LOCUS       NZ_CP129745            11970 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2018C09-174 plasmid p2018C09-174-11k,
            complete sequence.
ACCESSION   NZ_CP129745
VERSION     NZ_CP129745.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281307
            Assembly: GCF_030489985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 21x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 21:10:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,818
            CDSs (total)                      :: 5,693
            Genes (coding)                    :: 5,556
            CDSs (with protein)               :: 5,556
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 52 of 137
            Pseudo Genes (incomplete)         :: 83 of 137
            Pseudo Genes (internal stop)      :: 11 of 137
            Pseudo Genes (multiple problems)  :: 9 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129745.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2018C09-174"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2018C09-174-11k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2018"
                     /collected_by="National Health Research Institutes"
     gene            complement(join(10193..11970,1..187))
                     /locus_tag="QYQ65_RS28115"
                     /old_locus_tag="QYQ65_28115"
     CDS             complement(join(10193..11970,1..187))
                     /locus_tag="QYQ65_RS28115"
                     /old_locus_tag="QYQ65_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(1671..1820)
                     /locus_tag="QYQ65_RS28120"
                     /old_locus_tag="QYQ65_28120"
     CDS             complement(1671..1820)
                     /locus_tag="QYQ65_RS28120"
                     /old_locus_tag="QYQ65_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1905..2162)
                     /locus_tag="QYQ65_RS28125"
                     /old_locus_tag="QYQ65_28125"
     CDS             complement(1905..2162)
                     /locus_tag="QYQ65_RS28125"
                     /old_locus_tag="QYQ65_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(2172..3857)
                     /locus_tag="QYQ65_RS28130"
                     /old_locus_tag="QYQ65_28130"
     CDS             complement(2172..3857)
                     /locus_tag="QYQ65_RS28130"
                     /old_locus_tag="QYQ65_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(3962..5449)
                     /gene="ltrA"
                     /locus_tag="QYQ65_RS28135"
                     /old_locus_tag="QYQ65_28135"
     CDS             complement(3962..5449)
                     /gene="ltrA"
                     /locus_tag="QYQ65_RS28135"
                     /old_locus_tag="QYQ65_28135"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            6094..6339
                     /locus_tag="QYQ65_RS28140"
                     /old_locus_tag="QYQ65_28140"
     CDS             6094..6339
                     /locus_tag="QYQ65_RS28140"
                     /old_locus_tag="QYQ65_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6613..6984
                     /gene="tolA"
                     /locus_tag="QYQ65_RS28145"
                     /old_locus_tag="QYQ65_28145"
     CDS             6613..6984
                     /gene="tolA"
                     /locus_tag="QYQ65_RS28145"
                     /old_locus_tag="QYQ65_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6981..7346
                     /locus_tag="QYQ65_RS28150"
                     /old_locus_tag="QYQ65_28150"
     CDS             6981..7346
                     /locus_tag="QYQ65_RS28150"
                     /old_locus_tag="QYQ65_28150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7521..7799
                     /locus_tag="QYQ65_RS28155"
                     /old_locus_tag="QYQ65_28155"
     CDS             7521..7799
                     /locus_tag="QYQ65_RS28155"
                     /old_locus_tag="QYQ65_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7787..8101
                     /locus_tag="QYQ65_RS28160"
                     /old_locus_tag="QYQ65_28160"
     CDS             7787..8101
                     /locus_tag="QYQ65_RS28160"
                     /old_locus_tag="QYQ65_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8265..8693
                     /locus_tag="QYQ65_RS28165"
                     /old_locus_tag="QYQ65_28165"
     CDS             8265..8693
                     /locus_tag="QYQ65_RS28165"
                     /old_locus_tag="QYQ65_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8719..8898
                     /locus_tag="QYQ65_RS28170"
                     /old_locus_tag="QYQ65_28170"
     CDS             8719..8898
                     /locus_tag="QYQ65_RS28170"
                     /old_locus_tag="QYQ65_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8925..9455)
                     /locus_tag="QYQ65_RS28175"
                     /old_locus_tag="QYQ65_28175"
     CDS             complement(8925..9455)
                     /locus_tag="QYQ65_RS28175"
                     /old_locus_tag="QYQ65_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9462..10193)
                     /gene="mobC"
                     /locus_tag="QYQ65_RS28180"
                     /old_locus_tag="QYQ65_28180"
     CDS             complement(9462..10193)
                     /gene="mobC"
                     /locus_tag="QYQ65_RS28180"
                     /old_locus_tag="QYQ65_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..8857
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          990..1568
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9466..10193)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tatggctcag ccccacaaaa cgcattcggc cggcccagag ttcagccaga ccgaaaaaca
       61 ggccgataac cagaaaaaaa ccggccagac tgtaatactc ccggtacagc cagctgaccc
      121 acgggtaaat ctgccgggcc agctccggga tatgccactg ccaggcaagc gaatccgtat
      181 taaacatgca atctctctcc ttcagacgac gggtttaccg gcgtgatgcg cagcccccgc
      241 tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg gtcacgtccg tggtgattgc
      301 gttcagcttt caccgcggag gccagcctct ttccgcccca tgcggaaaag gcgcagcccc
      361 ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc agtgcggtct
      421 tttccggtgt tccggccccc gtcagagttc gccatagcga gcggctgacc acccgcaaaa
      481 tcaggagcag ctttgctgct tcgtcaggtg cgtccagcac cgtggatttt tgtgggtggt
      541 caggcgtgag cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc cagccggaca
      601 gagccagaaa gaggggaaaa gaccgtgcgt cggtggcgtc cagccagcgt aagcaaggtc
      661 ttttcccctg aagtcagcca cgccccagcg gggatgactt gcatttctcc gcaaggagaa
      721 aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa aaaccgacat ggttcaccta
      781 tcctgataat tgatcgtcag ggaatagaaa gacttaatca ggggacaata tcccacatca
      841 gcgcgtccgg tgaccgtctt gcaggtgccg gggaaattgc gcctcctcgc tcactcggtc
      901 gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag
      961 attccgtgga taagcagggg actaacatgt gaggcaaaac ggcagggccg cgccggtggc
     1021 gtttttccat aggctccgcc ctcctgccag agttcacata aacagacgct ttttcggtgc
     1081 atctgtggga gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga
     1141 cttaaagatc cccaccgttt ccggtaggtc gctccctctt gcgctctcct gttccgaccc
     1201 tgccgtttac cggatacctg tccgccgttc tcccttgcgg gaagtgtggc gctttctcat
     1261 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc
     1321 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca
     1381 acccgggaaa gcacgcaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga
     1441 ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact
     1501 ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc
     1561 ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca
     1621 aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc
     1681 gcgatccccg tcagttcaga ggaggacgat ggtgccaccg ttccgccctg aacatcccgg
     1741 atataatttg cctgacatgc ggccagaaga aagccagcaa caatcaatat aaaaagaaaa
     1801 atagtcttta cctttttcat atactttccc tgtatttaaa gactccggca ttcatccctg
     1861 aacaccggaa gtcagtattt caaatcatcc ttgataattt tttgttacca gttaccgtcc
     1921 cgataatcaa aagctatata acaattatcc acatcaggtt ttatttgatt tttaaaaaat
     1981 ggctgtagta aaggaaccca ttctttcttc aaatcaaact caccattatt aatattatcc
     2041 tttaaaggca ttcccaagct ttcaatgaca gaaccatcgt caccaaagtc tttagaatac
     2101 tcttcccctt taaactcttc agttttctta tcaaaccatg tcaaacgtaa tttaagcccc
     2161 atatttacct ctcaaagata ttttttaatg ttccgttttg gatctggccc tttaacctgc
     2221 ttacccgttt tgggatcaaa tgcgcctagg tgttcgccat cactggcacg atacccttca
     2281 agctcaccgt gctgggaatc ccattcataa attttacgtt ttttatcgcc ataccatcgg
     2341 gcccgcttac caccaccacc ttgtttaggt gttttaggat cacctcgctt taactctcca
     2401 agtcccttta tgtcttctgt tttaggtact ggatgataat catggccgta gtctttagtc
     2461 cccttgcgag gcttgttctt ttcctcattt aacttatttt ctgcatcctt cgcttttttc
     2521 tccttttgtt tccggctctc catcgccccg gtaagtgcag ccttatctct ggataaggat
     2581 tcattcattg cgtttaaaga tgcctgagcc gcttttaatt tctcattctg attctgaata
     2641 tccttatcaa gatagcgaac ctgatcctgc cacccggagc catattcatg aggcgggtta
     2701 taagtcacaa aatcatcttt atttgcctct gcctctttta cctttttatc ggcttcctga
     2761 acagcagccc ttgcaccggg gagcgccttc tctgtcgatg caatgcggct gtttaaggtc
     2821 gcaatatttt tatcttcggc atccagctca gctttcgctt tatcatagtc tcttttcagc
     2881 ccctcttccg gatgagcggc gtcccatgcc tgctggcgac gcttttcttc ctcctgacgc
     2941 tgttttacct gtgccggggt aagaacatct gtcaccgaca cataaaccgg cttctgtccg
     3001 gtctctttcg ggaaacgaat aacggcctca cgggagttac caccggctgt aaaaccagcc
     3061 ggacgtgaat cacctttttc agcaacaatg ccttttggcg gggctttcgc tgtcggaaca
     3121 cctttcggta cgctcacctg cagagacggg agacccggaa tcgacacact gaataccccc
     3181 ggacgttttg tcggtttcgc atccaccaca gggacactca ttggccgacc tgcgacaacc
     3241 gcaatatgct gccgctcatc cttcaccaca tccacaaccc gttgtctgac acgaaccgtc
     3301 gcctggtcca gtggtaaagt acttgccgga gtctccgtca ccgcatcggc cggtaatgac
     3361 gtcataattt ttgacatcat tttcggatca tcttttgcaa tctcagaagg cagcacaccg
     3421 tagatcgcaa tcccccacag accaaactta aacggcccct tcagggcagc cagcacatca
     3481 gcaacggcgg ccgacaacgc atcgccggaa actgataaag ccagtccttc agcccccgga
     3541 gtagccaggg caggcagacc gaaggccatc gcagtggccg aagactgtcc gccaccagag
     3601 ctaccgctat gattgccgct gttaccacca ttaccaccgg gtttactccc gccgttacca
     3661 aactcccccg gattatagtt atgaatatct ccattcggcg tatgcgtcgt accccagccc
     3721 ggcccgctgt ttggatccgt accaccacct gatgaagggc cacctttacc agacgtaccg
     3781 ttcacattcc cactggcctg accaccatta tgtcccagac ctgaattacc cggacctcga
     3841 ccgtctccac cactcatacc gttttcctct ttgacattta aaacaataaa ttaaagccaa
     3901 catgtcgcgg tagggatacc cgttaccgga taccccccgc acagatcccg gcgtgcgcga
     3961 tttacgcacc gggctcctgc ctcgggtgtc tggcggtgaa ccgctccaca ggccatggat
     4021 gaagaacccg aacccttggt agccatgcgg ctgccagttt gtttgctttc gtccaggtcg
     4081 tatcatcctt ctggctcctg cgcctgagcg cccggcgcca gaggtttgtt acgtgtgtcc
     4141 tgaacttctg catggtgggg aagttgcccg gtaccgagtg atagttcagg tatccctgaa
     4201 ccactctcct gagccatttt ccctgttcgg ggattgagta atgccagcgc cttcgcagac
     4261 cgtctttgat ggctttcaga gttgccgtca tccgatcccg gcgggtcttt cgtatcagca
     4321 tgaacctgcc gttgcgatct ttcccgctga tgtgcgtgaa cccgaggaag ttgaacgttt
     4381 ctggtttgcc ttttcccctg atggcacggt tttcggcagc gaagcggccg aactccatca
     4441 gacgggtttt ctccgggtga accgtgagtc cgaactccct cagtctgcgc tgcatggcta
     4501 tacggaagcg ccgggcatcg tatcgtttgt cgaacccgat gacgatgtca tcggcgtatc
     4561 tgaccattac cacattgcct gtggcatagc gacgtcgcca ctgatgcgcc cacagatcga
     4621 agacgtagtg gaggtatatg tttgccagca gcggtgagat gaccgcaccc tgtggggtgc
     4681 cttcctccgt tgctcgccat tgaccctcct ccgacgtccc ggctgtgagc cacttacgta
     4741 tgagcctgat taccctccgg tcgccgatcc gatgctctgt gaacctgatc agccattcgt
     4801 ggctcaccct gtcgaagaac tgactgatgt cggcatccag tacccagttt acgttagtgc
     4861 gtaccagccc tgtggccagt gcgtccagtg catcgtgctg gcttcgcccg ggtctgaacc
     4921 cgtatgagaa ccccataaag tcgttttcat agactgcgtt caggattttc accagcgcat
     4981 actggacgat cttgtcctcc agcgaggcga tgccgagcgg gcgttgtttt ccatccgctt
     5041 ttgggatgta gtgacgcctg ccgggctgcg ccctgtagct gccctgatgt agcctccggt
     5101 gcagatctgt tatgttgttc ttcatgtttc cggcgtagtc catccacctg atgccatcca
     5161 ctccggcggc cgctttcctg ctcagggaga ggaatgcggc ttccagtgct tcgactgtca
     5221 gcaggtggaa caatgctgta aaccgttctt tcttccgctg cttcgcagct tcccgcacgc
     5281 gtgacagcct ctgtgacatg ctttcccggc tctgtgtccg gcgcatgtgt ggctgttccg
     5341 cgttcccctt ggccccgctc cttcgctcca ctgactccgc tcctttcggg ttgttcgcct
     5401 gcttcgccgc tactatgagc gagtccgact tctcctctcc gtacatcacc ggctatgact
     5461 cctcgtcttc ccggtgcggg ccatctccga cactggcaga tggtcagagg ggagatctcc
     5521 cggttcccgc gtagagatcg tattgacatg ccagggtctc agaccccgcc gggtccatgt
     5581 ggcactcgca gtatcgcacc ctatgatgtt gccttccgtt aacagtacaa cgtcggcacc
     5641 cggtaattta atatacattt cgtggctcaa tggctggcct gtcaacaccc ctgtcaacgc
     5701 ttcgccccat acctcgcggt atgcaacgca tgactcgggg accttgtgga ttgctggtcc
     5761 ttcaatggtc ggggactttc accccttgat ctctaaccgg tctcccggcg cacactgtac
     5821 atatacacag tataattatt accttactcc tcccgttttc aggtgtcaat atatgagatt
     5881 agttctcatc ttcaggcaca aaaaagcgcc cggagccacc tgtaagcaga tcgcgtcttt
     5941 tcccgaaaat ttctcatggc aacaaatttt attatttaag acattcatca cacttacatt
     6001 tcagatggta tagacacatc tgaaagctaa ctggcatgag gagaggacct gcggttaaac
     6061 tgttcgggaa acctgagacg cccggaggcc actatgacgc acctgaaact gactcccgct
     6121 gaagaaaagc gcctggcgga cgcctttgtc gctcaggcac tggccatggg cctgaaaggg
     6181 aacaaaacgc tgccaaaggc ggtaaaacaa caggtgcggg aaagggtgct tcgtgccatt
     6241 caggacgaac gtgaagccag gcgggcgcag gaaatgcgtc gggagaaata cgaggctacg
     6301 gaaaccacct tcacctggca gccacagcgc cgccggtaag gggtatttac cataacgtcg
     6361 cattgcccgc accgctcaga cggtctgagg gcgtttccgg cggctacggg ataaaacagg
     6421 caacaaacca cccggaaaac cgccggaaac gcgcccggta aattgtaacc cgctgcatgc
     6481 ctatgcacgc aactgcatgg cgggccgttt ggtgtaaaaa cgatacgata agccgtgttt
     6541 atgtaaatga acgtggccgt aaagagtgac aaatatacat gtaacatttt ttactgacag
     6601 gtcatcaacc cgatgcgttt aatacccttt atcctgctgg cattgtgcag ccccttactc
     6661 ctgacggcct gtaagcattc caccacccag ccgccgaaaa tttatactct ggataagtca
     6721 tctaaaatca gtagctacgc tgcggccatc aagaccgctg ttgaaatgca gatgtttgaa
     6781 ccagaggcct atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg tgatggcact
     6841 ctgaacagcg ccattgctga aggaggagat ccgaaactct gtaaagcagc aatatcagcc
     6901 gttacccacg ccaaaattcc gccggcaccg gatgaaaata cctggcaaat attcaaaaac
     6961 gcccctctgg atttcagacc atgaaagctt ttactctgac agtgctggcg gccatgttcc
     7021 tcactccggt acatgcgata ccactaaaac ccgtaaccac actgcagcca acctacccgg
     7081 tacaggccgc agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat attctggacg
     7141 atggacgagt ggacaacgtg tcgcttaccg gctgtcacct tttttttcgg gaaacgctga
     7201 cggcaatgag gcactggcta tatcccgcag ggcacccggc aatgcgacag gttataaatt
     7261 ttgaattcag tctgaaaaat gacggcttac aggatccgcg accagtcacc acccaaaagc
     7321 tcaatgatga tggttgccgc cagtaacagg tcctttaacc cgcaggctgc aatagtcctg
     7381 gatgatgagt gagcctgtag cagccctgat tagtggaagc gaagctgaaa aatgggcagc
     7441 aaaagccgta tttctggcaa atagcgccgc cacggattaa agtagtcatg tgtaatacat
     7501 atgactacaa aggggctttt atgtcatcag caacatccat caggctcgat gaagaactta
     7561 aggaccggct gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg
     7621 aggcaatcac cgaatacatc gaccgggaag agaagcgcag ccagtacctg cgggacggac
     7681 aggccgcatg gcagcactat caggaaacgg gtctgcacct tactgcggaa gaagcggaag
     7741 cctggataag tacctgggga actgagaacg agcaggatgc tccgccatgc cacaggtaac
     7801 gatttccgcg ctggcacagc gcgatttaca acgccttcag gactttctca aaaccaaaaa
     7861 tcggctggca gccagaaaag gaggtgaggt gatcgtccgg gctatccagc aactgaaaac
     7921 attgccagac attggccgcc cggtgccgtt tctgccgctg gaatataagg aactggtgat
     7981 cgggtttggc gacagtggct atgtgatgct ctaccgccac gaccgggaaa tggaccagat
     8041 tgtgatcgtc accgtcaggc atcagaaaga atccgggtat ccgggggcag acagcctcta
     8101 ataaccgatg acgggggcgc aattcattgc gtggccgtcg ccggtttgac aagcgcagcg
     8161 cgtcagaaaa ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg
     8221 agaaattaca cacctttcac acccgataaa atcaggggta tacagtggcg aaaacattgc
     8281 tcaacctttc acaggcagca caagccgcgg gcattaccag aagaacgcta tacaatcatg
     8341 taaaacaggg aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt
     8401 cagaactgat ccgtgtatac ggaaatgtga atatccctga gaaacaaatt cccggcattt
     8461 cacaccgtga aaatacacaa aaaaatttcc cacaggaaca gcttctggca atgcaaaagg
     8521 aactggccga tctccggcag gctgtcaccc tgatgctgga agataaaaca tcccgggaag
     8581 aagaacgccg ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg gacagattga
     8641 caacagaact gacacagaag aaaaaacgct tctggtccgg ctggtttagc tgagaactta
     8701 ccgaagggaa tggagaacat gacgaagcag gaaaaaactg ccctcaatat ggctaaattc
     8761 attaaggatc agtcgctgct gttgctggaa aagctgaacg agctggacat ggataccccg
     8821 gccgacatgt gcgaacggct tcacgaggat gcggaaacac tctggcaggt gatgcgggat
     8881 aagctcgggg aagaataaca gcaccggcca tgccggtgct ggcgttattt tttgccgaac
     8941 ggccaccacc ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac gttaaaatag
     9001 cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc gctttccccg
     9061 tgccgctcgc tgaccacctc aatcatcgcg ttaatctgct ccggtgagca cttcccggca
     9121 tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca cctttcgata gaggtaatca
     9181 cacagcttca ttcctgtggg tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag
     9241 tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga tttcatcatc
     9301 gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg ccaggcgagc
     9361 accaccatgt cttccagatg ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct
     9421 tccacgtcct caataaatac catgtacagc gccataatcc ctcactccgt tgtcataaat
     9481 ttaaagcacg cgaacagcct ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc
     9541 tgtggattca cgatttcatc caccgccaga atcgcccgct gtacccggac aagcgtcggc
     9601 cggtcagggc agatgtacca gatttcatcg atgccgttcg cccggcggtt gaacagatac
     9661 ccggacacca cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc
     9721 accgttctgc cgtccagctc cgccagcgcg tccggcactt tcatcccttt cagggccata
     9781 cggtgcaggt accgccagcc ggtggccccc cggccctcca tggagaggcg cagggactgc
     9841 acggccagcc ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa cggaataacg
     9901 tccgtcagcg gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac
     9961 acgctgacct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc tttcgccacc
    10021 agactgtcca ggaagcggta aaccggctgt accgccgcaa tccccagccg tcgcgcaacc
    10081 agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg tgttttctcc
    10141 gcgttccgcg cctgccgctt cgcgtggctg acgttgtact gctccagtgt catcagtaaa
    10201 gccccgcaaa atcatcatcc gcttttggtc cgccgccgtc ggccggaggc aggtactccg
    10261 ccagcgggtc cgttttcgcc agcaccggga aggttttcag cgaaatttcc cggcgctcaa
    10321 cccgcagcgg gctgctgaac gccagccgcg ccacgcccag cccggtcatc accgcgaacc
    10381 cgtcaggcag gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga
    10441 agctgtcgcc gctgatgtgc tccacgttcc ccgcctcccg gctggcccgg cggcgctcca
    10501 cgtccacccg gatctccccg gtctggcccg ccgcccactg cgcggactcc tggctggccg
    10561 cgcgatagaa ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca aaatcagcgg
    10621 gcagatcctg cccgcactga ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc
    10681 agtcccggct gcgcagcgtc cccagcgcgt tcagcacgta cttcgacagc agatatttaa
    10741 tctcatcgag catgatgctc gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga
    10801 tttgcgcgca gcgggcaaac agcatcttct gcacccggat caccgcctca tcgtccatcg
    10861 agccgatgac gtacaggcag ccgccgccgt tgattatccc cgccacatcg ataccgctgt
    10921 ccgtctgcag gacgctcagg tcggccacgt tctccagctt ggtgatcagc cccttcacgt
    10981 cctttttcag ggcctccggc aacgcgtacg ccgccgccag cacctgctga atgtttgccc
    11041 cctgcgggaa ctgctccgca atcagcttcg ccgctttctg ctcgctgatg cggtagtggt
    11101 ccgccacgtc gccggtctca ctcaggttaa acccggcaac cagcagattc ttcagggcgt
    11161 actggtcgag atcgcggaac agattcagct gcgccggttt ccccggccgc agatccagca
    11221 gggtgaagtc cgggcagtgc gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt
    11281 cgaagtacac cacagcgtcc ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca
    11341 tcgcgctctt gccgctcccg ggcaggccga ggatctgaat attggtcttg cgccaggtat
    11401 cacgagggac cagcaccggc tggccctgct catccttacc cagaaacacc gcctgctgtt
    11461 tccgggcctg ctccgtggaa aaatcggcca gcgcgttaac cggtaccggc tccccgaaat
    11521 gcaggtggcg gacgtctgtc atttctccgg tccgctggct gctgcgccgg ctctcctgat
    11581 tcagtttcgc gttcaccgcc tcgtcagcgg ccgcaaaatg accatcaagc agcaggaacg
    11641 ccagcagccg cgccagaagg acgccggcga caagccctcc ggcacaccag gctatcccga
    11701 acaggttagc cggctgcagc aggtaataat ctgcgttcgc atagaggtcc gggcgcagga
    11761 cggtttctcc ccagctctcg ataaacacgg cgacaaccag cccccccaga agggccagac
    11821 cggcagcgcc ggtcagccag tacgcgtagt tccagacatg tcgccgtgcc ctgaccatcc
    11881 gcccgggtga atccagcgag gccagcaccg aaaatggcac agtccggtta ttatcgcgct
    11941 tatagcgatc ggcatactcc cgccgcgcct
//
