LOCUS       NZ_CP129262             7559 bp    DNA     circular CON 13-JUL-2023
DEFINITION  Escherichia coli strain STEC1587 plasmid p_STEC1587_3, complete
            sequence.
ACCESSION   NZ_CP129262
VERSION     NZ_CP129262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36269290
            Assembly: GCF_030413765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Prevalence and Characterization of Shiga toxin-producing Escherichia
            coli isolated from asymptomatic carriers in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Sui,X. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2023) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, NO.155 Changbai Road, Changping District, Beijing
            102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v3.9.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/13/2023 02:10:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,077
            CDSs (total)                      :: 4,954
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 67 of 186
            Pseudo Genes (incomplete)         :: 107 of 186
            Pseudo Genes (internal stop)      :: 49 of 186
            Pseudo Genes (multiple problems)  :: 38 of 186
            Pseudo Genes (short protein)      :: 3 of 186
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129262.1.
            Bacteria and source DNA available from China CDC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC1587"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p_STEC1587_3"
                     /geo_loc_name="China: Guangxi"
                     /collection_date="2021"
     gene            join(6655..7559,1..742)
                     /gene="cka"
                     /locus_tag="QYF42_RS25350"
                     /old_locus_tag="QYF42_25350"
     CDS             join(6655..7559,1..742)
                     /gene="cka"
                     /locus_tag="QYF42_RS25350"
                     /old_locus_tag="QYF42_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(739..1029)
                     /gene="cki"
                     /locus_tag="QYF42_RS25355"
                     /old_locus_tag="QYF42_25355"
     CDS             complement(739..1029)
                     /gene="cki"
                     /locus_tag="QYF42_RS25355"
                     /old_locus_tag="QYF42_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            1059..1190
                     /locus_tag="QYF42_RS25360"
                     /old_locus_tag="QYF42_25360"
     CDS             1059..1190
                     /locus_tag="QYF42_RS25360"
                     /old_locus_tag="QYF42_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2177..2371)
                     /locus_tag="QYF42_RS25365"
                     /old_locus_tag="QYF42_25365"
     CDS             complement(2177..2371)
                     /locus_tag="QYF42_RS25365"
                     /old_locus_tag="QYF42_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041629.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELGNSIQTHFEAQYGTEP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2989..3183
                     /locus_tag="QYF42_RS25370"
                     /old_locus_tag="QYF42_25370"
     CDS             2989..3183
                     /locus_tag="QYF42_RS25370"
                     /old_locus_tag="QYF42_25370"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_116835902.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCRKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            complement(5313..5732)
                     /locus_tag="QYF42_RS25375"
                     /old_locus_tag="QYF42_25375"
     CDS             complement(5313..5732)
                     /locus_tag="QYF42_RS25375"
                     /old_locus_tag="QYF42_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_094315649.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5854..6132
                     /locus_tag="QYF42_RS25380"
                     /old_locus_tag="QYF42_25380"
     CDS             5854..6132
                     /locus_tag="QYF42_RS25380"
                     /old_locus_tag="QYF42_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315650.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQEAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(6124..6414)
                     /locus_tag="QYF42_RS25385"
                     /old_locus_tag="QYF42_25385"
     CDS             complement(6124..6414)
                     /locus_tag="QYF42_RS25385"
                     /old_locus_tag="QYF42_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315653.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTSPSA"
                     /name="hypothetical protein"
     Typing          2010..2097
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(3992..4070)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="87.0"
                     /evalue="1.57e-30"
     Typing          complement(1457..1999)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.157"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttgacgtg aaggtcaccg gatttccggg atggcgtgac gttcagaaaa aactacagcg
       61 tcagcttgaa gctaagcagg ctgagtactc cgcagttgaa aatgaactta agaatgctgt
      121 tagtttcagg gatggtaagg ccgctgaggt taaagaggca gagcagaaat taaaagaagc
      181 tcaggatgct cttgaaaaga gccagataaa agatgctgtt gatactatgg ttggatttta
      241 tcaatatata accgaacaat atggagaaaa atacgcaaaa attgctcagg atttagcgga
      301 aaagtctaaa ggaaagaaaa ttcaaggtgt tgatgaagct ctggcagcat ttgaaaagta
      361 taagaatgtg ctggataaga aattcagtaa ggttgatcgg gatgctattt ttaatgcgtt
      421 agagtctgtt aattatgatg agttatctaa gaatttaacg aagatatcaa agtcgcttaa
      481 aatcaccagt cgagtatctt ttctatatga tgttggttct gattttaaaa atgcaataga
      541 aactggtaac tggcgacctt tgtttgtcac gctggagaaa agtgcggtcg atgtaggtgt
      601 ggcaaaaatt gtggcattaa tgtttagttt tattgttggt gtgcctcttg gtttctgggg
      661 gattgcaatt gtcacaggta ttgtttcttc ttacataggg gatgatgagt tgagcaaact
      721 taatgagtta ttagggattt aatttctctt tagagtgtaa tatatgaaac ttggcaatcc
      781 tactgggatt gccattaata aataaaacaa tgcagataac ttgcataagt accagggttc
      841 tttttttaac tttgtatttt ctttgatata tctagatatg gttaatttag agtatggata
      901 tagcaataca tgtattgatg atataaatat aaataacaaa ggagtgttat cattgtcgtt
      961 aaatattaaa ataagaatcc atggtataag tgattcaggt aaattatgta ggtagtattt
     1021 taaatgcata agtattttta cttttgttgg aaagaaatat gaaaataata atttttctaa
     1081 ttacaatatt attcttgtca ggatgtcaag caaactatat tcgtgatgta caaggaggaa
     1141 ccgttgcacc atcttcgtct tctgaactga cggggatcgc ggttcagtag aaaagatcaa
     1201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc
     1261 gcctggggag gcggtttagt cgaaggttaa gtcagttggg gactgcttaa ccgggtaact
     1321 ggctttagtg gagcacagat accaaatgct gtccttccag tgtagccgct ataaggcttc
     1381 aacttcaaga gttctgcgta tctaaatcca ctatccagtt accaatggct gctgccagtg
     1441 gcgttaagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1561 aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccgg
     1681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaagcgcctc cggcgtggcc
     1801 tttttcgggg ttttcgtttc ttgcttacat cccctgattc tgtggataac actatcactg
     1861 cttttgagtg ggcagatacc gctcgccgca gccgaacgac cgagcgtagc gagtcagtga
     1921 gcgaggaagc ggaaaagcgc ctggacgagc attttctcct tacgcatctg tgcggcattt
     1981 cacacccggc atggtgcact tttcatacaa cccgcactga tgccatatga ttaagccagt
     2041 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgctaaaacc
     2101 cgctgatgcg ccctgacgag cttgtctgct ggcgaaaaca ctcacggaca agccgcgacc
     2161 gctttcgcca gcccctttat ggttccgtgc cgtactgcgc ttcaaagtgt gtctgtatgc
     2221 tgttaccgag ttcttccgcc agttcgtgca gtcgttcgca catggcggcc tgttcgtctg
     2281 catccagtgc atccagtttt tcgagcaacg tcaggctctg gcttttgata aatcgcgcca
     2341 tgttgagcgc ggtctgctgc tgtttgttca cgttctccct tctccgttac tggtgtttac
     2401 gtccggctgt gcgtcacctg gtcatcacgc caggtgacgt cactgaaaat tatcacacaa
     2461 gccccgtcgt gtgtgggctg cgcgacatta acaccccgtg acgcttattt tttgctgaat
     2521 atgtgcgcga ttttctgtct gcgttgtgtg cagttgtcgt cataaacatg aacgttccgc
     2581 cggttcctga tgtgcgtcac tttgacgttt tgacaaagtg acgcacggga ggtgcttgcg
     2641 caaacccgcg aataacggcg tttaccgcgt gattttttta catctgacaa cgtgacccgg
     2701 cgtgatggcc agttctggcc agagacgttc gcggggtgtc ggggcgcagc catgaccggg
     2761 tggcgtttgt ccggtcgtgt gtgcgcggtc cgacaaatgc acatcctgtc ccgtttttca
     2821 cacggttttt attgattacc ggacagacga acccggattg ccatattgcg aaaatcacgt
     2881 taaaacgatt acgagcgtgc agttgggctg atatatcaac agtcatgcca atatgtatta
     2941 acccgacagg ccgctctttt taattccaat aaaaatataa atacgagtat gaatcaggac
     3001 aaagcaattt atttagaaaa taaatatttt tcacccgcaa acgaagctgt cagactggag
     3061 cgtgaaggcg attacattgg agcatggaaa aaatggaatg atgcgcttca ttatgccaaa
     3121 ggagaaaata taggttggtg tatagccaga gctgaatatt gtagaaagac aggccggttt
     3181 taattctggc ggggtttcaa aggagagtcc cctttggcac gtcgacgtat caaaatgcgc
     3241 cagcattttg tatacagtcg gtcacgtgcc tccagaattt accctcaaaa cgcctctgta
     3301 actgacgcat aacaaaagta taaccaagat ataattaaca tataaccaag gtataactaa
     3361 cctggtgtgt atataattaa gatataatta atatataact aacggtttac caacggatcg
     3421 catgtagtag acgctacata aaaaatcctg aatatggagg attcgtcata aagagattcc
     3481 tgtatttacc gttctgatgc aggacagggc aaacactgca aaaatgatga aaaactgatt
     3541 aaccatactg tctgtattct gaatgaatta cgaagcatag cgataaatta aaaaatcccc
     3601 ctggattaca gggggaagcc atcagagttt gagtaatgag aaatattatt ctggagagag
     3661 caccacaaat gcgctctttt ccaatctgcc gtaaccatcg gcaaaagaca atcaggacag
     3721 gcgaaaaaat ccccctatat ggcaaggggg tgtcaatgag gacatggcaa ttgtgagttt
     3781 tgtgtgtgtt ttatgtgttg cagtttgcaa cgtcagaaat ttgccgtagt tcgtcatgag
     3841 agacaacaaa aaaatccccc gtgactggtg gaggccaggg caaggggggt agcaaatgcc
     3901 tgaataattt tcgtagtttt gatataccca tcgttgcaac cgcaacgggg tgaatttgcc
     3961 gcagttgtgc gcgaaaaaca atcctgattc cgggacagga tgtgtaatcg tataaccgcg
     4021 cacgcacggc tatgcgatta ccacctggtc agggctttgc cccgacaccc tcgacaccgt
     4081 tcccgcgata cgatagaaca tcagggcgcg tttccgggta aacgcatgtt aatgaccggg
     4141 aattcattga tcagggaccg gagacgcgct acaaaactcc tgcgcgtttc tggcatcaac
     4201 tggccatggg gatggtttgg cagtagcagc aagcgccaga aacgcgctca aaacgtctct
     4261 gacagcctga acccaccaaa acatataaaa taattgcccg atgcaaaaaa gtgcgttaga
     4321 aagcgtctga gcgcgtttcc ttgccacctg aacgtcatct ctgggattcg tgctcactcc
     4381 atcaaataac aaataaactt caaagtctga taatgggttc cccctgtaat ccaggggatt
     4441 tttttcgctc actcattacc gcacaactcg acatcacgcc ggagacacgc taaaacactc
     4501 ctgcgcgttt ctggcgtctt ttttgttgct gtgtcgctat actcgttgtc agattcgcgc
     4561 tcaatcatca ctgattaatc tgcgtttatc cttcgaccga tcacgctggc attttttgac
     4621 aaccggcgcc catgaaaacg ccagacggga accgtcatcc gcaccctctg acatactggg
     4681 gattaaaaat ccctgtgccg cacagccggc gttgcataaa attccgctct gcggtgagta
     4741 ttgtttaatc aaaaatcccc gcagttcttc cggaaaaacg cgagtctgtt ctcatgcctg
     4801 aaatgcccac acactacgca aaaccaactt tttgctgatt tgctttttaa atcatgtcgt
     4861 tatggctcta aaaatatatt ttcttattca gatttataca aaagcacgtc gtcgacgacc
     4921 ctatggcgac gctatgacga ccctgcttag ggtcagtact gactatctgt agaaaaaaac
     4981 agttaattaa tgtaggggcg gccctagggg cgtgtcgcat ttttttacag agcgatccta
     5041 ggggcgctcc taggatcgtt ctggtctgat atgcagcaat gagtcagaga attacgaact
     5101 gaaaatttac gaaaataagc ctcagggaca gcggacgcgc ccaaaccgat cgccattcag
     5161 tttcatgcat agtcatgcaa cgcgctgaaa acgttcctga cgcatttttc ctgtttaccc
     5221 cggggaaaac atcccttttt acggtttttg cgccagaaac gcgctgagcg cgttttaacg
     5281 gtgcgtacaa ttaagggatt atggtaaatc ccttactgtc tgccctcgta tccatcgaga
     5341 taaaccgcag aaatggtgtc tgccattagt ctggattttt tcggggtaag gtctttgctg
     5401 cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa ttcctggctg
     5461 tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc tttatcttta
     5521 gctcccatat cccagaactg ccgaaaaaaa ggcaccatca tacgtatggt gtctgctttc
     5581 tgggtgtaga agttaggatc aaatccatca tctgaagcat aaacataatg ccgggtatgt
     5641 cgttccaggc tggtctttgg tgtgcatcct gtaagagcta ttgttgctgt tatcagaggt
     5701 atcagaagca gcctggcagc ggcagatctc ataaacatct ccttgcgttt ttaatcttga
     5761 tactcaggga gttatatctc cgaagagtat ctcttatcat acgtcggttt actcccaatg
     5821 tgatttgatg gtcaatgagt caggaataat cccgtgaaaa aatgtgtgcc agtcctgctt
     5881 tttagtgttc tttctgcttc ggttgccagt catgcggaca gcaatgtttt tcaacaagag
     5941 gccgagaaga caaaaaacgg cattgttgcg gatgttaatg ccagacagaa aatgttcagt
     6001 aacagactga agatacaact tcagatcgat gagttgagag cagagctggc tggcaccaga
     6061 gatgcccaaa aaagagagga tttgcgtaaa aaactggagt ctctggagcg gcaaaagaaa
     6121 tccttacgct gatggggacg tattttcccc ggtttcttcc ataacctttt taccgttcag
     6181 atatttacgg gcgaggcgaa tgcgtcttgc ctggtatacc gccgagtaag cctgggtggg
     6241 attcacacca ttactgattt cactgaccac atgacgctga tctgccagtg tcagcgtgta
     6301 aacctgtttc tggatgaggt cacgggcttt gtcatgctca tatccggcag caaccagggt
     6361 gtcgagaagt cgcttgtctg ttttgttcag tttatggcgg ggagatttgc tcacatcgtt
     6421 ctccgttttt tcatgatcca acagttttat atctcatcct gatttgctcc ggtgttctgt
     6481 ttcgacgttc tcaatgtctg cctccttgta gtgtgatctt tattgcaaaa atgaaaatcc
     6541 atgttcttga catgggcaat gctgagtagt aggtttccac tgtacataaa accagtggtt
     6601 atatatacag tacgttgttt ttaatttatt gttttaatta tcaaagagga atttatggct
     6661 aaagaactaa gtggatatgg accaactgct ggtgagtcta tggggggaac aggggcgaat
     6721 ctgaatcagc aaggtggaaa taataatagt aatagtgggg ttcactgggg tggtggctct
     6781 gggcatggta ataatggtgg tcagggaaat tcaaattcat ctggttctac atcaactgtg
     6841 atgaaaaccg gtgagtcata tctgactccg tggggtgatg ttgtcattaa taatgatggc
     6901 cttccggtga tgaatggtat tgttatgaca gaagagaatt caactctggt tgataatcca
     6961 tttggagggg tctccagagt tcttaattct ttaattagtg atatgccatc actttttgca
     7021 gaaagctcag gaaataacaa caataacact gcttcagtta atactgcacc aacgaatgct
     7081 caggtcagtg atatggataa gagttcgaag gtggtgggta atgtaattaa tgaaaaacaa
     7141 aagcagaaga ataaaattgc tacacagatt agtgaaaaac agaagaaaat cgaggagatg
     7201 aaaaaagtat tcaagcatca ttcttatcat ggtatcactg atcttgagag ggatgttgat
     7261 gagttgcaaa aaaaatcaaa tcagcttgat gcagatatta gcaaacttaa ttcttataaa
     7321 aatacattgc aaagtaaaat tggtgatgtg aataaacaga aggaagctga agaaaaggct
     7381 cgtgaaaatg cagaggtcgc tgaacatgaa actcttaatg aagagaaaca ggcggtggcg
     7441 gaagcagaga agcgtttagc tgaggcgaaa gctgaactgg cgaaggctga aagtgatgta
     7501 cagagtaagc aagctactgt ttccagagtt gcaggagagc ttgaaaacgc tcagaagag
//
