LOCUS       NZ_CP130162             4403 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Escherichia albertii strain EA04 plasmid pEA04.6, complete sequence.
ACCESSION   NZ_CP130162
VERSION     NZ_CP130162.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36409210
            Assembly: GCF_031236015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Wang,S., Guo,W. and Wang,Z.
  TITLE     Whole Genome Sequencing and Characteristics of blaCTX-M-55 and
            blaTEM-141 Producing Escherichia albertii Isolated in Poultry Farms
            in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Wang,S., Guo,W. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) Shanghai Veterinary Research Institute,
            CAAS, Shanghai Veterinary Research Institute, CAAS, No.518, Ziyue
            Road, Minhang District, Shanghai 201100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v4; CANU v. v1.7.1
            Assembly Name                     :: EA04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 215.03x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/08/2023 08:50:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 338
            CDSs (without protein)            :: 338
            Pseudo Genes (ambiguous residues) :: 0 of 338
            Pseudo Genes (frameshifted)       :: 91 of 338
            Pseudo Genes (incomplete)         :: 236 of 338
            Pseudo Genes (internal stop)      :: 66 of 338
            Pseudo Genes (multiple problems)  :: 50 of 338
            Pseudo Genes (short protein)      :: 3 of 338
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130162.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="EA04"
                     /isolation_source="spleen"
                     /host="poultry"
                     /db_xref="taxon:208962"
                     /plasmid="pEA04.6"
                     /geo_loc_name="China: Shanghai"
                     /collection_date="2022-05-23"
     gene            52..1125
                     /locus_tag="Q0121_RS25660"
                     /old_locus_tag="Q0121_25650"
     CDS             52..1125
                     /locus_tag="Q0121_RS25660"
                     /old_locus_tag="Q0121_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418401.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_106121029.1"
                     /translation="MFDLTHRKNYEFNQDCILDGVVKKSLVYGKNGIGKSNLGLALFDI
                     VAHLTEFNVNDGMYNNYINAVNNSGIARFKYDFKFGDNIVTYLYGKRSVKELVFEELFI
                     NGRLVTKFNRVLDSNAYFSLSGTETLNTALAESKISIINYIKNNAILNDGIEKDLFNQF
                     IVFVEKMLYFRSLDSNNYLGLEMGRHLITPDIVERGNVDDFERFLNKAGVKCKLCVMKI
                     QSNNGVVEDIGFDFGSKQIPFYGVASTGTKSLALFYYWLQRFKDEKCVSFIFVDEFDAF
                     YHHSLSMLIVKEMKKAGAQVIITTHNTNVMTNELLRPDCYFIMDDKGITPLFEKTDKEL
                     REAHNIEKMYKAGTFND"
                     /name="ATP-binding protein"
     gene            1118..1900
                     /locus_tag="Q0121_RS25665"
                     /old_locus_tag="Q0121_25655"
     CDS             1118..1900
                     /locus_tag="Q0121_RS25665"
                     /old_locus_tag="Q0121_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_309267051.1"
                     /translation="MTERILFVFEGEKTEKTITDSLLKKFIENDKRVVLSSFKTDIYSL
                     YKLMSEDEDLDVFAVLKEKNPDLVDLDRDSFSQLFLFFDYDGHAPAASDEKIDKLLSFF
                     NEETENGKLFISYPMVESIKCISHIDAIEDFCRHTVKICDCSKFKGYVAEYAHKSLIHF
                     NLYSDEIWNDVVRMHCVKSNFIMKGNMIFPSNYFSQKDIFGMQKSKYIDPNGSVSTLSS
                     FPMLLLDFFGHQRLFVLVSGEQIEDGDVLSSEEAQRTI"
                     /name="hypothetical protein"
     gene            complement(2064..2177)
                     /locus_tag="Q0121_RS25670"
     CDS             complement(2064..2177)
                     /locus_tag="Q0121_RS25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001712890.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(2728..2931)
                     /locus_tag="Q0121_RS25675"
                     /old_locus_tag="Q0121_25660"
     CDS             complement(2728..2931)
                     /locus_tag="Q0121_RS25675"
                     /old_locus_tag="Q0121_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041634.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQATMCERLHELA
                     EELQNSIQTHFEAQYGIGVEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3730..4143)
                     /locus_tag="Q0121_RS25680"
                     /old_locus_tag="Q0121_25665"
     CDS             complement(3730..4143)
                     /locus_tag="Q0121_RS25680"
                     /old_locus_tag="Q0121_25665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241651399.1"
                     /translation="MQSLPLNASLNDLADREEPSMRKNFVTCRAIPIYHNVSLRAPLKH
                     AQRDSDAKTAKRGVFPGLNRKNASGTFSARCMTMHETEWRSVWARPLSLRLIFGSFLSV
                     CVTFHARTHPSATTFNSPPLAHTQNTDGHTDVS"
                     /name="hypothetical protein"
     Typing          3054..3132
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.5"
                     /coverage="83.0"
                     /evalue="7.81e-24"
     Typing          complement(2106..2596)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.331"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 atgatataca gatttgaagt tgaggggttt aaggggtttt cagacaaatt agtgtttgat
       61 cttactcata ggaagaatta tgaatttaat caagattgta tacttgatgg tgttgtcaaa
      121 aaatcattgg tatacggtaa aaatggtata gggaaaagca acttagggct tgctcttttt
      181 gatattgtcg ctcacttaac tgagtttaat gttaatgatg gcatgtataa taactatata
      241 aatgcagtta acaactctgg tattgcgaga tttaagtatg attttaaatt tggtgataat
      301 attgtaactt atttatatgg gaaaaggagt gtgaaagaac ttgtttttga agagttgttt
      361 atcaatggca ggcttgtaac taaatttaat agagtgcttg attcaaatgc ctatttctca
      421 ttatcaggaa cagaaacttt aaacactgct cttgcggagt ctaaaatatc gatcattaat
      481 tatataaaaa ataacgccat ccttaatgat ggtatcgaaa aagatctatt taatcagttt
      541 attgtttttg ttgagaaaat gttgtacttc agatctcttg attcaaataa ttatcttgga
      601 ctagagatgg ggcgacacct aatcactccg gatatcgtgg agagaggtaa tgttgatgat
      661 tttgaaaggt ttttgaataa agcaggggtt aaatgtaaac tttgcgtaat gaaaattcaa
      721 agtaataatg gagttgttga agatattggc tttgactttg ggagtaagca aattccattc
      781 tatggagttg cgtccacagg tacaaaatca ttagctcttt tttattactg gttgcaacga
      841 tttaaagatg aaaaatgtgt ttcatttatc tttgtagatg agtttgatgc tttttatcat
      901 cactcgcttt ccatgcttat tgttaaggag atgaaaaaag ccggcgccca ggtgataatt
      961 acaacgcata atacaaatgt catgacgaat gagttgctta ggcctgattg ttacttcatt
     1021 atggatgata aagggattac tccacttttc gagaaaacag acaaagagtt gcgagaagct
     1081 cataatatag agaaaatgta taaagcgggt acatttaatg actgagcgga ttttatttgt
     1141 ttttgagggg gagaaaacgg agaagactat cactgatagt cttttaaaaa aattcattga
     1201 gaatgataag agggttgttc tttcttcatt taagacagat atatattcac tttataagtt
     1261 gatgagtgag gatgaagatc ttgatgtatt tgccgtgttg aaggaaaaaa atcctgactt
     1321 agtagatctt gatagagatt ctttctctca gctttttctg ttctttgatt atgatgggca
     1381 tgctccagct gcttctgacg aaaagataga taagctacta agttttttta atgaggaaac
     1441 tgaaaacggt aaattattta tcagttatcc tatggttgag tctataaaat gtatctcgca
     1501 tattgatgcg attgaggatt tttgtaggca tacagtaaaa atatgtgatt gttctaagtt
     1561 taaggggtat gttgcagaat acgctcataa gtcattgatt cactttaatt tgtattctga
     1621 tgagatttgg aatgatgtgg ttaggatgca ttgtgtgaaa tctaatttca taatgaaggg
     1681 gaatatgatt tttccttcta attatttttc acagaaagat atattcggta tgcagaaatc
     1741 aaaatatata gatccaaatg gctctgtatc tacattaagt tcattcccga tgttactttt
     1801 agactttttt gggcaccaaa gattgtttgt gctggtcagc ggtgaacaaa ttgaggatgg
     1861 agacgtattg agttcggagg aagcccagcg tactatttga aaagaaaaaa ccacccggca
     1921 aggtggtttt ttcgaaggtt cagtaaccgg caagtttctg aaccgaggta actggctaat
     1981 agaatagagg agctcagtca ccaaaatttg ttctttcagt ttagccttaa ccggcgcata
     2041 acatcaagac taactcctct aaatcagtta ccaatggctg ctgccagtgg cgttaagtcg
     2101 tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2161 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2221 caacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2281 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2341 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2401 tgctcgtcag gggggcggag cctatggaaa aacggcatcg tcgcagcctt ttacggtcgt
     2461 gctcttttgc ccacatgttc tgtcctggtt atcccccgat tctgtggata aactgcctgc
     2521 cgttattaga gagccgccac cgctcgccgc agccccagcg accgaacgta gtgagcgagt
     2581 gggcgaggaa gcggaatttc tccgcccgtt tgctttgagc acttacgcgc cagtgtcttt
     2641 ctcaggtcac tgtcctgacg cgccctgcgg gcttgtctgc tggcgaaaac gctcacggac
     2701 aagccgcgac cgctttcgcc agcgtcttta tggttgttcc actccaatgc cgtactgcgc
     2761 ttcaaaatgc gtctgtatgc tgttctgcag ttcttccgcc agttcgtgca gccgttcaca
     2821 catggttgcc tgttcgtcgg catcgagggc atccagcttt tcaagcagtg tcagactctg
     2881 gcttttgatg aatcgcgcca tgttaagtgc ggtttgctgc tgtttgttca ccttctgtcg
     2941 ttctccgttc tgtctctgta ttcagcatca tgtgattatg ccgtgttccg ttctttgcct
     3001 ggacagtgag cgtttttccg gttgtttgat gttcatccgt tcatcacatc gtgggtgtcg
     3061 gggtaaagcc ctgaccaggt tgtaatcgga tgaacgtgcg tgcgcggtta tacgattaca
     3121 catcctgtcc cgttcatggg gtcgtgttac cggcagttgt tgcgtcgctc ctgtttcgtg
     3181 ccgtaaatct tttgcggatg tgacggggta cagttgtacc ccctgccgga ctgttgccac
     3241 cagggcgacc gtatccggct gcctgacaca ggacaggatg tgtaattgta ataccgtcac
     3301 ccgtgacgct attacaatta cctcctgctc aggggttcac cccgataccc cggcgtaatg
     3361 ctctgacggc cagcgcaaat ttattcgctt tttctgagag tcctgtagcc cgaaccagga
     3421 ccggctccgc gcctgaaatt cccctctcct ctgacctgca tttaccacac ctgtcgcaaa
     3481 actgcgtttt gcgtgatttt actttaaatt cagtgcgtta tagtggcgtt tatctggcaa
     3541 aaaattctat tttgtcggat gtggtgggct gatgatgggc taactgtggg cgtagaagcc
     3601 catcatcagc ccacttttgt gggttttata ttgataatca tagtgatagc aaaagtgtgg
     3661 gcgttttgtg ggctaataga gtgggctgat ggtggcctct tatttgtatg aaatataacg
     3721 gataaatttt tatgatacat cggtgtgccc atcggtattt tgtgtgtggg ctaatggtgg
     3781 gctgttgaat gtcgttgctg acggatgtgt tctggcatgg aatgtgacgc agacagaaag
     3841 aaaactgcca aaaataagcc tcagggacag cggacgcgcc cagaccgatc gccattcagt
     3901 ttcatgcata gtcatgcaac gcgctgaaaa cgttcctgac gcatttttcc ggtttagccc
     3961 ggggaaaaca cctctttttg cggtttttgc gtcagaatcg cgctgagcgt gtttcagcgg
     4021 tgcgcgtaat gagacgttat ggtaaatcgg tatggcacgg caagttacaa aatttttacg
     4081 cattgaaggc tcttcgcggt cagccaggtc gtttagcgag gcattgagcg gtaatgactg
     4141 catacgtctt cccactcgcc gtagcgaaag cgacggtagg cacgaacgaa tacgctttta
     4201 cgtttcattt tgtactctcc tgtcttcgtc agttgtagga actgacacct ataaaaaaca
     4261 gcaagcatgc tgagtttcat aggactgcga gagccttcgc ttgcaacttt aattgcagcg
     4321 ataagaaaaa tcaatatcta tatttggtgt aattttttta cggtgccatg atatagttac
     4381 ggcatctttg atgtgaggct ttt
//
