LOCUS       NZ_CP128956             6560 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Escherichia coli strain TUM2805 plasmid pMTY2805_untypable_2,
            complete sequence.
ACCESSION   NZ_CP128956
VERSION     NZ_CP128956.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768984
            Assembly: GCF_030389775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the stepwise acquisition of blaCTX-M-2 and subsequent
            acquisition of either blaIMP-1 or blaIMP-6 in highly conserved
            IncN-pST5 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-NOV-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM2805_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 11x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 07:44:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,867
            Genes (coding)                    :: 4,598
            CDSs (with protein)               :: 4,598
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 87 of 269
            Pseudo Genes (incomplete)         :: 178 of 269
            Pseudo Genes (internal stop)      :: 62 of 269
            Pseudo Genes (multiple problems)  :: 51 of 269
            Pseudo Genes (short protein)      :: 4 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128956.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM2805"
                     /db_xref="taxon:562"
                     /plasmid="pMTY2805_untypable_2"
                     /geo_loc_name="Japan"
                     /collection_date="2006"
                     /collected_by="Toho University School of Medicine"
     gene            complement(123..356)
                     /locus_tag="QVM94_RS24850"
                     /old_locus_tag="QVM94_24850"
     CDS             complement(123..356)
                     /locus_tag="QVM94_RS24850"
                     /old_locus_tag="QVM94_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(363..881)
                     /locus_tag="QVM94_RS24855"
                     /old_locus_tag="QVM94_24855"
     CDS             complement(363..881)
                     /locus_tag="QVM94_RS24855"
                     /old_locus_tag="QVM94_24855"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_052918199.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPCGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<919..1350)
                     /locus_tag="QVM94_RS24860"
                     /old_locus_tag="QVM94_24860"
                     /pseudo=""
     CDS             complement(<919..1350)
                     /locus_tag="QVM94_RS24860"
                     /old_locus_tag="QVM94_24860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1559..1882)
                     /locus_tag="QVM94_RS24865"
                     /old_locus_tag="QVM94_24865"
     CDS             complement(1559..1882)
                     /locus_tag="QVM94_RS24865"
                     /old_locus_tag="QVM94_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956001.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1947..2138
                     /locus_tag="QVM94_RS24870"
                     /old_locus_tag="QVM94_24870"
     CDS             1947..2138
                     /locus_tag="QVM94_RS24870"
                     /old_locus_tag="QVM94_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2806..2919
                     /locus_tag="QVM94_RS24875"
                     /old_locus_tag="QVM94_24875"
     CDS             2806..2919
                     /locus_tag="QVM94_RS24875"
                     /old_locus_tag="QVM94_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            3281..3673
                     /locus_tag="QVM94_RS24880"
                     /old_locus_tag="QVM94_24880"
     CDS             3281..3673
                     /locus_tag="QVM94_RS24880"
                     /old_locus_tag="QVM94_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218243.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRALQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     gene            complement(3892..5463)
                     /locus_tag="QVM94_RS24885"
                     /old_locus_tag="QVM94_24885"
     CDS             complement(3892..5463)
                     /locus_tag="QVM94_RS24885"
                     /old_locus_tag="QVM94_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="WP_000381395.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIETQR
                     PKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLSSQ"
                     /name="IS66-like element ISCro1 family transposase"
     gene            complement(5483..5830)
                     /gene="tnpB"
                     /locus_tag="QVM94_RS24890"
                     /old_locus_tag="QVM94_24890"
     CDS             complement(5483..5830)
                     /gene="tnpB"
                     /locus_tag="QVM94_RS24890"
                     /old_locus_tag="QVM94_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_000624622.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKH
                     PKRTERAGIRI"
                     /name="tnpB"
     gene            complement(5830..6507)
                     /gene="tnpA"
                     /locus_tag="QVM94_RS24895"
                     /old_locus_tag="QVM94_24895"
     CDS             complement(5830..6507)
                     /gene="tnpA"
                     /locus_tag="QVM94_RS24895"
                     /old_locus_tag="QVM94_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="WP_001682408.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTV
                     CSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPMVKKRPRRP
                     NFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLP
                     VTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIKG
                     SSH"
                     /name="tnpA"
     Typing          complement(2224..2311)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2322..2878
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.571"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(20..1569)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccatttttca tggtttcacc atcccagcga agggccatcc agcgtgcgtt cctgtatttc
       61 cggctgacgc tcccgttcca gggataacac atgttcgcgc tcctgtatca accgttcctc
      121 tcttatctcc agttctcgct gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc
      181 tgcacctgct gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta
      241 agcgaaaaca tcttctgcaa ttccacgaag gcgctctccc attcgctcag ccgctgcata
      301 tagtcctgtt gcagctgctc taaggcgttc agcaaatgta tttccagctc tgtcactctg
      361 tgtcactcct tcagatgtac ccactctttc ccctgaaagg gaatcacctc cgctgatttc
      421 ccgcacggaa ggacaaggaa tttcctgttc ccgtcctgca caaactccac gccccatgtc
      481 ttcgcgttca gtttctgcaa tgtctcttcc tgcttcctga tatcttccag gttcgcctgt
      541 atcctcccgc caagatacca gagcgtcccg ccactcgcgg taaacaggag aaagactatc
      601 cccagtaaca tcatgcccgt attccctgcc agctttaaca tgtccttcct gtgctgcatc
      661 atcgcctctt tcaccccttc ccggtgtttt ttcagcgact cctctgtcga ggcggtgaac
      721 agggctatag cgtctttgat tttcgtctcg tttgatgtca cagcctcgct tacagattcg
      781 ccgagcctcc tgaactcgtt gttcagcatt ttctctgtag attcggctct ctctttcagc
      841 tttttctcga actccgcgcc cgtccgtaaa agattgctca taaaacgctc ctttcagtct
      901 gatattcctc ccgccgttcg ggtctgcaat gctgatactg cttcgcgtca ccctgaccac
      961 ttcaagtccc acctctgtga gcgcctgaat cacatcctga cggcctttta tctctccggc
     1021 acggtaaagc acatcaagcc cccgtgtaac gctctcagca agcgcctgtt tcttttccgg
     1081 caggttatca gggagagtca gcgtccggcg gttctccggg gcgttggggt catgcagccc
     1141 gtaatggtga ttcaccagcg tctgccaggc atcaattctc ggcctgtctg cacggtcgta
     1201 gtacggctgg aggcgttttc cggtctgtag ctccatgttc ggaatgacaa aattcagctc
     1261 aagccgtccc ttgtcctggt gctccaccca caggatgctg tactgattct tttcgagacc
     1321 gggcatcagt acacgctcaa aactcgccat cactttttca cgccctcccg gcggcagctc
     1381 cttctccgcg aacgacagaa cacctgacgt gtatttcttc gcaaatggcg tggcatcgat
     1441 gagttcccgg acttcttccg gatcaccctg aagcaccgtt gccccttccc ggttacgctc
     1501 ccttcccagc aggtaatcaa ccggaccact gccaccacct tttcccctgg catgaaactt
     1561 aactatcatc ccgcactccc tgttccctga cggccagacg cagccggcgc agctcatccc
     1621 cgatggccat cagtgcggcc accacctgaa cccggtcacc ggaagaccac tgcccgctgt
     1681 tcaccttacg ggctgtctga ttcaggttat ttccgatggc ggccagctga cgcagtaacg
     1741 gcggtgccag tgtcggcagc ttcccggaac gggcaaccgg ctcacccagg cagacccgcc
     1801 gcatccatac cgccagttgt ttaccctcac agcgttccag taaccgggca tgttcatcat
     1861 cagtaacccg tatcgtgagc atcctctcgc gtttcatcgg tatcattact cccgtgaaca
     1921 gaaatccccc ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg
     1981 cccgctttat cagaagccag acattaacgc tgctggagaa gctcaacgaa ctggacgcag
     2041 atgaacaggc agatatctgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct
     2101 gcctcgcacg tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2161 tcacagcttg tctgtgagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcag
     2221 gttttagcgg gtgtcggggc gcagctctga cccagtcacg tagcgatagc ggagtgtata
     2281 ctggcttaac catgcggcat cagtgcggat tgtatgaaaa gtgcaccatg ccgggtgtga
     2341 aatgccgcac agatgcgtaa ggagaaaatg tagcttccgg cgctcttccg cttcctcgct
     2401 cactgactcg ctacgctcgg tcgttcggct gcggcgagcg gtatcagcgc actcaaagac
     2461 ggtaacacag ttatccacag aatcagggga taaagccaaa agaaacatgt gagcaaaaaa
     2521 caagagcccg aaaaaggccg cgccggtggc gtttttccat aggctccgcc cccctgacga
     2581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac ttaaagatac
     2641 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2761 tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2881 cacgacttaa cgccactggc agcagccatt ggtaactaga caaggtggat tgagatatta
     2941 agagttcttg aagtggtggc ctaactgcgg ctacactaga aggacagtat ttggtatctg
     3001 tgctccacca agccagttac cttcggaaga aagagttggt agctctagat tccggcaaac
     3061 aaaccaccgt tggtagcggt ggtttttttg tttgcaggca gcagattacg cgccgcaaaa
     3121 aaggatctca agaagatctt gaatcactct atgaaaattt tgttttatag ccgtatttgt
     3181 tgatgctata agccagagac cttctatgag aatagacata tcacatcaaa cacggcatac
     3241 cccgccaaac atgcttcctc gggaacaaaa ttgtgtagca atggcattat ctgcgtgctt
     3301 tagacaacaa ctaaaccctg tggtaaacag cttattaaaa gagagaataa tacactcccc
     3361 taaagaattg gaacatgaca acgcagtaat aagagccctg caaaaactac aaattcaaga
     3421 agtttgtaac agcacccttt gggagacagc caaacaacaa ctacttcaaa aatcggacgg
     3481 aagatatttt gccatcaatt ctaaacactt agcctttcca ggcccaggag aatctcacgc
     3541 cttctgttgc atcaaataca aaaatgcaat tggcattaac ggaaacaatg ccgaaaccca
     3601 atcgacccac taccaaccct atccctatga taaagtcagt atatggggac cattccctca
     3661 caatttaacg taatatctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc
     3721 agcagacagg taaaaatggc aacaaaccac ccgaaaaaat gccgcgatcg cgcctgataa
     3781 attttaaccg tatgaatacc tatgcaacca gatggtacag gccacattcc ccccacttaa
     3841 tccactgaag ctgccatttt tgtaagcgac tcgtcagaac cgtattgata tttactgaga
     3901 gctcagatca actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat
     3961 atgttcaatg acgtaacgca gccacttttc tggctccaca ttgttcagac ggcatgtgcc
     4021 gatcagcgag tacaacaccg ccgcatgttc accaccgctg tcggaacccg cgaacatcca
     4081 gtttttccgg cctacggcca ctccccgtaa ggcgttctct gcgatgttgt tgtcgatttc
     4141 cacccagcca ttactgcagt acacgttcag tgcatcccac tgtttcagca ggtatgcgaa
     4201 cgcttttgcc gtatctgagt gacgcgacag tgttttcatc tgttgctgta tccagtcata
     4261 cagtgactgc atcagtggcg cggctctggc ttttcttgcc gccagacgct gttctgctga
     4321 acagccccgg acctctgcct cgatggcata cagttcaccg atacgctgca gggcttccgt
     4381 ggtgatgtag gtgggcgctc ttgcatgcac atcgtggatt tttctccggg catgagccat
     4441 acacgcggct tccgttattc tgccggattc gtataacgcc cggtaaccac cgtaagcatc
     4501 ggcctgaagc acaccgctgt aaccggccag gtgattttgt ggatggatac ctttccggtc
     4561 cggactgtac gcgaaccaga ccgccggggg catctgtgaa ccggcgttac ggtcatcacg
     4621 gacgtagacc cacagccggg ctgtccgggt tttaccgctg cccggctcct ggaccgggac
     4681 ggggatatca tcagcatgga ctttaccggg catcagcaca tactggcgca ggacgtcata
     4741 cagcggctcc agcagttcag caacagcacc tgtccagcgc cccagtgtgg cacggctcag
     4801 ctccactccc tgacgacggt atatttctga ctggcggtat aacggcagat ggtctgcata
     4861 tttcccggtg acaacatggg ccagaagccc cgctccggca taactgcgtg caatgggttt
     4921 tgaaggtact ggtgcctgca cgatatggtc gcaccggcaa caggcctgtt tcggacgttg
     4981 tgtttcgata accttaaagg cgctgctgat aagctccagt tgctctgaca catcacatcc
     5041 cagagaactg agttcaccac cacaggcagg acagcattcc tcttccggcc ggataacccg
     5101 ggtttcacgg ggaagtgagg ccggtaacgg tttacgggct gaagactggc gcagggcgga
     5161 tggcagtacc gggtcatatt gctcacccag cgtttccgcc atttcttcct gaagtgcgct
     5221 gattcgctcc tgtgcttcct gtatctgccg ttcggttttt gcacgaagtt tttctgagct
     5281 tttaccgaac tgcatacgtt gcagtttcgc aaccagcgcc ttcagccggt tgatttcgga
     5341 agcataagcc gccacccgct gtgagagcag gcggttgtat tcagccatct ggcggatggt
     5401 gtcctgttgc gtctgcaaca gtgcccgcag gcgggcgttc tcatgagcaa gtgaggtgtc
     5461 catatcctca ctttacaacg ggttatatgc ggattccagc gcgttccgtt cgtttcgggt
     5521 gcttccagtt gataccttca agaagcatgg ataactgagc cggagtaagg tgcaccttgc
     5581 cgtcacgggt gactggccag acgaagcggc cccgctccag gcgtttggtg aagaggcaca
     5641 gtccgtcact gtcagcccac aacactttta tctggtcacc ccggcgtccg cggaagatga
     5701 acaggtgtcc ggagaacggg tcatccttca ggacgttctg aacttttgat gccaggccgt
     5761 taaagccatt tcgcatatcg gtgatacctg caaccagcca gatacgcgaa cctgcaggga
     5821 gagatatcat cagtggctgc tcccttttat ttcgcggata agtgtctgta ataacgccgg
     5881 cgtcagttta cctttaagcc tgagagttcc ggccggcaga accagctcac aacacagact
     5941 gtcggacggt gtatttatct gctctggttc ctgtgcgggg gccgggattt tattatccgg
     6001 ctccggcgtt aacgtcacgg gaagcagtgc cggcatattt tttccggaag gcagcaggcc
     6061 acctttccgg tattgatggc gccagttgaa gagcaggtta tcgttgattc cgttttcccg
     6121 ggcgatctgc gccacacagg ctccgggctg cagtgactgc tccactaagg cgattttaaa
     6181 ctcataaggg aagttgggcc gccggggacg tttttttacc atgggggctt cggatataac
     6241 ggtgctttca ggacgtacga ctggtaccgt ggaaaattgt ccgtaaaggc aggcatcaag
     6301 ttcctgctcc gacatgcctg cgggcaaagg ccacgaaagg ccagctctcc gaaagcgcac
     6361 gaacatacta caaactgttg attttggtac acccaggcga cgcccggcca caacccgggg
     6421 taaatgttct tcaaagtgaa gacgtaaagc ttcagtgatc caggtccggt gtttcatacg
     6481 atagtgtcca ttaaaaatga tggacattat ttttgtagag ccggaggaaa cagaccagac
     6541 ggtttaaatg agccggttac
//
