LOCUS       NZ_CP129289             3373 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain GZM7 plasmid pGZM7j, complete sequence.
ACCESSION   NZ_CP129289
VERSION     NZ_CP129289.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36084424
            Assembly: GCF_030439225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Carrying blaCTX-M avian Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2023) College of Veterinary Medicine, Henan
            Agricultural University, LongZiHu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2023
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,249
            Genes (coding)                    :: 4,960
            CDSs (with protein)               :: 4,960
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 1 of 289
            Pseudo Genes (frameshifted)       :: 109 of 289
            Pseudo Genes (incomplete)         :: 188 of 289
            Pseudo Genes (internal stop)      :: 48 of 289
            Pseudo Genes (multiple problems)  :: 54 of 289
            Pseudo Genes (short protein)      :: 4 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129289.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GZM7"
                     /isolation_source="liver"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pGZM7j"
                     /geo_loc_name="China:Henan"
                     /collection_date="2013-02"
     gene            join(3061..3373,1..11)
                     /locus_tag="QYN11_RS26820"
                     /old_locus_tag="QYN11_26815"
     CDS             join(3061..3373,1..11)
                     /locus_tag="QYN11_RS26820"
                     /old_locus_tag="QYN11_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>660
                     /locus_tag="QYN11_RS26825"
                     /old_locus_tag="QYN11_26820"
                     /pseudo=""
     CDS             220..>660
                     /locus_tag="QYN11_RS26825"
                     /old_locus_tag="QYN11_26820"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1249
                     /locus_tag="QYN11_RS26830"
                     /old_locus_tag="QYN11_26825"
     CDS             689..1249
                     /locus_tag="QYN11_RS26830"
                     /old_locus_tag="QYN11_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1256..1489
                     /locus_tag="QYN11_RS26835"
                     /old_locus_tag="QYN11_26830"
     CDS             1256..1489
                     /locus_tag="QYN11_RS26835"
                     /old_locus_tag="QYN11_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2806..2997)
                     /locus_tag="QYN11_RS26840"
                     /old_locus_tag="QYN11_26835"
     CDS             complement(2806..2997)
                     /locus_tag="QYN11_RS26840"
                     /old_locus_tag="QYN11_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2642..2725
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          2..1572
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.817"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3029..3372
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.348"
                     /coverage="97.0"
                     /evalue="2.15e-56"
ORIGIN
        1 atgatagtta aatttcatgc cagggggaaa ggcggtggaa gtggcccggt cgattacctg
       61 ctggggcggg agcgtaaccg cgaaggcgca acggtgctcc ggggtaaccc ggaagaagtg
      121 cgggaactca ttgattccac gccattctcg aagaaataca cttcaggcgt tctgtcgttc
      181 gcggagaagg agttaccgcc gggtgaacgc gaaagagtga tgacgagctt tgagcgtgtt
      241 cttatgcccg gtctcgaaaa agaccagtac agcattctct gggtggaaca ccaggataag
      301 gggcggctcg aactgaattt tgtcatcccg aacatggagc tacagagcgg taagcgcctc
      361 cagccgtact acgaccgcgc ggacaggccg aggataaatg cctggcagac gctggttaat
      421 catcattacg ggctgcgtga cccgaacgca ccggagaaca gacggacgct tgtcacgcca
      481 aataatctgc caaaagcgaa acaggaggcc gcagaagcga ttacacgggg tcttgaggct
      541 ctttaccatg ccggagcaat aaaaacgcgt caggatgtca cagaggcgct tacagcggca
      601 ggttttgagg tggtgaggac aaccaaaagc agcatcagta ttgccgaccc ggaggggggg
      661 cggaatctca ggctaaaggg ggcgctgtat gagcaatctt ttacaaatgg gaacagactt
      721 cgagaagaaa ctgaaagagc gagccgcttc taccgagaac atgctgaaca gcgagttcag
      781 gaagctcgaa gagtctgtag acaaggcatt gagcttaaac aggcagaaaa tcagagacgc
      841 tatcagcgaa catacaacat ccgtgaaaaa gcaactggat acgctgagca ccaccgtgag
      901 catgcagttc agcaccacag aggccgaact gtcccggcag cagaaaaaac tgctctggcg
      961 ggtgatcaaa gggcggatac tgtttccggc gctgaccgcg ctcagcgtca cgggcggcat
     1021 tttcctgggg tgctgggggc tgatggagtg gcaggaaagc aagattgcga aaaatatcct
     1081 gactatcaga gagcaggaaa acacgctggc gaaactcgag gcaaaaacct ggggggtgac
     1141 gttcgtcaat ggcgagaacg ggaaatttct ggtgttgccg gacggggtga aaggggaaaa
     1201 cacctggacg gtgggcgaca agaacgccgt gaggctggtc agggagtaaa cgagtatgac
     1261 ggaactggaa gggcatttgc tgaacgcctt agagcatttg caacaggact atatgcggcg
     1321 gctgaacgag tgggagagcg ccttcgcgga attgcagaag atgcacgagg ttacgcaacg
     1381 gaacaacgcg atactgaacg agcgcgtcgt gatattgagt cagcaggtgc agagattagc
     1441 cgggcaagtg gatcgcttga gtcggttatt tatcgcgaac aacagctaag ggatgaacag
     1501 gagcgtgccc ggtacagaca tgaggtacag gaacgcgcgg cacgggttcg gagtgggcca
     1561 tcaatggaga tgtgaccggg gaagtgacga aatcttgcca ttttgaccag ttcgacggct
     1621 ggggcgagcg aaacaaatcg tgtgccgtat ttgtgcatag ctatgcaaca tcctgaaaac
     1681 gcatttcacg cgtttttggg gattcagcca ggggaaaaca tggttttttt actttttgag
     1741 cgtaaagacg tgaaaaatct cgttattgca cttgcgatct ttcgtcggcg ggcgtagaac
     1801 gaaaacagct cgccaggaag gcgagctgtt tcgggttcat tgaggtctct gacctcctca
     1861 atgttcctgt ggaaactgtt gagatccaca acagaaacca gttcattcag tttgcatgag
     1921 ccttctgtat tcttcaagaa tttttcaaca gcacagtctc ccaggaaccc cgaaaaaaac
     1981 ctgtttttgc agatacttgc gtatttgcga gaatcgtttg cggttggggg agctgtgacg
     2041 acggcgaccg cacgcgtgag agttccgcta ccggcgcaga gagtttcatc gacagcggac
     2101 acctgatgag ttgccacaaa aaccgtatgt agcgcggcta gattggttga gttagacctt
     2161 ggcggcagtt cccggtatgt ggtggcgtga cgggtgagat ggtgcggcga agacgcatac
     2221 gtcacgcagg cgcgggttct tccactgacg cgccccgtga tcgatggcct tggctccgtc
     2281 cggcatatcg agagcggcaa actacccgtc ccttcactgg gaggggtgag ttgttgccgt
     2341 tccctccaac ctttcaccat ccggcatatc gcacgatccc atgtgcaccg caactcctga
     2401 taactccagt gcaaaaaaaa aaaacggatc tgcatcacaa cgagagtgat cccaccgctc
     2461 gccgcagccc cagcgaccga acgtagtgag cgagtgggcg aggaagcgga agagtcacag
     2521 atgcaagcat tgtgcactta cgcgcctgtg ccagtgtccg cgtgtgtgct gtttatcggg
     2581 tgcgctgttt ttacatctgt gcggcttttt actcctgaca tggtgcactt cccatccatg
     2641 tctgcaccga tgcaaaatga gataagccag tatacacatc gctatcgctc tgtgaactgg
     2701 gtcagggctg cgccccgaca cccgcactga cgcgccctgg cgggcttgtc tgctcccggc
     2761 atccgctcac aggcaagctg tgaacgtctc cgggagctgc atgtttcagt tgctttcacc
     2821 gtcatcgccg aagcgtgcga ggcaactgcg gtagagttcg tcggcgtggt cgtgaagtga
     2881 ttcgcagatg tctgcctgtt cgtccgcgtc cagatcgttg agtttttcca gcagggttag
     2941 cgtctggctt ctgataaagc gagccatgtt aagggctgtt ttttcctgtt tggtcatggt
     3001 cttaaggctc cttgtatggg ggatttgtta gggggaatga taccgatgaa acgcgagaag
     3061 atgctcacga tacgggttac cgatgaggag cacgcccggt tactggaacg ctgcgagggt
     3121 aaacgactgg cggaatggat gcggcgggtc tgtctggggg agccggttgc acgcacagga
     3181 aaactgccga cactatcgcc gccattgctg cggcatctgg ctgccatcgg gaacaacctg
     3241 aaccagacag cccggaaggt gaacagcggc cagtggtctt cgattgaccg ggttcatgtg
     3301 gtggccgcac tgatggccat cgagggtgaa ttgcgccagt tgcggcaggc tgtcagggaa
     3361 cagggggtac ggg
//
