LOCUS       NZ_CP129808             5630 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020C08-162 plasmid p2020C08-162-5k,
            complete sequence.
ACCESSION   NZ_CP129808
VERSION     NZ_CP129808.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281338
            Assembly: GCF_030490105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 31x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:07:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,981
            CDSs (total)                      :: 5,857
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 59 of 118
            Pseudo Genes (incomplete)         :: 58 of 118
            Pseudo Genes (internal stop)      :: 12 of 118
            Pseudo Genes (multiple problems)  :: 10 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129808.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020C08-162"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020C08-162-5k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            144..674
                     /locus_tag="QYQ37_RS29880"
                     /old_locus_tag="QYQ37_29880"
     CDS             144..674
                     /locus_tag="QYQ37_RS29880"
                     /old_locus_tag="QYQ37_29880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            681..914
                     /locus_tag="QYQ37_RS29885"
                     /old_locus_tag="QYQ37_29885"
     CDS             681..914
                     /locus_tag="QYQ37_RS29885"
                     /old_locus_tag="QYQ37_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1395..2447
                     /locus_tag="QYQ37_RS29890"
                     /old_locus_tag="QYQ37_29890"
     CDS             1395..2447
                     /locus_tag="QYQ37_RS29890"
                     /old_locus_tag="QYQ37_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001053268.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2449..3315
                     /locus_tag="QYQ37_RS29895"
                     /old_locus_tag="QYQ37_29895"
     CDS             2449..3315
                     /locus_tag="QYQ37_RS29895"
                     /old_locus_tag="QYQ37_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4517..4708)
                     /locus_tag="QYQ37_RS29900"
                     /old_locus_tag="QYQ37_29900"
     CDS             complement(4517..4708)
                     /locus_tag="QYQ37_RS29900"
                     /old_locus_tag="QYQ37_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4773..5096
                     /locus_tag="QYQ37_RS29905"
                     /old_locus_tag="QYQ37_29905"
     CDS             4773..5096
                     /locus_tag="QYQ37_RS29905"
                     /old_locus_tag="QYQ37_29905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          4342..4423
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          3699..3885
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.471"
                     /coverage="100.0"
                     /evalue="7.74e-92"
     Typing          3..1018
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5087..5628
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="4.76e-115"
ORIGIN
        1 ccatgccgga gagataaaag gccgtcagga tgtgattcag gcgctcacag aggcggggct
       61 tgaagtggtc agggtgacgc gaagcagtat cagcattgca gacccgaacg gcgggaggaa
      121 tatcagactg aaaggagcgt tttatgagca atcttttaca gacgggcgcg gagttcgaga
      181 aaaagctgaa agagagagcc gaatctacag agaaaatgct gaacgacgag ttcaggaagc
      241 tcgaagaatc tgtaaacagg gaactgacct caaacgaatc tttaatcagg aacgctatca
      301 acgaccatac aacagccctg aaagaactgc tggagaggta tcagaaaacg accgtcgata
      361 cgatggacgc gcactggaaa accgtactga aaatgagcgt gaaacgctgg ctgtggctga
      421 ttatcgtctc agtactgatg tttgccacga ccgggagtct gctgtggtac cagggcatga
      481 agataaacgc gaatatgaat attctccgcg agcagaaaga gagtctggag aagctgaacg
      541 cgaagacatg gggcgtgcgt tatcacgagg acagcaacgg acgttttctg gtgttgccga
      601 aggggatgaa agccgaaacg aactggacga aggacaacgg caaactgaac gcagtgagac
      661 tggtgcagga gtgacagagc atgacggagc tggaaagaca tttgctgaac gcattagagc
      721 aactgcaaca ggactatatg cagcggctga acgaatggga gaacgccttc gtggaattgc
      781 agaaaatgtt ttcgcttacg caacgggaca acgcgatgct gaacgggcga gtcatgcagt
      841 tgagtcagca ggtgcagcac ttgagcgagc ggacagaacg cttgagccag ttatacagcg
      901 agaacaggag ataagggatg aacggctgat gcaggaacgc gaacatgagt tatctctgga
      961 gcgggagcgt cagccggaaa tccaggagcg gacactggat ggcccttcgc tgggatggtg
     1021 aaaccatgaa aaatgacgtg ttcagtggat taaggcgggc taatgtggcc tgtagcatct
     1081 ggttgcatag gtattcatgc ggttaaaatt tatcaggtgc gatcgcggca gtttttcggg
     1141 tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc accgaacgcg ttttggcggt
     1201 gcgtacaatg cgacgttatg gtaaatggag cggattttca ggaaggagtg atactaacaa
     1261 ctcgaacact gtgcaaataa tgattttttt aaaatatcgg taacgataat aaattatttc
     1321 gttttacgcc ggtaataaat ataatgtagt ttcagtatat aaaaaaatat atgaggtgtc
     1381 ttgtgggagc tttcatgaat cttcaacaac gtataaataa actaccacaa ttatcatcca
     1441 gtttttcatt tggaaaagat attgataata ttcattcatt tatctttaac gaaacttcta
     1501 aagataaaat tgaagattta ctaagaaaat gggtgtcagg caatcagccc tgtgtatttg
     1561 gtaaattggc aagcaagaaa attaaagggc ttgatttcca tttatctatt gtaaatagcc
     1621 cccaactata taatgatgat ggtcatttgt ttgatttttt gcgtaatgaa agagttcgtt
     1681 ttaaagaacg cgcaagaaga ggagaggtca gtgcgcatct catttatttt attcatccac
     1741 aacttgcctt tgctcgtcca tcagaagagt tagtggacat acaaaaatat atttgttcgc
     1801 tgcatatgcc agagtgctat ccaataaaag aggatgttat ttatactgaa agcgtaccat
     1861 ttcaggataa agatgggcta aaaatatata aggctggtgt taatgttttt tactcttctg
     1921 ctcatagaac aagaaatcat gatagaagaa taccaggggg aatacttatt tctgtaaatg
     1981 ccccggggca ttttatgagg ctggcgatag aaaaaggttt ctataaagat caagaacaag
     2041 cgttagcgga tattcgtaac atgactattc agtctgttgg taagggtggg tattcacatc
     2101 cggaagggat ttctacaaca tggcattcag aatctaattt ggatcgtttt ggatgcccag
     2161 tgcacaccgg taatagttct tattacagcg gtttttatca tactgatgta ttaattccag
     2221 gagaattaac taaggatgaa agattgctcc atgaaataga aaatagtgat ccaatgatct
     2281 tcaactggaa tgtattgttt tatgtttcat tagaagagtt tcctatagat gatccttatt
     2341 atggagagtt tattggtatt cctgtagatg atgcatctat gttttttaac tcctttcaac
     2401 caaggaaatt tgaaaacaaa cccctttatg agaaagagga tgattaatat gattaaagga
     2461 atgctttttg cttttttagc agcgttctca tggggggcag ctatcgtaat gagcaaaaaa
     2521 ggagttgaag aactagatgc cggaggggtg tttttctggc agatttgtag tgcagtaata
     2581 ctatcttgga tagttctttt tataaaaaga aaaaaactcc cattaaactc aaaagcgata
     2641 cttgcttatg cgacaggcgt ttttgaacca ttcttagcgt atacttttac actctacgga
     2701 ctttatttta tttcagcagg aagcgcatca attatttttt cacttgaatc agtgtttata
     2761 ttggtgttgt ctgttcttat ttgctcagtt aaaattaatt ccccgcaata ttttatattg
     2821 ttccttatag gtgcaattgt tggcagtatt ttggtagcct tgccagacat cagtaatgga
     2881 tcgaataata ttgttggata ttgtcttgtt gttgccggtg tgttatcggc agcattttat
     2941 gttgttatat cgtctcgcct tattaaatcg tttgagccta tcacattatt gagcggacag
     3001 ttgacattct cccttatact gtcatcgctc tttattttta tatctaaatc atcaatttat
     3061 cttccgttgg aatccgtgat tattgttttt tcatctggaa tattacaata ttttttagct
     3121 ttttgttttt acttgaactc cttgcgttgg ctcccagttc atttggcagg gataatgttg
     3181 tactttattc cgctagttgc attatttttg tcatggttgt ttttggatga acaattcaca
     3241 ttcttacagg gggtaggatt tattgtaact gtgctaagtg tatattttct taacagaaaa
     3301 tactcagagg agtaacattc tgttcaggat tgccccccgg cctccatcga taccgtgcca
     3361 aatgcacgta aaaaacggga gcgtttctgg tgtcggaatg taaccaaggg ggtcagaggc
     3421 gcgctaaaac gctctcaggg gagtcaaata gagacgtttg taacaaaaag atcaaaggat
     3481 cctcttgaga tccttttttt ctgcgcgtaa tctgctgcct gtaaacgaaa aaaccgcctt
     3541 gcagggcggt ttgatcgaag gttaagtcag cttgcagact gcttaaccag gtaactgtct
     3601 tatgcgagac gagtcaccaa aattgttctt ctagtgtagc cgcactccgg cagccacttc
     3661 aagtactctc taaatatctc gcacatgact tttaccaatg gctgctgcca gtggcgcttt
     3721 gtggtgtctt ttccggttgg acccaagaga acagttaccg gagaaggcgc agcagtcggg
     3781 ctgaacgggg ggttcctgca tacagcccag cccggagcga actgcctaag tcgccccgag
     3841 ataccagaag acgtgagcta tgagaaagcg ccacgtgtcc cgtaagggag aaaggcggaa
     3901 aaggtatccg gtaatcgata tggcgggaac gtgggggcgc aggagggagc gattcaccgg
     3961 aaacggtgat gatctttaag ccacgtcggg tttcgccacc gctggcggat ttcatggttg
     4021 agccgcaagg ctcccacaga tgcaccgaaa aagtgtctgt tttgtgaagt ccgtcagggg
     4081 ggcggagcct atggaaaaag cgcctccggc gcggcctttc ccggggtttc gttttttgcg
     4141 cacatgttct ttcctggttt atccccgggt tctgtggata acagggttag cgtttttgag
     4201 aaaggagagt ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     4261 gcggaagagc gcctgaactg gcattttctc cttacgcatc tgtgcggcat ttcacaccgg
     4321 acatggcgca cttttcatac aatccgcact gatgccgaat ggttaagcca gtatacactc
     4381 cgctatcgct acgtgactgg gtcagggctt cgccccgaca ccccaaaggt caacggcctg
     4441 acgcgccctg gcgggcttgt ctgctcccgg catccgctca cagacaagct gtgaccgtct
     4501 ccgggagctg catgtgtcag cggttttcac cgtcatcccc gaagcgtgcg aggcaactgc
     4561 ggtaaagctc atcggcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     4621 ccagttcgtt gagcttctcc agcagcgtta atgtctggcc tctgatgaag cgggccatgt
     4681 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaagggggg gttctgttca
     4741 tgggggtaat gataccgatg aaacgcgaga ggatgctcac gatacgggtt actgatgatg
     4801 aacatgcccg gttactggaa cgctgtgagg gtaaacaact ggcggtatgg atgcggcggg
     4861 tctgcctggg tgagccggtt gcccgttccg ggaagctgcc gacactggca ccgccgttac
     4921 tgcgtcagct ggccgccatc gggaataacc ttaaccagac agcgaggaag gtgaacagcg
     4981 ggcagtggtc ttccggtgac cgggttcagg tggtggccgc actgatggcc atcggggatg
     5041 agctgcgccg gctgcgtctg gcggtcaggg aacagggggc gcgggatgat agttaagttt
     5101 catgccaggg gaaaaggtgg tggcagtggt ccggttgatt acctgctggg aagggagcgt
     5161 aaccgggaag gggcaacggt gcttcagggg aatccggaag aggtccggga actcatcgat
     5221 gccacgccat ttgcgaagaa atacacgtca ggtgttctgt cgttcgcgga gaaggagctg
     5281 ccgcctggag ggcgtgaaaa agtgatggcg agctttgagc gtgtactgat gcccggtctc
     5341 gagaaaaatc agtacagcat cctgtgggtg gagcaccagg acaagggacg gcttgagctg
     5401 aattttgtca ttccgaacat ggagctacag accggaaaac gcctccagcc gtactacgac
     5461 cgggcagaca ggccgagaat tgatgcctgg cagacgctgg tgaatcacca ttacggtctg
     5521 catgacccca acgccccgga gaaccgccgg acgctgaccc ttcctgataa cctgccggaa
     5581 acgaaacagg cgcttgctga gggcgtcaca cgcgggatag acgcgcttta
//
