LOCUS       NZ_CP129618             5413 bp    DNA     circular CON 09-AUG-2023
DEFINITION  Klebsiella oxytoca strain NMI2990_17 plasmid p2, complete sequence.
ACCESSION   NZ_CP129618
VERSION     NZ_CP129618.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35742518
            Assembly: GCF_030343625.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Izdebski,R., Biedrzycka,M., Urbanowicz,P. and Gniadkowski,M.
  TITLE     Genomic epidemiology of VIM-producing Klebsiella oxytoca species
            complex in Poland, 2009-2019
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Izdebski,R., Biedrzycka,M., Urbanowicz,P. and Gniadkowski,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2023) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
REFERENCE   3  (bases 1 to 5413)
  AUTHORS   Izdebski,R., Biedrzycka,M., Urbanowicz,P. and Gniadkowski,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2023) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/04/2023 09:06:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,064
            CDSs (total)                      :: 5,944
            Genes (coding)                    :: 5,827
            CDSs (with protein)               :: 5,827
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 51 of 117
            Pseudo Genes (incomplete)         :: 76 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 31 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129618.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="NMI2990_17"
                     /isolation_source="clinical sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="p2"
                     /geo_loc_name="Poland:Bialystok"
                     /lat_lon="53.0807 N 23.0844 E"
                     /collection_date="2017-04-07"
                     /collected_by="National Medicines Institute"
                     /genotype="ST145"
     gene            complement(81..404)
                     /locus_tag="QTO83_RS30265"
                     /old_locus_tag="QTO83_030260"
     CDS             complement(81..404)
                     /locus_tag="QTO83_RS30265"
                     /old_locus_tag="QTO83_030260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1372..1698)
                     /locus_tag="QTO83_RS30270"
                     /old_locus_tag="QTO83_030265"
     CDS             complement(1372..1698)
                     /locus_tag="QTO83_RS30270"
                     /old_locus_tag="QTO83_030265"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_208644579.1"
                     /translation="MKLLDSNEVCELLGISKSTLYRWCGVSEDFSGLGLSSGLPQANAL
                     NSRLAKATQKSLGWENMLVGRALQQKALGVEETPSDFPRPFKIGRSFKWKDAEIMAWLE
                     TRRV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1971..2465)
                     /locus_tag="QTO83_RS30275"
                     /old_locus_tag="QTO83_030270"
     CDS             complement(1971..2465)
                     /locus_tag="QTO83_RS30275"
                     /old_locus_tag="QTO83_030270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000929317.1"
                     /translation="MAGTVWRDIRTGETVFPMGHLHPQRCCVDVNGTSVDIEISFGFHV
                     FTDEKQTGMLMKFKEEQRFFCRERYEGSKTIVHRILTAIENGEYITAFISKGQGQRYYH
                     LSHHDDFILMEIRKPQDRNNSLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            complement(2466..3047)
                     /locus_tag="QTO83_RS30280"
                     /old_locus_tag="QTO83_030275"
     CDS             complement(2466..3047)
                     /locus_tag="QTO83_RS30280"
                     /old_locus_tag="QTO83_030275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_208644578.1"
                     /translation="MNNSLILLVFFNKLVSIDKRETTMSARARKAGKRNQSERRQDLIE
                     KLWEDDEIDTWHRLENDGYSTVPRYLPLIGDLMDGLSKGSPLSTTYLALWFRVSDEGLI
                     EIRDKAALAFESGFASERGVTTWAGRMKKLKELGFISCREGSTGEFHYVLIVHPLVAVK
                     KLLDEGIIPKGKTYNILSERVIEVGASWEG"
                     /name="hypothetical protein"
     gene            complement(4103..4318)
                     /locus_tag="QTO83_RS30285"
                     /old_locus_tag="QTO83_030280"
     CDS             complement(4103..4318)
                     /locus_tag="QTO83_RS30285"
                     /old_locus_tag="QTO83_030280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4325..4816)
                     /locus_tag="QTO83_RS30290"
                     /old_locus_tag="QTO83_030285"
     CDS             complement(4325..4816)
                     /locus_tag="QTO83_RS30290"
                     /old_locus_tag="QTO83_030285"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<4851..5285)
                     /locus_tag="QTO83_RS30295"
                     /old_locus_tag="QTO83_030290"
                     /pseudo=""
     CDS             complement(<4851..5285)
                     /locus_tag="QTO83_RS30295"
                     /old_locus_tag="QTO83_030290"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(520..693)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          660..1165
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.095"
                     /coverage="99.0"
                     /evalue="1.71e-152"
     Typing          complement(88..404)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="1.59e-59"
     Typing          complement(4006..5411)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.451"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 tggcgccttc acgctgccgg tctttaccca gcagatagtc caccggcccg gcgccaccgc
       61 cgcgaccgcg tggatgaaat ttaacgatca tcgtccgttc ctttctcaag aacggcgtgc
      121 cgcagccgct caagcccggc atcgatggcc atcagcgccg ccacaatctg cacccggtca
      181 tgaccggtac cgacgccggc gttaacacgg cgggctatct ggttcaggtt gttgcccata
      241 ccggcaagct ggcgcagcag cgccggcgag atcgaaggaa gttttgcggt acgagccggc
      301 ttttcgtcca ggcaggtctg ccgcatccac gccgccagct gcctgccgtc gcagcgctca
      361 agtagccgcc tgtgctcatc ctccgtcacc cacatggtga gcatcttgtt ccgtttctct
      421 gacaaaacag tctcctgata accccgggac gggcgggaaa acctttcccg gtcggcgcgg
      481 ggttggacaa gccgcaggcg cgtcagtggc tgttagcggg tttcggggcg cagccctgaa
      541 ccagtcacgt agcgctagcg gagtgtatac tggcttacta tgttggcacg gatgcgaata
      601 tcagtgaagt gcttcatgtg gcgggagaaa aaaggcggca ccggtgcgtc agcagaatat
      661 gcgatacagg atatattccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      721 gcggcgagcg gaaatggctt acgaacgggg cggaaatttc ctggtagatg ccaggaagag
      781 acttaacagg gaaacgataa ggccgcggcg aagccgtttt tccataggct ccgcccccct
      841 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggacttaaa
      901 gataccaggc gtttccccct ggcggctccc tcttgcgctc tcctgttccc gtcctgcggc
      961 atccgtgttg tggtggaagc tttacctaaa tcaccacgtc ccgttccgtt tagacagttc
     1021 gctccaagct gggctgtgtg caagaacccc ccgttcagcc cgactaccac gttgtcccgg
     1081 taactatcat cttgagtcca acccggaaca gacacgaaaa atcgccagtg gcggtagcca
     1141 ttggtaaaca ggatgtgcga gagatgtatt cagagttctt gaagtggtgg ccttagtgag
     1201 gctacactga aaggacagat ttggtgacac gtctcgcaca agactgttac cacggttatt
     1261 caatctgcca ggattaaata accttcgaaa aaccacctgc cagggtggtt ttttcgttta
     1321 caggacaaaa gactacgacg agatccaaat gatctcaaga agatctatca atcaaacgcg
     1381 tcgcgtttca agccaagcca taatttcggc gtctttccat ttaaacgaac gaccgatctt
     1441 gaaaggacga gggaagtcgc ttggcgtctc ttcgacaccg agagcttttt gctgtaatgc
     1501 cctcccaacg agcatatttt cccagcctaa agatttctga gtagccttag ccagccggct
     1561 attcaaagcg ttagcctgag gaagaccgct acttagaccc aagccagaaa aatcctcaga
     1621 caccccgcac caacgataca aagtggactt actgatgcct aagagctcac acacttcatt
     1681 actatcaagc aacttcatat gaacacctat aaaaaacaat gaaaacctat aggcacttat
     1741 aaccccaaag atggtaaatg acaacctata aacatcaatg aacaccaaaa agtacgctag
     1801 tttgacaacg aacatgtgga ataggatact aatgttgtca gcacaaaccg gaacctcgag
     1861 agaggggtgc tgatgccggg ggtcacaccc tcccagcctg tgagcgggat aacgaaaggc
     1921 cagaccgaaa ttctgcaaaa aacaaaggcg ccttaggcgc tttttgtttt ttacacaatc
     1981 ttctttccct gcagtcgctg ttccagcaca tatctaaacc gcaggttacg tcctttattt
     2041 acagtccccc attcatcaag cgtgtaagct gtaacaacat gaatacgcaa agaattattt
     2101 ctatcttgag gctttcttat ttccatcaaa atgaagtcat catgatggct aagatggtaa
     2161 taccgctgcc cctgcccctt tgaaataaat gctgtaatgt attcaccgtt ttctatcgct
     2221 gtaagtattc tgtgaacgat agttttgctg ccctcatagc gttccctaca aaaaaacctc
     2281 tgttcctcct taaatttcat cagcattccg gtttgttttt catcagtaaa aacatgaaaa
     2341 ccaaaactaa tttctatatc tacagaagta ccgtttacat caacacagca cctctgaggg
     2401 tgcaggtgcc ccataggaaa aacagtctct cccgttctta tgtcacgcca tactgtgccc
     2461 gccatttaac cctcccaaga ggctcccacc tcaattacac gctccgacaa aatgttataa
     2521 gtctttcctt tagggataat cccttcatca agcagctttt tgactgcaac taatgggtga
     2581 acaattaaca cgtaatgaaa ttcaccggtg ctgccttcac gacaggatat gaaccccagt
     2641 tcttttagct ttttcattct ccctgcccat gtagttacgc ctctttcact agcgaaccct
     2701 gattcaaaag ccagtgctgc tttatctctt atttcaatca aaccctcatc agaaaccctg
     2761 aaccataaag ccaagtaggt tgttgataat ggtgatcctt ttgacagacc atccatgaga
     2821 tcgcctatca aaggcaaata acgtggaact gttgagtacc catcattttc aagtcgatgc
     2881 caagtatcga tttcgtcgtc ttcccacaat ttttcaatca aatcctgacg tcgttctgac
     2941 tgatttctct tacctgcttt tcttgctctt gcggacatag tcgtctcccg tttgtctatg
     3001 gacactaatt tattaaaaaa aaccaataaa atcaaagagt tattcatgac tggcagcttg
     3061 ttctcatctg aaaaaataga gttaaaaaca agctaaatcg caataatcat tacatttaaa
     3121 aacataaaaa gtaatatata ttacaatgaa gtttcttaat tatatgatta ataatgattt
     3181 acaagaggaa ggcggtactc gttgtgctcg gtgtgctcga tggttctccc tgtgctcctt
     3241 gtgctccatg gggttgtagg gggcaagcca gggcggatag agagaaaaat aatttagcgg
     3301 tagcgcgtct cagagactaa aaaacccgct gatgcgggtt ttcttattgt tacaaacatc
     3361 ctcgccgttg tagttcggct tcaatcgcct cacaagcagc gagtacaatc tcagtgttat
     3421 agcaccaggc tatgccactg cggttaatac tatcgattaa ggctcgatta tgagccaaag
     3481 tgcgttttag ggtggtggta tctttgtcca tcatcaggat ctccattcag gttaacgagc
     3541 ttcatgctcc cagatattta ctcttgccgt tcctgaacag gctaaaacag cgaaaaaaag
     3601 cctattttcg cttcatccca gagcaccaat atttaccata acatccgttg cccgcaccgc
     3661 tgaaacgggg ttggctgcgt ttccggtgcc agacgcgaga aagtggcgac agacatgacg
     3721 aaaaaacacc aaaaacgcag ccgctggata ttacactgct gcatagtcat gcagccagat
     3781 gcatggcgcg taaagtcaca ctaaaatcag cgcgaacgcc ttaaaaaccg gcaaatgcct
     3841 ctccagaaac tcacacagct ccatcaccag gtaaaaacag ttcagcaggg cttcaataac
     3901 gttgagaatt ttcatcactt cctccgggat aagtcactac cattctggta gtcagcctat
     3961 ttagcaggaa cgcgatgagc ccttcttcgt gcgggctgtc acctcatctt cggaccatcg
     4021 aaactcttct gcttctgtcg gagcgtcagc tccagctcgt tctgacgctg atactccagt
     4081 acagccttct cctcgatggc attcagttcc cggacaagcg gcgcagctgc tcgcttaaac
     4141 tcagcacctg cgtgctcaag tccgacacgc gctcgttcag caccgcgttc tcccgttgca
     4201 taagtccaca catgctccgc cattccgcga aggcgctctc ccattcgtcc agcctttgcg
     4261 agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg cttttccagc tctgtcattt
     4321 tccgctactc ctgtttcagg cggatccagg tcgtcccgtc gtaggggatg gcttcggctt
     4381 gcatccccgg cggcaggata aggaacctcc ggccgtcgtt gctctcctga tagcgcactc
     4441 cccacgtccg ggcggtcatt ttcgccaggg tatcttcctg ctgacgaaga tgcgtgtaat
     4501 tgtcggttat ctgccgtccc tgccaccaca gaatgctccc gctcgtggcg atcagcagcg
     4561 ctgacaccat caggatggtg agccatgtcc tcccggccgt cagcaacacg ctccgccggt
     4621 ttttctccat cgcctcgctc atactctgct cgtgggcgct gatggcgtcg ctgattctcc
     4681 tcgcgcttgc gttcagttcc gctctgacag actgctcgtg ctcgctgaat gcggctttca
     4741 gcatctcgcc ggtactcttc tgctgcgctt tcgattgctg ctctaagtcc ttcgccagcg
     4801 ttaaaagact gctcatagat ggctcccttc agtcggatgt ttcgcccccc gtcagggtcg
     4861 gcaatgctga tactgctttt cgttgtacgc accacctcaa aacctgcacc ttcaagagcc
     4921 tcagtgacgt cctgacggct tttcagctcc ccggacgagg ctaagctcag cagtccacgg
     4981 gtgatggctt ctgcggcttc ctgcttcgtt ttcggcagtc cggccggcgt gaccagcgcc
     5041 cggcggtttt ccggtgcgtt cgggtcgtgc agccccagtc tgccgttcac gatggtctgc
     5101 caggcatcga tgcgcggtcg gtcggcgcgg tcgtagtacg gctggagccg cctgccggtc
     5161 agtagctcgg tattggggat cagaaagttc agctccagac gccccttgtc cgcgtgctcc
     5221 acccacagaa tgctgtactg atctttatcg agtccgggca tcagaacccg ctcgaagctg
     5281 gccatcagct tttcccgctg gccgggaggc agatcggcct ccgcaaacga caggacgccg
     5341 gaggtgtatt tcttcacgta aggcgacgcg tcaatcagct cccggacttc ttccggcttc
     5401 ccctgcagga cgc
//
