gagccacact ccgctaaccg cgaaaagacg cggtaagctc cgccgaaccc
      421 gcaagcggtt tcgggcctga cggctagtgt ctgatgtctg aagtcaaggt caacggcagc
      481 gcttgctgcg accgtcccgg tcgcgccgct ttcaccgtgc cggcgaagga ctgccggcac
      541 tctgcagggc aacgccgttt cgcgcccgcg ctcttcgtcg ttgcggcggg tgcctgctcc
      601 tttcccctgg tacccgtatc acccgtttga gtccattcgc cggggctgag acaagcacaa
      661 cccgtcttgc tgtccagggg acggcagagc ctttcgcaat aaatttttcc cggccattcc
      721 gctgcgcttc attagaccga aaaaatttcc cgctcacccc cggacatctg caccgggccg
      781 tgctcgttta cgccctcgcg gcgaacggct ctcaaacggg tgaaggttat atccaggggg
      841 caggttcagc aggtccccgc acaaagtcaa aaccgcggcc ccggcaactc aacgggtgat
      901 ggttaaaaaa ttaaacaggt gaaggagaat aatatgcgtt tagcatcccg atttggactt
      961 actcattcca ttcgtcagga acgcccactg actaacgatg aattagtcaa ggttgtacct
     1021 tctgtgtttt cagaagaaaa gcataattca cgtagtgatc gctataccta tattccaacc
     1081 attacactgc tggataaatt acgtgaagaa ggtttccagc cattttttgc ctgtcagtca
     1141 cgagtaaggg atgaggataa acgggggcat actaagcata tggtaagact tcgccgtgaa
     1201 ggggccaata aaggaacaga agtacccgaa atcattcttc ttaactcgca tgatggatcg
     1261 tccagttatc aaatgatccc gggtatgttc cgttttgtat gcacaaatgg ccttgtgtgc
     1321 ggtacgtcgt ttggtgagat tcgtgtacct cataaaggtg atattgttgg aagggttatc
     1381 gaaggtgctt acgaagttct gggaattttc gataaaataa ctgaaggcgt tgatgtcatg
     1441 aagtctattg ccttaaccaa ggaagaacag cgtctgtttg gacaagctgc attaacctac
     1501 cgatatgaag atgaaaataa atctccggtc agtattgaac agataattca tccgcgccgt
     1561 tatgaagata aaaaagatga catctggact acatatcaaa gggtccagga aaatctgatt
     1621 aaaggcggtt tgcctggccg aactgaaaaa gggaaaagaa caacaacccg gccagtaaaa
     1681 gcaatcgatg gtgatgttaa actcaataag gcattgtggt taattgcgga gaaattccgc
     1741 actcttaaag gctaattata agcgccgggc agctgtccgg cgttttgatg gtgggaatac
     1801 ttatgtttga atttaaagct gtctgtatca gtatttcaca tatggaagaa ggtgatatca
     1861 gagttcttca ggtgctttca tcaacaacca attatctaac ttgcgatagt tacttagagt
     1921 ggatacatga tacaccttat ggttgtgtta taaggatgta tgatttcaaa cgccatcttc
     1981 tggcgctaaa aagaggtggg gtgtcaaaat ttttacgcaa aactcttata ctaatgcacc
     2041 gggagggtgg gtatctgctt tttcattttg atagtgatgc cgattattat ccaggtatag
     2101 atgccgtaga tttttaagaa attcaagcct tcttatatca gaaggctttt tagttcatgg
     2161 cgattttctg atctttagaa gaagttcgtt tgcttgcttt gatagccgtg tagtagtaat
     2221 ggatcttctt cgcatactcg taacgtcttg ctgcctggcg cggggtcttt ttgaagcctg
     2281 cattgtatgc cccgactgca tcccacgaaa cgccccactt tttaaacgcc tgagccagat
     2341 aataggctcc ggtgtagatg ttaagacaag catcgctaag aagatgatcc ggttttattc
     2401 catatcgcga cagttcgttg aaatgctgcg agtcaatttg catcaagcct gatccataac
     2461 ccgtaacagg attacgccca atcgcatcaa ttttaaatcc tgattcttgc catgatatag
     2521 cacgcaggag atccggatca attttgtaat ccctgccagc catatcaaag cagtctgctg
     2581 ccattgatga caatggtatt aaagacagga tgataccaaa cgctactccc ttcatgaatt
     2641 cccctccgtg ccaaatcccg gaataacctt aacccatcca agaccgagac tcaacatata
     2701 tgcactcctc accacaaaat caccacacac tacgcaaaat taaaattttg cagatttcac
     2761 tttagcttca gtggcttaac tgtagttata ttacattatg ttatttttct ttatatatat
     2821 gtgagttgcc ggtgagtcag tagtaagtcg tcggtaagtc tatagcgact cgtttatgac
     2881 tcactctttt ataatcatat atttaaagcg acttaactct agacttaagt cgccatatga
     2941 ctcgtaatgc aactttctta gactctagac ttaagtttag agttgtaaag aaggtaaact
     3001 cgcgttatct tctcgccata ataacttgtt tgatgctgtt tttaataaag gaaccgatat
     3061 ggccagggta caagcttatg ctagtgatga agttgcagag aaaattaatg caatagtgga
     3121 aaagcgaagg gctgaagggg caaaggaaaa ggatgtcagt ttttcaagtg tcgcttcgat
     3181 gctccttgaa ttagggctta gggtttatga ggctcaaatg gaaagaaaag attcagggtt
     3241 taaccaaact gagtttaata agattttgct tgagaatgtc atgaagactc agtttaccgt
     3301 atccaaactt ttggctattg gctctctaag ccctcatgtt acaggtgatg aacgattcga
     3361 gttccgcagc atggtgagta acattcgaga agatgcaaaa gatgttattt ctcacttctt
     3421 ccccgagcag gaagaagagt aagaactggt gtgtgtttat ggtggtttgt aattaattca
     3481 gcagtaaaaa aagaataatg atctagcttt aaaaagtgac atggaagtat aaggaatata
     3541 gaatacttac agggttgtac taaaatttgt tgaagcgttg acgtccgggg cttgtccccc
     3601 cgaacgtcaa ctatccatag tgtcctttcc aggaggcgcc tatgtgtgcg aaggatcgta
     3661 gtcatattgc tgtgcgcagt caaacagatt ccattcattc tatgcgcgag atgcttaata
     3721 tttcatcttg ccctgctttt attcgtgatt cccggggcga agtaattcac accagcccaa
     3781 cctttgacaa aattttctta acatctggtg gaactggagc atttgataca cccagagcac
     3841 cgggaagctg gtttctggat ctctccctgg agatcaaact ggaactgatg cagtcagaac
     3901 tgaaatcgtt ttcagaaggt tctgctgtac ttgttaaaaa tatttggttg gcaggttcac
     3961 tttggactgt ttttattgaa acgttctcga ttaatgaaga tacatacagt aaatgggttt
     4021 ttataaatga agatgaaccg atacctcatc cttcaatgga atacaatgac ttttctataa
     4081 agatgcaaag atatatcgaa agaattcagc gttcagcaaa gtctgactgg gctatcttta
     4141 atctgtatgc ggttgggttt tctcatgcat ctatttctaa gattacaggt gttggggtgc
     4201 aaacctcaaa aaatacagtt agtaaaataa aaaaggaact gagctttgat gatcgtgact
     4261 atatcataat gtcatcgatc tatactctaa gttatggaaa gtttatatcg aatgtggttt
     4321 cgatattaaa agacggtgtt aattttttgt taaatcaaaa gtgccatcgg tacttttgat
     4381 ttgtgaaaat aatttactca agatttctcc ttagtttgtt tacttttaaa tcctccaatg
     4441 taagatttat taaaggttgg taatcttact ttggaggtcg attggaaaga gcgattagga
     4501 gcaacgcacg acctgcgcct ggaaaaaatg tgcttttaaa attagatgaa gaaactaatc
     4561 gtcgactcat caaagcaaaa gacagaagca gaagaagtaa aacagctgaa gcttatttga
     4621 gactgaaaga tcatctagaa cgttttccgg acttttataa ctcggaacta gccgtaccag
     4681 gaggtgaaaa ggaagagtga tttaacatca tagtcaaaaa tctgttttat atatcaacca
     4741 ttctttttaa aggaaataaa tatgaatgct gtattaagtg ttcagggctc tgctgtctct
     4801 gaaaaaacca aaccgtcttt tatgtcccgc gttttcaata aaaagaaagc cctgaaagtc
     4861 gctaaagttg cgctgccggt tgctgctgtg gcggcgttct tcccggatgc tgcgatggcc
     4921 accaccacgg caaccgacct gatgaagtcc ggggatgcga ccgttaaagg cacgtttggt
     4981 aagcagtcca gcgtcgtcaa atgggtggtt ctggcagaag ttgtcgccgg cggcatcatg
     5041 tacatgatga ccaaaaacgt gaagttcctg tttggcttcg cgattatttc cacctttatc
     5101 accatcggta tgtcggtcgc cggttactga caggaagcac gtgatgacgg gagacgaatt
     5161 aaagcgatac cgtttcccgg aaacactgac taaccagtcc cgctggtttg gcttatatct
     5221 cgatgaactt atcccggccg tgatttgtct cggctggggt ttctggacca gcaagttcat
     5281 ttttggcata gcttcagcgg tactggtttt ctggggtatc aagaaactga aaaaagggcg
     5341 gggcagttcc tggctacgcg acctgattta ctggtatctg ccaacctccc ttcttaaagg
     5401 tttctttcat gatgttccgg actcctgttt ccggcagtgg attaaataaa tcaacgggga
     5461 aataacatat ggaacatggt gccagacaaa ataccacccg cgttatcgct gtgggattta
     5521 tcagtctggg gacactcctg accctcagtc ttgcgaccaa tattatccag ggcataaata
     5581 attaccgtct gcagacggaa cagaaagtgg cggtgacacc gatgttattc cgtgcaccct
     5641 ttgcggtgtc tcagaaccag gctgacgcct cttatattga gcagctgggc ctgtcctttg
     5701 ttgccctgcg tctgaatgtc acgccggaaa cggttgatgc ccagcatcag cagctactcc
     5761 ggtatgtatt acccgcctcg cagaacagcc tcaaggtcca gctggctgaa gatgcgaaac
     5821 gcattaaaga caataaggca ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt
     5881 caaaggcctt acatttcaaa aactctgctt accaggcgca tttcgcccag gggatcacca
     5941 taataaaatg ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat acctttgatg
     6001 gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg cgccgattat ccgtttcagt
     6061 ttgccatgat cgcattcaat cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca
     6121 gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc gggcgcttta
     6181 tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt tgttgaggat tttacccaga
     6241 aaccggtatg cagctttgct gttacgacgg gaggagagat aaaaatcgac agtgcggccc
     6301 cggctgtcga cggcccggta cagatacgcc cagcggccat tgaccttcac gtaggtttca
     6361 tccatgtgcc acgggcaaag atcggaaggg ttacgccagt accagcgcag ccgtttttcc
     6421 atttcaggcg cataacgctg aacccagcgg taaatcgtgg agtgatcgac attcactccg
     6481 cgttcagcca gcatctcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt
     6541 acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc
     6601 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgcccggg
     6661 aaactgcgct gccggagagt gtgctgcgtg aaccacaacg ggagcgggaa gcggtccggg
     6721 aggttgcccg ggaaaatctg ctgcaggagc gtctgcagca gatggagcgg gatatggtcc
     6781 gtgacctgca gaaagagaaa accctgggcg gagactgata caggaaggca aacgctgatg
     6841 acaacggata acacgaacac gacacgtaac gattcactgg ctgcccggac cgatacctgg
     6901 ttgcagtcgt tgctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg
     6961 gtgctgatgg tactggacca catcaatctg atattccagc tgaagcagga atggatgttt
     7021 ctggccgggc gtggagcctt tccgctgttt gccctggtgt gggggctgaa tctgtcccgt
     7081 catgcgcata tccggcaacc ggccattaac cggctgtggg gatgggggat tattgcccag
     7141 ttcgcatatt atctggcagg ctttccctgg tatgagggga atatcctgtt tgcctttgca
     7201 gtggcggcac aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc
     7261 attcttctga tggcgctgtg ggggcctttg tccggcacca gttacggcat tgccgggctg
     7321 ctgatgctgg cagtcagcca ccggctgtac cgggcggaag acagagcgga acgtctggcg
     7381 ctggtcgcct gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgcagcg
     7441 gcggtggccg gtcttgtgat gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa
     7501 tcattacccc gtttctggcc cggggatttt ttcccggtat tctacgcctg tcatctggct
     7561 gtgctgggcg ttctggcgct gtgacgggtg tggtatcttt gaccgcaaga ggatgattcg
     7621 tcagaggcag aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaaa
     7681 acggaagggg agacgcctgt ccgcaggcgg aaaaccatca tcaatgtcac cacgccacca
     7741 aagtggaagg tgaaaaagca gaagctggcc gagaaagcag cccgggaagc agagctggcg
     7801 gcaaaaaaag cgcaggccag acaggcactg tccatttatc tgaatctgcc cacgctggat
     7861 gaggccgtga ataccctgaa accctggtgg ccgggattgt ttgacggtga cacgccccgg
     7921 cttctggcct gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg
     7981 ctctcgcata aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg
     8041 tgtgccatga aagccggtgc ctgccggtat gacacggaag ggtatgtgac ggagcatatt
     8101 tctcaggagg aggaagcgta tgcggcagcg cgtctggata aaatccgccg ccagaaccgg
     8161 ataaaggcag aacttcaggc cgtgcttgat gagaaataaa aaaagcctcc atcagagagg
     8221 aggcagggaa ataaggctta aaaggaaata acttccgtac ccgaaaggaa gccaattcag
     8281 tagattattt aacaccataa tactgacggg ttaaagagga gtgataaaga tgaatggatt
     8341 cagaaacagt tcacggaacg gtcaggtctg gcgctggcag cgtgccggaa gccgtgccgt
     8401 cagcctggag gtcaggggat tccggaagga ggccgctgaa gcgtggctcc tggctgcccg
     8461 tgttgctccc cgtacagact ggcagcagtt tgcacggcag agagccgaac gctgtcagcg
     8521 acggagataa tgaaaaaaaa cggcaaaaaa aagacttccc ctgagcaaag gaggccaaaa
     8581 atcattcata gcgtattact gacattgaat gaatgctctg tacatcaacc ctgtgaagct
     8641 accaccggtt aatgccggga ggttgcggga agaaagataa tacacagtca ttacgtggtt
     8701 aatatctgtc agaatgtttt ttgtgaccat aaactgtcag atggataaaa aagaccgtca
     8761 tgtgacggtc agtgttgtcc tgtcagggac aaatgcattc agtaaggaga gcaaaataat
     8821 aacatcacgt tgcccggctg ttaatatcag gcgatgttaa gtgttattta tgactctttt
     8881 atctggttgt gaagtctgtt aatcaggaag agaaaatggc gaccggaaaa tcagagaagc
     8941 aacaggtgat tgcctttatc gattacgaga actgcagtaa cctgacagaa atttccttaa
     9001 atatatatac ggagttaatt atattcgcgg gtgcgaaaca gaataatgtg gcactgccag
     9061 taaacgcttt ccctgaagca gtaaaaatca cactccggca tgtcagtgaa gtctcccgga
     9121 acaatgttga ttttcatctg gtgctggagc tggggcagtg tgtatgccac tacgggccgg
     9181 agaaggaata ccatattgtt tccgctgaca agggatatga cggtattgtc cggacactgc
     9241 aggcgcgcgg gattcgctgc cgtcgcgtct ccccggataa ggcgtcatca gtaaagatgg
     9301 ctgcacccga catggatatc gttatccggt gggcaaacaa aattcaacag gcagcgcggg
     9361 aggttcgtaa tatgccggta acagaaaaga catttaataa ttatttaaaa tcacagatgc
     9421 gcggtgaatg gcgtgatgtc ctgaccagag gtatccggga tgaactggtc cgtcgcgggg
     9481 taatgaaaat tcaggggaat aaaatcatct ggtaagactg ccagaataaa atgatattta
     9541 ttttatttat aaaaacagaa aggtcgaaaa tcaataatgg catttatttc catgacaaga
     9601 cagaaagcgc cgtacagata tgcaagcaag acccggttat cgataagacg aacagactca
     9661 ggctctgccg ggtgttatgt gacggttgcc gttcggggga agcaggaatt tattattgct
     9721 gaacttgatt cagaaatccg gaaaatcagg ctgaaactga cggactccta tgaagaaggg
     9781 gtaaggctgt cttcgggcac gtttacgctt ccggccagat tctgccggga aatactgccg
     9841 gacgacgtca ggagtatcac cattattctg gaaaaaagcg acgatgagtg gtggtacggg
     9901 agttactgat atgtggcaga actccggaca ctgacgcctt cgcgttgctc agttgtccaa
     9961 ccccggaaac gggaaaaagc aagttttccc cgctcccggc gtttctgcaa taaaaagcag
    10021 gggcatggag gtcgtcgtaa ccagacagga atgtggtatt atcgcggcgg gtgtctgagc
    10081 ctttctggtt caggcaagac gcaggtacca gaaatgcgaa gaccccactc gttaatccat
    10141 taactcgtga ggtctgcatg aagtacttag cactactgat tgtagcctct tctttgcaga
    10201 gaggtcaaag tttatgacga aatatgccct tatcgggttg ctcgccgtgt gtgccacggt
    10261 attgtgtttt tcactgatat tcagggaacg gttatgtgaa ctgaatattc acaggggaaa
    10321 tacagtggtg caggtaactc tggcctacga agcacggaag taagctgccg ggcggggacg
    10381 gaagtccccg ctttccggaa gtgtgaggta tttcaggggc agacacccga catgccagaa
    10441 acagccggtc ccgcccgggg ccggcatcca ggttaaggca tttcctgttt ttcagtcatt
    10501 tcatcatcaa aatcacatta aacggtcgta atcagacatg atttgtgcgc caacacagat
    10561 catcatcaca actttcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat
    10621 ccctgtttga gaattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat
    10681 caggtacaaa gcgagcatac cgaaaaggta atcctctgac gcttgcagaa cgtcagcagg
    10741 catctctggc aagaaaacgt gccacacata aagaactcag agtgttcatt cctgctgcgt
    10801 taaaagcgca attacaggag atgtgtgacg ctgaaggtgt tactcaggct gagatgattg
    10861 ctgaactgat aaagcagaag agtgctttca gctaactaaa atgtagcgac atcacattct
    10921 tgcctgtttg tggtgtgcgt gatagattgc tgatcgttta aggaattttg tggctggcca
    10981 cgccataagg tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcgaaa
    11041 accccgataa tcttctttaa ctttggcgag tcagaaagat taccggggct aacaagaaac
    11101 tgcatagaag ctgttgctct atgcggggag tatagttata tgcccggaaa agttcaagac
    11161 ttctttctgt gctcactcct tctgtgcaac ataagtgcag gatggtgtga ctgatcttca
    11221 acaaacttat taccgccagg taaagaaccc gaatccggtg tttacacccc gtgaaggtgc
    11281 cggaacgctg aagttttgcg aaaaactgat ggaaaaggcg gtaggcttca cctcccgttt
    11341 tgatttcgcc attcatgtgg cacatgcccg ttcgcgtggt ctgcgtcggc gcatgccacc
    11401 ggtactgcgt cgtcgggcta ttgatgcgct gctgcagggg ctctgttttc actatgatcc
    11461 gctggccaac cgtgtccagt gctccatcac cacgctggcc attgagtgtg gactggcgac
    11521 ggagtctgat gccggagtac tctccatcac ccgtgccacc cgtgccctga cgtccctggc
    11581 tgagctgggt ctgattacct accagacgga atatgacccg cttattggtt gcaacattcc
    11641 gaccgatatc acgttcacac cggcgctgtt tgccgctctc gatgtgtctg aggtggcggt
    11701 ggcttccgca cgccgcagcc gtgtggaatg ggaaaacaga caacgcaaaa agcaggggct
    11761 ggataccctg ggcatggatg aactgatagc gaaagcctgg cgttttgttc gtgagcgttt
    11821 ccgcagttac cagacagagc ttaagtcccg gggaataaag cgtgcccgtg cgcgtcgtga
    11881 tgcggacagg gaacgtcagg atattatcac cctggtgaaa cggcagctga cgcgcgaaat
    11941 cgcggaaggg cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt
    12001 gaaggagcgc atgattctgt cacgtaaccg caattacagc cggctggcca cagcttcccc
    12061 ctgaaagtga cctcttctga ataatccggc ccgaaccgga ggcattcgca cgcctgaagc
    12121 ctgccagcga acaaaaaatc agcaccacat acaaaaaaca acctcactat ccaccttctg
    12181 gagcatccgg ttccccctgt ttttaataca aaatgcgcct cagcgacggg gaattttgct
    12241 tatccacatt taactgcaag ggacttcccc ataaggttac aaccgttcat gtcataaagc
    12301 gccagccgcc agtcttacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt
    12361 aaactactta attacattca tttaaaaaga aaacctattc actgcctgtc ctgtggacag
    12421 acagatatgc acctcccacc gcaagcggcg ggccccgacc ggagccactt tagttacaac
    12481 acacaaaaac aacctccaga aaaaccccgg tccagcgcag aaccgaaacc acaaagcccc
    12541 tccctcataa ctgaaaagcg gccccgcccc ggcccaaagg gccggaacag agtcgctttt
    12601 aattatgaat gttgtaacta catcttcatc gctgtcagtc ttctcgctgg aagttctcag
    12661 tacacgctcg taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt
    12721 aaaccctcgt cgggaccact ccgaccgcgc acagaagctc tctcatggct gaaagcgggt
    12781 atggtctggc agggctgggg atgggtaagg tgaaatctat caatcagtac cggcttacgc
    12841 cgggcttcgg cggttttact cctgtatcat atgaaacaac agagtgccgc cttccatgcc
    12901 gctgatgcgg catatcctgg taacgatatc tgaattgtta tacatgtgta tatacgtggt
    12961 aatgacaaaa ataggacaag ttaaaaattt acaggcgatg caatgattca aacacgtaat
    13021 caatatctgc agtttatgct ggttatgctg gctgcatggg gcattagttg gggagccaga
    13081 tttgtcatgg agcaggccgt tctgctttat ggatcaggaa aaaactattt gttcttcagt
    13141 catggtactg ttctgatgta cctgctgtgt gttttcctgg tataccgccg ttggatagct
    13201 ccgctaccgg tcgttggtca gctgcgcaac gttggcgtac cgtggctggt cggtgcgatg
    13261 gccgtggtgt atgtcggtgt atttctgctc ggtaaggcgc tggctctgcc tgctgagcca
    13321 tttatgacga aactttttgc cgataagtcc atacctgacg tgatcctgac gttgctgacc
    13381 atctttatcc ttgccccgtt gaatgaggaa acgctgttcc gggggattat gctgaacgtc
    13441 ttccgttcac ggtactgctg gacgatgtgg ctgggggcgc tgataacgtc gttgttgttc
    13501 gtcgccgcgc acagccagta tcagaacctg ctgacactgg cagaactgtt cctggtgggg
    13561 ttgattacat cagtggccag gatcagaagt ggtggcctgc tgctgccggt attgctgcat
    13621 atggaagcaa ccacgctggg tttactgttt ggttgaaagt tatattttta ttaaacattg
    13681 tgcgttaaag cctggtgtgt ttttttagtg gatgttatat ttaaatataa cttttatgga
    13741 ggtgaagaat gcataccacc cgactgaaga gggttggcgg ctcagttatg ctgaccgtcc
    13801 caccggcact gctgaatgcg ctgtctctgg gcacagataa tgaagttggc atggtcattg
    13861 ataatggccg gctgattgtt gagccgtaca gacgcccgca atattcactg gctgagctac
    13921 tggcacagtg tgatccgaat gctgaaatat cagctgaaga acgagaatgg ctggatgcac
    13981 cggcgactgg tcaggaggaa atctgacatg gaaagagggg aaatctggct tgtctcgctt
    14041 gatcctaccg caggtcatga gcagcaggga acgcggccgg tgctgattgt cacaccggcg
    14101 gcctttaatc gcgtgacccg cctgcctgtt gttgtgcccg taaccagcgg aggcaatttt
    14161 gcccgcactg ccggctttgc ggtgtcgttg gatggtgttg gcatacgtac cacaggtgtt
    14221 gtacgttgcg atcaaccccg gacaattgat atgaaagcac ggggcggaaa acgactcgaa
    14281 cgggttccgg agactatcat gaacgaagtt cttggccgcc tgtccactat tctgacttga
    14341 acatggggtt tgaggggcaa ctggatgaaa acgtacgatt tagggcacta aaaccgctgt
    14401 tgtcccacca ttctggtgat tcccaaacgt tatttggcta aaaagtagtt ttgatgtggt
    14461 ttattttccg aattccaagc gcagccctac tttcttgtgc gtatttgcgt gttttgcgca
    14521 gttttgaagt tccggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
    14581 gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg
    14641 tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac tgccgtaaaa
    14701 catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg
    14761 atatggccat gaatggcgtt ggatgccggg caaccgcccg cattatgggc gttggcctca
    14821 acacgatttt ccgccattta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc
    14881 gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggatacg tcggggctaa
    14941 atcgcgccag cgctggctgt tttacgcgta tgacaggctc cggaagacgg ttgttgcgca
    15001 cgtattgggg tctgacgctc agtggaacga aaactcacgt taagcaacgt tttctgcctc
    15061 tgacgcctct tttaatggtc tcagatgtcc tttggtcacc agttctgcca gcgtgaagga
    15121 ataatggccg agcatattga tatgtccgtg gcaaagcggg gagaggcgtg cgatatcttc
    15181 atcattcagt gtttcaccct gcgcccggag atgatccagg gctgcctgca tataaatagt
    15241 gttccataac acgacggcgt tagtgaccag ccccagtgcg cccagttgat cttcctgacc
    15301 gtcggtatat cgttttctta tctcaccttt ttgaccgtga cagatggctc tggcaacggc
    15361 atggcgactt tctccccgat taagctgggt cagaatgcgc cggcggtaat cttcatcatc
    15421 aatataatta agcagataca gcgttttgtt gatgcgcccc acttcaatga ttgcctgagt
    15481 cagtccggaa ggacgttcac ttttcagcaa tgaacggacc agcactgaag cctgtacttt
    15541 gcccagcttc agggagccag cggtccggat catttcgtcc cactgaagga ctattttt
//
