LOCUS       NZ_CP129290             3146 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain GZM7 plasmid pGZM7k, complete sequence.
ACCESSION   NZ_CP129290
VERSION     NZ_CP129290.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36084424
            Assembly: GCF_030439225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Carrying blaCTX-M avian Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2023) College of Veterinary Medicine, Henan
            Agricultural University, LongZiHu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2023
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,249
            Genes (coding)                    :: 4,960
            CDSs (with protein)               :: 4,960
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 1 of 289
            Pseudo Genes (frameshifted)       :: 109 of 289
            Pseudo Genes (incomplete)         :: 188 of 289
            Pseudo Genes (internal stop)      :: 48 of 289
            Pseudo Genes (multiple problems)  :: 54 of 289
            Pseudo Genes (short protein)      :: 4 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129290.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GZM7"
                     /isolation_source="liver"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pGZM7k"
                     /geo_loc_name="China:Henan"
                     /collection_date="2013-02"
     gene            1..441
                     /locus_tag="QYN11_RS26845"
                     /old_locus_tag="QYN11_26840"
     CDS             1..441
                     /locus_tag="QYN11_RS26845"
                     /old_locus_tag="QYN11_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     gene            complement(615..1154)
                     /locus_tag="QYN11_RS26850"
                     /old_locus_tag="QYN11_26845"
     CDS             complement(615..1154)
                     /locus_tag="QYN11_RS26850"
                     /old_locus_tag="QYN11_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="WP_229997960.1"
                     /translation="MWFPVLFRFLAHSLTTFGRWGCGERCHLSHIACDSVVVLSGMSRQ
                     IALQTRSEALRRTKRVLNARQGMEGAKNAYRGSQEVRRADTPNQGLSGRQEPRQPPAKP
                     NCTEQAVTQLVGGRRPLILQQWGSGVLPRRFPVALKALPKPWQTMAKIRLSAFLALAAN
                     TNHPMVGLCQKRAEVF"
                     /name="rep protein"
     gene            1659..2135
                     /locus_tag="QYN11_RS26855"
                     /old_locus_tag="QYN11_26850"
     CDS             1659..2135
                     /locus_tag="QYN11_RS26855"
                     /old_locus_tag="QYN11_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2580..2705)
                     /locus_tag="QYN11_RS26860"
                     /old_locus_tag="QYN11_26855"
     CDS             complement(2580..2705)
                     /locus_tag="QYN11_RS26860"
                     /old_locus_tag="QYN11_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001431667.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="hypothetical protein"
     Typing          complement(1472..1580)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          2727..2825
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(825..1075)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.254"
                     /coverage="100.0"
                     /evalue="1.43e-100"
     Typing          complement(2367..2627)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccgaattta aagagccagc ggcaggcgga caatcagggg ctacgtgtta
      481 acgttttgac catgattgtc tatcctgcat tgctcttttg ccgcctcaaa atcccttgca
      541 ttttttgcac ccccgttctc cagaactacg caattcgaac aggtcctgtg actagacgtt
      601 gatcgggcaa tacgctaaaa cacttccgcg cgtttctggc ataaaccaac catgggatgg
      661 tttgtgttag cagcaagcgc cagaaacgcg ctcaatctaa tcttcgccat cgtttgccac
      721 ggtttcggca gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccat
      781 tgctgtaata tcaacggtct acgaccacct accagctgag tgactgcctg ttccgtgcag
      841 ttgggctttg cagggggctg gcgcggttcc tggcgacctg aaagcccctg gttaggggta
      901 tcggcgcgcc tgacctcctg agagcctctg taagcgtttt tcgcgccttc catgccctga
      961 cgggcattta aaacccgttt cgtgcgtctg agagcttctg agcgcgtttg aagggctatc
     1021 tgacgactca tccccgaaag aacaacaacg gaatcacagg caatatgcga gaggtgacac
     1081 cgctcgccgc agccccagcg accgaacgta gtgagcgagt gggcgaggaa gcggaagaga
     1141 accggaaacc acattgagca cgtacgcact gatgcggggt gtcggggtga agccctgacc
     1201 agatggcaaa cggaatatcg tcgcgggtga cggtattaca tttgcacatc ctgtcccgat
     1261 ttctgcggag tttaacggtg aacggacggc aaaaaacgtg caggagctgt tgaacgtgaa
     1321 aaccacgaaa tctggcaaaa atcacagctg acgaggcttg cagaggagta agccagtata
     1381 cactccgcta tcgctacgtg actgggtcag ggcttcgccc cgacaccccc aaagggcgtt
     1441 gcgttgcacg caacaccctt gcctagaata gatcttttac gccgatttgt aagtggttgt
     1501 ttttttagtg tgttttttat ttacctgtcg catccaggat gacacaacgt tgtcgcatcc
     1561 tggatgcgac aacacgcaac aaggtgttgc acagatgtgt catgatggct tagatttgaa
     1621 ccaacgttag tgcatgggat tttcagaggg aaaaaatcat gtttattgat tcagaaaaac
     1681 gactgaaaca actttcagat gaggcaaaga aaaacaccga ggatctcgaa gaagcaaaga
     1741 aaaattcaag gtttacacag gtatccccaa aaggttggga acgtgttcga gagctgctga
     1801 aggatagcca aggcatatca gcactgaagc tgtactcatt tttagcggag catatcgatc
     1861 ctacgtgtgg cgctgtcgtt gcggatcagc aattcctagc tgaaaaactt ggagttagca
     1921 gaagcacaat tattcggtgg ctcaattact tagaatcaaa aaatgcatta gttagaatcc
     1981 ccgttgctgg taaggtttgt gcgtatgccc tcgatccaca tgaagtctgg aagggataca
     2041 acactacgaa aaaccatgca gcgtttgtca ctaaaacact ggtcaacaaa gacggtgata
     2101 ttcagcgccg aatcatggcc atgttttcaa attgagctag cggcaggcgg acaatcaggg
     2161 gctacgtgtt aacgttctga ccatgattgt ctatcctgca ttgctctttt gccgcctcaa
     2221 aatcctttgc gtgtttttgc tccccgttct ccagaaaaaa ccgagccgcc acggttccgg
     2281 cagcgccttg agcgctaccg gaaaccgtct ggggagaacc ccagaccccc atcgctgcaa
     2341 caccgacggt ctacgaccac ctaccaaccg catggctgcc tgttccgtgc aaatgggctt
     2401 tgcagggggc tggcgcggtt cctggcgacc tgaaagcccc tggttagggg tatcggcgcg
     2461 cttgacctcc tgagagcctc tgtaagcgtt tttcgcgcct tccatgccct ggcgggcatc
     2521 taaaacccgt ttcgtgcgtc tgagagcttt tgagcgcgtt tgaggggcta tctggcgact
     2581 catccccaaa agaacaacac cgggatcacc gacaacatgc gagaggtgat cccgctcgcc
     2641 gcaagtcgat gaccgagcgt agcgagcgaa tcgacgagga agcggaagag aaccggaagc
     2701 cacattgagc acttacgcac tgatgcgggg tgtcggggtg aagccctgac caagtggtaa
     2761 tcgtatcggc gtgcatgcgc ggttatacga ttacacatcc tgtcccgatt tctgaggcgt
     2821 tttaacggtg aacggacggc aaaaacacga tggagctgtt gaacgtgaaa accacgaaat
     2881 ctggcaaaaa tcacgtctgg cgaggcttgc ataggagtaa gccagtatac actccgctat
     2941 cgctacgtga ctgggtcatg gctgcgcccc gacaccccaa agagcagcta ggataaatat
     3001 ctaatggaat caatgcgtta gatgcctaat ataaagatac agaaaggaga ataaaaacat
     3061 taataaaatc taagggttac agttgacata gttatcaata atgataacac tgttatcatt
     3121 attgataact caaatagggt tcaaag
//
