LOCUS       NZ_CP129351             7769 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain STEC435 plasmid p_STEC435_3, complete
            sequence.
ACCESSION   NZ_CP129351
VERSION     NZ_CP129351.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36269922
            Assembly: GCF_030438455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Prevalence and Characterization of Shiga toxin-producing Escherichia
            coli isolated from asymptomatic carriers in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, NO.155 Changbai Road, Changping District, Beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:27:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,957
            CDSs (total)                      :: 4,838
            Genes (coding)                    :: 4,665
            CDSs (with protein)               :: 4,665
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 78 of 173
            Pseudo Genes (incomplete)         :: 81 of 173
            Pseudo Genes (internal stop)      :: 49 of 173
            Pseudo Genes (multiple problems)  :: 35 of 173
            Pseudo Genes (short protein)      :: 5 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129351.1.
            Bacteria and source DNA available from China CDC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC435"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p_STEC435_3"
                     /geo_loc_name="China: Qinghai"
                     /collection_date="2013"
     gene            complement(join(6749..7769,1..452))
                     /locus_tag="QYQ94_RS24720"
                     /old_locus_tag="QYQ94_24720"
     CDS             complement(join(6749..7769,1..452))
                     /locus_tag="QYQ94_RS24720"
                     /old_locus_tag="QYQ94_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_086259340.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDDLEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            697..987
                     /locus_tag="QYQ94_RS24725"
                     /old_locus_tag="QYQ94_24725"
     CDS             697..987
                     /locus_tag="QYQ94_RS24725"
                     /old_locus_tag="QYQ94_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063106837.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVATGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNDKKVMEKTGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(979..1257)
                     /locus_tag="QYQ94_RS24730"
                     /old_locus_tag="QYQ94_24730"
     CDS             complement(979..1257)
                     /locus_tag="QYQ94_RS24730"
                     /old_locus_tag="QYQ94_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063106834.1"
                     /translation="MKKCVPVLLFSVLSVSVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            1379..1801
                     /locus_tag="QYQ94_RS24735"
                     /old_locus_tag="QYQ94_24735"
     CDS             1379..1801
                     /locus_tag="QYQ94_RS24735"
                     /old_locus_tag="QYQ94_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032319274.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1860..2126)
                     /locus_tag="QYQ94_RS24740"
                     /old_locus_tag="QYQ94_24740"
     CDS             complement(1860..2126)
                     /locus_tag="QYQ94_RS24740"
                     /old_locus_tag="QYQ94_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262325.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGR
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2110..2334)
                     /locus_tag="QYQ94_RS24745"
                     /old_locus_tag="QYQ94_24745"
     CDS             complement(2110..2334)
                     /locus_tag="QYQ94_RS24745"
                     /old_locus_tag="QYQ94_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879770.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2488..2700
                     /locus_tag="QYQ94_RS24750"
                     /old_locus_tag="QYQ94_24750"
     CDS             2488..2700
                     /locus_tag="QYQ94_RS24750"
                     /old_locus_tag="QYQ94_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000350688.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLDK
                     ENGLLICRPVQKEINGTWYTVDLSK"
                     /name="hypothetical protein"
     gene            complement(3232..3465)
                     /locus_tag="QYQ94_RS24755"
                     /old_locus_tag="QYQ94_24755"
     CDS             complement(3232..3465)
                     /locus_tag="QYQ94_RS24755"
                     /old_locus_tag="QYQ94_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3472..4032)
                     /locus_tag="QYQ94_RS24760"
                     /old_locus_tag="QYQ94_24760"
     CDS             complement(3472..4032)
                     /locus_tag="QYQ94_RS24760"
                     /old_locus_tag="QYQ94_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_074167501.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4064..4501)
                     /locus_tag="QYQ94_RS24765"
                     /old_locus_tag="QYQ94_24765"
                     /pseudo=""
     CDS             complement(<4064..4501)
                     /locus_tag="QYQ94_RS24765"
                     /old_locus_tag="QYQ94_24765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4710..5033)
                     /locus_tag="QYQ94_RS24770"
                     /old_locus_tag="QYQ94_24770"
     CDS             complement(4710..5033)
                     /locus_tag="QYQ94_RS24770"
                     /old_locus_tag="QYQ94_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5101..5292
                     /locus_tag="QYQ94_RS24775"
                     /old_locus_tag="QYQ94_24775"
     CDS             5101..5292
                     /locus_tag="QYQ94_RS24775"
                     /old_locus_tag="QYQ94_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6301..6432)
                     /locus_tag="QYQ94_RS24780"
                     /old_locus_tag="QYQ94_24780"
     CDS             complement(6301..6432)
                     /locus_tag="QYQ94_RS24780"
                     /old_locus_tag="QYQ94_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            6462..6752
                     /gene="cki"
                     /locus_tag="QYQ94_RS24785"
                     /old_locus_tag="QYQ94_24785"
     CDS             6462..6752
                     /gene="cki"
                     /locus_tag="QYQ94_RS24785"
                     /old_locus_tag="QYQ94_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_074167500.1"
                     /translation="MHLKYYLHNLPESLTPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     Typing          complement(5379..5466)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          5489..6035
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.374"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3126..4720)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5093..5295)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.176"
                     /coverage="100.0"
                     /evalue="5.47e-12"
ORIGIN
        1 tcttcacggg ctttttgctc tgccttcgca aggcgcaaac gctcagcctc tgcctgcatt
       61 gccatattat tggcatgagc aaggtcagtt actgatggac tacgggcggc attagcacgt
      121 aaagcgtcat taacaatatc cttgagacgt tgagtcaggg catcacgctg agatttcgct
      181 tttgccaatg ctgcctcagc cgcagcagca cgggcagcct gttcagcctg atgttttttc
      241 aactgcgcgg tagaccattt agcggttgca tgtatcgcag cagaactttc actggcccca
      301 ccaaatgctc caccgcctcc accaacattt tcagttccag ttgcaagcgt cgctgtaatc
      361 gtatcgccag tatcaacccc aacagttggg aatgacccct cagtagattc tgtgctctcc
      421 acatctggag aattatcagt gactttatcc ataaagaaat tcctctttga taattaaaac
      481 aataaattaa aaacaacata ctgtacatat aaccactggt tttatgtaca gtgaaaacct
      541 actactcagc attgtccatg tcaagagcat ggattttcat ttttgcaata aggatcacac
      601 tatggggagg caggcattga gaacgtcgaa acagaacacc ggagcaaatc aggatgagat
      661 ataaaactgt tggatcatga aaaaacggag aacgatgtga gcaaatcacc ccgccataaa
      721 ctgaacaaaa cagacaaacg acttctcgac acccttgttg ctaccggata tgagcatgac
      781 aaagcccgtg acctcatcca gaaacaggtt tacacgctga cactggcaga tcagcgtcat
      841 gtggtcagtg aaatcagtaa tggtgtgaat cccacccagg cttactcggc ggtataccag
      901 gcaagacgca ttcgcctcgc ccgtaaatat ctgaacgata aaaaggttat ggaaaaaacc
      961 ggggaaaata cgcccccatc agcgtaagga tttcttttgc cgctccagag actccagttt
     1021 tttacgcaaa tcctctcttt tttgggcatc tctggtgcca gccagctctg ctctcaactc
     1081 atcgatctga agttgtatct tcagtctgtt actgaacatt ttctgtctgg cattaacatc
     1141 cgcaacaatg ccgttttttg tcttctcggc cttttgttga aaaacattgc tgtccgcatg
     1201 actggcaacc gaaacagaaa gaacactaaa aagcaggact ggcacacatt ttttcacggg
     1261 attattcctg actcattgac catcaaatca cattgggagt aaaccgacgt atgataagag
     1321 atactcttcg gagatataac tccctgagta tcaagattaa aaacgcaagg agatgtttat
     1381 gagatctgcc gctgccaggc tgcttctgat acctctgata acagcaacaa tagctcttac
     1441 aggatgcaca ccaaagacca gcctggaacg acatacccgg cattatgttt atgcttcaga
     1501 tgatggattt gatcctaact tctacaccca gaaagcagac accatacgta tgatgctccc
     1561 gttctttcag cagttccggg atatggggat gaaagacaaa gcagccggag tatcagcaga
     1621 aacggcacag caacgtgtaa aagaattcca ctcagaaaaa ttttttcact cactccagag
     1681 cacaacaacc tttgctggca gaaaatacac aaacagcgat atgccttcgc cgaaaaaaat
     1741 gaaactaatg gcagacacca tttctgcggt ttatctcgat ggatacgagg gcagacagta
     1801 agggatttat cataatccct tattgtacgc cacatatgaa aaagcccgca tggcgggctt
     1861 cagtcgtaga cttcacgtcg atggccgatg gtggccacaa gaataatcag ttcgtcatcc
     1921 ctgatttcac acagaatgcg ataatcaccc acacgataac gccagaattc tcccaactcc
     1981 cccttcagtg gcctccctga tttacgggga tcggctgcaa ctgcaatacg taaatccata
     2041 aaatccacaa tccgtcgcgc gttctgcttg tccatcttac gtagcgattt gagcgcccga
     2101 tcggaataat taatcgtcca gaccaagaga cttccttacg tcctcagacg aatgcacagc
     2161 ctcatcacca cggcgaacac gtgctgctgt ttccgctgaa agataataat cttccagatc
     2221 ttccaggtgc gccagtatcg cttcccgggc atagaacgtc tttgtcctcc cggttttctt
     2281 cgccagcgag tccagacggg actcaatttc atcagacaac cgaatagcca gcatacaaat
     2341 ctccctccga acaattgtta tacatgtata gcaaaaatta taacgtaaaa agacgataaa
     2401 acaatcaaag gctggttgaa taaacagtaa aatcccatat attaacgact gatatatgat
     2461 aagtatcggt tttaaaagga aaacaacatg gatattgaaa tgaataacac tgaagagatg
     2521 ttcagcaaac tggggatgcg gttaggagct gagatggcaa tccctttgaa ggatggtgat
     2581 aattttgcgc caactggatg catcgttacg aagcttgata aagagaatgg ccttctgatc
     2641 tgccgaccag ttcagaagga aatcaacggc acgtggtaca ccgtcgattt aagtaagtaa
     2701 caccacggac taaagccctg cggggctttt ttatacctgc cgctgactct gattgaacat
     2761 aacatctgaa ttgtacgcac cgctgaaacg cgttcatcgc gatcacggca gcagacaggt
     2821 aaaaatggca acaaaccacc cgaaaagctg ccgcgatcgc acctgataaa ttttaaccgt
     2881 atgcatagct attcagccat gtgaataacg ctggttttgc ctgcgtaaac ctcatgacac
     2941 tgtttttttt ccatcttttc agttgatgac atacgcagac atcgcgggat gaggctgagg
     3001 aatgagcgcg atctggcaaa gaggcaaaac acagcaacaa aaacgacacg ccagaatcgc
     3061 gcccggatgc gtttttaacg cgttccggta ccatctggca acctcccgga acaactcacc
     3121 gtcacatccc cattgacggg ccacgccata cccgtacttc ccgttcctgc tcttcatgcc
     3181 aggaccgcgc acgctcccgt tccaggcgtg cctgcctttc ctgttcatcc cttatctgct
     3241 gttcgtgata aataaccgac tcaagcggtc cacctgcccg gctaatctct gcacctgctg
     3301 actcaagtcg tcgcactgtt ccctcagttg cccgttctcc tgtcgtgtca gctcgaacat
     3361 atgctgcaaa tccgtgaagg cgctctccca gtctttcagc cgctgcgtat agtcctgctg
     3421 caattgctct aaggcgttca gtaagtgcat ttccagctct gtcatactca cttactccct
     3481 gaccagtctt actgcgttct tcttctccac cgtccagttg ttttccccct tcaccccgga
     3541 cggcagcacc agaaatttcc cgttcctgcc ctcgtgatac gtcacacccc atgttttttc
     3601 ccggagtttc gccagcgtct cttcctggtc cctgatagcc aggatgttcg ccgcaatacg
     3661 gctttcctgc cactgaatca gcccccatga cgccagaaaa atcccgcccg tgacgcaaag
     3721 cgccgtcagc gacgggtaca gtatccgccc tttgatcagc ttccagagca gctcttcctg
     3781 ccgccgggcc agttcgttct ctgtggcgct gaactgcgcg ttcacggcac tgttcagcgt
     3841 ctccagttgt tctttcaccg ctgctgtgtg tgcgctgata gcgtctctga ttttctgccc
     3901 gtttaagttc agttccctgt ctacagacgc ttcgagcttc ctgaactcgc tgttcagcat
     3961 gttctctgta gagacggcac gctctttcag tttcttctcg aagtctgtcc ccatttgtaa
     4021 aagattgctc atacagcgcc cctttcagcc tgagattacg cccgccctcc gggtcggcga
     4081 tactgatact gctcctggtt gtcctcacca cctcaaaacc tgccgctgta agcgcctcag
     4141 tgacatcctg acgcgttttt agcgctccgg catggtaaag agcctccagt cccctcgtaa
     4201 tcgcttctgc ggcctcctgt ttcgctttcg gcagattatt cggggtgaca agtgtccgcc
     4261 tgttctccgg tgcgttcggg tcgtgcagcc cgtaatggtg attcaccagt gtctgccagg
     4321 cattgattcg cggacggtcc gcccggtcgt aatagggctg gagccgtttt ccgctcgcca
     4381 gctccatatt cgggatgaca aaattcagct caagacgccc cttgtcctgg tgctccaccc
     4441 acaggatgct gtactgattt ttttcaagac cgggcatcag tacccgctca aagctctcca
     4501 tcaccctttc acgctctccc ggtggcagtg tctgctctgc aaaagacaga acccccgagg
     4561 tgtatttttt cgcaaacggc gtcgcatcga tgagttcccg cacctcttcg ggagcacccc
     4621 gcagaactct cgccccttcc cggttacgct cccggcccag caggtaatca accggaccac
     4681 tgccaccgcc ttttcccctg gcatgaaact taactatcat cccgttctcc ctgtttacgg
     4741 acctcatccc tcagctcact cagttcacgt ccgatggcca tcagtgcagc caccacatga
     4801 acccggtcat gccccgacca ctgtccgctg tttatcttcc gggctatctg attcaggtta
     4861 ttgccgaccg aagcaaactg gcgcaacagc ggcggtgcca gtgtcggaag gcctgacgtt
     4921 ttcgatggcg gtgcccccag gcagacctta cgcatccatg acgcaagttg ttttccctca
     4981 caacgtgcca gcagccgcgc atgttcctca tccgtgaccc gtatcgtgag catcctttcg
     5041 cgtttcaccg gtatcattaa aaacctccga cagactcccc acacatggag aaacagaact
     5101 gtgactaaac aggaaaaaac cgcccttaac atggcccgct tcatcagaag ccagacgctg
     5161 accctgctgg aaaaactgaa tgaactggac gccgacgacc aggctgacat ctgcgaagcg
     5221 cttcacgacc acgccgacga gctttaccgc agctgcctcg cacgcttcgg ggatgacggt
     5281 gaaaacctct gacacatgca gctcccggag acgggcacag cttacctgtg agcggatgcc
     5341 gggagcagac aagcccgtca gggcgcgtca gcggtttttt agcgggtgtc ggggcgcagc
     5401 cctgacccag tcacgtagcg atagcggagt gtatactggc ttaatcgtgt ggcatcagtg
     5461 aggattgtat gaaaagtgca ccatgtacgg tgtgaaatgc cgcacagatg cgtaaggaga
     5521 acatgcagat gccgatgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5581 cggctgcggc gagcggtgtc tgctcacaca aaagcggcaa tactgttatc cacagaatca
     5641 ggggataacg ccggaaagaa catgtgagca acaagacaaa gccggggaaa aggccgcgca
     5701 ggtggcgctt tttccatagg ctccgccccc ctgacgagca tcacaaaaac cgacgctcaa
     5761 gtcagaggtg gcgaaacccg acaggactta aagataccag gcgtttcccc ccggaagctc
     5821 cctcgtgcgc tctcctgttc cgaccctgcc gattaccgga tacctgtccg cctttctccc
     5881 ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt
     5941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttaacgc cactggcagc
     6061 agccattggt aactggatag tggatttaga tacgcagaac tcttgaagtt gaagccttat
     6121 agcggctaca ctggaaggac agcatttggt atctgtgctc cactaaagcc agttacccgg
     6181 ttaagcagtc cccaactgac ttaaccttcg actaaaccgc ctccccaggc ggttttttcg
     6241 tttacaggca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6301 ctactgaacc gcgatccccg tcagttcaga agacgaagat ggtgcaacgg ttcctccttg
     6361 cacatcacga atatagtttg cttgacatcc tgacaagaat aatattgtaa ttagaaaaat
     6421 tattattttc atatttcttt ccaacaaaag taaaaatact tatgcattta aaatactacc
     6481 tacataattt acctgaatca cttacaccat ggattcttat tttaatattt aacgacaatg
     6541 ataacactcc tttgttattt atatttatat catcaataca tgtattgcta tatccatact
     6601 ctaaattaac catatctaga tatatcaaag aaaatacaaa gttaaaaaaa gaaccctggt
     6661 acttatgcaa gttatctgca ttgttttatt tattaatggc aatcccagta ggattgccaa
     6721 gtttcatata ttacactcta aagagaaatt aaatccctaa caactcatta agtttgttca
     6781 actcatcatc ccctatgtaa gaagaaacaa tacctgtgac aattgcaatc ccccagaaac
     6841 caagaggcac accaacaata aaactaaaca ttaatgccac aatttttgcc acacctacat
     6901 cgaccgcact tttctccagc gtgacaaaca aaggtcgcca gttaccagtt tctattgcat
     6961 ttttaaaatc agaaccaaca tcatatagaa aagatactcg actagtgatt ttaagcgact
     7021 ttgatatctt cgttaaattc ttagataact catcataatt aacagactct aacgcattaa
     7081 aaatagcatc ccgatcaacc ttactgattt tcttatccag tacattttta tacttttcaa
     7141 atgcagcaag agcttcatca acactactaa atttactacc cttcgccttt tcagctaaat
     7201 cctgagctat tctggaatat ttttccccat attgttcggt tatatattga taaaacccaa
     7261 ccatagtatc aacagagtct tttacctgac tcttctcaag atcatcctta gcttctttta
     7321 attttatctc agcattctga acatctgttt ttttagcatc tctaatgcta accgcagaat
     7381 taagagcatt cgtcactgac gaatattcat tcttcttatc ctgcaattgt ctctccagtt
     7441 ttttctgaac atcacgccat ccaggaaatc cagtaacctt cacatcaaca cttttttgag
     7501 cgttttcaag ctcccctgca actctggaaa caatcgcttg cttactctgt acatcgcttt
     7561 cggccttcgc cagttcagct ttagcctcag ccagcttctt ctctgccact tccacagccc
     7621 gatgctcttc atcaagagaa tcctgtcgat ttttctcagc ctctgctgcc tccgccattg
     7681 ctaacaaatg cgcggtttca gcctgttggc gggcaatctc atcacgttgg cgttctgctt
     7741 cccggagcgc tttttctgct gcttcagct
//
