LOCUS       NZ_CP129774             4562 bp    DNA     linear   CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020C07-038 plasmid p2020C07-038-4k.
ACCESSION   NZ_CP129774
VERSION     NZ_CP129774.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281324
            Assembly: GCF_030490065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 22x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:29:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,655
            Genes (coding)                    :: 5,513
            CDSs (with protein)               :: 5,513
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 46 of 142
            Pseudo Genes (incomplete)         :: 94 of 142
            Pseudo Genes (internal stop)      :: 11 of 142
            Pseudo Genes (multiple problems)  :: 9 of 142
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129774.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020C07-038"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020C07-038-4k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            1257..1433
                     /locus_tag="QYQ60_RS28865"
                     /old_locus_tag="QYQ60_28865"
     CDS             1257..1433
                     /locus_tag="QYQ60_RS28865"
                     /old_locus_tag="QYQ60_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1423..1767
                     /locus_tag="QYQ60_RS28870"
                     /old_locus_tag="QYQ60_28870"
     CDS             1423..1767
                     /locus_tag="QYQ60_RS28870"
                     /old_locus_tag="QYQ60_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1976..>2410
                     /locus_tag="QYQ60_RS28875"
                     /old_locus_tag="QYQ60_28875"
                     /pseudo=""
     CDS             1976..>2410
                     /locus_tag="QYQ60_RS28875"
                     /old_locus_tag="QYQ60_28875"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2445..2954
                     /locus_tag="QYQ60_RS28880"
                     /old_locus_tag="QYQ60_28880"
     CDS             2445..2954
                     /locus_tag="QYQ60_RS28880"
                     /old_locus_tag="QYQ60_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2961..3173
                     /locus_tag="QYQ60_RS28885"
                     /old_locus_tag="QYQ60_28885"
     CDS             2961..3173
                     /locus_tag="QYQ60_RS28885"
                     /old_locus_tag="QYQ60_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3840..4064
                     /locus_tag="QYQ60_RS28890"
                     /old_locus_tag="QYQ60_28890"
     CDS             3840..4064
                     /locus_tag="QYQ60_RS28890"
                     /old_locus_tag="QYQ60_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4048..4314
                     /locus_tag="QYQ60_RS28895"
                     /old_locus_tag="QYQ60_28895"
     CDS             4048..4314
                     /locus_tag="QYQ60_RS28895"
                     /old_locus_tag="QYQ60_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          1092..1171
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(509..906)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1758..3289
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggttaataa gatcacttag actcattttg gttttattcg aaagccgttg taaggattgc
       61 tttaatccta tatctagcat gatatttgtt ctttctctca ttaccccccc ttgctattgc
      121 gttttttgtt gcgcacaaag ctaccattgc gcattaagaa acgcaataaa aaaggaggga
      181 gggagtggtt tgtacatgat gtgcagtgcg ctgagggcat cagtggcggg tgtttttccg
      241 tgggatggat ggagctccgg ggataacaga actgacgatc atttaaagga tcttcttgag
      301 atcctttttt tctgcgcgta atctcttgca ctgtaaacga aaaaaccgcc tggggaggcg
      361 gtttgatcga aggttaagtc agttggggaa ctgcttaacc gggtaactgg gtatacgaga
      421 ccgcagatac caaaactgtt ctttcagtgt agccgcagtt aggcacccac ttcaagacct
      481 ctcaatatct ctctcgtata tatccagtta ccaatggctg ctgccagtgg cgtttttgcg
      541 tgtccgtccg ggttggactc aagatgatag ttaccggaaa gggcgcagca gtcgggctga
      601 acggggggtt cgtgcataca gcccagcttg gagcgaacga cctaaaccga gccgagatac
      661 cagcagcgtg agctatgaga aagcgccacg cttcccgaaa gggaaaaagg cggaacaggt
      721 atccggtaaa cggcagggtc ggaacaggag agcgcacgag ggagccgccg gagagaaatc
      781 accggcatct ttaagtcctg tcgggtttcg tcctcctctg acttgagcgt ccatttctgt
      841 gatgctcgtc aggggaggcg gagcctatgg aaaaacggca acgccgcatc cttttctccg
      901 gccctttctg atttgagcgc ctttgcttta tcccgttatc cgtgagtcgg tcacgcccgc
      961 cgcagccgaa cggcaggagc gcagctaccg agtgagcgag gaggcgtcat ttcatctgtc
     1021 cgtgcatggt attgcggacg ctcctgagcc acatatcaca cctgccacat gaggcacttt
     1081 catgaatcgt gctctcatgc caacatagta agccagtata cacagcgtaa acgctgtgac
     1141 tggttcaggg ctgcgccccg aaacccgcta acaccactga cgcgctggcg cttgtccaac
     1201 gccggagcga cccggaaaac ttttccgtgc cgtccggcgt ttgcgaagga gggataatgc
     1261 agaagccgaa gaagaagccg ctcgtcgtaa aaaaaagatc ctggcatccg gcagtgatgg
     1321 caaatctgat gcgtcaggca caggaggaag attaccgccg ggagcagatg gcagagcttg
     1381 agcgttcagc ccggtcacat tttgcggata tcggaggaga ggatggcggt taagcgtacc
     1441 cgttttttag gtatccgggt gaccgatggg gagtaccagc agctgctgga gcgctgcaat
     1501 ggaaggcaac tggcggtgtg gatgcgggaa acctgtctcg atacgcgccc ggcgcggtcg
     1561 ttgcggctgc cgtccatcga tccggttttg ctgcgccagc ttgccgggat ggggaacaac
     1621 ctcaaccaga tcgcccggaa aattaacggc ggccagtggt caggcgctga ccgggttcag
     1681 gtggtggccg cgctgatggc cattgatgcc gaactcgagc ggctgcggca tgccgtgctg
     1741 gaaaaggggg cagacgatga tcgttaagtt tcatccgcga gggcgaggcg gcggtgccgg
     1801 gccggtggat tatctgctgg ggaaagaccg ccagcgcgac ggggccatcg tcctgcaggg
     1861 aaagccggag gaggtccggg agcttatcga cgcctcgccc tacgccaaaa agtacacctc
     1921 cggcgtcctg tcttttgctg aacaggattt accgcccggc cagcgtgaaa agctgatggc
     1981 gagcttcgag cgggttctga tgcccggact cgataaagac cagtacagcg tgctgtgggt
     2041 tgaacaccgg gacaaggggc ggctggaact gaattttctg gtaccgaaca cggaactgct
     2101 gaccggcagg cgtctccagc cgtactacga ccgggcagac cgtccgcgca tcgatgcgtg
     2161 gcagaccgtg gtgaacgggc gactggggct gcacgacccg aacgcaccgg agaaccggcg
     2221 cgcgctggtg acgccatctt cgttacccaa aacgaagcag gaagccgcag aggctattac
     2281 gcggggctta ctggccctcg cctcgtcagg ggagcttaaa acgcgtcagg acgtcactga
     2341 ggcgctggaa agcgcaggtt ttgaggtcgt gcgcaccaca aagagcagca tcagcattgc
     2401 cgacccggac ggagggcgaa acatccgact taagggagcc atctatgaac agtcttttaa
     2461 cgctggcgaa ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgaaatgc
     2521 tgaaagccgc attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcggga
     2581 agagaatcag cgccgccatc cgcgcccacg agcagggtat gagcgcggcc atgcagtcga
     2641 accgcctgag cgtgatgcgt atggtcgggc ggacatggct gaccatcacg atggtgtcag
     2701 gactgctgtt cgccagcctg agcggggtgt tgtggtatca ggggagcttg atagcgtcaa
     2761 atctggccga aatcgaccgg cagaacgcag cgctgtcgaa gctgaacgcg aagacctggg
     2821 gcgtgacgta cctggaagac agcaacggac gttttctggt gctgccgaag gggacggccg
     2881 tagacaggac gcagagctgg acggtcggga acgggcggtc gaagcagaac gcgctgagac
     2941 tggtgaagga gtaagtgccg atgaccgagc tggaaaaaca gttgctgagc gcattagagc
     3001 agctacagca ggactactcg aaaaggctgg acgagtggga gagcgccttc ggggaatggc
     3061 ggaaaatgtc agggcttatg caacgggaga acgcggcgct gagcgagcgc gtgacgcgct
     3121 tgagtcagca ggtggaacgc ttgagcgggc aactgcagcg tttgagccgg tagtgcagcg
     3181 tcatgagatg gccgtggccg cagaacgggc gcacgagcaa cggcagcacg aaaaggaact
     3241 gacggaagcg cgttcccggc agcgaagcta tgacggtccg tcgctgggct gaaatgcagg
     3301 aaaacagggt tctcatcggg catttacgct gaaaccggat gtcattcacc tggctgcatg
     3361 gctatgcagc caggtaaaat ttatctgtcg cgaacggggc ggtttatcgg gtggtttgtt
     3421 gccggtttta cctgactgcc accccgttcg cggcgaaccc gtccggggcg gtgcgggcaa
     3481 cgggtgttat gtttaatcag cgtgaacggc agatacaaaa aagccccgga gggcttttaa
     3541 ctactgactt atttttttga agatttgctg ggtttcaatt tgtcagtatt tcccggcgtt
     3601 tttttattgt cacgctggcg tttatcaggt tcccctgttg aatcggcaat tcgtttcgga
     3661 cccggcttaa gacccataat cgttatcctt tcaatagtta agaggaaagc cctcaagcta
     3721 gatatattga ccgcgttacg gattatcaag aggggctaac aagattattt tttgttcttt
     3781 taggttgatt gtggtactgt tgatatacat gtatagcaaa tggtgttcgg gaggtttata
     3841 tgcttgctat ccggttatct gatgagattg agtcccgtct ggactcgctg gcgaagcaaa
     3901 ccggcagaac aaagacgttt tatgcgcggg aagcaatact ggcgcatctg gaagacctgg
     3961 aggattatta tctttcagca gaaactgctg cacgcgttcg ccgtggtgat gaagcagtgc
     4021 attcgtctga agacgtgagg aagtcacttg gtctggacga ttaactattc cgatcgggcg
     4081 ctcaaatcgt tacgcaagat ggacaaacag aacgcacgac ggattgtgga ttttatgagt
     4141 ttacgcattg cagttgctgc cgatcctcgc cagtcaggga agccgctcaa aggtgagctg
     4201 ggcgagttct ggcgctatcg ggtgggagat tatcgcgttc tgtgtgagat ccgagatgac
     4261 gagcttgtta tccttgccgc cacgattgga catcgccgcg aagtttacga ctgaagcccg
     4321 ctatgcgggc ttttttgatg ccgacaacag gtattatggt aaatactcga ctaactacgt
     4381 gttctactgt ttgtcggttt tagttaacca gcaggttgtc ttaacgataa tgtagccgtt
     4441 atttttaact aataaggtca tattgttaac gtcaggcata ataaaagaca ttatggcctc
     4501 agagttatca tcagaagcag ctctctgcat tactacttcg attccctttt ctttgaatct
     4561 at
//
