LOCUS       NZ_CP130078             8251 bp    DNA     linear   CON 30-JUL-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Schwarzengrund strain
            SEWLSH-25 plasmid unnamed1.
ACCESSION   NZ_CP130078
VERSION     NZ_CP130078.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35361205
            Assembly: GCF_030505755.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Schwarzengrund
  ORGANISM  Salmonella enterica subsp. enterica serovar Schwarzengrund
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Sopovski,D., Commichaux,S., Zhao,S., Grim,C.J., Foley,S.L. and
            Khajanchi,B.K.
  TITLE     Complete genome sequences of 17 Salmonella enterica serovar
            Schwarzengrund strains carrying IncFIB-IncFIC (IncFII) fusion
            plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Sopovski,D., Commichaux,S., Zhao,S., Grim,C.J., Foley,S.L. and
            Khajanchi,B.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2023) Office of Applied Research and Safety
            Assessment, CFSAN, U.S. Food and Drug Administration, 8301 Muirkirk
            Rd MOD1, Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hybrid assembly using Unicyler v. not specified
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 455.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/30/2023 12:12:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,982
            CDSs (total)                      :: 4,860
            Genes (coding)                    :: 4,685
            CDSs (with protein)               :: 4,685
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 93 of 175
            Pseudo Genes (incomplete)         :: 108 of 175
            Pseudo Genes (internal stop)      :: 32 of 175
            Pseudo Genes (multiple problems)  :: 51 of 175
            Pseudo Genes (short protein)      :: 1 of 175
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP130078.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Salmonella enterica subsp. enterica serovar
                     Schwarzengrund"
                     /mol_type="genomic DNA"
                     /strain="SEWLSH-25"
                     /serovar="Schwarzengrund"
                     /isolation_source="stool"
                     /sub_species="enterica"
                     /db_xref="taxon:340190"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA"
                     /lat_lon="37.0902 N 95.7129 W"
                     /collection_date="2013"
                     /collected_by="Wisconsin State Laboratory of Hygiene"
     gene            <1..793
                     /gene="rfbV"
                     /locus_tag="QPJ64_RS24800"
                     /old_locus_tag="QPJ64_24800"
     CDS             <1..793
                     /gene="rfbV"
                     /locus_tag="QPJ64_RS24800"
                     /old_locus_tag="QPJ64_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abequosyltransferase RfbV"
                     /protein_id="WP_302102032.1"
                     /translation="GPDRNFLASVSLANGDYCWIFGSDDALAKDSLAILQTYLDSQADI
                     YLCDRKETGCDLAEIRNPHRSWLRTDDELYVFNNNLDREIYLSRCLSIGGVFSYLSSLI
                     VKKERWDAIDFDASYIGTSYPHVFIMMSVFNTPGCLLHYISKPLVICRGDNDSFEKKGK
                     ARRILIDFIAYLKLANDFYSKNISLKRAFENVLLKERPWLYTTLAMACYGNSDEKRDLS
                     EFYAKLGCNKNMINTVLRFGKLAYAVKNITVLKNFTKRIIK"
                     /name="rfbV"
     gene            884..1982
                     /locus_tag="QPJ64_RS24805"
                     /old_locus_tag="QPJ64_24805"
                     /pseudo=""
     CDS             884..1982
                     /locus_tag="QPJ64_RS24805"
                     /old_locus_tag="QPJ64_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            2223..3380
                     /locus_tag="QPJ64_RS24810"
                     /old_locus_tag="QPJ64_24810"
     CDS             2223..3380
                     /locus_tag="QPJ64_RS24810"
                     /old_locus_tag="QPJ64_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530319.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="WP_000478553.1"
                     /translation="MFLIFNIVYMLPVAYVHFFGFSLGGVEEIFNGDPSMLADMMVFYV
                     AVLFFYLAGSALLGFGIKNKNIQWIIAFDLPVIVKILFSLVLFSIVLSKVLLYPEGVYS
                     SYAFDSGAMASRVWNISMGLSELGIIIFTFCLVTKNIKFAVVTFLIVSLNLLHGTRIFT
                     LILLLMIIFYIIFINKRISKLKLFIILSIFFSAIVISFLIIFIIRSNVSIDNINIDLIL
                     SPIVYESLFNQISFMRMLDWLHQGAVPFAPQMLFSDSAIFTLPTFLFDEKSSLMYINKF
                     GELSPLGGLSGYASAVIYFSYYYFLWYFILGITSSLLLRFTSSVNFVILSRVIYIYFVC
                     DSLFRFNRDPWFIAIKMFVNNIVFILFVLMIIALKMRMEKRRAIQ"
                     /name="O-antigen polymerase"
     gene            3377..4436
                     /locus_tag="QPJ64_RS24815"
                     /old_locus_tag="QPJ64_24815"
                     /pseudo=""
     CDS             3377..4436
                     /locus_tag="QPJ64_RS24815"
                     /old_locus_tag="QPJ64_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461031.1"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /name="glycosyltransferase family 1 protein"
     gene            4437..5381
                     /gene="rfbN"
                     /locus_tag="QPJ64_RS24820"
                     /old_locus_tag="QPJ64_24820"
     CDS             4437..5381
                     /gene="rfbN"
                     /locus_tag="QPJ64_RS24820"
                     /old_locus_tag="QPJ64_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O antigen biosynthesis rhamnosyltransferase RfbN"
                     /protein_id="WP_000705150.1"
                     /translation="MKITLIIPTYNAGSLWPNVLDAIKQQTIYPDKLIVIDSGSKDETV
                     PLASDLKNISIFNIDSKDFNHGGTRNLAVAKTLDADVIIFLTQDAILADSDAIKNLVYY
                     FSDPLIAAVCGRQLPHKDANPLAVHARKFNYSSKSIVKSKADIEKLGIKTVFMSNSFAA
                     YRRSVFEELSGFPEHTILAEDMFMAAKMIQAGYKVAYCAEAVVRHSHNYTPREEFQRYF
                     DTGVFHACSPWIQRDFGGAGGEGFRFVKSEIQFLLKNAPFWIPRALLTTFAKFLGYKLG
                     KHWQSLPLSTCRYFSMYKSYWNNIQYSSSKEIK"
                     /name="rfbN"
     gene            5382..6821
                     /locus_tag="QPJ64_RS24825"
                     /old_locus_tag="QPJ64_24825"
     CDS             5382..6821
                     /locus_tag="QPJ64_RS24825"
                     /old_locus_tag="QPJ64_24825"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461029.1"
                     /GO_function="GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="WP_001528486.1"
                     /translation="MSVLPVIMAGGTGSRLWPLSREYHPKQFLSVEGKLSMLQNTIKRL
                     ASLSTEEPVVICNDRHRFLVAEQLREIDKLANNIILEPVGRNTAPAIALAAFCALQNAD
                     NVDPLLLVLAADHVIQDDIAFTKAVRRAEEYAANGKLVTFGIVPTHAETGYGYIRRGEL
                     IGNDAYAVAEFVEKPDIDTAGDYFKSGKYYWNSGMFLFRASSYLNELKYLSPEIYKACE
                     KAVGHINPDLDFIRIDKEEFMSCPSDSIDYAVMEHTQHAVVIPMSAGWSDVGSWSSLWD
                     ISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISAIGVSNLVIVQTTDALLVANKDTVQD
                     VKKIVDYLKRNDRNEYKQHQEVFRPWGKYNVIDSGKNYLVRCITVKPGEKFVAQMHHHR
                     AEHWIVLSGTARVTKGEQIYMVSENESTFIPPDTIHALENPGMIPLKLIEIQSGTYLGE
                     DDIIRLEQRSGFSKEWTNERS"
                     /name="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
     gene            6808..8241
                     /gene="rfbK"
                     /locus_tag="QPJ64_RS24830"
                     /old_locus_tag="QPJ64_24830"
     CDS             6808..8241
                     /gene="rfbK"
                     /locus_tag="QPJ64_RS24830"
                     /old_locus_tag="QPJ64_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen biosynthesis phosphomannomutase RfbK"
                     /protein_id="WP_001103637.1"
                     /translation="MNVVNNSRDVIHSSGIVFGTSGARGLVKDFTPQVCAAFTVSFVAV
                     MQEHFSFDTVALAIDNRPSSYGMAQACAAALADKGVNCIFYGVVPTPALAFQSMSDNMP
                     AIMVTGSHIPFERNGLKFYRPDGEITKHDEAAILSVEDTCSHLELKELIVSEMAAVNYI
                     SRYTSLFSTPFLKNKRIGIYEHSSAGRDLYKPLFIALGAEVISLGRSDNFVPIDTEAVS
                     KEDREKARSWAKEFDLDAIFSTDGDGDRPLIADEAGEWLRGDVLGLLCSLALDAEAAAI
                     PVSCNSIISSGRFFKHVKLTKIGSPYVIEAFNELSRSYSRIVGFEANGGFLLGSDICIN
                     EQNLHALPTRDAVLPAIMLLYKSRNTSISALVNELPTRYTHSDRLQGITTDKSQSLISM
                     GRENLSNLLSYIGLENEGAISTDMTDGMRITLRDGCIVHLRASGNAPELRCYAEANLLN
                     RAQELVNTTLANIKKRCLP"
                     /name="rfbK"
ORIGIN
        1 tgggccggat aggaattttc ttgcttcagt atcccttgcg aatggggatt attgttggat
       61 atttggcagt gatgatgctc ttgcgaaaga ctcgttagcg atattacaaa cttatctcga
      121 ttctcaagca gatatatatt tatgtgacag aaaagagacc gggtgtgatt tagctgagat
      181 tagaaatcct catcgttcct ggctcagaac agatgatgag ctttatgtgt ttaataataa
      241 tttagatagg gaaatctatc tcagtagatg cttatctatt ggtggcgtat ttagctatct
      301 aagttcttta atagtaaaaa aagaacgatg ggatgccatt gattttgatg cgtcctatat
      361 tggcacttcc tatcctcatg tatttatcat gatgagcgta tttaatacgc ctgggtgcct
      421 tttgcattat atatcaaaac cactcgtaat atgtcgagga gataatgata gtttcgagaa
      481 gaaaggaaag gccagacgaa tattaattga ttttattgca tatttaaaat tagctaatga
      541 tttttacagt aaaaatatat ctttaaaacg agcatttgaa aatgttttgc taaaagagag
      601 accatggtta tatacaactt tggctatggc atgttatggc aatagtgatg aaaaaagaga
      661 tttatctgaa ttttatgcaa agctaggttg taataaaaat atgatcaaca ctgtacttcg
      721 atttgggaaa ctagcatatg cagtgaaaaa tattaccgtg cttaagaatt ttactaaacg
      781 gataattaag tagccgtaag ctattgacct tccccctgta aacagtgcca gtctaaactg
      841 aagtttccgg cctttcttcc atctcacaga gaggcgcatt gccatgaaaa agacccgtta
      901 taccgaagaa cagattgcat ttgccctgaa acaggctgaa accggcactc gcgtcgggga
      961 agtctgcaga aagatgggaa tttctgaggc cacattttac aactagaaga aaaaatttgc
     1021 cgggctgggc gtgacggaac tgcggcgcct gcggcaactg gaggatgaga atcagcggct
     1081 gaagaagctg gccgctgagc tgagtctgga caaggagatg ctgcaggaga tactgaagca
     1141 aaagttctga ggccggctca gaagcgccag gcggtgacat ttttgcggga ggcttaccgt
     1201 atcagcgtcc ggcgacgatg cgggttgctg atgcagagca gaaccgttta ccactggcag
     1261 agccggcgtg acgatcgggc gataactctg cgtatccggg aaatagcgga aacccggata
     1321 cgctacggtt gcccgcgtat tcatattcag ttgcgccggg agggatggcc tgttaaccac
     1381 aagaaaccca ccggatttat tgtccggaag gcctgaacct gcgcagaaaa cgcccccgca
     1441 gacatgtcag tgcagcacgc cgtcagcagc gcccggtcct gacgcatgtc gatcagtgct
     1501 ggagtatgga ttttgtgtca gataatctgt ttaacgggcg gcgttttcgg gcgctgactg
     1561 tagtggataa ttttagtcgg gaatgcctgg cgatccatgc cggaaaatca taaaaaggcg
     1621 aggatgtggt cagaataatg gaggcactgc gggtgctgga taagcggctg ccggtacgaa
     1681 tccagacgga taacggcagc gagcttatct caaaaagtct ggataaatgg gcgtatgaac
     1741 acggcgtcac aatggacttc tcgcaccccg gaaagccgac agataacccg tttattgaat
     1801 catttaacgg cagtctgcgg gatgaatgac tgaacattca ctggttcctg tcactggaag
     1861 atgcgcagga aaaactcgac aactggcgca gggaatacaa tcatgagaga acgcattcat
     1921 cattaaatga catgactccg gctgaattta tccggagtct ccggaaagac gaagatctct
     1981 gatttagcac ttactgaatt tgggccaggg tcaaattcaa ccgggatctc aaaaaaagca
     2041 tagaactaaa acgggtctat tcatataatg atttcaaaaa atgttgtata ttgagattta
     2101 atgtggactt aacctttcca ttaagaatct gaaatttata gatagagtgt tagctttatt
     2161 tttaattaac aaaaatttaa ttatataata tttgtgagtg tactatgtta ccgcatacat
     2221 ttatgttcct gatttttaat atagtatata tgttaccagt agcctatgta catttttttg
     2281 ggttctccct tggaggggtt gaggagattt tcaacggtga tcccagtatg cttgcagata
     2341 tgatggtctt ttacgtcgct gtcttgttct tttaccttgc gggttcggca ttgttagggt
     2401 ttggtataaa aaataaaaac atacaatgga ttatagcatt tgatcttcca gttattgtaa
     2461 agattctatt cagtttagtt ttattttcta tagtactatc aaaagtattg ctttatcctg
     2521 aaggagttta ctcgtcttat gcctttgatt ctggcgctat ggcgagtcgt gtctggaata
     2581 tctctatggg attatcagaa ctaggtataa taatttttac attttgttta gtaacaaaga
     2641 atataaagtt tgcagttgtt acctttctta tagtctcatt aaatctacta catgggacta
     2701 gaatatttac attaatatta cttctaatga ttatatttta tataattttt attaataaaa
     2761 gaatatcaaa actgaaatta tttatcattt tgagtatttt ttttagtgca attgtgatat
     2821 catttttaat aatatttatt atccgttcta atgtctcaat tgataatata aatatagacc
     2881 taattctaag cccaatagta tatgaatctt tatttaatca aatatctttt atgcggatgc
     2941 tggattggct acatcaaggg gcagttccat ttgcaccaca aatgcttttt tctgattcag
     3001 ctatatttac gttaccgact tttctttttg atgaaaaatc atctttaatg tatataaata
     3061 aatttggaga acttagccct ttaggtggcc tttccggtta tgctagtgca gtaatatatt
     3121 tttcgtatta ttattttctg tggtatttta ttctcggtat tacatcaagc ctattattaa
     3181 gatttacatc gagtgtaaac tttgtaatat tatcgagagt catttatatt tattttgtat
     3241 gtgattcttt atttagattt aatcgtgatc cttggtttat tgccattaag atgtttgtga
     3301 ataatatagt gtttatattg tttgttctta tgattattgc tttaaagatg cgtatggaaa
     3361 aacggagagc tattcaatga tcgttaacct atcacgttta ggtaaaagtg gtacgggaat
     3421 gtggcaatac tcgattaaat ttttaacggc actgcgagaa atagctgatg ttgacgcaat
     3481 aatctgtagc aaggtacacg ctgattattt tgaaaagctc ggttatgcag tagttactgt
     3541 tccgaatatt gttagcaaca catcaaaaac atcgcgactt agaccattag tatggtatgc
     3601 atatagttac tggcttgcgc tgagggtttt aattaagttt ggtaataaaa aattggtgtg
     3661 tactacgcat cacactattc ccttactgag aaaccaaacg ataaccgtac atgatataag
     3721 acctttttat tatccagata gttttattca gaaagtgtat tttcgctttt tattaaaaat
     3781 gtccgttaag cgatgtaagc atgttttaac ggtatcttat accgttaaag atagcattgc
     3841 taaaacttat aatgtagata gtgagaaaat atcagtaatt tataacagcg ttaataaatc
     3901 tgattttata caaaaaagaa aaagagaatt actttttagc tgttggagca agttgggcac
     3961 ataaaaatat tcattcattc ataaaaaata aaaaagtttg gtctgactct tataatttaa
     4021 ttattgtatg tggtcgtact gactatgcaa tgtctctcca acaaatggtc gttgatctgg
     4081 aactaacaga taaagtgatt tttttacatg aagtctcatt taatgaatta aaagttctat
     4141 attctaaagc ctatgcgctt gtttatccat ctattgatga gggttttggc atacctccta
     4201 ttgaagcgat ggcatcaaat actccagtta tagtgtccga tataccagta tttcatgaag
     4261 tgttaaccaa tggtgcatta tatgtgaatc cggatgatga aaaaagctgg cagagtgcaa
     4321 ttgaaaatat agagcagttg cctgatgcaa tttcccgatt taacaactat gtcgcacggt
     4381 atgactttga taatatgaag cagatggttg gcaattggtt ggcggaatca aaataaatga
     4441 agataacatt aattattccc acatataatg cagggtcgct ttggcctaat gttctggatg
     4501 cgattaagca gcaaactata tatccggata aattgattgt tatagactca ggttctaaag
     4561 atgaaacggt tccgttagcc tcagacctga aaaatatatc aatatttaat attgactcta
     4621 aagattttaa tcatggagga accaggaatt tagcagttgc aaaaactctg gacgctgatg
     4681 ttataatttt tctaacgcaa gatgcaattc ttgcggattc ggatgcaatt aaaaatttag
     4741 tttattattt ttcagatcca ttgatagcag cggtttgtgg tagacaactt cctcataaag
     4801 atgctaatcc tcttgcagtg catgccagaa aatttaatta tagttcaaaa tctattgtta
     4861 aaagtaaggc agatatagaa aaattgggta ttaaaactgt atttatgtcc aattcttttg
     4921 ctgcctatcg ccgttccgtt tttgaagagt taagtgggtt tcctgaacat acaattcttg
     4981 ccgaggatat gtttatggcg gctaagatga ttcaggcggg ttataaggtc gcctactgcg
     5041 ctgaagcggt ggtaagacac tcccataatt ataccccgcg agaagagttt caacgatatt
     5101 ttgatactgg tgtatttcat gcttgttctc cgtggattca gcgtgacttt ggcggagccg
     5161 gtggtgaggg tttccgcttc gtaaaatcag agattcaatt cctgcttaaa aatgcaccgt
     5221 tctggattcc aagagcgtta ttaacaactt ttgctaaatt cctgggttac aaattaggca
     5281 agcattggca atctttaccg ttgtctacat gtcgctattt tagcatgtac aagagttatt
     5341 ggaataatat ccagtattct tcgtcaaaag agataaaata aatgtctgtt cttcccgtaa
     5401 ttatggctgg cggcacaggt agccgtttat ggccgctttc acgcgaatat catccgaagc
     5461 agtttctaag cgttgaaggt aaactatcaa tgctgcaaaa tactataaag cgattagctt
     5521 cactttctac agaagaaccc gttgtcattt gcaatgacag acaccgtttc ttagtcgctg
     5581 aacaactccg tgaaattgac aagttagcaa ataatattat tctcgagccg gtaggccgta
     5641 atactgcacc agcgatcgct cttgccgcgt tttgtgcgct ccagaatgct gataatgttg
     5701 atcctctttt gttggttctt gctgcagatc atgtgatcca ggatgatata gcttttacga
     5761 aagctgtcag acgtgctgaa gaatacgccg caaatggtaa gcttgtaact tttggtattg
     5821 ttccaacgca tgctgaaaca ggttatggat atattcgtcg gggtgagttg ataggaaatg
     5881 acgcttatgc agtggctgaa tttgtggaga aaccggatat cgataccgcc ggtgactatt
     5941 tcaaatcagg gaaatattac tggaatagcg gtatgttttt atttcgtgca agctcttatt
     6001 taaacgaatt aaagtattta tcacctgaaa tttataaagc ttgtgaaaag gcggtaggtc
     6061 atataaatcc cgatcttgat tttattcgta ttgataaaga agagtttatg tcatgcccga
     6121 gtgattctat cgattatgca gttatggagc acacacagca tgccgtggtg ataccaatga
     6181 gcgctggctg gtcggatgtg ggatcctggt cctcactttg ggatatatcg aataaagatc
     6241 atcagagaaa tgttttaaaa ggagatatct tcgcacatgc ttgtaatgat aattacattt
     6301 attccgaaga tatgtttata agtgcgattg gtgtaagcaa tcttgtcatt gttcaaacaa
     6361 cagacgcttt actggtggct aataaagata cagtacaaga tgttaaaaaa attgtcgatt
     6421 atttaaaacg gaatgatagg aacgaatata aacaacatca agaagttttc cgcccctggg
     6481 gaaaatataa tgtgattgat agcggcaaaa attacctcgt tagatgtatc actgttaagc
     6541 cgggtgagaa atttgtggcg cagatgcatc accaccgggc tgagcattgg atagtattat
     6601 ccgggactgc tcgtgttaca aagggtgagc agatttatat ggtttctgaa aatgaatcaa
     6661 catttattcc tccggatact attcacgcgc tggaaaatcc cggaatgatc cccctgaagt
     6721 taattgagat tcaatcaggt acctatcttg gtgaggatga tattattcgt ttagaacaac
     6781 gttctggatt ttcgaaggag tggactaatg aacgtagtta ataatagccg tgatgttatt
     6841 cattcatcag gtattgtgtt tggaacgagt ggggctcgcg gtcttgtaaa agattttaca
     6901 cctcaggtat gtgctgcttt tacggtttca tttgttgccg ttatgcagga acatttttcc
     6961 tttgataccg tagcattggc aatagataat cgtccaagta gttatgggat ggctcaggcg
     7021 tgtgctgctg cattggcgga taaaggcgtt aactgtattt tttatggagt ggtaccaacc
     7081 ccagctttgg cctttcagtc tatgtctgac aatatgcctg cgataatggt tacgggaagt
     7141 catattccat tcgagcggaa cggcctcaag ttctatcgtc ccgatggtga aatcacgaaa
     7201 catgatgagg ctgcgatcct tagtgttgaa gatacgtgta gccatttaga gcttaaagaa
     7261 ctcatagttt cagaaatggc tgctgttaat tatatatctc gttatacatc tttattttct
     7321 actccattcc tgaaaaataa gcgtattggt atttacgaac attcaagcgc tgggcgtgat
     7381 ctttataagc ctttatttat tgcattgggg gctgaagtta ttagcttggg tagaagcgat
     7441 aactttgtac ctatagatac agaggctgta agcaaagagg atcgggaaaa agcacgctca
     7501 tgggctaaag agttcgattt agatgccata ttctcaacag atggggatgg tgatcgccct
     7561 cttattgctg atgaggcagg cgagtggttg agaggcgatg tattaggtct attatgttca
     7621 cttgcattgg atgcagaagc cgccgctatt cctgttagtt gtaatagcat aatttcttct
     7681 ggccgctttt ttaaacatgt taagcttacg aaaattggat cgccttatgt tatcgaagct
     7741 tttaatgaat tatcgcggag ttatagtcgt attgtcggct ttgaagccaa tggcggtttt
     7801 ttattaggaa gcgacatctg tattaacgag cagaatcttc atgccttacc aactcgtgat
     7861 gctgtattac cagcaataat gctgctttac aaaagtagga ataccagcat tagcgcttta
     7921 gtcaatgaac tcccaactcg ttacacccat tctgacagat tgcaggggat tacgactgat
     7981 aaaagtcaat ccttaattag tatgggcaga gaaaatctga gcaacctctt aagctatatt
     8041 ggcttggaga atgaaggtgc aatttctaca gatatgacag atggtatgcg aattacctta
     8101 cgtgatggat gtattgtgca tttgcgcgct tctggtaatg cacctgagtt acgctgctat
     8161 gcagaagcta atttattaaa tagagctcag gaacttgtaa atacaacgct tgctaatatt
     8221 aaaaaacgat gcttgccgta aaagaattga a
//
