LOCUS       NZ_CP128976             6756 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Escherichia coli strain TUM1886 plasmid pMTY1886_Col156, complete
            sequence.
ACCESSION   NZ_CP128976
VERSION     NZ_CP128976.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768952
            Assembly: GCF_030389925.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the stepwise acquisition of blaCTX-M-2 and subsequent
            acquisition of either blaIMP-1 or blaIMP-6 in highly conserved
            IncN-pST5 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-NOV-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM1886_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 238x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 07:48:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,819
            Genes (coding)                    :: 5,506
            CDSs (with protein)               :: 5,506
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 313
            CDSs (without protein)            :: 313
            Pseudo Genes (ambiguous residues) :: 1 of 313
            Pseudo Genes (frameshifted)       :: 142 of 313
            Pseudo Genes (incomplete)         :: 201 of 313
            Pseudo Genes (internal stop)      :: 78 of 313
            Pseudo Genes (multiple problems)  :: 89 of 313
            Pseudo Genes (short protein)      :: 3 of 313
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128976.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1886"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1886_Col156"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
                     /collected_by="Toho University School of Medicine"
     gene            join(5459..6756,1..451)
                     /gene="col"
                     /locus_tag="QVM91_RS29660"
                     /old_locus_tag="QVM91_29660"
     CDS             join(5459..6756,1..451)
                     /gene="col"
                     /locus_tag="QVM91_RS29660"
                     /old_locus_tag="QVM91_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E9"
                     /protein_id="WP_121832143.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENHRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKRFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGK
                     ATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSK
                     QFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHI
                     DIHRGQ"
                     /name="col"
     gene            454..711
                     /locus_tag="QVM91_RS29665"
                     /old_locus_tag="QVM91_29665"
     CDS             454..711
                     /locus_tag="QVM91_RS29665"
                     /old_locus_tag="QVM91_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            773..916
                     /locus_tag="QVM91_RS29670"
                     /old_locus_tag="QVM91_29670"
     CDS             773..916
                     /locus_tag="QVM91_RS29670"
                     /old_locus_tag="QVM91_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            1445..2338
                     /locus_tag="QVM91_RS29675"
                     /old_locus_tag="QVM91_29675"
     CDS             1445..2338
                     /locus_tag="QVM91_RS29675"
                     /old_locus_tag="QVM91_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(2554..2829)
                     /locus_tag="QVM91_RS29680"
                     /old_locus_tag="QVM91_29680"
     CDS             complement(2554..2829)
                     /locus_tag="QVM91_RS29680"
                     /old_locus_tag="QVM91_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            3008..5227
                     /gene="mobQ"
                     /locus_tag="QVM91_RS29685"
                     /old_locus_tag="QVM91_29685"
     CDS             3008..5227
                     /gene="mobQ"
                     /locus_tag="QVM91_RS29685"
                     /old_locus_tag="QVM91_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_121832145.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPVNAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITGLG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVTGIHSG
                     LSESHETLARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCEGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKREEQRRQQQRRPGHFRGMSM"
                     /name="mobQ"
     BGC             459..6756
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2834..3007)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.857"
                     /coverage="100.0"
                     /evalue="2.61e-71"
     Typing          complement(3008..3366)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1446..2338
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.275"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3009..5224
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.61"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaacagaag gaaaataaag aaaaggacgc taaggataaa ttagataagg agagtaaacg
       61 gaataagcca gggaaggcga caggtaaagg taaaccagtt ggtgataaat ggctggatga
      121 tgcaggtaaa gattcaggag cgccaattcc agatcgcatt gctgataagt tgcgtgataa
      181 agaatttaaa aacttcgacg attttcggag aaaattctgg gaagaagtgt cgaaagatcc
      241 tgagttaagt aagcaattta atccaggtaa taaaaaacgc ttgagccaag gattagctcc
      301 acgggctagg aataaagata ctgtcggtgg gcgtcgctca tttgagcttc atcacgacaa
      361 gccaatcagt caggatggtg gtgtttatga tatggacaac ctccgtatca ctacgccgaa
      421 acgccatatt gatattcaca gaggtcagta aaaatggaac tgaaaaacag cattagtgat
      481 tacactgaaa ctgaattcaa aaaaattatt gaagacatca tcaattgtga aggtgatgaa
      541 aaaaaacagg atgataacct cgagcatttt ataagtgtta ctgagcatcc tagtggttct
      601 gatctgattt attacccaga aggtaataat gatggtagcc ctgaagctgt tattaaagag
      661 attaaagaat ggcgagctgc taacggtaag tcaggattta aacagggctg aaatatgaat
      721 gccagttgtt taaggatgaa tgactggcat tctttcacaa caaggagtcg ttatgaaaaa
      781 aataacaggg attattttat tgcttcttgc agtcattatt ctggctgcat gtcaggcaaa
      841 ctatatccgg gatgttcagg gcgggaccgt atctccgtca tcaacagctg aagtgaccgg
      901 attagcaacg cagtaacccg aaatcctctt tgacaaaaac aaagcgtgtc aggctgattc
      961 tgatgcgctt ttttttgaaa tgtcacaaaa gttccatgtg ggagatggga tctaaaatcc
     1021 ctgtgcagaa ccttccatcc ggtgggagaa aacttgtcat tttgacctgt tcgccattcg
     1081 gaacggtcga aaccgatcgc gcatcgcttt cgtgcatagt tatgcagcgc cctaaaaacg
     1141 attctgacgc atttttctgg ttcagcctgg tgtttccttg tctttttgcg ttttttgcgt
     1201 cagaacgcgt ctgagagcgt tttaaggggt acgtacaaca ggagttatgg taaatggatc
     1261 gggttttcgg gaaggttcga caggatttgc cgttgggtgt agtgtaagcg actgaaaaac
     1321 aaacgccccg taaatcgtgc tctcaccgcc aagattgatc acgaaattac agggcgccgg
     1381 gttccgcgtt tcccgatggg aaagcgcggt tagttaaact gtgtaccgag agaaatcgta
     1441 tcacatgagc gccgtacttc aacgcttcag ggaaaaatta ccgcacaaac cgtactgtac
     1501 gaacgatttc gcgtacggcg ttcgcattct gccgaaaaac attgccattc ttgcccgttt
     1561 catccagcag aaccagccac atgcactgta ctggcttccc tttgacgtgg accggacggg
     1621 ggcatcaatc gactggagcg accggaattg tccggccccg aacatcaccg taaaaaatcc
     1681 ccgtaacggg cacgcgcatc tgctctacgc gctcgccctt cctgtgagaa ctgcgccgga
     1741 tgcatcggct tcggcgctca gatacgctgc cgctattgag cgtgcgttgt gtgaaaaact
     1801 gggcgcggat gtgaattaca gcggcctgat ctgcaaaaat ccgtgccacc ctgaatggca
     1861 ggaagtggaa tggcgcgagg aaccctacac tctcgacgaa ctggctgatt atctcgattt
     1921 gagcgcctca gcgcgccgta gcgtcgataa aaattacggg ctggggcgaa actaccatct
     1981 gttcgaaaag gtccgtaaat gggcctacag agcgattcgt cagggctggc ctgtattctc
     2041 acaatggctt gatgccgtga tccagcgtgt cgaaatgtac aacgcatcgc ttcccgttcc
     2101 gctttctccg gctgaatgtc gggctattgg caagagcatt gcgaaatata cacacaggaa
     2161 attctcacca gagggatttt ccgctgtaca ggccgctcga ggtcgcaagg gcggaactaa
     2221 atctaagcgc gcagcagttc ctacatcagc acgttcgctg aagccgtggg aggcattagg
     2281 gatcagtcga gcgacgtact accgaaaatt aaaatgtgat ccagatctcg caaaatgaga
     2341 ccagataagc cttatcagat aacagcgccc ttttggcgtc tttttgagca gtgatctttc
     2401 ttgcgatttc ctccgttttg ccccgtaatg gcgtggcttt ccgcatgatt gaggtggtag
     2461 cgctcgccgc agtctcatga ccgagcgtag cgagcgaatg agcgaggaag cgcaaaggcg
     2521 tccggtggtg catgggcact tacgcgccgg ggcttagtgg ttctgcggtt tcgccggtgg
     2581 tctgggtagc ttctccagct cgttaatcag cggttgtagt cggttcacat ccgcctgtct
     2641 tgtgacttcc tttcgcagaa actggagcag gaacgcacgc agttgcgctt cttccggcct
     2701 ccgtaccctc gccagcatgg cagcccccac aatgactttt tgcgccgtgt ccaggctccg
     2761 gctcttcgcc ttcaggcgct gtaatctggc ctcagcttcg gcaatcttct gttcgagtgt
     2821 tctgctcatt tcgtgactcc gtgcgcggtg aaaaatcgta ttttagcgcg tcactggtag
     2881 ttgcaaaact gaactggcat aatgcacgac acatcacgaa gtgcgcactt atacaatctt
     2941 cacttcgttt cgattgtgtg cgccctgcgg ggcttaaaga aaacggcaaa aaggcattac
     3001 ggaagaaatg gcgatttatc atctaagcat gaaaatcatt tcgcgaaaaa acggctacag
     3061 tgccgttgct tctgctgcct accgttccgg ctctgtcata cccgatgatc gtaccggatt
     3121 aacccacgat tacacccgta aacgcggcgt tgatgatgcg gtcattctca cccctgtgaa
     3181 tgcaccgtcc tggtgtggtg accgttccgt tctctggaat gcggtcgaga aagccgaaca
     3241 gcgccggaac tcccagctgg caagggagat tgaactcgcc attccccgtg agatttcccg
     3301 cgaggccgca cgggaggccg ttctcgcttt tgtccgggaa aactttgtca gtcggggcat
     3361 gattgccgat gtggcgttcc atcatatgga ccggaccaac ccccatgcac acatcatgct
     3421 gaccacgaga gctgtcgggg aaacgggatt cgcaggaaag gtcagggact ggaacgaccg
     3481 ggcactcgcc gagacgtggc gcgcatcatg ggctgaccat gcgaacagag cgcttgcgaa
     3541 cgccggctac caggaagaga tagaccatcg ttcatacgag cgtcagggac tggagaaagc
     3601 gccgggcctt cacctcggaa aggctgcctg tgcgatggaa aaacggggca tggaaacaga
     3661 acgcggtgag cagaaccgcc tgattaacag ccttaacctg gaaatacagg tttcccgcac
     3721 aaggctggca gtcatccagg tacaggagaa cgtgcggaaa cgggaactca gcgatgccgc
     3781 acgtcgtgca gcggaagccc ttaatctgac cattcccgcc gcgaatgcct cagcggatac
     3841 cctgcgggaa ttcattgcca cactgccgca ggaatgcggg aacgcatggg agatgacacc
     3901 ggagttcgtg gcgatgaacg ggaaggcaaa cgacatcaaa cgtgagggga atgcgctggt
     3961 gaaagagcgg gatattctcg aaaaagagat gaccggactc agaaaagccc gtccggtagc
     4021 gtccatcctg tcagagcttc ccctgatgac atgggctgaa ccggaatacc gcaaaagaca
     4081 actccgcttc tggaaactcg agaaacagat tgaatctctt ctccgcacct acagggccgt
     4141 gaaagaacgg gacattcccg cccgtcgtca ggcctttgaa acgcagtgga atacgtggat
     4201 ttcgccggga atggcagagc tgaaagaaaa actgtcagca cgggaagcgg agcggcgcag
     4261 ggaggaagcc gaagcggaag cgcgccggaa ggaacaggaa tatgacgcac ggctgaaaca
     4321 ccatgataac catcgcctga gccgtgaaac ggcattagcc ggggttatta cggggctggg
     4381 gcgtgccaga gagccgggaa cgggcaggat aacccgctac atgatgctga gtaacagagc
     4441 cggagaattc acggtatggg gtgatgagct ggcgcattac ccccagagtg ttcatgaccc
     4501 ggtgaatgtt tacctgtcgc caggtggggc cgtgatggtc acgggtatac attcggggct
     4561 gtcagaatct catgagacgc tggcccggcc tgagcgtgtg agaatgtatt ccggtgcgac
     4621 ggtccggcat gtactggaac agatgcgcca ggggtggccc tcttacggtt ttccggcgct
     4681 gccgcatcac tggccggata atttttattt cagcgacgac cgcaggcccg cagcctctcc
     4741 gctgccgtca gcgcaccggg tggacgtcac cgcttatgcg gcaccggagc aactcatgcc
     4801 cgttgtcttt tcgacagagc gaaacagcag gacgctgaat ctgctgttgt gcgaagggcc
     4861 ggaggaaatg cttgtcggtt ttgtgcgcct ggaggacgag ctgcgtcccg ttcttgcgct
     4921 tccgtcgccg gattacagtc atctgatggt aagcatcatc acagagaacg ggatacacct
     4981 ggcaggttac ggtgaggcca taaaccgtga tgctgatact ccgtacccac cggaaccgaa
     5041 gctgatgcag ttcaggctta agggctgtca ggacacgctt tttgctgcca tcaacaaacc
     5101 ggaagagatg ccggattatc tcttccgtca actcggattt aatcagacct ggcatgagtg
     5161 gaagcgggag gaacagcgca ggcaacaaca acgccgtccc gggcacttcc ggggcatgag
     5221 tatgtgatgt ccggggctgc actccggacc ccgccaacac atcacgggcc acaaaatttt
     5281 ttgtggcccg ctctgcgttt tctaagtgtt atccctcctg acttctaaaa aattttccac
     5341 ctgaacttga cagaaaaaac gatgacgggt actttttgat ctgtacataa aaccagtggt
     5401 tttatgtaca gtattaatca tgtaattaat tgttttaacg cttaaaagag ggacttttat
     5461 gagcggtgga gatggacgcg gccataacac gggcgcgcat agcacaagtg gtaacattaa
     5521 tggtggcccg accgggattg gtgtaagtgg tggtgcttct gatggttcag gatggagttc
     5581 ggaaaataac ccgtggggtg gtggttccgg tagcggcatt cactggggag gtggctccgg
     5641 tcgtggtaat ggcgggggta atggcaattc cggtggtggc tcgggaacag gcggtaattt
     5701 gtcagcagta gctgcgccag tggcatttgg ttttccggcg ctttccactc caggagctgg
     5761 cggtctggct gtcagtattt ctgcaagcga attatcggca gctattgctg gtattattgc
     5821 taaattaaaa aaagtaaatc ttaaattcac tccttttggg gttgtcttat cttcattaat
     5881 tccgtcggaa atagcgaaag atgaccccaa tatgatgtca aagattgtga cgtcattacc
     5941 cgcagatgat attactgaat cacctgtcag ttcattacct ctcgataagg caacagtaaa
     6001 cgtaaatgtt cgtgttgttg atgatgtaaa agacgaacga cagaatattt cggttgtttc
     6061 aggtgttccg atgagtgttc cggtggttga tgcaaaacct accgaacgtc caggtgtttt
     6121 tacggcatca attccaggtg cacctgttct gaatatttca gttaataaca gtacgccagc
     6181 agtacagaca ttaagcccag gtgttacaaa taatactgat aaggatgttc gcccggcagg
     6241 atttactcag ggtggtaata ccagggatgc agttattcga ttcccgaagg acagcggtca
     6301 taatgccgta tatgtttcag tgagtgatgt tcttagtcct gaccaggtaa aacaacgtca
     6361 ggatgaagaa aatcaccgtc agcaggaatg ggatgctacg catccggttg aagcggctga
     6421 gcgaaattat gaacgcgcgc gtgcagagct gaatcaggca aatgaagatg ttgccagaaa
     6481 tcaggagcga caggctaaag ctgttcaggt ttataattcg cgtaaaagcg aacttgatgc
     6541 agcgaataaa actcttgctg atgcaatagc tgaaataaaa cgatttaatc gatttgccca
     6601 tgacccaatg gctggcggtc acagaatgtg gcaaatggcc gggcttaaag ctcagcgggc
     6661 gcagacggat gtaaataata agcaggctgc atttgatgct gctgcaaaag agaagtcaga
     6721 tgctgatgct gcattaagtg ccgcgcagga gcgccg
//
