LOCUS       NZ_CP129503             2546 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Enterobacter asburiae strain MO13m20 plasmid p3MO13m20, complete
            sequence.
ACCESSION   NZ_CP129503
VERSION     NZ_CP129503.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35153056
            Assembly: GCF_030444535.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     VIM-1-producing Enterobacter asburiae with colistin resistance genes
            from wastewaters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2023) Center for Structural Biology, Vrije
            Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 13:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,355
            CDSs (total)                      :: 5,242
            Genes (coding)                    :: 5,146
            CDSs (with protein)               :: 5,146
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 20 of 96
            Pseudo Genes (multiple problems)  :: 20 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129503.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="MO13m20"
                     /isolation_source="water"
                     /db_xref="taxon:61645"
                     /plasmid="p3MO13m20"
                     /geo_loc_name="Czech Republic: Brno"
                     /lat_lon="49.130318 N 16.633070 E"
                     /collection_date="2016"
     gene            complement(join(1425..2546,1..51))
                     /gene="mobV"
                     /locus_tag="QOM23_RS22940"
                     /old_locus_tag="QOM23_22940"
     CDS             complement(join(1425..2546,1..51))
                     /gene="mobV"
                     /locus_tag="QOM23_RS22940"
                     /old_locus_tag="QOM23_22940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_042193062.1"
                     /translation="MAYAILRVEKTKNTSIAGKNSHNMRLRKTHNADPNLKSQNRILIG
                     SGDLRTDINARFQATNVKARNSTSVICNELVLTASPEFFANSKNLEDWIKVQMEYLHNE
                     YGENAINAVLHLDEQTPHIHAFITPIENKNGIYKLNNKSYMKKYETMQDIYFKYNKPLG
                     LIRGIKKEVSNAEYKEVKEFYSDIKNIKNETELEIENNKIEKIRVVETEEKKKLFRDPE
                     VVPKHYTGAEVNRYIRKALKPYKNERKPLIARLNGFKSRLEHSEAELSDLRQNFNRRVQ
                     ERVQQEQKLAVARATAEQEQIIQNKNRKLKDMELEIIEYKRQLKENASISQRYEKYKIN
                     SDVLDLIQEHKPAEFKQLFNAAIAAADAEYSQKQQNNNYTPQNKPKALKL"
                     /name="mobV"
     gene            777..1376
                     /locus_tag="QOM23_RS22945"
                     /old_locus_tag="QOM23_22945"
     CDS             777..1376
                     /locus_tag="QOM23_RS22945"
                     /old_locus_tag="QOM23_22945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052685777.1"
                     /translation="MKKKNNIEYLQLNNAENSEDFDIEHKKNPFVFNMEIDTKKKKLTV
                     DRGSELETEEGVFTTTISQIQEVDKEEFLKLFTGQIKFYFDLTQTGFKLFFLIMGIYQK
                     QIGGDEIYLNFETAKEEAEKYDYTISKAVYYRGLKELLEKKMIAKSVKKYIFYINPAIV
                     FNGDRARFVKEIRIKEEEKQREADLKSINERLDRYA"
                     /name="hypothetical protein"
     Typing          complement(1426..1622)
                     /name="Col(IMGS31)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.85e-99"
     Typing          complement(1429..2546)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.66"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 agcgattgaa gtattttttg ttttttccac tcttaaaatt gcataagcca tattcgaaaa
       61 cctcccggta aaagcagtaa ggtttttttc tttttggccc ctgccaggct cacaccgaga
      121 tttctcggta tagtgagtat accttttctg caaaattgaa aatctataaa tacatctaca
      181 ataaaaaaaa caaaagtcaa cggatcaatc cctcgcaagg gaaaattaaa atttcccctt
      241 actcacgatt tccaataaaa gaaaaaagac agaacgctga gcaagtcaaa attttaattt
      301 tggcttgtga agggttgacc aagcgaagcg cggtagggaa atctgcgcag attgcttatg
      361 tattgcccgg aacgggaaac gtctgttgta gcggtagcga aaacacatct cccggaaggg
      421 ggttttcttt tgcgtagcct ggcaagttct gcagataggg ctgggactag gttgaatcct
      481 cttagatctt atatttctta tattcttaag gggtctgtaa cccccgtaaa acctaacggt
      541 ctagtctctc acatgtaaaa aaattctctc ctgagagaat ttattctctc ctgagttact
      601 aatagtctct cctgagttac tatttgtatc gttttttttt gaaaaaacga gaaaatattt
      661 agtttagctc tcttgactag tttcaaaaat ctgttaaaaa atgagaaaac gagtctcatc
      721 gagtgagaaa aaacccgttt aaaaaatata caaattaaac taaaagagag gtgtttatga
      781 aaaagaaaaa taatatagag tatttacagt taaacaatgc tgaaaatagt gaggattttg
      841 atattgaaca caagaaaaat cctttcgtgt tcaatatgga aattgatact aaaaagaaaa
      901 agctaactgt tgatcgaggt tctgaattag aaaccgaaga aggagttttt actacaacaa
      961 tttcacaaat ccaagaagta gataaagagg aatttttaaa gctgtttact ggtcaaatta
     1021 aattctattt cgatttgact caaacagggt ttaaattatt tttcttgatt atgggtattt
     1081 accaaaagca aatcggcggt gacgaaattt acttaaattt cgaaacagca aaggaagaag
     1141 ctgaaaaata tgattatact atttcaaaag ctgtttatta tcgtggatta aaagaactat
     1201 tagaaaagaa aatgatcgca aagtctgtta agaaatacat attttatata aatcctgcaa
     1261 ttgtatttaa tggcgatcgt gcaaggtttg ttaaagagat ccgaattaag gaagaagaaa
     1321 aacaaagaga agcagattta aaaagcatta atgaaagatt ggatcgatat gcttaaaaaa
     1381 ccaaaaccca acgggaaatt tttcactgtt tcccgttccg ggctttataa ttttaaagcc
     1441 tttggcttat tctggggtgt gtagttatta ttttgctgtt tctgtgaata ttcggcatct
     1501 gctgctgcaa tagcagcatt gaagagttgt ttaaattctg ccggtttatg ctcttgtatt
     1561 agatctaaaa catcactgtt aattttatat ttttcatatc tctgagaaat tgaagcattt
     1621 tcctttaact gtcttttgta ttctattatt tccaactcca tatcttttag ttttctgttt
     1681 ttattttgaa tgatttgttc ttgctcagcc gtggctctgg ccactgctaa cttttgctcc
     1741 tgttgtaccc gttcctggac tcttctgttg aagttctgac ggagatctga caactcagcc
     1801 tctgaatgct ctaaacggct cttaaacccg tttagacgag ctataagggg ctttcgttcg
     1861 ttcttgtaag gttttagagc ctttcgtatg taacgattaa cctcagctcc tgtgtagtgc
     1921 ttaggcacta cctccggatc tctaaataat tttttctttt cttctgtttc aacaactcta
     1981 attttttcaa ttttattatt ttctatttct aattcggttt cattcttaat gtttttaata
     2041 tcgctgtaaa attctttaac ttccttatat tctgcattcg aaacctcttt tttaatccca
     2101 cggattaaac ctaatggctt attgtattta aaatatatat cctgcattgt ttcatatttt
     2161 ttcatatacg atttattgtt tagtttatat attccatttt tattttcgat tggagttata
     2221 aaagcgtgaa tatgtggtgt ttgctcgtct aaatgcaaaa ctgcatttat tgcattttct
     2281 ccatattcat tatgcaaata ttccatttga acttttatcc aatcctctaa atttttgcta
     2341 tttgcaaaaa attccggaga agctgttaaa actaattcat tacaaataac agaagtagaa
     2401 tttcgagctt taacattggt tgcttgaaac cttgcattaa tatcagtcct taaatcacca
     2461 gaaccgatta aaattcggtt ttgggattta aggttaggat ctgcattatg tgttttcctc
     2521 aatcgcatat tgtgagaatt ttttcc
//
