LOCUS       NZ_CP129377            10430 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Staphylococcus epidermidis strain CSMH-411EB plasmid pCSME411-3,
            complete sequence.
ACCESSION   NZ_CP129377
VERSION     NZ_CP129377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36269258
            Assembly: GCF_030435955.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Lee,G.Y. and Yang,S.-J.
  TITLE     Genome sequence of a S. epidermidis isolate recovered from a chicken
            meat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Lee,G.Y. and Yang,S.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Department of veterinary microbiology, Seoul
            National University, 1, Gwanak-ro, Gwanak-gu, Seoul 08826, South
            Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Prokka v. v1.14.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 552.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 15:52:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,412
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,267
            CDSs (with protein)               :: 2,267
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 38 of 61
            Pseudo Genes (incomplete)         :: 32 of 61
            Pseudo Genes (internal stop)      :: 25 of 61
            Pseudo Genes (multiple problems)  :: 24 of 61
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129377.1.
            Bacteria and source DNA available from Seoul National University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="CSMH-411EB"
                     /isolation_source="Chicken meats"
                     /db_xref="taxon:1282"
                     /plasmid="pCSME411-3"
                     /geo_loc_name="South Korea: Gyeonggi"
                     /lat_lon="37.2 N 126.9 E"
                     /collection_date="2019-06-01"
                     /collected_by="Chung-Ang University"
     gene            complement(join(9618..10430,1..48))
                     /locus_tag="QYE82_RS11985"
     CDS             complement(join(9618..10430,1..48))
                     /locus_tag="QYE82_RS11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_046026634.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYSPETRNINRFVDDLQRVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     ERYLEIATSPDLKYILNSITSNFTKFELKEMTHLKSTYAKNMFRILKQYKHTGYMKIKI
                     EDFRERLDIPNSYRMSNINQFVLNPIIKELSTIFKNLHINKIKAKKGRKIEWLEFTFDA
                     EKRIHSKQKPQMANIGKSRQYISREKTPKWLEERTYGQQLQSDFDPQLEKDKEEFLKHL
                     KLDWE"
                     /name="RepB family plasmid replication initiator protein"
     gene            659..1162
                     /locus_tag="QYE82_RS11990"
     CDS             659..1162
                     /locus_tag="QYE82_RS11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020367619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301560589.1"
                     /translation="MNKPVNLKKLIALTLSVLLLCSVISIFFNENQSQAKGNGGYVIYK
                     VTNNSPRGSVSYKQADVFMTSSQAKKFASENSLSIKQALVNAGLGLIPGNYATYFGITK
                     SVGDVVQSKMVYDQAKKGPVRITMEKGGTIKKVTKWNGKTKKPSVSSFNKGQVSQTVKV
                     LSKK"
                     /name="hypothetical protein"
     gene            complement(1899..2618)
                     /locus_tag="QYE82_RS11995"
     CDS             complement(1899..2618)
                     /locus_tag="QYE82_RS11995"
                     /inference="COORDINATES: protein motif:HMM:NF015839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="WP_301560591.1"
                     /translation="MKQLNEIQASLNQIGTNSPKTQMSLSVADKLQNQQDLLMKRLEEI
                     EKREHERNKRHDELLTTIETTASNFNQATEITQKRFISVAKHYIERINNDNLKQDFQTA
                     IQEELKDVKTDTHKAIEQLQTNQAELRQANNDYKKMIDERIKHNDTAMKQYDQAFHRLT
                     QGITAMFFIIALVMVTFLVLSPLGDWLGLQHFYEWLNHVLKTSHSIWRYLMIVFYLVPY
                     ALFGLLIYAILSAYKRI"
                     /name="DUF334 domain-containing protein"
     gene            complement(<2752..3417)
                     /locus_tag="QYE82_RS12000"
                     /pseudo=""
     CDS             complement(<2752..3417)
                     /locus_tag="QYE82_RS12000"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3600..3800)
                     /locus_tag="QYE82_RS12005"
     CDS             complement(3600..3800)
                     /locus_tag="QYE82_RS12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064593626.1"
                     /translation="MAKPKLRLTFNIIIGILALTYIGLAIFFDFTNNIWFMAFLFIVMI
                     LHILKAIFAPKKNRSDEYRNY"
                     /name="hypothetical protein"
     gene            complement(3782..4165)
                     /gene="mobC"
                     /locus_tag="QYE82_RS12010"
     CDS             complement(3782..4165)
                     /gene="mobC"
                     /locus_tag="QYE82_RS12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_064593623.1"
                     /translation="MSEQNTFVASDETVGRNRKPNRKAPKQISFRVSESEYLKLQQSAE
                     TLNMSVPAFVKKKAQGARLVAPKLDQATRQSVAKDLSMLGANANQIAKYCNQHQHEAPN
                     YEALERNISELRERLNDIWQSLN"
                     /name="mobC"
     gene            4562..4765
                     /locus_tag="QYE82_RS12015"
     CDS             4562..4765
                     /locus_tag="QYE82_RS12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008797753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002458745.1"
                     /translation="MKRHLLNALMFAIVIFLADAFKHLLIYFSALRIDFTTNSALDILI
                     SVILGFIFGLFVSWGLDLKKNK"
                     /name="hypothetical protein"
     gene            complement(4826..5440)
                     /locus_tag="QYE82_RS12020"
     CDS             complement(4826..5440)
                     /locus_tag="QYE82_RS12020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_301560590.1"
                     /translation="MSFKKRIIASISLVLLLGFSTETFVDAQEIHNRENNQTQVQQNNQ
                     TNQEDIDRLASVLKVLNEHKDAIVFNEDNKSVKFKASVLQQEMSVSEYNKVITDLDKEG
                     LVANETSYRRGVGQGMPDTRTKKQRKRQEYVDNCIGNELSKTYGIKASKSLIQVIKSKD
                     YKKAAKEILKKETKATNVVFTLSKMLIKCQNKGVKKYGSGS"
                     /name="hypothetical protein"
     gene            5606..6181
                     /locus_tag="QYE82_RS12025"
     CDS             5606..6181
                     /locus_tag="QYE82_RS12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_046026646.1"
                     /translation="MKKILIIIVCLLVVLTACSNQESKNNKDKIIIYGDFKCPYCKQLE
                     EEIVPKLKKEYIDKDKVKYKFVNMAFLGEDSIKGSRAGHAVENIAPKQYFKFQKLMFSK
                     QPNNEKEWITEKLIDQQIDKLNISHRKAEKIKTDYKKKNSQSWKDAKKDQKTCKKKNIQ
                     EAPTVFINGKEVQNVYDYQEYKKYLKNE"
                     /name="thioredoxin domain-containing protein"
     gene            complement(6364..6819)
                     /locus_tag="QYE82_RS12030"
     CDS             complement(6364..6819)
                     /locus_tag="QYE82_RS12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020362985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020362985.1"
                     /translation="MKVKRHLFDLDESGSREFDFSILIIMLCIAFRNYFKGYGFAIHLN
                     SDLLFEMTLLLLPISLLFFNKMTDKDVANIFKSYTSLLFPFIGGIIYMIIASFLIYIAR
                     FIPFIDFLFSLIGLAILIAGIINILIAILIFLTKKRVKQKESKKVNN"
                     /name="hypothetical protein"
     gene            complement(6842..7030)
                     /locus_tag="QYE82_RS12035"
     CDS             complement(6842..7030)
                     /locus_tag="QYE82_RS12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002457894.1"
                     /translation="MDLKTVNQRIAEKEQQIKELSRQMSNKRNSMKTTQSYTHFYKQLH
                     ILENQLKGLEKLKKKLK"
                     /name="hypothetical protein"
     gene            complement(7603..7692)
                     /locus_tag="QYE82_RS12040"
     tRNA            complement(7603..7692)
                     /locus_tag="QYE82_RS12040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7654..7656),aa:Lys,seq:ttt)
                     /name="tRNA-Lys"
     gene            7775..8254
                     /locus_tag="QYE82_RS12045"
     CDS             7775..8254
                     /locus_tag="QYE82_RS12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020367477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="WP_301560588.1"
                     /translation="MLKKLITCTLLSSIALTGVNMLEYSPKIDQNHIAQAAEIDKYPRL
                     IKKQQPNYHTKNNCTWYVHNKRNQTKRYLPSSFTDAKNWLNEAKRAGFATGKKPIAGSI
                     LQTSKGGGGYGHVAFVEKVNKNGSIKISEYNYNVRLGYGTRTLTKKQAAQYNYIY"
                     /name="CHAP domain-containing protein"
     gene            8325..9560
                     /locus_tag="QYE82_RS12050"
     CDS             8325..9560
                     /locus_tag="QYE82_RS12050"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020364758.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071555 - cell wall organization [Evidence
                     IEA]; GO:0009252 - peptidoglycan biosynthetic process
                     [Evidence IEA]; GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="WP_020364758.1"
                     /translation="MKFVTLTEQEYQLFIEKHVVHYTQSIEHYNYRQKHKKDVHLVGVK
                     DNEEVIAACLLSEAQVLKIFKYFYSHRGPVLDFNNEKLVECFFTGLTRYLKRHKGLFVL
                     VDPYILENNRDADGRILKSYDNSNLINQLNHLGYQYQGHSIGYSDTSQIRWLSVLDLEG
                     KNEKDILNEMDYQTRRNIKKTYEMGVEVKTLDISQTDQFFELFRMAEEKHGFKFREKDY
                     FEEMQNIYNNNSALKLAYIDLKKYLNQLNNKELVLNEQIEEIKAKLKDNPNSKKNKNKY
                     QELNKQLTSNRKHITETENLIKIDGDILHLASAYYIWNKDEVYYLSSGSNPKYNQFMGA
                     YRLQWDMIKFALNNNIPRYNFYGITGDFSENAEDYGVQQFKKGFNAHVEEYVGDFIKPI
                     RPIFHKVYKLLK"
                     /name="aminoacyltransferase"
     Typing          4137..4375
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.021"
                     /coverage="100.0"
                     /evalue="1e-101"
     Typing          complement(9617..10430)
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.7"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          complement(2411..3378)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.046"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 aggcactaag ttcatttcgt ttttatatat aacggtttct ccactcatat aatactcctt
       61 ttatacaaaa aggcatagtt tttttgttgc tatgcctttt taattttaaa aatatattta
      121 taaaccttac caataaagta acaaaacata aaaggcatag caacaaaaaa actatgcctt
      181 ttatgtggca aaactatgcc ttttatgtgg caaaactatg ccttttatgt ggcaaaacta
      241 tgccttttat gtggcaaaac tatgccttta acatctttga ttcattgtcg ctctaggggt
      301 tacatagtgc ctaaaaaggt tttaaaaaga tttataaaaa gatattaaaa agttcggggc
      361 atttgaccct tacaaattta aaataaaaaa agatacagaa aagtatacga aatataaaat
      421 aaacttcaaa aataaaactc ctttctttca aaaaaattta tcacaaatag tatttaaatc
      481 ttaaatgccc ttatttttaa ttttaaagga gtattttgaa gtattagggg ttatttatat
      541 agttttaaaa attaatacga agtcattaaa tatattaata aatatatcat ttatattcct
      601 tgtgtattat tttacacttt ccttgtacga aaaaaacaag ttaaaaggtg gtaaaactat
      661 gaataaacct gtaaatttaa aaaaattaat tgcactaact ttatcagtat tattactttg
      721 cagtgttata agtatttttt ttaacgaaaa ccaatctcaa gcaaaaggaa atggtggata
      781 tgttatatac aaagtaacta ataacagtcc aagaggttca gtatcatata aacaagctga
      841 tgtctttatg acaagtagtc aagctaaaaa gttcgcatca gaaaactcac tttctataaa
      901 acaagcttta gttaatgcag gtctaggact tatacctgga aattacgcaa catattttgg
      961 aattactaaa agtgttggcg acgttgtcca atcaaaaatg gtctatgatc aagctaaaaa
     1021 aggacctgtt cgtattacta tggaaaaagg tggtacaata aaaaaagtaa ctaagtggaa
     1081 cggaaaaact aaaaaaccat ctgtatcatc attcaataaa ggtcaagttt cacaaacagt
     1141 aaaagtactt tcaaaaaaat aagttataag aaagcgtatt tcatttagaa atacgctttc
     1201 ttataacata atgtaaaaac caaaaaacta atatccatac catagtaaaa accttataat
     1261 tacctggcaa aatataagca gtaataacta taatcggaac gaaaattatt aatagacaaa
     1321 gatagacttt taaattcaat ttactcatat taaaatctcc ttctttattc atagataaac
     1381 ttcaaaattc tattcactac ttataaataa ctctaatatc taaacgctaa taaaaccatc
     1441 ttttttaata taaacttatt ttacattatg acaattgctt tattgacgtt gagcctcgga
     1501 acccttaaca aaccccaaaa ctgtcgactc ttcggtttca tagctcacgc tacaccgaca
     1561 ttcgtcgaaa cagtttaatt aagggttctt ctcaatgctc aataaatttt ctcggcataa
     1621 tagcgtgttc tattcttcat ttttcatctc ttttacaacc tttaatattg gcatgattag
     1681 taatgataaa ataaaggctg attctaaaat cagaaaaata tttatttctt taaaaaatat
     1741 taaatctatc acatatccaa aaagacaaaa tatgataaag agacaaataa aatgaacaat
     1801 tgatttcaag gctaatactc ctttttgctt tctaagttat tttaagcttg atatagcttt
     1861 tccattatca aaaacatggt tttggtccaa tattcatatt aaatacgctt atacgcgctt
     1921 aaaatggcat atataagcaa tccaaataaa gcataaggaa caagatagaa tacaatcatc
     1981 aagtaacgcc atattgaatg acttgttttt aatacatgat tcaaccattc ataaaaatgt
     2041 tgcaaaccta accagtcgcc taagggactt aagactaaaa atgtgaccat gactaaagca
     2101 atgataaaaa acattgctgt aatgccttgg gttaaacgat gaaacgcttg atcatattgt
     2161 ttcatggctg tgtcgttgtg cttgatacgt tcatctatca tttttttata atcattattg
     2221 gcttgtcgca gttcggcttg gttagtttgt aattgctcaa ttgccttatg cgtgtctgtt
     2281 tttacatctt tcaattcttc ttggatggct gtttggaaat cttgttttaa attatcgtta
     2341 ttaatgcgtt caatatagtg ttttgctaca ctgatgaaac gcttttgtgt gatttcagtc
     2401 gcttgattaa agttcgaggc cgtggtctcg atcgttgtta ataattcgtc gtgacgcttg
     2461 tttcgctcgt gctctctctt ctcgatctct tcgagccttt tcatcagcaa gtcttgctga
     2521 ttctgcagct tgtcggctac gctcaagctc atttgtgtct tgggcgaatt ggtcccaatc
     2581 tgattgagtg acgcctgtat ctcgttcagt tgcttcatca cgacttgaag ttcgtcgttt
     2641 gggttggacg tcagtgtctg atgttcgttc tgtttgttgt tgtcgttgta tttgtctttc
     2701 aaagccatct tcgatacctc ctttattgta atcttcaccg agtttacgcc cacgcacctt
     2761 tttatcttcg gctaaatgtc gataggtaat gcttttgggt gtgacacgtt caatttcaat
     2821 accatgttgg tttaaatggt ctttgaattc atccatattc gttgcttgtg attggtctat
     2881 cgcctctctg attttatctt tccatgaata ttgggcagtt gatttcgtat tagggtcagc
     2941 aatcgctttt tctgtctgcg tataacgtaa acgtgctgtg tctttgtctg gcacgcttaa
     3001 accgtgttcc atgcaaacct catcattgaa atctctgaca ttacgtaacg cttgtttatt
     3061 attgttaaat ttccgacctg tctcgaggtc aattgaatta atcacaatat gattgtgtac
     3121 atggtctgta tcggcatgcg tatagaccgc cacttgatga ttgggtgcca gtttttcagc
     3181 caattctaaa cctaactgat tacattgttc gggcgtgact tcgccagctt taaacgattg
     3241 gataatcaca tgcccttgat tcccatcggt tttgccatat aactcacgag aagctttaaa
     3301 actcgatttg gcatagtcga catcacaatt taagccactt ttttcaacgg ctcgtttctc
     3361 agcataatta atggcccgtg atgttgattt ggtcgcactt aatttagttg ttgccattgt
     3421 tatcacgctt tttctgtgta gggataaagt aatcaatgaa aaggccaccg aaagcacaaa
     3481 ataagaaaca gatagttata aaccaccatt gttttaggaa tgaaaaatta aaaaataaag
     3541 tcaaaatcat taaagatata acagttaaaa tccaaaggct aaatagcaat attcttagtt
     3601 tagtagttac gatattcatc actccgattc tttttaggtg caaaaatcgc ttttaaaata
     3661 tgcaaaatca taacgatgaa taaaaaagcc ataaaccaaa tattattagt aaaatcgaaa
     3721 aatatagcta aaccaatata agtaagtgcc aatataccga taataatatt aaaagttagt
     3781 ctcaatttag gctttgccat atatcgttca acctttcacg tagttcacta atgttgcgtt
     3841 ctaatgcttc gtagtttggt gcttcatgtt gatgttggtt gcaatatttc gcaatctgat
     3901 tggcatttgc gcccaacata ctcaaatctt tcgctaccga ttgtcgcgtt gcttgatcca
     3961 atttgggtgc gactaatcgg gcgccttgtg cctttttctt aacgaaagca ggcacactca
     4021 tatttaaagt ttcagctgat tgttgtaact ttaaatattc ggattcgctc acacgaaaac
     4081 tgatttgctt cggcgcctta cggttgggtt tacggtttcg cccaacagtt tcgtcgctag
     4141 ccacaaatgt gttttgttcg ctcattcaat cccaccacgc tttcttcgaa agataacgcc
     4201 tttcttacat tttactttat aggataaagt aaaattaatc aagtctttaa gtggtttggc
     4261 aagcactggc tttgctagcc actaacgtgg tagacgcgcc agcttgttgg ggataatccc
     4321 caagcccctt atgatttttt ctagcgaaaa aatagtggct aacatgttag ccacttaatg
     4381 ttgaaaatat aaaaattaat ttatgaaaga gtttactata ctcaatatta tattacttca
     4441 acttttcttg tgatgagtta tcttataatc ttactgttta tatatttgat attgtattta
     4501 gtgttataca tcttgtatta tcgtaattgt taagaaaatt ttaaaaagaa aagaggatat
     4561 tatgaaaaga catttattaa atgcacttat gtttgctatt gttatttttt tagcagatgc
     4621 atttaaacat ttacttattt attttagtgc attgagaata gattttacta ccaatagtgc
     4681 cttggatatt ctgataagtg ttatattagg atttatattt ggtttgtttg ttagctgggg
     4741 attagatttg aagaagaaca aataaaatta ggcccgtcat ctaaatgagg ggcctaattt
     4801 tatttgttag ataaatataa tgttattagc ttccagatcc atatttttta acgcctttat
     4861 tttggcattt tataagcatt ttacttagtg tgaaaaccac gttagttgct ttagtctctt
     4921 tttttaatat ttctttagca gcttttttat aatccttaga ttttataact tggataagtg
     4981 atttagaggc tttaattccg tatgtcttac taagttcatt acctatacaa ttatctacat
     5041 attcttgtct tttacgttgt ttcttagttc tcgtgtcagg cataccttgt ccaacacctc
     5101 gacgatagct agtttcatta gcaactaatc cttctttatc taaatcagtt attactttgt
     5161 tatactctga aactgacatt tcctgttgta aaacagaagc tttaaattta acagatttat
     5221 tatcttcatt aaatactata gcatctttgt gttcatttaa tactttaaga acactagcta
     5281 atctgtcaat atcctcttga ttggtttgat tattttgttg aacttgtgtt tgattgtttt
     5341 cacggttatg tatttcttgt gcatctacga atgtttctgt agaaaagcct aataaaagaa
     5401 caagagatat tgaagcaata attctttttt taaatgacat ttttagtttc tctccttttt
     5461 tttaaatact agtaaatttt atcatttaat aaaaaaattt ataagcactt tttgtatatt
     5521 ttacataaaa taatttaaag gtgtaaaaaa gctgttatta agataatatt atgatgttca
     5581 tcatatatag agaggaaata taaaaatgaa gaaaatttta ataataattg tttgcttatt
     5641 agttgtttta actgcatgta gtaatcaaga aagtaaaaat aataaagata aaattattat
     5701 atatggtgat tttaaatgtc catattgtaa gcaattagag gaagaaatag tacctaaatt
     5761 aaaaaaagaa tatattgaca aagataaagt gaagtataaa tttgtaaata tggcattttt
     5821 aggcgaagat tcaattaaag gctcacgggc tggtcatgct gttgaaaata tagcacccaa
     5881 acaatatttt aaatttcaaa agttaatgtt ttctaagcaa cctaataatg aaaaagaatg
     5941 gattacggaa aaattaattg atcaacaaat agataaactt aatataagtc ataggaaagc
     6001 tgaaaaaata aaaactgatt ataaaaagaa aaatagtcaa tcatggaaag atgcaaaaaa
     6061 agatcaaaag acttgtaaaa aaaagaatat tcaagaagca ccgactgtat tcattaatgg
     6121 aaaagaagtt caaaatgttt atgactatca agaatataaa aagtatttaa aaaatgaata
     6181 acctatacaa agtaactacc cataatcgtt aattatgtca acttaacaca aagggacaaa
     6241 cgctgaaatg acggcgtttg tccctttgtg tttggattat cgattattca tattttatac
     6301 atacccttaa ccacacgttt tcatgcattt tcattaagag aacttttgta tctttattca
     6361 gtctcaattg tttacttttt tagactcttt ttgttttact cgtttttttg taaggaatat
     6421 taatattgca attaaaatgt tgataatacc agcaattaaa atagctaaac ctatgagact
     6481 gaaaagaaaa tcaatgaacg gtataaaacg agcaatgtat atcaagaatg aagctataat
     6541 catatagata atgccaccta taaatgggaa taaaagagaa gtatagcttt tgaatatatt
     6601 tgcgacgtct ttatctgtca ttttattaaa aaataagagc gaaataggaa gtaataataa
     6661 ggtcatttca aacaataagt ctgaatttaa atggattgcg aatccataac ctttgaagta
     6721 gtttctaaat gcgatacata gcatgatgat taatattgaa aaatcaaatt ctctactacc
     6781 actttcgtct aaatcgaata aatgtctttt aactttcata gtaatccctc gctttgaatg
     6841 attatttcaa tttctttttt aatttttcta agcctttaag ctggttttct aagatgtgaa
     6901 gttgtttata aaagtgagtg tatgattgtg ttgttttcat tgaattacgt ttattactca
     6961 tttggcgtga caattctttg atttgttgtt ctttttcagc aatacgttga ttcactgttt
     7021 taagatccat aataacctcc ttgacatttt tatataaaaa ataaatacaa aacaataatg
     7081 ataaatattt tattattata cattataaca ctaaaatgta taataatgtt atttgtatgt
     7141 aaattgtcat taaaaatacc caactaatca attttgatta gttgggtatt aacaataatt
     7201 gagcgcctaa aattcatcta ttttctagac gttggtgatg ttatatgttc aagtgttgca
     7261 tactgtatgc atgtatattg tatgcgggca tacatatcat gtgaactcgt acagtacaga
     7321 gagattttgt tataattggg aagtcgtcta tctctcagac gtcaatttga cgtagagagg
     7381 aggtgcgtgc tcatgactgc aatatttgtt tatatcatag tatctatttt tatagattgc
     7441 tgtggtgtgt tgtttcgctt ttggctagaa caacgcagta aaaaataggc gacatagcca
     7501 aacacctaaa aaccccaacc tatggcagta ggttggggtt tggtgcgttc atgacgcaat
     7561 atttgttaaa tctattatat tctttcttat actttttgta aacggaggga gaaggatttg
     7621 aaccaacgcg agcaaaaagc tcctaccaaa cacaaagtgt tggcctctta aaccagactt
     7681 gagtacccct ccataataat attaaatttg tatattattt acttttaata taatattata
     7741 aacggggtct gaagtcaagg aggaaaattc tttgatgtta aaaaaattaa ttacatgcac
     7801 tttattatct tcgatagcgc taacaggtgt taatatgttg gaatatagtc caaaaattga
     7861 tcaaaatcat atcgctcaag cagcagaaat cgataaatat ccacggctga taaaaaaaca
     7921 acagccaaac tatcatacga aaaataattg tacgtggtac gtacataata aaaggaatca
     7981 aacaaaaaga tatcttccat ctagttttac agatgcaaaa aattggctta atgaagccaa
     8041 aagagctgga tttgctacag gtaaaaaacc catagctggc tctattcttc aaacttctaa
     8101 aggcggtggt ggttatggcc atgtcgcttt tgttgaaaaa gtaaataaaa atggaagtat
     8161 aaagatttca gaatacaatt acaatgtacg tttaggttat ggcactcgta ctttaactaa
     8221 gaaacaagct gcgcaataca attatattta ttaattagaa cttcagaccc taaatcctgg
     8281 agttgggctc caggatttat attttaaatg aaaaaggtgg aaaaatgaaa tttgttacat
     8341 taacagaaca agaatatcaa ttatttatag aaaaacatgt tgtacattac acgcaatcta
     8401 ttgaacatta taactataga caaaaacata aaaaagacgt ccatttggta ggtgttaaag
     8461 acaatgagga agttatagct gcctgtttac tatctgaagc acaagtttta aaaatattta
     8521 aatattttta ttctcacaga ggtccagtat tagattttaa caatgaaaaa ttagttgaat
     8581 gtttttttac tgggttaact cgatatttaa aacgacataa gggattattt gttttagttg
     8641 atccttatat attagaaaat aatagagatg cagatggacg tatattgaag tcgtacgata
     8701 attccaattt aataaatcaa ctcaaccatt taggctatca atatcaaggt catagtatag
     8761 gctattctga tacaagccaa atacgctggt tatctgtttt agacttggaa ggcaaaaacg
     8821 aaaaagacat tttaaatgaa atggattacc aaactagaag aaatattaaa aagacatacg
     8881 agatgggtgt agaggttaaa acactagaca tttcacagac agaccaattc tttgaattgt
     8941 tccgaatggc agaagaaaag catggcttta aatttagaga gaaagattat tttgaagaaa
     9001 tgcaaaacat ctataataat aattctgctt tgaaattagc gtatatagat ttaaaaaaat
     9061 acttaaacca gttaaataac aaagagttag tgcttaatga acaaatagag gaaattaaag
     9121 caaagctaaa agataacccg aattcaaaaa agaataaaaa taagtaccaa gaattaaata
     9181 aacaattaac aagtaaccga aagcatatta cagaaactga aaatttaatc aaaattgacg
     9241 gggacatact tcatttagcg tcggcatatt atatctggaa taaagatgag gtttattatt
     9301 tatcaagtgg atctaaccct aaatataacc aatttatggg ggcatatcga ttacaatggg
     9361 atatgataaa gtttgcatta aataataata taccgagata taatttttat gggattactg
     9421 gagattttag tgaaaatgct gaagattatg gtgtgcagca atttaaaaaa ggttttaacg
     9481 cccatgtcga agaatacgtt ggagatttca tcaaacctat acgccctatt tttcacaagg
     9541 tttataagtt acttaaataa cacaataaaa ttaggatgtt cctatataga aacatcctaa
     9601 ttttattgtg ttatttatta ttcccaatct aatttaaggt gctttaaaaa ttcttcttta
     9661 tctttttcta gttggggatc aaaatcgctt tggagttgtt gtccatacgt tcgttcttct
     9721 aaccattttg gcgttttctc acgactaata tattgtctcg atttacctat attcgccatt
     9781 tgtggctttt gtttactgtg aatgcgtttc tcagcatcaa aggtaaactc taaccactca
     9841 attttacgac cttttttcgc tttaatttta ttaatatgaa gatttttaaa tatcgtagat
     9901 agttctttta tgattggatt tagaacaaat tgattaatat tagacattct ataagaattg
     9961 ggaatatcta gacgttctct aaaatcttca attttaattt tcatataacc agtgtgtttg
    10021 tattgtttaa gtattctaaa catatttttg gcataagtag atttgagatg tgtcatttct
    10081 tttaattcaa atttagtaaa attactagta atggagttta aaatatattt taaatctgga
    10141 gaagtagcaa tttctaaata tctttcagat atatcaattt catatttata aaacagaacg
    10201 aattttttta ttttcgtttc tgttctaata gtatatcgaa tatctaacat tttatcataa
    10261 actctttgta agtcatctac aaatctgttt atattacgtg tttctggact ataattactt
    10321 aattctttta gttcatcaaa agacaaatgt aatgtattag tgtcttgctc ttttaattta
    10381 ttacacatgg caaaaaataa gttaatttca gttgctgtaa atcgtcttaa
//
