LOCUS       NZ_CP129769             8191 bp    DNA     linear   CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2020C04-115 plasmid p2020C04-115-8k.
ACCESSION   NZ_CP129769
VERSION     NZ_CP129769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281311
            Assembly: GCF_030489765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 241x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 20:51:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,982
            CDSs (total)                      :: 5,858
            Genes (coding)                    :: 5,715
            CDSs (with protein)               :: 5,715
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 74 of 143
            Pseudo Genes (incomplete)         :: 67 of 143
            Pseudo Genes (internal stop)      :: 13 of 143
            Pseudo Genes (multiple problems)  :: 11 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129769.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020C04-115"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2020C04-115-8k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2020"
                     /collected_by="National Health Research Institutes"
     gene            complement(67..1173)
                     /locus_tag="QYQ51_RS29855"
                     /old_locus_tag="QYQ51_29855"
     CDS             complement(67..1173)
                     /locus_tag="QYQ51_RS29855"
                     /old_locus_tag="QYQ51_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="WP_004176140.1"
                     /translation="MSLKQIWQAANPKGHLLTAISFLIPIVCGSGFIIAIGMGLGGTVQ
                     DTLTPGQFDVWQAMATLGAKALGLLPVVIAVGISGSIAGKPGIAPGFVVGLAANTISAG
                     FIGGMIGGYIAGYIALAIIKNVKVPDWARGLMPTLIVPFFASIISCLIMVYIIGTPIGI
                     FTEALTSFLRSMGTSSNLVLGAVIGALCIVDFGGPLNKTCFAFVLTLQAQGINEPITAL
                     QLVNTATPIGFGLAFFIAKLLRKNIYNREEVETLKSAVPMGIVNIVEGSIPIVMNDIVR
                     GIAAAAIGGACGGAVTMVYGADATVPFGGVLMIPTMSHPMAGIMALLVNIVVTATVYAV
                     IKKDIPRDVIVDNDYEEEDIDLDDIKVS"
                     /name="PTS fructose transporter subunit IIC"
     gene            complement(1216..>1434)
                     /locus_tag="QYQ51_RS29860"
                     /old_locus_tag="QYQ51_29860"
                     /pseudo=""
     CDS             complement(1216..>1434)
                     /locus_tag="QYQ51_RS29860"
                     /old_locus_tag="QYQ51_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706300.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA];
                     GO:0022877 - protein-N(PI)-phosphohistidine-fructose
                     phosphotransferase system transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]; GO:0016310
                     - phosphorylation [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /name="PTS fructose transporter subunit IIB"
     gene            1528..2799
                     /locus_tag="QYQ51_RS29865"
                     /old_locus_tag="QYQ51_29865"
     CDS             1528..2799
                     /locus_tag="QYQ51_RS29865"
                     /old_locus_tag="QYQ51_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISPa13 family transposase"
                     /protein_id="WP_000813355.1"
                     /translation="MKQQQDTRFSSAFERFFSAAELDALGKQSGFMKRRRQVTPQKFCM
                     ALVSALGAGTTNSIADVHRQFNHMHSTDIKLKPFHKQLVKMAAPEFMREVFEKALALHL
                     PDMFAIRNKYQDMFKRIILQDGTSFAVHDDLMFYFPGRFNENSPAAVELHVTYDVFKGQ
                     PDGVSLTEDTAPERDYLPSPASLSGCLFMADAGYFSKAYIQQLQEACAYFIMRMCNRVN
                     PMAVCTKTQQMKPLKTWLKELPNAGVLDLDVQWPNGPVYRCVAFAALDHKKRHVLVCTN
                     LDREQFPPAVVGDFYRVRWQIELLFKEWKSMNNLNKFDTGHATIVETLIWGSLLAATLK
                     RWLINAAQKKYNRVLSLFTGAKTAHMWWIPFCFDIAMSRGTDLAKAMNNALAFLAQHCQ
                     RQNVKRDQISGVYKLLNNTNKSLG"
                     /name="IS4-like element ISPa13 family transposase"
     gene            complement(2788..2922)
                     /locus_tag="QYQ51_RS29870"
                     /old_locus_tag="QYQ51_29870"
     CDS             complement(2788..2922)
                     /locus_tag="QYQ51_RS29870"
                     /old_locus_tag="QYQ51_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301696396.1"
                     /translation="MNIVGVTACTVGIAHTYIAQKKIETAAKKAGHNVHTYDLNDLTQ"
                     /name="hypothetical protein"
     gene            complement(2979..3449)
                     /locus_tag="QYQ51_RS29875"
                     /old_locus_tag="QYQ51_29875"
     CDS             complement(2979..3449)
                     /locus_tag="QYQ51_RS29875"
                     /old_locus_tag="QYQ51_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226990.1"
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]; GO:0016310 -
                     phosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_002905131.1"
                     /translation="MDISTVLNVKNIKLNMTARTKEEVIEELTDLLIQDGAVTNKEDFI
                     RDVWLREELGSTGFENHIAIPHGKSSGVSRTALAIGRTQHAIPWETMDGSDVRCVILFA
                     VCLVDQNATHIRLLAQVSGSLADEDIIAKLLVESDPHKIIALFNSETENADA"
                     /name="PTS sugar transporter subunit IIA"
     gene            complement(3566..4246)
                     /gene="alsE"
                     /locus_tag="QYQ51_RS29880"
                     /old_locus_tag="QYQ51_29880"
     CDS             complement(3566..4246)
                     /gene="alsE"
                     /locus_tag="QYQ51_RS29880"
                     /old_locus_tag="QYQ51_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose 6-phosphate 3-epimerase"
                     /protein_id="WP_002905133.1"
                     /translation="MSAKFSPSLMCMDLTQFKEQITAMNKKADFYHVDIMDGNYVRNIT
                     LSPFFIENLKKITTVPIDVHLMVNHPEDIIPMCLEAGADIISFHPETANNKIFRLLNQI
                     KDAGKKCGVVLNPATPAESIAEYAHLLDKVTVMSVDPGYAGQKFIPESLNKIRKLINMR
                     KNNGYNYLTEIDGSCNEKTFGQIAESGVDVFIVGTSGLFSLHEDVSQAWDRMIEIFQRE
                     TVAA"
                     /name="alsE"
     gene            complement(4267..5529)
                     /locus_tag="QYQ51_RS29885"
                     /old_locus_tag="QYQ51_29885"
     CDS             complement(4267..5529)
                     /locus_tag="QYQ51_RS29885"
                     /old_locus_tag="QYQ51_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226992.1"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="WP_004176135.1"
                     /translation="MLHFLIYDVLGTPAILVGLFSLIGLLLQKKGISDVISGTLKTIMG
                     FVILTAGAGIIAYTLTIFSQLFEHSFHIQGVVPNTDAMAALAQKNYGTETATIMVLGML
                     INIALARLTPLKYIFLTGHHTLYMAAMLAVILSVGGLSGGWVVAIGAVILGAMMVISPA
                     ILQPFTRKITNTDDLALGHFGSIGYLLSALVGKIIGKGSPSIEEIKVPKSLNFLRDSSV
                     AISLTMMILFLVLVLVAGKSFVEETLSAGQNFIIFAIIQSLTFAAGVYIILAGVRMVIA
                     EIVPAFKGIADKLVKDAKPALDCPTVFPFAPNAVIVGFLASFVAGLVSMFLCPLFGLSV
                     IVPGLVPHFFCGATAGVYGNITGGRRGAVVGAFAHGLLISFLPAILLPMMGDMGLGSTT
                     FGDADFGVVGIVLGHIIAMFN"
                     /name="PTS ascorbate transporter subunit IIC"
     gene            complement(5560..5826)
                     /locus_tag="QYQ51_RS29890"
                     /old_locus_tag="QYQ51_29890"
     CDS             complement(5560..5826)
                     /locus_tag="QYQ51_RS29890"
                     /old_locus_tag="QYQ51_29890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226993.1"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_004176134.1"
                     /translation="MKKVLIVCGNGLGSSFIVEMNVKKILAEMNKEAEVAHTDLTSAKS
                     ETADLILSAKDIAEHLSSHSAKVVGLSNLLDNNKIKEILAENI"
                     /name="PTS sugar transporter subunit IIB"
     gene            complement(5838..6275)
                     /locus_tag="QYQ51_RS29895"
                     /old_locus_tag="QYQ51_29895"
     CDS             complement(5838..6275)
                     /locus_tag="QYQ51_RS29895"
                     /old_locus_tag="QYQ51_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226994.1"
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]; GO:0009401 - phosphoenolpyruvate-dependent sugar
                     phosphotransferase system [Evidence IEA]; GO:0016310 -
                     phosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="WP_004199922.1"
                     /translation="MIRDWLTPDKIKVINSIDSWENAVRLAAEPLLMQGYIAGSYIEAI
                     LQSHKELGPYYVLAPGLAMPHARPEQGVLRNGLSLLHIKEGVSFGSTENDPVYVVIILC
                     ARSGNEHITMIGELAEIFSDQQKLHRLFNADDSKAIQAVID"
                     /name="PTS sugar transporter subunit IIA"
     gene            6493..7545
                     /locus_tag="QYQ51_RS29900"
                     /old_locus_tag="QYQ51_29900"
     CDS             6493..7545
                     /locus_tag="QYQ51_RS29900"
                     /old_locus_tag="QYQ51_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226995.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]; GO:0000976 - transcription
                     cis-regulatory region binding [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]; GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0006355 -
                     regulation of DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_004151868.1"
                     /translation="MVKSLDADGSPKEKRRKGTGKITLTEVAEFVGVSAMTVSRALRMP
                     EKVNPELRDRIDAAVSQLGYVPNLQARNLASVHSDLILAVVPTFSSPGFLPVSEALQKV
                     LTERGYTMMFMESGHDGQSEQKTFEKMLAYNPAAIIQFNIDTIESCTQLLANVDVPILE
                     IGALNTHPVGMCIGVDYGKAVKQIVTHLADASLKNIALLCTPANNTMFRQLLSGWNTAM
                     LALNRSPHRVVTTHLPSTIATGVNIFKDMMITWGDLDALICTSDEMACGCMMACHSAGI
                     KVPNTVAIASLGGGVLSTVCSPALTTVEFPWHDIGVKAGKALLELLNDKPGEKFIEIPS
                     VLKVRASTAA"
                     /name="LacI family DNA-binding transcriptional regulator"
     gene            complement(7657..7875)
                     /locus_tag="QYQ51_RS29905"
                     /old_locus_tag="QYQ51_29905"
     CDS             complement(7657..7875)
                     /locus_tag="QYQ51_RS29905"
                     /old_locus_tag="QYQ51_29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_014343071.1"
                     /translation="MNYRGALYLLTGLGDIVYPGLALDGLQCKAAQAGRGYEITAWDGW
                     QDKREMRLAVYPLSSEKTLLSRLFFAL"
                     /name="hypothetical protein"
     gene            7969..>8191
                     /locus_tag="QYQ51_RS29910"
                     /old_locus_tag="QYQ51_29910"
                     /pseudo=""
     CDS             7969..>8191
                     /locus_tag="QYQ51_RS29910"
                     /old_locus_tag="QYQ51_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015874815.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /name="oxalate/formate MFS antiporter"
ORIGIN
        1 cttagtcatg ggctgatgtt cttacaggaa gagcttaatg ttgatttaaa ataaccctgg
       61 gatacgttag ctcactttaa tatcatccag gtcgatatcc tcttcttcgt aatcattatc
      121 aacaatcacg tcgcgaggga tatctttttt gatgacggca tagaccgtgg cggtaacaac
      181 gatattcacc agcagggcca tgatgcctgc catcggatgt gacatcgtag ggatcatcaa
      241 tactccacca aaaggaaccg tcgcatccgc accatagacc atggtgaccg ccccgccgca
      301 cgcgccgccg atagccgccg ccgcgatacc gcgcacaata tcattcatga cgatcgggat
      361 agaaccctca acgatattca caatccccat cggaaccgcc gattttaagg tttccacttc
      421 ttcacggtta tagatgttct tacgcagaag cttagcaatg aagaaagcca ggccaaatcc
      481 gataggcgta gcggtgttaa ctaactgaag tgcggtaatg ggttcattta tcccctgcgc
      541 ctgcagggtc agcacgaaag caaagcatgt cttatttagc gggccgccaa agtcaacaat
      601 acacaatgcg cctatgactg cgcctaacac caaattcgac gacgtgccca tgcttctcag
      661 gaaagacgta agcgcttccg taaagatacc gataggcgta ccgatgatat agaccataat
      721 caggcagctg ataatggagg caaaaaaggg aacgatcagc gtcggcatca acccgcgggc
      781 ccagtcaggc accttgacgt ttttaatgat cgccagcgca atataccccg caatatagcc
      841 accaatcatc ccaccgataa agcctgcgct tatggtattc gccgccagcc cgacgacaaa
      901 tcctggcgcg atgcctggtt tgccggcaat cgagccagat attcccacgg caatcaccac
      961 cggcaacagc ccaagcgcct tggcgcccag ggtggccatc gcctgccata catcaaattg
     1021 tcccggagtt aacgtgtcct gcaccgttcc gccaagcccc atgccgatgg cgataataaa
     1081 cccagaacca cagacaatgg ggatcagaaa cgagatagcg gtcagcagat gtcctttagg
     1141 gtttgctgct tgccatattt gttttagaga cataagtcac gccctttaat gtagagtcgc
     1201 caggccgaaa taaaattatg aattgactag ttcactgagt ttttcaatca acttattggg
     1261 ggatttgact gccgtttccg tcgcgacttt aacgactttc ttgccggcaa atctctcctc
     1321 gcccgttact ttgacatcgg cagcaagcag gacaatatct gctgcggcaa tctcatcagc
     1381 ggttaacgag ttctcaatgc cgatggttcc ttgcgtctcg attttaacgt tatgtcatag
     1441 gtatgatctt taggagttga agaggtgctt cattcgtgat ctaattgatt taccagaaaa
     1501 aaaagatcac ctttgcaggc aaaacgaatg aagcaacaac aagatacccg attttcgagc
     1561 gcttttgaac gatttttttc agcagcagag cttgatgcgc ttggcaagca atcaggcttt
     1621 atgaagcggc ggcggcaggt gacgccacag aagttttgca tggcgctggt cagtgcgctg
     1681 ggtgcaggaa caacgaattc gattgccgat gtgcaccggc aatttaacca tatgcactca
     1741 acggacataa agcttaaacc ctttcataaa cagttggtta aaatggctgc tcctgaattt
     1801 atgcgtgaag tgtttgaaaa ggcgctggct ctgcatctcc ctgatatgtt tgctatcagg
     1861 aataaatatc aggacatgtt caagcgcatt attttacaag atggcaccag tttcgctgta
     1921 catgacgact taatgttcta ttttccagga cgttttaatg aaaatagtcc ggcagcggtt
     1981 gagttacatg tcacttatga cgtgtttaag gggcaacctg acggtgttag tctgactgag
     2041 gacacggcac ccgagcggga ctatttgcct tcacccgcct ctttatcagg ctgcttattt
     2101 atggcagatg caggttattt ttcaaaagct tatatacaac agcttcaaga agcctgcgcg
     2161 tattttatta tgcggatgtg taaccgtgtt aatccaatgg cggtttgtac taagactcag
     2221 cagatgaagc cgctgaaaac atggttaaaa gaattaccca atgcaggtgt attggattta
     2281 gatgtgcagt ggccgaatgg cccggtttat cgttgcgttg catttgcagc attagatcac
     2341 aaaaaacgtc atgtattggt ctgcactaac cttgaccgtg agcagtttcc tccagctgtt
     2401 gtgggtgatt tttatcgtgt tcgctggcaa atagaattat tattcaaaga gtggaagtcg
     2461 atgaataatt taaataaatt cgatacaggt catgccacaa tcgttgagac attaatttgg
     2521 ggtagcttgc tggcagcaac cttaaaacgc tggctaatca acgcggcaca aaagaagtat
     2581 aaccgggtgt tgtcgctttt taccggagca aaaactgccc atatgtggtg gataccattt
     2641 tgctttgata tagccatgag tcgtggtacg gatttagcaa aagccatgaa taacgcgctg
     2701 gcatttttag cgcaacattg ccaacggcaa aatgttaaac gagaccaaat aagtggcgta
     2761 tataagctgc ttaataacac aaataaatca ttgggttagg tcgttaaggt catacgtatg
     2821 aacgttatgt ccggcttttt tagccgcagt ttcaattttc ttttgcgcaa tataggtatg
     2881 tgcaatcccc accgtgcagg cggtaacgcc gacaatattc ataatgtttc tccgttaatt
     2941 aatttaataa attactaatc gatattagca agttggtatt aagcatcggc gttttctgtt
     3001 tctgaattga aaagcgcaat gattttatgt ggatcagatt caacaagtaa tttagcgata
     3061 atgtcttcat ccgccagtga cccagacact tgtgccaaca ggcgaatatg ggtggcattc
     3121 tgatccacca ggcaaaccgc aaataaaata acacagcgga catcgctgcc atccatggtc
     3181 tcccagggaa tggcatgttg cgtgcgtccg atggctaatg cggtacggga aacaccggaa
     3241 gatttaccat gtggaatagc gatatggttt tcaaagccgg tagagcctag ctcctcgcgt
     3301 aaccagacat cacgaataaa gtcttcctta ttggtgacgg cgccatcctg gataagtaaa
     3361 tcggtgagtt cttctatcac ctcttctttg gttctggcgg tcatatttaa cttgatgttt
     3421 ttaacattaa gcactgtcga aatatccatg attcacccgc attcatatag agtggtcgtt
     3481 aaataacaaa ccagcatcgc tggcgtgatg cagatctttt aaggcttatt caaatacaca
     3541 ctgtggttcg aagagaaata acccgttatg cagcgacagt ttcccgttgg aagatctcga
     3601 tcattctgtc ccacgcctgg ctgacatcct catggagcga aaacagaccc gatgtgccga
     3661 cgataaagac atctaccccg gattcagcaa tctgaccgaa ggttttctca ttgcaagagc
     3721 catcaatttc cgtcagatag ttatagccat tatttttgcg catgttgatg agctttctaa
     3781 tcttgttcag cgactcagga ataaactttt gtccggcata gccagggtca accgacatga
     3841 ccgtcacttt atccagcaga tgcgcatatt cggcaatgct ttctgccggt gtcgccgggt
     3901 tcagcaccac cccgcacttt ttgcccgcat ccttgatctg attcagcagg cggaatattt
     3961 tgttattcgc cgtctcggga tgaaagctga tgatatcggc gccggcctcg agacacatgg
     4021 gaatgatatc ttccggatga ttcaccatca gatgaacatc aatgggcaca gtagtaattt
     4081 ttttcagatt ctcaataaaa aagggagaca gtgtgatatt gcggacgtaa ttgccatcca
     4141 ttatatcaac atggtaaaaa tccgcttttt tattcattgc cgtgatctgc tctttgaact
     4201 gcgttaaatc catgcacatc agtgacggtg aaaatttagc gctcataaac cacctccgat
     4261 aaatagttag ttaaacattg cgatgatatg acccagcacg atgccgacaa cgccaaagtc
     4321 tgcgtctcca aaggttgtgg agcccagtcc catatccccc atcatgggca agagaatggc
     4381 aggcaggaac gaaatgagca gaccgtgagc aaaagcccct accaccgccc cgcgacgtcc
     4441 gccggtaatg ttgccgtaaa cccccgctgt ggccccgcag aaaaagtggg gaaccaggcc
     4501 cgggacgatg acgctcaagc cgaacagcgg gcagaggaac atactgacca gccctgcgac
     4561 aaagcttgcg aggaagccga cgatgacggc attcggagcg aagggaaaca ccgtcggaca
     4621 gtccagcgcc ggcttcgcgt ctttaaccag tttgtccgca atccctttaa acgccgggac
     4681 gatctcggca atcaccatcc gaaccccggc gaggatgata taaactcctg cagcaaacgt
     4741 gagggactga ataatggcga agataataaa gttctgacca gcgctcaggg tctcctcgac
     4801 aaaggatttg cccgcaacga gcaccagcac cagaaacaga atcatcatgg tgagagagat
     4861 ggctaccgat gagtcgcgaa ggaaattgag cgacttaggt actttgatct cttcaatcga
     4921 gggactgcct ttaccaataa ttttgcccac cagcgcggac agcaggtagc caatagatcc
     4981 aaaatgaccg agcgccagat cgtcggtgtt ggttatttta cgggtaaacg gctgcaagat
     5041 agccggggag atgaccatca tcgcaccgag aataaccgcc ccgatggcca ccacccatcc
     5101 ccctgacaag ccccctacag acaggataac cgccagcatc gcagccatat aaagcgtatg
     5161 atgtccggtc agaaagatgt actttaatgg cgttagacgc gccagggcga tattgatcag
     5221 catgcccaat accataatgg tggcggtttc cgtgccgtaa ttcttctgcg ccagagcggc
     5281 catggcatca gtgttgggca ccacgccctg aatatggaaa gaatgttcaa ataactggct
     5341 aaagatggtt aacgtatagg ctataatacc ggcgccggcg gtcaatatga caaaacccat
     5401 gatggttttc agcgtgcctg aaataacgtc tgaaattcct tttttttgca acagcagacc
     5461 gatcagtgaa aataaaccaa ccagtatcgc cggtgttcct agcacatcat aaataagaaa
     5521 atgaagcatg ttaaactcca cgaatcagat agccgttgct taaatgtttt ctgctaaaat
     5581 ttctttaatt ttattgttat caagtaaatt acttaatcca acgacctttg cagagtgact
     5641 gctaagatgt tctgcaatgt ctttggcgct gagaataaga tcggcagttt cgctttttgc
     5701 cgacgtcagg tcggtatgtg cgacttcagc ttctttattc atttcggcaa ggatcttttt
     5761 gacattcatt tcaacgatga agctgctgcc caaaccatta ccgcacacga taagcacttt
     5821 tttcattctg ttttccttca gtcaataacg gcctgaatcg ccttgctatc atcggcattg
     5881 aacaatcggt gtaacttttg ctgatcgcta aagatttcag ccagttcacc aatcatcgtg
     5941 atatgttcat ttccggaacg agcgcatagt atgattacca cgtataccgg atcgttctct
     6001 gtcgagccga acgacacccc ttctttaata tgaagaagcg ataatccatt tcttaatacc
     6061 ccctgttcag gacgggcatg aggcatcgcc aagccgggag ctaatacgta atagggccca
     6121 agttctttat ggctttgcaa aatcgcctca atatacgacc cggctatata cccttgcatc
     6181 agcaagggtt ctgctgcaag tcgaacggca ttttcccagc tatctattga gttaatgact
     6241 ttaattttat ccggggttaa ccagtctctg atcatatgct ctcccgaggg ggtctatgtg
     6301 ctgcactata aacagctaaa taaaatgcaa ctgtgataca cctcccaaag ttagcgctaa
     6361 caaagaaaaa tgttaaaaaa tgcgttaccg ctaacactgg ctataatgtg ctttccccct
     6421 cgctgtggta agttataatg cgattcgcag atatactttc gttatatttt atttttcaaa
     6481 cggttggtga ttttggtgaa gagtttagac gcggacggta gcccgaaaga aaaacgacgt
     6541 aaaggaacgg gtaagataac cctgaccgag gtggctgagt ttgtcggtgt gagcgccatg
     6601 accgtttcgc gagcgctgag aatgcctgaa aaagtgaatc ctgagcttcg cgatcgcatc
     6661 gatgcggccg tcagtcaact cggctatgtc cctaatctgc aggcacgtaa tctggcgtcg
     6721 gtccattccg atcttattct cgccgtcgtc cccaccttct cttcaccggg ttttttgccg
     6781 gtctctgaag cgctgcaaaa agtgctgacc gaacgcggat ataccatgat gtttatggaa
     6841 tccgggcatg acggtcaaag tgaacaaaag acgtttgaaa aaatgctcgc ctataacccg
     6901 gcggccatta ttcagttcaa tatcgatacc attgagagct gcacccagct gctggccaac
     6961 gtggatgttc ctattctgga aattggcgcg ctaaataccc acccagtggg tatgtgcatc
     7021 ggcgtggatt acggtaaggc cgtaaaacag atcgtgactc atctggccga cgcctcatta
     7081 aaaaacatcg cactgctctg taccccggca aataacacaa tgtttcgcca gttgctgagc
     7141 ggatggaata cggcgatgct cgctctgaac cgctccccgc accgggtggt aaccacgcat
     7201 ttgccatcca ccatcgccac aggcgtcaat atattcaagg atatgatgat tacctggggc
     7261 gatctggatg cgttaatttg cacgtcggat gaaatggcct gtggatgtat gatggcctgc
     7321 cacagtgccg gcattaaggt gcccaatacc gtagccattg cgagtttagg cggaggagtg
     7381 ttatcgactg tctgttcccc tgccctgaca accgttgagt ttccctggca tgacattggg
     7441 gtgaaagccg gtaaagccct tctcgaactg cttaacgata agcctggtga aaagttcatt
     7501 gaaattccgt cagtgctcaa agtccgggcc agtaccgcgg catgagttta atatagggcg
     7561 ataaggcttc ccctatctag tatctgtgtg tggtagccag ggttttctac caggaagaat
     7621 gtctctctgc gagagtccat acaataaatg cccagctcat aaagcaaaaa atagtcggga
     7681 cagtagcgtt ttttcgctac taagcgggta aaccgccagc ctcatttccc gtttatcttg
     7741 ccagccgtcc caggcggtga tttcgtagcc tcgacccgcc tgggcggctt tgcactgaag
     7801 tccgtctagc gccagtccag gatatacgat gtctcctaag ccagttagca ggtaaagcgc
     7861 accgcgataa ttcatttgca ccgccctggt gccgcacatt caccatgccg ccccccgtca
     7921 gacatgccct cattacatta accacttgaa tataaaaaat tattttatat ggcacgttta
     7981 atgctcttcg cttgtatacc gcaatgtgga caagaacgta tgccatatgg ccacaacagg
     8041 tcgccagtct gcggtcgcaa ccttcccctt tctgtgaggc atccagggtg gatgcgtttc
     8101 acggaagcct gcttactgaa aacggagaac aacatgacga cactcagcga accgttggtt
     8161 accccgaaat atggcaaatg gcgccagctg g
//
