LOCUS       NZ_CP129504             5149 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Enterobacter asburiae strain MO13m20 plasmid p4MO13m20, complete
            sequence.
ACCESSION   NZ_CP129504
VERSION     NZ_CP129504.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35153056
            Assembly: GCF_030444535.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     VIM-1-producing Enterobacter asburiae with colistin resistance genes
            from wastewaters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2023) Center for Structural Biology, Vrije
            Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 13:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,355
            CDSs (total)                      :: 5,242
            Genes (coding)                    :: 5,146
            CDSs (with protein)               :: 5,146
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 20 of 96
            Pseudo Genes (multiple problems)  :: 20 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129504.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="MO13m20"
                     /isolation_source="water"
                     /db_xref="taxon:61645"
                     /plasmid="p4MO13m20"
                     /geo_loc_name="Czech Republic: Brno"
                     /lat_lon="49.130318 N 16.633070 E"
                     /collection_date="2016"
     gene            40..363
                     /locus_tag="QOM23_RS22950"
                     /old_locus_tag="QOM23_22950"
     CDS             40..363
                     /locus_tag="QOM23_RS22950"
                     /old_locus_tag="QOM23_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            353..>865
                     /locus_tag="QOM23_RS22955"
                     /old_locus_tag="QOM23_22955"
                     /pseudo=""
     CDS             353..>865
                     /locus_tag="QOM23_RS22955"
                     /old_locus_tag="QOM23_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1332..1823
                     /locus_tag="QOM23_RS22960"
                     /old_locus_tag="QOM23_22960"
     CDS             1332..1823
                     /locus_tag="QOM23_RS22960"
                     /old_locus_tag="QOM23_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136397997.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     NNYTHLRQQEDTLAKMTTRTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1830..2045
                     /locus_tag="QOM23_RS22965"
                     /old_locus_tag="QOM23_22965"
     CDS             1830..2045
                     /locus_tag="QOM23_RS22965"
                     /old_locus_tag="QOM23_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2517..3008
                     /locus_tag="QOM23_RS22970"
                     /old_locus_tag="QOM23_22970"
     CDS             2517..3008
                     /locus_tag="QOM23_RS22970"
                     /old_locus_tag="QOM23_22970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123921711.1"
                     /translation="MVNQPSVDKGSDNTHVGDVVCFLSTHCLMELFMNADEVTRILAQS
                     SQNDWIVDDETGAFTYKNDLALHIKRADYDDYNSFNEPWAVRHPDSNACSVEYTVHYGS
                     SFVDRKTLVSVDGHRATLPMPASATDLNVSSADVNFAKIVNIGDRVDEYIQRSGLTIKP
                     "
                     /name="hypothetical protein"
     gene            complement(3065..3493)
                     /locus_tag="QOM23_RS22975"
                     /old_locus_tag="QOM23_22975"
     CDS             complement(3065..3493)
                     /locus_tag="QOM23_RS22975"
                     /old_locus_tag="QOM23_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032634161.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="helix-turn-helix domain-containing protein"
     gene            3778..4206
                     /locus_tag="QOM23_RS22980"
                     /old_locus_tag="QOM23_22980"
     CDS             3778..4206
                     /locus_tag="QOM23_RS22980"
                     /old_locus_tag="QOM23_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634162.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          4903..5076
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.143"
                     /coverage="100.0"
                     /evalue="2.63e-66"
     Typing          complement(4434..4979)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.091"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          354..865
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.83"
                     /coverage="100.0"
                     /evalue="9.57e-97"
     Typing          1143..2137
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.617"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgttatcagg aggccggttt gtcagagaaa cggagcaaga tgctcaccat gtgggtgacg
       61 gaggacgagc accggcggct gctcgagcgc tgtgacggca ggcagctggc ggcgtggatg
      121 cggcagatct gcctggacga aaagccggca cgttccggaa agctcccctc actctcgccg
      181 gcgctgctgc gccagcttgc cggcatgggc aataacctga accagatagc ccgccgtgtt
      241 aacgccggcg gcggcaccgg ccatgacagg gtacagattg tggcggcgct gatggccatc
      301 gatgccggcc ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt
      361 taaatttcat ccgcgcggcc gcggcggtgg cgccgggccg gtggactatc tgctgggtaa
      421 agaccggcag cgtgaaggcg ccaccgtcct gcaggggaag ccggaagaag tccgggagct
      481 gattgacgcg tcgccttacg ttaagaaata cacctccggc gttctgtcgt ttgcggaggc
      541 cgatctgcct cccggccagc gggaaaaact gatggccagc ttcgagcggg ttctgatgcc
      601 cggactcgat aaagatcagt acagcattct gtgggtggag cacgcggaca aggggcgtct
      661 ggagctgaac tttctgatcc ccaataccga gctgctgacc ggcaggcggc tccagccgta
      721 ctacgaccgc gccgaccggc cgcgcatcga tgcctggcag accatcgtga acggcagact
      781 ggggctgcac gacccgaacg cgccggaaaa ccgccgggcg ctggtcacgc cgtccggact
      841 gccgaaaacg aagcaggggt ccgcttggaa aacggaaatt ttcctacgtt aagcgttttg
      901 agcttaacga gtgatttcgt tccattgagc gcgaacccct gaccgtaagg gatgtacgga
      961 acgatctgac tgtccgttaa cagttttgct tactttcgta catgttattg gcatggtgta
     1021 cgcaatcgtt cggtttcgga cacagaaacg ctgtagcccc gcgaaccgag tgatatccaa
     1081 tagccgattg gttaaaaagt agtcttagcg taggttattt tcccttttcc aagcggaccc
     1141 caagcaggag gccgcagaag ccatcacgcg cggactgctg acccttgctt cgtccggcga
     1201 gctgaaaacc cgtcaggacg tcactgaggc gctgggacag tcaggttttg aggtggtgcg
     1261 caccacgaaa agcagtatca gcattgccga cccggacggg gggcgaaaca tccgactgaa
     1321 gggagccatc tatgagcagt cttttaacgc tggcgaagga cttagagcag caatcgaaag
     1381 cgcagcagca gagtaccggc gagatgctga aagccgcatt cagcgagcac gagcagtctg
     1441 tcagagcgga actgagcgca agcgcgagga gaatcagcga cgccatcctc gcccacgagc
     1501 agagtatgag cgaggcgatg gagaaaaacc ggcggagcgt actgcggact gccgggcgga
     1561 catggctcac catcctgatg gtgtcagcgc tgctgatcgg cacgagcggg agcattctgt
     1621 ggtggcaggg gcagcagata accaacaatt acacgcatct tcgtcagcag gaagataccc
     1681 tggcgaaaat gaccacccgg acgtggggag tgcgctatca ggagagcagc gacggccgga
     1741 ggtttcttat cctgccgccg gggatgcaaa ccgaagccat cccctacgac gggacgacat
     1801 ggatccgcct gaaacaggag tagtggaaaa tgacagagct ggaaaagcag ttgctgagcg
     1861 cattagagca gctacagcag gactactcgc aaaggctgga cgaatgggag agcgccttcg
     1921 cggaatggcg gagcatgtgt gggcttatgc aacgggagaa cgcggtgctg aacgagcgcg
     1981 tgtcggactt gagcacgcag gtgctgagtt taagcgagca gctgcgccgc ttgtccggga
     2041 actgaatgcc atcgagctcc ggcacaggga gaatgagtat cagaaaacac tggagctgga
     2101 gcgccagccg cagaaaacct accgcgggcc gacactttaa accatccagg cagctgacaa
     2161 cccgcacgaa gaggggctct tacttctccc gctaaatagg ttcgtgatca aaaaggtcac
     2221 gccttattca ggagcgggaa atgaagctgt taaatcttat tgaatcattg cttaactgcc
     2281 tgtatctggc ggcggagctg tgggactggc tggagaagca tttgtcggtt tttaaggcat
     2341 tggcgcggat tttagtgtga ctttaacatc catgcatctg gctgcatgac tatgcatcag
     2401 tgtaatatcc agcggctgcg ttttcggtgt tttttcgtca tgtctgtcgc cactttctcg
     2461 cgtctggcac cggaaacgca gccaaccccg tttcagcggt gcgggcaaca gatgttatgg
     2521 taaatcaacc gtccgttgat aaaggttctg ataataccca tgtaggtgat gttgtttgct
     2581 ttttatctac acactgcctg atggagctat ttatgaatgc tgatgaagtt acacgaattc
     2641 ttgcgcaatc gagtcaaaat gattggattg tggatgatga gacaggggcg tttacttata
     2701 aaaatgattt agcactgcac attaagagag ctgattacga cgactacaac tcgtttaatg
     2761 agccttgggc tgttcgtcat cctgatagta atgcgtgttc agtcgagtac accgttcact
     2821 atggttcttc gtttgttgat agaaaaacct tggtttccgt cgatggtcat cgtgcaacct
     2881 tgcctatgcc tgcatccgca acggatctaa acgtgtcatc ggctgatgtg aatttcgcca
     2941 aaattgtaaa cattggcgat cgcgttgatg aatacattca acgttctggt cttacgatta
     3001 agccctaatt taacaataag cccggcctcc aaatgcgggc tgttcctgca tgtaaccagc
     3061 ataactaatc ggcttttttc cttactgtat atattggctt tttatgtcgc ggctttctta
     3121 cttgctcgga cacagaagga ccaattatgt ctaaataatc tacaagcttt gtaaggtcgt
     3181 atatgttgcg gccaccatat cggttgtcgc tttttgatgt cggtttttta tctaagagat
     3241 tggctttttc taggtcatta atagcccttt gcaccgtcct tactgacacc cccatccgta
     3301 tagccaggct ctccattgag gggtgcggcc actcctgtag attccaccag tgtactatga
     3361 gattcagcaa ggtattgaat gctacagggc tcaatttctt ctcattttgt ataaaaaaca
     3421 acacagtagg tactgcagtc catcctaatg acagacctcc agccttccac tttttttcta
     3481 ttgcgtaact cattgattat cctgtaaata ttttttttga gatatatata atataagtat
     3541 tcatatgaat agggtgacac cagcgccatt attacacaac tataaaaggc taatctgaga
     3601 acagaaaaac atttctatct gcattaaata cggagttagt gatatgaaaa ccaaacaaca
     3661 tcatgatctt gtaaaagata ctgaaatatt tggtgtatcc ttgtctctgt tgatttccag
     3721 agcgaatgag agatctgtta caatgccatc tggtctaagt agggagcaga gaagagcatg
     3781 ggcagcggat caagcacgca aaatccacaa ttgaatgttg tctctttctc tgagacattc
     3841 aatctttatt acacatcccg cttttctcaa aaaacactcg ataaaatcga tgacttcatc
     3901 gaacatttcg agaagtacaa tctttttggc gatgccttaa aaggccatcc ggcttggatt
     3961 ggcaaggtat cgccatcttg gaacgtgcct gacagttacg aaaacaaaga agctattgaa
     4021 gaacatgcca gggctaataa attgtggcat gcccatatag gcgatcccgt tttcatcgat
     4081 acatttcacg ggaagtacaa agtctctgat tgggtcatcc acttccaacg tctagcgccg
     4141 aaccatataa aactgttaga gcttgggtat catgacccta tgcaactccc ccaaaatttc
     4201 gaatgatctc ttgttcggat gaatgatccc ttgagattcg ttgaccctcg tcgtaatctc
     4261 ttgcggtgaa aacgaaaaaa ccacccggca aggtggcttt ttcgaaggtt cgcaagagtt
     4321 gacgctcttt tgaaccgcgg taactggctt ggaggagcac agtcaccaaa actgtccttt
     4381 cagtgtagcc ttatcgcacc cgaaacttca agactctcct cctctaaacc tgttaccagt
     4441 ggctgctgcc agtggcgctt tgtcgtgttt ttccggattg gactcaagtt gatagttacc
     4501 ggaaaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     4561 actgccttcc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     4621 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc caccaggggg
     4681 aaacgcctgg tatctttaag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
     4741 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc ggccttatcg
     4801 tttccctgtt aagtttcttc ctggcatcta ccaggaaatt tccgccccat tcgtaagccc
     4861 gcaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     4921 atatcctgta tcacatattc tgctgacgca ccggcgccgc cttttttctc ctgccacatg
     4981 aagcactttc ccgacaaccg cactcatgcc aacataataa gccattatac actccgctag
     5041 cgctacgtga ctggttcagg gctgcgcccc gaaacccgcc aacagcctga cgcgcctgcg
     5101 gcttgtccaa ccccgcgccg accgggaaag gctttcccgc ccgtcccgg
//
