LOCUS       NZ_CP129854            13379 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020S06-160 plasmid p2020S06-160-13k,
            complete sequence.
ACCESSION   NZ_CP129854
VERSION     NZ_CP129854.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281315
            Assembly: GCF_030490615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13379)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,107
            CDSs (total)                      :: 5,982
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 68 of 161
            Pseudo Genes (incomplete)         :: 99 of 161
            Pseudo Genes (internal stop)      :: 23 of 161
            Pseudo Genes (multiple problems)  :: 28 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129854.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020S06-160"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020S06-160-13k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            join(13114..13379,1..>282)
                     /locus_tag="QYQ35_RS30460"
                     /old_locus_tag="QYQ35_30460"
                     /pseudo=""
     CDS             join(13114..13379,1..>282)
                     /locus_tag="QYQ35_RS30460"
                     /old_locus_tag="QYQ35_30460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="group II intron reverse transcriptase/maturase"
     gene            complement(723..1016)
                     /locus_tag="QYQ35_RS30465"
                     /old_locus_tag="QYQ35_30465"
     CDS             complement(723..1016)
                     /locus_tag="QYQ35_RS30465"
                     /old_locus_tag="QYQ35_30465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301731752.1"
                     /translation="MQYQPFTASKIPAGTDENDHGKYSKTPLWISAMQAFAPDGRTLGG
                     QAASLRHMTGTTPGKQVQPNYPGNSLRSKTHTKVRAPPRLIWTQMDDGQRRI"
                     /name="hypothetical protein"
     gene            <3104..3510
                     /locus_tag="QYQ35_RS30470"
                     /old_locus_tag="QYQ35_30470"
                     /pseudo=""
     CDS             <3104..3510
                     /locus_tag="QYQ35_RS30470"
                     /old_locus_tag="QYQ35_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            3518..3763
                     /locus_tag="QYQ35_RS30475"
                     /old_locus_tag="QYQ35_30475"
                     /pseudo=""
     CDS             3518..3763
                     /locus_tag="QYQ35_RS30475"
                     /old_locus_tag="QYQ35_30475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523383.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /name="colicin E3-like toxin immunity protein"
     gene            3975..4726
                     /locus_tag="QYQ35_RS30480"
                     /old_locus_tag="QYQ35_30480"
                     /pseudo=""
     CDS             3975..4726
                     /locus_tag="QYQ35_RS30480"
                     /old_locus_tag="QYQ35_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            4727..4853
                     /locus_tag="QYQ35_RS30485"
                     /old_locus_tag="QYQ35_30485"
                     /pseudo=""
     CDS             4727..4853
                     /locus_tag="QYQ35_RS30485"
                     /old_locus_tag="QYQ35_30485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /name="colicin release lysis protein"
     gene            6406..8370
                     /locus_tag="QYQ35_RS30490"
                     /old_locus_tag="QYQ35_30490"
     CDS             6406..8370
                     /locus_tag="QYQ35_RS30490"
                     /old_locus_tag="QYQ35_30490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_301731753.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQAQKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8370..9101
                     /gene="mobC"
                     /locus_tag="QYQ35_RS30495"
                     /old_locus_tag="QYQ35_30495"
     CDS             8370..9101
                     /gene="mobC"
                     /locus_tag="QYQ35_RS30495"
                     /old_locus_tag="QYQ35_30495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9108..9638
                     /locus_tag="QYQ35_RS30500"
                     /old_locus_tag="QYQ35_30500"
     CDS             9108..9638
                     /locus_tag="QYQ35_RS30500"
                     /old_locus_tag="QYQ35_30500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9665..9844)
                     /locus_tag="QYQ35_RS30505"
                     /old_locus_tag="QYQ35_30505"
     CDS             complement(9665..9844)
                     /locus_tag="QYQ35_RS30505"
                     /old_locus_tag="QYQ35_30505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9870..10298)
                     /locus_tag="QYQ35_RS30510"
                     /old_locus_tag="QYQ35_30510"
     CDS             complement(9870..10298)
                     /locus_tag="QYQ35_RS30510"
                     /old_locus_tag="QYQ35_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(10462..10776)
                     /locus_tag="QYQ35_RS30515"
                     /old_locus_tag="QYQ35_30515"
     CDS             complement(10462..10776)
                     /locus_tag="QYQ35_RS30515"
                     /old_locus_tag="QYQ35_30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10764..11042)
                     /locus_tag="QYQ35_RS30520"
                     /old_locus_tag="QYQ35_30520"
     CDS             complement(10764..11042)
                     /locus_tag="QYQ35_RS30520"
                     /old_locus_tag="QYQ35_30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(11217..11582)
                     /locus_tag="QYQ35_RS30525"
                     /old_locus_tag="QYQ35_30525"
     CDS             complement(11217..11582)
                     /locus_tag="QYQ35_RS30525"
                     /old_locus_tag="QYQ35_30525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(11579..11950)
                     /gene="tolA"
                     /locus_tag="QYQ35_RS30530"
                     /old_locus_tag="QYQ35_30530"
     CDS             complement(11579..11950)
                     /gene="tolA"
                     /locus_tag="QYQ35_RS30530"
                     /old_locus_tag="QYQ35_30530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(12224..12469)
                     /locus_tag="QYQ35_RS30535"
                     /old_locus_tag="QYQ35_30535"
     CDS             complement(12224..12469)
                     /locus_tag="QYQ35_RS30535"
                     /old_locus_tag="QYQ35_30535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     Typing          8371..9098
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gagcaggaaa gcggccgccg gagtggatgg catcaggtgg atggactacg ccggaaacat
       61 gaagaacaac ataacagatc tgcaccggag gctacatcag ggcagctaca gggcgcagcc
      121 cggcaggcgt cactacatcc caaaagcgga tggaaaacaa cgcccgctcg gcatcgcctc
      181 gctggaggac aagatcgtcc agtatgcgct ggtgaaaatc ctgaacgcag tctatgaaaa
      241 cgactttatg gggttctcat acgccaggtt ttcagacccg gtctgaagtt cttcagagat
      301 tgcatgatgg attgcttgcc tggcagtggc atatccagga gctggtacgg cactaatgta
      361 ttacacgtgg gtactggatc cgtgcggcga gtatttgcca gtctggccgg tttttttctg
      421 gttatcggcc tgtttttcag ggtctgcttc cacgaatctg ggctgctaca ggtttcacag
      481 ggcatccagg ataaggcgac cggcgggtta atcaggccga tacgttactg gcgcgcatag
      541 ccgggataat cggactgagg ccattttggt gctggcaact cggtggaagg catccccaca
      601 gggtgcggtc atggtcagac tgcacggcaa acatataacc tcgcactacg cgtgccctgc
      661 gggatctgac cagggcgcat cagttgccgt ctgcgtttcc cttctggcaa ggtggtacat
      721 ggtcagatac gccgttgtcc atcgtccatc tgggtccaga tcaaacgtgg cggcgcccgg
      781 accttcgtat gcgttttgct gcgcagacta ttacctgggt agttcggctg cacctgttta
      841 ccgggagtag tacccgtcat gtgccggagt gaggccgctt ggccgccgag cgtcctgcca
      901 tcaggcgcga aagcttgcat ggctgaaatc cagaggggcg tttttgaata cttgccatgg
      961 tcattttcat ccgtgccggc gggaattttg gaggcggtga acggctgata ttgcatgctg
     1021 aatacaggag agccggatcg ccagccagcg gatcggatga cggcaactca gacgtccgcc
     1081 atcatttcgg ggagccggta tccgttaagg cgctggccat ttttcccgcg agcaggcctc
     1141 agggaaacat ctgcatttca ggacagtggt tctgggatgg cctgaagcgt agcactcgat
     1201 tttagccggt gcaacttccc caccatgtat aaattttcag gcggctgggt ggtggaatgc
     1261 ttacagatcc tcaggcctgc ccgggctgcg gcaagagcgc aggagccaaa gggtatcata
     1321 cgcatcgcgt tgatgacctt tcagtaaaca aactgttaca tgtactattt gactaccaaa
     1381 ggggacgcgt ggttcattta cataaacacg gcttatcgga gcgttttcac tgcccagaca
     1441 cccgccatgc agtctgcggc cggtgcataa atcggcgcag cgggatctgt tccgggcgcg
     1501 tatccggtaa cgggtacgcc ctgaccgcga catgttggct ttgttgcctg ttttaaatcc
     1561 cgtgagaccg gcgacggaaa acggaccact gaccgtcatg gagcggtcag aaagcgggca
     1621 atgctgacgt tgctggagac atacccgcag gtaccggcaa acaggcagca ggtggctggc
     1681 aggtgaacgg tacgtcgttg ccggtggccc tgaaatcagg acgtgaaggg gctgatcgac
     1741 catttcagct ggagcccgcc tggcttcgac gcatgagcgt cctgaatgga cagcgatatt
     1801 cataactata atccggcggg acctgttgtt ttaacggcgg cagctgtggt aatgtcatcg
     1861 gtttcagcgg cgatcatggc ggtgcctggt ggcagacaaa gatgctgtcg gccctgcgct
     1921 ggcgcttcgg tcttgcagcg ggatgtctag ttccggaggt ggcgtcatag cggcatctgc
     1981 tcgatggctt aaatatcagg tttccggcga acgttgtaac cgctggctga tctgctgcgc
     2041 atgcctgaag gggccgtttc agacctgctg tcgcgcacca tcgctgcgat ctacggtgtg
     2101 ctgaatgtct tagataatgc caaaagatgt tgccgaaaat gatgtcaaaa attacggtac
     2161 gggataacac gccgatccgc tggttctacg gcggcctcca gggcgctttt ttgggcggct
     2221 gccagaccag gagatccggg tggatcgtga gcgccaccgg gacaacggga ggatgtaagg
     2281 tgaataatga tactgcagca tagcggcgac agttcatcgc agggaggccg gccgagtgtc
     2341 caagtggtga acagtccccg accattgaac ctgcctgacg ggtatcgcac aagtgtcgat
     2401 tccgggtctg ggcgtggcat acggctgagg tatggagcga agcccgactg cggtgttgac
     2461 agcgccagcc attgagccac gaaatgttcg ctgaaaaccg ggtgccgacg tccggctggt
     2521 tttacagccg gaaggcaacg gatcccgcta gaggtgcgag tacctgcgtc cggccgacat
     2581 ggacccggac caaaagccgg tctgatgatt ttgcggggct tgacagatga tctctacgct
     2641 ggaacaggta gaacaacgtc aggaccaagc cagcgtcgcc agcaggcatg ggcgccgctc
     2701 accgggaaga cgagggctga ccctagcgct ggctgatgat aagagctgag ttccaagcgg
     2761 agctggttgc gcgaagatag ctggggattg caggcgtaca accggttaaa ggagccgcat
     2821 tcagtggagc gaaggctgct ccccgggcgc caagggctgc tggtcaggaa cagccgacaa
     2881 tacgccggtt acacagaggc cgtcaggcaa ataaagcatg tcacaggggc tgtaacacgc
     2941 ctgcacggga atatgcgctc gttcgacgag gacggcagaa agaacggttt acgctattgt
     3001 ttcagccgtc aagggtgtca gaatcgaatg agcagctgcc gaagcggctc cgtgcagtct
     3061 gcgcctaaac gcatgaaagc ggccttatcc ggagataagg ctggcactta ccggctggat
     3121 ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca gaaaatacgg ttaaatgagg
     3181 aaaagaacaa gcctcgcaag gggactaaag actacggcca ggattatcat ccagtaccta
     3241 aaacggaaga catacaaccg ccgggcgact tggcatcgtt aaagctggag gtgatcctaa
     3301 aacacctaaa caaggtgacg ctggtggtaa gcgggcccga tggtatggcg ataaaaaacg
     3361 taaaatttat gaatgggatt cccagcacgg tgagcttgac ccgggtatcg aagccagtga
     3421 tggcgatgca cctaggcgca tttgatccac aaaacgggta agcaggttaa agggccagat
     3481 ccaaaacgga acattaaaaa atatctttga gaggtaaata tggaagctta aattacgttt
     3541 gacatggttt gaccagcgag aaatctgaag agtcgcctgg cagaccggaa gagtattcta
     3601 aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta aaggataatc
     3661 ggcgaataat ggtgagtttg atttgaagaa agaatgggtt cctttactac agccattttt
     3721 taaaaatcaa ataaaacctc acatgtggat aattgttata tagcttttga ttaggtcggg
     3781 acggtaactg gccacaaaaa atgtatcaag gatgatttgc cggatactgc tcttccggtg
     3841 ttcaacgcga tgaatgccgg agtctttaaa tacagggaaa gtatatgaaa aaggtaaaga
     3901 ctatttttct ttttatattg agccgtctgc tggctttctt ggccgatgct accaacttgc
     3961 tgatttagtg gataatggtg tttttgacgg tgctcccgtg gcttcacgtc tccgtctgct
     4021 gtccctcctg ttccgctact gaaggcgtga tgcgtaatgg caaaagtacc tgccgggcat
     4081 caacgttatc tctgctctca ctgccgtaaa acatggcagc tcaccttcac ttatgccgct
     4141 tctcagctcg gtacacacca gaaaatcatt gatatggcta tgaacggcgt cggttgccgt
     4201 gccaccgcac gactatcctg ggcgtgggca tgttcaacac cattttgcgc catttaaaaa
     4261 actcaggccg cagtcagtaa ccagaaggat acagccgggc agtgatgtca aattgtctgt
     4321 gcggagatgg acgagcaatg gggctacgtc ggttcaaaat cacgtcagcg ctgggctgtt
     4381 ttacgcgtat gacaggatac gcagggcccg gtggtatcgc ccacgttttc ggtgaacgca
     4441 ctatggccac actggagcgt ctccctgagc ctgctgtcag tttttgatgt atggtggtat
     4501 ggatgacgga aaacggctgg ccgatgtatg agtcacgtct gaagggaaaa cttcacgtca
     4561 tcagtaagcg ttacacgcag cgaattgagc gacacaacct gaatctgaga cagcatctgg
     4621 caaggctggc cctgttttac aagtccctgt cgttctcaaa atcggtgccg cggctgcatg
     4681 acaaagtcat cggagcacta ttctgaacat aaaacactat cagtaaattg gagtcattac
     4741 cggctgtgtt cttctggccg catgtcaggc aaattatatc cgggatgttc agggcggaac
     4801 ggtggcactg catcgtcctt cggtctgaac tgacggggat cgcggttcag tagaaaagat
     4861 caaaggatct tcttgagatc ctttttgtct gccgccgtaa tcttttgccc tgtaaactga
     4921 aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacg
     4981 gtggtaactg gcacttcgca gagcacagca accaaatctg atccttccag tgtagcagaa
     5041 actttggcgc acacttcaag agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc
     5101 ccagttacca ctgctcacca gtgcgacgtt ttcgtgcaga cccgggtttg gactcaagtg
     5161 agaaagtgac cggataaggc gcagcaaatc gcggctgtgc gggcagcgtc ggccaaatcg
     5221 tgtccctgct ggtggatggc gaaccgatcc taaaacgttc cggagttccc cagtgtgtcg
     5281 agcttatcca ggcgtctccg cttcttccgc agggtgaaga caggtatccg gtaaacggca
     5341 ggttcctgaa cagcgagagc gccaaaaggc attgtccgtc ccgcagaaac tggtgatctt
     5401 ctcgtccggt cggttttcgc cggtactgtc agcctccagc attgagccgt tattcggctc
     5461 ccgagcagat gcaccggaca tcgaagccag cgtttatgtg aactcggtga caggagttct
     5521 tacatcgagc ctgatggaaa acagcgtcac cggagcgaca taaaagccgt tttgccagtc
     5581 aggcatgtat tacgtcccct gcttatccac ggaatctgtg ggctaacttt gtatgtgtcc
     5641 gcagcgcccg ccgcagtctc acgcccggag cgtagcgacc gagtgagcga ggaggcgcaa
     5701 tttccccggc acctgcaaga cggtcaccgg gaagcgctga tgtgggatat tgtcccctga
     5761 ttaagtcttt ctattccctg acggtcaatt atcaggatag gtgaaccatg tcggtttttc
     5821 gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga gaaatgcaag
     5881 tcatccccgc tggggcgtca ggctgacttc aggggaaaag acctgagctt acgctggctg
     5941 gacgccaccg acgcacggtc ccttttccag aatatcaaac gtggctggct ctgtaccggc
     6001 tggacgccgg ccacgccgga aattcacccg gcccccgtca ggctcacgcc tgaccaccca
     6061 caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg
     6121 gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca
     6181 ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc
     6241 gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac
     6301 cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc
     6361 tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat
     6421 tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc
     6481 tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc
     6541 ggtctggctg aactctgggc cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg
     6601 cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgtgccatt ttcggtgctg
     6661 gcctcgctgg attcacccgg gcggatggtc agggcacggc gacatgtctg gaactacgcg
     6721 tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt
     6781 atcgagagct ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc agattattac
     6841 ctgctgcagc cggctaacct gttcgggata gcctggtgtg ccggagggct tgtcgccggc
     6901 gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac
     6961 gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa
     7021 atgacagacg tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat
     7081 ttttccacgg agcaggccca gaaacagcag gcggtgtttc tgggtaagga tgagcagggc
     7141 cagccggtgc tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg
     7201 cccgggagcg gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc
     7261 ggggacgctg tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg
     7321 gcgcactgcc cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat
     7381 ctgttccgcg atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg
     7441 agtgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg
     7501 attgcggagc agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg
     7561 ttgccggagg ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc
     7621 gacctgagcg tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc
     7681 ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg
     7741 ctgtttgccc gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac
     7801 gcgagcatca tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg
     7861 gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc
     7921 ggtcagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg
     7981 ccgatccgct ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag
     8041 accggggaga tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg
     8101 gagcacatca gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac
     8161 accctgcaac acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg
     8221 gcgttcagca gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg
     8281 ctggcgaaaa cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa
     8341 gcggatgatg attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg
     8401 cgaagcggca ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag
     8461 agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg
     8521 tttaccgctt cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg
     8581 ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg
     8641 acgaggacga gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc
     8701 agctgccgca ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca
     8761 ccggctggcg gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg
     8821 cggagctgga cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc
     8881 ggtatcagga agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa
     8941 tctggtacat ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg
     9001 tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg
     9061 agaggctgtt cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca
     9121 tggtatttat tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg
     9181 agcatctgga agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg
     9241 tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca
     9301 acagcgacga aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca
     9361 aacccacagg aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg
     9421 agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga
     9481 ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc
     9541 tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca
     9601 aacccgcccg ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt
     9661 gctgttattc ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt
     9721 gaagccgttc gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca
     9781 acagcagcga ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg
     9841 tcatgttctc cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt
     9901 ttcttctgtg tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca
     9961 tcatcgtgct ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca
    10021 gcctgccgga gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt
    10081 ttttgtgtat tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt
    10141 ccgtatacac ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac
    10201 acggtaacct ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct
    10261 tgtgctgcct gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg
    10321 gtgtgaaagg tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg
    10381 ttcaggatgt ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa
    10441 ttgcgccccc gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg
    10501 atgcctgacg gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac
    10561 atagccactg tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac
    10621 cgggcggcca atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc
    10681 tccttttctg gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc
    10741 gcgctgtgcc agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca
    10801 gttccccagg tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc
    10861 tgatagtgct gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg
    10921 tcgatgtatt cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg
    10981 gccagcgttt tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac
    11041 ataaaagccc ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct
    11101 atttgccaga aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc
    11161 tgctacaggc tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac
    11221 tggcggcaac catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg
    11281 tcatttttca gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga
    11341 tatagccagt gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc
    11401 gacacgttgt ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg
    11461 atgcgttttg ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt
    11521 ggtatcgcat gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc
    11581 atggtctgaa atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg
    11641 gcggaatttt ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc
    11701 cttcagcaat ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca
    11761 tttttcccgc ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg
    11821 cagcgtagct actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg
    11881 tggaatgctt acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta
    11941 ttaaacgcat cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct
    12001 ttacggccac gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc
    12061 cgccatgcag ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg
    12121 cggttttccg ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac
    12181 cgtctgagcg gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg
    12241 gctgccaggt gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc
    12301 gcctggcttc acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta
    12361 ccgcctttgg cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg
    12421 cgtccgccag gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg
    12481 ggcgtctcag gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt
    12541 cagatgtgtc tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg
    12601 ttgccatgag aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt
    12661 tttgtgcctg aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag
    12721 gtaataatta tactgtgtat atgtacagtg tgcgccggga gaccggttag agatcaaggg
    12781 gtgaaagtcc ccgaccattg aaggaccagc aatccacaag gtccccgagt catgcgttgc
    12841 ataccgcgag gtatggggcg aagcgttgac aggggtgttg acaggccagc cattgagcca
    12901 cgaaatgtat attaaattac cgggtgccga cgttgtactg ttaacggaag gcaacatcat
    12961 agggtgcgat actgcgagtg ccacatggac ccggcggggt ctgagaccct ggcatgtcaa
    13021 tacgatctct acgcgggaac cgggagatct cccctctgac catctgccag tgtcggagat
    13081 ggcccgcacc gggaagacga ggagtcatag ccggtgatgt acggagagga gaagtcggac
    13141 tcgctcatag tagcggcgaa gcaggcgaac aacccgaaag gagcggagtc agtggagcga
    13201 aggagcgggg ccaaggggaa cgcggaacag ccacacatgc gccggacaca gagccgggaa
    13261 agcatgtcac agaggctgtc acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg
    13321 tttacagcat tgttccacct gctgacagtc gaagcactgg aagccgcatt cctctccct
//
