LOCUS       NZ_CP129505             5820 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Enterobacter asburiae strain MO13m20 plasmid p5MO13m20, complete
            sequence.
ACCESSION   NZ_CP129505
VERSION     NZ_CP129505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35153056
            Assembly: GCF_030444535.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     VIM-1-producing Enterobacter asburiae with colistin resistance genes
            from wastewaters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2023) Center for Structural Biology, Vrije
            Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 13:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,355
            CDSs (total)                      :: 5,242
            Genes (coding)                    :: 5,146
            CDSs (with protein)               :: 5,146
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 20 of 96
            Pseudo Genes (multiple problems)  :: 20 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129505.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="MO13m20"
                     /isolation_source="water"
                     /db_xref="taxon:61645"
                     /plasmid="p5MO13m20"
                     /geo_loc_name="Czech Republic: Brno"
                     /lat_lon="49.130318 N 16.633070 E"
                     /collection_date="2016"
     gene            1..861
                     /locus_tag="QOM23_RS22985"
                     /old_locus_tag="QOM23_22985"
     CDS             1..861
                     /locus_tag="QOM23_RS22985"
                     /old_locus_tag="QOM23_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_301524524.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRIHAIGQHLKQPAREAIAEN
                     LTVQTIMGAMLNIMDERHRARVIEFCAGARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELVEVPNGKRG
                     SRDEL"
                     /name="helicase RepA family protein"
     gene            851..1744
                     /gene="repC"
                     /locus_tag="QOM23_RS22990"
                     /old_locus_tag="QOM23_22990"
     CDS             851..1744
                     /gene="repC"
                     /locus_tag="QOM23_RS22990"
                     /old_locus_tag="QOM23_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_301524525.1"
                     /translation="MNFELTHVRHDPAHCLAPGLFRSLKRGERKRLKLDVTYTHGDDSI
                     RFWGPEPLGVDDLRVLQGLVAMAAVSGEGGRGIVLSPEPKTGEGQQLRLWLDLKWDAIE
                     KDSMVAKGSFRQLARELGYADDGGSQFKTIRESIERLWAVSVIVERGGRRQGFRILSDY
                     ASDEHEGKLFVALNPRLAEAVMGERPHTRIDMGEVRALQTDPARLMHQRLCGWIDPGKS
                     GRVELDTLCGYAWPEQAKPEAMKKRRQTARKALAELAAVGWTVSEYAKGKWEVKRPAAP
                     TVTLPNPRRNSPQPPA"
                     /name="repC"
     gene            complement(2022..2480)
                     /locus_tag="QOM23_RS22995"
                     /old_locus_tag="QOM23_22995"
     CDS             complement(2022..2480)
                     /locus_tag="QOM23_RS22995"
                     /old_locus_tag="QOM23_22995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301524526.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSYDVYSYEPSDGLF
                     DSLTDEEKTELQSRIETERQEAEKRSRQYSAKSAASRIKEVADTIQGGDFEPSEAWAAD
                     TWAAIEALTKVMRKAGYPKPRKATQKAADAPMADQAGLPLGDAPESAS"
                     /name="hypothetical protein"
     gene            2936..3322
                     /locus_tag="QOM23_RS23000"
                     /old_locus_tag="QOM23_23000"
     CDS             2936..3322
                     /locus_tag="QOM23_RS23000"
                     /old_locus_tag="QOM23_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_301524520.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPEVNREAWAALARTAANLNQIAHRLNAGDALPLAAVRAT
                     LDAFRRDLIGVKHDAEDAEDESQG"
                     /name="ATP-binding protein"
     gene            complement(3513..3746)
                     /locus_tag="QOM23_RS23005"
                     /old_locus_tag="QOM23_23005"
     CDS             complement(3513..3746)
                     /locus_tag="QOM23_RS23005"
                     /old_locus_tag="QOM23_23005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301524521.1"
                     /translation="MLKRLGRLDGAAGFPLAKPHFPVQRYPAGHDVDVILVRVLVAHGH
                     PRRIGLVKTHSLHEVSRHGFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            4078..4761
                     /locus_tag="QOM23_RS23010"
                     /old_locus_tag="QOM23_23010"
     CDS             4078..4761
                     /locus_tag="QOM23_RS23010"
                     /old_locus_tag="QOM23_23010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301524522.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSVSLMHEDLKRLPGAVS
                     TETAQTLEPLARLRQDVTQVLEAYDKVTAIQRKTLDELTQQMSASAAQAFEQKAGKLDA
                     TISDLSRSLSGLKTSLSSMEQTAQQVATLPGDLASAQQGMTKAADQLTEAANETRPRLW
                     RRALGLILAGAVGAMLVATGQVVLDRLVPPSDVQQTANWGNAIWKNATPEERKLLEQIG
                     NRPAN"
                     /name="hypothetical protein"
     gene            4854..5747
                     /locus_tag="QOM23_RS23015"
                     /old_locus_tag="QOM23_23015"
     CDS             4854..5747
                     /locus_tag="QOM23_RS23015"
                     /old_locus_tag="QOM23_23015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301524523.1"
                     /translation="MTRQQDGRWLWCDRYGNDGGDNIDLLREIEPGTGYAEAVYRLSGA
                     PTVRQLPRPSEPKRQPPQLPAQGQAAREHGREYLKGRGITPDTIEHAEKAGMVRYADGG
                     VLFVGYDRRTGTAQNATRRAISPADPVQKRDLRGSDKGYPPILPGDPAKVWIVEGGPDA
                     LALHDIAKRSGQHPPTVIVSGGANVRSFLERADVQAILKRAERVTVAGENEKNPDAQTK
                     ADAGHQKQAQRVAEITSRPVRQWTPKPEQGKDLADMNARQVAEIERKRQAEIEAERARN
                     QELSLPEPGYDGPRFG"
                     /name="hypothetical protein"
     Typing          2..843
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.917"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3307..5744
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.882"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggctatca acattatgga ggcgttccag caggaaccgc cacaactgga tttcatctgg
       61 ccgggctttc tggctggcac cgtgggcgca ctggtggccc ccggcgcgac cggcaagagc
      121 ttctgggcgc tggaagcggc tatgtccatc gcttgcagcg tggcaggcgg tgacgtggtg
      181 aacctcaagc cgcagcacac cgggcgcgtg gtctatctgg ccggtgaaga tcccgaaccc
      241 gccctgatcc ggcgcattca cgccatcggc cagcacctga aacagccggc ccgagaagcc
      301 atcgccgaga atctgaccgt gcagaccatt atgggcgcga tgctcaacat catggacgaa
      361 cgccaccggg cgcgagtcat tgagttctgc gccggggcgc ggctgatcgt gctggacacc
      421 ctgagccgaa ttcacagcct cgacgagaac agcaacggcg acatggcgcg gcttgtcgcc
      481 acgctggagc acgtcgcggc cagcactggc gcgtctgtcc tgtacctgca ccacgtcagc
      541 aagggcagcg cccgcgaggg gcaaaccgac cagcagcagg cagcgcgggg cgcatcggca
      601 ctgatcgaca acgcccgctg gtgcggcttc gtcgccaaga tgaccgagga cgaggccaag
      661 cgcctgagcg accgcgccta tgaccgtcag cccatcggca acgaccggcg cggcttcttc
      721 gtgcgcttcg gcgtgagcaa acagaactac gacgccaccc cgcttgacca gtggtatgag
      781 cgccgagacg gcggcgtgct ggtgccggtg gaactggtgg aagtgccgaa cggcaagagg
      841 gggagccgcg atgaactttg aacttaccca cgtccggcac gatccggcgc attgtctggc
      901 ccccggcctg ttccgcagcc tcaagcgcgg cgagcgcaag cggctcaagc tggacgtgac
      961 ctacacccac ggcgacgact caatccgttt ctgggggcct gagccgctgg gcgttgatga
     1021 cctccgcgtg ttgcaaggat tggtggcaat ggccgctgta tcaggcgaag gcggacgcgg
     1081 cattgtgcta tcgcctgaac ccaaaaccgg ggaagggcag caattacgcc tatggctcga
     1141 tctgaaatgg gatgccatcg agaaagactc gatggtagcc aagggcagct ttcgccagtt
     1201 ggcccgagaa ctgggttatg ccgacgacgg cggcagccag tttaagacca tccgggaaag
     1261 catcgagcgt ctatgggctg tatcagtcat cgtggagcgc ggcgggcggc ggcaaggctt
     1321 ccgcatcctg tcggactacg cgagcgacga gcatgaaggc aagctgttcg tcgccctgaa
     1381 tccacggctg gccgaggccg tcatgggtga gcgcccccac actcgcatcg acatgggcga
     1441 agtgcgggcg ctacagaccg acccggcgcg gctgatgcac cagcggctgt gcggctggat
     1501 cgaccccggc aagtccgggc gggtagagct ggacacgctt tgcggctacg catggcccga
     1561 gcaagccaaa ccggaagcca tgaagaagcg gcgccagacg gcccgcaagg cactggccga
     1621 gcttgccgca gtgggctgga cggtgagcga gtacgccaag ggcaaatggg aggtcaagcg
     1681 gcccgccgcc cccacggtaa ctctccccaa cccccggcgt aactctcccc aacccccggc
     1741 gtaactctcc ccaacccccg gcgtaactct ccccgccccg atctggaaaa ccaagacggg
     1801 gcgcggcttt gcgggcgatt cgcaaaatcg atccaagatt ctccaagaat ctccactagg
     1861 cgcagcacag cccgcgcccc ttcggcgcgg tctggcattg cctcaaatgc ccggccaagg
     1921 ccggaaggcg aacacctctc cccccgcctt cggcgacccc ctccgggggt aaagggccag
     1981 cggggagagg gggtggaggc cggtttcccg gcctaaaccc atcaggaggc gctttctggc
     2041 gcatccccca agggcaaccc cgcctgatcc gccatcggcg cgtcagcggc cttctgtgtc
     2101 gctttgcggg gcttcggata gcccgctttg cgcatgactt tggtcagcgc ctcgattgcc
     2161 gcccaggtat ccgccgccca cgcctcggac ggctcgaaat cgcccccttg gatcgtatcg
     2221 gcaacctctt taatccgact ggcggcagat ttggcggaat actgtcggct tctcttttct
     2281 gcctcctgtc gttccgtttc aatgcgcgac tgtagttctg ttttttcttc gtctgtcaga
     2341 ctgtcaaata gtccgtctga cggctcgtat gaatatacgt cataagaccc catcatcttg
     2401 acgatggcgc ggcgcttttc cttgtcatac ccccggtagg cgagcacctg aacccggcga
     2461 ccttggcgct tgaactgcat gtggcttctc cgtttgactg tgtggcttaa ttttagtccg
     2521 actgtcgcac tgtgtcaagt gtttattgtc ggtttctttt ttagtccgac tgtcgcactt
     2581 aaataagttg cactgtcgta ctaaataaag cttgacagtc tgccgccgct tcgatattat
     2641 agcggttatg ttatcagttc gccccggcgc tttgcccacc cctctccccg ctggcctgct
     2701 accgcccttg ggcggtcggg ccaagagcgc agcgagtggg ccgggtgggt ggctgtttgg
     2761 tgcttgcatg gagatgtgct attatggtat ttgcgcggtg gtttggtagg gagcaaggtg
     2821 taacctgcgc ttcgcttggt tctcaccctc cggccttcgg cctacgcccc cgctcccttc
     2881 ggtcgggggg ttgctgcgcg tttcgcttcg cttcacttgc tggagggctt cgcccatgaa
     2941 cactgacgaa tccaagcgcc gccgccgtgg gcctgcgccg ctcgacgcca cggacaagcg
     3001 cgggcatacc gtttcggtgc gcctcaacga tgccgagctt gcgcggctgg attcgcagcg
     3061 tgacgcggtg cagatgcagc gcggtgagta cctacgcgcc gccgcccttc atcgcctgcc
     3121 gcccactatc ccggaagtga accgggaagc gtgggccgcg ttggcccgca cggcggccaa
     3181 cctgaaccag atcgcgcacc ggctgaacgc tggtgacgcg ctcccgctgg ccgcggtgcg
     3241 ggcaacgctc gacgccttca ggcgtgacct gataggcgtc aagcacgacg cagaggacgc
     3301 agaagatgaa agccaaggtt aacaggggtg gcggttttcg cggtgcgctt aactacgttt
     3361 ttgacgtggg caaggaagcc acgcacacga aaaacgcgga gcgagtcggc ggcaacatgg
     3421 ccgggaatga cccccgcgaa ctgtcgcggg agttctcagc cgtgcgccag ttgcgaccgg
     3481 acatcggcaa gcccgtctgg cattgctcgc tttcactgcc tcccggtgag cgcctgagcg
     3541 ccgagaagtg ggaagccgtg gcggctgact tcatgcagag aatgggtttt gaccaggcca
     3601 atacgccgtg ggtggccgtg cgccaccagg acacggacaa ggatcacatc cacatcgtgg
     3661 ccagccgggt agcgctggac gggaaagtgt ggcttggcca gtgggaagcc cgccgcgcca
     3721 tcgaggcgac ccaatcgctt gagcataccc acggcctgac cctgacgccg gggctgggcg
     3781 atgcacgggc cgagcgccgc aagctgaccg acaaggaaat caatatggcc gtaagaacgg
     3841 gcgacgaacc gccgcgccag cgtctgcaac ggctgctgga tgaggcagtg aaggacaagc
     3901 cgaccgcgcc agaactggcc gagcggctac aggccgcagg cgtaggcgtc tgggcaaacc
     3961 tcgccagcac cgggcgcatg aacggctttt ccttcgaggt ggccggagtg ccgttcaggg
     4021 gcagcgactt gggcaagggc tacacatggg cggggctaca gaaagcaggg gtgacttatg
     4081 acgaaattag agaccgtgcg ggccttgaac gattcaggcc cgcagttgca gatagtggag
     4141 agcgtcagga cgttgcagca gtccgtgagc ctgatgcacg aggacttgaa acgcctaccg
     4201 ggcgcagtct cgaccgagac ggcgcagacc ttggaaccgc tggcccgact ccggcaggac
     4261 gtgacgcagg ttctggaagc ctacgacaag gtcacggcca ttcagcgcaa gacgctggac
     4321 gaactgacgc agcagatgag cgcgagcgcg gcgcaggcgt tcgagcagaa ggccgggaag
     4381 ctggacgcga ccatctccga cctgtcgcgc agcctgtcag ggctgaaaac gagcctcagc
     4441 agcatggagc agaccgcgca gcaggtcgcg accttgccgg gcgatctggc gagcgcacag
     4501 cagggcatga cgaaagccgc cgaccaactg accgaggcag cgaacgagac gcgcccgcgc
     4561 ctttggcggc gggcgctggg gctgattctg gccggggccg tgggcgcgat gctggtagcg
     4621 actgggcaag tcgttttaga caggctagtg ccgccaagcg acgtgcagca gacggcaaac
     4681 tggggcaacg cgatttggaa aaatgcgacg cccgaggagc gcaagttgct ggaacagatc
     4741 ggcaatcggc ccgcgaacta gacccgaccg cctaccttga ggccagcggc tacaccgtga
     4801 agcgagaagg gcggcacctg tccgtcaggg ctggcgatga tgaggcgtac cgtgtgaccc
     4861 ggcagcagga cgggcgctgg ctctggtgcg accgctacgg caacgacggc ggggacaata
     4921 tcgacctgct gcgcgagatc gaacccggca ccggctacgc cgaggccgtc tatcggctct
     4981 caggtgcgcc gacagtccgg cagctaccgc gcccgagcga gccgaagcgc cagccgccgc
     5041 agctaccggc gcaagggcag gcagcccgcg agcatggccg cgaatacctc aagggcaggg
     5101 gcatcacccc ggacaccatc gagcacgccg aaaaggcggg catggtgcgc tatgcagacg
     5161 gcggcgtgct gttcgtcggc tacgaccgcc gtacaggcac cgcgcagaac gccacacgtc
     5221 gcgccatttc ccccgctgac ccggtgcaga agcgcgacct acgcggcagc gacaagggct
     5281 atccgccgat cctgccgggc gacccggcaa aggtatggat agtggaaggt ggcccggacg
     5341 cgctagccct gcacgacatc gccaagcgca gcggccagca cccgcccacc gttatcgtgt
     5401 caggcggggc gaacgtgcgc agcttcttgg agcgggccga cgtgcaagct atcctgaagc
     5461 gggccgagcg tgtcaccgtt gcaggcgaga acgagaagaa ccccgacgcg cagaccaagg
     5521 ccgacgccgg gcaccagaag caggcgcaac gggtggccga aatcacctcg cgcccggtgc
     5581 gccaatggac gcccaagcct gaacaaggca aggacttggc cgacatgaac gcccggcagg
     5641 tggccgagat cgagcgcaag cgacaggccg agatcgaggc tgagagggcg cgaaaccaag
     5701 agctttcact gccagagccg gggtatgatg gcccccgctt cggctgataa gagcgcctgt
     5761 cagtcggtct agaccaccga ccgacaggct gaccatcatc aacctgtaca ggaggccgat
//
