LOCUS       NZ_CP129846            12747 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020S05-051 plasmid p2020S05-051-12k,
            complete sequence.
ACCESSION   NZ_CP129846
VERSION     NZ_CP129846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281312
            Assembly: GCF_030490405.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12747)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:15:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,860
            CDSs (total)                      :: 5,736
            Genes (coding)                    :: 5,606
            CDSs (with protein)               :: 5,606
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 52 of 130
            Pseudo Genes (incomplete)         :: 78 of 130
            Pseudo Genes (internal stop)      :: 12 of 130
            Pseudo Genes (multiple problems)  :: 11 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129846.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020S05-051"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020S05-051-12k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            352..2316
                     /locus_tag="QYQ71_RS29230"
                     /old_locus_tag="QYQ71_29230"
     CDS             352..2316
                     /locus_tag="QYQ71_RS29230"
                     /old_locus_tag="QYQ71_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2316..3047
                     /gene="mobC"
                     /locus_tag="QYQ71_RS29235"
                     /old_locus_tag="QYQ71_29235"
     CDS             2316..3047
                     /gene="mobC"
                     /locus_tag="QYQ71_RS29235"
                     /old_locus_tag="QYQ71_29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3054..3584
                     /locus_tag="QYQ71_RS29240"
                     /old_locus_tag="QYQ71_29240"
     CDS             3054..3584
                     /locus_tag="QYQ71_RS29240"
                     /old_locus_tag="QYQ71_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3611..3790)
                     /locus_tag="QYQ71_RS29245"
                     /old_locus_tag="QYQ71_29245"
     CDS             complement(3611..3790)
                     /locus_tag="QYQ71_RS29245"
                     /old_locus_tag="QYQ71_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3816..4244)
                     /locus_tag="QYQ71_RS29250"
                     /old_locus_tag="QYQ71_29250"
     CDS             complement(3816..4244)
                     /locus_tag="QYQ71_RS29250"
                     /old_locus_tag="QYQ71_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4408..4722)
                     /locus_tag="QYQ71_RS29255"
                     /old_locus_tag="QYQ71_29255"
     CDS             complement(4408..4722)
                     /locus_tag="QYQ71_RS29255"
                     /old_locus_tag="QYQ71_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4710..4988)
                     /locus_tag="QYQ71_RS29260"
                     /old_locus_tag="QYQ71_29260"
     CDS             complement(4710..4988)
                     /locus_tag="QYQ71_RS29260"
                     /old_locus_tag="QYQ71_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5163..5528)
                     /locus_tag="QYQ71_RS29265"
                     /old_locus_tag="QYQ71_29265"
     CDS             complement(5163..5528)
                     /locus_tag="QYQ71_RS29265"
                     /old_locus_tag="QYQ71_29265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5525..5896)
                     /gene="tolA"
                     /locus_tag="QYQ71_RS29270"
                     /old_locus_tag="QYQ71_29270"
     CDS             complement(5525..5896)
                     /gene="tolA"
                     /locus_tag="QYQ71_RS29270"
                     /old_locus_tag="QYQ71_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6170..6415)
                     /locus_tag="QYQ71_RS29275"
                     /old_locus_tag="QYQ71_29275"
     CDS             complement(6170..6415)
                     /locus_tag="QYQ71_RS29275"
                     /old_locus_tag="QYQ71_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7060..8547
                     /gene="ltrA"
                     /locus_tag="QYQ71_RS29280"
                     /old_locus_tag="QYQ71_29280"
     CDS             7060..8547
                     /gene="ltrA"
                     /locus_tag="QYQ71_RS29280"
                     /old_locus_tag="QYQ71_29280"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            8652..10337
                     /locus_tag="QYQ71_RS29285"
                     /old_locus_tag="QYQ71_29285"
     CDS             8652..10337
                     /locus_tag="QYQ71_RS29285"
                     /old_locus_tag="QYQ71_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10347..10604
                     /locus_tag="QYQ71_RS29290"
                     /old_locus_tag="QYQ71_29290"
     CDS             10347..10604
                     /locus_tag="QYQ71_RS29290"
                     /old_locus_tag="QYQ71_29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            10798..11495
                     /locus_tag="QYQ71_RS29295"
                     /old_locus_tag="QYQ71_29295"
     CDS             join(10798..11047,11047..11495)
                     /locus_tag="QYQ71_RS29295"
                     /old_locus_tag="QYQ71_29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            11496..11615
                     /locus_tag="QYQ71_RS29300"
                     /old_locus_tag="QYQ71_29300"
     CDS             11496..11615
                     /locus_tag="QYQ71_RS29300"
                     /old_locus_tag="QYQ71_29300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_194315302.1"
                     /translation="MGVITGFLLAACQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     BGC             3652..12747
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11719..12297)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2317..3044
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt
       61 ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag
      121 accgcactgg ggtgccgcct tttcctctca cacgctgggg cgtgctcgtt gttcctccgg
      181 ggctgcgcct tttccgcatg gggcggaaag aggctggcct ccgcggtgaa agctgaacgc
      241 aatcaccacg gacgtgaccg ccgccgccat gccttggggc atgtccgcgg cttgtgcagc
      301 gggggctgcg catcacgccg gtaaacccgt cgtctgaagg agagagattg catgtttaat
      361 acggattcgc ttgcctggca gtggcatatc ccggagctgg cccggcagat ttacccgtgg
      421 gtcagctggc tgtaccggga gtattacagt ctggccggtt tttttctggt tatcggcctg
      481 tttttcggtc tggctgaact ctgggccggc cgaatgcgtt ttgtggggct gagccataag
      541 gcgcggcggg agtatgccga tcgctataag cgcgataata accggactgt gccattttcg
      601 gtgctggcct cgctggattc acccgggcgg atggtcaggg cacggcgaca tgtctggaac
      661 tacgcgtact ggctgaccgg cgctgccggt ctggcccttc tgggggggct ggttgtcgcc
      721 gtgtttatcg agagctgggg agaaaccgtc ctgcgcccgg acctctatgc gaacgcagat
      781 tattacctgc tgcagccggc taacctgttc gggatagcct ggtgtgccgg agggcttgtc
      841 gccggcgtcc ttctggcgcg gctgctggcg ttcctgctgc ttgatggtca ttttgcggcc
      901 gctgacgagg cggtgaacgc gaaactgaat caggagagcc ggcgcagcag ccagcggacc
      961 ggagaaatga cagacgtccg ccacctgcat ttcggggagc cggtaccggt taacgcgctg
     1021 gccgattttt ccacggagca ggcccggaaa cagcaggcgg tgtttctggg taaggatgag
     1081 cagggccagc cggtgctggt ccctcgtgat acctggcgca agaccaatat tcagatcctc
     1141 ggcctgcccg ggagcggcaa gagcgcgatg gccaccaacg cgctcattcg ctgcgtacgc
     1201 gatttcgggg acgctgtggt gtacttcgac cccaaagggg acgcgtgggc gccgcacgtc
     1261 ttccgggcgc actgcccgga cttcaccctg ctggatctgc ggccggggaa accggcgcag
     1321 ctgaatctgt tccgcgatct cgaccagtac gccctgaaga atctgctggt tgccgggttt
     1381 aacctgagtg agaccggcga cgtggcggac cactaccgca tcagcgagca gaaagcggcg
     1441 aagctgattg cggagcagtt cccgcagggg gcaaacattc agcaggtgct ggcggcggcg
     1501 tacgcgttgc cggaggccct gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac
     1561 gtggccgacc tgagcgtcct gcagacggac agcggtatcg atgtggcggg gataatcaac
     1621 ggcggcggct gcctgtacgt catcggctcg atggacgatg aggcggtgat ccgggtgcag
     1681 aagatgctgt ttgcccgctg cgcgcaaatc atcattgcgc gggatgagtt ccggaggtgg
     1741 ccgcacgcga gcatcatgct cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac
     1801 gcgctgggga cgctgcgcag ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc
     1861 gacttcggtc agtgcgggca ggatctgccc gctgattttg tgaaaaccac ggtactggat
     1921 aacacgccga tccgctggtt ctatcgcgcg gccagccagg agtccgcgca gtgggcggcg
     1981 ggccagaccg gggagatccg ggtggacgtg gagcgccgcc gggccagccg ggaggcgggg
     2041 aacgtggagc acatcagcgg cgacagcttc atccagaagg aggcgcggcc gctgtttgac
     2101 gtgaacaccc tgcaacacct gcctgacggg ttcgcggtga tgaccgggct gggcgtggcg
     2161 cggctggcgt tcagcagccc gctgcgggtt gagcgccggg aaatttcgct gaaaaccttc
     2221 ccggtgctgg cgaaaacgga cccgctggcg gagtacctgc ctccggccga cggcggcgga
     2281 ccaaaagcgg atgatgattt tgcggggctt tactgatgac actggagcag tacaacgtca
     2341 gccacgcgaa gcggcaggcg cggaacgcgg agaaaacacg gctgaccctg cgctggctgc
     2401 gggaagagct gtgttccacg gcggagctgg ttgcgcgacg gctggggatt gcggcggtac
     2461 agccggttta ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc cgggcgaaat
     2521 acccggttga cggccgtcag gtcagcgtgt gggggctgac gccgcacggg gtggcgttct
     2581 cgttcgacga ggacgagccg ctgacggacg ttattccgtt tcagccgtca agggtgtcgg
     2641 cggcgcagct gccgcaccgg ctggccgtgc agtccctgcg cctctccatg gagggccggg
     2701 gggccaccgg ctggcggtac ctgcaccgta tggccctgaa agggatgaaa gtgccggacg
     2761 cgctggcgga gctggacggc agaacggtgg catttgaggt agagcgcacc gtcaagagcc
     2821 gcaggcggta tcaggaagtg gtgtccgggt atctgttcaa ccgccgggcg aacggcatcg
     2881 atgaaatctg gtacatctgc cctgaccggc cgacgcttgt ccgggtacag cgggcgattc
     2941 tggcggtgga tgaaatcgtg aatccacaga ccggcgaggc ccgcaaaacg gtggagctgg
     3001 accgggagag gctgttcgcg tgctttaaat ttatgacaac ggagtgaggg attatggcgc
     3061 tgtacatggt atttattgag gacgtggaag acaaagacgg cgagcgctgc attatgccaa
     3121 ccagcgagca tctggaagac atggtggtgc tcgcctggca gaccggggcg tatgttttct
     3181 gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg tatgacgagc
     3241 ggctcaacag cgacgaaaaa gccacgtact ttttcaccgg tgaaaatccg caatgggaac
     3301 gggacaaacc cacaggaatg aagctgtgtg attacctcta tcgaaaggtg gagcggttcg
     3361 ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag cagattaacg
     3421 cgatgattga ggtggtcagc gagcggcacg gggaaagcgc ggtgaacgag aagctgaaag
     3481 cggagctgat gcgggaatat aacagccgct attttaacgt gccggtgcct gaactgccgg
     3541 aggacaaacc cgcccgccgg tggtggccgt tcggcaaaaa ataacgccag caccggcatg
     3601 gccggtgctg ttattcttcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat
     3661 cctcgtgaag ccgttcgcac atgtcggccg gggtatccat gtccagctcg ttcagctttt
     3721 ccagcaacag cagcgactga tccttaatga atttagccat attgagggca gttttttcct
     3781 gcttcgtcat gttctccatt cccttcggta agttctcagc taaaccagcc ggaccagaag
     3841 cgttttttct tctgtgtcag ttctgttgtc aatctgtcca cttcggcctg gagtttccgg
     3901 cgctcatcat cgtgctggcg gcgttcttct tcccgggatg ttttatcttc cagcatcagg
     3961 gtgacagcct gccggagatc ggccagttcc ttttgcattg ccagaagctg ttcctgtggg
     4021 aaattttttt gtgtattttc acggtgtgaa atgccgggaa tttgtttctc agggatattc
     4081 acatttccgt atacacggat cagttctgac acatccacga cagggttgtt tttcccgtca
     4141 cgtgacacgg taacctttcc ctgttttaca tgattgtata gcgttcttct ggtaatgccc
     4201 gcggcttgtg ctgcctgtga aaggttgagc aatgttttcg ccactgtata cccctgattt
     4261 tatcgggtgt gaaaggtgtg taatttctca cggtaaatgt atcgctgaat tttacccgtt
     4321 tttccgttca ggatgtggtt ttctgacgcg ctgcgcttgt caaaccggcg acggccacgc
     4381 aatgaattgc gcccccgtca tcggttatta gaggctgtct gcccccggat acccggattc
     4441 tttctgatgc ctgacggtga cgatcacaat ctggtccatt tcccggtcgt ggcggtagag
     4501 catcacatag ccactgtcgc caaacccgat caccagttcc ttatattcca gcggcagaaa
     4561 cggcaccggg cggccaatgt ctggcaatgt tttcagttgc tggatagccc ggacgatcac
     4621 ctcacctcct tttctggctg ccagccgatt tttggttttg agaaagtcct gaaggcgttg
     4681 taaatcgcgc tgtgccagcg cggaaatcgt tacctgtggc atggcggagc atcctgctcg
     4741 ttctcagttc cccaggtact tatccaggct tccgcttctt ccgcagtaag gtgcagaccc
     4801 gtttcctgat agtgctgcca tgcggcctgt ccgtcccgca ggtactggct gcgcttctct
     4861 tcccggtcga tgtattcggt gattgcctcc agcattagcg cgtgcgctga gcggtgccgg
     4921 tcatcggcca gcgttttcag ccggtcctta agttcttcat cgagcctgat ggatgttgct
     4981 gatgacataa aagccccttt gtagtcatat gtattacaca tgactacttt aatccgtggc
     5041 ggcgctattt gccagaaata cggcttttgc tgcccatttt tcagcttcgc ttccactaat
     5101 cagggctgct acaggctcac tcatcatcca ggactattgc agcctgcggg ttaaaggacc
     5161 tgttactggc ggcaaccatc atcattgagc ttttgggtgg tgactggtcg cggatcctgt
     5221 aagccgtcat ttttcagact gaattcaaaa tttataacct gtcgcattgc cgggtgccct
     5281 gcgggatata gccagtgcct cattgccgtc agcgtttccc gaaaaaaaag gtgacagccg
     5341 gtaagcgaca cgttgtccac tcgtccatcg tccagaatat caaacgtggc ggtcacctga
     5401 ccttcgatgc gttttgctgc ggcctgtacc gggtaggttg gctgcagtgt ggttacgggt
     5461 tttagtggta tcgcatgtac cggagtgagg aacatggccg ccagcactgt cagagtaaaa
     5521 gctttcatgg tctgaaatcc agaggggcgt ttttgaatat ttgccaggta ttttcatccg
     5581 gtgccggcgg aattttggcg tgggtaacgg ctgatattgc tgctttacag agtttcggat
     5641 ctcctccttc agcaatggcg ctgttcagag tgccatcacg ttgtatggat atccgcacgg
     5701 tgcacatttt tcccgcatag gcctctggtt caaacatctg catttcaaca gcggtcttga
     5761 tggccgcagc gtagctactg attttagatg acttatccag agtataaatt ttcggcggct
     5821 gggtggtgga atgcttacag gccgtcagga gtaaggggct gcacaatgcc agcaggataa
     5881 agggtattaa acgcatcggg ttgatgacct gtcagtaaaa aatgttacat gtatatttgt
     5941 cactctttac ggccacgttc atttacataa acacggctta tcgtatcgtt tttacaccaa
     6001 acggcccgcc atgcagttgc gtgcataggc atgcagcggg ttacaattta ccgggcgcgt
     6061 ttccggcggt tttccgggtg gtttgttgcc tgttttatcc cgtagccgcc ggaaacgccc
     6121 tcagaccgtc tgagcggtgc gggcaatgcg acgttatggt aaatacccct taccggcggc
     6181 gctgtggctg ccaggtgaag gtggtttccg tagcctcgta tttctcccga cgcatttcct
     6241 gcgcccgcct ggcttcacgt tcgtcctgaa tggcacgaag caccctttcc cgcacctgtt
     6301 gttttaccgc ctttggcagc gttttgttcc ctttcaggcc catggccagt gcctgagcga
     6361 caaaggcgtc cgccaggcgc ttttcttcag cgggagtcag tttcaggtgc gtcatagtgg
     6421 cctccgggcg tctcaggttt cccgaacagt ttaaccgcag gtcctctcct catgccagtt
     6481 agctttcaga tgtgtctata ccatctgaaa tgtaagtgtg atgaatgtct taaataataa
     6541 aatttgttgc catgagaaat tttcgggaaa agacgcgatc tgcttacagg tggctccggg
     6601 cgcttttttg tgcctgaaga tgagaactaa tctcatatat tgacacctga aaacgggagg
     6661 agtaaggtaa taattatact gtgtatatgt acagtgtgcg ccgggagacc ggttagagat
     6721 caaggggtga aagtccccga ccattgaagg accagcaatc cacaaggtcc ccgagtcatg
     6781 cgttgcatac cgcgaggtat ggggcgaagc gttgacaggg gtgttgacag gccagccatt
     6841 gagccacgaa atgtatatta aattaccggg tgccgacgtt gtactgttaa cggaaggcaa
     6901 catcataggg tgcgatactg cgagtgccac atggacccgg cggggtctga gaccctggca
     6961 tgtcaatacg atctctacgc gggaaccggg agatctcccc tctgaccatc tgccagtgtc
     7021 ggagatggcc cgcaccggga agacgaggag tcatagccgg tgatgtacgg agaggagaag
     7081 tcggactcgc tcatagtagc ggcgaagcag gcgaacaacc cgaaaggagc ggagtcagtg
     7141 gagcgaagga gcggggccaa ggggaacgcg gaacagccac acatgcgccg gacacagagc
     7201 cgggaaagca tgtcacagag gctgtcacgc gtgcgggaag ctgcgaagca gcggaagaaa
     7261 gaacggttta cagcattgtt ccacctgctg acagtcgaag cactggaagc cgcattcctc
     7321 tccctgagca ggaaagcggc cgccggagtg gatggcatca ggtggatgga ctacgccgga
     7381 aacatgaaga acaacataac agatctgcac cggaggctac atcagggcag ctacagggcg
     7441 cagcccggca ggcgtcacta catcccaaaa gcggatggaa aacaacgccc gctcggcatc
     7501 gcctcgctgg aggacaagat cgtccagtat gcgctggtga aaatcctgaa cgcagtctat
     7561 gaaaacgact ttatggggtt ctcatacggg ttcagacccg ggcgaagcca gcacgatgca
     7621 ctggacgcac tggccacagg gctggtacgc actaacgtaa actgggtact ggatgccgac
     7681 atcagtcagt tcttcgacag ggtgagccac gaatggctga tcaggttcac agagcatcgg
     7741 atcggcgacc ggagggtaat caggctcata cgtaagtggc tcacagccgg gacgtcggag
     7801 gagggtcaat ggcgagcaac ggaggaaggc accccacagg gtgcggtcat ctcaccgctg
     7861 ctggcaaaca tatacctcca ctacgtcttc gatctgtggg cgcatcagtg gcgacgtcgc
     7921 tatgccacag gcaatgtggt aatggtcaga tacgccgatg acatcgtcat cgggttcgac
     7981 aaacgatacg atgcccggcg cttccgtata gccatgcagc gcagactgag ggagttcgga
     8041 ctcacggttc acccggagaa aacccgtctg atggagttcg gccgcttcgc tgccgaaaac
     8101 cgtgccatca ggggaaaagg caaaccagaa acgttcaact tcctcgggtt cacgcacatc
     8161 agcgggaaag atcgcaacgg caggttcatg ctgatacgaa agacccgccg ggatcggatg
     8221 acggcaactc tgaaagccat caaagacggt ctgcgaaggc gctggcatta ctcaatcccc
     8281 gaacagggaa aatggctcag gagagtggtt cagggatacc tgaactatca ctcggtaccg
     8341 ggcaacttcc ccaccatgca gaagttcagg acacacgtaa caaacctctg gcgccgggcg
     8401 ctcaggcgca ggagccagaa ggatgatacg acctggacga aagcaaacaa actggcagcc
     8461 gcatggctac caagggttcg ggttcttcat ccatggcctg tggagcggtt caccgccaga
     8521 cacccgaggc aggagcccgg tgcgtaaatc gcgcacgccg ggatctgtgc ggggggtatc
     8581 cggtaacggg tatccctacc gcgacatgtt ggctttaatt tattgtttta aatgtcaaag
     8641 aggaaaacgg tatgagtggt ggagacggtc gaggtccggg taattcaggt ctgggacata
     8701 atggtggtca ggccagtggg aatgtgaacg gtacgtctgg taaaggtggc ccttcatcag
     8761 gtggtggtac ggatccaaac agcgggccgg gctggggtac gacgcatacg ccgaatggag
     8821 atattcataa ctataatccg ggggagtttg gtaacggcgg gagtaaaccc ggtggtaatg
     8881 gtggtaacag cggcaatcat agcggtagct ctggtggcgg acagtcttcg gccactgcga
     8941 tggccttcgg tctgcctgcc ctggctactc cgggggctga aggactggct ttatcagttt
     9001 ccggcgatgc gttgtcggcc gccgttgctg atgtgctggc tgccctgaag gggccgttta
     9061 agtttggtct gtgggggatt gcgatctacg gtgtgctgcc ttctgagatt gcaaaagatg
     9121 atccgaaaat gatgtcaaaa attatgacgt cattaccggc cgatgcggtg acggagactc
     9181 cggcaagtac tttaccactg gaccaggcga cggttcgtgt cagacaacgg gttgtggatg
     9241 tggtgaagga tgagcggcag catattgcgg ttgtcgcagg tcggccaatg agtgtccctg
     9301 tggtggatgc gaaaccgaca aaacgtccgg gggtattcag tgtgtcgatt ccgggtctcc
     9361 cgtctctgca ggtgagcgta ccgaaaggtg ttccgacagc gaaagccccg ccaaaaggca
     9421 ttgttgctga aaaaggtgat tcacgtccgg ctggttttac agccggtggt aactcccgtg
     9481 aggccgttat tcgtttcccg aaagagaccg gacagaagcc ggtttatgtg tcggtgacag
     9541 atgttcttac cccggcacag gtaaaacagc gtcaggagga agaaaagcgt cgccagcagg
     9601 catgggacgc cgctcatccg gaagaggggc tgaaaagaga ctatgataaa gcgaaagctg
     9661 agctggatgc cgaagataaa aatattgcga ccttaaacag ccgcattgca tcgacagaga
     9721 aggcgctccc cggtgcaagg gctgctgttc aggaagccga taaaaaggta aaagaggcag
     9781 aggcaaataa agatgatttt gtgacttata acccgcctca tgaatatggc tccgggtggc
     9841 aggatcaggt tcgctatctt gataaggata ttcagaatca gaatgagaaa ttaaaagcgg
     9901 ctcaggcatc tttaaacgca atgaatgaat ccttatccag agataaggct gcacttaccg
     9961 gggcgatgga gagccggaaa caaaaggaga aaaaagcgaa ggatgcagaa aataagttaa
    10021 atgaggaaaa gaacaagcct cgcaagggga ctaaagacta cggccatgat tatcatccag
    10081 tacctaaaac agaagacata aagggacttg gagagttaaa gcgaggtgat cctaaaacac
    10141 ctaaacaagg tggtggtggt aagcgggccc gatggtatgg cgataaaaaa cgtaaaattt
    10201 atgaatggga ttcccagcac ggtgagcttg aagggtatcg tgccagtgat ggcgaacacc
    10261 taggcgcatt tgatcccaaa acgggtaagc aggttaaagg gccagatcca aaacggaaca
    10321 ttaaaaaata tctttgagag gtaaatatgg ggcttaaatt acgtttgaca tggtttgata
    10381 agaaaactga agagtttaaa ggggaagagt attctaaaga ctttggtgac gatggttctg
    10441 tcattgaaag cttgggaatg cctttaaagg ataatattaa taatggtgag tttgatttga
    10501 agaaagaatg ggttccttta ctacagccat tttttaaaaa tcaaataaaa cctgatgtgg
    10561 ataattgtta tatagctttt gattatcggg acggtaactg gtaacaaaaa attatcaagg
    10621 atgatttgaa atactgactt ccggtgttca gggatgaatg ccggagtctt taaatacagg
    10681 gaaagtatat gaaaaaggta aagactattt ttctttttat attgattgtt gctggctttc
    10741 ttggtgatgc taccaacttg ctgatttagt gtataatggt gtttttgagg tgctcccgtg
    10801 gcttcagtct ccgtctgctg tccctcctgt tccgctactg aaggcgtgat gcgtaatggc
    10861 aaaagtactg ccgggcatca acgttatctc tgctctcact gccgtaaaac atggcagctc
    10921 accttcactt atgccgcttc tcagcccggt acacaccaga aaatcattga tatggctatg
    10981 aacggcgtcg gttgccgtgc caccgcacga ctaatgggcg tgggcctcaa caccattttg
    11041 cgccatttaa aaaactcagg ccgcagtcag taacctcccg gatacagccg ggcagtgatg
    11101 tcattgtctg tgcggagatg gacgagcaat ggggctacgt cggtgcaaaa tcacgtcagc
    11161 gctggctgtt ttacgcgtat gacaggatac gcagggcggt ggtagcccac gttttcggtg
    11221 aacgcactat ggccacactg gagcgtcttc tgagcctgct gtcagttttt gacgtggtgg
    11281 tatggatgac ggacggctgg ccgatgtatg agtcacgtct gaagggaaaa ctgcacgtca
    11341 tcagtaagcg ttacacgcag cgaattgagc gacacaacct gaatctgaga cagcatctgg
    11401 caaggctggg caggaagtcc ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
    11461 tcgggcacta tctgaacata aaacactatc agtaaattgg agtcattacc ggctttcttc
    11521 tggccgcatg tcaggcaaat tatatccggg atgttcaggg cggaacggtg gcaccatcgt
    11581 cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga
    11641 tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg
    11701 tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga
    11761 gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca
    11821 accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag
    11881 tggcgttttg cgtgctttcc cgggttggac tcaagtgaac agttaccgga taaggcgcag
    11941 cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc
    12001 gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca agggagaacg
    12061 gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgacct
    12121 accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg
    12181 gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc
    12241 aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg
    12301 ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg cagcgcccgc
    12361 cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat ttccccggca
    12421 cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt aagtctttct
    12481 attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc tgaacatttt
    12541 acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc atccccgctg
    12601 gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc caccgacgca
    12661 cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc agaaattcac
    12721 ccggcccccg tcaggctcac gcctgac
//
