LOCUS       NZ_CP129248             6078 bp    DNA     circular CON 13-JUL-2023
DEFINITION  Acinetobacter baumannii strain 13A297n plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP129248
VERSION     NZ_CP129248.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36085765
            Assembly: GCF_030413705.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Bour,M. and Potron,A.
  TITLE     Complete genome of blaNDM-9 producing Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Bour,M. and Potron,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2023) French National Reference Center for
            Antibiotics Resistance, University Hospital of Besancon, 3 boulevard
            Alexandre Fleming, Besancon 25000, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Spades and Trycycler v. 0.5.0
            Assembly Name                     :: de novo
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 280.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/13/2023 01:50:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,038
            CDSs (total)                      :: 3,942
            Genes (coding)                    :: 3,781
            CDSs (with protein)               :: 3,781
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 116 of 161
            Pseudo Genes (incomplete)         :: 40 of 161
            Pseudo Genes (internal stop)      :: 21 of 161
            Pseudo Genes (multiple problems)  :: 14 of 161
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP129248.1.
            Bacteria and source DNA available from French NRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="13A297n"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed2"
                     /geo_loc_name="France: Nancy"
                     /lat_lon="48.64 N 6.15 E"
                     /collection_date="2013"
                     /collected_by="French National Reference Center for
                     Antibiotics Resistance"
     gene            416..1102
                     /locus_tag="QWY26_RS20155"
                     /old_locus_tag="QWY26_20155"
     CDS             416..1102
                     /locus_tag="QWY26_RS20155"
                     /old_locus_tag="QWY26_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            1193..1726
                     /gene="aadB"
                     /locus_tag="QWY26_RS20160"
                     /old_locus_tag="QWY26_20160"
     CDS             1193..1726
                     /gene="aadB"
                     /locus_tag="QWY26_RS20160"
                     /old_locus_tag="QWY26_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(1885..2553)
                     /locus_tag="QWY26_RS20165"
                     /old_locus_tag="QWY26_20165"
     CDS             complement(1885..2553)
                     /locus_tag="QWY26_RS20165"
                     /old_locus_tag="QWY26_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(2686..3171)
                     /locus_tag="QWY26_RS20170"
                     /old_locus_tag="QWY26_20170"
     CDS             complement(2686..3171)
                     /locus_tag="QWY26_RS20170"
                     /old_locus_tag="QWY26_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(3280..5094)
                     /locus_tag="QWY26_RS20175"
                     /old_locus_tag="QWY26_20175"
     CDS             complement(3280..5094)
                     /locus_tag="QWY26_RS20175"
                     /old_locus_tag="QWY26_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5084..5491)
                     /locus_tag="QWY26_RS20180"
                     /old_locus_tag="QWY26_20180"
     CDS             complement(5084..5491)
                     /locus_tag="QWY26_RS20180"
                     /old_locus_tag="QWY26_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             1194..1723
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             1182..1726
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(3284..5094)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtgagatcc cctgtaagcg gtttagcctt gcgactatcg ctacgatgca ccagtgacac
       61 ttattccctc cacatgcaac gcatatcttc cttaaaaaag gcgcacagaa gtagataaag
      121 ctcgaccgtt aggtgcttat ttgatgattt aaactgctga attaacgaag gacttcaaat
      181 tgaggcttgt caaactgttc taattcttta aaatagatag tggtactagc ctttaaggtg
      241 aatatcagta tgagttgcaa gctctgccga tctttcgtta attgatgttg caagcatcaa
      301 ataactgtac ccatcgaatt taaaactaat ggacagattt cgcaagagac ctgtccatta
      361 ttttttttga ttacacctaa gaattaagct gaaactccta ttctttggac tattaatgtc
      421 aaaacttcct agatttcata acaagcacat cgaaaaacgt aagtattcaa atagcctcct
      481 agccaaatct atttcttcta aagtacgttt ctctaataaa acatttttta atgttaattt
      541 gagaggatcc cgcctacgat catgttcatt caaaaatata aaaattgttt tatctgattt
      601 ttcaggagta atcttaaata agaataggtt tacgaatgtt cacttcaaaa agtgcgtatt
      661 ttatgctgct atttttagag attgtcgctt taaaaattgc acatttgaaa aatgcatttt
      721 tattaataca aatacagaaa gcctaaatca ctcgatatta gactgttcta aaaaatataa
      781 tttttacgaa atgaaaattc ctgatgactt aaaagatcaa ctaacagaat atagaaatat
      841 tccaatactg caaaaaaacc gtttacttca tctaaaaggg ggaagaatca ataccgcaac
      901 cttattcatt ctcctatcca aaatatccaa atacaagctg ctaaatggtc ttaaaaaaat
      961 ttatattgag gaacaaagta aagtaaaaat acttagcact ttcaaactcc ttgaagaaat
     1021 aaagaaccta gttgacacta acaccgcatt caaacagaat aatggtgctc tgccccatcc
     1081 aatcgaaagg ttggatagtt aagacaatca ctgggagctc actattggat gtaacaattc
     1141 aattaatagt ctaattactg acattctggg agggcttact atgttaggcc gcatggacac
     1201 aacgcaggtc gcattgatac accaaattct agctgcggca gatgagcgaa atctgccgct
     1261 ctggatcggt gggggctggg cgatcgatgc acggctaggg cgtgtaacac gcaagcacga
     1321 tgatattgat ctgacttttc ccggcgagag gcgcggcgag ctcgaggcaa tagttgaaat
     1381 gctcggcggg cgcgtcacgg aggagttgga ctatggattc ttagcggaga tcggggatga
     1441 gttacttgac tgcgaacctg cttggtgggc agacgaagcg tatgaaatcg cggaggctcc
     1501 gcagggctcg tgcccagagg cggctgaggg tgtcatcgcc gggcggccag tccgttgtaa
     1561 cagctgggag gcgatcatct gggattactt ttactatgcc gatgaagtac caccagtgga
     1621 ctggcctaca aagcacatag agtcctacag gttcgcatgc acctcactcg gggcggaaaa
     1681 ggttgaggtc ttgcgtgccg ctttcaggtc gcgatatgcg gcctaacaat tcgtccaagc
     1741 cgacgccgct ttgcggcgcg gcttaactca ggtgttagga aacactgcta ccgttatcgg
     1801 atttactgct ttactactaa ccttgttaat tcaacatttg caaaatagtc tgacctttcg
     1861 attgtttggg gtgaaattct tatcttaaaa atctaaatat tcttttagat ctaatgcaga
     1921 ttgctcgata aaatcgtatg tattaataca acgacagtaa ataaattttg ttaaaacctc
     1981 taaatcattt tctagattac ggttaaaaat aatcggttca tgatgagcaa tcctgtttct
     2041 taattcacgt aattgatctg tccaatcgta aaaaatttgc ctgttttcaa ctgttgcatt
     2101 aggaaaaata gaattaaaac atccctgcca aatacgttct tcataattag ccataagcat
     2161 cttttgccag aatacaaatt taagctcagg gatcatttta ctaggcgaat ctgggaactt
     2221 ctctttagca ctatcaaaca tacttttagg gctatacctc cctctacggc taaggcttcg
     2281 aataaaagct tcattttcat gccaactatt tgtttcatta cacctaaaag atatttctat
     2341 tgcctgaaca acagcatttc tcagaacaac ttcaaaataa tttaataagt aaaaaaactt
     2401 attggaaatt attaaattac gttcataaag catcaataca tcagattttt catatggttc
     2461 acctttatct ttatagaaat tttcatactt agataagcgt ggtatagata aagcctgctc
     2521 tatcttaagc actgcatcat ttgacaattc caccgtatat accctaatat atagttgtaa
     2581 gtttgagaga ctgcggatct aagatcttcc ctcccaagcg ctaagattca aaaaagcctt
     2641 tcattaacgt gaaaggcttt tttctttttt tggtcaatta aaagcttaca tattgattgt
     2701 aattgaaata ttttcttgag ggacatcaaa ataattagct aatagtctct tagcttcagg
     2761 aatagatatt ttatccagtg ttttagaaga attatcttca tttaatttgg taaatttcag
     2821 ctcattcaaa tcgatattca tctcttctaa tgaactataa gaaactggat tccgattacc
     2881 atcccacata ttgggaatag atatatcagc atattcggag atatttatta tccatctacc
     2941 atctgcatct tcttcttgag attggctgat actagatatt ttgccaacta aaaatgcagt
     3001 gtgatgatca gcctctggtt ctccccaaga ttgtttccta ttttgcacac aaactagata
     3061 atcgcacttt cctgcattat gagcatttag tctccaagac tgactaccac cttcttttaa
     3121 aattctagtc tttccacgag cagttagtac tactacacag ttattattca taacaccacc
     3181 aaaatatata aacaagccta tactacattc aatatagcta aatttaaaca ttagtacaag
     3241 ctaatactaa gaaataaaca ccaacatact agaaaataat tacatcccaa aatccatacc
     3301 tcgatcttgc ttaggtcgag gtttttctat ctcttgttta cgctcattca tctggacgag
     3361 atttttttca atatctcgga tgtttcgagc aaattctgct gcgctttggc tacttctgac
     3421 gagacagaat gctgtgtggt tgagattctc tccagaattt cttcgttgtt ggctgtgata
     3481 aggctcatct ctcggacttt gccattgatc tgctcggtat agcttgctgt acttcgttct
     3541 agctcgtgaa attgcttctg cattgtcgct aatgatttct gtgcttgctg taagctttgc
     3601 tgatcgctct tgattgtctt tgattgtgct gacaaggctt ggataatttt ggtttgcatt
     3661 gtgtctaaag cttgctgttg cttctgcaat tgcacttgtt gttgcattaa ttgttgctgt
     3721 tgttcttcga tgattctcaa taattgtgtt tctaattcgg tcatcgttta ataccccatt
     3781 ggaatcatgt agccgttctg ccattcgcct gcgttcttgt tgatctgaat acataggcgc
     3841 tgtgctttgc tgtctggtcg cttgcatgtg ctgatcgtca aactgcctcc cgatcggttc
     3901 aaatggtcga ccctcatttg cttctcgttg atcacggtat ttagcgtctg catctcgctg
     3961 tacttgtttt tggtgacgag accaagcgag aaaattgcta taagcaaaat aactgctact
     4021 gaaatcagtc ccatattcaa agtggtaagt tttttctgta ccgcttgatt ctgggcttgt
     4081 tgttcttggc tctgtatttg ctcgtttaat tgcgttaaat ggctcaattt cgattgcagc
     4141 aaggtttgat acaggcttaa ctgcttctcg accgtctggg cgttgttttg aatgctctgg
     4201 gctattttct cgtccgactg ttggatagcg gtcgcagtga tattggctct tatgttcaag
     4261 ctgtctctga tagtctgtga cgtttgttcg ataccgctca gaagttgttc ctctgtattc
     4321 ttcaattcgt tgttgtactt cttgtctata ttcgccagta gctctaaagc cttgctcata
     4381 aatctcgccc tctaaacgga tgggacgttt tgcgccctca tatgggtttt caatggatat
     4441 ggagtttttt acttgtcgag tgacattgat ttgattcgat tcaagccatt gcaccaattc
     4501 ttgccgatcg gcaacatcac cattggcaac cgctcggtaa acacgttgat gtaactgcgc
     4561 tttaaaatcc ttaatatctt tgggtaggtt ctttttattt aaaaatagct gtcgatgctc
     4621 aggatcatcg gggtcataca gtgaatgttc ggtattagtg atctgcttaa atagatcgac
     4681 tcgatctaaa tcgactggcg catagaatgg ctgtaggcgt ttgcctgttg aaagctcaac
     4741 attaggaatc acaaaattaa gctcaagcct tgtctcacct gtgtcctgat taattttgtc
     4801 ttggtgctgt acccataaaa tttgatattg gtctttatct agtcccggga ataaacattc
     4861 ctcaaaattt tgcatgattt tctgtttatc ctggtcagat aaatcatgct cataaaagga
     4921 caaacaacca ctggtatatt ttttggcaaa aggactgcta tcaatgagtt gtgctgtcag
     4981 ttctacatca ccattgagca cttgagcatg ttcacgctct cgatcttcac ctaacaaata
     5041 atcgagacag ccttttgata aacctgaacc atgtctaaag aaatcaacga tcatctggac
     5101 gtaactcccg aagctgctga tctatggaaa tcaacaaatg aagtaacttc actttgtcaa
     5161 aagtacctac ccctgcaata tcggtattca ctgctttagc aatctgattc acattgttgc
     5221 caatccgacc aagctctaaa attaatgctc gctctgtctt gctatacgaa gattcaacaa
     5281 cctttgttgc tttaattttt tggtcatctt ctgctttgga attatgaatt ttagtcgctt
     5341 tctctactgc tgcttgtcta agcaactttg caatcgaacc ataaggatta ttggaatcga
     5401 gcaattgata ttctgattct gaaagtctga ctttaaaggt tcgttctcga actgctttgc
     5461 tctgtttgtt ttcttgttcc tgtcctgaca tttcaatcta ccctgctttg gcttttgtaa
     5521 gctttaaatt ggaaaatctt tgatttttct gtttaaagct tttcgggggt atcgggggtg
     5581 aagcccctga tcaaggtcac agactatcac tgtttaacga agttaaatag gggtagctgt
     5641 gtatccttgc ttatttaaaa atggacgagt gaaattcaat aaaaactaat caaatgacac
     5701 ggtatataaa ctctagtagg tattggcatg aatcttgtaa aaacaaatgc gctcaactct
     5761 ttctcaccgt ccccacatcc ctcgctttgc tcgggctgat ggtgaacgtt gagagagttt
     5821 cggcaatact ttccctaaga ggctttgatt taaaaaaatc aaatatcctc ttaagtattg
     5881 ccgattaacc tgtaattggg ctgacggagc cacgcatcgg tcgtatccgc acatggtttt
     5941 gctgaccatg cagacaagga ctaaaggttt tgcaccttct cccacgcgct ccctgtcgaa
     6001 gtatggtggt atctctgcga ggattactcc aacatgcaac ttaaccaacc tatacaacta
     6061 cttccaacgc tccgccta
//
