LOCUS       NZ_CP130163             2572 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Escherichia albertii strain EA04 plasmid pEA04.7, complete sequence.
ACCESSION   NZ_CP130163
VERSION     NZ_CP130163.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36409210
            Assembly: GCF_031236015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wang,S., Guo,W. and Wang,Z.
  TITLE     Whole Genome Sequencing and Characteristics of blaCTX-M-55 and
            blaTEM-141 Producing Escherichia albertii Isolated in Poultry Farms
            in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wang,S., Guo,W. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) Shanghai Veterinary Research Institute,
            CAAS, Shanghai Veterinary Research Institute, CAAS, No.518, Ziyue
            Road, Minhang District, Shanghai 201100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v4; CANU v. v1.7.1
            Assembly Name                     :: EA04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 215.03x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/08/2023 08:50:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 338
            CDSs (without protein)            :: 338
            Pseudo Genes (ambiguous residues) :: 0 of 338
            Pseudo Genes (frameshifted)       :: 91 of 338
            Pseudo Genes (incomplete)         :: 236 of 338
            Pseudo Genes (internal stop)      :: 66 of 338
            Pseudo Genes (multiple problems)  :: 50 of 338
            Pseudo Genes (short protein)      :: 3 of 338
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130163.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="EA04"
                     /isolation_source="spleen"
                     /host="poultry"
                     /db_xref="taxon:208962"
                     /plasmid="pEA04.7"
                     /geo_loc_name="China: Shanghai"
                     /collection_date="2022-05-23"
     gene            1..549
                     /locus_tag="Q0121_RS25685"
                     /old_locus_tag="Q0121_25670"
     CDS             1..549
                     /locus_tag="Q0121_RS25685"
                     /old_locus_tag="Q0121_25670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149494487.1"
                     /translation="MMIVNSCCPQLTTYTPTSCNNVRKPPQILFVEKGYILQENQSATA
                     PKDIPLVTSELFSCRAIAILGENSNWMAHIFEWPSTGQAHTKESMQKMLDTAAEMLGIQ
                     PVELPSCKVFIVCGSDPCDEIDEPLKSALSMYNINAEWFDGSDSTSPIYALAYQDEVGT
                     ANGDITEHHQTKEINSRQI"
                     /name="hypothetical protein"
     gene            complement(819..1143)
                     /gene="mobA"
                     /locus_tag="Q0121_RS25690"
                     /old_locus_tag="Q0121_25675"
                     /pseudo=""
     CDS             complement(819..1143)
                     /gene="mobA"
                     /locus_tag="Q0121_RS25690"
                     /old_locus_tag="Q0121_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /name="mobA"
     gene            1427..1621
                     /locus_tag="Q0121_RS25695"
                     /old_locus_tag="Q0121_25680"
     CDS             1427..1621
                     /locus_tag="Q0121_RS25695"
                     /old_locus_tag="Q0121_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024216200.1"
                     /translation="MNKQQQTVLNMAGFIKGQSLTLLEKLDALDADEQAIMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2109..2270
                     /locus_tag="Q0121_RS25700"
                     /old_locus_tag="Q0121_25685"
     CDS             2109..2270
                     /locus_tag="Q0121_RS25700"
                     /old_locus_tag="Q0121_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007783.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     Typing          complement(1153..1392)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          1651..2181
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.14"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(926..1143)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.151"
                     /coverage="100.0"
                     /evalue="1.42e-48"
ORIGIN
        1 atgatgatag ttaattcctg ctgccctcaa ctcaccacgt atacaccaac aagctgcaac
       61 aatgtaagaa agccacctca aatattattc gttgagaagg ggtatatcct ccaagaaaac
      121 cagtctgcca ctgcccccaa agatatccct ttggttacgt cagagctttt ctcatgcagg
      181 gctattgcca tcttaggaga aaacagtaac tggatggctc atatttttga atggcctagc
      241 acaggtcagg ctcacaccaa agagtccatg caaaaaatgc tcgatactgc agcagaaatg
      301 ctggggattc agcctgtaga attaccttcc tgtaaagtat ttattgtgtg tggctctgac
      361 ccctgtgacg aaatagatga gccattaaaa tctgctttat ccatgtacaa cataaacgca
      421 gaatggtttg atggaagtga ttctacatct ccaatttatg cattagctta tcaagatgag
      481 gttggtactg ccaacggcga tataacagaa caccaccaaa caaaagagat caattccaga
      541 cagatataag tacgtgaaat agtagattca cagctaacag gagtagtcct cagacatcat
      601 ttaccataat cccttaattg tacgcaccac tgaaacgcgc agaacgcgat tctgacgcag
      661 acaccgaaaa aagagatgtt ataccccgga aaaacaggaa ggatgcgtca ggatcgtttt
      721 cggctcgctg catagctatg catgaaagcg aatggtgatc actttgggag cttacggtgt
      781 tcataccgtc tgttttcgac agttttctct ccgggaagct aatctgccat aagcctggat
      841 aacagggcac ggtgatactc cgtaatagcg atcagcacct ccgcatactc cctgtccccg
      901 acacgcttgc cgtcgataaa gagttttttc tgtaacgccc cccagtctca taacttcttt
      961 cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg cccgtaaaaa
     1021 accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt cttcttctgc
     1081 cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac ttcgttttgt
     1141 cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac tagggtaatt
     1201 gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt cagcaagctc
     1261 gaattaccga caaaggcacg gcagtttttt ggcagtgaca gtgttgtctg acagaaatgc
     1321 ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaaca gtcgaaaagt ggtacgcgat
     1381 ataatcacat aacgcagatg acagacagaa cggagaaaac gaaaagatga acaagcagca
     1441 gcaaaccgta ctcaacatgg cgggattcat aaaaggccag agcctgacgc tgctcgaaaa
     1501 actggacgca ctcgatgctg acgagcaggc catcatgtgt gagaagctgc acgaactcgc
     1561 agaagaactc cagaacagca tacagacacg ctttgaagta gaaaatagca cagagagata
     1621 acgctcagga caggatgccg aacggcacaa cagagcgtaa ggagaaaatg tcacataagg
     1681 cgctcttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     1741 gtgtcagctc actcaaaggc ggtaatgcgg ttatccacag aatcagggga taacgccgga
     1801 aagaacatgt gagcaaaaag ccgaaggcag ggacaaaggc cgcgccgatg gcgtttttcc
     1861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1921 acccgacagg actataaaga taccaggcgt ttccccccgg aagctccctc gtgcgctctc
     1981 ctgttccgac cctgccgttt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2041 cgctttctca tagctcacgc tgttggtatc tcggcccggt gtaggtcgtt cgctccaagc
     2101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2161 gtcttgagtc caacccggta agacacgaat tatcgccact ggcagcagcc actgataacc
     2221 gaattagagg agtaagactt gaagtcatgc gcctgttaag gctaaactga aagaacaagt
     2281 tttggtgact gcgctcctcc aagccagtta cctcggctca aagagttggt agctctgaga
     2341 accttcgaaa aaccgccttg cagggcggtt ttttcgttta catagcaaga gattacgacg
     2401 agaaggaaat gatctcaaga agaataaccg gaaccgtcaa gcctttcagg cttaccaacg
     2461 caagtaatta aaacatcatt aaagcaacga tggtgcagct ctgcctgcat attcagccac
     2521 ataccttatg tgtgtaaact gtaaattaga cggtaaacta tattgcaaaa ac
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