LOCUS       NZ_CP129347             6988 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain STEC1590 plasmid p_STEC1590_2, complete
            sequence.
ACCESSION   NZ_CP129347
VERSION     NZ_CP129347.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36269620
            Assembly: GCF_030438435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Prevalence and Characterization of Shiga toxin-producing Escherichia
            coli isolated from asymptomatic carriers in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, NO.155 Changbai Road, Changping District, Beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 42.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:13:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,150
            CDSs (total)                      :: 5,026
            Genes (coding)                    :: 4,825
            CDSs (with protein)               :: 4,825
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 97 of 201
            Pseudo Genes (incomplete)         :: 116 of 201
            Pseudo Genes (internal stop)      :: 51 of 201
            Pseudo Genes (multiple problems)  :: 59 of 201
            Pseudo Genes (short protein)      :: 3 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129347.1.
            Bacteria and source DNA available from China CDC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC1590"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p_STEC1590_2"
                     /geo_loc_name="China: Guangxi"
                     /collection_date="2021"
     gene            complement(join(6730..6988,1..65))
                     /locus_tag="QYF45_RS25700"
                     /old_locus_tag="QYF45_25700"
     CDS             complement(join(6730..6988,1..65))
                     /locus_tag="QYF45_RS25700"
                     /old_locus_tag="QYF45_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            133..324
                     /locus_tag="QYF45_RS25705"
                     /old_locus_tag="QYF45_25705"
     CDS             133..324
                     /locus_tag="QYF45_RS25705"
                     /old_locus_tag="QYF45_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1330..1461)
                     /locus_tag="QYF45_RS25710"
                     /old_locus_tag="QYF45_25710"
     CDS             complement(1330..1461)
                     /locus_tag="QYF45_RS25710"
                     /old_locus_tag="QYF45_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            1491..1781
                     /gene="cki"
                     /locus_tag="QYF45_RS25715"
                     /old_locus_tag="QYF45_25715"
     CDS             1491..1781
                     /gene="cki"
                     /locus_tag="QYF45_RS25715"
                     /old_locus_tag="QYF45_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(1778..3424)
                     /gene="cka"
                     /locus_tag="QYF45_RS25720"
                     /old_locus_tag="QYF45_25720"
     CDS             complement(1778..3424)
                     /gene="cka"
                     /locus_tag="QYF45_RS25720"
                     /old_locus_tag="QYF45_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            3664..3954
                     /locus_tag="QYF45_RS25725"
                     /old_locus_tag="QYF45_25725"
     CDS             3664..3954
                     /locus_tag="QYF45_RS25725"
                     /old_locus_tag="QYF45_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(3946..4224)
                     /locus_tag="QYF45_RS25730"
                     /old_locus_tag="QYF45_25730"
     CDS             complement(3946..4224)
                     /locus_tag="QYF45_RS25730"
                     /old_locus_tag="QYF45_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            4346..4768
                     /locus_tag="QYF45_RS25735"
                     /old_locus_tag="QYF45_25735"
     CDS             4346..4768
                     /locus_tag="QYF45_RS25735"
                     /old_locus_tag="QYF45_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5252..5485)
                     /locus_tag="QYF45_RS25740"
                     /old_locus_tag="QYF45_25740"
     CDS             complement(5252..5485)
                     /locus_tag="QYF45_RS25740"
                     /old_locus_tag="QYF45_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5492..6052)
                     /locus_tag="QYF45_RS25745"
                     /old_locus_tag="QYF45_25745"
     CDS             complement(5492..6052)
                     /locus_tag="QYF45_RS25745"
                     /old_locus_tag="QYF45_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6084..6521)
                     /locus_tag="QYF45_RS25750"
                     /old_locus_tag="QYF45_25750"
                     /pseudo=""
     CDS             complement(<6084..6521)
                     /locus_tag="QYF45_RS25750"
                     /old_locus_tag="QYF45_25750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(410..497)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          508..1064
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(125..327)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="99.0"
                     /evalue="3.86e-12"
     Typing          complement(5146..6740)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttttccct cacaacgtgc cagcagccgc gcatgttcct catccgtgac ccgtatcgtg
       61 agcatccttt cgcgtttcac cggtatcatt aaaaacctcc gacagactcc ccacacatgg
      121 agaaacagaa ctgtgactaa acaggaaaaa accgccctta acatggcccg cttcatcaga
      181 agccagacgc tgaccctgct ggaaaaactg aatgaactgg acgccgacga ccaggctgac
      241 atctgcgaag cgcttcacga ccacgctgac gagctttacc gcagctgcct cgcacgcttc
      301 ggggatgacg gtgaaaacct ctgacacatg cagctccctg agacggtcac agctggcctg
      361 tgagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggttt tagcgggtgt
      421 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaatcatt
      481 tggcatcaat gaggattgta tgaaaagtgc accatgccgg gtgtgaaatg ccgcacagat
      541 gcgtaaggag aaaatgctcg tccaggcgct tttccgcttc ctcgctcact gactcgctcc
      601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaagacggta atgcggttat
      661 ccacagaatc aggggataac accaaaagaa acatgtgagc aaaaaacaag aacccggaaa
      721 aggccacgca gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaacc
      781 gacgctcaag tcagaggtgg cgaaacccga caggacttaa agataccagg cgtttccccc
      841 tggtggctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      901 ctttctccct ttgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc
      961 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1021 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttaacgcc
     1081 actggcagca gccattggta actggatagt ggatttagat acgcagaact cttgaagttg
     1141 aagccttata gcggctacac tggaaggaca gcatttggta tctgtgctcc actaaagcca
     1201 gttacccggt taagcagtcc ccaactgact taaccttcga ctaaaccgcc tccccaggcg
     1261 gttttttcgt ttacaggcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1321 tgatcttttc tactgaaccg cgatccccgt cagttcagaa gacgaagatg gtgcaacggt
     1381 tcctccttgt acatcacgaa tatagtttgc ttgacatcct gacaagaata atattgtaat
     1441 tagaaaaatt attattttca tatttctttc caacaaaagt aaaaatactt atgcatttaa
     1501 aatactacct acataattta cctgaatcac ttataccatg gattcttatt ttaatattta
     1561 acgacaatga taacactcct ttgttattta tatttatatc atcaatacat gtattgctat
     1621 atccatactc taaattaacc atatctagat atatcaaaga aaatacaaag ttaaaaaaag
     1681 aaccctggta cttatgcaag ttatctgcat tgttttattt attaatggca atcccagtag
     1741 gattgccaag tttcatatat tacactctaa agagaaatta aatccctaac aactcattaa
     1801 gtttgctcaa ctcatcatcc cctatgtaag aagaaacaat acctgtgaca attgcaatcc
     1861 cccagaaacc aagaggcaca ccaacaataa aactaaacat taatgccaca atttttgcca
     1921 cacctacatc gaccgcactt ttctccagcg tgacaaacaa aggtcgccag ttaccagttt
     1981 ctattgcatt tttaaaatca gaaccaacat catatagaaa agatactcga ctggtgattt
     2041 taagcgactt tgatatcttc gttaaattct tagataactc atcataatta acagactcta
     2101 acgcattaaa aatagcatcc cgatcaacct tactgaattt cttatccagt acattcttat
     2161 acttttcaaa tgctgccaga gcttcatcaa caccttgaat tttctttcct ttagactttt
     2221 ccgctaaatc ctgagcaatt tttgcgtatt tttctccata ttgttcggtt atatattgat
     2281 aaaatccaac catagtatca acagcatctt ttatctggct cttttcaaga gcatcctgag
     2341 cttcttttaa tttctgctct gcctctttaa cctcagcggc cttaccatcc ctgaaactaa
     2401 cagcattctt aagttcattt tcaactgcgg agtactcagc ctgcttagct tcaagctgac
     2461 gctgtagttt tttctgaacg tcacgccatc ccggaaatcc ggtgaccttc acgtcaacac
     2521 tcttctgagc gttttcaagc tctcctgcaa ctctggaaac agtagcttgc ttactctgta
     2581 catcactttc agccttcgcc agttcagctt tcgcctcagc taaacgcttc tctgcttccg
     2641 ccaccgcctg tttctcttca ttaagagttt catgttcagc gacctctgca ttttcacgag
     2701 ccttttcttc agcttccttc tgtttattca catcaccaat tttactttgc aatgtatttt
     2761 tataagaatt aagtttgcta atatctgcat caagctgatt tgattttttt tgcaactcat
     2821 caacatccct ctcaagatca gtgataccat gataagaatg atgcttgaat acttttttca
     2881 tctcctcgat tttcttctgt ttttcactaa tctgtgtagc aattttattc ttctgctttt
     2941 gtttttcatt aattacatta cccaccacct tcgaactctt atccatatca ctgacctgag
     3001 cattcgttgg tgcagtatta actgaagcag tgttattgtt gttatttcct gagctttctg
     3061 caaaaagtga tggcatatca ctaattaaag aattaagaac tctggagacc cctccaaatg
     3121 gattatcaac cagagttgaa ttctcttctg tcataacaat accattcatc accggaaggc
     3181 catcattatt aatgacaaca tcaccccacg gagtcagata tgactcaccg gttttcatca
     3241 cagttgatgt agaaccagat gaatttgaat ttccctgacc accattatta ccatgcccag
     3301 agccaccacc ccagtgaacc ccactattac tattattatt tccaccttgc tgattcagat
     3361 tcgcccctgt tccccccata gactcaccag cagttggtcc atatccactt agttctttag
     3421 ccataaattc ctctttgata attaaaacaa taaattaaaa acaatatact gtatatataa
     3481 ccactggttt tatatacagt aaaacctact actcagcatt gtccatgtca agagcatgga
     3541 ttttcatttt tgcaataagg atcacactat ggggaggcag gcattgagaa cgtcgaaaca
     3601 gaacaccgga gcaaatcagg atgagatata aaactgttgg atcatgaaaa aacggagaac
     3661 gatgtgagca aatcaccccg ccataaactg aacaaaacag acaaacgact tctcgacacc
     3721 cttgttgctg ccggatatga gcatgacaaa gcccgtgacc tcatccagaa acaggtttac
     3781 acgctgacac tggctgatca gcgtcatgtg gtcagtgaaa tcagtaatgg tgtgaatccc
     3841 acccaggctt actcggcggt ataccaggca agacgcattc gcctcgcccg taaatatctg
     3901 aacggtaaaa aggttatgga agaaaccggg gaaaatacgc ccccatcagc gtaaggattt
     3961 cttttgccgc tccagagact ccagtttttt acgcaaatcc tctctttttt gggcatctct
     4021 ggtgccagcc agctctgctc tcaactcatc gatctgaagt tgtatcttca gtctgttact
     4081 gaacattttc tgtctggcat taacatccgc aacaatgccg ttttttgtct tctcggcctt
     4141 ttgttgaaaa acattgctgt ccgcatgact ggcaaccgaa gcagaaagaa cactaaaaag
     4201 caggactggc acacattttt tcacgggatt attcctgact cattgaccat caaatcacat
     4261 tgggagtaaa ccgacgtatg ataagagata ctcttcggag atataactcc ctgagtatca
     4321 agattaaaaa cgcaaggaga tgtttatgag atctgccgct gccaggctgc ttctgatacc
     4381 tctgataaca gcaacaatag ctcttacagg atgcacacca aagaccagcc tggaacgaca
     4441 tacccggcat tatgtttatg cttcagatga tggatttgat cctaacttct acacccagaa
     4501 agcagacacc atacgtatga tgctcccgtt ctttcagcag ttccgggata tggggatgaa
     4561 agacaaagca gccggagtat cagcagaaac ggcacagcaa cgtgtaaaag aattccactc
     4621 agaaaaattt tttcactcac tccggagcac aacagccttt gctggcagaa aatacacaaa
     4681 cagcgatatg ccttcgccga aaaaaatgaa actaatggca gacaccattt ctgcggttta
     4741 tctcgatgga tacgagggca gacagtaagg gatttaccat aatcccttaa ttgtacgcac
     4801 cgctgaaatg cgttcagcgc gatcacggct gctgacaggt aaaaatggca acaaaccacc
     4861 cgaaaagctg ccgcgatcgc acctgataaa ttttaaccgt atgcatagct attcagccat
     4921 gtgaataacg ctagttttgc ctgcgtaaac ctcatgacac tgtttttttt ccatcttttc
     4981 agttgatgac atacgcagac atcgcgggat gaggctgagg aatgagcgcg atctggcaaa
     5041 gaggcaaaac acagcaacaa aaacgacacg ccagaatcgc gcccggatgc gtttttaacg
     5101 cgttccggta ccatctggca acctcccgga acaactcacc gtcacatccc tattgacggg
     5161 ccacgccata cccgtgcttc ccgttcctgc tcttcatgcc aggaccgcgc acgctcccgt
     5221 tccaggcgtg cctgcctttc ctgttcatcc cttatctgct gttcgtgata aataaccgac
     5281 tcaagtggtc cacctgcccg gctaatctct gcacctgctg actcaagtcg tcgcactgtt
     5341 ccctcagttg cccgttctcc tgtcgtgtca gctcgaacat atgctgcaaa tccgtgaagg
     5401 cgctctccca gtctttcagc cgctgcgtat agtcctgctg caattgctct aaggcgttca
     5461 gtaagtgcat ttccagctct gtcatactca cttactccct gaccagtctt actgcgttct
     5521 tcttctccac cgtccagttg ttttccccct tcaccccgga cggcaacacc agaaatttcc
     5581 cgttcctgcc ctcgtgatac gtcacacccc atgttttttc ccggagtttc gccagcgtct
     5641 cttcctggtc cctgatagcc aggatgttcg ccgcaatccg gctttcctgc cactgaatca
     5701 gcccccatga cgccagaaaa atcccgcccg tgacgcaaag cgccgtcagc gacgggtaca
     5761 gtatccgccc tttgaccagc ttccagagca gctcttcctg ccgccgggcc agttcgttct
     5821 ctgtggcgct gaactgcgcg ttcacggcac tgttcagcgt ctccagttgt tctttcaccg
     5881 ctgctgtgtg tgcgctgata gcgtctctga ttttctgccc gtttaagttc agttccctgt
     5941 ctacagacgc ttcgagcttc ctgaactcgc tgttcagcat gttctctgta gagacggcac
     6001 gctctttcag tttcttctcg aagtctgtcc ccatttgtaa aagattgctc atacagcgcc
     6061 cctttcagcc tgagattacg cccaccctcc gggtcggcga tactgatact gctcctggtt
     6121 gtcctcacaa cctcaaaacc tgccgctgta agcgcctcag tgacatcctg acgcgttttt
     6181 agcgctccgg catggtaaag agcctccagt cccctcgtaa tcgcttctgc ggcctcctgt
     6241 ttcgctttcg gcagattatt cggggtgaca agtgtccgcc tgttctccgg tgcgttcggg
     6301 tcgtgcagcc cgtaatggtg attcaccagt gtctgccagg cattgattcg cggacggtct
     6361 gctcggtcgt aatagggctg gagccgtttt ccgctcgcca gctccatatt cgggatgaca
     6421 aaattcagct caagacgccc cttgtcctgg tgctccaccc acaggatgct gtactgattt
     6481 ttttcaagac cgggcatcag tacccgctca aagctctcca tcaccctttc acgctctccc
     6541 ggtggcaggg tctgctctgc aaaagacaga acccccgagg tgtatttttt cgcaaacggc
     6601 gtggcatcga tgagttcccg cacctcttcg ggagcacccc gcagaactct cgccccttcc
     6661 cggttacgct cccggcccag caggtaatca accggaccac tgccaccgcc ttttcccctg
     6721 gcatgaaact taactatcat cccgttctcc ctgtttacgg acctcatccc tcagctcact
     6781 cagttcacgt ccgatggcca tcagtgcagc caccacatga acccggtcat gccccgacca
     6841 ctgtccgctg tttatcttcc gggctatctg attcaggtta ttgccgaccg aagcgaactg
     6901 gcgcaacagc ggcggtgcca gtgtcggaag acctgacgtt ttcgatggcg gtgcccccag
     6961 gcagacctta cgcatccatg acgcaagt
//
