LOCUS       NZ_CP129807            10061 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020C08-162 plasmid p2020C08-162-10k,
            complete sequence.
ACCESSION   NZ_CP129807
VERSION     NZ_CP129807.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281338
            Assembly: GCF_030490105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 31x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:07:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,981
            CDSs (total)                      :: 5,857
            Genes (coding)                    :: 5,739
            CDSs (with protein)               :: 5,739
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 59 of 118
            Pseudo Genes (incomplete)         :: 58 of 118
            Pseudo Genes (internal stop)      :: 12 of 118
            Pseudo Genes (multiple problems)  :: 10 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129807.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020C08-162"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020C08-162-10k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            join(8726..10061,1..215)
                     /locus_tag="QYQ37_RS29810"
                     /old_locus_tag="QYQ37_29810"
     CDS             join(8726..10061,1..215)
                     /locus_tag="QYQ37_RS29810"
                     /old_locus_tag="QYQ37_29810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_301743309.1"
                     /translation="MRTQIITCCKPANLFGIAWCAGGLVAGVLLARLLAFLLLDGHFAA
                     ADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNALADFSTEQARKQQAVFLGKD
                     EQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRCVRDFGDAVVYFDPKGDAWAP
                     HVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLSETGDVADHYRISEQ
                     KAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVA
                     GIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLS
                     KYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQE
                     SAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAV
                     MTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            215..946
                     /gene="mobC"
                     /locus_tag="QYQ37_RS29815"
                     /old_locus_tag="QYQ37_29815"
     CDS             215..946
                     /gene="mobC"
                     /locus_tag="QYQ37_RS29815"
                     /old_locus_tag="QYQ37_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            953..1483
                     /locus_tag="QYQ37_RS29820"
                     /old_locus_tag="QYQ37_29820"
     CDS             953..1483
                     /locus_tag="QYQ37_RS29820"
                     /old_locus_tag="QYQ37_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1510..1689)
                     /locus_tag="QYQ37_RS29825"
                     /old_locus_tag="QYQ37_29825"
     CDS             complement(1510..1689)
                     /locus_tag="QYQ37_RS29825"
                     /old_locus_tag="QYQ37_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1715..2143)
                     /locus_tag="QYQ37_RS29830"
                     /old_locus_tag="QYQ37_29830"
     CDS             complement(1715..2143)
                     /locus_tag="QYQ37_RS29830"
                     /old_locus_tag="QYQ37_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2307..2621)
                     /locus_tag="QYQ37_RS29835"
                     /old_locus_tag="QYQ37_29835"
     CDS             complement(2307..2621)
                     /locus_tag="QYQ37_RS29835"
                     /old_locus_tag="QYQ37_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2609..2887)
                     /locus_tag="QYQ37_RS29840"
                     /old_locus_tag="QYQ37_29840"
     CDS             complement(2609..2887)
                     /locus_tag="QYQ37_RS29840"
                     /old_locus_tag="QYQ37_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(3062..3427)
                     /locus_tag="QYQ37_RS29845"
                     /old_locus_tag="QYQ37_29845"
     CDS             complement(3062..3427)
                     /locus_tag="QYQ37_RS29845"
                     /old_locus_tag="QYQ37_29845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(3424..3795)
                     /gene="tolA"
                     /locus_tag="QYQ37_RS29850"
                     /old_locus_tag="QYQ37_29850"
     CDS             complement(3424..3795)
                     /gene="tolA"
                     /locus_tag="QYQ37_RS29850"
                     /old_locus_tag="QYQ37_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(4069..4314)
                     /locus_tag="QYQ37_RS29855"
                     /old_locus_tag="QYQ37_29855"
     CDS             complement(4069..4314)
                     /locus_tag="QYQ37_RS29855"
                     /old_locus_tag="QYQ37_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4641..6326
                     /locus_tag="QYQ37_RS29860"
                     /old_locus_tag="QYQ37_29860"
     CDS             4641..6326
                     /locus_tag="QYQ37_RS29860"
                     /old_locus_tag="QYQ37_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6336..6593
                     /locus_tag="QYQ37_RS29865"
                     /old_locus_tag="QYQ37_29865"
     CDS             6336..6593
                     /locus_tag="QYQ37_RS29865"
                     /old_locus_tag="QYQ37_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            6678..6827
                     /locus_tag="QYQ37_RS29870"
                     /old_locus_tag="QYQ37_29870"
     CDS             6678..6827
                     /locus_tag="QYQ37_RS29870"
                     /old_locus_tag="QYQ37_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            8311..8763
                     /locus_tag="QYQ37_RS29875"
                     /old_locus_tag="QYQ37_29875"
     CDS             8311..8763
                     /locus_tag="QYQ37_RS29875"
                     /old_locus_tag="QYQ37_29875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301743308.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQAG"
                     /name="hypothetical protein"
     BGC             1..10061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6931..7509)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          216..943
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tgaacaccct gcaacacctg cctgacgggt tcgcggtgat gaccgggctg ggcgtggcgc
       61 ggctggcgtt cagcagcccg ctgcgggttg agcgccggga aatttcgctg aaaaccttcc
      121 cggtgctggc gaaaacggac ccgctggcgg agtacctgcc tccggccgac ggcggcggac
      181 caaaagcgga tgatgatttt gcggggcttt actgatgaca ctggagcagt acaacgtcag
      241 ccacgcgaag cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc gctggctgcg
      301 ggaagagctg tgttccacgg cggagctggt tgcgcgacgg ctggggattg cggcggtaca
      361 gccggtttac cgcttcctgg acagtctggt ggcgaaaggg ctgctggtcc gggcgaaata
      421 cccggttgac ggccgtcagg tcagcgtgtg ggggctgacg ccgcacgggg tggcgttctc
      481 gttcgacgag gacgagccgc tgacggacgt tattccgttt cagccgtcaa gggtgtcggc
      541 ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc ctctccatgg agggccgggg
      601 ggccaccggc tggcggtacc tgcaccgtat ggccctgaaa gggatgaaag tgccggacgc
      661 gctggcggag ctggacggca gaacggtggc atttgaggta gagcgcaccg tcaagagccg
      721 caggcggtat caggaagtgg tgtccgggta tctgttcaac cgccgggcga acggcatcga
      781 tgaaatctgg tacatctgcc ctgaccggcc gacgcttgtc cgggtacagc gggcgattct
      841 ggcggtggat gaaatcgtga atccacagac cggcgaggcc cgcaaaacgg tggagctgga
      901 ccgggagagg ctgttcgcgt gctttaaatt tatgacaacg gagtgaggga ttatggcgct
      961 gtacatggta tttattgagg acgtggaaga caaagacggc gagcgctgca ttatgccaac
     1021 cagcgagcat ctggaagaca tggtggtgct cgcctggcag accggggcgt atgttttctg
     1081 ttttgtcgac cgggcccagc atctggcgat gatgaaatcc atcgggatgt atgacgagcg
     1141 gctcaacagc gacgaaaaag ccacgtactt tttcaccggt gaaaatccgc aatgggaacg
     1201 ggacaaaccc acaggaatga agctgtgtga ttacctctat cgaaaggtgg agcggttcgc
     1261 catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc agattaacgc
     1321 gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc
     1381 ggagctgatg cgggaatata acagccgcta ttttaacgtg ccggtgcctg aactgccgga
     1441 ggacaaaccc gcccgccggt ggtggccgtt cggcaaaaaa taacgccagc accggcatgg
     1501 ccggtgctgt tattcttccc cgagcttatc ccgcatcacc tgccagagtg tttccgcatc
     1561 ctcgtgaagc cgttcgcaca tgtcggccgg ggtatccatg tccagctcgt tcagcttttc
     1621 cagcaacagc agcgactgat ccttaatgaa tttagccata ttgagggcag ttttttcctg
     1681 cttcgtcatg ttctccattc ccttcggtaa gttctcagct aaaccagccg gaccagaagc
     1741 gttttttctt ctgtgtcagt tctgttgtca atctgtccac ttcggcctgg agtttccggc
     1801 gctcatcatc gtgctggcgg cgttcttctt cccgggatgt tttatcttcc agcatcaggg
     1861 tgacagcctg ccggagatcg gccagttcct tttgcattgc cagaagctgt tcctgtggga
     1921 aatttttttg tgtattttca cggtgtgaaa tgccgggaat ttgtttctca gggatattca
     1981 catttccgta tacacggatc agttctgaca catccacgac agggttgttt ttcccgtcac
     2041 gtgacacggt aacctttccc tgttttacat gattgtatag cgttcttctg gtaatgcccg
     2101 cggcttgtgc tgcctgtgaa aggttgagca atgttttcgc cactgtatac ccctgatttt
     2161 atcgggtgtg aaaggtgtgt aatttctcac ggtaaatgta tcgctgaatt ttacccgttt
     2221 ttccgttcag gatgtggttt tctgacgcgc tgcgcttgtc aaaccggcga cggccacgca
     2281 atgaattgcg cccccgtcat cggttattag aggctgtctg cccccggata cccggattct
     2341 ttctgatgcc tgacggtgac gatcacaatc tggtccattt cccggtcgtg gcggtagagc
     2401 atcacatagc cactgtcgcc aaacccgatc accagttcct tatattccag cggcagaaac
     2461 ggcaccgggc ggccaatgtc tggcaatgtt ttcagttgct ggatagcccg gacgatcacc
     2521 tcacctcctt ttctggctgc cagccgattt ttggttttga gaaagtcctg aaggcgttgt
     2581 aaatcgcgct gtgccagcgc ggaaatcgtt acctgtggca tggcggagca tcctgctcgt
     2641 tctcagttcc ccaggtactt atccaggctt ccgcttcttc cgcagtaagg tgcagacccg
     2701 tttcctgata gtgctgccat gcggcctgtc cgtcccgcag gtactggctg cgcttctctt
     2761 cccggtcgat gtattcggtg attgcctcca gcattagcgc gtgcgctgag cggtgccggt
     2821 catcggccag cgttttcagc cggtccttaa gttcttcatc gagcctgatg gatgttgctg
     2881 atgacataaa agcccctttg tagtcatatg tattacacat gactacttta atccgtggcg
     2941 gcgctatttg ccagaaatac ggcttttgct gcccattttt cagcttcgct tccactaatc
     3001 agggctgcta caggctcact catcatccag gactattgca gcctgcgggt taaaggacct
     3061 gttactggcg gcaaccatca tcattgagct tttgggtggt gactggtcgc ggatcctgta
     3121 agccgtcatt tttcagactg aattcaaaat ttataacctg tcgcattgcc gggtgccctg
     3181 cgggatatag ccagtgcctc attgccgtca gcgtttcccg aaaaaaaagg tgacagccgg
     3241 taagcgacac gttgtccact cgtccatcgt ccagaatatc aaacgtggcg gtcacctgac
     3301 cttcgatgcg ttttgctgcg gcctgtaccg ggtaggttgg ctgcagtgtg gttacgggtt
     3361 ttagtggtat cgcatgtacc ggagtgagga acatggccgc cagcactgtc agagtaaaag
     3421 ctttcatggt ctgaaatcca gaggggcgtt tttgaatatt tgccaggtat tttcatccgg
     3481 tgccggcgga attttggcgt gggtaacggc tgatattgct gctttacaga gtttcggatc
     3541 tcctccttca gcaatggcgc tgttcagagt gccatcacgt tgtatggata tccgcacggt
     3601 gcacattttt cccgcatagg cctctggttc aaacatctgc atttcaacag cggtcttgat
     3661 ggccgcagcg tagctactga ttttagatga cttatccaga gtataaattt tcggcggctg
     3721 ggtggtggaa tgcttacagg ccgtcaggag taaggggctg cacaatgcca gcaggataaa
     3781 gggtattaaa cgcatcgggt tgatgacctg tcagtaaaaa atgttacatg tatatttgtc
     3841 actctttacg gccacgttca tttacataaa cacggcttat cgtatcgttt ttacaccaaa
     3901 cggcccgcca tgcagttgcg tgcataggca tgcagcgggt tacaatttac cgggcgcgtt
     3961 tccggcggtt ttccgggtgg tttgttgcct gttttatccc gtagccgccg gaaacgccct
     4021 cagaccgtct gagcggtgcg ggcaatgcga cgttatggta aatacccctt accggcggcg
     4081 ctgtggctgc caggtgaagg tggtttccgt agcctcgtat ttctcccgac gcatttcctg
     4141 cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc gcacctgttg
     4201 ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg cctgagcgac
     4261 aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg tcatagtggc
     4321 ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc atgccagtta
     4381 gctttcagat gtgtctatac catctgaaat gtaagtgtga tgaatgtctt aaataataaa
     4441 atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggt ggctccgggc
     4501 gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa aacgggagga
     4561 gtaaggtaat aattatactg tgtatatgta cagtatgttg gctttaattt attgttttaa
     4621 atgtcaaaga ggaaaacggt atgagtggtg gagacggtcg aggtccgggt aattcaggtc
     4681 tgggacataa tggtggtcag gccagtggga atgtgaacgg tacgtctggt aaaggtggcc
     4741 cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg acgcatacgc
     4801 cgaatggaga tattcataac tataatccgg gggagtttgg taacggcggg agtaaacccg
     4861 gtggtaatgg tggtaacagc ggcaatcata gcggtagctc tggtggcgga cagtcttcgg
     4921 ccactgcgat ggccttcggt ctgcctgccc tggctactcc gggggctgaa ggactggctt
     4981 tatcagtttc cggcgatgcg ttgtcggccg ccgttgctga tgtgctggct gccctgaagg
     5041 ggccgtttaa gtttggtctg tgggggattg cgatctacgg tgtgctgcct tctgagattg
     5101 caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc attaccggcc gatgcggtga
     5161 cggagactcc ggcaagtact ttaccactgg accaggcgac ggttcgtgtc agacaacggg
     5221 ttgtggatgt ggtgaaggat gagcggcagc atattgcggt tgtcgcaggt cggccaatga
     5281 gtgtccctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc
     5341 cgggtctccc gtctctgcag gtgagcgtac cgaaaggtgt tccgacagcg aaagccccgc
     5401 caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc tggttttaca gccggtggta
     5461 actcccgtga ggccgttatt cgtttcccga aagagaccgg acagaagccg gtttatgtgt
     5521 cggtgacaga tgttcttacc ccggcacagg taaaacagcg tcaggaggaa gaaaagcgtc
     5581 gccagcaggc atgggacgcc gctcatccgg aagaggggct gaaaagagac tatgataaag
     5641 cgaaagctga gctggatgcc gaagataaaa atattgcgac cttaaacagc cgcattgcat
     5701 cgacagagaa ggcgctcccc ggtgcaaggg ctgctgttca ggaagccgat aaaaaggtaa
     5761 aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat gaatatggct
     5821 ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag aatgagaaat
     5881 taaaagcggc tcaggcatct ttaaacgcaa tgaatgaatc cttatccaga gataaggctg
     5941 cacttaccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa
     6001 ataagttaaa tgaggaaaag aacaagcctc gcaaggggac taaagactac ggccatgatt
     6061 atcatccagt acctaaaaca gaagacataa agggacttgg agagttaaag cgaggtgatc
     6121 ctaaaacacc taaacaaggt ggtggtggta agcgggcccg atggtatggc gataaaaaac
     6181 gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtatcgt gccagtgatg
     6241 gcgaacacct aggcgcattt gatcccaaaa cgggtaagca ggttaaaggg ccagatccaa
     6301 aacggaacat taaaaaatat ctttgagagg taaatatggg gcttaaatta cgtttgacat
     6361 ggtttgataa gaaaactgaa gagtttaaag gggaagagta ttctaaagac tttggtgacg
     6421 atggttctgt cattgaaagc ttgggaatgc ctttaaagga taatattaat aatggtgagt
     6481 ttgatttgaa gaaagaatgg gttcctttac tacagccatt ttttaaaaat caaataaaac
     6541 ctgatgtgga taattgttat atagcttttg attatcggga cggtaactgg taacaaaaaa
     6601 ttatcaagga tgatttgaaa tactgacttc cggtgttcag ggatgaatgc cggagtcttt
     6661 aaatacaggg aaagtatatg aaaaaggtaa agactatttt tctttttata ttgattgttg
     6721 ctggctttct tctggccgca tgtcaggcaa attatatccg ggatgttcag ggcggaacgg
     6781 tggcaccatc gtcctcctct gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg
     6841 atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg aaaaaaccac
     6901 ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac cgtggtaact
     6961 ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac tttggcgcac
     7021 acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc agttaccaat
     7081 ggctgctgcc agtggcgttt tgcgtgcttt cccgggttgg actcaagtga acagttaccg
     7141 gataaggcgc agcagtcggg ctgaacgggg agttcttgct tacagcccag cttggagcga
     7201 acgacctaca ccgagccgag ataccagtgt gtgagctatg agaaagcgcc acacttcccg
     7261 caagggagaa cggcggacag gtatccggta aacggcaggg tcggaacagg agagcgcaag
     7321 agggagcgac ctaccggaaa cggtggggat ctttaagtcc tgtcgggttt cgcccgtact
     7381 gtcagattca tggttgagcc tcacggctcc cacagatgca ccgaaaaagc gtctgtttat
     7441 gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc accggcgcgg ccctgccgtt
     7501 ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc
     7561 cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgagcg aggaggcgca
     7621 atttccccgg cacctgcaag acggtcaccg gacgcgctga tgtgggatat tgtcccctga
     7681 ttaagtcttt ctattccctg acgatcaatt atcaggatag gtgaaccatg tcggtttttc
     7741 gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttgcgga gaaatgcaag
     7801 tcatccccgc tggggcgtgg ctgacttcag gggaaaagac cttgcttacg ctggctggac
     7861 gccaccgacg cacggtcttt tcccctcttt ctggctctgt ccggctggac gccggccacg
     7921 ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa atccacggtg
     7981 ctggacgcac ctgacgaagc agcaaagctg ctcctgattt tgcgggtggt cagccgctcg
     8041 ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg gtgccgcctt
     8101 ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt ttccgcatgg
     8161 ggcggaaaga ggctggcctc cgcggtgaaa gctgaacgca atcaccacgg acgtgaccgc
     8221 cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcgc atcacgccgg
     8281 taaacccgtc gtctgaagga gagagattgc atgtttaata cggattcgct tgcctggcag
     8341 tggcatatcc cggagctggc ccggcagatt tacccgtggg tcagctggct gtaccgggag
     8401 tattacagtc tggccggttt ttttctggtt atcggcctgt ttttcggtct ggctgaactc
     8461 tgggccggcc gaatgcgttt tgtggggctg agccataagg cgcggcggga gtatgccgat
     8521 cgctataagc gcgataataa ccggactgtg ccattttcgg tgctggcctc gctggattca
     8581 cccgggcgga tggtcagggc acggcgacat gtctggaact acgcgtactg gctgaccggc
     8641 gctgccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga gagctgggga
     8701 gaaaccgtcc tgcgcccgga cctctatgcg aacgcagatt attacctgct gcaagccggc
     8761 taacctgttc gggatagcct ggtgtgccgg agggcttgtc gccggcgtcc ttctggcgcg
     8821 gctgctggcg ttcctgctgc ttgatggtca ttttgcggcc gctgacgagg cggtgaacgc
     8881 gaaactgaat caggagagcc ggcgcagcag ccagcggacc ggagaaatga cagacgtccg
     8941 ccacctgcat ttcggggagc cggtaccggt taacgcgctg gccgattttt ccacggagca
     9001 ggcccggaaa cagcaggcgg tgtttctggg taaggatgag cagggccagc cggtgctggt
     9061 ccctcgtgat acctggcgca agaccaatat tcagatcctc ggcctgcccg ggagcggcaa
     9121 gagcgcgatg gccaccaacg cgctcattcg ctgcgtacgc gatttcgggg acgctgtggt
     9181 gtacttcgac cccaaagggg acgcgtgggc gccgcacgtc ttccgggcgc actgcccgga
     9241 cttcaccctg ctggatctgc ggccggggaa accggcgcag ctgaatctgt tccgcgatct
     9301 cgaccagtac gccctgaaga atctgctggt tgccgggttt aacctgagtg agaccggcga
     9361 cgtggcggac cactaccgca tcagcgagca gaaagcggcg aagctgattg cggagcagtt
     9421 cccgcagggg gcaaacattc agcaggtgct ggcggcggcg tacgcgttgc cggaggccct
     9481 gaaaaaggac gtgaaggggc tgatcaccaa gctggagaac gtggccgacc tgagcgtcct
     9541 gcagacggac agcggtatcg atgtggcggg gataatcaac ggcggcggct gcctgtacgt
     9601 catcggctcg atggacgatg aggcggtgat ccgggtgcag aagatgctgt ttgcccgctg
     9661 cgcgcaaatc atcattgcgc gggatgagtt ccggaggtgg ccgcacgcga gcatcatgct
     9721 cgatgagatt aaatatctgc tgtcgaagta cgtgctgaac gcgctgggga cgctgcgcag
     9781 ccgggactgc aacctgctgc tcgcgcacca gtcgctgggc gacttcggtc agtgcgggca
     9841 ggatctgccc gctgattttg tgaaaaccac ggtactggat aacacgccga tccgctggtt
     9901 ctatcgcgcg gccagccagg agtccgcgca gtgggcggcg ggccagaccg gggagatccg
     9961 ggtggacgtg gagcgccgcc gggccagccg ggaggcgggg aacgtggagc acatcagcgg
    10021 cgacagcttc atccagaagg aggcgcggcc gctgtttgac g
//
