LOCUS       NZ_CP129714            12556 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2018C06-172 plasmid p2018C06-172-12k,
            complete sequence.
ACCESSION   NZ_CP129714
VERSION     NZ_CP129714.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281297
            Assembly: GCF_030489925.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 24x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 20:44:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,732
            CDSs (total)                      :: 5,611
            Genes (coding)                    :: 5,472
            CDSs (with protein)               :: 5,472
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 47 of 139
            Pseudo Genes (incomplete)         :: 89 of 139
            Pseudo Genes (internal stop)      :: 14 of 139
            Pseudo Genes (multiple problems)  :: 11 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129714.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2018C06-172"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2018C06-172-12k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2018"
                     /collected_by="National Health Research Institutes"
     gene            complement(442..603)
                     /locus_tag="QYQ34_RS28590"
                     /old_locus_tag="QYQ34_28590"
     CDS             complement(442..603)
                     /locus_tag="QYQ34_RS28590"
                     /old_locus_tag="QYQ34_28590"
                     /inference="COORDINATES: protein motif:HMM:NF018252.2"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="WP_301699021.1"
                     /translation="MALPHSAIAEGGDPKLCKGSQHISNVTARQRIPPAPDENTWQIFK
                     NAPLDFRP"
                     /name="cell envelope integrity TolA C-terminal
                     domain-containing protein"
     gene            4298..5026
                     /locus_tag="QYQ34_RS28595"
                     /old_locus_tag="QYQ34_28595"
     CDS             4298..5026
                     /locus_tag="QYQ34_RS28595"
                     /old_locus_tag="QYQ34_28595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301699022.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGNG"
                     /name="hypothetical protein"
     gene            5023..6048
                     /locus_tag="QYQ34_RS28600"
                     /old_locus_tag="QYQ34_28600"
     CDS             5023..6048
                     /locus_tag="QYQ34_RS28600"
                     /old_locus_tag="QYQ34_28600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301699023.1"
                     /translation="MSRGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRCVRDFG
                     DAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNL
                     SETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLITKLENV
                     ADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRW
                     PHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVL
                     DNTPIRWFYRAASPGVRAVGGGPDRGDPGGRGAPPGQPGGGERGAHQRRQLHPEGGAAA
                     V"
                     /name="hypothetical protein"
     gene            5993..6265
                     /locus_tag="QYQ34_RS28605"
                     /old_locus_tag="QYQ34_28605"
     CDS             5993..6265
                     /locus_tag="QYQ34_RS28605"
                     /old_locus_tag="QYQ34_28605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228742589.1"
                     /translation="MEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGLGVARLAFSS
                     PLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="hypothetical protein"
     gene            6380..6748
                     /locus_tag="QYQ34_RS28610"
                     /old_locus_tag="QYQ34_28610"
     CDS             6380..6748
                     /locus_tag="QYQ34_RS28610"
                     /old_locus_tag="QYQ34_28610"
                     /inference="COORDINATES: protein motif:HMM:NF025186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_301699019.1"
                     /translation="MRDGWGIAAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTP
                     HGVAFSFDEDEPLTDVIPFQPSRVSAAQLPHRLAVQVPAPLHGGPGGHRLAVPAPYGPE
                     RDESAGRAGGAGRQNGGI"
                     /name="replication-relaxation family protein"
     gene            6738..6998
                     /locus_tag="QYQ34_RS28615"
                     /old_locus_tag="QYQ34_28615"
     CDS             6738..6998
                     /locus_tag="QYQ34_RS28615"
                     /old_locus_tag="QYQ34_28615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228733410.1"
                     /translation="MAFEVERTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLV
                     RVQRAILAVDEIVNPQTGEARKTVELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     gene            7005..7535
                     /locus_tag="QYQ34_RS28620"
                     /old_locus_tag="QYQ34_28620"
     CDS             7005..7535
                     /locus_tag="QYQ34_RS28620"
                     /old_locus_tag="QYQ34_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7562..7741)
                     /locus_tag="QYQ34_RS28625"
                     /old_locus_tag="QYQ34_28625"
     CDS             complement(7562..7741)
                     /locus_tag="QYQ34_RS28625"
                     /old_locus_tag="QYQ34_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7767..8195)
                     /locus_tag="QYQ34_RS28630"
                     /old_locus_tag="QYQ34_28630"
     CDS             complement(7767..8195)
                     /locus_tag="QYQ34_RS28630"
                     /old_locus_tag="QYQ34_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8359..8673)
                     /locus_tag="QYQ34_RS28635"
                     /old_locus_tag="QYQ34_28635"
     CDS             complement(8359..8673)
                     /locus_tag="QYQ34_RS28635"
                     /old_locus_tag="QYQ34_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8661..8939)
                     /locus_tag="QYQ34_RS28640"
                     /old_locus_tag="QYQ34_28640"
     CDS             complement(8661..8939)
                     /locus_tag="QYQ34_RS28640"
                     /old_locus_tag="QYQ34_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9114..9479)
                     /locus_tag="QYQ34_RS28645"
                     /old_locus_tag="QYQ34_28645"
     CDS             complement(9114..9479)
                     /locus_tag="QYQ34_RS28645"
                     /old_locus_tag="QYQ34_28645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(9476..9847)
                     /gene="tolA"
                     /locus_tag="QYQ34_RS28650"
                     /old_locus_tag="QYQ34_28650"
     CDS             complement(9476..9847)
                     /gene="tolA"
                     /locus_tag="QYQ34_RS28650"
                     /old_locus_tag="QYQ34_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(10121..10366)
                     /locus_tag="QYQ34_RS28655"
                     /old_locus_tag="QYQ34_28655"
     CDS             complement(10121..10366)
                     /locus_tag="QYQ34_RS28655"
                     /old_locus_tag="QYQ34_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11011..12498
                     /gene="ltrA"
                     /locus_tag="QYQ34_RS28660"
                     /old_locus_tag="QYQ34_28660"
     CDS             11011..12498
                     /gene="ltrA"
                     /locus_tag="QYQ34_RS28660"
                     /old_locus_tag="QYQ34_28660"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     Typing          6619..6995
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.683"
                     /coverage="100.0"
                     /evalue="1.7299999999999998e-69"
ORIGIN
        1 actattgcag cctgcgggtt aaaggacctg ttttaactgg caaagagcaa ccatcggtca
       61 ttgagctttt gggtggtgac tggtcgcgga tcctgtaagc cgtcattttt caggactgga
      121 attcaaaatg gttatcaggc ctgtgggcat gtgaacgggt gccctgcggg atatagccag
      181 tggcctcatt gccgtcagcg tggtggtccc gatccaaaaa gcggtgacgg gctggggtaa
      241 gcgacacgta cgtccgactc gtccatcgtc cagaactatc aatccgtggc ggtcacctga
      301 ccttcgatgc gttttgctgc ggcctgtaaa ccgggtaggt tggctgcagt gtggttacag
      361 cggcaatttt agtggtatcg catgtaccgg agtgaggaca gtcatcggcc actgccatgg
      421 cactgtcaga gtaaaagcct ttcatggtct gaaatccaga ggggcgtttt tgaatatttg
      481 ccaggtattt tcatccggtg ccggcggaat gcgttggcgt gccgtaacgt tgctgatatg
      541 ctggctgcct ttacagagtt tcggatctcc tccttcagca atggcgctgt gggggagtgc
      601 catctacgtt gtgctggata tccgcagatt gcaacagatt ttcccgcata ggcctctggt
      661 tcaaacattc tgcatttcat tacagcggtc ttgatggccg cagacgtaga ctactgcaag
      721 tactttacca tgactggatc cagagtataa attttcgtgt caggcaacgg gtggtggaat
      781 gcttacaggc cgtcaggagt aaggggctgc acaatgcggt tgtcagcagg ataaaggccc
      841 attaaacgtc atctgtggtt gatgacctga aacagtaaaa aatgttacgg gggtattcag
      901 tttgtcgact ctttacgggt ctcacgttca tttacaggta aacacggctt atcgtatcgt
      961 gttccgacag ccaaacggcc cgccaaaatg cattgttgcg tgcataggca tgcagcgtgg
     1021 ttacaattta ccgggctgcg ttttacagcc ggcggtaact tccgtgagtg ccgtttgttg
     1081 cctgttttat cccgaaagcc gaccggaaac gccctcagac cggtctgagc ggtgtcgggc
     1141 aatgcgacag ttatggtaaa taccccttac cggcggcgct gtggctaaaa cagcgtgaag
     1201 gtggtttccg aaaagcctcg tatttctccc gacgcatttc ctgcgcccgc ctggcttcac
     1261 gttcgtcctg aagaggcact gaaaagcacc ctatgataaa gcgaaacctg ttgttttacc
     1321 gcctttggca gcgtaaaaat attgttcgac cttaaacagg ccgcatggcc agtgcctgag
     1381 cagacaaagg cgctccgcca gtgcaagggc ttttcttcag cgggagtcag tttcaggtgc
     1441 gtcatagtgg cctccgagcg tctaaagatg atttcccgaa cagttataac cgcaggtcct
     1501 catgaatcat ggctccagtt agcaggatca gatgtcgtct ataccatctg ataatgtata
     1561 gtgtgatgaa tgtcttagaa tgagaattaa aatttgttgc tcatgagaaa tctttaaacg
     1621 ggaatgaatg acgcgatctg cttatccaga gataaggctc cgggcgcttt tttgtgcctg
     1681 aagatgagag ccggaatctc atatattgac acctgaaaag cgaaggatgg agtaaggtaa
     1741 taagttatac tgtgtatatg tacaagtgtg cgcaagggag accggttaga gatctaaggc
     1801 catgattagt catcccgtac cattgaagga ccagcaatcc ataaagggac ccgagtcatg
     1861 cgttgcatac cgcgaggtga tggggctaaa acacgttgac aaggtggtgg ttgtacaggc
     1921 cagccgattg tatggcgata cgaaatgtaa tattaaatta ccgggatgcc agcacgttgt
     1981 agctgttaac ggtatcgtgc aacagtcata gggtgcgaat acctaggcgc atttgatgcc
     2041 aacacgggta acccggttaa agggtctgag atccaaaact ggaacatgtc aatacgatct
     2101 ctgacgcggt aaatatggga gatctcccct ctgaccatct gccagtgtcg gagatggccc
     2161 gcaccgggaa gactgaggag ttcatagccg gaagatgtat tctaaagact ttggagaaga
     2221 tcggactctg ctcatagaaa gcttggcgaa tgcctttaag gcgatacata cccgaatagg
     2281 atgcgtgagt cagtggattt gaaggagcgg ggccaagggg aacgctttac tacagccatt
     2341 ttttacacat gcgccggaca ccagatgccg ggataagcat gtcacagagg ctgtcacgcg
     2401 tatcgggaag ctgcgtaagc agcggaagaa agaacggttt atcaagcatt gattccacct
     2461 gctgacagtc cgaagttcac tgatgaatgc cgcattcctc tccctgagca ggaaagcagg
     2521 ccgccggagt ggatggcatc aggtggatgg actatttttc cggaaacatt gattgttgct
     2581 ggcataacag atctggcacg catgtcaggc aaattacatc agggcatgct acagggcgca
     2641 gcccggcagg cgtcactaca tcctcaaaag cggatggaaa actgacgccc gctcggcatc
     2701 gcggttcagc tagaaaggat caaaggatcg tcttgagtat gctttttttc tgcgtgaaaa
     2761 tcttttgaac gcagtctatg aaaaaacgac ctggggatgg tgtttctcat acgaaggttc
     2821 agacccgttg gcgaagccag cttacgatgc acgtggtacg cactggcttt cacagggctg
     2881 gtacgcagct aacgtaaatc tgggtacttc cagtgatagc cggactttgg cagtcagttc
     2941 ttcgacaggg tgagccacga atggctgatc aggttcacag agcatcggat cggcgaactc
     3001 ggagggtaat caatggctgc atacgtaagt ggcgtcacag ccgggacttt cccggaggag
     3061 gactcaagtg aacgagttaa cggaggaagg caccccagtc agggctgaac ggggagttca
     3121 tgcttacagc cgctgcttgg agcgaacgac atataccgag ccgagatacc agtctgtcga
     3181 tctgtgagaa agcgccacac ttcccgcaag tggcgaacgt cgctatgcca caggcaatgt
     3241 ggtaaatggt caggatacgg aacgatgaca tcgtcaatcg ggttcgacca aacggatacg
     3301 atgcccggcg atcttccgta tagtccatgt cgggtttcgc cagtactgtc agggagttca
     3361 tggttgagcc tcacggttca ccacagatgc accgaaaacc cgtctgttta tgtgagttct
     3421 ggcaggaggg cgctgcctat ggaaaaccgt gccatcaggg gaaaaggcaa accagaaacg
     3481 ttttgcctca catgttagtc ctctgggtat ccacgcacat cagcgggtaa ctttgtatgt
     3541 gtccgcaacg cccgcagcag tctcatgccc tgatacgaag cgaccgagtg agcgaggagg
     3601 cgcaatttcc ccggcaactc tgaaagacca tcaccggacg cgctgatctg ggatattgtc
     3661 ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga accatgtcgg
     3721 tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
     3781 tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg cttacgctgg
     3841 ctggacgcca ccggacgcac ggtcttttcc cctctttctg gctctgtccg gctggacgcc
     3901 ggccacgcca gaaattcacc cggcccccgt caggctcacg cctgaccacc cacaaaaatc
     3961 cacggtgctg gacgcacctg acgaagcagc aaagctgctc ctgattttgc gggtggtcag
     4021 ccgctcgcta tggcgaactc tgacgggggc cggaacaccg gaaaagaccg cactggggtg
     4081 ccgccttttc ctctcacacg ctggggcgtg ctcgttgttc ctccggggct gcgccttttc
     4141 cgcatggggc ggaaagaggc tggcctccgc ggtgaaagct gaacgcaatc accacggacg
     4201 tgaccgccgc cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg gctgcgcatc
     4261 acgccggtaa acccgtcgtc tgaaggagag agattgcatg tttaatacgg attcgcttgc
     4321 ctggcagtgg catatcccgg agctggcccg gcagatttac ccgtgggtca gctggctgta
     4381 ccgggagtat tacagtctgg ccggtttttt tctggttatc ggcctgtttt tcggtctggc
     4441 tgaactctgg gccggccgaa tgcgttttgt ggggctgagc cataaggcgc ggcgggagta
     4501 tgccgatcgc tataagcgcg ataataaccg gactgtgcca ttttcggtgc tggcctcgct
     4561 ggattcaccc gggcggatgg tcagggcacg gcgacatgtc tggaactacg cgtactggct
     4621 gaccggcgct gccggtctgg cccttctggg ggggctggtt gtcgccgtgt ttatcgagag
     4681 ctggggagaa accgtcctgc gcccggacct ctatgcgaac gcagattatt acctgctgca
     4741 gccggctaac ctgttcggga tagcctggtg tgccggaggg cttgtcgccg gcgtccttct
     4801 ggcgcggctg ctggcgttcc tgctgcttga tggtcatttt gcggccgctg acgaggcggt
     4861 gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggaccggag aaatgacaga
     4921 cgtccgccac ctgcatttcg gggagccggt accggttaac gcgctggccg atttttccac
     4981 ggagcaggcc cggaaacagc aggcggtgtt tctgggtaac ggatgagcag gggccagccg
     5041 gtgctggtcc ctcgtgatac ctggcgcaag accaatattc agatcctcgg cctgcccggg
     5101 agcggcaaga gcgcgatggc caccaacgcg ctcattcgct gcgtacgcga tttcggggac
     5161 gctgtggtgt acttcgaccc caaaggggac gcgtgggcgc cgcacgtctt ccgggcgcac
     5221 tgcccggact tcaccctgct ggatctgcgg ccggggaaac cggcgcagct gaatctgttc
     5281 cgcgatctcg accagtacgc cctgaagaat ctgctggttg ccgggtttaa cctgagtgag
     5341 accggcgacg tggcggacca ctaccgcatc agcgagcaga aagcggcgaa gctgattgcg
     5401 gagcagttcc cgcagggggc aaacattcag caggtgctgg cggcggcgta cgcgttgccg
     5461 gaggccctga aaaaggacgt gaaggggctg atcaccaagc tggagaacgt ggccgacctg
     5521 agcgtcctgc agacggacag cggtatcgat gtggcgggga taatcaacgg cggcggctgc
     5581 ctgtacgtca tcggctcgat ggacgatgag gcggtgatcc gggtgcagaa gatgctgttt
     5641 gcccgctgcg cgcaaatcat cattgcgcgg gatgagttcc ggaggtggcc gcacgcgagc
     5701 atcatgctcg atgagattaa atatctgctg tcgaagtacg tgctgaacgc gctggggacg
     5761 ctgcgcagcc gggactgcaa cctgctgctc gcgcaccagt cgctgggcga cttcggtcag
     5821 tgcgggcagg atctgcccgc tgattttgtg aaaaccacgg tactggataa cacgccgatc
     5881 cgctggttct atcgcgcggc cagcccagga gtccgcgcag tgggcggcgg gccagaccgg
     5941 ggagatccgg gtggacgtgg agcgccgccg ggccagccgg gaggcgggga acgtggagca
     6001 catcagcggc gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct
     6061 gcaacacctg cctgacgggt tcgcggtgat gaccgggctg ggcgtggcgc ggctggcgtt
     6121 cagcagcccg ctgcgggttg agcgccggga aatttcgctg aaaaccttcc cggtgctggc
     6181 gaaaacggac ccgctggcgg agtacctgcc tccggccgac ggcggcggac caaaagcgga
     6241 tgatgatttt gcggggcttt actgatgaca ctggagcagt acaacgtcag ccacgcgaag
     6301 cggcaggcgc ggaacgcgga gaaaacacgg ctgaccctgc gctggctgcg ggaagagctg
     6361 tgttccacgg cggagctggt tgcgcgacgg ctgggggatt gcggcggtac agccggttta
     6421 ccgcttcctg gacagtctgg tggcgaaagg gctgctggtc cgggcgaaat acccggttga
     6481 cggccgtcag gtcagcgtgt gggggctgac gccgcacggg gtggcgttct cgttcgacga
     6541 ggacgagccg ctgacggacg ttattccgtt tcagccgtca agggtgtcgg cggcgcagct
     6601 gccgcaccgg ctggccgtgc aagtccctgc gcctctccat ggagggccgg ggggccaccg
     6661 gctggcggta cctgcaccgt atggccctga aagggatgaa agtgccggac gcgctggcgg
     6721 agctggacgg cagaacggtg gcatttgagg tagagcgcac cgtcaagagc cgcaggcggt
     6781 atcaggaagt ggtgtccggg tatctgttca accgccgggc gaacggcatc gatgaaatct
     6841 ggtacatctg ccctgaccgg ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg
     6901 atgaaatcgt gaatccacag accggcgagg cccgcaaaac ggtggagctg gaccgggaga
     6961 ggctgttcgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg ctgtacatgg
     7021 tatttattga ggacgtggaa gacaaagacg gcgagcgctg cattatgcca accagcgagc
     7081 atctggaaga catggtggtg ctcgcctggc agaccggggc gtatgttttc tgttttgtcg
     7141 accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag cggctcaaca
     7201 gcgacgaaaa agccacgtac tttttcaccg gtgaaaatcc gcaatgggaa cgggacaaac
     7261 ccacaggaat gaagctgtgt gattacctct atcgaaaggt ggagcggttc gccatcgaga
     7321 acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac gcgatgattg
     7381 aggtggtcag cgagcggcac ggggaaagcg cggtgaacga gaagctgaaa gcggagctga
     7441 tgcgggaata taacagccgc tattttaacg tgccggtgcc tgaactgccg gaggacaaac
     7501 ccgcccgccg gtggtggccg ttcggcaaaa aataacgcca gcaccggcat ggccggtgct
     7561 gttattcttc cccgagctta tcccgcatca cctgccagag tgtttccgca tcctcgtgaa
     7621 gccgttcgca catgtcggcc ggggtatcca tgtccagctc gttcagcttt tccagcaaca
     7681 gcagcgactg atccttaatg aatttagcca tattgagggc agttttttcc tgcttcgtca
     7741 tgttctccat tcccttcggt aagttctcag ctaaaccagc cggaccagaa gcgttttttc
     7801 ttctgtgtca gttctgttgt caatctgtcc acttcggcct ggagtttccg gcgctcatca
     7861 tcgtgctggc ggcgttcttc ttcccgggat gttttatctt ccagcatcag ggtgacagcc
     7921 tgccggagat cggccagttc cttttgcatt gccagaagct gttcctgtgg gaaatttttt
     7981 tgtgtatttt cacggtgtga aatgccggga atttgtttct cagggatatt cacatttccg
     8041 tatacacgga tcagttctga cacatccacg acagggttgt ttttcccgtc acgtgacacg
     8101 gtaacctttc cctgttttac atgattgtat agcgttcttc tggtaatgcc cgcggcttgt
     8161 gctgcctgtg aaaggttgag caatgttttc gccactgtat acccctgatt ttatcgggtg
     8221 tgaaaggtgt gtaatttctc acggtaaatg tatcgctgaa ttttacccgt ttttccgttc
     8281 aggatgtggt tttctgacgc gctgcgcttg tcaaaccggc gacggccacg caatgaattg
     8341 cgcccccgtc atcggttatt agaggctgtc tgcccccgga tacccggatt ctttctgatg
     8401 cctgacggtg acgatcacaa tctggtccat ttcccggtcg tggcggtaga gcatcacata
     8461 gccactgtcg ccaaacccga tcaccagttc cttatattcc agcggcagaa acggcaccgg
     8521 gcggccaatg tctggcaatg ttttcagttg ctggatagcc cggacgatca cctcacctcc
     8581 ttttctggct gccagccgat ttttggtttt gagaaagtcc tgaaggcgtt gtaaatcgcg
     8641 ctgtgccagc gcggaaatcg ttacctgtgg catggcggag catcctgctc gttctcagtt
     8701 ccccaggtac ttatccaggc ttccgcttct tccgcagtaa ggtgcagacc cgtttcctga
     8761 tagtgctgcc atgcggcctg tccgtcccgc aggtactggc tgcgcttctc ttcccggtcg
     8821 atgtattcgg tgattgcctc cagcattagc gcgtgcgctg agcggtgccg gtcatcggcc
     8881 agcgttttca gccggtcctt aagttcttca tcgagcctga tggatgttgc tgatgacata
     8941 aaagcccctt tgtagtcata tgtattacac atgactactt taatccgtgg cggcgctatt
     9001 tgccagaaat acggcttttg ctgcccattt ttcagcttcg cttccactaa tcagggctgc
     9061 tacaggctca ctcatcatcc aggactattg cagcctgcgg gttaaaggac ctgttactgg
     9121 cggcaaccat catcattgag cttttgggtg gtgactggtc gcggatcctg taagccgtca
     9181 tttttcagac tgaattcaaa atttataacc tgtcgcattg ccgggtgccc tgcgggatat
     9241 agccagtgcc tcattgccgt cagcgtttcc cgaaaaaaaa ggtgacagcc ggtaagcgac
     9301 acgttgtcca ctcgtccatc gtccagaata tcaaacgtgg cggtcacctg accttcgatg
     9361 cgttttgctg cggcctgtac cgggtaggtt ggctgcagtg tggttacggg ttttagtggt
     9421 atcgcatgta ccggagtgag gaacatggcc gccagcactg tcagagtaaa agctttcatg
     9481 gtctgaaatc cagaggggcg tttttgaata tttgccaggt attttcatcc ggtgccggcg
     9541 gaattttggc gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt
     9601 cagcaatggc gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt
     9661 ttcccgcata ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag
     9721 cgtagctact gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg
     9781 aatgcttaca ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta
     9841 aacgcatcgg gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg tcactcttta
     9901 cggccacgtt catttacata aacacggctt atcgtatcgt ttttacacca aacggcccgc
     9961 catgcagttg cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg
    10021 ttttccgggt ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt
    10081 ctgagcggtg cgggcaatgc gacgttatgg taaatacccc ttaccggcgg cgctgtggct
    10141 gccaggtgaa ggtggtttcc gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc
    10201 tggcttcacg ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt tgttttaccg
    10261 cctttggcag cgttttgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt
    10321 ccgccaggcg cttttcttca gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc
    10381 gtctcaggtt tcccgaacag tttaaccgca ggtcctctcc tcatgccagt tagctttcag
    10441 atgtgtctat accatctgaa atgtaagtgt gatgaatgtc ttaaataata aaatttgttg
    10501 ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt
    10561 gtgcctgaag atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta
    10621 ataattatac tgtgtatatg tacagtgtgc gccgggagac cggttagaga tcaaggggtg
    10681 aaagtccccg accattgaag gaccagcaat ccacaaggtc cccgagtcat gcgttgcata
    10741 ccgcgaggta tggggcgaag cgttgacagg ggtgttgaca ggccagccat tgagccacga
    10801 aatgtatatt aaattaccgg gtgccgacgt tgtactgtta acggaaggca acatcatagg
    10861 gtgcgatact gcgagtgcca catggacccg gcggggtctg agaccctggc atgtcaatac
    10921 gatctctacg cgggaaccgg gagatctccc ctctgaccat ctgccagtgt cggagatggc
    10981 ccgcaccggg aagacgagga gtcatagccg gtgatgtacg gagaggagaa gtcggactcg
    11041 ctcatagtag cggcgaagca ggcgaacaac ccgaaaggag cggagtcagt ggagcgaagg
    11101 agcggggcca aggggaacgc ggaacagcca cacatgcgcc ggacacagag ccgggaaagc
    11161 atgtcacaga ggctgtcacg cgtgcgggaa gctgcgaagc agcggaagaa agaacggttt
    11221 acagcattgt tccacctgct gacagtcgaa gcactggaag ccgcattcct ctccctgagc
    11281 aggaaagcgg ccgccggagt ggatggcatc aggtggatgg actacgccgg aaacatgaag
    11341 aacaacataa cagatctgca ccggaggcta catcagggca gctacagggc gcagcccggc
    11401 aggcgtcact acatcccaaa agcggatgga aaacaacgcc cgctcggcat cgcctcgctg
    11461 gaggacaaga tcgtccagta tgcgctggtg aaaatcctga acgcagtcta tgaaaacgac
    11521 tttatggggt tctcatacgg gttcagaccc gggcgaagcc agcacgatgc actggacgca
    11581 ctggccacag ggctggtacg cactaacgta aactgggtac tggatgccga catcagtcag
    11641 ttcttcgaca gggtgagcca cgaatggctg atcaggttca cagagcatcg gatcggcgac
    11701 cggagggtaa tcaggctcat acgtaagtgg ctcacagccg ggacgtcgga ggagggtcaa
    11761 tggcgagcaa cggaggaagg caccccacag ggtgcggtca tctcaccgct gctggcaaac
    11821 atatacctcc actacgtctt cgatctgtgg gcgcatcagt ggcgacgtcg ctatgccaca
    11881 ggcaatgtgg taatggtcag atacgccgat gacatcgtca tcgggttcga caaacgatac
    11941 gatgcccggc gcttccgtat agccatgcag cgcagactga gggagttcgg actcacggtt
    12001 cacccggaga aaacccgtct gatggagttc ggccgcttcg ctgccgaaaa ccgtgccatc
    12061 aggggaaaag gcaaaccaga aacgttcaac ttcctcgggt tcacgcacat cagcgggaaa
    12121 gatcgcaacg gcaggttcat gctgatacga aagacccgcc gggatcggat gacggcaact
    12181 ctgaaagcca tcaaagacgg tctgcgaagg cgctggcatt actcaatccc cgaacaggga
    12241 aaatggctca ggagagtggt tcagggatac ctgaactatc actcggtacc gggcaacttc
    12301 cccaccatgc agaagttcag gacacacgta acaaacctct ggcgccgggc gctcaggcgc
    12361 aggagccaga aggatgatac gacctggacg aaagcaaaca aactggcagc cgcatggcta
    12421 ccaagggttc gggttcttca tccatggcct gtggagcggt tcaccgccag acacccgagg
    12481 caggagcccg gtgcgtaaat cgcgcacgcc gggatctgtg cggggggtat ccggtaacgg
    12541 gtatccctac cgcgac
//
