LOCUS       NZ_CP129783             5223 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020C07-160 plasmid p2020C07-160-5k,
            complete sequence.
ACCESSION   NZ_CP129783
VERSION     NZ_CP129783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281327
            Assembly: GCF_030490005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 216x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 20:56:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,935
            CDSs (total)                      :: 5,811
            Genes (coding)                    :: 5,688
            CDSs (with protein)               :: 5,688
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 61 of 123
            Pseudo Genes (incomplete)         :: 63 of 123
            Pseudo Genes (internal stop)      :: 12 of 123
            Pseudo Genes (multiple problems)  :: 13 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020C07-160"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020C07-160-5k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            complement(112..434)
                     /locus_tag="QYQ43_RS29645"
                     /old_locus_tag="QYQ43_29645"
                     /pseudo=""
     CDS             complement(112..434)
                     /locus_tag="QYQ43_RS29645"
                     /old_locus_tag="QYQ43_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(<664..807)
                     /locus_tag="QYQ43_RS29650"
                     /old_locus_tag="QYQ43_29650"
                     /pseudo=""
     CDS             complement(<664..807)
                     /locus_tag="QYQ43_RS29650"
                     /old_locus_tag="QYQ43_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            1741..2166
                     /locus_tag="QYQ43_RS29655"
                     /old_locus_tag="QYQ43_29655"
     CDS             1741..2166
                     /locus_tag="QYQ43_RS29655"
                     /old_locus_tag="QYQ43_29655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301743148.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGGSRYSRGQISMDVRICLQCR"
                     /name="hypothetical protein"
     gene            complement(2344..3462)
                     /locus_tag="QYQ43_RS29660"
                     /old_locus_tag="QYQ43_29660"
     CDS             complement(2344..3462)
                     /locus_tag="QYQ43_RS29660"
                     /old_locus_tag="QYQ43_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_117064210.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGEISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
     gene            complement(3938..4153)
                     /locus_tag="QYQ43_RS29665"
                     /old_locus_tag="QYQ43_29665"
     CDS             complement(3938..4153)
                     /locus_tag="QYQ43_RS29665"
                     /old_locus_tag="QYQ43_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064147933.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4160..4657)
                     /locus_tag="QYQ43_RS29670"
                     /old_locus_tag="QYQ43_29670"
     CDS             complement(4160..4657)
                     /locus_tag="QYQ43_RS29670"
                     /old_locus_tag="QYQ43_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4698..5126)
                     /locus_tag="QYQ43_RS29675"
                     /old_locus_tag="QYQ43_29675"
                     /pseudo=""
     CDS             complement(<4698..5126)
                     /locus_tag="QYQ43_RS29675"
                     /old_locus_tag="QYQ43_29675"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(544..623)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.296"
                     /coverage="93.0"
                     /evalue="6.62e-29"
     Typing          954..1474
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.412"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(249..391)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.667"
                     /coverage="99.0"
                     /evalue="2.12e-23"
     Typing          complement(3823..5222)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.786"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cggacttctt ccggcttccc ctgcaggacg ctggcacctt cacgctgccg gtctttaccc
       61 agaagatagt ccaccggacc ggcgcccccg ccgcgacccc ggggatggaa tttaacaatc
      121 atcgtccggc cctttttcct gtacggcatg ccgcaaccgt tccagaccgg catcgatcgc
      181 catcagcgct gccacgatct gcacccggtc atgaccggca ccaccggcgt tcactttccg
      241 ggcgattctg gttcaggtta ttgcccatgc cggcaagctg acgcagcagc gccggcgaaa
      301 tcgaggggag cctgccggcg cgtgtcggtt tctcatcaag gcaggtctgg cgcatccacg
      361 ccgccagttg ttttccgtcg caccgctcaa agcaaccgcc ggtgttcgtc ttccgtcacc
      421 cacatcgtga gcatcttgtt gcgtttatct gccagaatcg cctcctgatt aaccccgtga
      481 cggacgggaa agcctttccc ggtcggcgcg gggttggaca agccgcaggc gcgtcagtgg
      541 tttttagcgg gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
      601 ctggcttact atagtggcat cggtatgcac gaacgatccc gttcaggtcc gactgctgcg
      661 ccttatgccg gtaagctgac gcagcagcgc cggcgaaata tcgtcttgag ttttccaatc
      721 aaggcaggtc tggcgcatcc aagtttttcc atcgcaccgc tcaagcaacc gccggtgttc
      781 gtcttccgtc acccacatcg tgagcaagtt gcgtttatct gccagaatcg cctcctgata
      841 atgacatggg tatcagcgaa gcgctagtgg agtgtatagc cttactatat tgagcagttc
      901 ggtatgaagt atcagcgaaa gtgctatata tggcagggat gacatgcagc gacggtgcgt
      961 aaggcagata atggtagtgc cgggggtatg acgcctcctc gctcactcgg tcgctgcgct
     1021 cctgccgtga gactgcggcg ggcgttacca gctcacgagt gacgagagaa agaaatagcg
     1081 ctcacgtcag gaaggccaga aaaaaggatg cggcgttgcc gtttttccat aggctccgcc
     1141 cccctgacga gcatcacaga atctgatgct caaatcagtg gtggcgaaac ccgacaggac
     1201 tataaagatt cccaggcgtt tccccctggt agctccctcg tgcgctctcc tgttcctgcc
     1261 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
     1321 actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     1381 gtccgactgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc
     1441 aaaagcgcca ctggcagcag ccactggtaa ctgaaaaggt ggatttagat attgagagtt
     1501 cttgaagtgg tgacctgact gcggctacac tagaaggaca gtatttggta tctgcgctcc
     1561 accaagccag ttacctggtt aagcagttcc ccaactgact taaccttcga ttaaaccgcc
     1621 tccccaggcg gttttttcgt ttcaagaggc tacgcccaaa cgacaatgat cgtcaaggat
     1681 cttcttggta agatatgaca aagagctttc gaacttttat atcttattca aggaactaag
     1741 atgcaagaca ggtcagtagt taatcgacag ggatttaacc cagagatcga tctgccgtat
     1801 tcgctaagga ttagtgattt tgagcacgcg atggaagata tttatgactt cttccatgat
     1861 gtgaacaact tactatcaaa caaaggtttg cataggcttg atgacatgat gagaccagca
     1921 gctatgtctg gcctcatatc agatatgatc acagcttccc tcgctaaatt ttcaagatcg
     1981 cttgttgaaa acaagcattt caatggacat ccagacctag ttgttagggg caggtatccg
     2041 aatgacagta tctcctcagg agaagaaggc attgaaataa aaaccacccg gaaaaatggg
     2101 gggggcagtc gatactcacg gggccagatc tcaatggatg tgcgtatttg tctacaatgt
     2161 cgataatgag accgaaccgg cctcagacag aaagtcgatg aaatttaccg aggtatatct
     2221 ttgccacgtt gatgaaaatg acttccgtcg taatgatcgt ggtgcattag gaaccagaac
     2281 agcaaccctt cacaaagaag ggttgaagaa gctcagagaa agctgggttt acaaggactt
     2341 agtttagtta ttttccttgc taggaagagc tgatagcgcg gggatagcct tctccgccaa
     2401 atcaaagaac tctcggtcta tttcaactcc aatgctctca taacctatat gatttgcagc
     2461 tgccaatgtg gagcctcccc ccataaatgg atcgagaata atcccctctc ccaaaggaag
     2521 cactgccctc actaattgtc gcatgaaatt ttgcggcttt aatgagggat gtggtgctat
     2581 ctttttttcg tgtgctggcg tcggatgaga tttgatgacg tcgccaaatg gcctatcagc
     2641 tgaaatcctc ctgaatccac ctgttttcca ctttcttaga ttatcctgaa cccttccatc
     2701 tagaggcttt ctcaacacta tccatggttc ccattgagaa cgaggcatga cagaaacatc
     2761 tccaaattca tcatgggcgt ttttgggtct atccccacct ctcatggtca taacctgacg
     2821 tacaatgtaa cctcgtagct ctaagccaga cgtagacata gagtccgcga ctatatggga
     2881 tagtagaggg ttggatgcga taactatatt cccgccagga acaaccaccc tttcgacttg
     2941 cgaggatagt tctttgaaaa ataatgatat atttctgagg tcttcttccc gcaaaaccgt
     3001 aaagcgaggt aatggcgctc gcttatgtcc atcaaaggaa ggtggctttc tccaaatacc
     3061 acctttccct gcccttagtt tctcagtctg ttgttttgta tactcgacta atccgtaagg
     3121 aggatcagtt acaactgcat gaattgaatt atctttttgt ctttttaacc attcaattgc
     3181 atcgtcaaaa aaaatcgtcg atttcccaac cgtcttaggt gaagacgtca aatctaggga
     3241 gggagtaata ccagcatcac catttaagcg atagacacct cggtctactc gctcgaacag
     3301 acctggggta tttaaactca gatatgagcg aacagaagac tttgaaattt ctccaatttt
     3361 taaataaaca gcttttgtta tctcctcaat agaagcttct cttccaagcc ttaaaaaaca
     3421 ttcaattatc gagtcacgaa caacaccagg agccaaagac atgtttataa atccagtatt
     3481 tttatataaa ataataatac taactagacg tcaggacgtc aatttagttt aaaagagacg
     3541 tcaaaaaagt atacattaac ttttttttta ccataacatc ccttacccgc accgcgccgg
     3601 aggccctcag ggcgtttctg atggctgacg ggcaaaaggt ggtggcaagt acaccgaaaa
     3661 ccaccagaaa cgcacctgat ttattttacg catatgcatc acttattcac atcactgcat
     3721 agccggtgat ttcggttaaa aaagactctc tgtgtgctga aatcgcagaa aatggccatg
     3781 atccccgtca cgctcaggcc acctgagacg ggcgtttatc agtctccata ctccctgtca
     3841 ccatgagata atgtgcgttg ccgttccggt accagatctg ctgtcttctc tggttccttt
     3901 tccgttcttt gcccgtatcg ctgttgctca atctcattca gcctgtcgat aaccggcgca
     3961 gctgctcgct caaactctgc acctgcctgc tcaagcccga cacgcgctcg ctcagcgccg
     4021 cgttctcccg ttgcataaga ccagacatct tccgccattc cccgaaggcg ctctcccact
     4081 cgtccagcct tctcgagtag tcctgctgta gctgctctaa cgcgctcagc aattgttttt
     4141 ccagttccgt cattctgctc tactcccgta ccagcttcac cgcgttccgc gtcttgttgt
     4201 ccaccgtccg gcccgtttcg gctttcatcc cctccggcag caccagaaac cgtccgttgc
     4261 ggttctccag ataggtcacg ccccacgtct gcatgtgcag tttcgacagc gtatctctct
     4321 gctgcctgat ttccgccagg ttcgccgcta tccggttccc ctggtaccac agcaccccgc
     4381 tcaggctggc gaacagcagc cctgacacca tggtgatggt cagccacgtc cgcccgacca
     4441 ttcgcagcac gctcagccgg ttcgactgca tcgccgcgtt catcccctgt tcgtgggcgc
     4501 tgatggcgtc gttgattctc ttcgcgcttg cgttcagttc cgatttgaca acgttctcgt
     4561 gcttgctgaa tgcggctttc agcctctctt cggtaagctg ctgctgtgct ttcgatttct
     4621 cctcgaagtc cctcgaggcc gttaaaagac tgctcataaa tggctcccct cagtcggata
     4681 tttcgccccc cgtccgggtc ggcaatgctg atactgtttt ttgtggttct gaccacctca
     4741 aaaccggccg ctgtgagcgc ccctgtgacg tcatcacgcg ttttcagctc tcctgatgag
     4801 gctaacatca gcaaaccccg tgtaatcgtc tccgcggctt cctgcttcgt ttctggcagt
     4861 cctgacggcg tgaccagcgc ccgccggttc tccgggtcgt tcggatcatg cagccccagc
     4921 ctgccgttga ccactgtctg ccacgcatcg atgcgcggcc ggtcggcacg atcgtaatac
     4981 ggctggaggc gtttccccgt cagtaattcg gtattgggga tcagaaaatt cagctcaaga
     5041 cgacccttgt ctgcgtgttc cacccacaga atgctgtact gatctttatc gagtccgggc
     5101 atcagaaccc gctcaaagct ggccatcagt ttttcacgct gacccggcgg caggtccgcc
     5161 tccgcgaatg acagcacccc ggaggtatat tttttcacat acggcgacgc atcaatcagc
     5221 tcc
//
