LOCUS       NZ_CP130161             5574 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Escherichia albertii strain EA04 plasmid pEA04.5, complete sequence.
ACCESSION   NZ_CP130161
VERSION     NZ_CP130161.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36409210
            Assembly: GCF_031236015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Wang,S., Guo,W. and Wang,Z.
  TITLE     Whole Genome Sequencing and Characteristics of blaCTX-M-55 and
            blaTEM-141 Producing Escherichia albertii Isolated in Poultry Farms
            in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Wang,S., Guo,W. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2023) Shanghai Veterinary Research Institute,
            CAAS, Shanghai Veterinary Research Institute, CAAS, No.518, Ziyue
            Road, Minhang District, Shanghai 201100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v4; CANU v. v1.7.1
            Assembly Name                     :: EA04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 215.03x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/08/2023 08:50:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,681
            CDSs (with protein)               :: 4,681
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 338
            CDSs (without protein)            :: 338
            Pseudo Genes (ambiguous residues) :: 0 of 338
            Pseudo Genes (frameshifted)       :: 91 of 338
            Pseudo Genes (incomplete)         :: 236 of 338
            Pseudo Genes (internal stop)      :: 66 of 338
            Pseudo Genes (multiple problems)  :: 50 of 338
            Pseudo Genes (short protein)      :: 3 of 338
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130161.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="EA04"
                     /isolation_source="spleen"
                     /host="poultry"
                     /db_xref="taxon:208962"
                     /plasmid="pEA04.5"
                     /geo_loc_name="China: Shanghai"
                     /collection_date="2022-05-23"
     gene            join(5262..5574,1..11)
                     /locus_tag="Q0121_RS25615"
                     /old_locus_tag="Q0121_25605"
     CDS             join(5262..5574,1..11)
                     /locus_tag="Q0121_RS25615"
                     /old_locus_tag="Q0121_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>654
                     /locus_tag="Q0121_RS25620"
                     /old_locus_tag="Q0121_25610"
                     /pseudo=""
     CDS             220..>654
                     /locus_tag="Q0121_RS25620"
                     /old_locus_tag="Q0121_25610"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            707..1249
                     /locus_tag="Q0121_RS25625"
                     /old_locus_tag="Q0121_25615"
     CDS             707..1249
                     /locus_tag="Q0121_RS25625"
                     /old_locus_tag="Q0121_25615"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_275363937.1"
                     /translation="MGADFEKKLKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDA
                     ISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGG
                     IFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKG
                     ENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1256..1489
                     /locus_tag="Q0121_RS25630"
                     /old_locus_tag="Q0121_25620"
     CDS             1256..1489
                     /locus_tag="Q0121_RS25630"
                     /old_locus_tag="Q0121_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1773..1901)
                     /locus_tag="Q0121_RS25635"
                     /old_locus_tag="Q0121_25625"
     CDS             complement(1773..1901)
                     /locus_tag="Q0121_RS25635"
                     /old_locus_tag="Q0121_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_309267050.1"
                     /translation="MRKCIYHNVALRVPLKRVQRDHGSRQVKTATNHPKNCRDRAW"
                     /name="hypothetical protein"
     gene            complement(1898..2491)
                     /locus_tag="Q0121_RS25640"
                     /old_locus_tag="Q0121_25630"
     CDS             complement(1898..2491)
                     /locus_tag="Q0121_RS25640"
                     /old_locus_tag="Q0121_25630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012537770.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="hypothetical protein"
     gene            complement(3085..3999)
                     /locus_tag="Q0121_RS25645"
                     /old_locus_tag="Q0121_25635"
     CDS             complement(3085..3999)
                     /locus_tag="Q0121_RS25645"
                     /old_locus_tag="Q0121_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_042007279.1"
                     /translation="MPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYIWSQKA
                     SSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHGDFIHNINKAKKRYE
                     NEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEASRALWPRLTYRV
                     LKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIREKINK
                     IEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTILDQLYA
                     LTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     gene            complement(4225..4338)
                     /locus_tag="Q0121_RS25650"
                     /old_locus_tag="Q0121_25640"
     CDS             complement(4225..4338)
                     /locus_tag="Q0121_RS25650"
                     /old_locus_tag="Q0121_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531573.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(5008..5199)
                     /locus_tag="Q0121_RS25655"
                     /old_locus_tag="Q0121_25645"
     CDS             complement(5008..5199)
                     /locus_tag="Q0121_RS25655"
                     /old_locus_tag="Q0121_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          4836..4920
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(4267..4825)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.474"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1587
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5230..5573
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.609"
                     /coverage="97.0"
                     /evalue="2.5299999999999998e-55"
ORIGIN
        1 atgatagtta aatttcatgc cagggggaaa ggcggtggaa gtggcccggt cgattacctg
       61 ctggggcggg agcgtaaccg cgaaggcgca acggtgctcc ggggtaaccc ggaagaaatc
      121 cgggaactca ttgatgccac gccattttcg aagaaataca cgtcaggcgt tctgtcgttc
      181 gcggagaagg agttaccgcc gggtgaacgc gaaagagtga tgacgagctt tgagcgtgtt
      241 cttatgcccg gtctcgaaaa agaccagtac agcattctct gggtggaaca ccaggataag
      301 gggcggctcg aactgaattt tgtcattccg aacatggaac tacagagcgg taagcgcctc
      361 cagccgtact acgaccgtgc ggacaggccg aggataaatg cctggcagac gctggttaat
      421 catcattacg ggctgcgtga cccgaacgca ccggagaaca gacggacgct tgtcacaccg
      481 aataatctgc caaaagcgaa acaggaggcc gcagaagcga ttacacgggg tcttgaggcg
      541 ctttaccgtg ccggagcaat aaaaacgcgt caggatgtca ctgaggcgct cacagcggca
      601 ggttttgagg tggtgaggac aaccagaagc agtatcagta ttgccgaccc ggaggggggg
      661 cggaatctca ggctaaaggg ggcgctgtat gagcaatctt ttacaaatgg gagcggactt
      721 cgagaagaaa ctgaaagagc gagccgcttc taccgagaac atgctgaaca gcgagttcag
      781 gaagctagaa gagtctgtag acaaggcact gagcttaaac aggcagaaaa tcagagacgc
      841 tatcagcgaa catacaacat ccgtgaaaca gcaactggat acgctgagca ccaccgtgag
      901 cacgcagctc agcaccacag aggccgaact gtcccggcag cagaaaaacc tgctctggca
      961 ggtgatcaaa gggcgggtac tgtttccggc gctgaccgcg ctcagcgtca cgggcggcat
     1021 tttcctgggg tgctgggggc tgattcagtg gcaggaaagc aggattgcga aaaatatctt
     1081 gaccatcaga gagcaggaaa acacgctggc gaaactcgag gcaaaaacct ggggggtgac
     1141 gttcgtcaat ggcgagaacg ggaaatttct ggtgctgccg gacggggtga aaggggaaaa
     1201 cacctggacg gtgggcgaca agaacgccgt gaggctggtc agggagtaaa cgagtatgac
     1261 ggaactggaa gggcatttgc tgaacgcctt agagcatttg caacaggact atatgcggcg
     1321 gctgaacgag tgggagagcg ccttcgcaga attgcagaag atgcacgcgg ttacgcaaca
     1381 gaacaacgcg atactgaacg agcgtgtcgt gaacttgagt cagcaggtgc agctattagc
     1441 cggacaagtc gaccgcttga gtcggttatt tatcacgaac aacagataag ggatgaacag
     1501 gagcgtcagg cgcaggagct tgcccggcgc tggcatgaag agcaggaacc cgaagcgcgg
     1561 atctggcgtg gcccgtcaat ggggatgtga ccggggtacc ccctgaaatc gagccagttt
     1621 gtggcacgcc ctacccggat ggcgccaatg ttaatgtacg tacattagca ttaacaatat
     1681 ccaaacctcc ccattttgcc ccgttcgacg gctggaacgt gcgaaactga tcgtcattca
     1741 gtttcgcgca taactatgca tgaggttaaa atttaccagg cgcgatcgcg gcagtttttc
     1801 gggtggtttg ttgctgtttt tacctgtctg ctgccgtgat cgcgctgaac gcgtttaagc
     1861 ggtacgcgca atgcgacgtt atggtaaatg catttcctca taggccgatt atttcataga
     1921 tgtttgactc tctatcttct tttggttgca gatgtatcag gcgactacag tacagtacat
     1981 ctaagccgaa cctaatctgg tttggctgga cacctgtata gcttctcagt gtttcatgtg
     2041 tcaccttaac agtattattt gactgataac gaaggcttat aattgttaaa tatagtttta
     2101 atgccacaaa tggggccatc cccctgttcg atatggtgtt tagttttttc ctgatagtat
     2161 ctactggtac ttttgcccac ctatcatcat ttggctgtac tgttaattca taggagttaa
     2221 caaacatctc tctatctatc tttataagac catctttctc aagtttggct actcctttca
     2281 ctatcatggg gcggcttagt ccagtcagtc tttccattcc ggtaaatgat actgaggctg
     2341 ttcttgaatg gaaatcaatc aagacggata gagctaacaa gcatttcatt gatgcaatgc
     2401 cgccccctat ctcatttgaa cgaacattgt ttacaagcca tgagcttcgg gcccaccagt
     2461 ttggaagcct gccaaatttt ctaccgctca tttttacccc attttcggtc aaggagagat
     2521 ataaatagct ttattatcat atacatgaaa accattgcac ctaaagatac aacagaaacc
     2581 aaaacctccg gctttgcgga agttagcgtt accagcaaat tgttgagggt ttccaattgg
     2641 tcctccagtc gcatactaag ttgttgtgtt ttaactttat atacgcttat tagaataaat
     2701 ctagataaag caatatttgg tgggtgcttg ttttcccata taaaacatgg tgttatcttg
     2761 tttttctcgt tttttaactg gtaaatgtaa atttgataat gctggtttaa tcggcgtttt
     2821 tagttatttt tgttgtgtgt tttttgatct attggtaaat ggtggatggt aagctttggc
     2881 gtgatcatgg gaaatagttt gctctttttc ctgatgatga tgtatcatgt atggcagtga
     2941 agccttgagc tttcgcttta atgcattgtg aaatttttat agattcacaa tgacattaag
     3001 gccacgctcc accgaaaggt gataatggca agagatttgg atgaggctcg acaggaaact
     3061 gtcgggcctt ttctattttg tgatctaaaa cttgtgaaat gcgatattgt tggtatttcg
     3121 ttttacaaca cccgttagct ttccgtcagc agttagtgcg tatagttgat ctagaattgt
     3181 gttcatctca tctgttgtgc tattgattat tcctttgcct agatgcaatt cggcctcttt
     3241 ttgagaacat attcttttgg cgtgataaaa ggaaccccac ttcgttaatg atagatgtac
     3301 ctgtgggttt atcgttttta tcagtccttc tattttgttt attttttctc ttattgtttc
     3361 tactgcagat tgcaaatttt tagagtgtat ttttatccat atgcaatcat tatgcgataa
     3421 cctatttctg tagtttctga tctcattcag ctcgtttaag aaatcaattc tggctgtgcc
     3481 ttcatctttg cttctatcta atcccttcag tactctgtag gtgagcctgg gccataaggc
     3541 tcttgatgct tcagatcttg attgtgtatg aaaagcatcg ctcaaaaccg cttgccatgt
     3601 gtaaaaatct gtgtgtgcaa tgatgtcatc atgaggtgca attattttgg tatttgccat
     3661 tgatgggttt tttttcttga attcattctc atatcttttt tttgctttgt ttatattatg
     3721 tatgaagtca ccgtgtttgc tttttgatgt gtcggtgtat acattatccc accatttttg
     3781 gcctattagt tttgtcgctt ctttatcaat cgagtttctc aaagtaactt cgagttgttg
     3841 cagtaatgga tatatggcag atgaggcttt ctggctccat atatatatcc cgtagcactc
     3901 cctatcagac aggttattac caaacgtgct ttttatagca tttatttttc cttgggatat
     3961 aaatggtatt acatgagggt aatgaattgt atttggcata tccatctcta tcatacactt
     4021 agtggtgatt ggatgttgcc atacacgtat ggatagttgt agagtgttaa acgaaaaaac
     4081 cgccttggcg ggcggttttt cgaaggttca aagagttggc gctctgtgaa ccaaggtaac
     4141 tggcttggag gagcgcagtc accaaaatct gttctttcag tttagcctta acaggtgcat
     4201 aacttcaaga caaactcctc taaatcagtt accagtggct gctgccagtg gcgttaaggc
     4261 gtgccttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4321 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4381 ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4441 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4501 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcatc tgtttttgtg
     4561 atgctcgtca ggggggcgga gcctatggaa aaacgccagc ggcgcggcct tcttcctgtt
     4621 cctggccttt tgctcacatg tttcttttgg cgttatcccc tgattctgtg gataaccgca
     4681 tcaccgtctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgtagcgagt
     4741 cagtgagcga ggaagcggaa gagcgcctga tgcggcattt tctccttacg catctgtgcg
     4801 gtatttcaca ccgcatatgg tgcacttttc ttataatccg cactgatgcc acatagttaa
     4861 gccagtatac actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacacccgct
     4921 aaaacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctc acaggcaagc
     4981 tgtgaacgtc tccgggagct gcatgtttca gttgctttca ccgtcatcgc cgaagcgtgc
     5041 gaggcaacta cggtagagtt cgtcggcgtg gtcgtgaagt gattcgcaga tgtctgcctg
     5101 ttcgtccgcg tccagctcgt tgagtttttc cagcagggtc agcgtctggc ttctgataaa
     5161 gcgggccatg ttaagggctg ttttttcctg tttggtcatg gtcttaaggc tccttaatag
     5221 gggatttttt agggggaatg ataccgatga aacgcgagaa gatgctcacg atacgggtta
     5281 gcgatgagga gcacgcccgg ttactggaac gctgcgaggg taaacgactg gcagaatgga
     5341 tgcggcgggt ctgtctgggg gagccggttg cgcgcacagg aaaactgccg acactatcgc
     5401 cgccattgct gcggcatctg gctgccatcg ggaacaatct gaaccagaca gcccggaagg
     5461 tgaacagcgg ccactggtct tcgattgacc gggttcatgt ggtggccgct ctgatggcca
     5521 tcgagggtga attgcgccag ttgcggcagg ctgtcaggga acagggggga cggg
//
