LOCUS       NZ_CP129288             3598 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain GZM7 plasmid pGZM7i, complete sequence.
ACCESSION   NZ_CP129288
VERSION     NZ_CP129288.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36084424
            Assembly: GCF_030439225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Carrying blaCTX-M avian Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2023) College of Veterinary Medicine, Henan
            Agricultural University, LongZiHu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2023
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,249
            Genes (coding)                    :: 4,960
            CDSs (with protein)               :: 4,960
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 1 of 289
            Pseudo Genes (frameshifted)       :: 109 of 289
            Pseudo Genes (incomplete)         :: 188 of 289
            Pseudo Genes (internal stop)      :: 48 of 289
            Pseudo Genes (multiple problems)  :: 54 of 289
            Pseudo Genes (short protein)      :: 4 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129288.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GZM7"
                     /isolation_source="liver"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pGZM7i"
                     /geo_loc_name="China:Henan"
                     /collection_date="2013-02"
     gene            1..1518
                     /locus_tag="QYN11_RS26800"
                     /old_locus_tag="QYN11_26795"
     CDS             1..1518
                     /locus_tag="QYN11_RS26800"
                     /old_locus_tag="QYN11_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_000739598.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRST
                     DIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGVGDCVLINNNIANDISIFNM
                     ANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYVSPLA
                     EYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLFARRFE
                     KNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESI
                     SGENGNLRGYIFELFVSSQICNFYGYGNVRINKEYKINGKHAEADVVLETNDDIYIVEC
                     KNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDKFKC
                     NNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     gene            complement(1723..1881)
                     /locus_tag="QYN11_RS26805"
                     /old_locus_tag="QYN11_26800"
     CDS             complement(1723..1881)
                     /locus_tag="QYN11_RS26805"
                     /old_locus_tag="QYN11_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532985.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVLQSS"
                     /name="replication initiation protein"
     gene            complement(2377..2571)
                     /locus_tag="QYN11_RS26810"
                     /old_locus_tag="QYN11_26805"
     CDS             complement(2377..2571)
                     /locus_tag="QYN11_RS26810"
                     /old_locus_tag="QYN11_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041642.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2843..3166
                     /gene="mobA"
                     /locus_tag="QYN11_RS26815"
                     /old_locus_tag="QYN11_26810"
     CDS             2843..3166
                     /gene="mobA"
                     /locus_tag="QYN11_RS26815"
                     /old_locus_tag="QYN11_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          2609..2834
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="99.0"
                     /evalue="4.9e-75"
     Typing          complement(1814..2332)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.702"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2844..3163
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="8.39e-60"
ORIGIN
        1 atgaaaaaaa acattgttag acaagtgctt gagagttatg gtccgtgcat atcttctgaa
       61 cttgctgaga ggattaagcg tcagaatcct tctatgtcat ctgatgcaat acgtaaaatg
      121 atctctcgaa gcactgatat tggtaagttg ccatttttaa gatttagtca taacaggaga
      181 ttcatttatc taaaagatga ctttggtagc ttcaaatttt ggcgggcgct caaaaaatgt
      241 atgcgtgagg caaattcggc gtattctcac gctatacttt ccgtaattaa taatggcgga
      301 tacttgaagg taaaggattt cgggattgtg tctggctctc cgataaagca ggccaagcat
      361 ttaagttatg aatctgttct aaaaaatctg ctttcagcta aaatacttag atctgtgtat
      421 attgatggtg ttggtgattg tgttttgata aataataata tagctaatga tatcagcatc
      481 tttaatatgg caaattgcga gtcttttttt gataagccaa tatttgaact gattaaggca
      541 tggttgcgaa atttaggtat tgtggctttt aatcaaataa aaacaaaata tgatggagag
      601 aacaatcctg tggttggttc tttcgagtgg gatataacag cgccaagtta tgtgtcccct
      661 cttgctgaat attccagcga tggactcatc ccaggttttg ttgtctgtga ttttgctcta
      721 gggtttaatc gaaatgaaat taccaccgat gctgctgata ccttcataag aaaagttcag
      781 atgaccaagt cctctaggac aaaccagagg attatgtttg ttctttttgc tagacgtttt
      841 gagaagaatg ccttcaacaa gctaaggagt gaaggggttc tggctataac tatagctaat
      901 gcgtttggta ataaggtcga tgagagcttg gccagactat cgaaggtgat tcagggaact
      961 ttatctattg aaagacatcc tgatgagtta cttcaaatgg taaaagatct ggagtctatc
     1021 tctggagaga atggaaattt aagggggtat atttttgagc tttttgtttc ctctcaaatt
     1081 tgtaatttct atggttatgg aaatgttcgt attaataaag aatataaaat aaacggtaaa
     1141 catgccgaag ctgatgttgt attggaaact aacgatgata tttatatcgt tgagtgcaaa
     1201 aatgttaaga ctttaccttc gatggaggtt agtctttgga tgaaaacaag agtgccaatt
     1261 ataaattcct actacaaatc aaataaccct gataacaaaa atatacatca tcgtttgtgg
     1321 gttacgggga atatatatcc taaagatata aaccgattgg acaagtttaa atgtaataat
     1381 aagaagattg atgttgatta tttatttggt cagtctttgg atgatttttt ctaccaaaaa
     1441 aaacaagtgt ttaatttata taggaaagtc atttcgccag attataaaaa agggcaaata
     1501 cccgattttg acttatagaa tgacgttttc tagtctctgc atttcttgac gatgacggct
     1561 tgaaaagcgt atcttcctcc atgaaacgaa aaaaccaccc ggcaaggtgg tttttcgaag
     1621 gttagttaat cctggaagat tacctaacct aggtaactgg cttcagcaga gcgcagatac
     1681 caaatcctgt cctttaagtg tagccgtagt tggggcatca cttcaagaac tctgtagtac
     1741 ggaccgcatt atcatcgctc tgctattcca gttaccagtg gctgctgcca gtggcgataa
     1801 gtcgtgtctt ttcgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1861 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgggccgag
     1921 ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1981 gtatccggta aacggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2041 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tggcttgagc gtcgattttt
     2101 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc accggcgcgg ccttttccgg
     2161 gttcttgttt tttgctcaca tgtttctttt ggctttatcc cctgattctg tggataaccg
     2221 cattaccgtc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgtagcga
     2281 gttagtgagc gaggaagcgg aagagcgcct tatgtggcat tttctcctta cgtaccggta
     2341 taccgttctg catcctgtct ggagcaggat acactgttat accccggtgc cactttccgc
     2401 ttcaaagcgt gtctgtatgc tgttctggag ttcttccgcg agttcgtgca gcttctcaca
     2461 catggcggcc tgttcgtcgg catcgagtgc gtccagcttt tcgagcagcg tcaggctctg
     2521 gctttttatg aatcccgcca tgttgagtac ggtttgctgc tgcttgttca tctttctgtt
     2581 ttctccgttc tgtctgtcct ctgcgtcgtg tgattatatc gcgtaccact tttcgaccgt
     2641 cttaccgccg gtattctgcc gacggacatt tcagtcagac aacactgtca ctgccaaaaa
     2701 acagcagtgc tttgttggta attcgaactt gcagacagga caggatgtgc aattgttata
     2761 ccgcgcatgc atgcacgcta ttacaattac cctggtcagg gcttcgcccc gacaccccgt
     2821 gtcagatacg gagccatgtt ttatgacaaa acgaagtgga agtaatacgc gcaggcgggc
     2881 tatcagtcgc cctgttcgtc tgacggcaga agaagaccag gaaatcagaa aaagggctgc
     2941 tgaatgcggc aagaccgttt ctggtttttt acgggcggca gctctcggta agaaagttaa
     3001 ctcactgact gatgaccggg tgctgaaaga agtcatgcga ctgggggcgt tgcagaaaaa
     3061 actctttatc gacggcaagc gtgtcgggga cagggagtat gcggaggtgc tgatcgctat
     3121 tacggagtat caccgtgccc tgttatccag gcttatggca gattagcttc ccggggagaa
     3181 aactgtcgaa aacagacggt atgaacaccg taagctccca aagtgatcac cattcgcttt
     3241 catgcataac tatgcagcga gctgaaaacg atcctgacgc atccttccta tttttccggg
     3301 gtaaaacatc tctttttgcg gtgtctgcgt cagaatcgcg ttcagcgcgt ttcagtggtg
     3361 cgtacaatta agggattatg gtaaataggg cggttgatat atgatgatga aatgtcacaa
     3421 aagtaacatc ttgattttta aggataaatg tcgcaggtgt gttttttgtg atatgttgct
     3481 tttgttaaat atttgatttt tatgattttt ttctttgtgt ttttttttgg ggatctatag
     3541 atattacacg tagtgtaatt ttataaatag cgtagtgtga caaaaataag atgaattt
//
