LOCUS       NZ_CP130262            11972 bp    DNA     circular CON 21-JUL-2023
DEFINITION  Klebsiella pneumoniae strain DX2-3hy plasmid p4, complete sequence.
ACCESSION   NZ_CP130262
VERSION     NZ_CP130262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36410974
            Assembly: GCF_030506095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Yang,Y.
  TITLE     Genomic of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Yang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) College of Basic Medicine, Zhejiang Chinese
            Medical University, Puyan, Hangzhou, Zhejiang 310053, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 11-JUL-2023
            Assembly Method                   :: SPAdes v. 3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/19/2023 18:11:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,812
            CDSs (total)                      :: 5,691
            Genes (coding)                    :: 5,522
            CDSs (with protein)               :: 5,522
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 65 of 169
            Pseudo Genes (incomplete)         :: 110 of 169
            Pseudo Genes (internal stop)      :: 24 of 169
            Pseudo Genes (multiple problems)  :: 30 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DX2-3hy"
                     /isolation_source="feces"
                     /host="elephant"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2019-12-09"
     gene            complement(join(11258..11972,1..17))
                     /gene="mobC"
                     /locus_tag="Q2T51_RS28945"
                     /old_locus_tag="Q2T51_28945"
     CDS             complement(join(11258..11972,1..17))
                     /gene="mobC"
                     /locus_tag="Q2T51_RS28945"
                     /old_locus_tag="Q2T51_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(17..1981)
                     /locus_tag="Q2T51_RS28950"
                     /old_locus_tag="Q2T51_28950"
     CDS             complement(17..1981)
                     /locus_tag="Q2T51_RS28950"
                     /old_locus_tag="Q2T51_28950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3467..3616)
                     /locus_tag="Q2T51_RS28955"
                     /old_locus_tag="Q2T51_28955"
     CDS             complement(3467..3616)
                     /locus_tag="Q2T51_RS28955"
                     /old_locus_tag="Q2T51_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3701..3958)
                     /locus_tag="Q2T51_RS28960"
                     /old_locus_tag="Q2T51_28960"
     CDS             complement(3701..3958)
                     /locus_tag="Q2T51_RS28960"
                     /old_locus_tag="Q2T51_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3968..5653)
                     /locus_tag="Q2T51_RS28965"
                     /old_locus_tag="Q2T51_28965"
     CDS             complement(3968..5653)
                     /locus_tag="Q2T51_RS28965"
                     /old_locus_tag="Q2T51_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5758..7245)
                     /gene="ltrA"
                     /locus_tag="Q2T51_RS28970"
                     /old_locus_tag="Q2T51_28970"
     CDS             complement(5758..7245)
                     /gene="ltrA"
                     /locus_tag="Q2T51_RS28970"
                     /old_locus_tag="Q2T51_28970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7890..8135
                     /locus_tag="Q2T51_RS28975"
                     /old_locus_tag="Q2T51_28975"
     CDS             7890..8135
                     /locus_tag="Q2T51_RS28975"
                     /old_locus_tag="Q2T51_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8409..8780
                     /gene="tolA"
                     /locus_tag="Q2T51_RS28980"
                     /old_locus_tag="Q2T51_28980"
     CDS             8409..8780
                     /gene="tolA"
                     /locus_tag="Q2T51_RS28980"
                     /old_locus_tag="Q2T51_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8777..9142
                     /locus_tag="Q2T51_RS28985"
                     /old_locus_tag="Q2T51_28985"
     CDS             8777..9142
                     /locus_tag="Q2T51_RS28985"
                     /old_locus_tag="Q2T51_28985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9317..9595
                     /locus_tag="Q2T51_RS28990"
                     /old_locus_tag="Q2T51_28990"
     CDS             9317..9595
                     /locus_tag="Q2T51_RS28990"
                     /old_locus_tag="Q2T51_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9583..9897
                     /locus_tag="Q2T51_RS28995"
                     /old_locus_tag="Q2T51_28995"
     CDS             9583..9897
                     /locus_tag="Q2T51_RS28995"
                     /old_locus_tag="Q2T51_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10061..10489
                     /locus_tag="Q2T51_RS29000"
                     /old_locus_tag="Q2T51_29000"
     CDS             10061..10489
                     /locus_tag="Q2T51_RS29000"
                     /old_locus_tag="Q2T51_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10515..10694
                     /locus_tag="Q2T51_RS29005"
                     /old_locus_tag="Q2T51_29005"
     CDS             10515..10694
                     /locus_tag="Q2T51_RS29005"
                     /old_locus_tag="Q2T51_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10721..11251)
                     /locus_tag="Q2T51_RS29010"
                     /old_locus_tag="Q2T51_29010"
     CDS             complement(10721..11251)
                     /locus_tag="Q2T51_RS29010"
                     /old_locus_tag="Q2T51_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10653
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2784..3364
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.603"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11262..11971)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.093"
                     /coverage="98.0"
                     /evalue="1.28e-137"
ORIGIN
        1 tactgctcca gtgtcatcag taaagccccg caaaatcatc atccgctttt ggtccgccgc
       61 cgtcggccgg aggcaggtac tccgccagcg ggtccgtttt cgccagcacc gggaaggttt
      121 tcagcgaaat ttcccggcgc tcaacccgca gcgggctgct gaacgccagc cgcgccacgc
      181 ccagcccggt catcaccgcg aacccgtcag gcaggtgttg cagggtgttc acgtcaaaca
      241 gcggccgcgc ctccttctgg atgaagctgt cgccgctgat gtgctccacg ttccccgcct
      301 cccggctggc ccggcggcgc tccacgtcca cccggatctc cccggtctgg cccgccgccc
      361 actgcgcgga ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg ttatccagta
      421 ccgtggtttt cacaaaatca gcgggcagat cctgcccgca ctgaccgaag tcgcccagcg
      481 actggtgcgc gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc gcgttcagca
      541 cgtacttcga cagcagatat ttaatctcat cgagcatgat gctcgcgtgc ggccacctcc
      601 ggaactcatc ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc ttctgcaccc
      661 ggatcaccgc ctcatcgtcc atcgagccga tgacgtacag gcagccgccg ccgttgatta
      721 tccccgccac atcgataccg ctgtccgtct gcaggacgct caggtcggcc acgttctcca
      781 gcttggtgat cagccccttc acgtcctttt tcagggcctc cggcaacgcg tacgccgccg
      841 ccagcacctg ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc ttcgccgctt
      901 tctgctcgct gatgcggtag tggtccgcca cgtcgccggt ctcactcagg ttaaacccgg
      961 caaccagcag attcttcagg gcgtactggt cgagatcgcg gaacagattc agctgcgccg
     1021 gtttccccgg ccgcagatcc agcagggtga agtccgggca gtgcgcccgg aagacgtgcg
     1081 gcgcccacgc gtcccctttg gggtcgaagt acaccacagc gtccccgaaa tcgcgtacgc
     1141 agcgaatgag cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg ccgaggatct
     1201 gaatattggt cttgcgccag gtatcacgag ggaccagcac cggctggccc tgctcatcct
     1261 tacccagaaa caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg gccagcgcgt
     1321 taaccggtac cggctccccg aaatgcaggt ggcggacgtc tgtcatttct ccggtccgct
     1381 ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcggccgcaa
     1441 aatgaccatc aagcagcagg aacgccagca gccgcgccag aaggacgccg gcgacaagcc
     1501 ctccggcaca ccaggctatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt
     1561 tcgcatagag gtccgggcgc aggacggttt ctccccagct ctcgataaac acggcgacaa
     1621 ccagcccccc cagaagggcc agaccggcag cgccggtcag ccagtacgcg tagttccaga
     1681 catgtcgccg tgccctgacc atccgcccgg gtgaatccag cgaggccagc accgaaaatg
     1741 gcacagtccg gttattatcg cgcttatagc gatcggcata ctcccgccgc gccttatggc
     1801 tcagccccac aaaacgcatt cggccggccc agagttcagc cagaccgaaa aacaggccga
     1861 taaccagaaa aaaaccggcc agactgtaat actcccggta cagccagctg acccacgggt
     1921 aaatctgccg ggccagctcc gggatatgcc actgccaggc aagcgaatcc gtattaaaca
     1981 tgcaatctct ctccttcaga cgacgggttt accggcgtga tgcgcagccc ccgctgcaca
     2041 agccgcggac atgccccaag gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag
     2101 ctttcaccgc ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga
     2161 acaacgagca cgccccagcg tgtgagagga aaaggcggca ccccagtgcg gtcttttccg
     2221 gtgttccggc ccccgtcaga gttcgccata gcgagcggct gaccacccgc aaaatcagga
     2281 gcagctttgc tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg
     2341 tgagcctgac gggggccggg tgaatttctg gcgtggccgg cgtccagccg gacagagcca
     2401 gaaagagggg aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa ggtcttttcc
     2461 cctgaagtca gccacgcccc agcggggatg acttgcattt ctccgcaagg agaaaaaagc
     2521 aacagagata ggttaacgta aaatgttcag cgaaaaaccg acatggttca cctatcctga
     2581 taattgatcg tcagggaata gaaagactta atcaggggac aatatcccac atcagcgcgt
     2641 ccggtgaccg tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg
     2701 ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg
     2761 tggataagca ggggactaac atgtgaggca aaacggcagg gccgcgccgg tggcgttttt
     2821 ccataggctc cgccctcctg ccagagttca cataaacaga cgctttttcg gtgcatctgt
     2881 gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa
     2941 gatccccacc gtttccggta ggtcgctccc tcttgcgctc tcctgttccg accctgccgt
     3001 ttaccggata cctgttccgc cgttctccct tgcgggaagt gtggcgcttt ctcatagctc
     3061 acactactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga
     3121 actccccgtt cagcccgact gctgcgcctt atccggtaac tttcatcttg agtccaaccc
     3181 ggaaagacac gcaaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     3241 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     3301 aggacagatt tggttgctgt gctctgcgaa agccagttac ccggttaagc agttccccaa
     3361 ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag
     3421 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga
     3481 tccccgtcag ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat
     3541 aatttgcctg acatgcggcc agaagaaagc cagcaacaat caatataaaa agaaaaatag
     3601 tctttacctt tttcatatac tttccctgta tttaaagact ccggcattca tccctgaaca
     3661 ccggaagtca gtatttcaaa tcatccttga taattttttg ttaccagtta ccgtcccgat
     3721 aatcaaaagc tatataacaa ttatccacat caggttttat ttgattttta aaaaatggct
     3781 gtagtaaagg aacccattct ttcttcaaat caaactcacc attattaata ttatccttta
     3841 aaggcattcc caagctttca atgacagaac catcgtcacc aaagtcttta gaatactctt
     3901 cccctttaaa ctcttcagtt ttcttatcaa accatgtcaa acgtaattta agccccatat
     3961 ttacctctca aagatatttt ttaatgttcc gttttggatc tggcccttta acctgcttac
     4021 ccgttttggg atcaaatgcg cctaggtgtt cgccatcact ggcacgatac ccttcaagct
     4081 caccgtgctg ggaatcccat tcataaattt tacgtttttt atcgccatac catcgggccc
     4141 gcttaccacc accaccttgt ttaggtgttt taggatcacc tcgctttaac tctccaagtc
     4201 cctttatgtc ttctgtttta ggtactggat gataatcatg gccgtagtct ttagtcccct
     4261 tgcgaggctt gttcttttcc tcatttaact tattttctgc atccttcgct tttttctcct
     4321 tttgtttccg gctctccatc gccccggtaa gtgcagcctt atctctggat aaggattcat
     4381 tcattgcgtt taaagatgcc tgagccgctt ttaatttctc attctgattc tgaatatcct
     4441 tatcaagata gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag
     4501 tcacaaaatc atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag
     4561 cagcccttgc accggggagc gccttctctg tcgatgcaat gcggctgttt aaggtcgcaa
     4621 tatttttatc ttcggcatcc agctcagctt tcgctttatc atagtctctt ttcagcccct
     4681 cttccggatg agcggcgtcc catgcctgct ggcgacgctt ttcttcctcc tgacgctgtt
     4741 ttacctgtgc cggggtaaga acatctgtca ccgacacata aaccggcttc tgtccggtct
     4801 ctttcgggaa acgaataacg gcctcacggg agttaccacc ggctgtaaaa ccagccggac
     4861 gtgaatcacc tttttcagca acaatgcctt ttggcggggc tttcgctgtc ggaacacctt
     4921 tcggtacgct cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac
     4981 gttttgtcgg tttcgcatcc accacaggga cactcattgg ccgacctgcg acaaccgcaa
     5041 tatgctgccg ctcatccttc accacatcca caacccgttg tctgacacga accgtcgcct
     5101 ggtccagtgg taaagtactt gccggagtct ccgtcaccgc atcggccggt aatgacgtca
     5161 taatttttga catcattttc ggatcatctt ttgcaatctc agaaggcagc acaccgtaga
     5221 tcgcaatccc ccacagacca aacttaaacg gccccttcag ggcagccagc acatcagcaa
     5281 cggcggccga caacgcatcg ccggaaactg ataaagccag tccttcagcc cccggagtag
     5341 ccagggcagg cagaccgaag gccatcgcag tggccgaaga ctgtccgcca ccagagctac
     5401 cgctatgatt gccgctgtta ccaccattac caccgggttt actcccgccg ttaccaaact
     5461 cccccggatt atagttatga atatctccat tcggcgtatg cgtcgtaccc cagcccggcc
     5521 cgctgtttgg atccgtacca ccacctgatg aagggccacc tttaccagac gtaccgttca
     5581 cattcccact ggcctgacca ccattatgtc ccagacctga attacccgga cctcgaccgt
     5641 ctccaccact cataccgttt tcctctttga catttaaaac aataaattaa agccaacatg
     5701 tcgcggtagg gatacccgtt accggatacc ccccgcacag atcccggcgt gcgcgattta
     5761 cgcaccgggc tcctgcctcg ggtgtctggc ggtgaaccgc tccacaggcc atggatgaag
     5821 aacccgaacc cttggtagcc atgcggctgc cagtttgttt gctttcgtcc aggtcgtatc
     5881 atccttctgg ctcctgcgcc tgagcgcccg gcgccagagg tttgttacgt gtgtcctgaa
     5941 cttctgcatg gtggggaagt tgcccggtac cgagtgatag ttcaggtatc cctgaaccac
     6001 tctcctgagc cattttccct gttcggggat tgagtaatgc cagcgccttc gcagaccgtc
     6061 tttgatggct ttcagagttg ccgtcatccg atcccggcgg gtctttcgta tcagcatgaa
     6121 cctgccgttg cgatctttcc cgctgatgtg cgtgaacccg aggaagttga acgtttctgg
     6181 tttgcctttt cccctgatgg cacggttttc ggcagcgaag cggccgaact ccatcagacg
     6241 ggttttctcc gggtgaaccg tgagtccgaa ctccctcagt ctgcgctgca tggctatacg
     6301 gaagcgccgg gcatcgtatc gtttgtcgaa cccgatgacg atgtcatcgg cgtatctgac
     6361 cattaccaca ttgcctgtgg catagcgacg tcgccactga tgcgcccaca gatcgaagac
     6421 gtagtggagg tatatgtttg ccagcagcgg tgagatgacc gcaccctgtg gggtgccttc
     6481 ctccgttgct cgccattgac cctcctccga cgtcccggct gtgagccact tacgtatgag
     6541 cctgattacc ctccggtcgc cgatccgatg ctctgtgaac ctgatcagcc attcgtggct
     6601 caccctgtcg aagaactgac tgatgtcggc atccagtacc cagtttacgt tagtgcgtac
     6661 cagccctgtg gccagtgcgt ccagtgcatc gtgctggctt cgcccgggtc tgaacccgta
     6721 tgagaacccc ataaagtcgt tttcatagac tgcgttcagg attttcacca gcgcatactg
     6781 gacgatcttg tcctccagcg aggcgatgcc gagcgggcgt tgttttccat ccgcttttgg
     6841 gatgtagtga cgcctgccgg gctgcgccct gtagctgccc tgatgtagcc tccggtgcag
     6901 atctgttatg ttgttcttca tgtttccggc gtagtccatc cacctgatgc catccactcc
     6961 ggcggccgct ttcctgctca gggagaggaa tgcggcttcc agtgcttcga ctgtcagcag
     7021 gtggaacaat gctgtaaacc gttctttctt ccgctgcttc gcagcttccc gcacgcgtga
     7081 cagcctctgt gacatgcttt cccggctctg tgtccggcgc atgtgtggct gttccgcgtt
     7141 ccccttggcc ccgctccttc gctccactga ctccgctcct ttcgggttgt tcgcctgctt
     7201 cgccgctact atgagcgagt ccgacttctc ctctccgtac atcaccggct atgactcctc
     7261 gtcttcccgg tgcgggccat ctccgacact ggcagatggt cagaggggag atctcccggt
     7321 tcccgcgtag agatcgtatt gacatgccag ggtctcagac cccgccgggt ccatgtggca
     7381 ctcgcagtgt cgcaccctat gatgttgcct tccgttaaca gtacaacgtc ggcacccggt
     7441 aatttaatat acatttcgtg gctcaatggc tggcctgtca acacccctgt caacgcttcg
     7501 ccccatacct cgcggtatgc aacgcatgac tcggggacct tgtggattgc tggtccttca
     7561 atggtcgggg actttcaccc cttgatctct aaccggtctc ccggcgcaca ctgtacatat
     7621 acacagtata attattacct tactcctccc gttttcaggt gtcaatatat gagattagtt
     7681 ctcatcttca ggcacaaaaa agcgcccgga gccacctgta agcagatcgc gtcttttccc
     7741 gaaaatttct catggcaaca aattttatta tttaagacat tcatcacact tacatttcag
     7801 atggtataga cacatctgaa agctaactgg catgaggaga ggacctgcgg ttaaactgtt
     7861 cgggaaacct gagacgcccg gaggccacta tgacgcacct gaaactgact cccgctgaag
     7921 aaaagcgcct ggcggacgcc tttgtcgctc aggcactggc catgggcctg aaagggaaca
     7981 aaacgctgcc aaaggcggta aaacaacagg tgcgggaaag ggtgcttcgt gccattcagg
     8041 acgaacgtga agccaggcgg gcgcaggaaa tgcgtcggga gaaatacgag gctacggaaa
     8101 ccaccttcac ctggcagcca cagcgccgcc ggtaaggggt atttaccata acgtcgcatt
     8161 gcccgcaccg ctcagacggt ctgagggcgt ttccggcggc tacgggataa aacaggcaac
     8221 aaaccacccg gaaaaccgcc ggaaacgcgc ccggtaaatt gtaacccgct gcatgcctat
     8281 gcacgcaact gcatggcggg ccgtttggtg taaaaacgat acgataagcc gtgtttatgt
     8341 aaatgaacgt ggccgtaaag agtgacaaat atacatgtaa cattttttac tgacaggtca
     8401 tcaacccgat gcgtttaata ccctttatcc tgctggcatt gtgcagcccc ttactcctga
     8461 cggcctgtaa gcattccacc acccagccgc cgaaaattta tactctggat aagtcatcta
     8521 aaatcagtag ctacgctgcg gccatcaaga ccgctgttga aatgcagatg tttgaaccag
     8581 aggcctatgc gggaaaaatg tgcaccgtgc ggatatccat acaacgtgat ggcactctga
     8641 acagcgccat tgctgaagga ggagatccga aactctgtaa agcagcaata tcagccgtta
     8701 cccacgccaa aattccgccg gcaccggatg aaaatacctg gcaaatattc aaaaacgccc
     8761 ctctggattt cagaccatga aagcttttac tctgacagtg ctggcggcca tgttcctcac
     8821 tccggtacat gcgataccac taaaacccgt aaccacactg cagccaacct acccggtaca
     8881 ggccgcagca aaacgcatcg aaggtcaggt gaccgccacg tttgatattc tggacgatgg
     8941 acgagtggac aacgtgtcgc ttaccggctg tcaccttttt tttcgggaaa cgctgacggc
     9001 aatgaggcac tggctatatc ccgcagggca cccggcaatg cgacaggtta taaattttga
     9061 attcagtctg aaaaatgacg gcttacagga tccgcgacca gtcaccaccc aaaagctcaa
     9121 tgatgatggt tgccgccagt aacaggtcct ttaacccgca ggctgcaata gtcctggatg
     9181 atgagtgagc ctgtagcagc cctgattagt ggaagcgaag ctgaaaaatg ggcagcaaaa
     9241 gccgtatttc tggcaaatag cgccgccacg gattaaagta gtcatgtgta atacatatga
     9301 ctacaaaggg gcttttatgt catcagcaac atccatcagg ctcgatgaag aacttaagga
     9361 ccggctgaaa acgctggccg atgaccggca ccgctcagcg cacgcgctaa tgctggaggc
     9421 aatcaccgaa tacatcgacc gggaagagaa gcgcagccag tacctgcggg acggacaggc
     9481 cgcatggcag cactatcagg aaacgggtct gcaccttact gcggaagaag cggaagcctg
     9541 gataagtacc tggggaactg agaacgagca ggatgctccg ccatgccaca ggtaacgatt
     9601 tccgcgctgg cacagcgcga tttacaacgc cttcaggact ttctcaaaac caaaaatcgg
     9661 ctggcagcca gaaaaggagg tgaggtgatc gtccgggcta tccagcaact gaaaacattg
     9721 ccagacattg gccgcccggt gccgtttctg ccgctggaat ataaggaact ggtgatcggg
     9781 tttggcgaca gtggctatgt gatgctctac cgccacgacc gggaaatgga ccagattgtg
     9841 atcgtcaccg tcaggcatca gaaagaatcc gggtatccgg gggcagacag cctctaataa
     9901 ccgatgacgg gggcgcaatt cattgcgtgg ccgtcgccgg tttgacaagc gcagcgcgtc
     9961 agaaaaccac atcctgaacg gaaaaacggg taaaattcag cgatacattt accgtgagaa
    10021 attacacacc tttcacaccc gataaaatca ggggtataca gtggcgaaaa cattgctcaa
    10081 cctttcacag gcagcacaag ccgcgggcat taccagaaga acgctataca atcatgtaaa
    10141 acagggaaag gttaccgtgt cacgtgacgg gaaaaacaac cctgtcgtgg atgtgtcaga
    10201 actgatccgt gtatacggaa atgtgaatat ccctgagaaa caaattcccg gcatttcaca
    10261 ccgtgaaaat acacaaaaaa atttcccaca ggaacagctt ctggcaatgc aaaaggaact
    10321 ggccgatctc cggcaggctg tcaccctgat gctggaagat aaaacatccc gggaagaaga
    10381 acgccgccag cacgatgatg agcgccggaa actccaggcc gaagtggaca gattgacaac
    10441 agaactgaca cagaagaaaa aacgcttctg gtccggctgg tttagctgag aacttaccga
    10501 agggaatgga gaacatgacg aagcaggaaa aaactgccct caatatggct aaattcatta
    10561 aggatcagtc gctgctgttg ctggaaaagc tgaacgagct ggacatggat accccggccg
    10621 acatgtgcga acggcttcac gaggatgcgg aaacactctg gcaggtgatg cgggataagc
    10681 tcggggaaga ataacagcac cggccatgcc ggtgctggcg ttattttttg ccgaacggcc
    10741 accaccggcg ggcgggtttg tcctccggca gttcaggcac cggcacgtta aaatagcggc
    10801 tgttatattc ccgcatcagc tccgctttca gcttctcgtt caccgcgctt tccccgtgcc
    10861 gctcgctgac cacctcaatc atcgcgttaa tctgctccgg tgagcacttc ccggcatcaa
    10921 ccacctgcac accgttctcg atggcgaacc gctccacctt tcgatagagg taatcacaca
    10981 gcttcattcc tgtgggtttg tcccgttccc attgcggatt ttcaccggtg aaaaagtacg
    11041 tggctttttc gtcgctgttg agccgctcgt catacatccc gatggatttc atcatcgcca
    11101 gatgctgggc ccggtcgaca aaacagaaaa catacgcccc ggtctgccag gcgagcacca
    11161 ccatgtcttc cagatgctcg ctggttggca taatgcagcg ctcgccgtct ttgtcttcca
    11221 cgtcctcaat aaataccatg tacagcgcca taatccctca ctccgttgtc ataaatttaa
    11281 agcacgcgaa cagcctctcc cggtccagct ccaccgtttt gcgggcctcg ccggtctgtg
    11341 gattcacgat ttcatccacc gccagaatcg cccgctgtac ccggacaagc gtcggccggt
    11401 cagggcagat gtaccagatt tcatcgatgc cgttcgcccg gcggttgaac agatacccgg
    11461 acaccacttc ctgataccgc ctgcggctct tgacggtgcg ctctacctca aatgccaccg
    11521 ttctgccgtc cagctccgcc agcgcgtccg gcactttcat ccctttcagg gccatacggt
    11581 gcaggtaccg ccagccggtg gccccccggc cctccatgga gaggcgcagg gactgcacgg
    11641 ccagccggtg cggcagctgc gccgccgaca cccttgacgg ctgaaacgga ataacgtccg
    11701 tcagcggctc gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc
    11761 tgacctgacg gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccagac
    11821 tgtccaggaa gcggtaaacc ggctgtaccg ccgcaatccc cagccgtcgc gcaaccagct
    11881 ccgccgtgga acacagctct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt
    11941 tccgcgcctg ccgcttcgcg tggctgacgt tg
//
