LOCUS       NZ_CP129287             3895 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain GZM7 plasmid pGZM7h, complete sequence.
ACCESSION   NZ_CP129287
VERSION     NZ_CP129287.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36084424
            Assembly: GCF_030439225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Carrying blaCTX-M avian Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2023) College of Veterinary Medicine, Henan
            Agricultural University, LongZiHu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2023
            Assembly Method                   :: Unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 384.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,249
            Genes (coding)                    :: 4,960
            CDSs (with protein)               :: 4,960
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 1 of 289
            Pseudo Genes (frameshifted)       :: 109 of 289
            Pseudo Genes (incomplete)         :: 188 of 289
            Pseudo Genes (internal stop)      :: 48 of 289
            Pseudo Genes (multiple problems)  :: 54 of 289
            Pseudo Genes (short protein)      :: 4 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129287.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GZM7"
                     /isolation_source="liver"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pGZM7h"
                     /geo_loc_name="China:Henan"
                     /collection_date="2013-02"
     gene            1..1542
                     /locus_tag="QYN11_RS26785"
                     /old_locus_tag="QYN11_26780"
     CDS             1..1542
                     /locus_tag="QYN11_RS26785"
                     /old_locus_tag="QYN11_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_049088048.1"
                     /translation="MASYHCTVKTGAKGAALKHADYISRSGEYKSYKSREDLEFSSSGN
                     MPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKKASI
                     PQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGIKRTPEVHLGPVHAA
                     NLNEGQIAAIQEHRAARQQERAARNPAPERQQELEQKQKIRTVERSPELLAQYRKVMKT
                     VIQGEERLARLGDANPDALKEHKLLQNAKAKKESLSEWSRRIYEGARYLDKLGRNVVSA
                     QRELRELQEQRNALNGIRGLFRGADKREIDARILEQESVLETAEREENEFRYKLQQAES
                     EWKEKYAAFQRTEGYKYVDPTGLDKYREREILAAVSLENTRQKVAEEISVARSQMLSLE
                     PELNMSGDEKAQMRHELLAEMALEQQQQEEKALRIQQTWVREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            1998..3017
                     /locus_tag="QYN11_RS26790"
                     /old_locus_tag="QYN11_26785"
     CDS             1998..3017
                     /locus_tag="QYN11_RS26790"
                     /old_locus_tag="QYN11_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023148269.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVNQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(3278..3724)
                     /locus_tag="QYN11_RS26795"
                     /old_locus_tag="QYN11_26790"
     CDS             complement(3278..3724)
                     /locus_tag="QYN11_RS26795"
                     /old_locus_tag="QYN11_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_021537414.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLAKDSA"
                     /name="conjugal transfer protein TraD"
     Typing          1999..2773
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          2..1539
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.278"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaagct accattgcac cgttaaaacc ggagcaaaag gcgcagcctt aaaacatgct
       61 gattacattt ccagatccgg cgaatataag agctacaaat cgcgggagga tctggagttt
      121 tcgtcatccg gtaacatgcc atcatgggcg aagaaaaatc ccgcagaact gtggaaagcc
      181 gcagatgaat ttgaacgaaa aaacgggacg gcataccgtg aaattgagat cgcattaccg
      241 cgtgaactga cacgcgaaca acggatagaa cttgttgaag atttcgtgca aaaggaactt
      301 ggcgatcgcc atgcctacca gtacgcgatc cacaatccgc caggtgcaat cgacggaaag
      361 gaacagccac acgcgcatat catgttttgc gagcggatta atgatggtat tgaacgcgat
      421 ccgcagcaat tttttaaacg cgccaacagc aaatcaccgg agcggggagg cgcaaaaaag
      481 gcgagtattc cccagaccgc cggagagcga aaagccgccc ttgttgcgct acgttcccgc
      541 tgggctgatg tgcagaatga gcaccttgcg cgacacggtc acgagtcgcg cgtagatcat
      601 cgttcgctga aagaacaggg catcaagcgc acgccggaag ttcatcttgg ccctgttcac
      661 gccgccaatc tgaacgaggg gcagatcgcg gcaattcagg agcacagggc ggcgcgtcag
      721 caagaacgcg cagcacgcaa cccagcgcca gagcgccaac aggaactgga gcaaaagcaa
      781 aaaatcagga cagttgagcg ttcaccggag ttactggctc agtaccgcaa ggttatgaaa
      841 acggtcattc agggagagga aaggcttgct cgtttagggg atgccaaccc cgatgcgctc
      901 aaagaacata agctgcttca gaatgcgaaa gctaaaaaag aatctctttc cgaatggagc
      961 aggcgaatat atgaaggggc acggtattta gacaaactgg ggcgtaatgt tgtttccgca
     1021 caaagggagc tacgagagtt acaggaacaa cgcaatgctc tgaatgggat tcgtggcctg
     1081 tttcgggggg ctgacaaaag ggagattgat gcgcgtattc tggagcaaga aagcgtgctg
     1141 gaaaccgccg aacgtgaaga gaatgagttc aggtacaagc tacagcaagc tgagtccgag
     1201 tggaaagaga aatacgctgc ttttcagcgt accgagggat ataagtacgt tgatcctacg
     1261 gggctggaca agtacagaga acgtgagatt ctggccgcag tctctttaga gaacaccagg
     1321 caaaaagttg ccgaagaaat atccgttgcc cgtagccaga tgttatctct ggagcctgaa
     1381 cttaatatgt ctggagatga aaaagctcaa atgcgacatg agttactggc agaaatggcg
     1441 ttggaacagc agcaacagga agaaaaagcg ctgcgtatac agcaaacctg ggtcagagag
     1501 gcatctcgta gtaacgaacg tgatcaagat atggagcgtt gaaaatgtag caatatcggg
     1561 actgcgcccc gaaccccgcc aacgtttcac ggaacgcgaa aaaattccgc gtcccgttca
     1621 ccgttttctg atgagaaagt ttgctgtttt gacccgttcg cccttctgaa cgctcgaaac
     1681 agatcgcgca ttgctttcgt gaatagttat gcagcctcct aaaaacaatt ctgccgcgtt
     1741 tttctggttc ttcctggtgt ttccttgtat ttctgcgttt tttgcgtcag aaagcgtctg
     1801 agagcgtttt aaggggggcg cacaacggga gttatggtaa atggatcggt tttgcgggaa
     1861 ggctcgacag gttttgttgt tgggggtagt gtaagcgact gaaaaacaaa cgcccctgaa
     1921 attcgtgtct ctccgccaag attgtcacga gaatacaggg gcgaggtcgg tcgataacct
     1981 ggaaggaatc gtaacacatg agcgccgcgc ttcaatactt cgaagaaaat ttaccccacc
     2041 gcccctatca cacggatgat ctcgctttcg gccttcgcat ctccggcaaa gggcgtgcgc
     2101 ttcttgcgcg gtacatccag cagaaccagc ctcatgcgca gttctggctg gtttttgacg
     2161 ttgaccgcga gggggctgcg attgactgga gcgaccggaa cgcccccgcg ccgaacatca
     2221 ccgttaaaaa tcctgtgaac ggacacgctc acctgctcta cgcactcaac atcgccgtga
     2281 gaaccgcgcc tgatgcctcg gttaaggcgc tgaaatatgc cgcagcggtt gaacgttcgc
     2341 tgtgtgagaa actttgtgct gatgtgaact acagcggcct gatttgcaaa aatccgttcc
     2401 acctggaatg gcaggtgatg gagtggcgcg aggacgccta taccctcgat gaactggcag
     2461 attatctcga tttgagcgcc tcagagcgtc gtagcatcga taaacattac gggatggggc
     2521 gaaactgcca cctgttcgaa atgacgcgca aatgggctta cagggcgatt cgtcagggct
     2581 ggccagcgtt ctcacagtgg cttgaagcgg tgaaccagcg tgtcgaaatg tacaatgcat
     2641 cgcttcccgt tccgctttca ctggctgaat gtcgggctat tggcaagagc attgcgaaat
     2701 acacgcacag gaacttcacg ccggaaactt tcgcacagta tgtggctgat acgcacacgc
     2761 cagaaattca ggcagcacgc ggtcgcaagg gcgggaaggc caattcgtca gaaaatcagt
     2821 cggacaaagg gaaaaaaagc gccgctgttc ggtggactgc taacgacgac aaacgtcgta
     2881 gggcgcttga tatgtatatc ttgggagcgt caacggaaga tattgcagtg gcggttggtg
     2941 ttagcagccg gactattcga cgctggatgg ataattctgg agaatggcta accaagaaac
     3001 aaataattaa atgttagtta acaaagaagc aacaaaaccc cccgtttttt gttaatggtt
     3061 ggttaaattc gcggacatga acaagcctat atcagataac agcgcctttc ggcgtctttt
     3121 tgagcagcgg gctttccggc ggttttctcc tttcagccca gcaatggtgc tgcttaccgc
     3181 atgattgagg tggtcgtgct cgccgcagtc tcatgaccga gcgtagcgag cgaatgagcg
     3241 aggaagcacc agggaaccgg aacgtgaacg gaggctctta cgcactgtct ttggcgagta
     3301 gctctttccc gcgtttttcc cattcctgcc atttgggatg gttgcgcggt agctctgcga
     3361 ttcctgcgag tgcgcctacc agttcggcag cactaaactt tggttttccg gttttgctgt
     3421 ccaccagtcc tgccacaatc atgaggcctg cggccttgta aagttcatga tcccgcgcct
     3481 ttcgttctgc ccttgcggct gccttccgtt ctgccgctat gattgccgtt accctggctt
     3541 ttgctgcggc tgctttctct gcggtttttt ctgcccgtat cagtgcggac aacgtttttt
     3601 catccgtcgt tgttcggttt tttttctctg ccagtaacac aagtaatctc tgctgctctg
     3661 acggtgattt cagtccgttg atgtaggcaa tttttgcact taaccactct tcacgatttg
     3721 tcattcttac ctctgcactt ttgccgttaa tgtgagttgt tattgtgcat aatatcacct
     3781 tgtttgcctg cgtgtgagac cgtaaaatca tccctgatta attcagggat gcgcacttag
     3841 tagtttctcg atttcatcaa gaaactgtgc gctcctgcgg agggctaaaa ggcag
//
