LOCUS       NZ_CP129813            11970 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020N06-155 plasmid p2020N06-155-11k,
            complete sequence.
ACCESSION   NZ_CP129813
VERSION     NZ_CP129813.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281318
            Assembly: GCF_030490125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 36x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,811
            CDSs (total)                      :: 5,687
            Genes (coding)                    :: 5,551
            CDSs (with protein)               :: 5,551
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 49 of 136
            Pseudo Genes (incomplete)         :: 86 of 136
            Pseudo Genes (internal stop)      :: 11 of 136
            Pseudo Genes (multiple problems)  :: 10 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129813.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020N06-155"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020N06-155-11k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            478..2442
                     /locus_tag="QYQ89_RS28990"
                     /old_locus_tag="QYQ89_28990"
     CDS             478..2442
                     /locus_tag="QYQ89_RS28990"
                     /old_locus_tag="QYQ89_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2442..3173
                     /gene="mobC"
                     /locus_tag="QYQ89_RS28995"
                     /old_locus_tag="QYQ89_28995"
     CDS             2442..3173
                     /gene="mobC"
                     /locus_tag="QYQ89_RS28995"
                     /old_locus_tag="QYQ89_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3180..3710
                     /locus_tag="QYQ89_RS29000"
                     /old_locus_tag="QYQ89_29000"
     CDS             3180..3710
                     /locus_tag="QYQ89_RS29000"
                     /old_locus_tag="QYQ89_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3737..3916)
                     /locus_tag="QYQ89_RS29005"
                     /old_locus_tag="QYQ89_29005"
     CDS             complement(3737..3916)
                     /locus_tag="QYQ89_RS29005"
                     /old_locus_tag="QYQ89_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3942..4370)
                     /locus_tag="QYQ89_RS29010"
                     /old_locus_tag="QYQ89_29010"
     CDS             complement(3942..4370)
                     /locus_tag="QYQ89_RS29010"
                     /old_locus_tag="QYQ89_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4534..4848)
                     /locus_tag="QYQ89_RS29015"
                     /old_locus_tag="QYQ89_29015"
     CDS             complement(4534..4848)
                     /locus_tag="QYQ89_RS29015"
                     /old_locus_tag="QYQ89_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4836..5114)
                     /locus_tag="QYQ89_RS29020"
                     /old_locus_tag="QYQ89_29020"
     CDS             complement(4836..5114)
                     /locus_tag="QYQ89_RS29020"
                     /old_locus_tag="QYQ89_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5289..5654)
                     /locus_tag="QYQ89_RS29025"
                     /old_locus_tag="QYQ89_29025"
     CDS             complement(5289..5654)
                     /locus_tag="QYQ89_RS29025"
                     /old_locus_tag="QYQ89_29025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5651..6022)
                     /gene="tolA"
                     /locus_tag="QYQ89_RS29030"
                     /old_locus_tag="QYQ89_29030"
     CDS             complement(5651..6022)
                     /gene="tolA"
                     /locus_tag="QYQ89_RS29030"
                     /old_locus_tag="QYQ89_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6296..6541)
                     /locus_tag="QYQ89_RS29035"
                     /old_locus_tag="QYQ89_29035"
     CDS             complement(6296..6541)
                     /locus_tag="QYQ89_RS29035"
                     /old_locus_tag="QYQ89_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7186..8673
                     /gene="ltrA"
                     /locus_tag="QYQ89_RS29040"
                     /old_locus_tag="QYQ89_29040"
     CDS             7186..8673
                     /gene="ltrA"
                     /locus_tag="QYQ89_RS29040"
                     /old_locus_tag="QYQ89_29040"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            8778..10463
                     /locus_tag="QYQ89_RS29045"
                     /old_locus_tag="QYQ89_29045"
     CDS             8778..10463
                     /locus_tag="QYQ89_RS29045"
                     /old_locus_tag="QYQ89_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10473..10730
                     /locus_tag="QYQ89_RS29050"
                     /old_locus_tag="QYQ89_29050"
     CDS             10473..10730
                     /locus_tag="QYQ89_RS29050"
                     /old_locus_tag="QYQ89_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            10815..10964
                     /locus_tag="QYQ89_RS29055"
                     /old_locus_tag="QYQ89_29055"
     CDS             10815..10964
                     /locus_tag="QYQ89_RS29055"
                     /old_locus_tag="QYQ89_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             3778..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11068..11646)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2443..3170
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 aaaagacctt gcttacgctg gctggacgcc accgacgcac ggtcttttcc cctctttctg
       61 gctctgtccg gctggacgcc ggccacgcca gaaattcacc cggcccccgt caggctcacg
      121 cctgaccacc cacaaaaatc cacggtgctg gacgcacctg acgaagcagc aaagctgctc
      181 ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc cggaacaccg
      241 gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc
      301 ctccggggct gcgccttttc cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct
      361 gaacgcaatc accacggacg tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg
      421 tgcagcgggg gctgcgcatc acgccggtaa acccgtcgtc tgaaggagag agattgcatg
      481 tttaatacgg attcgcttgc ctggcagtgg catatcccgg agctggcccg gcagatttac
      541 ccgtgggtca gctggctgta ccgggagtat tacagtctgg ccggtttttt tctggttatc
      601 ggcctgtttt tcggtctggc tgaactctgg gccggccgaa tgcgttttgt ggggctgagc
      661 cataaggcgc ggcgggagta tgccgatcgc tataagcgcg ataataaccg gactgtgcca
      721 ttttcggtgc tggcctcgct ggattcaccc gggcggatgg tcagggcacg gcgacatgtc
      781 tggaactacg cgtactggct gaccggcgct gccggtctgg cccttctggg ggggctggtt
      841 gtcgccgtgt ttatcgagag ctggggagaa accgtcctgc gcccggacct ctatgcgaac
      901 gcagattatt acctgctgca gccggctaac ctgttcggga tagcctggtg tgccggaggg
      961 cttgtcgccg gcgtccttct ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt
     1021 gcggccgctg acgaggcggt gaacgcgaaa ctgaatcagg agagccggcg cagcagccag
     1081 cggaccggag aaatgacaga cgtccgccac ctgcatttcg gggagccggt accggttaac
     1141 gcgctggccg atttttccac ggagcaggcc cggaaacagc aggcggtgtt tctgggtaag
     1201 gatgagcagg gccagccggt gctggtccct cgtgatacct ggcgcaagac caatattcag
     1261 atcctcggcc tgcccgggag cggcaagagc gcgatggcca ccaacgcgct cattcgctgc
     1321 gtacgcgatt tcggggacgc tgtggtgtac ttcgacccca aaggggacgc gtgggcgccg
     1381 cacgtcttcc gggcgcactg cccggacttc accctgctgg atctgcggcc ggggaaaccg
     1441 gcgcagctga atctgttccg cgatctcgac cagtacgccc tgaagaatct gctggttgcc
     1501 gggtttaacc tgagtgagac cggcgacgtg gcggaccact accgcatcag cgagcagaaa
     1561 gcggcgaagc tgattgcgga gcagttcccg cagggggcaa acattcagca ggtgctggcg
     1621 gcggcgtacg cgttgccgga ggccctgaaa aaggacgtga aggggctgat caccaagctg
     1681 gagaacgtgg ccgacctgag cgtcctgcag acggacagcg gtatcgatgt ggcggggata
     1741 atcaacggcg gcggctgcct gtacgtcatc ggctcgatgg acgatgaggc ggtgatccgg
     1801 gtgcagaaga tgctgtttgc ccgctgcgcg caaatcatca ttgcgcggga tgagttccgg
     1861 aggtggccgc acgcgagcat catgctcgat gagattaaat atctgctgtc gaagtacgtg
     1921 ctgaacgcgc tggggacgct gcgcagccgg gactgcaacc tgctgctcgc gcaccagtcg
     1981 ctgggcgact tcggtcagtg cgggcaggat ctgcccgctg attttgtgaa aaccacggta
     2041 ctggataaca cgccgatccg ctggttctat cgcgcggcca gccaggagtc cgcgcagtgg
     2101 gcggcgggcc agaccgggga gatccgggtg gacgtggagc gccgccgggc cagccgggag
     2161 gcggggaacg tggagcacat cagcggcgac agcttcatcc agaaggaggc gcggccgctg
     2221 tttgacgtga acaccctgca acacctgcct gacgggttcg cggtgatgac cgggctgggc
     2281 gtggcgcggc tggcgttcag cagcccgctg cgggttgagc gccgggaaat ttcgctgaaa
     2341 accttcccgg tgctggcgaa aacggacccg ctggcggagt acctgcctcc ggccgacggc
     2401 ggcggaccaa aagcggatga tgattttgcg gggctttact gatgacactg gagcagtaca
     2461 acgtcagcca cgcgaagcgg caggcgcgga acgcggagaa aacacggctg accctgcgct
     2521 ggctgcggga agagctgtgt tccacggcgg agctggttgc gcgacggctg gggattgcgg
     2581 cggtacagcc ggtttaccgc ttcctggaca gtctggtggc gaaagggctg ctggtccggg
     2641 cgaaataccc ggttgacggc cgtcaggtca gcgtgtgggg gctgacgccg cacggggtgg
     2701 cgttctcgtt cgacgaggac gagccgctga cggacgttat tccgtttcag ccgtcaaggg
     2761 tgtcggcggc gcagctgccg caccggctgg ccgtgcagtc cctgcgcctc tccatggagg
     2821 gccggggggc caccggctgg cggtacctgc accgtatggc cctgaaaggg atgaaagtgc
     2881 cggacgcgct ggcggagctg gacggcagaa cggtggcatt tgaggtagag cgcaccgtca
     2941 agagccgcag gcggtatcag gaagtggtgt ccgggtatct gttcaaccgc cgggcgaacg
     3001 gcatcgatga aatctggtac atctgccctg accggccgac gcttgtccgg gtacagcggg
     3061 cgattctggc ggtggatgaa atcgtgaatc cacagaccgg cgaggcccgc aaaacggtgg
     3121 agctggaccg ggagaggctg ttcgcgtgct ttaaatttat gacaacggag tgagggatta
     3181 tggcgctgta catggtattt attgaggacg tggaagacaa agacggcgag cgctgcatta
     3241 tgccaaccag cgagcatctg gaagacatgg tggtgctcgc ctggcagacc ggggcgtatg
     3301 ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat gaaatccatc gggatgtatg
     3361 acgagcggct caacagcgac gaaaaagcca cgtacttttt caccggtgaa aatccgcaat
     3421 gggaacggga caaacccaca ggaatgaagc tgtgtgatta cctctatcga aaggtggagc
     3481 ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg gaagtgctca ccggagcaga
     3541 ttaacgcgat gattgaggtg gtcagcgagc ggcacgggga aagcgcggtg aacgagaagc
     3601 tgaaagcgga gctgatgcgg gaatataaca gccgctattt taacgtgccg gtgcctgaac
     3661 tgccggagga caaacccgcc cgccggtggt ggccgttcgg caaaaaataa cgccagcacc
     3721 ggcatggccg gtgctgttat tcttccccga gcttatcccg catcacctgc cagagtgttt
     3781 ccgcatcctc gtgaagccgt tcgcacatgt cggccggggt atccatgtcc agctcgttca
     3841 gcttttccag caacagcagc gactgatcct taatgaattt agccatattg agggcagttt
     3901 tttcctgctt cgtcatgttc tccattccct tcggtaagtt ctcagctaaa ccagccggac
     3961 cagaagcgtt ttttcttctg tgtcagttct gttgtcaatc tgtccacttc ggcctggagt
     4021 ttccggcgct catcatcgtg ctggcggcgt tcttcttccc gggatgtttt atcttccagc
     4081 atcagggtga cagcctgccg gagatcggcc agttcctttt gcattgccag aagctgttcc
     4141 tgtgggaaat ttttttgtgt attttcacgg tgtgaaatgc cgggaatttg tttctcaggg
     4201 atattcacat ttccgtatac acggatcagt tctgacacat ccacgacagg gttgtttttc
     4261 ccgtcacgtg acacggtaac ctttccctgt tttacatgat tgtatagcgt tcttctggta
     4321 atgcccgcgg cttgtgctgc ctgtgaaagg ttgagcaatg ttttcgccac tgtatacccc
     4381 tgattttatc gggtgtgaaa ggtgtgtaat ttctcacggt aaatgtatcg ctgaatttta
     4441 cccgtttttc cgttcaggat gtggttttct gacgcgctgc gcttgtcaaa ccggcgacgg
     4501 ccacgcaatg aattgcgccc ccgtcatcgg ttattagagg ctgtctgccc ccggataccc
     4561 ggattctttc tgatgcctga cggtgacgat cacaatctgg tccatttccc ggtcgtggcg
     4621 gtagagcatc acatagccac tgtcgccaaa cccgatcacc agttccttat attccagcgg
     4681 cagaaacggc accgggcggc caatgtctgg caatgttttc agttgctgga tagcccggac
     4741 gatcacctca cctccttttc tggctgccag ccgatttttg gttttgagaa agtcctgaag
     4801 gcgttgtaaa tcgcgctgtg ccagcgcgga aatcgttacc tgtggcatgg cggagcatcc
     4861 tgctcgttct cagttcccca ggtacttatc caggcttccg cttcttccgc agtaaggtgc
     4921 agacccgttt cctgatagtg ctgccatgcg gcctgtccgt cccgcaggta ctggctgcgc
     4981 ttctcttccc ggtcgatgta ttcggtgatt gcctccagca ttagcgcgtg cgctgagcgg
     5041 tgccggtcat cggccagcgt tttcagccgg tccttaagtt cttcatcgag cctgatggat
     5101 gttgctgatg acataaaagc ccctttgtag tcatatgtat tacacatgac tactttaatc
     5161 cgtggcggcg ctatttgcca gaaatacggc ttttgctgcc catttttcag cttcgcttcc
     5221 actaatcagg gctgctacag gctcactcat catccaggac tattgcagcc tgcgggttaa
     5281 aggacctgtt actggcggca accatcatca ttgagctttt gggtggtgac tggtcgcgga
     5341 tcctgtaagc cgtcattttt cagactgaat tcaaaattta taacctgtcg cattgccggg
     5401 tgccctgcgg gatatagcca gtgcctcatt gccgtcagcg tttcccgaaa aaaaaggtga
     5461 cagccggtaa gcgacacgtt gtccactcgt ccatcgtcca gaatatcaaa cgtggcggtc
     5521 acctgacctt cgatgcgttt tgctgcggcc tgtaccgggt aggttggctg cagtgtggtt
     5581 acgggtttta gtggtatcgc atgtaccgga gtgaggaaca tggccgccag cactgtcaga
     5641 gtaaaagctt tcatggtctg aaatccagag gggcgttttt gaatatttgc caggtatttt
     5701 catccggtgc cggcggaatt ttggcgtggg taacggctga tattgctgct ttacagagtt
     5761 tcggatctcc tccttcagca atggcgctgt tcagagtgcc atcacgttgt atggatatcc
     5821 gcacggtgca catttttccc gcataggcct ctggttcaaa catctgcatt tcaacagcgg
     5881 tcttgatggc cgcagcgtag ctactgattt tagatgactt atccagagta taaattttcg
     5941 gcggctgggt ggtggaatgc ttacaggccg tcaggagtaa ggggctgcac aatgccagca
     6001 ggataaaggg tattaaacgc atcgggttga tgacctgtca gtaaaaaatg ttacatgtat
     6061 atttgtcact ctttacggcc acgttcattt acataaacac ggcttatcgt atcgttttta
     6121 caccaaacgg cccgccatgc agttgcgtgc ataggcatgc agcgggttac aatttaccgg
     6181 gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt ttatcccgta gccgccggaa
     6241 acgccctcag accgtctgag cggtgcgggc aatgcgacgt tatggtaaat accccttacc
     6301 ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc ctcgtatttc tcccgacgca
     6361 tttcctgcgc ccgcctggct tcacgttcgt cctgaatggc acgaagcacc ctttcccgca
     6421 cctgttgttt taccgccttt ggcagcgttt tgttcccttt caggcccatg gccagtgcct
     6481 gagcgacaaa ggcgtccgcc aggcgctttt cttcagcggg agtcagtttc aggtgcgtca
     6541 tagtggcctc cgggcgtctc aggtttcccg aacagtttaa ccgcaggtcc tctcctcatg
     6601 ccagttagct ttcagatgtg tctataccat ctgaaatgta agtgtgatga atgtcttaaa
     6661 taataaaatt tgttgccatg agaaattttc gggaaaagac gcgatctgct tacaggtggc
     6721 tccgggcgct tttttgtgcc tgaagatgag aactaatctc atatattgac acctgaaaac
     6781 gggaggagta aggtaataat tatactgtgt atatgtacag tgtgcgccgg gagaccggtt
     6841 agagatcaag gggtgaaagt ccccgaccat tgaaggacca gcaatccaca aggtccccga
     6901 gtcatgcgtt gcataccgcg aggtatgggg cgaagcgttg acaggggtgt tgacaggcca
     6961 gccattgagc cacgaaatgt atattaaatt accgggtgcc gacgttgtac tgttaacgga
     7021 aggcaacatc atagggtgcg atactgcgag tgccacatgg acccggcggg gtctgagacc
     7081 ctggcatgtc aatacgatct ctacgcggga accgggagat ctcccctctg accatctgcc
     7141 agtgtcggag atggcccgca ccgggaagac gaggagtcat agccggtgat gtacggagag
     7201 gagaagtcgg actcgctcat agtagcggcg aagcaggcga acaacccgaa aggagcggag
     7261 tcagtggagc gaaggagcgg ggccaagggg aacgcggaac agccacacat gcgccggaca
     7321 cagagccggg aaagcatgtc acagaggctg tcacgcgtgc gggaagctgc gaagcagcgg
     7381 aagaaagaac ggtttacagc attgttccac ctgctgacag tcgaagcact ggaagccgca
     7441 ttcctctccc tgagcaggaa agcggccgcc ggagtggatg gcatcaggtg gatggactac
     7501 gccggaaaca tgaagaacaa cataacagat ctgcaccgga ggctacatca gggcagctac
     7561 agggcgcagc ccggcaggcg tcactacatc ccaaaagcgg atggaaaaca acgcccgctc
     7621 ggcatcgcct cgctggagga caagatcgtc cagtatgcgc tggtgaaaat cctgaacgca
     7681 gtctatgaaa acgactttat ggggttctca tacgggttca gacccgggcg aagccagcac
     7741 gatgcactgg acgcactggc cacagggctg gtacgcacta acgtaaactg ggtactggat
     7801 gccgacatca gtcagttctt cgacagggtg agccacgaat ggctgatcag gttcacagag
     7861 catcggatcg gcgaccggag ggtaatcagg ctcatacgta agtggctcac agccgggacg
     7921 tcggaggagg gtcaatggcg agcaacggag gaaggcaccc cacagggtgc ggtcatctca
     7981 ccgctgctgg caaacatata cctccactac gtcttcgatc tgtgggcgca tcagtggcga
     8041 cgtcgctatg ccacaggcaa tgtggtaatg gtcagatacg ccgatgacat cgtcatcggg
     8101 ttcgacaaac gatacgatgc ccggcgcttc cgtatagcca tgcagcgcag actgagggag
     8161 ttcggactca cggttcaccc ggagaaaacc cgtctgatgg agttcggccg cttcgctgcc
     8221 gaaaaccgtg ccatcagggg aaaaggcaaa ccagaaacgt tcaacttcct cgggttcacg
     8281 cacatcagcg ggaaagatcg caacggcagg ttcatgctga tacgaaagac ccgccgggat
     8341 cggatgacgg caactctgaa agccatcaaa gacggtctgc gaaggcgctg gcattactca
     8401 atccccgaac agggaaaatg gctcaggaga gtggttcagg gatacctgaa ctatcactcg
     8461 gtaccgggca acttccccac catgcagaag ttcaggacac acgtaacaaa cctctggcgc
     8521 cgggcgctca ggcgcaggag ccagaaggat gatacgacct ggacgaaagc aaacaaactg
     8581 gcagccgcat ggctaccaag ggttcgggtt cttcatccat ggcctgtgga gcggttcacc
     8641 gccagacacc cgaggcagga gcccggtgcg taaatcgcgc acgccgggat ctgtgcgggg
     8701 ggtatccggt aacgggtatc cctaccgcga catgttggct ttaatttatt gttttaaatg
     8761 tcaaagagga aaacggtatg agtggtggag acggtcgagg tccgggtaat tcaggtctgg
     8821 gacataatgg tggtcaggcc agtgggaatg tgaacggtac gtctggtaaa ggtggccctt
     8881 catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg catacgccga
     8941 atggagatat tcataactat aatccggggg agtttggtaa cggcgggagt aaacccggtg
     9001 gtaatggtgg taacagcggc aatcatagcg gtagctctgg tggcggacag tcttcggcca
     9061 ctgcgatggc cttcggtctg cctgccctgg ctactccggg ggctgaagga ctggctttat
     9121 cagtttccgg cgatgcgttg tcggccgccg ttgctgatgt gctggctgcc ctgaaggggc
     9181 cgtttaagtt tggtctgtgg gggattgcga tctacggtgt gctgccttct gagattgcaa
     9241 aagatgatcc gaaaatgatg tcaaaaatta tgacgtcatt accggccgat gcggtgacgg
     9301 agactccggc aagtacttta ccactggacc aggcgacggt tcgtgtcaga caacgggttg
     9361 tggatgtggt gaaggatgag cggcagcata ttgcggttgt cgcaggtcgg ccaatgagtg
     9421 tccctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg
     9481 gtctcccgtc tctgcaggtg agcgtaccga aaggtgttcc gacagcgaaa gccccgccaa
     9541 aaggcattgt tgctgaaaaa ggtgattcac gtccggctgg ttttacagcc ggtggtaact
     9601 cccgtgaggc cgttattcgt ttcccgaaag agaccggaca gaagccggtt tatgtgtcgg
     9661 tgacagatgt tcttaccccg gcacaggtaa aacagcgtca ggaggaagaa aagcgtcgcc
     9721 agcaggcatg ggacgccgct catccggaag aggggctgaa aagagactat gataaagcga
     9781 aagctgagct ggatgccgaa gataaaaata ttgcgacctt aaacagccgc attgcatcga
     9841 cagagaaggc gctccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag
     9901 aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg
     9961 ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat gagaaattaa
    10021 aagcggctca ggcatcttta aacgcaatga atgaatcctt atccagagat aaggctgcac
    10081 ttaccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata
    10141 agttaaatga ggaaaagaac aagcctcgca aggggactaa agactacggc catgattatc
    10201 atccagtacc taaaacagaa gacataaagg gacttggaga gttaaagcga ggtgatccta
    10261 aaacacctaa acaaggtggt ggtggtaagc gggcccgatg gtatggcgat aaaaaacgta
    10321 aaatttatga atgggattcc cagcacggtg agcttgaagg gtatcgtgcc agtgatggcg
    10381 aacacctagg cgcatttgat cccaaaacgg gtaagcaggt taaagggcca gatccaaaac
    10441 ggaacattaa aaaatatctt tgagaggtaa atatggggct taaattacgt ttgacatggt
    10501 ttgataagaa aactgaagag tttaaagggg aagagtattc taaagacttt ggtgacgatg
    10561 gttctgtcat tgaaagcttg ggaatgcctt taaaggataa tattaataat ggtgagtttg
    10621 atttgaagaa agaatgggtt cctttactac agccattttt taaaaatcaa ataaaacctg
    10681 atgtggataa ttgttatata gcttttgatt atcgggacgg taactggtaa caaaaaatta
    10741 tcaaggatga tttgaaatac tgacttccgg tgttcaggga tgaatgccgg agtctttaaa
    10801 tacagggaaa gtatatgaaa aaggtaaaga ctatttttct ttttatattg attgttgctg
    10861 gctttcttct ggccgcatgt caggcaaatt atatccggga tgttcagggc ggaacggtgg
    10921 caccatcgtc ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc
    10981 ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg
    11041 gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt ggtaactggc
    11101 tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt ggcgcacact
    11161 tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aactcccagt taccaatggc
    11221 tgctgccagt ggcgttttgc gtgctttccc gggttggact caagtgaaca gttaccggat
    11281 aaggcgcagc agtcgggctg aacggggagt tcttgcttac agcccagctt ggagcgaacg
    11341 acctacaccg agccgagata ccagtgtgtg agctatgaga aagcgccaca cttcccgcaa
    11401 gggagaacgg cggacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcaagagg
    11461 gagcgaccta ccggaaacgg tggggatctt taagtcctgt cgggtttcgc ccgtactgtc
    11521 agattcatgg ttgagcctca cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg
    11581 aactctggca ggagggcgga gcctatggaa aaacgccacc ggcgcggccc tgccgttttg
    11641 cctcacatgt tagtcccctg cttatccacg gaatctgtgg gtaactttgt atgtgtccgc
    11701 agcgcccgcc gcagtctcac gcccggagcg tagcgaccga gtgagcgagg aggcgcaatt
    11761 tccccggcac ctgcaagacg gtcaccggac gcgctgatgt gggatattgt cccctgatta
    11821 agtctttcta ttccctgacg atcaattatc aggataggtg aaccatgtcg gtttttcgct
    11881 gaacatttta cgttaaccta tctctgttgc ttttttctcc ttgcggagaa atgcaagtca
    11941 tccccgctgg ggcgtggctg acttcagggg
//
