LOCUS       NZ_CP129276             6822 bp    DNA     circular CON 17-JUL-2023
DEFINITION  Escherichia coli strain G12 plasmid pG12d, complete sequence.
ACCESSION   NZ_CP129276
VERSION     NZ_CP129276.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36083486
            Assembly: GCF_030436695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Complete sequence of avian Escherichia coli carrying blaCTX-M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Cui,J. and Yuan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2023) College of Veterinary Medicine, Henan
            Agricultural University, LongZiHu, Zhengzhou, Henan 450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/14/2023 16:28:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,285
            CDSs (total)                      :: 5,162
            Genes (coding)                    :: 4,950
            CDSs (with protein)               :: 4,950
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 83 of 212
            Pseudo Genes (incomplete)         :: 138 of 212
            Pseudo Genes (internal stop)      :: 45 of 212
            Pseudo Genes (multiple problems)  :: 50 of 212
            Pseudo Genes (short protein)      :: 4 of 212
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129276.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G12"
                     /isolation_source="liver"
                     /host="Chicken"
                     /db_xref="taxon:562"
                     /plasmid="pG12d"
                     /geo_loc_name="China:Henan Province"
                     /collection_date="2008-12"
     gene            1..1473
                     /locus_tag="QYN12_RS26325"
                     /old_locus_tag="QYN12_26325"
     CDS             1..1473
                     /locus_tag="QYN12_RS26325"
                     /old_locus_tag="QYN12_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000362089.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(1470..1760)
                     /gene="cki"
                     /locus_tag="QYN12_RS26330"
                     /old_locus_tag="QYN12_26330"
     CDS             complement(1470..1760)
                     /gene="cki"
                     /locus_tag="QYN12_RS26330"
                     /old_locus_tag="QYN12_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            1790..1921
                     /locus_tag="QYN12_RS26335"
                     /old_locus_tag="QYN12_26335"
     CDS             1790..1921
                     /locus_tag="QYN12_RS26335"
                     /old_locus_tag="QYN12_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_113918746.1"
                     /translation="MKIIVFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2116..2259)
                     /locus_tag="QYN12_RS26340"
                     /old_locus_tag="QYN12_26340"
     CDS             complement(2116..2259)
                     /locus_tag="QYN12_RS26340"
                     /old_locus_tag="QYN12_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            2590..2778
                     /locus_tag="QYN12_RS26345"
                     /old_locus_tag="QYN12_26345"
     CDS             2590..2778
                     /locus_tag="QYN12_RS26345"
                     /old_locus_tag="QYN12_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225393024.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWRTFHTILTDAK"
                     /name="hypothetical protein"
     gene            complement(2930..3121)
                     /locus_tag="QYN12_RS26350"
                     /old_locus_tag="QYN12_26350"
     CDS             complement(2930..3121)
                     /locus_tag="QYN12_RS26350"
                     /old_locus_tag="QYN12_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_050863702.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDRA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3189..3512
                     /locus_tag="QYN12_RS26355"
                     /old_locus_tag="QYN12_26355"
     CDS             3189..3512
                     /locus_tag="QYN12_RS26355"
                     /old_locus_tag="QYN12_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3721..>4158
                     /locus_tag="QYN12_RS26360"
                     /old_locus_tag="QYN12_26360"
                     /pseudo=""
     CDS             3721..>4158
                     /locus_tag="QYN12_RS26360"
                     /old_locus_tag="QYN12_26360"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4190..4750
                     /locus_tag="QYN12_RS26365"
                     /old_locus_tag="QYN12_26365"
     CDS             4190..4750
                     /locus_tag="QYN12_RS26365"
                     /old_locus_tag="QYN12_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            4757..4990
                     /locus_tag="QYN12_RS26370"
                     /old_locus_tag="QYN12_26370"
     CDS             4757..4990
                     /locus_tag="QYN12_RS26370"
                     /old_locus_tag="QYN12_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5474..5896)
                     /locus_tag="QYN12_RS26375"
                     /old_locus_tag="QYN12_26375"
     CDS             complement(5474..5896)
                     /locus_tag="QYN12_RS26375"
                     /old_locus_tag="QYN12_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021539380.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6018..6296
                     /locus_tag="QYN12_RS26380"
                     /old_locus_tag="QYN12_26380"
     CDS             6018..6296
                     /locus_tag="QYN12_RS26380"
                     /old_locus_tag="QYN12_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(6288..6578)
                     /locus_tag="QYN12_RS26385"
                     /old_locus_tag="QYN12_26385"
     CDS             complement(6288..6578)
                     /locus_tag="QYN12_RS26385"
                     /old_locus_tag="QYN12_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     Typing          2758..2845
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2188..2747)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.474"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3503..5097
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggataaag tcactgataa ttctccagat gtggagagca cagaatctac tgaggggtca
       61 ttcccaactg ttggggttga tactggcgat acgattacag cgacgcttgc aactggaact
      121 gaaaatgttg gtggaggcgg tggagcattt ggtggggcca gtgaaagttc tgctgcgata
      181 catgcaaccg ctaaatggtc taccgcgcag ttgaaaaaac atcaggctga acaggctgcc
      241 cgtgctgctg cggctgaggc agcattggca aaagcgaaat ctcagcgtga tgccctgact
      301 caacgtctca aggatattgt taatgacgct ttacgtgcta atgccgctcg tagtccatca
      361 gtaactgacc ttgctcatgc caataatatg gcaatgcagg cagaggctga gcgtttgcgc
      421 cttgcgaagg cagagcaaaa agcccgtgaa gaagctgaag cagcagaaaa agcgctccgg
      481 gaagcagaac gccaacgtga tgagattgcc cgccaacagg ctgaaaccgc gcatttgtta
      541 gcaatggcgg aggcagcaga ggctgagaaa aatcgacagg attctcttga tgaagagcat
      601 cgggctgtgg aagtggcaga gaagaagctg gctgaggcta aagctgaact ggcgaaggcc
      661 gaaagcgatg tacagagtaa gcaagcgatt gtttccagag ttgcagggga gcttgaaaac
      721 gctcaaaaaa gtgttgatgt gaaggttact ggatttcctg gatggcgtga tgttcagaaa
      781 aaactggaga gacaattgca ggataagaag aatgaatatt cgtcagtgac gaatgctctt
      841 aattctgcgg ttagcattag agatgctaaa aaaacagatg ttcagaatgc tgagataaaa
      901 ttaaaagaag ctaaggatgc tcttgagaag agtcaggtaa aagactctgt tgatactatg
      961 gttgggtttt atcaatatat aaccgaacaa tatggggaaa aatattccag aatagctcag
     1021 gatttagctg aaaaggcgaa gggtagtaaa tttagtagtg ttgatgaagc tcttgctgca
     1081 tttgaaaagt ataaaaatgt actggataag aaaatcagta aggttgatcg ggatgctatt
     1141 tttaatgcgt tagagtctgt taattatgat gagttatcta agaatttaac gaagatatca
     1201 aagtcgctta aaatcactag tcgagtatct tttctatatg atgttggttc tgattttaaa
     1261 aatgcaatag aaactggtaa ctggcgacct ttgtttgtca cgctggagaa aagtgcggtc
     1321 gatgtaggtg tggcaaaaat tgtagcatta atgtttagtt ttattgttgg tgtgcctctt
     1381 ggtttctggg ggattgcaat tgtcacaggt attgtttctt cttacatagg ggatgatgag
     1441 ttgaacaaac ttaatgagtt gttagggatt taatttctct ttagagtgta atatatgaaa
     1501 cttggcaatc ctactgggat tgccattaat aaataaaaca atgcagataa cttgcataag
     1561 taccagggtt ctttttttaa ctttgtattt tctttgatat atctagatat ggttaattta
     1621 gagtatggat atagcaatac atgtattgat gatataaata taaataacaa aggagtgtta
     1681 tcattgtcgt taaatattaa aataagaatc catggtataa gtgattcagg taaattatgt
     1741 aggtagtatt ttaaatgcat aagtattttt acttttgttg gaaagaaata tgaaaataat
     1801 agtttttcta attacaatat tattcttgtc aggatgtcaa gcaaactata ttcgtgatgt
     1861 gcaaggagga accgttgcac catcttcgtc ttctgaactg acggggatcg cggttcagta
     1921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa
     1981 acgaaaaaac cgcctgggga ggcggtttag tcgaaggtta agtcagttgg ggactgctta
     2041 accgggtaac tggctttagt ggagcacaga taccaaatgc tgtccttcca gtgtagccgc
     2101 tataaggctt caacttcaag agttctgcgt atctaaatcc actatccagt taccaatggc
     2161 tgctgccagt ggcgttaagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2221 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2281 gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga
     2341 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2401 ggagcttccg gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2461 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaagcgcct
     2521 cctgcgcggc ctttttcggg gttttcgttt cttgctcaca tgttctttcc ggcattatcc
     2581 cctgattctg tggataacac tatcaccgct tttgagtggg cagataccgc tcgccgcagc
     2641 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgagcat
     2701 tttctcctta cgcatctgtg cggcatttca cacccggcat ggcgcacttt tcatacaatt
     2761 ctcactgatg ccaaatgatt aagccagtat acactccgct atcgctacgt gactgggtca
     2821 tggctgcgcc ccgacacccg ctaaaacccg ctgacgcgcc ctgacgggct tgtctgctcc
     2881 cggcatccgc tcacaggcca gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     2941 caccgtcatc cccgaagcgt gcgaggcagc tgcggtaaag ctcgtcagcg cggtcgtgaa
     3001 gcgcttcgca gatgtcagcc tggtcgtcgg cgtccagttc attcagtttt tccagcaggg
     3061 tcagcgtctg gcttttgatg aagcgggcca tgttaagggc ggttttttcc tgtttagtca
     3121 cagttctgtt tctccatgtg tggggagtct gtcggaggtt tttaatgata ccggtgaaac
     3181 gcgaaaggat gctcacgata cgggtcacgg atgaggaaca tgcgcggctg ctggcacgtt
     3241 gtgagggaaa acaacttgcg tcatggatgc gtaaggtctg cctgggggca ccgccatcga
     3301 aaacgtcagg tcttccgaca ctggcaccgc cgctgttgcg ccagttcgct tcggtcggca
     3361 ataacctgaa tcagatagcc cggaagataa acagcggaca gtggtcgggg catgaccggg
     3421 ttcatgtggt ggctgcactg atggccatcg gacgtgaact gagtgagctg agggatgagg
     3481 tccgtaaaca gggagaacgg gatgatagtt aagtttcatg ccaggggaaa aggcggtggc
     3541 agtggtccgg ttgattacct gctgggccgg gaacgtagcc gggaaggggc gagagttctg
     3601 cggggtgctc ccgaagaggt gcgggaactc atcgatgcca cgccgtttgc gaaaaaatac
     3661 acctcggggg ttctgtcttt tgcagagcag accctgccac cgggagagcg tgaaagggtg
     3721 atggagagct ttgagcgggt actgatgccc ggtcttgaaa aaaatcagta cagcatcctg
     3781 tgggtggagc accaggacaa ggggcgtctt gagctgaatt ttgtcatccc gaatatggag
     3841 ctggcgagcg gaaaacggct ccagccctat tacgaccggg cggaccgtcc gcgaatcaat
     3901 gcctggcaga cactggtgaa tcaccattac gggctgcacg acccgaacgc accggagaac
     3961 aggcggacac ttgtcacccc gaataatctg ccgaaagcga aacaggaggc cgcagaagcg
     4021 attacgaggg ggctggaggc tctttaccat gccggagcgc taaaaacgcg tcaggatgtc
     4081 actgaggcgc ttacagcggc aggttttgag gtggtgagga caaccaggag cagtatcagt
     4141 atcgctgatc cggagggcgg gcgtaatctc aggctgaaag gggcgctgta tgagcaatct
     4201 tttacaaatg gggacagact tcgagaagaa actgaaagag cgtgccgtct ctacagagaa
     4261 catgctgaac agcgagttca ggaagctcga agcgtctgta gacagggaac tgaacttaaa
     4321 cgggcagaaa atcagagacg ctatcagcgc acacacagca gcggtgaaag aacaactgga
     4381 gacgctgaac agtgccgtga acgcgcagtt cagcgccaca gagaacgaac tggcccggcg
     4441 gcaggaagag ctgctctgga agctggtcaa agggcggata ctgtacccgt cgctgacggc
     4501 gctttgcgtc acgggcggga tttttctggc gtcatggggg ctgattcagt ggcaggaaag
     4561 ccggattgcg gcgaacatcc tggctatcag ggaccaggaa gagacgctgg cgaaactccg
     4621 ggaaaaaaca tggggtgtga cgtatcacga gggcaggaac gggaaatttc tggtgttgcc
     4681 gtccggggtg aagggggaaa acaactggac ggtggagaag aagaacgcag taagactggt
     4741 cagggagtaa gtgagtatga cagagctgga aatgcactta ctgaacgcct tagagcaatt
     4801 gcagcaggac tatacgcagc ggctgaaaga ctgggagagc gccttcacgg atttgcagca
     4861 tatgttcgag ctgacacgac aggagaacgg gcaactgagg gaacagtgcg acgacttgag
     4921 tcagcaggtg cagagattag ccgggcaggt ggaccgcttg agtcggttat ttatcacgaa
     4981 cagcagataa gggatgaaca ggaaaggcag gcacgcctgg aacgggagcg tgcgcggtcc
     5041 tggcatgaag agcaggaacg ggaagcacgg gtatggcgtg gcccgtcaat ggggatgtga
     5101 cggtgagttg ttccgggagg ttgccagatg gtaccggaac gcgttaaaaa cgcatccggg
     5161 cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct ctttgccaga tcgcgctcat
     5221 tcctcagcct catcccgcga tgtctgcgta tgtcatcaac tgaaaagatg gaaaaaaaac
     5281 agtgtcatga ggtttacgca ggcaaaacca gcgttattca catggctgaa tagctatgca
     5341 tacggttaaa atttatcagg tgcgatcgcg gcagcttttc gggtggtttg ttgccatttt
     5401 tacctgtcag cagccgtgat cgcgctgaac gcatttcagc ggtgcgtaca attaagggat
     5461 tatggtaaat cccttactgt ctgccctcgt atccatcgag ataaaccgca gaaatggtgt
     5521 ctgccattag tttcattttt ttcggcgaag gcatatcgct gtttgtgtat tttctgccag
     5581 caaaggttgt tgtgctccgg agtgagtgaa aaaatttttc tgagtggaat tcttttacac
     5641 gttgctgtgc cgtttctgct gatactccgg ctgctttgtc tttcatcccc atatcccgga
     5701 actgctgaaa gaacgggagc atcatacgta tggtgtctgc tttctgggtg tagaagttag
     5761 gatcaaatcc atcatctgaa gcataaacat aatgccgggt atgtcgttcc aggctggtct
     5821 ttggtgtgca tcctgtaaga gctattgttg ctgttatcag aggtatcaga agcagcctgg
     5881 cagcggcaga tctcataaac atctccttgc gtttttaatc ttgatactca gggagttata
     5941 tctccgaaga gtatctctta tcatacgtcg gtttactccc aatgtgattt gatggtcaat
     6001 gagtcaggaa taatcccgtg aaaaaatgtg tgccagtcct gctttttagt gttctttctg
     6061 cttcggttgc cagtcatgcg gacagcaatg tttttcaaca aaaggccgag aagacaaaaa
     6121 acggcattgt tgcggatgtt aatgccagac agaaaatgtt cagtaacaga ctgaagatac
     6181 aacttcagat cgatgagttg agagcagagc tggctggcac cagagatgcc caaaaaagag
     6241 aggatttgcg taaaaaactg gagtctctgg agcggcaaaa gaaatcctta cgctgatggg
     6301 ggcgtatttt ccccggtttc ttccataacc tttttaccgt tcagatattt acgggcgagg
     6361 cgaatgcgtc ttgcctggta taccgccgag taagcctggg tgggattcac accattactg
     6421 atttcactga ccacatgacg ctgatctgcc agtgtcagcg tgtaaacctg tttctggatg
     6481 aggtcacggg ctttgtcatg ctcatatccg gcagcaacaa gggtgtcgag aagtcgtttg
     6541 tctgttttgt tcagtttatg gcggggtgat ttgctcacat cgttctccgt tttttcatga
     6601 tccaacagtt ttatatctca tcctgatttg ctccggtgtt ctgtttcgac gttctcaatg
     6661 cctgcctccc catagtgtga tccttattgc aaaaatgaaa atccatgctc ttgacatgga
     6721 caatgctgag tagtaggttt tcactgtaca taaaaccagt ggttatatgt acagtatgtt
     6781 gtttttaatt tattgtttta attatcaaag aggaatttct tt
//
