LOCUS       NZ_CP129354             3123 bp    DNA     circular CON 24-DEC-2023
DEFINITION  Staphylococcus aureus strain Dog102 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP129354
VERSION     NZ_CP129354.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33696794
            Assembly: GCF_034042875.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Canine Staphylococcaceae circulating in a Kenyan animal shelter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
  REMARK    Annotation added by database at submitter's request
REFERENCE   3  (bases 1 to 3123)
  AUTHORS   Akarsu,H., Liljander,A., Lacasta,A., Ssajjakambwe,P., Brodard,I.,
            Cherbuin,J., Kuhnert,P., Perreten,V., Torres Puig,S., Labroussaa,F.
            and Jores,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2023) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2022
            Assembly Method                   :: Flye v. v2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 294x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/24/2023 14:35:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,831
            CDSs (total)                      :: 2,749
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 45 of 87
            Pseudo Genes (incomplete)         :: 36 of 87
            Pseudo Genes (internal stop)      :: 21 of 87
            Pseudo Genes (multiple problems)  :: 15 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="Dog102"
                     /isolation_source="nasal swab"
                     /host="Canis lupus familiaris"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed2"
                     /geo_loc_name="Kenya:Nairobi"
                     /lat_lon="1.3345 S 36.7331 E"
                     /collection_date="2015-06-27"
                     /collected_by="Joerg Jores, Anna Lacasta, Anne Liljander,
                     Paul Ssajjakambwe (International Livestock Research
                     Institute, ILRI)"
     gene            complement(78..1073)
                     /locus_tag="P3U57_RS14145"
                     /old_locus_tag="P3U57_14145"
     CDS             complement(78..1073)
                     /locus_tag="P3U57_RS14145"
                     /old_locus_tag="P3U57_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            1525..2106
                     /locus_tag="P3U57_RS14150"
                     /old_locus_tag="P3U57_14150"
     CDS             1525..2106
                     /locus_tag="P3U57_RS14150"
                     /old_locus_tag="P3U57_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            complement(2432..2620)
                     /locus_tag="P3U57_RS14155"
                     /old_locus_tag="P3U57_14155"
     CDS             complement(2432..2620)
                     /locus_tag="P3U57_RS14155"
                     /old_locus_tag="P3U57_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          complement(2970..3123)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="93.0"
                     /evalue="3.15e-75"
     Typing          complement(79..1073)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cactacatac agcatttgtc acaaaaataa gacagtactt ttttataaaa aatcaacaaa
       61 aaagacatta gtcaacacta atgtcttaaa taataatttt gcttttcgaa attccaaatt
      121 gcggtaattg aatgtgcttt ttcttcttcg tctgttgttt tatcttcatc acttgcatta
      181 atcaaattac catcttcgac atcgtctaag tttaaaattt tatgtttttg tttaagcaat
      241 ccaccataac ttaacatacg ttttcgatac aaaccttttt ctaaatcact tacaatttct
      301 tgattttttt catcatcatc agttaaaaaa tcagatgatt taaccgaata ttttgaggtc
      361 tctttgattg ccgattcaat gtctttatcg ccttttctat tcggtttgat cgctttaaca
      421 ttagcaacag gtcgatagtc aacttgtaat gctctttgcc ataaattaac ccattcttct
      481 tgagttatat aattctcttt ttttctaaaa tatgcatttt caacacataa caaaacatgc
      541 atgtgctgat tataactacc gtcattttta ttaacggtaa cttctgttga acgcataaat
      601 ccaacaagat tttgtttaac ctttttatat ctactcaacc tatcaaatgc tttagttaga
      661 tgcttcaaac tttgttctaa agtatctcca tctatcgcat tttttgttga aagtgttaaa
      721 aacaaccaac gtgcttttgg cttttcctta attacttttt caatcacttt ttgagcttga
      781 taactatttt tcatagcacg tctccaatta caaaccggac ataattttga tttacaaaac
      841 catgtcttat gtaacttcaa atagccttca tcagttggct taaattctaa aatattacca
      901 cattgtttta cattaaaagc ctttttgatt tttaaaattt ctagtatatc tgcatagctt
      961 acattatcta ttttcttttc tctccatggg cgttgtttcc cacttttcgt catatctttt
     1021 aacgttttat tatcttgatt ttcggttata cttctagtag tattatattg catataaaaa
     1081 gacccccagt attatttaag attcgacacc ttaataatat aactggggtt ttttattgtc
     1141 aaattttcct ttcacgcaca aaaacacgtt taaacactgt aatatcaaca gttacagcta
     1201 tatatataaa atcgagttat ttctatatag tatcaagaca agaagaaact cgttcacact
     1261 cgtttcaaaa aacaacctat tccttcttct ttttatgacc ctctaaacct aaataaatta
     1321 atgtgaaaac caatgaataa ataaaacttt ctaaaaagtc aaaaggatta tcaaataaaa
     1381 tcgaattaac aaaattaaaa attaaaaata cagataataa aataatcaaa aagtaaaaaa
     1441 acttattcat attaaactcc tttgttttta tatgttaata ttatattgaa ttcatttaaa
     1501 taacaaaaca agggggcatt tacaatgaat gcaattcaaa acagtatatc actaagaaag
     1561 attttagtag tatctttaag tattttaacc atactttcaa tggtcttgga ttttaatttc
     1621 aaagaagctc aagcccaaaa caaaaataac attgcagata aaaacgttga aactttaaac
     1681 gaaaaagaaa ttgaaaaaga actaaaatac atatatggca aaattattat tttagataaa
     1741 gacggaacag ctaaagatgt taatctagaa aatgccaaat ctcgatatgg atatgttcct
     1801 gaaggattcc aaaaactaaa aaacgacata gagaataaaa agaaaactag ttcaatatca
     1861 cctagagcag ttggtgggaa ctacaaaaat agcggtgact gtttctattc tgaagtttta
     1921 aactcatatg gagaactttt aactggaaac ataatcacag cagtttttga agatgttaaa
     1981 gccaaaaact taaagggcgt agcaaaaaaa ttagctaaaa ttgggataaa aggaaaccta
     2041 gctggaatcg cagctactat ggtttcgaag gatgtacaat gtaatcttaa atacgggtta
     2101 ctttagatac tatttattcc aattgcttta ttgacgttga gcctcggaac ccttaacaat
     2161 cccaaaactt gtcgcatggt cggcttaata gctcacgcta tgccgacatt cgtctgcaag
     2221 tttagttaag ggttcttctc aacatcaata aattttctcg gcataaatgc gtcgtctatt
     2281 ttttattatc ttcatgataa tcatattttt cttccatctc gtcaaaagca tcatctttat
     2341 agaaaaacaa aaagtaagca acaacaccaa taggaataaa aaaacagtgt gaaaacaaga
     2401 agaaactcgt ttcaaaatcc cctcaaatat tctaattatt ctttttgaaa ttcataacct
     2461 taacaaccaa ataaatagca tttattccca tcaaaataac atacggaatt aatatatata
     2521 aattttccaa atgagatata aaaattaatc caagtaaaaa gaaaacattt aaaatagttg
     2581 ctataacgta accttcatct aacactgtgt ttttattcaa aataaacact ccttttttaa
     2641 aaaactttat cacaagactt actcaaatta taaatacctt tattttgagt tttaaggggg
     2701 tattttaaag gtttaggggg gtatcatata ggtttttaac ttaaaaatat atacaagctc
     2761 ttaaaacgca aatatgagcc aaataaatat atggtaattt cacaaacaaa aatttcagca
     2821 aaaccagtga ctaatttttt aacactgccc atttacatgc aaattaaaaa ttagcataaa
     2881 atcctttaaa acttccacat gtttttctaa attcctgcag ggcgtttttt atcaaaaatc
     2941 gagaattttt aatttttctc taaaaactcc gtaagagcat aaggagaatt tgctatcatt
     3001 caaaaatgat aggtctggca aagccagaca ttccaggggt tttaaggtat tttaaagtga
     3061 tatcatacaa agctgtactg tcttattttt gtgacacaat atattgcgtt tgtcttattt
     3121 ttg
//
