LOCUS       NZ_CP129367            10030 bp    DNA     circular CON 19-JUL-2023
DEFINITION  Staphylococcus epidermidis strain CKFA-393 plasmid pCKFA393-2,
            complete sequence.
ACCESSION   NZ_CP129367
VERSION     NZ_CP129367.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36269254
            Assembly: GCF_030435915.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Lee,G.Y. and Yang,S.-J.
  TITLE     Genome sequence of a SCCmec V carrying S. epidermidis isolate
            recovered from a broiler
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Lee,G.Y. and Yang,S.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) Department of veterinary microbiology, Seoul
            National University, 1, Gwanak-ro, Gwanak-gu, Seoul 08826, South
            Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Prokka v. v1.14.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 484.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 15:50:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,329
            CDSs (total)                      :: 2,245
            Genes (coding)                    :: 2,191
            CDSs (with protein)               :: 2,191
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 30 of 54
            Pseudo Genes (incomplete)         :: 34 of 54
            Pseudo Genes (internal stop)      :: 16 of 54
            Pseudo Genes (multiple problems)  :: 20 of 54
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129367.1.
            Bacteria and source DNA available from Seoul National University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="CKFA-393"
                     /isolation_source="cloaca"
                     /host="Gallus gallus domesticus"
                     /db_xref="taxon:1282"
                     /plasmid="pCKFA393-2"
                     /geo_loc_name="South Korea: Gyeongsang"
                     /lat_lon="35.4 N 128.9 E"
                     /collection_date="2019-05-19"
                     /collected_by="Chung-Ang University"
     gene            231..1097
                     /locus_tag="QYE85_RS11580"
     CDS             231..1097
                     /locus_tag="QYE85_RS11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440631.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_237616286.1"
                     /translation="MSGETVIYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLHL
                     SFDELKELSNYNPETRNINRFVDDLQRVYDKMLDIRYTIRTETKIKKFVLFYKYEIDIS
                     ERYLEIATSPDLKYILNSITSNFTKFELKEMTHLKSTYAKNMFRILKQYKHTGYMKIKI
                     EDFRERLDIPNSYRMSNINQFVLNPIIKELSTIFKNLHINKIKAKKGRKIEWLEFTFDA
                     EKRIHSKRQPQMANIGKSRQMTSREKTPKWLEERDNPKYQNKYNVDENFEKDKEAFLKQ
                     LKVDWEE"
                     /name="RepB family plasmid replication initiator protein"
     gene            1191..1916
                     /locus_tag="QYE85_RS11585"
     CDS             1191..1916
                     /locus_tag="QYE85_RS11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_237616285.1"
                     /translation="MKITKFFTLFLLFLTITIVLSGCNKDVEHKKNISIKDIINDKKQH
                     MFFSMSTDEDNNNEIESIYLTKNGQMKEICLGSSEISPSFLLNLDPNEVKKVLDNKKID
                     YQETPYKDVKTYLEVTNEKGNALITFVTTKYISEKKAKKGLGDVLKSVYSNDKKTEGLL
                     LYTNVSTGSIQSAGDAGKYTGQLTNDNLPSLMNESNDESDRLDTIIKLNNKDQKMVNVS
                     SQDKDVIKIKLSDFDTKLD"
                     /name="hypothetical protein"
     gene            complement(2511..2876)
                     /locus_tag="QYE85_RS11590"
     CDS             complement(2511..2876)
                     /locus_tag="QYE85_RS11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494365.1"
                     /translation="MKWLKIIILILSLVPIEFIGLFTDYQTGLLIGYIPFIIVAILISR
                     SIFKFGLKNNISIIISRCIGTFLSWECVHWFMNIYDSSDYFKPLTTDIFALFLGAIHFI
                     VIMLIYLVIYGFSHRNN"
                     /name="hypothetical protein"
     gene            complement(3006..3374)
                     /locus_tag="QYE85_RS11595"
     CDS             complement(3006..3374)
                     /locus_tag="QYE85_RS11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002503834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_002494366.1"
                     /translation="MKKILKILITSIIVIIITLTVWTFSVITYQEHKSEKIIDHVIERK
                     GWDKKIKNEKMSFNIMMGYAEKDIAFKDQPYSEYEYNVTPAPAPWTDDKEYKVWGETDL
                     QKKDSYYKYLLESEPYRK"
                     /name="DUF3139 domain-containing protein"
     gene            3478..3873
                     /locus_tag="QYE85_RS11600"
     CDS             3478..3873
                     /locus_tag="QYE85_RS11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="WP_002494367.1"
                     /translation="MKRKYKITGIIFIVLLIVLTLIFSIVHHYANVQKHEEAKLKQKVH
                     HIFKQKGWEDKVKEEKNIFTFNTGDNDLQVTFKDEPYNTYTYSIDEDNKVYGNAVLKDE
                     YDKDFDSKKKYKEYLRKMHFEEKYDLK"
                     /name="DUF3139 domain-containing protein"
     gene            4485..4934
                     /locus_tag="QYE85_RS11605"
     CDS             4485..4934
                     /locus_tag="QYE85_RS11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494368.1"
                     /translation="MKKLLSVFIASLLVLTGLSFSSINQGNTASAKTKYKTTITYKGQK
                     YVYVGHYKHHFSKKEVKKVVKFSKGVHSGNKLVSAASKLSKNGYVKASSAIYKAFDFGL
                     KNELKGSYFYTAAKKGTGVTISYDMYTATTGPNHTIAKNFKHSYN"
                     /name="hypothetical protein"
     gene            complement(5094..5480)
                     /locus_tag="QYE85_RS11610"
     CDS             complement(5094..5480)
                     /locus_tag="QYE85_RS11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494369.1"
                     /translation="MKKAIFSIIISLILVLTVTGCSNSSKEKPIKKSALEINPTSKAVN
                     ITINKKENNKPEKIGKVYRYKNNNAKEITNDGIKKDTKDTLIWKGVANKYDNVKDLLGE
                     SILYEVKYKNGDIKKFERKIKYTE"
                     /name="hypothetical protein"
     gene            complement(5500..5661)
                     /locus_tag="QYE85_RS11615"
     CDS             complement(5500..5661)
                     /locus_tag="QYE85_RS11615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162153024.1"
                     /translation="MNEREQRQKLNITLPPKLITNLDKVKTKYGINKSKQIENLISKYL
                     NEEYGNLE"
                     /name="hypothetical protein"
     gene            6510..6884
                     /locus_tag="QYE85_RS11620"
     CDS             6510..6884
                     /locus_tag="QYE85_RS11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494371.1"
                     /translation="MKKIVYILGTIIVLGFCYFYVNLIFFDSWTRYSAEKEINNYISNH
                     DTKKLKEISANNKTYQLLKHAKHVTIKGDTDNQGGGHIGYFTSTINGKEGALFVHLNFG
                     LFPEIPKVTKLEVFDKNIYE"
                     /name="hypothetical protein"
     gene            complement(7475..7927)
                     /locus_tag="QYE85_RS11625"
     CDS             complement(7475..7927)
                     /locus_tag="QYE85_RS11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002494373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002494373.1"
                     /translation="MPKKQSSKEKILTVRVSQAQYKKIQDLAYIRSMNVTEYIRQTAVG
                     NRIKPTVIEYPKEQITMDDSNSKNNTNNLYKVIEHLEEDNEALKSDRDALRQRHDQLED
                     DIEQYKRENEIFHRLLEHFDSTAFMNFNTYRDDRPLKKEIKRLKEH"
                     /name="hypothetical protein"
     gene            8382..9590
                     /gene="mobV"
                     /locus_tag="QYE85_RS11630"
     CDS             8382..9590
                     /gene="mobV"
                     /locus_tag="QYE85_RS11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002958641.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_237616284.1"
                     /translation="MKLAWNTQKNKMSDVKGKEMEQERKGRIKNDEIDLNRTNLNFDLV
                     EDERHLYHRVKDRIEYYKKQGSRVQKNSVVMYSNIITLSKEEADRMGETRTKHYFKTCK
                     DYFSERFGEANVVSAKVHMDESAPHMHLHFIPVNHQGRLSARTAMNRQAINHIHDELTT
                     HLCQQGFDVERGSTDDNKTYIQDIHEYKKKAKFLSELDQQIETKKQLLRDINDLHDEKE
                     NLTRDIQNLKRKNHFLQEDSQQTQEDIDLYKQLRTISRDDFKKDEEKYRKEIEAYEASL
                     SGLRMNYRDLDKRNQHLTTQNNQLEHHLDMLNARHQAMNDMVRNDTRILFAGLRCALKD
                     MNIELSAEIKYQRMPEVGIDPDTVDQGALTHAYQGDVSGIDHHIRKASAPIKKETENDL
                     ELE"
                     /name="mobV"
     Typing          232..1096
                     /name="rep_cluster_1448"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.218"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8311..9587
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.479"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgaaatgtt aaaggcatag ttttgccaca ttaaaggcat agttttgcca cattaaaggc
       61 atagttttgc cacattaaag gcatagtttt gccacattaa aggcatattt tttttattgt
      121 tatgccttta actatatgtt accttaatta taagggttat gaggtgtttt aaaaatttac
      181 aaaggcatat tttttttatt gttatgcctt tgtaaagaga ggagtatcat atgagtggag
      241 aaactgttat atataaaaat gaaatgaact tagtgccttt aagacgattt acagctactg
      301 aaatcaactt attttttgca atgtgtaata aattaaaaga gcaagacact aatacgttac
      361 acttatcctt tgatgagcta aaagaattaa gtaattataa tccagaaacg cgtaatataa
      421 acagatttgt agatgactta caaagagttt acgataaaat gctagatatt cgatatacta
      481 ttagaacaga aacgaaaata aagaaatttg ttctgtttta taaatatgaa attgatatat
      541 ctgaaagata tttagaaatt gctacttctc cagatttaaa atatattcta aactccatta
      601 ctagtaattt tactaaattt gaattaaaag aaatgacaca tctcaaatct acttacgcca
      661 aaaatatgtt tagaatactt aaacaataca aacacactgg ttatatgaaa attaaaattg
      721 aagattttag agaacgttta gatattccaa attcttatag aatgtctaat attaatcaat
      781 ttgttctaaa tccaatcata aaagaactat ctacgatatt taaaaatctt catattaata
      841 aaattaaagc gaaaaaaggg cgtaaaattg agtggttaga gtttacattt gatgctgaga
      901 aacgtattca tagtaaacga cagccacaaa tggcgaatat aggtaagtca cgacagatga
      961 caagtcgtga gaaaacgcca aaatggttag aagaacgaga taatccgaaa tatcagaata
     1021 aatataacgt tgacgaaaat ttcgaaaaag ataaagaagc ttttttaaag caacttaaag
     1081 tggattggga agaataaagt aaaaaaagta attggtttat atatttttaa tgttagtatt
     1141 ataaatacat atacatatta aatttatttg aaattaaaag gagataataa atgaaaataa
     1201 ctaaattttt tactttattc ttactttttt tgacaattac aattgttttg agtggatgta
     1261 ataaagatgt tgaacacaaa aaaaatatat ctattaaaga tatcattaat gataaaaaac
     1321 agcacatgtt cttttcaatg tctactgatg aagataataa taacgagatt gaatctatat
     1381 acttaactaa aaatggtcaa atgaaggaga tctgtttggg ttcttcagaa atatctcctt
     1441 catttctact gaatttagat cctaatgaag ttaaaaaagt ccttgataat aaaaagattg
     1501 attatcaaga aacaccctat aaagatgtta aaacttattt agaagtaaca aatgaaaaag
     1561 ggaatgccct tattacattt gttaccacta agtatatttc tgaaaagaaa gctaaaaaag
     1621 gtctaggcga tgtactcaaa tcagtctatt caaatgataa aaaaaccgaa ggcttattac
     1681 tttatacaaa tgtgagtaca ggaagtatac aaagtgctgg tgatgctggt aagtatactg
     1741 gccaattaac caacgataat ttacctagtt taatgaatga atctaatgat gaatctgaca
     1801 gacttgatac aattattaaa ttaaataata aagatcaaaa aatggtgaat gtttcttctc
     1861 aagataagga tgtcatcaaa attaaattat ctgattttga tacgaaatta gactaaaatt
     1921 aaatttctat tacttcaaca ttttgtattt tttagacatc ataattttac aaatctcttc
     1981 atgcaagata tactgaagtt aacaaatatt aagtgcatca aaacaccgac cccccaatct
     2041 actgcaatag attggggggt cttttagtgt tgactatgtc gtcttatttt ttattacgtt
     2101 gttctagcca aaatttaaac aacgtaacac tgcactctac tataataggt gctattataa
     2161 aaacaaatat taagtacatc attacacctc cttccgtcga atctatcgcc ggaagtaaga
     2221 cgacttcaat agtataacag aatctgattt taatagattc tccgtttttg aataaaaata
     2281 caaaagttca tttaatgaaa aagaatgaaa acgcgtcgtt aaagggatgt ataaaatttg
     2341 tataaagagg aatgatgaca aatagggaca aacgctgaaa tgacggcgtt tgtccctttg
     2401 tgttgagttg acataattaa ctattatggg tagtgccttt gtataaatac tcaaaaacac
     2461 aaacattgtt aaattaatct ttgtgtcctt taatataagt aacataatag ttaattattg
     2521 cgatgagaaa aaccatagat aactaaatat ataagcataa taactatgaa atgaatagct
     2581 cctaaaaata atgcgaaaat atctgtagta agaggtttaa aataatctga tgaatcgtaa
     2641 atattcataa accaatgaac acattcccaa gaaagaaatg ttccaataca tctacttatt
     2701 ataatactta tattattttt taagccgaat ttaaaaatag atctacttat taatatagca
     2761 actattataa atgggatgta acctattaat aaacctgttt gataatcagt aaataatccg
     2821 ataaattcta ttggaactaa agataaaatt aatataatga tttttaacca tttcatttta
     2881 aaatttctct tttcaatatt atttttcctt caatatcatt ttattaatat aattaggtaa
     2941 aagtctacgc tgtctttaag tgatagatga atgatctcta ttttttatta tgttatacat
     3001 ttatattatt ttctgtaagg ttctgattct aaaagatatt tataatagga gtctttcttt
     3061 tgtaaatctg tttcccccca taccttatat tctttatcat ctgtccatgg tgctggtgct
     3121 ggtgtcacgt tatactcata ctcactatat ggttgatctt taaaagcaat atctttttca
     3181 gcatatccca tcataatatt aaaactcatt ttttcatttt ttattttttt atcccaaccc
     3241 ttacgttcta taacgtgatc gatgattttc tcactcttgt gttcctgata agtaatcaca
     3301 ctaaaagtcc aaactgttaa ggtaataatg ataacaataa tagaagttat taatattttt
     3361 aagattttct tcatttttgt ataattcctc ttttttaata atttttatga taaatatgta
     3421 ttattgtatt taattttaca aaatattaat taaataaatt tacttaagga gactaatatg
     3481 aaaagaaaat ataaaattac aggtatcatc tttatcgttc ttcttatcgt tttaacatta
     3541 atttttagta tagtgcacca ttatgctaat gttcaaaaac atgaagaagc taaactaaaa
     3601 caaaaagttc accatatttt caaacaaaaa ggttgggaag ataaagttaa agaagaaaaa
     3661 aatatattta cgttcaatac tggagataat gatttacaag tcacttttaa agatgagcct
     3721 tataatacgt atacatattc tattgatgaa gacaataaag tatatggaaa tgctgttttg
     3781 aaagatgaat atgataaaga ttttgatagt aaaaaaaagt acaaagaata tttaagaaaa
     3841 atgcattttg aagaaaaata tgatctgaaa taaaaaagca agcgactacg ataattgata
     3901 gtcgcttgct ttttagtgtt ctttcaattt gtttaatgct tttttatgat tcgttttcta
     3961 tcttgtaatt tggaggctta tatatgccat tttaaaagca tatgcgcgtc tttaatatga
     4021 aatatactaa aattacctat atgatattaa atgaaaagcc tgtataaagt ttaaaatagc
     4081 ttagaaagca aaaaggagta ttagcattga aatcaattgt tcattttatt tgcctcttta
     4141 tcatattttg tatttttgga tatgtgatag atttaatatt ttttaaagaa ataaatattt
     4201 ttctgatttt agaatcagcc tttattttat cattactaat catgccgata ttaaagattg
     4261 taaaagagat agaaattgaa aaatagaact ggtaaatacc attttctata cttaatgttt
     4321 aataaaaata ggctactaaa attcagttaa taaagattca tctttattaa ctgaatttta
     4381 aaaaaatata tatagtgtta atttttttaa ttaaagtagt tataacttaa tttatatgat
     4441 aaagtcatat ttaaacatat aaatatttta caggaggtct ttttatgaaa aagttacttt
     4501 cagtttttat tgcaagttta ttagttttaa ccggattatc tttttcttca attaatcaag
     4561 gtaatactgc gagtgctaaa acaaaatata aaacaactat aacttacaaa ggtcaaaaat
     4621 atgtatatgt tggtcattat aaacaccatt tttctaaaaa agaagttaaa aaagtagtta
     4681 aattttctaa aggagttcat tcaggaaaca aattagtatc tgctgctagt aaacttagta
     4741 aaaatggtta tgttaaagca tcctcggcta tttataaagc ctttgatttt ggattgaaaa
     4801 atgaattaaa aggtagctat ttttacacag cagctaaaaa aggtacaggc gtaacaattt
     4861 cttatgatat gtatactgca acaacaggtc cgaaccatac tatagctaag aattttaaac
     4921 atagttataa ttaaataaat gtgaggggaa ggattaatcc ttcccctcac atttattttc
     4981 aagctttttt gaacggttga attttagacc ataatttatg aattcataga tattagataa
     5041 tatttcagct attattctta aaatattttc cattatcatc actactttca attttattca
     5101 gtatatttaa tttttctctc gaattttttt atatccccat ttttatattt aacttcataa
     5161 agaatacttt ctcctaataa atcttttaca ttgtcgtatt tgtttgctac acctttccaa
     5221 atcaatgtat ctttagtatc ttttttaata ccgtcattag taatttcttt tgcattgtta
     5281 tttttatatc gatacacttt cccaattttt tcaggtttgt tattttcttt tttatttatt
     5341 gtaatattaa cagctttact tgtaggatta atttctaatg cacttttttt aattggtttt
     5401 tctttagaac tattactaca tccagtaaca gttagaacta aaataagaga aataataata
     5461 ctaaatattg cttttttcat tctaatcatc cttttttgct tattcaagat taccatattc
     5521 ttcatttaaa tattttgata ttaaattttc aatttgttta gatttattaa taccatattt
     5581 tgttttaact ttatctaaat tagttattaa tttaggagga agagtaatat ttaacttttg
     5641 tctttgttct ctttcattca aacccacaac actctccttt tatttatgcg tataaagtag
     5701 cattacatgc gtacaaagtc aagtttgtac ccataaaaga agtatagtat agtttgtttt
     5761 ttactttatg gagctattat aattacgaca aacgataaaa gaaaaacaat tttttcctct
     5821 caaaaaagcc ctatagtttt caactaagaa taaatgagca aagcaaatgg taagatagtc
     5881 cttcagctat gctgaatact accttaagcg gtaatggatt tccttatgct ctttaaaata
     5941 caaaagttac attatatacc tactttttca ggttttagct tttttctaag aagaacaatt
     6001 gagataaaaa atatattttc ctttctgaaa gtgttgagaa agaaatttta aaatgattta
     6061 tagcctatct gttgaacaaa aaagttttta gtcatttttt aataaaatgt tctagaaagg
     6121 gcttaaaatg caaaataaaa agactactca aaaaatgagt agtctgaaga atagtttttg
     6181 atgtattaaa tgaaagggag tactacatta atcatatcac acgcatttat accgagaaaa
     6241 tttattgatg ttgagaagaa cccttaacta aacttgaagc cgaatgtcgg catagcgtaa
     6301 gctattaagc cgacgattcg ataagtttag ggattgttaa agattcggag gttcaacgtc
     6361 aataaagcaa tttaaaacta tagttaaaat agggttctgt tgcaaagtca gaaagataag
     6421 tatataacct tatgtttctg actttgcaac agaaccactt taatttacca taaaaactta
     6481 aatattttga ggagataagg agtctttaaa tgaaaaaaat agtttatata cttggtacta
     6541 ttatagtttt aggcttttgt tatttttatg taaatttaat tttttttgat agttggacac
     6601 gttattctgc tgaaaaagaa ataaataatt atatttcaaa tcatgataca aagaaactaa
     6661 aagaaatatc cgcgaataat aaaacatatc aacttttgaa acatgctaaa catgtgacaa
     6721 taaaaggaga tacagataat caaggtggag gtcatatagg gtattttact agtacgatta
     6781 atggtaaaga aggagcgctt tttgtacatc ttaattttgg tttgtttcct gaaattccta
     6841 aagtaaccaa gttggaagtt tttgataaaa atatttatga gtagcataat tgctactcat
     6901 ttttcgtgtt agttattctg gttctgttgc aaagttagaa aaatatagct aattacgtcc
     6961 atatagcatt aagcttctat gctatgataa tcgttagaaa tctggctaag aatctggagt
     7021 gtttttatga tttttttaag agaaaaaata gtcatgaaat atacaaaaaa atcatgtata
     7081 ttttgttaaa tagtacagtg atagcattta tattattgct aattgtgaaa ttaatatttg
     7141 atttcctttt ttagactata ttcatatttt ttcagatttg tatttttatt ggggttaatt
     7201 attggtagta tcccagattt tatagaaaag aaaggttaat tttatgttat taaaaaataa
     7261 gatttattca ggcattatta ttgttatttt tatagtttgt ttgattatag gattgaatga
     7321 aaatatcatt ggaaatcctt taggagaaaa aatattttat ttattaggtt ttctattttt
     7381 tgtattatta attattggta caacaaaaaa tagaaagcat taatgtcttc tttttgcccc
     7441 atttcttacg ataaacattt tttgagtagc ctttttaatg ttctttcaat cgtttaattt
     7501 ctttttttaa tggtctatca tcacgatacg tattaaaatt cataaatgca gtactatcaa
     7561 agtgctctaa caagcgatga aatatctcat tctccctttt gtattgttct atatcatctt
     7621 caagttggtc atgacgttgt cgaagtgcgt ctctgtctga tttgagtgct tcgttgtctt
     7681 cttctagatg ttcaataact ttatacaaat tattcgtatt attcttagaa ttagaatcat
     7741 ccatcgttat ttgctctttt ggatattcaa tcaccgtcgg tttaatccga ttacccacag
     7801 ctgtttggcg tatatattct gtgacattca tactacgtat gtaggctaaa tcttgtatct
     7861 ttttgtattg cgcttgactg acacgaacgg ttaagatttt ttctttcgag ctttgttttt
     7921 ttggcattga atcacctcat aattaatata tgataagcat acatatgtgt gcttattttt
     7981 atattaatat aacatacata tgtatgttaa taaatatgta tgattcgagg tgttgtgagc
     8041 taacatttgg catacatatt atcaaacata tttgtttgat aatacgtatg tcatttgtta
     8101 gctatataac ggaattcaaa aatatatttt tgaatgtagt gatggcctcg ccagagcgaa
     8161 gataactctt acatttgaat gtaagagtat atcgagctat actctcattg cggaatcagc
     8221 ctcacatgtt tgaggcgtag cggtgagagt atccatcatt ttacataagc gaagcattgt
     8281 cgaaggtaga caatcacagc gcttgtaaaa tgatatttgc gaaggttctc gaaagtagag
     8341 aaccacagac aaatcaaaaa gcttcgggga gggcgctcaa tttgaaatta gcttggaata
     8401 cgcaaaaaaa taaaatgagt gatgttaaag gcaaagagat ggaacaagaa cgtaaaggga
     8461 gaatcaaaaa cgatgaaatt gacttgaacc ggacaaactt aaactttgat ttagttgaag
     8521 acgaacgtca tttgtaccat cgtgtgaaag atagaattga gtactataaa aaacagggct
     8581 cacgtgtaca gaaaaatagt gtggtgatgt attccaatat cattacgtta tcgaaagaag
     8641 aggccgaccg tatgggagag acgcgtacaa aacattactt taaaacatgt aaagattact
     8701 tcagtgaacg ttttggcgaa gccaatgtcg tttcagcgaa agttcatatg gatgaatcag
     8761 cgcctcatat gcaccttcac tttatacctg ttaatcatca agggcggtta tcagcacgta
     8821 cagctatgaa tcgacaagcc attaatcaca ttcatgatga attaacaaca catctgtgcc
     8881 agcaaggttt tgatgttgaa cgtgggagta ccgatgataa taaaacgtat attcaagata
     8941 ttcatgaata taaaaagaaa gcgaaatttt taagtgaatt agaccaacaa attgaaacga
     9001 aaaaacaatt gttgagggat atcaacgatt tacacgatga aaaagaaaat ttaactcggg
     9061 atattcaaaa cttaaaacgg aaaaatcatt ttttacaaga ggattctcaa cagactcaag
     9121 aagatatcga cctatacaag caactgagaa cgatttctag ggacgatttt aaaaaagatg
     9181 aggaaaagta ccgaaaagaa atcgaagcgt atgaggcgtc tctgagtggc cttagaatga
     9241 attaccgaga tttggacaag cgcaatcaac atttaacaac acaaaataat caactcgaac
     9301 accatttaga catgttaaat gcacgtcatc aagcgatgaa cgacatggta agaaatgata
     9361 cacgtatttt gtttgcggga cttagatgtg cattgaaaga tatgaacatt gaattaagcg
     9421 cagaaattaa atatcaacgt atgccagaag tcggtattga tcccgatacg gtggatcagg
     9481 gggctttaac acatgcatat caaggtgatg tgtcagggat agaccatcat ataagaaagg
     9541 catcagcacc tattaaaaaa gagacagaga atgacttaga attagaataa atcaataaaa
     9601 ataagcactc aaacaagtat gatactcgcc tgagtgctta tctcatgaaa agaggagata
     9661 aaaatttaat aagtatgatc aacctattaa gaacaatcat taccttactt acagcgttta
     9721 ttaaatagtt ctgtaaggag gaacaatacc tttgcatttc tatattatta taagaattcc
     9781 aaggtagcaa agatattgta ttggaagtga tagatgtgag tatgatttct aaattaattg
     9841 atgaagatat tttgggtttc taaactttaa aaaatatctc cattctttta aaagtttatt
     9901 tttgtgttga tttctttata aatatatttg aatgcgtttg aagtatttca ctccgacctt
     9961 tttaatatat ttttataaag ctttttaaaa cctttttaag tactgtttaa gacctagagg
    10021 gataaggctt
//
