LOCUS       NZ_CP130269             5596 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Klebsiella pneumoniae strain DX3-2hy plasmid p5, complete sequence.
ACCESSION   NZ_CP130269
VERSION     NZ_CP130269.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36411140
            Assembly: GCF_030506115.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yang,Y.
  TITLE     Genomic of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) College of Basic Medicine, Zhejiang Chinese
            Medical University, Puyan, Hangzhou, Zhejiang 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JUL-2023
            Assembly Method                   :: SPAdes v. 3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/19/2023 18:01:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,810
            CDSs (total)                      :: 5,690
            Genes (coding)                    :: 5,522
            CDSs (with protein)               :: 5,522
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 65 of 168
            Pseudo Genes (incomplete)         :: 109 of 168
            Pseudo Genes (internal stop)      :: 24 of 168
            Pseudo Genes (multiple problems)  :: 30 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130269.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DX3-2hy"
                     /isolation_source="feces"
                     /host="elephant"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019-12-09"
     gene            33..542
                     /locus_tag="Q2T50_RS29005"
                     /old_locus_tag="Q2T50_29010"
     CDS             33..542
                     /locus_tag="Q2T50_RS29005"
                     /old_locus_tag="Q2T50_29010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            549..761
                     /locus_tag="Q2T50_RS29010"
                     /old_locus_tag="Q2T50_29015"
     CDS             549..761
                     /locus_tag="Q2T50_RS29010"
                     /old_locus_tag="Q2T50_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1428..1652
                     /locus_tag="Q2T50_RS29015"
                     /old_locus_tag="Q2T50_29020"
     CDS             1428..1652
                     /locus_tag="Q2T50_RS29015"
                     /old_locus_tag="Q2T50_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1636..1902
                     /locus_tag="Q2T50_RS29020"
                     /old_locus_tag="Q2T50_29025"
     CDS             1636..1902
                     /locus_tag="Q2T50_RS29020"
                     /old_locus_tag="Q2T50_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1972..2322)
                     /locus_tag="Q2T50_RS29025"
                     /old_locus_tag="Q2T50_29030"
     CDS             complement(1972..2322)
                     /locus_tag="Q2T50_RS29025"
                     /old_locus_tag="Q2T50_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2388..2933)
                     /locus_tag="Q2T50_RS29030"
                     /old_locus_tag="Q2T50_29035"
     CDS             complement(2388..2933)
                     /locus_tag="Q2T50_RS29030"
                     /old_locus_tag="Q2T50_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2959..3285)
                     /locus_tag="Q2T50_RS29035"
                     /old_locus_tag="Q2T50_29040"
     CDS             complement(2959..3285)
                     /locus_tag="Q2T50_RS29035"
                     /old_locus_tag="Q2T50_29040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4441..4617
                     /locus_tag="Q2T50_RS29040"
                     /old_locus_tag="Q2T50_29045"
     CDS             4441..4617
                     /locus_tag="Q2T50_RS29040"
                     /old_locus_tag="Q2T50_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4607..4951
                     /locus_tag="Q2T50_RS29045"
                     /old_locus_tag="Q2T50_29050"
     CDS             4607..4951
                     /locus_tag="Q2T50_RS29045"
                     /old_locus_tag="Q2T50_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5133..5378)
                     /locus_tag="Q2T50_RS29050"
                     /old_locus_tag="Q2T50_29055"
     CDS             complement(5133..5378)
                     /locus_tag="Q2T50_RS29050"
                     /old_locus_tag="Q2T50_29055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4276..4355
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3693..4090)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..877
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.658"
                     /coverage="99.0"
                     /evalue="1.0900000000000001e-167"
     Typing          4942..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.69e-139"
ORIGIN
        1 agggcgaaac atccgactta agggagccat ctatgaacag tcttttaacg ctggcgaagg
       61 acttagagca gaaatcgaaa gcgcagcagc agagtaccgg cgaaatgctg aaagccgcat
      121 tcagcgagca cgagaagtct gtcagagcgg aactgagcga aagcgggaag agaatcagcg
      181 ccgccatccg cgcccacgag cagggtatga gcgcggccat gcagtcgaac cgcctgagcg
      241 tgatgcgtat ggtcgggcgg acatggctga ccatcacgat ggtgtcagga ctgctgttcg
      301 ccagcctgag cggggtgttg tggtatcagg ggagcttgat agcgtcaaat ctggccgaaa
      361 tcgaccggca gaacgcagcg ctgtcgaagc tgaacgcgaa gacctggggc gtgacgtacc
      421 tggaagacag caacggacgt tttctggtgc tgccgaaggg gacggccgta gacaggacgc
      481 agagctggac ggtcgggaac gggcggtcga agcagaacgc gctgagactg gtgaaggagt
      541 aagtgccgat gaccgagctg gaaaaacagt tgctgagcgc attagagcag ctacagcagg
      601 actactcgaa aaggctggac gagtgggaga gcgccttcgg ggaatggcgg aaaatgtcag
      661 ggcttatgca acgggagaac gcggcgctga gcgagcgcgt gacgcgcttg agtcagcagg
      721 tggaacgctt gagcgggcaa ctgcagcgtt tgagccggta gtgcagcgtc atgagatggc
      781 cgtggccgca gaacgggcgc acgagcaacg gcagcacgaa aaggaactga cggaagcgcg
      841 ttcccggcag cgaagctatg acggtccgtc gctgggctga aatgcaggaa aacagggttc
      901 tcatcgggca tttacgctga aaccggatgt cattcacctg gctgcatggc tatgcagcca
      961 ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt ggtttgttgc cggttttacc
     1021 tgactgccac cccgttcgcg gcgaacccgt ccggggcggt gcgggcaacg ggtgttatgt
     1081 ttaatcagcg tgaacggcag atacaaaaaa gccccggagg gcttttaact actgacttat
     1141 ttttttgaag atttgctggg tttcaatttg tcagtatttc ccggcgtttt tttattgtca
     1201 cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc gtttcggacc cggcttaaga
     1261 cccataatcg ttatcctttc aatagttaag aggaaagccc tcaagctaga tatattgacc
     1321 gcgttacgga ttatcaagag gggctaacaa gattattttt tgttctttta ggttgattgt
     1381 ggtactgttg atatacatgt atagcaaatg gtgttcggga ggtttatatg cttgctatcc
     1441 ggttatctga tgagattgag tcccgtctgg actcgctggc gaagcaaacc ggcagaacaa
     1501 agacgtttta tgcgcgggaa gcaatactgg cgcatctgga agacctggag gattattatc
     1561 tttcagcaga aactgctgca cgcgttcgcc gtggtgatga agcagtgcat tcgtctgaag
     1621 acgtgaggaa gtcacttggt ctggacgatt aactattccg atcgggcgct caaatcgtta
     1681 cgcaagatgg acaaacagaa cgcacgacgg attgtggatt ttatgagttt acgcattgca
     1741 gttgctgccg atcctcgcca gtcagggaag ccgctcaaag gtgagctggg cgagttctgg
     1801 cgctatcggg tgggagatta tcgcgttctg tgtgagatcc gagatgacga gcttgttatc
     1861 cttgccgcca cgattggaca tcgccgcgaa gtttacgact gaagcccgct atgcgggctt
     1921 ttttgatgcc gacaacaggt attatggtaa atactcgact aactacgtgt tctactgttt
     1981 gtcggtttta gttaaccagc aggttgtctt aacgataatg tagccgttat ttttaactaa
     2041 taaggtcata ttgttaacgt caggcataat aaaagacatt atggcctcag agttatcatc
     2101 agaagcagct ctctgcatta ctacttcgat tcccttttct ttgaatctat aagtatccag
     2161 ttgcctatta aacttagaaa gacctacaac catacttagt ttgttatctt taacagtaag
     2221 agatgcttcc tgagtaccat tacatcggta gtaagcggtg taaccgtcag caaaagatcg
     2281 accggggaat ataaatgcta ggaacgcaat taaccaatac atagttgaga atcttggtat
     2341 tacacatttc actatcctct ctttcatttt tcgttcacca tactttgtta tctgattttt
     2401 aatttttcta ttttataaac caagtctcta ccaaaaaggc gaaaattatc ctcaaataat
     2461 aataattgtt taagaacatc catgaaagca gctgcttcac cctcagttat gggagctact
     2521 ctatataaca tataacctga gttaagggtt tcttttatac tttcttcaat gctcattata
     2581 aatattagtg gattatcatt tttcttcccc catgggtttc taattaatcc ttcgatgtac
     2641 tcaggtcctt tgcgtcctat tgcctgcgca atatttttta tttcatctgc gtgagggctt
     2701 tgttgtacag ctttgtttat gcctgcttga gtgattttca gagactctac accgtgtatg
     2761 ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa ttaatccatt tttgtttaca
     2821 tctgagtatt tggataaaaa acgtaatgaa gtgttaactc tcccttgaag cctaacaatt
     2881 gggagtgctg ccttgtatat ttccttctcc gttaaatacc gtttaaaaaa cataattctc
     2941 cgcctccgta tagcgtgcct attttatttc gtcgtaaaac ttagctctga aatattcctt
     3001 tagaaccatc atgtcattaa taattgaatc tatctcatcc attttttctt ggtattcttt
     3061 taatggtgaa gggagcggta actcaatatt tgaaaagtga ttggcgagat agggatcaag
     3121 gtttctcatc cattcaggct tttcgccaat gcgttcttca actgaaagtg aacctgataa
     3181 tctctggtta ataagatcac ttagactcat tttggtttta ttcgaaagcc gttgtaagga
     3241 ttgctttaat cctatatcta gcatgatatt tgttctttct ctcattaccc ccccttgcta
     3301 ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta agaaacgcaa taaaaaagga
     3361 gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg gcatcagtgg cgggtgtttt
     3421 tccgtgggat ggatggagct ccggggataa cagaactgac gatcatttaa aggatcttct
     3481 tgagatcctt tttttctgcg cgtaatctct tgcactgtaa acgaaaaaac cgcctgggga
     3541 ggcggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgggtaa ctgggtatac
     3601 gagaccgcag ataccaaaac tgttctttca gtgtagccgc agttaggcac ccacttcaag
     3661 acctctcaat atctctctcg tatatatcca gttaccaatg gctgctgcca gtggcgtttt
     3721 tgcgtgtccg tccgggttgg actcaagatg atagttaccg gaaagggcgc agcagtcggg
     3781 ctgaacgggg ggttcgtgca tacagcccag cttggagcga acgacctaaa ccgagccgag
     3841 ataccagcag cgtgagctat gagaaagcgc cacgcttccc gaaagggaaa aaggcggaac
     3901 aggtatccgg taaacggcag ggtcggaaca ggagagcgca cgagggagcc gccggagaga
     3961 aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc tctgacttga gcgtccattt
     4021 ctgtgatgct cgtcagggga ggcggagcct atggaaaaac ggcaacgccg catccttttc
     4081 tccggccctt tctgatttga gcgcctttgc tttatcccgt tatccgtgag tcggtcacgc
     4141 ccgccgcagc cgaacggcag gagcgcagct accgagtgag cgaggaggcg tcatttcatc
     4201 tgtccgtgca tggtattgcg gacgctcctg agccacatat cacacctgcc acatgaggca
     4261 ctttcatgaa tcgtgctctc atgccaacat agtaagccag tatacacagc gtaaacgctg
     4321 tgactggttc agggctgcgc cccgaaaccc gctaacacca ctgacgcgct ggcgcttgtc
     4381 caacgccgga gcgacccgga aaacttttcc gtgccgtccg gcgtttgcga aggagggata
     4441 atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa gatcctggca tccggcagtg
     4501 atggcaaatc tgatgcgtca ggcacaggag gaagattacc gccgggagca gatggcagag
     4561 cttgagcgtt cagcccggtc acattttgcg gatatcggag gagaggatgg cggttaagcg
     4621 tacccgtttt ttaggtatcc gggtgaccga tggggagtac cagcagctgc tggagcgctg
     4681 caatggaagg caactggcgg tgtggatgcg ggaaacctgt ctcgatacgc gcccggcgcg
     4741 gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc cagcttgccg ggatggggaa
     4801 caacctcaac cagatcgccc ggaaaattaa cggcggccag tggtcaggcg ctgaccgggt
     4861 tcaggtggtg gccgcgctga tggccattga tgccgaactc gagcggctgc ggcatgccgt
     4921 gctggaaaag ggggcagacg atgatcgtta agtttcatcc gcgagggcga ggcggcggtg
     4981 ccgggccggt ggattatctg ctggggaaag accgccagcg cgacggggcc atcgtcctgc
     5041 agggaaagcc ggaggaggtc cgggagctta tcgacgcctc gccctacgcc aaaaagtaca
     5101 cctccggcgt cctgtctttt gctgaacagg atttaccgcc cggccagcgt gaaaagctga
     5161 tggcgagctt cgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtgctgt
     5221 gggttgaaca ccgggacaag gggcggctgg aactgaattt tctggtaccg aacacggaac
     5281 tgctgaccgg caggcgtctc cagccgtact acgaccgggc agaccgtccg cgcatcgatg
     5341 cgtggcagac cgtggtgaac gggcgactgg ggctgcacga cccgaacgca ccggagaacc
     5401 ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa gcaggaagcc gcagaggcta
     5461 ttacgcgggg cttactggcc ctcgcctcgt caggggagct taaaacgcgt caggacgtca
     5521 ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac cacaaagagc agcatcagca
     5581 ttgccgaccc ggacgg
//
