LOCUS       NZ_CP129278             2784 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain G12 plasmid pG12f, complete sequence.
ACCESSION   NZ_CP129278
VERSION     NZ_CP129278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36083486
            Assembly: GCF_030436695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Complete sequence of avian Escherichia coli carrying blaCTX-M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2023) College of Veterinary Medicine, Henan
            Agricultural University, LongZiHu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:28:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,285
            CDSs (total)                      :: 5,162
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 83 of 212
            Pseudo Genes (incomplete)         :: 138 of 212
            Pseudo Genes (internal stop)      :: 45 of 212
            Pseudo Genes (multiple problems)  :: 50 of 212
            Pseudo Genes (short protein)      :: 4 of 212
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129278.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G12"
                     /isolation_source="liver"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pG12f"
                     /geo_loc_name="China:Henan Province"
                     /collection_date="2008-12"
     gene            join(2208..2784,1..8)
                     /locus_tag="QYN12_RS26415"
                     /old_locus_tag="QYN12_26415"
     CDS             join(2208..2784,1..8)
                     /locus_tag="QYN12_RS26415"
                     /old_locus_tag="QYN12_26415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059030.1"
                     /translation="MSKKNNVPMIQNNGNLQPFSMGNLPSIAKTFPMEIHTTILQQLNP
                     LEHISNAISRICYYKEQCKALELESLKIKEQASLMHLKIDTNFKKAMYELETRRKSNEL
                     KLIAAANDFKQISITKSDLIKEISKLVSSICSNDVSLESKKAMNDTLIIMKGMLSDQRS
                     ESYEKFKSLLSSIDSSYNDALLIEDHQDNYV"
                     /name="hypothetical protein"
     gene            1..582
                     /locus_tag="QYN12_RS26420"
                     /old_locus_tag="QYN12_26420"
     CDS             1..582
                     /locus_tag="QYN12_RS26420"
                     /old_locus_tag="QYN12_26420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000481844.1"
                     /translation="MFNIILGVAAVGVGLYILNELDDRAGEEKKRWENQRESVCKSIEE
                     HKRYIEQHVEYVQSMYDFHQLVDLHYSCMKVANEAYKLLNDAKKSMGAIYDAINKLKAH
                     RSKLFNDKKESDDYEKKKEIQAEINMIKEIIDGLYSELDLIKKDKEHFYQELKAFNLKT
                     SSIKYRIRDNTGYKGFDWYNRLQERTLLRR"
                     /name="hypothetical protein"
     gene            1563..2039
                     /locus_tag="QYN12_RS26425"
                     /old_locus_tag="QYN12_26425"
     CDS             1563..2039
                     /locus_tag="QYN12_RS26425"
                     /old_locus_tag="QYN12_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469299.1"
                     /translation="MFIDSEKRLKQLSDEAKKSTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1076..1166
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.398"
                     /coverage="99.0"
                     /evalue="3.45e-27"
     Typing          complement(1377..1485)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(714..964)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.973"
                     /coverage="100.0"
                     /evalue="2.45e-83"
ORIGIN
        1 atgtttaata tcatactcgg agttgctgct gtaggtgttg gtttatatat cttgaacgag
       61 cttgatgatc gcgccggaga agaaaaaaag cgatgggaaa atcaacgaga atcagtatgt
      121 aaaagcattg aagaacataa gagatatata gaacaacatg tcgaatatgt tcaatcaatg
      181 tatgattttc atcagttagt tgaccttcat tattcatgta tgaaagtggc aaatgaagca
      241 tataaacttc ttaatgatgc taaaaaatca atgggcgcta tttatgatgc tatcaataaa
      301 ctcaaagctc accgctctaa attatttaat gataaaaaag aaagcgatga ttatgaaaag
      361 aaaaaagaaa tacaagcaga aataaacatg atcaaagaaa tcatagatgg cctatactct
      421 gaattagatt taattaaaaa ggataaagaa catttttatc aggaattaaa agcttttaac
      481 ttaaaaacta gcagcattaa atacagaatt agagacaata ccggatataa aggctttgat
      541 tggtataatc ggttacaaga acgaacttta cttcgacgtt agttttgcac tgaaactata
      601 tcagataaca atcctccacg gttccggcag cgccttgagc gcaaccggaa accgtctggg
      661 gagaacccca gacccccatc gctgcaacat cgacggtctg cgaccacctg ccaaccgagt
      721 aactgcctgt tccgtgcgaa tgggctttgc agggggctgg cgcggttcct ggcgacctga
      781 aagcccctgg ttaggggtat cggcgcgcct gacctcctga gagcctctgt aacgcatttt
      841 cgcgcctccc atgccctggc gggcatttaa aacccgtttc gtgcgtctga gagcgtttga
      901 agggctatct gacgactcat ccccgaaaga acaacgacgg aatcaccgac aacatgcgag
      961 aggtggcacc gctcgccgca gccccagcga ccgaacgtta gtgagcgagt gggcgaggaa
     1021 gcggaagaga accggtagtt agattgagca cgtacgcact gatgggtgtc ggggcgcagc
     1081 cctgaccaga tggcaaacgg aatatcgtcg tgggtgacgg tattacattt gcacatcctg
     1141 tcccgatttc tgaggcgttt tagccttcat acccctttta gtggtgaacg gacggcaaaa
     1201 acatgatgga actgttgaac gtggagagcg cgaaatcgga caaaaatcac ggctgacgag
     1261 gcttgcagag gagtaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctg
     1321 cgccccgaca cctccaaagg gtgttgcgct caacgcaaca cccttgccta gaatagatct
     1381 tttacgccga tttgtaagtt gttgtttttt tagtgtgttt tttgtttacc tgttgcatcc
     1441 agggtgacac aacgttgtcg catccaggat gcgacaacac gcaacaaggt gttgcacaga
     1501 tgtatcatga tggcttagat ttgaaccaac gttagtgcat gggattttca gagggaaaaa
     1561 tcatgtttat tgattcagaa aaacggctga aacaactttc agatgaggca aagaaaagca
     1621 ccgaggatct cgaagaagca aagaagaatt caaggtttac acaggtatcc ccaaaaggtt
     1681 gggaacgcgt tcgagagctg ctgaaggata gccaaggcat atcagcactg aagctgtact
     1741 catttttagc ggagcatatc gatcctacgt gtggcgctgt cgttgcggat cagcaattcc
     1801 tagctgaaaa acttggagtt agcagaagca caattattcg gtggctcaat tacttagaat
     1861 caaaaaatgc attagttaga atccccgttg ctggtaaggt ttgtgcgtat gccctcgatc
     1921 cacatgaagt ctggaaggga tacaacacta cgaaaaacca tgcagcgttt gtcactaaaa
     1981 cactggtcaa caaagacggt gatattcagc gccgaatcat ggccatgttt tcaaattgag
     2041 ccagcggcag gcggacaatc aggggctacg tgttaacgtt ttgaccatga ttgtctatcc
     2101 tgcattgctc ttttaccacc tagacagcac catagcgata agataaacat gctcaaacgt
     2161 tttatgtaac attagaaaaa ttcagttaat taaaaaaggt tttctaaatg agcaaaaaaa
     2221 ataacgtgcc aatgatacaa aataatggaa atctccaacc atttagcatg ggtaatttac
     2281 catctattgc taaaacattc ccaatggaaa tccatacaac tatacttcaa cagcttaacc
     2341 cattggagca tatatccaat gctataagta ggatttgtta ctataaagaa cagtgtaaag
     2401 cacttgagct tgagtcacta aaaataaaag aacaagccag tttgatgcac cttaaaatag
     2461 atactaattt taaaaaggca atgtatgagc tagaaacaag aagaaagagt aatgagttga
     2521 agttgatagc tgctgcaaat gattttaagc aaatatcaat aacaaaatca gacttaatta
     2581 aagagatatc aaagcttgtt tcaagcatat gtagcaatga cgtttcgttg gaaagtaaaa
     2641 aggcgatgaa cgatactctt atcattatga aaggcatgct cagtgatcaa cggtctgagt
     2701 catatgaaaa attcaaatct ttgttaagta gtattgattc tagttacaat gatgcgttgt
     2761 tgatagagga tcatcaggat aatt
//
