LOCUS       NZ_CP129350            11140 bp    DNA     linear   CON 17-JUL-2023
DEFINITION  Escherichia coli strain STEC435 plasmid p_STEC435_2, complete
            sequence.
ACCESSION   NZ_CP129350
VERSION     NZ_CP129350.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36269922
            Assembly: GCF_030438455.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Prevalence and Characterization of Shiga toxin-producing Escherichia
            coli isolated from asymptomatic carriers in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, NO.155 Changbai Road, Changping District, Beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:27:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,957
            CDSs (total)                      :: 4,838
            Genes (coding)                    :: 4,665
            CDSs (with protein)               :: 4,665
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 78 of 173
            Pseudo Genes (incomplete)         :: 81 of 173
            Pseudo Genes (internal stop)      :: 49 of 173
            Pseudo Genes (multiple problems)  :: 35 of 173
            Pseudo Genes (short protein)      :: 5 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129350.1.
            Bacteria and source DNA available from China CDC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC435"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p_STEC435_2"
                     /geo_loc_name="China: Qinghai"
                     /collection_date="2013"
     gene            complement(<1..1471)
                     /gene="mobQ"
                     /locus_tag="QYQ94_RS24660"
                     /old_locus_tag="QYQ94_24660"
     CDS             complement(<1..1471)
                     /gene="mobQ"
                     /locus_tag="QYQ94_RS24660"
                     /old_locus_tag="QYQ94_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_301403369.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAH"
                     /name="mobQ"
     gene            1659..1934
                     /locus_tag="QYQ94_RS24665"
                     /old_locus_tag="QYQ94_24665"
     CDS             1659..1934
                     /locus_tag="QYQ94_RS24665"
                     /old_locus_tag="QYQ94_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(2151..3065)
                     /locus_tag="QYQ94_RS24670"
                     /old_locus_tag="QYQ94_24670"
     CDS             complement(2151..3065)
                     /locus_tag="QYQ94_RS24670"
                     /old_locus_tag="QYQ94_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277132.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(3594..3737)
                     /locus_tag="QYQ94_RS24675"
                     /old_locus_tag="QYQ94_24675"
     CDS             complement(3594..3737)
                     /locus_tag="QYQ94_RS24675"
                     /old_locus_tag="QYQ94_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(3799..4056)
                     /locus_tag="QYQ94_RS24680"
                     /old_locus_tag="QYQ94_24680"
     CDS             complement(3799..4056)
                     /locus_tag="QYQ94_RS24680"
                     /old_locus_tag="QYQ94_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(4059..5804)
                     /gene="col"
                     /locus_tag="QYQ94_RS24685"
                     /old_locus_tag="QYQ94_24685"
     CDS             complement(4059..5804)
                     /gene="col"
                     /locus_tag="QYQ94_RS24685"
                     /old_locus_tag="QYQ94_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_000012960.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMSGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSKQ
                     FNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHID
                     IHRGQ"
                     /name="col"
     gene            complement(6037..8256)
                     /gene="mobQ"
                     /locus_tag="QYQ94_RS24690"
                     /old_locus_tag="QYQ94_24690"
     CDS             complement(6037..8256)
                     /gene="mobQ"
                     /locus_tag="QYQ94_RS24690"
                     /old_locus_tag="QYQ94_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_301403370.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHRWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            8444..8719
                     /locus_tag="QYQ94_RS24695"
                     /old_locus_tag="QYQ94_24695"
     CDS             8444..8719
                     /locus_tag="QYQ94_RS24695"
                     /old_locus_tag="QYQ94_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(8936..9850)
                     /locus_tag="QYQ94_RS24700"
                     /old_locus_tag="QYQ94_24700"
     CDS             complement(8936..9850)
                     /locus_tag="QYQ94_RS24700"
                     /old_locus_tag="QYQ94_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277132.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(10379..10522)
                     /locus_tag="QYQ94_RS24705"
                     /old_locus_tag="QYQ94_24705"
     CDS             complement(10379..10522)
                     /locus_tag="QYQ94_RS24705"
                     /old_locus_tag="QYQ94_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(10584..10841)
                     /locus_tag="QYQ94_RS24710"
                     /old_locus_tag="QYQ94_24710"
     CDS             complement(10584..10841)
                     /locus_tag="QYQ94_RS24710"
                     /old_locus_tag="QYQ94_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(10844..>11140)
                     /locus_tag="QYQ94_RS24715"
                     /old_locus_tag="QYQ94_24715"
                     /pseudo=""
     CDS             complement(10844..>11140)
                     /locus_tag="QYQ94_RS24715"
                     /old_locus_tag="QYQ94_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /name="HNH endonuclease signature motif containing protein"
     BGC             1..10804
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1114..1472
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1473..1655
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.109"
                     /coverage="100.0"
                     /evalue="1.56e-73"
     Typing          7899..8257
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8258..8440
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.109"
                     /coverage="100.0"
                     /evalue="1.56e-73"
     Typing          complement(2152..3065)
                     /name="rep_cluster_2134"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8937..9850)
                     /name="rep_cluster_2134"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1471)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.571"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6041..8256)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.917"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgcgccag ctcatcaccc cataccgtga attctccggc tctgttactc agcatcatgt
       61 agcgggttat cctgcccgtt cccggctctc tggcacgccc cagctccgta ataaccccga
      121 ctaatgccgt ttcacggctc agacggtggt tatcatgacg tttcagccgc gcctcatgct
      181 cctgttcctt ccggcgcgct tccgcttcgg cttcctccct gcgccgctcc gcttcccgtg
      241 ctgacagttt ttctttcagc tctgccattc ccggcgcaat ccacgtattc cactgcgttt
      301 caaaggcctg acgacgggcg ggaatgtccc gttctttcac ggccctgtag gtgcggcgaa
      361 gagattcaat ctgtttcccg agtttccaga aacggagttg tcttttgcgg tattccggtt
      421 cagcccatgt catcaggggg atctctgaca gaagggacgc gacagggcgt gcttttttga
      481 gtccggtcat ctccttttcg agaatggcct gctctttcag cagcgcattc ccctcacgtt
      541 cgatgtcgtt caccttcccg ctcatcgcca ggaactccgg ggtcatctcc cacgcgttgc
      601 cgcattcctg cggcaacgtg gcaatgaatt cccgcagggt atccgctgag gcattcgcag
      661 cgggaatggt caggttaagg gcttccgctg cacgacgtgc ggcatcgctg agttcccgtt
      721 tccgcacgtt ctcctgtacc tggatgactg ccagccttgt gcgggaaacc tgtatttcca
      781 ggttaaggct gttaatcaga cggttctgct caccgcgttc tgtttccatt ccccgttttt
      841 ccatcgcaca ggcagccttt ccgaggtgaa ggccgggcgc tttctccagt ccctgacgct
      901 cgtatgaacg atggtctatc tcttcctggt agccggcgtt cgcaagcgct ctgttcgcat
      961 ggtcagccca tgatgcgcgc cacgtctcgg cgagtgcccg gtcgttccag tccctgacct
     1021 ttcctgcaaa tcccgtttcc ccgacagctc tcgtggtcag catgatgtgc gcatggggat
     1081 tggtccggtc catgtgatgg aacgccacat cggcaatcat gccccgactg acaaagtttt
     1141 cccggacaaa agcgagaacg gtctcccgtg cggcctcgcg ggaaatctca cggggaatgg
     1201 cgagttcaat ctcccttgcc agctgggagt tccggcgctg ttcggctttc tcgaccgcat
     1261 tccagagaac ggaacggtca ccacaccagg acggtgcatt cgcaggggtg agaatgaccg
     1321 catcatcaac gccgcgttta cgggtgtaat cgtgggttaa tccggtacgg tcatcgggta
     1381 tgacagagcc ggaacggtag gcagcagaag caacggcact gtagccgttt tttcgcgaaa
     1441 tgattttcat gctgagatga taaatcgcca tttctgccgt aatgcctttt tgccgttttc
     1501 ttttagcgtc gcagaccgca cacaatcgaa acgaagtgga gattgtataa gtgcgcactt
     1561 cgtgcacttc gtgatgtgcc gtgcattatg ccagttcagt tttgcaacta ccagtgacgc
     1621 gctaaaatgc gatttttcac cgcgcacgga gtcacgaaat gagcagaaca ctcgaacaga
     1681 agattgccga agctgaggcc agattacagc gcctgaaggc gaagagccga agcctggaca
     1741 cggcgcaaaa agtcattgtg ggggcagcca tgctggcaag ggtacggagg ccggaagaag
     1801 cgcaactgcg tgcgttcctg ctccagtttc tgcgaaagga agtcacaaga caggcggatg
     1861 tgaaccgact acaaccgctg attaacgagc tggagaagct acccagacca ccggcgaaac
     1921 cgcagaacca ctaagccccg gcgcgtaagt gccacatgca ccaccggacg cctttgcgct
     1981 tcctcgctca ttcgctcgct acgctcggac atgagactgc ggcgagcgct accacctcaa
     2041 tcatgcggaa agccacacca ttgcgggatt caacgaggga aatagcaaca aaccaagctg
     2101 ctcaaaaaga cgccaaaaag gcgctgttat ctgatatagg cttatttggt ctactctacg
     2161 agacctcgtt ttttcagttg gtaataccag gcgcgactga tccctaattt ctcccaaggt
     2221 ttcagcgtgc gtgctgatgt agctactgtg ccgcgcttag acttagaacc gcccttgcga
     2281 ccgcgtgtag cctgaatttc tggcgtgtgc gtatcagcca catactgtgc gaaagtttcc
     2341 ggcgtgaagt tcctgtgcgt gtatttcgca atactcttgc caatagcccg acattcagga
     2401 ggtgaaagcg gaacggggag cgatgcgttg tacatttcga cacgctgaat caccgcatca
     2461 agccattgtg agaatgccgg ccagccctga cgaatagccc ggtaagccca tttacggccc
     2521 ttttcgaaca gatagcagtt tcgccccagc ccgtaatttt tatcgacgct acggcgcgct
     2581 gaggcgctca aatcgagata atcagccagt tcgtcgagag tgtagggttc ctcgcgccat
     2641 tccacttcct gccattcagg gtggcacgga tttttgcaga tcaggccgct gtaattcaca
     2701 tccgcgccca gtttttcaca caacgcacgc tcaatagcgg cagcgtatct gagcgccgaa
     2761 gccgatgcat ccggcgcagt tctcacagga agggcgagcg cgtagagcag atgcgcgtgc
     2821 ccgttacggg gattttttac ggtgatgttc ggggccggac aattccggtc gctccagtcg
     2881 attgatgccc ccgtccggtc cacgtcaaag ggaagccagt acagtgcatg tggctggttc
     2941 tgctggatga aacgggcaag aatggcaatg tttttcggca gaatgcgaac gccgtacgcg
     3001 aaatcgttcg tacagtacgg tttgtgcggt aatttttccc tgaagcgttg aagtacggcg
     3061 ctcatgtgat acgatttctc tcggtacaca gtttaactaa ccgcgctttc ccatcgggaa
     3121 acgcggaacc cggcgccctg taatttcgtg atcaatcttg gcggtgagag cacgatttac
     3181 ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt cgatccttcc
     3241 cgaaaaaccg atccatttac cataactccc gttgtacgta ccccttaaaa caccctcaat
     3301 cgctttctga cgcaaaaaac acaaaaagac aaggaaacac cagggtaaac ataaaaaatg
     3361 cgtcagaatc gtttttagcg cgttgcataa ctatgcacga aagcgataca cgatcggttt
     3421 cgtgcgttct gaacaccgaa cggctcaaaa cgacaagttt tctccccctg gatggaaagt
     3481 tctgcacgag aattttagat cccatctccc acatggaatt tttgtgacat ttcaaaaaaa
     3541 agcgcatcag aatcagcctg acacgctttg tttttgtcaa agaggatttc gggttactgc
     3601 gttgctaatc cggtcacttc agctgttgat gacggagata cggtcccgcc ctgaacatcc
     3661 cggatatagt ttgcctgaca tgcagccaga ataatgactg caagaagcaa taaaataatc
     3721 cctgttattt ttttcataac gactccttgt tgtgaaagaa tgccagtcat tcatccttaa
     3781 acaactggca ttcatatttc agccctgttt aaatcctgac ttaccgttag cagctcgcca
     3841 ttctttaatc tctttaataa cagcttcagg gctaccatca ttattacctt ctgggtaata
     3901 aatcagatca gaaccactag gatgctcagt aacacttata aaatgctcga ggttatcatc
     3961 ctgttttttt tcatcacctt cacaattgat gatgtcttca ataatttttt tgaattcagt
     4021 ttcagtgtaa tcactaatgc tgtttttcag ttccattttt actgacctct gtgaatatca
     4081 atatggcgtt tcggcgtagt gatacggagg ttgtccatat cataaacacc accatcctga
     4141 ctgattggct tgtcgtgatg aagctcaaat gagcgacgcc caccgacagt atctttattc
     4201 ctagcccgtg gagctaatcc ttggctcaag cgttttttat tacctggatt aaattgctta
     4261 cttaactcag gatctttcga cacttcttcc cagaattttc tccgaaaatc gtcgaagttt
     4321 ttaaattctt tatcacgcaa cttatcagca atgcgatctg gaattggcgc tcctgaatct
     4381 ttacctgcat catccagcca tttatcacca actggtttac ctttacctgt cgccttccct
     4441 ggcttattcc gtttactctc cttatctaat ttatccttag cgtccttttc tttattttcc
     4501 ttctgtttgc ggcgctcctg cgcggcactt aatgcagcat cagcatctga cttctctttt
     4561 gcagcagcat caaatgcagc ctgcttatta tttacatccg tctgcgcccg ctgggcttta
     4621 agcccggcca tttgccacat tctgtgaccg ccagacattg ggtcatgggc aaatcgatca
     4681 aattgtttta tttcagctat tgcatcagca agagtcttat tcgctgcatc aagttcgctt
     4741 ttacgcgaat tataaacctg aacagcttta gcctgtcgct cctgatttct ggcaacatct
     4801 tcatttgcct gattcagctc tgcacgcgcg cgttcataat ttcgctcagc cgcttcaacc
     4861 ggatgcgtag catcccattc ctgctgacgg cgattttctt catcctgacg ttgttttacc
     4921 tggtcagggc taagaacatc actcactgaa acatatacag cattatgacc gctgtccttc
     4981 gggaatcgaa taactgcatc cctggtatta ccaccctgag taaatcctgc cgggcgaaca
     5041 tccttatcag tattatttgt aacacctggg cttaatgtct gtactgctgg cgtactgtta
     5101 ttaactgaaa tattcagaac aggtgcacct ggaattgatg ccgtaaaaac acccggacgt
     5161 tcggtaggtt ttgcatcaac caccggaaca ctcatcggaa cacctgaaac aaccgaaata
     5221 ttctgtcgtt cgtcttttac atcatcaaca acacgaacat ttacgtttac tgttgcctta
     5281 tcgagaggta atgaactgac aggtgattca gtaatatcat ctgcgggtaa tgacgtcaca
     5341 atctttgaca tcatattggg gtcatctttc gctatttgtg atggcaatac accatataaa
     5401 gccacccccc aaagaccaaa tttaaacggt cctttcaggg cagccataat atcagcaata
     5461 gctgccgata atgctcccgc tgaaatactg accgccagac cgccagctcc tggagtggaa
     5521 agtgccggaa aaccaaatgc cactggcgca gctactgctg acagattacc gcctgttccc
     5581 gagccaccac cggaattacc attccccccg ccattaccat gaccggaacc acctccccag
     5641 tgaatgccgc taccggaacc accaccccac gggttatttt ccgaactcca cccggaacca
     5701 tcagaagcac caccacctac accaagcccg gtcgggccac cattaatgtt accacttgtg
     5761 ctatgcgcgc ccgtgttatg gccgcgtcca tcgccaccgc tcataaaaat tccctctttt
     5821 aagcgttaaa acaattaatt acacgattaa tactgtacat aaaaccactg gttttatgta
     5881 cagatcaaaa agtacccgtc atcgtttttt ctgtcaagct caggtggaaa attttttaga
     5941 aatcaggagg gataacactt agaaaacgca gagcgggcca caaaaaattt tgtggcccgt
     6001 gatgtgttgg cggggtccgg ggtgcagccc cggacatcac atactcatgc cccggaagtg
     6061 cccggggcgg cgttgttgtt gcctgtgctg ttcgtcccgc ttccactcat gccaggtctg
     6121 attaaaaccg agttgacgga agagataatc cggcatctct tccggtttgt ggacagcagc
     6181 caaaagcctg tcatgatggc ctttgagccg gaactgcatc aggtgcggtt ccggtgggta
     6241 cggagtatcc tcatcatggt ttatagcttc tccgtaacct gccaggcata ccccgttctc
     6301 cgtgatggtg ctgactatca gatgactgta atccggcgac ggaagcacaa gaacggggca
     6361 aaaccctcct tcccggcgaa caaacccgac aagcatttcc tccggccctt tgcataacaa
     6421 cagattcagc gtcctgctgt ttccctctct cgaaaataca acaggcatga gttgttccgg
     6481 tacagcatca gcggtgatat ccacccggtg cgctgacggc agcggagagg ctacgggcct
     6541 gcggtcgtcg ctgaaataaa aattatccgg ccagcgatgc ggcagcgccg gaaaaccgta
     6601 agagggccac ccctggcgca tctgttccag tacatgccgg accgtcgcac cggaatacat
     6661 tctcacacgc tcaggccgcg ccatcgtctc atgagattct ggcattccct cacgtatatc
     6721 cgagaccatc acagccccgc ctggcgacag gtaaacattc accgggtcat gaacactctg
     6781 ggggtaatgc gccagctcat caccccatac cgtgaattct ccggctctgt tactcagcat
     6841 catgtagcgg gttatcctgc ccgttcccgg ctctctggca cgccccagct ccgtaataac
     6901 cccgactaat gccgtttcac ggctcagacg gtggttatca tgacgtttca gccgcgcctc
     6961 atgctcctgt tccttccggc gcgcttccgc ttcggcttcc tccctgcgcc gctccgcttc
     7021 ccgtgctgac agtttttctt tcagctctgc cattcccggc gcaatccacg tattccactg
     7081 cgtttcaaag gcctgacgac gggcgggaat gtcccgttct ttcacggccc tgtaggtgcg
     7141 gcgaagagat tcaatctgtt tcccgagttt ccagaaacgg agttgtcttt tgcggtattc
     7201 cggttcagcc catgtcatca gggggatctc tgacagaagg gacgcgacag ggcgtgcttt
     7261 tttgagtccg gtcatctcct tttcgagaat ggcctgctct ttcagcagcg cattcccctc
     7321 acgttcgatg tcgttcacct tcccgctcat cgccaggaac tccggggtca tctcccacgc
     7381 gttgccgcat tcctgcggca acgtggcaat gaattcccgc agggtatccg ctgaggcatt
     7441 cgcagcggga atggtcaggt taagggcttc cgctgcacga cgtgcggcat cgctgagttc
     7501 ccgtttccgc acgttctcct gtacctggat gactgccagc cttgtgcggg aaacctgtat
     7561 ttccaggtta aggctgttaa tcagacggtt ctgctcaccg cgttctgttt ccattccccg
     7621 tttttccatc gcacaggcag cctttccgag gtgaaggccg ggcgctttct ccagtccctg
     7681 acgctcgtat gaacgatggt ctatctcttc ctggtagccg gcgttcgcaa gcgctctgtt
     7741 cgcatggtca gcccatgatg cgcgccacgt ctcggcgagt gcccggtcgt tccagtccct
     7801 gacctttcct gcaaatcccg tttccccgac agctctcgtg gtcagcatga tgtgcgcatg
     7861 gggattggtc cggtccatgt gatggaacgc cacatcggca atcatgcccc gactgacaaa
     7921 gttttcccgg acaaaagcga gaacggtctc ccgtgcggcc tcgcgggaaa tctcacgggg
     7981 aatggcgagt tcaatctccc ttgccagctg ggagttccgg cgctgttcgg ctttctcgac
     8041 cgcattccag agaacggaac ggtcaccaca ccaggacggt gcattcgcag gggtgagaat
     8101 gaccgcatca tcaacgccgc gtttacgggt gtaatcgtgg gttaatccgg tacggtcatc
     8161 gggtatgaca gagccggaac ggtaggcagc agaagcaacg gcactgtagc cgttttttcg
     8221 cgaaatgatt ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgccg
     8281 ttttctttta gcgtcgcaga ccgcacacaa tcgaaacgaa gtggagattg tataagtgcg
     8341 cacttcgtgc acttcgtgat gtgccgtgca ttatgccagt tcagttttgc aactaccagt
     8401 gacgcgctaa aatgcgattt ttcaccgcgc acggagtcac gaaatgagca gaacactcga
     8461 acagaagatt gccgaagctg aggccagatt acagcgcctg aaggcgaaga gccgaagcct
     8521 ggacacggcg caaaaagtca ttgtgggggc agccatgctg gcaagggtac ggaggccgga
     8581 agaagcgcaa ctgcgtgcgt tcctgctcca gtttctgcga aaggaagtca caagacaggc
     8641 ggatgtgaac cgactacaac cgctgattaa cgagctggag aagctaccca gaccaccggc
     8701 gaaaccgcag aaccactaag ccccggcgcg taagtgccac atgcaccacc ggacgccttt
     8761 gcgcttcctc gctcattcgc tcgctacgct cggacatgag actgcggcga gcgctaccac
     8821 ctcaatcatg cggaaagcca caccattgcg ggattcaacg agggaaatag caacaaacca
     8881 agctgctcaa aaagacgcca aaaaggcgct gttatctgat ataggcttat ttggtctact
     8941 ctacgagacc tcgttttttc agttggtaat accaggcgcg actgatccct aatttctccc
     9001 aaggtttcag cgtgcgtgct gatgtagcta ctgtgccgcg cttagactta gaaccgccct
     9061 tgcgaccgcg tgtagcctga atttctggcg tgtgcgtatc agccacatac tgtgcgaaag
     9121 tttccggcgt gaagttcctg tgcgtgtatt tcgcaatact cttgccaata gcccgacatt
     9181 caggaggtga aagcggaacg gggagcgatg cgttgtacat ttcgacacgc tgaatcaccg
     9241 catcaagcca ttgtgagaat gccggccagc cctgacgaat agcccggtaa gcccatttac
     9301 ggcccttttc gaacagatag cagtttcgcc ccagcccgta atttttatcg acgctacggc
     9361 gcgctgaggc gctcaaatcg agataatcag ccagttcgtc gagagtgtag ggttcctcgc
     9421 gccattccac ttcctgccat tcagggtggc acggattttt gcagatcagg ccgctgtaat
     9481 tcacatccgc gcccagtttt tcacacaacg cacgctcaat agcggcagcg tatctgagcg
     9541 ccgaagccga tgcatccggc gcagttctca caggaagggc gagcgcgtag agcagatgcg
     9601 cgtgcccgtt acggggattt tttacggtga tgttcggggc cggacaattc cggtcgctcc
     9661 agtcgattga tgcccccgtc cggtccacgt caaagggaag ccagtacagt gcatgtggct
     9721 ggttctgctg gatgaaacgg gcaagaatgg caatgttttt cggcagaatg cgaacgccgt
     9781 acgcgaaatc gttcgtacag tacggtttgt gcggtaattt ttccctgaag cgttgaagta
     9841 cggcgctcat gtgatacgat ttctctcggt acacagttta actaaccgcg ctttcccatc
     9901 gggaaacgcg gaacccggcg ccctgtaatt tcgtgatcaa tcttggcggt gagagcacga
     9961 tttacggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
    10021 cttcccgaaa aaccgatcca tttaccataa ctcccgttgt acgtacccct taaaacaccc
    10081 tcaatcgctt tctgacgcaa aaaacacaaa aagacaagga aacaccaggg taaacataaa
    10141 aaatgcgtca gaatcgtttt tagcgcgttg cataactatg cacgaaagcg atacacgatc
    10201 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaca agttttctcc ccctggatgg
    10261 aaagttctgc acgagaattt tagatcccat ctcccacatg gaatttttgt gacatttcaa
    10321 aaaaaagcgc atcagaatca gcctgacacg ctttgttttt gtcaaagagg atttcgggtt
    10381 actgcgttgc taatccggtc acttcagctg ttgatgacgg agatacggtc ccgccctgaa
    10441 catcccggat atagtttgcc tgacatgcag ccagaataat gactgcaaga agcaataaaa
    10501 taatccctgt tatttttttc ataacgactc cttgttgtga aagaatgcca gtcattcatc
    10561 cttaaacaac tggcattcat atttcagccc tgtttaaatc ctgacttacc gttagcagct
    10621 cgccattctt taatctcttt aataacagct tcagggctac catcattatt accttctggg
    10681 taataaatca gatcagaacc actaggatgc tcagtaacac ttataaaatg ctcgaggtta
    10741 tcatcctgtt ttttttcatc accttcacaa ttgatgatgt cttcaataat ttttttgaat
    10801 tcagtttcag tgtaatcact aatgctgttt ttcagttcca tttttactga cctctgtgaa
    10861 tatcaatatg gcgtttcggc gtagtgatac ggaggttgtc catatcataa acaccaccat
    10921 cctgactgat tggcttgtcg tgatgaagct caaatgagcg acgcccaccg acagtatctt
    10981 tattcctagc cgtggagcta atccttggct caagcgtttt ttattacctg gattaaattg
    11041 cttacttaac tcaggatctt tcgacacttc ttcccagaat tttctccgaa aatcgtcgaa
    11101 gtttttaaat tctttatcac gcaacttatc agcaatgcga
//
