LOCUS       NZ_CP129501             1184 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Enterobacter asburiae strain MO13m20 plasmid p1MO13m20, complete
            sequence.
ACCESSION   NZ_CP129501
VERSION     NZ_CP129501.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35153056
            Assembly: GCF_030444535.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     VIM-1-producing Enterobacter asburiae with colistin resistance genes
            from wastewaters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Sukkar,I., Valcek,A. and Dolejska,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2023) Center for Structural Biology, Vrije
            Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/17/2023 13:17:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,355
            CDSs (total)                      :: 5,242
            Genes (coding)                    :: 5,146
            CDSs (with protein)               :: 5,146
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 32 of 96
            Pseudo Genes (incomplete)         :: 68 of 96
            Pseudo Genes (internal stop)      :: 20 of 96
            Pseudo Genes (multiple problems)  :: 20 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129501.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="MO13m20"
                     /isolation_source="water"
                     /db_xref="taxon:61645"
                     /plasmid="p1MO13m20"
                     /geo_loc_name="Czech Republic: Brno"
                     /lat_lon="49.130318 N 16.633070 E"
                     /collection_date="2016"
     gene            complement(519..1043)
                     /locus_tag="QOM23_RS22920"
                     /old_locus_tag="QOM23_22920"
     CDS             complement(519..1043)
                     /locus_tag="QOM23_RS22920"
                     /old_locus_tag="QOM23_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240432.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_160384966.1"
                     /translation="MFVNPELHGKKRQEQLDESAKKATREHEEAKKNSRFTQVSPKGWE
                     RVRELLTDKQGVAALRLYSFLAEHIDPACGAVVADQQFLADKMGVNRSTIIRWLNYLES
                     KNALVRIPVAGKVCAYALDPHEVWKGYNSSKDYAAFVTKTLVNNDGEIQRKIMAMFSGA
                     ESGQEQKHSDS"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ataaaaacaa ttacttagta tttttactat atctaatctt taaagactaa cagacaacac
       61 caaaagatct ggtagcaccc tcccttcggt cgggcatttt tggggtcaag gttcgccttt
      121 aggcgaccgc gaagcggctt gaccttgacg ccaaaaaaga ccgatcacac ttgcaggagg
      181 tggttgtgat cacgcagtga gcgcaaccac ctgtcagacg tttttggttt tgacctttgc
      241 gggtttcggg gcacagccct gaaccagtca cggtgaaccc tcgttagagg ggactacgtg
      301 tgtactggct tatcggtatt tggcatcgca gatcgcgcag tttcgaagaa actactaagt
      361 gcgccttctc aattttcaga cataaggatc gtgtgttgcc tacctagtct ttcgggtttg
      421 ttttttgatg ttcgagaagg tcaaaagcgg cttaacaggt ggatgggtca ctacgttctc
      481 catccacctt tgccaagtgt gggggtcact acaagccgtc aagagtcgct atgcttctgc
      541 tcttgaccgc tttccgctcc tgaaaacata gccatgatct tccgttgaat ctcgccatcg
      601 ttgtttacaa gcgtcttggt cacgaatgca gcgtaatctt tcgatgagtt ataacccttc
      661 caaacttcat gggggtcaag tgcatatgcg cagacctttc ctgcaactgg tattctaact
      721 aatgcgtttt ttgattctaa gtagttgagc catctaatta ttgttgatct attcacaccc
      781 attttatcgg cgagaaattg ttgatcagca acaacggcac cacacgcagg atcgatatgt
      841 tcagccaaaa atgagtaaag ccgtaacgcc gctaccccct gtttgtccgt gagaagctct
      901 ctaacgcgtt cccagccttt tggtgatacc tgggtgaacc tagagttctt ttttgcttcc
      961 tcgtgctctc tggtggcttt tttggcactc tcatcaagct gttcttgtcg ttttttccca
     1021 tgcaactctg gattcacaaa catggcattt tagtccccaa tttggatggt acattcctgt
     1081 ctcaaaaatg ctacatcttg ttctatttat gcgtcaaggt gttctgtatt gttgcacgca
     1141 gggtgcaaca acgttgttgc acgcagggtg caacaggtca aaca
//
