LOCUS       NZ_CP129818            11981 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020N06-159 plasmid p2020N06-159-11k,
            complete sequence.
ACCESSION   NZ_CP129818
VERSION     NZ_CP129818.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281319
            Assembly: GCF_030490145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11981)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:04:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,812
            CDSs (total)                      :: 5,688
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 58 of 146
            Pseudo Genes (incomplete)         :: 87 of 146
            Pseudo Genes (internal stop)      :: 11 of 146
            Pseudo Genes (multiple problems)  :: 10 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129818.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020N06-159"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020N06-159-11k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            complement(join(11936..11981,1..200))
                     /locus_tag="QYQ53_RS28995"
                     /old_locus_tag="QYQ53_28995"
     CDS             complement(join(11936..11981,1..200))
                     /locus_tag="QYQ53_RS28995"
                     /old_locus_tag="QYQ53_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            845..2342
                     /gene="ltrA"
                     /locus_tag="QYQ53_RS29000"
                     /old_locus_tag="QYQ53_29000"
                     /pseudo=""
     CDS             845..2342
                     /gene="ltrA"
                     /locus_tag="QYQ53_RS29000"
                     /old_locus_tag="QYQ53_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="ltrA"
     gene            2447..4132
                     /locus_tag="QYQ53_RS29005"
                     /old_locus_tag="QYQ53_29005"
     CDS             2447..4132
                     /locus_tag="QYQ53_RS29005"
                     /old_locus_tag="QYQ53_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4142..4399
                     /locus_tag="QYQ53_RS29010"
                     /old_locus_tag="QYQ53_29010"
     CDS             4142..4399
                     /locus_tag="QYQ53_RS29010"
                     /old_locus_tag="QYQ53_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            4484..4633
                     /locus_tag="QYQ53_RS29015"
                     /old_locus_tag="QYQ53_29015"
     CDS             4484..4633
                     /locus_tag="QYQ53_RS29015"
                     /old_locus_tag="QYQ53_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6117..8081
                     /locus_tag="QYQ53_RS29020"
                     /old_locus_tag="QYQ53_29020"
     CDS             6117..8081
                     /locus_tag="QYQ53_RS29020"
                     /old_locus_tag="QYQ53_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8081..8812
                     /gene="mobC"
                     /locus_tag="QYQ53_RS29025"
                     /old_locus_tag="QYQ53_29025"
     CDS             8081..8812
                     /gene="mobC"
                     /locus_tag="QYQ53_RS29025"
                     /old_locus_tag="QYQ53_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8819..9349
                     /locus_tag="QYQ53_RS29030"
                     /old_locus_tag="QYQ53_29030"
     CDS             8819..9349
                     /locus_tag="QYQ53_RS29030"
                     /old_locus_tag="QYQ53_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9376..9555)
                     /locus_tag="QYQ53_RS29035"
                     /old_locus_tag="QYQ53_29035"
     CDS             complement(9376..9555)
                     /locus_tag="QYQ53_RS29035"
                     /old_locus_tag="QYQ53_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9581..10009)
                     /locus_tag="QYQ53_RS29040"
                     /old_locus_tag="QYQ53_29040"
     CDS             complement(9581..10009)
                     /locus_tag="QYQ53_RS29040"
                     /old_locus_tag="QYQ53_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(10174..10488)
                     /locus_tag="QYQ53_RS29045"
                     /old_locus_tag="QYQ53_29045"
     CDS             complement(10174..10488)
                     /locus_tag="QYQ53_RS29045"
                     /old_locus_tag="QYQ53_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10476..10754)
                     /locus_tag="QYQ53_RS29050"
                     /old_locus_tag="QYQ53_29050"
     CDS             complement(10476..10754)
                     /locus_tag="QYQ53_RS29050"
                     /old_locus_tag="QYQ53_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(10929..11294)
                     /locus_tag="QYQ53_RS29055"
                     /old_locus_tag="QYQ53_29055"
     CDS             complement(10929..11294)
                     /locus_tag="QYQ53_RS29055"
                     /old_locus_tag="QYQ53_29055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(11291..11662)
                     /gene="tolA"
                     /locus_tag="QYQ53_RS29060"
                     /old_locus_tag="QYQ53_29060"
     CDS             complement(11291..11662)
                     /gene="tolA"
                     /locus_tag="QYQ53_RS29060"
                     /old_locus_tag="QYQ53_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     BGC             1..9132
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4737..5315)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8082..8809
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tcgtatttct cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca
       61 cgaagcaccc tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc
      121 aggcccatgg ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga
      181 gtcagtttca ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac
      241 cgcaggtcct ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa
      301 gtgtgatgaa tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg
      361 cgatctgctt acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca
      421 tatattgaca cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt
      481 gtgcgccggg agaccggtta gagatcaagg ggtgaaagtc cccgaccatt gaaggaccag
      541 caatccacaa ggtccccgag tcatgcgttg cataccgcga ggtatggggc gaagcgttga
      601 caggggtgtt gacaggccag ccattgagcc acgaaatgta tattaaatta ccgggtgccg
      661 acgttgtact gttaacggaa ggcaacatca tagggtgcga tactgcgagt gccacatgga
      721 cccggcgggg tctgagaccc tggcatgtca atacgatctc tacgcgggaa ccgggagatc
      781 tcccctctga ccatctgcca gtgtcggaga tggcccgcac cgggaagacg aggagtcata
      841 gccggtgatg tacggagagg agaagtcgga ctcgctcata gtagcggcga agcaggcgaa
      901 caacccgaaa ggagcggagt cagtggagcg aaggagcggg gccaagggga acgcggaaca
      961 gccacacatg cgccggacac agagccggga aagcatgtca cagaggctgt cacgcgtgcg
     1021 ggaagctgcg aagcagcgga agaaagaacg gtttacagca ttgttccacc tgctgacagt
     1081 cgaagcactg gaagccgcat tcctctccct gagcaggaaa gcggccgccg gagtggatgg
     1141 catcaggtgg atggactacg ccggaaacat gaagaacaac ataacagatc tgcaccggag
     1201 gctacatcag ggcagctaca gggcgcagcc cggcaggcgt cactacatcc caaaagcgga
     1261 tggaaaacaa cgcccgctcg gcatcgcctc gctggaggac aagatcgtcc agtatgcgct
     1321 ggtgaaaatc ctgaacgcag tctatgaaaa cgactttatg gggttctcat acgggttcag
     1381 acccgggcga agccagcacg atgcactgga cgcactggcc acagggctgg tacgcactaa
     1441 cgtaaactgg gtactggatg ccgacatcag tcagttcttc gacagggtga gccacgaatg
     1501 gctgatcagg ttcacagagc atcggatcgg cgaccggagg gtaatcaggc tcatacgtaa
     1561 gtggctcaca gccgggacgt cggaggaggg tcaatggcga gcaacggagg aaggcacccc
     1621 acagggtgcg gtcatctcac cgctgctggc aaacatatac ctccactacg tcttcgatct
     1681 gtgggcgcat cagtggcgac gtcgctatgc cacaggcaat gtggtaatgg tcagatacgc
     1741 cgatgacatc gtcatcgggt tcgacaaacg atacgatgcc cggcgcttcc gtatagccat
     1801 gcagcgcaga ctgagggagt tcggactcac ggttcacccg gagaaaaccc gtctgatgga
     1861 gttcggccgc ttcgctgccg aaaaccgtgc catcagggga aaaggcaaac cagaaccaac
     1921 tcctcgttca ccacttcctc gggttaacgc acatcagcgg gaaagatcgc aacggcaggt
     1981 tcatgctgat acgaaagacc cgccgggatc ggatgacggc aactctgaaa gccatcaaag
     2041 acggtctgcg aaggcgctgg cattactcaa tccccgaaca gggaaaatgg ctcaggagag
     2101 tggttcaggg atacctgaac tatcactcgg taccgggcaa cttccccacc atgcagaagt
     2161 tcaggacaca cgtaacaaac ctctggcgcc gggcgctcag gcgcaggagc cagaaggatg
     2221 atacgacctg gacgaaagca aacaaactgg cagccgcatg gctaccaagg gttcgggttc
     2281 ttcatccatg gcctgtggag cggttcaccg ccagacaccc gaggcaggag cccggtgcgt
     2341 aaatcgcgca cgccgggatc tgtgcggggg gtatccggta acgggtatcc ctaccgcgac
     2401 atgttggctt taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga
     2461 cggtcgaggt ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt
     2521 gaacggtacg tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg
     2581 gccgggctgg ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga
     2641 gtttggtaac ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg
     2701 tagctctggt ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc
     2761 tactccgggg gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt
     2821 tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat
     2881 ctacggtgtg ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat
     2941 gacgtcatta ccggccgatg cggtgacgga gactccggca agtactttac cactggacca
     3001 ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat
     3061 tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg
     3121 tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa
     3181 aggtgttccg acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg
     3241 tccggctggt tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga
     3301 gaccggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa
     3361 acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga
     3421 ggggctgaaa agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat
     3481 tgcgacctta aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc
     3541 tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac
     3601 ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa
     3661 ggatattcag aatcagaatg agaaattaaa agcggctcag gcatctttaa acgcaatgaa
     3721 tgaatcctta tccagagata aggctgcact taccggggcg atggagagcc ggaaacaaaa
     3781 ggagaaaaaa gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa
     3841 ggggactaaa gactacggcc atgattatca tccagtacct aaaacagaag acataaaggg
     3901 acttggagag ttaaagcgag gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg
     3961 ggcccgatgg tatggcgata aaaaacgtaa aatttatgaa tgggattccc agcacggtga
     4021 gcttgaaggg tatcgtgcca gtgatggcga acacctaggc gcatttgatc ccaaaacggg
     4081 taagcaggtt aaagggccag atccaaaacg gaacattaaa aaatatcttt gagaggtaaa
     4141 tatggggctt aaattacgtt tgacatggtt tgataagaaa actgaagagt ttaaagggga
     4201 agagtattct aaagactttg gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt
     4261 aaaggataat attaataatg gtgagtttga tttgaagaaa gaatgggttc ctttactaca
     4321 gccatttttt aaaaatcaaa taaaacctga tgtggataat tgttatatag cttttgatta
     4381 tcgggacggt aactggtaac aaaaaattat caaggatgat ttgaaatact gacttccggt
     4441 gttcagggat gaatgccgga gtctttaaat acagggaaag tatatgaaaa aggtaaagac
     4501 tatttttctt tttatattga ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta
     4561 tatccgggat gttcagggcg gaacggtggc accatcgtcc tcctctgaac tgacggggat
     4621 cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4681 ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt
     4741 tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct
     4801 tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa
     4861 tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg tgctttcccg
     4921 ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggagtt
     4981 cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac cagtgtgtga
     5041 gctatgagaa agcgccacac ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg
     5101 gcagggtcgg aacaggagag cgcaagaggg agcgacctac cggaaacggt ggggatcttt
     5161 aagtcctgtc gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca
     5221 gatgcaccga aaaagcgtct gtttatgtga actctggcag gagggcggag cctatggaaa
     5281 aacgccaccg gcgcggccct gccgttttgc ctcacatgtt agtcccctgc ttatccacgg
     5341 aatctgtggg taactttgta tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt
     5401 agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg
     5461 cgctgatgtg ggatattgtc ccctgattaa gtctttctat tccctgacga tcaattatca
     5521 ggataggtga accatgtcgg tttttcgctg aacattttac gttaacctat ctctgttgct
     5581 tttttctcct tgcggagaaa tgcaagtcat ccccgctggg gcgtggctga cttcagggga
     5641 aaagaccttg cttacgctgg ctggacgcca ccgacgcacg gtcttttccc ctctttctgg
     5701 ctctgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc
     5761 ctgaccaccc acaaaaatcc acggtgctgg acgcacctga cgaagcagca aagctgctcc
     5821 tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg
     5881 aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc
     5941 tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg
     6001 aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt
     6061 gcagcggggg ctgcgcatca cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt
     6121 ttaatacgga ttcgcttgcc tggcagtggc atatcccgga gctggcccgg cagatttacc
     6181 cgtgggtcag ctggctgtac cgggagtatt acagtctggc cggttttttt ctggttatcg
     6241 gcctgttttt cggtctggct gaactctggg ccggccgaat gcgttttgtg gggctgagcc
     6301 ataaggcgcg gcgggagtat gccgatcgct ataagcgcga taataaccgg actgtgccat
     6361 tttcggtgct ggcctcgctg gattcacccg ggcggatggt cagggcacgg cgacatgtct
     6421 ggaactacgc gtactggctg accggcgctg ccggtctggc ccttctgggg gggctggttg
     6481 tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg
     6541 cagattatta cctgctgcag ccggctaacc tgttcgggat agcctggtgt gccggagggc
     6601 ttgtcgccgg cgtccttctg gcgcggctgc tggcgttcct gctgcttgat ggtcattttg
     6661 cggccgctga cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc
     6721 ggaccggaga aatgacagac gtccgccacc tgcatttcgg ggagccggta ccggttaacg
     6781 cgctggccga tttttccacg gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg
     6841 atgagcaggg ccagccggtg ctggtccctc gtgatacctg gcgcaagacc aatattcaga
     6901 tcctcggcct gcccgggagc ggcaagagcg cgatggccac caacgcgctc attcgctgcg
     6961 tacgcgattt cggggacgct gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc
     7021 acgtcttccg ggcgcactgc ccggacttca ccctgctgga tctgcggccg gggaaaccgg
     7081 cgcagctgaa tctgttccgc gatctcgacc agtacgccct gaagaatctg ctggttgccg
     7141 ggtttaacct gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag
     7201 cggcgaagct gattgcggag cagttcccgc agggggcaaa cattcagcag gtgctggcgg
     7261 cggcgtacgc gttgccggag gccctgaaaa aggacgtgaa ggggctgatc accaagctgg
     7321 agaacgtggc cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg gcggggataa
     7381 tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg
     7441 tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga
     7501 ggtggccgca cgcgagcatc atgctcgatg agattaaata tctgctgtcg aagtacgtgc
     7561 tgaacgcgct ggggacgctg cgcagccggg actgcaacct gctgctcgcg caccagtcgc
     7621 tgggcgactt cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa accacggtac
     7681 tggataacac gccgatccgc tggttctatc gcgcggccag ccaggagtcc gcgcagtggg
     7741 cggcgggcca gaccggggag atccgggtgg acgtggagcg ccgccgggcc agccgggagg
     7801 cggggaacgt ggagcacatc agcggcgaca gcttcatcca gaaggaggcg cggccgctgt
     7861 ttgacgtgaa caccctgcaa cacctgcctg acgggttcgc ggtgatgacc gggctgggcg
     7921 tggcgcggct ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa
     7981 ccttcccggt gctggcgaaa acggacccgc tggcggagta cctgcctccg gccgacggcg
     8041 gcggaccaaa agcggatgat gattttgcgg ggctttactg atgacactgg agcagtacaa
     8101 cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acacggctga ccctgcgctg
     8161 gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg ggattgcggc
     8221 ggtacagccg gtttaccgct tcctggacag tctggtggcg aaagggctgc tggtccgggc
     8281 gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc
     8341 gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc cgtcaagggt
     8401 gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgcctct ccatggaggg
     8461 ccggggggcc accggctggc ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc
     8521 ggacgcgctg gcggagctgg acggcagaac ggtggcattt gaggtagagc gcaccgtcaa
     8581 gagccgcagg cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg
     8641 catcgatgaa atctggtaca tctgccctga ccggccgacg cttgtccggg tacagcgggc
     8701 gattctggcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggtgga
     8761 gctggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacggagt gagggattat
     8821 ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc gctgcattat
     8881 gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt
     8941 tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga
     9001 cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa atccgcaatg
     9061 ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa aggtggagcg
     9121 gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat
     9181 taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct
     9241 gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact
     9301 gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac gccagcaccg
     9361 gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc agagtgtttc
     9421 cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca gctcgttcag
     9481 cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga gggcagtttt
     9541 ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac cagccggacc
     9601 agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg gcctggagtt
     9661 tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta tcttccagca
     9721 tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga agctgttcct
     9781 gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga
     9841 tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg ttgtttttcc
     9901 cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt cttctggtaa
     9961 tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact gtatacccct
    10021 gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc tgaattttac
    10081 ccgttttttc cgttcaggat gtggttttct gacgcgctgc gcttgtcaaa ccggcgacgg
    10141 ccacgcaatg aattgcgccc ccgtcatcgg ttattagagg ctgtctgccc ccggataccc
    10201 ggattctttc tgatgcctga cggtgacgat cacaatctgg tccatttccc ggtcgtggcg
    10261 gtagagcatc acatagccac tgtcgccaaa cccgatcacc agttccttat attccagcgg
    10321 cagaaacggc accgggcggc caatgtctgg caatgttttc agttgctgga tagcccggac
    10381 gatcacctca cctccttttc tggctgccag ccgatttttg gttttgagaa agtcctgaag
    10441 gcgttgtaaa tcgcgctgtg ccagcgcgga aatcgttacc tgtggcatgg cggagcatcc
    10501 tgctcgttct cagttcccca ggtacttatc caggcttccg cttcttccgc agtaaggtgc
    10561 agacccgttt cctgatagtg ctgccatgcg gcctgtccgt cccgcaggta ctggctgcgc
    10621 ttctcttccc ggtcgatgta ttcggtgatt gcctccagca ttagcgcgtg cgctgagcgg
    10681 tgccggtcat cggccagcgt tttcagccgg tccttaagtt cttcatcgag cctgatggat
    10741 gttgctgatg acataaaagc ccctttgtag tcatatgtat tacacatgac tactttaatc
    10801 cgtggcggcg ctatttgcca gaaatacggc ttttgctgcc catttttcag cttcgcttcc
    10861 actaatcagg gctgctacag gctcactcat catccaggac tattgcagcc tgcgggttaa
    10921 aggacctgtt actggcggca accatcatca ttgagctttt gggtggtgac tggtcgcgga
    10981 tcctgtaagc cgtcattttt cagactgaat tcaaaattta taacctgtcg cattgccggg
    11041 tgccctgcgg gatatagcca gtgcctcatt gccgtcagcg tttcccgaaa aaaaaggtga
    11101 cagccggtaa gcgacacgtt gtccactcgt ccatcgtcca gaatatcaaa cgtggcggtc
    11161 acctgacctt cgatgcgttt tgctgcggcc tgtaccgggt aggttggctg cagtgtggtt
    11221 acgggtttta gtggtatcgc atgtaccgga gtgaggaaca tggccgccag cactgtcaga
    11281 gtaaaagctt tcatggtctg aaatccagag gggcgttttt gaatatttgc caggtatttt
    11341 catccggtgc cggcggaatt ttggcgtggg taacggctga tattgctgct ttacagagtt
    11401 tcggatctcc tccttcagca atggcgctgt tcagagtgcc atcacgttgt atggatatcc
    11461 gcacggtgca catttttccc gcataggcct ctggttcaaa catctgcatt tcaacagcgg
    11521 tcttgatggc cgcagcgtag ctactgattt tagatgactt atccagagta taaattttcg
    11581 gcggctgggt ggtggaatgc ttacaggccg tcaggagtaa ggggctgcac aatgccagca
    11641 ggataaaggg tattaaacgc atcgggttga tgacctgtca gtaaaaaatg ttacatgtat
    11701 atttgtcact ctttacggcc acgttcattt acataaacac ggcttatcgt atcgttttta
    11761 caccaaacgg cccgccatgc agttgcgtgc ataggcatgc agcgggttac aatttaccgg
    11821 gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt ttatcccgta gccgccggaa
    11881 acgccctcag accgtctgag cggtgcgggc aatgcgacgt tatggtaaat accccttacc
    11941 ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc c
//
