LOCUS       NZ_CP129502             2388 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Enterobacter asburiae strain MO13m20 plasmid p2MO13m20, complete
            sequence.
ACCESSION   NZ_CP129502
VERSION     NZ_CP129502.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35153056
            Assembly: GCF_030444535.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     VIM-1-producing Enterobacter asburiae with colistin resistance genes
            from wastewaters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2023) Center for Structural Biology, Vrije
            Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 13:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,355
            CDSs (total)                      :: 5,242
            Genes (coding)                    :: 5,146
            CDSs (with protein)               :: 5,146
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 20 of 96
            Pseudo Genes (multiple problems)  :: 20 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129502.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="MO13m20"
                     /isolation_source="water"
                     /db_xref="taxon:61645"
                     /plasmid="p2MO13m20"
                     /geo_loc_name="Czech Republic: Brno"
                     /lat_lon="49.130318 N 16.633070 E"
                     /collection_date="2016"
     gene            complement(45..362)
                     /locus_tag="QOM23_RS22925"
                     /old_locus_tag="QOM23_22925"
     CDS             complement(45..362)
                     /locus_tag="QOM23_RS22925"
                     /old_locus_tag="QOM23_22925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023224510.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEATGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            1718..1945
                     /locus_tag="QOM23_RS22930"
                     /old_locus_tag="QOM23_22930"
     CDS             1718..1945
                     /locus_tag="QOM23_RS22930"
                     /old_locus_tag="QOM23_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206659768.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSCMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            complement(1993..2181)
                     /locus_tag="QOM23_RS22935"
                     /old_locus_tag="QOM23_22935"
     CDS             complement(1993..2181)
                     /locus_tag="QOM23_RS22935"
                     /old_locus_tag="QOM23_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(537..1057)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.868"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgattggcag gtacaaaaaa agcccggcag ggcttttttg taccttattg atctgcacac
       61 tttgcagcct tcagggcttc cagctgattt agccggttag ctgctttttt gcgggcttcg
      121 ccttttgcca tgccatttcc tatcccaaaa tcacccagga aacctaatac ggtgcgcccg
      181 tcaaattcgc cggttgcctc aatttcttgt tgtacactgc gcgttttggc gatttccaga
      241 gctaccgctt tgcaatccat tgcggaagat tctggaccgg tgacgttcgc ggcctgtgga
      301 tattgctttg ttgcgcatcc tgccatgatc agcgctgccg ataggatgaa tatttttttc
      361 aaaattatcc ttatttattt aggcatagtt aagagatacc cattttatcg gcaggaaatg
      421 agggtttttt agttgaattt tgggaggggc aaggttccat attggtcgag gattctggag
      481 ctgaccggca acggccagcg cggctatggg ccatgcggtg gttgagcata gaaaggatct
      541 tcttgagttc ctttttttct acgcgtaatc tcttgctctg aaaacgaaaa aaccacctgg
      601 ggaggtggtt tttcgaaggt taagtcagtt ggggaactgc ttaacctggt aactggcttc
      661 agcagagcgc agataccaaa tactgtcctt ccagtgtagc cgtagttagg ccaccacttc
      721 aagaactcta tagcaccacc taaattaact cgctctgcta atcctgttac cagtggctgc
      781 tgccagtggc gctttgtcgt gtctttccgg gttggactca agacgatagt taccggataa
      841 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
      901 cttcccggaa ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga
      961 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg
     1021 cctggtatct ttaagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     1081 atgcttgtca ggggggcgga gcctatggaa aaccggcaac gccgcggcct tttcttttgc
     1141 ctctcgatcc tctctcattg cctgtgcctt aatgtctccg ttccctccgc tcgccgcagc
     1201 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatccccg gacttatctt
     1261 cggcaccgta cgcgccagtg ctgcattttt taatacgatg caggataggg caaacgccgc
     1321 aaaatgacgt catccgacat cattccgccg cgttcgtcca gtgacctaca gtcggctctg
     1381 gggtgtcggg gctgtgccct gaccaggcgt tttggagtat cgccaccgtg gtggcggtgc
     1441 tacaaaacat atcctgtccc ttccttggca tctctgaagt ttagccagta tacactccgc
     1501 tagcgctacg tgactggttc agggctgcgc cccgacaccc gctaaaagca ctgacgcgcc
     1561 tgcggcttgc ccaaccccgc gccgtccggg aaagaatttc ccgatcgacc cgacgtttct
     1621 gtttacctgt gatgttaacg tttggatctt tttcccgggt ttacctattt tcttctaatg
     1681 aagcgttcac agctgacgtt aatagatggg tgctttcatg tttggttttt caccaggaga
     1741 acttatgaac ctttgccccg atgaacgtct gctctttgtg cggatgattt ccgctatgct
     1801 tcgccgttcg ggcggtgatg cgggtgctgt tatgtttgag gcgtatcgcc atattgtgtc
     1861 cgataccaat caagctcgtc gttcctgcat gctcgacctg ctcgaaagcg tcaggcatga
     1921 ctatgtacac ggtggatata catagttgcc ctggatacgc ttttcgtcta agtgagcgtt
     1981 cttttcgtct ctttattcgc cgtcctgtat accgaagcgt gcgctcagct tctgataaag
     2041 cgcttctgcc tgttcgtgca ttcgctcaca gtcggctgcc tcgtcatcaa gatcgagcac
     2101 gtcgagtttt tcgagcagca gcagcgactg cgcccggata aattttgcca tgttcagtgc
     2161 ggctgattcc tgttttgtca tacggctttt tcccggtttt atggtgatcg cagactgaaa
     2221 tcttacttca ttcacttgag tgcatagcca tgcaggtacg cagaaatcat cagcgcgttt
     2281 ctgagcggtt ttcagggggt aagatgtgtt cttttgcagg tgaggtcgta gaaacgccct
     2341 gagagcctct caggcggtgc acgcaacaga tgttatggta aatccagc
//
