LOCUS       NZ_CP129709            12157 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Klebsiella pneumoniae strain 2018C06-162 plasmid p2018C06-162-12k,
            complete sequence.
ACCESSION   NZ_CP129709
VERSION     NZ_CP129709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36281296
            Assembly: GCF_030489805.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Kuo,S.-C.
  TITLE     Klebsiella pneumoniae Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12157)
  AUTHORS   Kuo,S.-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) National Institute of Infectious Diseases
            and Vaccinology, National Health Research Institutes, 35 Keyan Road,
            Zhunan, Miaoli 35053, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTLink v. V11.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 25x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 20:56:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,731
            CDSs (total)                      :: 5,610
            Genes (coding)                    :: 5,467
            CDSs (with protein)               :: 5,467
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 51 of 143
            Pseudo Genes (incomplete)         :: 89 of 143
            Pseudo Genes (internal stop)      :: 14 of 143
            Pseudo Genes (multiple problems)  :: 11 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2018C06-162"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2018C06-162-12k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2018"
                     /collected_by="National Health Research Institutes"
     gene            539..2503
                     /locus_tag="QYQ39_RS28590"
                     /old_locus_tag="QYQ39_28590"
     CDS             539..2503
                     /locus_tag="QYQ39_RS28590"
                     /old_locus_tag="QYQ39_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2503..3234
                     /gene="mobC"
                     /locus_tag="QYQ39_RS28595"
                     /old_locus_tag="QYQ39_28595"
     CDS             2503..3234
                     /gene="mobC"
                     /locus_tag="QYQ39_RS28595"
                     /old_locus_tag="QYQ39_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3241..3771
                     /locus_tag="QYQ39_RS28600"
                     /old_locus_tag="QYQ39_28600"
     CDS             3241..3771
                     /locus_tag="QYQ39_RS28600"
                     /old_locus_tag="QYQ39_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3798..3977)
                     /locus_tag="QYQ39_RS28605"
                     /old_locus_tag="QYQ39_28605"
     CDS             complement(3798..3977)
                     /locus_tag="QYQ39_RS28605"
                     /old_locus_tag="QYQ39_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4003..4431)
                     /locus_tag="QYQ39_RS28610"
                     /old_locus_tag="QYQ39_28610"
     CDS             complement(4003..4431)
                     /locus_tag="QYQ39_RS28610"
                     /old_locus_tag="QYQ39_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4595..4909)
                     /locus_tag="QYQ39_RS28615"
                     /old_locus_tag="QYQ39_28615"
     CDS             complement(4595..4909)
                     /locus_tag="QYQ39_RS28615"
                     /old_locus_tag="QYQ39_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4897..5175)
                     /locus_tag="QYQ39_RS28620"
                     /old_locus_tag="QYQ39_28620"
     CDS             complement(4897..5175)
                     /locus_tag="QYQ39_RS28620"
                     /old_locus_tag="QYQ39_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5350..5715)
                     /locus_tag="QYQ39_RS28625"
                     /old_locus_tag="QYQ39_28625"
     CDS             complement(5350..5715)
                     /locus_tag="QYQ39_RS28625"
                     /old_locus_tag="QYQ39_28625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5712..6083)
                     /gene="tolA"
                     /locus_tag="QYQ39_RS28630"
                     /old_locus_tag="QYQ39_28630"
     CDS             complement(5712..6083)
                     /gene="tolA"
                     /locus_tag="QYQ39_RS28630"
                     /old_locus_tag="QYQ39_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6357..6602)
                     /locus_tag="QYQ39_RS28635"
                     /old_locus_tag="QYQ39_28635"
     CDS             complement(6357..6602)
                     /locus_tag="QYQ39_RS28635"
                     /old_locus_tag="QYQ39_28635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7247..8734
                     /gene="ltrA"
                     /locus_tag="QYQ39_RS28640"
                     /old_locus_tag="QYQ39_28640"
     CDS             7247..8734
                     /gene="ltrA"
                     /locus_tag="QYQ39_RS28640"
                     /old_locus_tag="QYQ39_28640"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            8839..10524
                     /locus_tag="QYQ39_RS28645"
                     /old_locus_tag="QYQ39_28645"
     CDS             8839..10524
                     /locus_tag="QYQ39_RS28645"
                     /old_locus_tag="QYQ39_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10534..10791
                     /locus_tag="QYQ39_RS28650"
                     /old_locus_tag="QYQ39_28650"
     CDS             10534..10791
                     /locus_tag="QYQ39_RS28650"
                     /old_locus_tag="QYQ39_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            10876..11025
                     /locus_tag="QYQ39_RS28655"
                     /old_locus_tag="QYQ39_28655"
     CDS             10876..11025
                     /locus_tag="QYQ39_RS28655"
                     /old_locus_tag="QYQ39_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             3839..12157
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11129..11707)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2504..3231
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cacccacaaa aatccacggt gctggacgca cctgacgaag cagcaaagct gctcctgatt
       61 ttgcgggtgg tcagccgctc gctatggcga actctgacgg gggccggaac accggaaaag
      121 accgcactgg ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac
      181 gcctgaccac ccacaaaaat ccacggtgct ggacgcacct gacgaagcag caaagctgct
      241 cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc
      301 ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt
      361 cctccggggc tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg cggtgaaagc
      421 tgaacgcaat caccacggac gtgaccgccg ccgccatgcc ttggggcatg tccgcggctt
      481 gtgcagcggg ggctgcgcat cacgccggta aacccgtcgt ctgaaggaga gagattgcat
      541 gtttaatacg gattcgcttg cctggcagtg gcatatcccg gagctggccc ggcagattta
      601 cccgtgggtc agctggctgt accgggagta ttacagtctg gccggttttt ttctggttat
      661 cggcctgttt ttcggtctgg ctgaactctg ggccggccga atgcgttttg tggggctgag
      721 ccataaggcg cggcgggagt atgccgatcg ctataagcgc gataataacc ggactgtgcc
      781 attttcggtg ctggcctcgc tggattcacc cgggcggatg gtcagggcac ggcgacatgt
      841 ctggaactac gcgtactggc tgaccggcgc tgccggtctg gcccttctgg gggggctggt
      901 tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgcccggacc tctatgcgaa
      961 cgcagattat tacctgctgc agccggctaa cctgttcggg atagcctggt gtgccggagg
     1021 gcttgtcgcc ggcgtccttc tggcgcggct gctggcgttc ctgctgcttg atggtcattt
     1081 tgcggccgct gacgaggcgg tgaacgcgaa actgaatcag gagagccggc gcagcagcca
     1141 gcggaccgga gaaatgacag acgtccgcca cctgcatttc ggggagccgg taccggttaa
     1201 cgcgctggcc gatttttcca cggagcaggc ccggaaacag caggcggtgt ttctgggtaa
     1261 ggatgagcag ggccagccgg tgctggtccc tcgtgatacc tggcgcaaga ccaatattca
     1321 gatcctcggc ctgcccggga gcggcaagag cgcgatggcc accaacgcgc tcattcgctg
     1381 cgtacgcgat ttcggggacg ctgtggtgta cttcgacccc aaaggggacg cgtgggcgcc
     1441 gcacgtcttc cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc
     1501 ggcgcagctg aatctgttcc gcgatctcga ccagtacgcc ctgaagaatc tgctggttgc
     1561 cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa
     1621 agcggcgaag ctgattgcgg agcagttccc gcagggggca aacattcagc aggtgctggc
     1681 ggcggcgtac gcgttgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct
     1741 ggagaacgtg gccgacctga gcgtcctgca gacggacagc ggtatcgatg tggcggggat
     1801 aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg
     1861 ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg atgagttccg
     1921 gaggtggccg cacgcgagca tcatgctcga tgagattaaa tatctgctgt cgaagtacgt
     1981 gctgaacgcg ctggggacgc tgcgcagccg ggactgcaac ctgctgctcg cgcaccagtc
     2041 gctgggcgac ttcggtcagt gcgggcagga tctgcccgct gattttgtga aaaccacggt
     2101 actggataac acgccgatcc gctggttcta tcgcgcggcc agccaggagt ccgcgcagtg
     2161 ggcggcgggc cagaccgggg agatccgggt ggacgtggag cgccgccggg ccagccggga
     2221 ggcggggaac gtggagcaca tcagcggcga cagcttcatc cagaaggagg cgcggccgct
     2281 gtttgacgtg aacaccctgc aacacctgcc tgacgggttc gcggtgatga ccgggctggg
     2341 cgtggcgcgg ctggcgttca gcagcccgct gcgggttgag cgccgggaaa tttcgctgaa
     2401 aaccttcccg gtgctggcga aaacggaccc gctggcggag tacctgcctc cggccgacgg
     2461 cggcggacca aaagcggatg atgattttgc ggggctttac tgatgacact ggagcagtac
     2521 aacgtcagcc acgcgaagcg gcaggcgcgg aacgcggaga aaacacggct gaccctgcgc
     2581 tggctgcggg aagagctgtg ttccacggcg gagctggttg cgcgacggct ggggattgcg
     2641 gcggtacagc cggtttaccg cttcctggac agtctggtgg cgaaagggct gctggtccgg
     2701 gcgaaatacc cggttgacgg ccgtcaggtc agcgtgtggg ggctgacgcc gcacggggtg
     2761 gcgttctcgt tcgacgagga cgagccgctg acggacgtta ttccgtttca gccgtcaagg
     2821 gtgtcggcgg cgcagctgcc gcaccggctg gccgtgcagt ccctgcgcct ctccatggag
     2881 ggccgggggg ccaccggctg gcggtacctg caccgtatgg ccctgaaagg gatgaaagtg
     2941 ccggacgcgc tggcggagct ggacggcaga acggtggcat ttgaggtaga gcgcaccgtc
     3001 aagagccgca ggcggtatca ggaagtggtg tccgggtatc tgttcaaccg ccgggcgaac
     3061 ggcatcgatg aaatctggta catctgccct gaccggccga cgcttgtccg ggtacagcgg
     3121 gcgattctgg cggtggatga aatcgtgaat ccacagaccg gcgaggcccg caaaacggtg
     3181 gagctggacc gggagaggct gttcgcgtgc tttaaattta tgacaacgga gtgagggatt
     3241 atggcgctgt acatggtatt tattgaggac gtggaagaca aagacggcga gcgctgcatt
     3301 atgccaacca gcgagcatct ggaagacatg gtggtgctcg cctggcagac cggggcgtat
     3361 gttttctgtt ttgtcgaccg ggcccagcat ctggcgatga tgaaatccat cgggatgtat
     3421 gacgagcggc tcaacagcga cgaaaaagcc acgtactttt tcaccggtga aaatccgcaa
     3481 tgggaacggg acaaacccac aggaatgaag ctgtgtgatt acctctatcg aaaggtggag
     3541 cggttcgcca tcgagaacgg tgtgcaggtg gttgatgccg ggaagtgctc accggagcag
     3601 attaacgcga tgattgaggt ggtcagcgag cggcacgggg aaagcgcggt gaacgagaag
     3661 ctgaaagcgg agctgatgcg ggaatataac agccgctatt ttaacgtgcc ggtgcctgaa
     3721 ctgccggagg acaaacccgc ccgccggtgg tggccgttcg gcaaaaaata acgccagcac
     3781 cggcatggcc ggtgctgtta ttcttccccg agcttatccc gcatcacctg ccagagtgtt
     3841 tccgcatcct cgtgaagccg ttcgcacatg tcggccgggg tatccatgtc cagctcgttc
     3901 agcttttcca gcaacagcag cgactgatcc ttaatgaatt tagccatatt gagggcagtt
     3961 ttttcctgct tcgtcatgtt ctccattccc ttcggtaagt tctcagctaa accagccgga
     4021 ccagaagcgt tttttcttct gtgtcagttc tgttgtcaat ctgtccactt cggcctggag
     4081 tttccggcgc tcatcatcgt gctggcggcg ttcttcttcc cgggatgttt tatcttccag
     4141 catcagggtg acagcctgcc ggagatcggc cagttccttt tgcattgcca gaagctgttc
     4201 ctgtgggaaa tttttttgtg tattttcacg gtgtgaaatg ccgggaattt gtttctcagg
     4261 gatattcaca tttccgtata cacggatcag ttctgacaca tccacgacag ggttgttttt
     4321 cccgtcacgt gacacggtaa cctttccctg ttttacatga ttgtatagcg ttcttctggt
     4381 aatgcccgcg gcttgtgctg cctgtgaaag gttgagcaat gttttcgcca ctgtataccc
     4441 ctgattttat cgggtgtgaa aggtgtgtaa tttctcacgg taaatgtatc gctgaatttt
     4501 acccgttttt ccgttcagga tgtggttttc tgacgcgctg cgcttgtcaa accggcgacg
     4561 gccacgcaat gaattgcgcc cccgtcatcg gttattagag gctgtctgcc cccggatacc
     4621 cggattcttt ctgatgcctg acggtgacga tcacaatctg gtccatttcc cggtcgtggc
     4681 ggtagagcat cacatagcca ctgtcgccaa acccgatcac cagttcctta tattccagcg
     4741 gcagaaacgg caccgggcgg ccaatgtctg gcaatgtttt cagttgctgg atagcccgga
     4801 cgatcacctc acctcctttt ctggctgcca gccgattttt ggttttgaga aagtcctgaa
     4861 ggcgttgtaa atcgcgctgt gccagcgcgg aaatcgttac ctgtggcatg gcggagcatc
     4921 ctgctcgttc tcagttcccc aggtacttat ccaggcttcc gcttcttccg cagtaaggtg
     4981 cagacccgtt tcctgatagt gctgccatgc ggcctgtccg tcccgcaggt actggctgcg
     5041 cttctcttcc cggtcgatgt attcggtgat tgcctccagc attagcgcgt gcgctgagcg
     5101 gtgccggtca tcggccagcg ttttcagccg gtccttaagt tcttcatcga gcctgatgga
     5161 tgttgctgat gacataaaag cccctttgta gtcatatgta ttacacatga ctactttaat
     5221 ccgtggcggc gctatttgcc agaaatacgg cttttgctgc ccatttttca gcttcgcttc
     5281 cactaatcag ggctgctaca ggctcactca tcatccagga ctattgcagc ctgcgggtta
     5341 aaggacctgt tactggcggc aaccatcatc attgagcttt tgggtggtga ctggtcgcgg
     5401 atcctgtaag ccgtcatttt tcagactgaa ttcaaaattt ataacctgtc gcattgccgg
     5461 gtgccctgcg ggatatagcc agtgcctcat tgccgtcagc gtttcccgaa aaaaaaggtg
     5521 acagccggta agcgacacgt tgtccactcg tccatcgtcc agaatatcaa acgtggcggt
     5581 cacctgacct tcgatgcgtt ttgctgcggc ctgtaccggg taggttggct gcagtgtggt
     5641 tacgggtttt agtggtatcg catgtaccgg agtgaggaac atggccgcca gcactgtcag
     5701 agtaaaagct ttcatggtct gaaatccaga ggggcgtttt tgaatatttg ccaggtattt
     5761 tcatccggtg ccggcggaat tttggcgtgg gtaacggctg atattgctgc tttacagagt
     5821 ttcggatctc ctccttcagc aatggcgctg ttcagagtgc catcacgttg tatggatatc
     5881 cgcacggtgc acatttttcc cgcataggcc tctggttcaa acatctgcat ttcaacagcg
     5941 gtcttgatgg ccgcagcgta gctactgatt ttagatgact tatccagagt ataaattttc
     6001 ggcggctggg tggtggaatg cttacaggcc gtcaggagta aggggctgca caatgccagc
     6061 aggataaagg gtattaaacg catcgggttg atgacctgtc agtaaaaaat gttacatgta
     6121 tatttgtcac tctttacggc cacgttcatt tacataaaca cggcttatcg tatcgttttt
     6181 acaccaaacg gcccgccatg cagttgcgtg cataggcatg cagcgggtta caatttaccg
     6241 ggcgcgtttc cggcggtttt ccgggtggtt tgttgcctgt tttatcccgt agccgccgga
     6301 aacgccctca gaccgtctga gcggtgcggg caatgcgacg ttatggtaaa taccccttac
     6361 cggcggcgct gtggctgcca ggtgaaggtg gtttccgtag cctcgtattt ctcccgacgc
     6421 atttcctgcg cccgcctggc ttcacgttcg tcctgaatgg cacgaagcac cctttcccgc
     6481 acctgttgtt ttaccgcctt tggcagcgtt ttgttccctt tcaggcccat ggccagtgcc
     6541 tgagcgacaa aggcgtccgc caggcgcttt tcttcagcgg gagtcagttt caggtgcgtc
     6601 atagtggcct ccgggcgtct caggtttccc gaacagttta accgcaggtc ctctcctcat
     6661 gccagttagc tttcagatgt gtctatacca tctgaaatgt aagtgtgatg aatgtcttaa
     6721 ataataaaat ttgttgccat gagaaatttt cgggaaaaga cgcgatctgc ttacaggtgg
     6781 ctccgggcgc ttttttgtgc ctgaagatga gaactaatct catatattga cacctgaaaa
     6841 cgggaggagt aaggtaataa ttatactgtg tatatgtaca gtgtgcgccg ggagaccggt
     6901 tagagatcaa ggggtgaaag tccccgacca ttgaaggacc agcaatccac aaggtccccg
     6961 agtcatgcgt tgcataccgc gaggtatggg gcgaagcgtt gacaggggtg ttgacaggcc
     7021 agccattgag ccacgaaatg tatattaaat taccgggtgc cgacgttgta ctgttaacgg
     7081 aaggcaacat catagggtgc gatactgcga gtgccacatg gacccggcgg ggtctgagac
     7141 cctggcatgt caatacgatc tctacgcggg aaccgggaga tctcccctct gaccatctgc
     7201 cagtgtcgga gatggcccgc accgggaaga cgaggagtca tagccggtga tgtacggaga
     7261 ggagaagtcg gactcgctca tagtagcggc gaagcaggcg aacaacccga aaggagcgga
     7321 gtcagtggag cgaaggagcg gggccaaggg gaacgcggaa cagccacaca tgcgccggac
     7381 acagagccgg gaaagcatgt cacagaggct gtcacgcgtg cgggaagctg cgaagcagcg
     7441 gaagaaagaa cggtttacag cattgttcca cctgctgaca gtcgaagcac tggaagccgc
     7501 attcctctcc ctgagcagga aagcggccgc cggagtggat ggcatcaggt ggatggacta
     7561 cgccggaaac atgaagaaca acataacaga tctgcaccgg aggctacatc agggcagcta
     7621 cagggcgcag cccggcaggc gtcactacat cccaaaagcg gatggaaaac aacgcccgct
     7681 cggcatcgcc tcgctggagg acaagatcgt ccagtatgcg ctggtgaaaa tcctgaacgc
     7741 agtctatgaa aacgacttta tggggttctc atacgggttc agacccgggc gaagccagca
     7801 cgatgcactg gacgcactgg ccacagggct ggtacgcact aacgtaaact gggtactgga
     7861 tgccgacatc agtcagttct tcgacagggt gagccacgaa tggctgatca ggttcacaga
     7921 gcatcggatc ggcgaccgga gggtaatcag gctcatacgt aagtggctca cagccgggac
     7981 gtcggaggag ggtcaatggc gagcaacgga ggaaggcacc ccacagggtg cggtcatctc
     8041 accgctgctg gcaaacatat acctccacta cgtcttcgat ctgtgggcgc atcagtggcg
     8101 acgtcgctat gccacaggca atgtggtaat ggtcagatac gccgatgaca tcgtcatcgg
     8161 gttcgacaaa cgatacgatg cccggcgctt ccgtatagcc atgcagcgca gactgaggga
     8221 gttcggactc acggttcacc cggagaaaac ccgtctgatg gagttcggcc gcttcgctgc
     8281 cgaaaaccgt gccatcaggg gaaaaggcaa accagaaacg ttcaacttcc tcgggttcac
     8341 gcacatcagc gggaaagatc gcaacggcag gttcatgctg atacgaaaga cccgccggga
     8401 tcggatgacg gcaactctga aagccatcaa agacggtctg cgaaggcgct ggcattactc
     8461 aatccccgaa cagggaaaat ggctcaggag agtggttcag ggatacctga actatcactc
     8521 ggtaccgggc aacttcccca ccatgcagaa gttcaggaca cacgtaacaa acctctggcg
     8581 ccgggcgctc aggcgcagga gccagaagga tgatacgacc tggacgaaag caaacaaact
     8641 ggcagccgca tggctaccaa gggttcgggt tcttcatcca tggcctgtgg agcggttcac
     8701 cgccagacac ccgaggcagg agcccggtgc gtaaatcgcg cacgccggga tctgtgcggg
     8761 gggtatccgg taacgggtat ccctaccgcg acatgttggc tttaatttat tgttttaaat
     8821 gtcaaagagg aaaacggtat gagtggtgga gacggtcgag gtccgggtaa ttcaggtctg
     8881 ggacataatg gtggtcaggc cagtgggaat gtgaacggta cgtctggtaa aggtggccct
     8941 tcatcaggtg gtggtacgga tccaaacagc gggccgggct ggggtacgac gcatacgccg
     9001 aatggagata ttcataacta taatccgggg gagtttggta acggcgggag taaacccggt
     9061 ggtaatggtg gtaacagcgg caatcatagc ggtagctctg gtggcggaca gtcttcggcc
     9121 actgcgatgg ccttcggtct gcctgccctg gctactccgg gggctgaagg actggcttta
     9181 tcagtttccg gcgatgcgtt gtcggccgcc gttgctgatg tgctggctgc cctgaagggg
     9241 ccgtttaagt ttggtctgtg ggggattgcg atctacggtg tgctgccttc tgagattgca
     9301 aaagatgatc cgaaaatgat gtcaaaaatt atgacgtcat taccggccga tgcggtgacg
     9361 gagactccgg caagtacttt accactggac caggcgacgg ttcgtgtcag acaacgggtt
     9421 gtggatgtgg tgaaggatga gcggcagcat attgcggttg tcgcaggtcg gccaatgagt
     9481 gtccctgtgg tggatgcgaa accgacaaaa cgtccggggg tattcagtgt gtcgattccg
     9541 ggtctcccgt ctctgcaggt gagcgtaccg aaaggtgttc cgacagcgaa agccccgcca
     9601 aaaggcattg ttgctgaaaa aggtgattca cgtccggctg gttttacagc cggtggtaac
     9661 tcccgtgagg ccgttattcg tttcccgaaa gagaccggac agaagccggt ttatgtgtcg
     9721 gtgacagatg ttcttacccc ggcacaggta aaacagcgtc aggaggaaga aaagcgtcgc
     9781 cagcaggcat gggacgccgc tcatccggaa gaggggctga aaagagacta tgataaagcg
     9841 aaagctgagc tggatgccga agataaaaat attgcgacct taaacagccg cattgcatcg
     9901 acagagaagg cgctccccgg tgcaagggct gctgttcagg aagccgataa aaaggtaaaa
     9961 gaggcagagg caaataaaga tgattttgtg acttataacc cgcctcatga atatggctcc
    10021 gggtggcagg atcaggttcg ctatcttgat aaggatattc agaatcagaa tgagaaatta
    10081 aaagcggctc aggcatcttt aaacgcaatg aatgaatcct tatccagaga taaggctgca
    10141 cttaccgggg cgatggagag ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat
    10201 aagttaaatg aggaaaagaa caagcctcgc aaggggacta aagactacgg ccatgattat
    10261 catccagtac ctaaaacaga agacataaag ggacttggag agttaaagcg aggtgatcct
    10321 aaaacaccta aacaaggtgg tggtggtaag cgggcccgat ggtatggcga taaaaaacgt
    10381 aaaatttatg aatgggattc ccagcacggt gagcttgaag ggtatcgtgc cagtgatggc
    10441 gaacacctag gcgcatttga tcccaaaacg ggtaagcagg ttaaagggcc agatccaaaa
    10501 cggaacatta aaaaatatct ttgagaggta aatatggggc ttaaattacg tttgacatgg
    10561 tttgataaga aaactgaaga gtttaaaggg gaagagtatt ctaaagactt tggtgacgat
    10621 ggttctgtca ttgaaagctt gggaatgcct ttaaaggata atattaataa tggtgagttt
    10681 gatttgaaga aagaatgggt tcctttacta cagccatttt ttaaaaatca aataaaacct
    10741 gatgtggata attgttatat agcttttgat tatcgggacg gtaactggta acaaaaaatt
    10801 atcaaggatg atttgaaata ctgacttccg gtgttcaggg atgaatgccg gagtctttaa
    10861 atacagggaa agtatatgaa aaaggtaaag actatttttc tttttatatt gattgttgct
    10921 ggctttcttc tggccgcatg tcaggcaaat tatatccggg atgttcaggg cggaacggtg
    10981 gcaccatcgt cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat
    11041 cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct
    11101 ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg
    11161 ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac
    11221 ttcaagagca accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg
    11281 ctgctgccag tggcgttttg cgtgctttcc cgggttggac tcaagtgaac agttaccgga
    11341 taaggcgcag cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac
    11401 gacctacacc gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca
    11461 agggagaacg gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag
    11521 ggagcgacct accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt
    11581 cagattcatg gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt
    11641 gaactctggc aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt
    11701 gcctcacatg ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg
    11761 cagcgcccgc cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat
    11821 ttccccggca cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt
    11881 aagtctttct attccctgac gatcaattat caggataggt gaaccatgtc ggtttttcgc
    11941 tgaacatttt acgttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc
    12001 atccccgctg gggcgtggct gacttcaggg gaaaagacct tgcttacgct ggctggacgc
    12061 caccgacgca cggtcttttc ccctctttct ggctctgtcc ggctggacgc cggccacgcc
    12121 agaaattcac ccggcccccg tcaggctcac gcctgac
//
