LOCUS       NZ_CP128955            13906 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Escherichia coli strain TUM2805 plasmid pMTY2805_Col, complete
            sequence.
ACCESSION   NZ_CP128955
VERSION     NZ_CP128955.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768984
            Assembly: GCF_030389775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the stepwise acquisition of blaCTX-M-2 and subsequent
            acquisition of either blaIMP-1 or blaIMP-6 in highly conserved
            IncN-pST5 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13906)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-NOV-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM2805_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 11x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 07:44:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,987
            CDSs (total)                      :: 4,867
            Genes (coding)                    :: 4,598
            CDSs (with protein)               :: 4,598
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 87 of 269
            Pseudo Genes (incomplete)         :: 178 of 269
            Pseudo Genes (internal stop)      :: 62 of 269
            Pseudo Genes (multiple problems)  :: 51 of 269
            Pseudo Genes (short protein)      :: 4 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128955.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM2805"
                     /db_xref="taxon:562"
                     /plasmid="pMTY2805_Col"
                     /geo_loc_name="Japan"
                     /collection_date="2006"
                     /collected_by="Toho University School of Medicine"
     gene            104..1639
                     /locus_tag="QVM94_RS24775"
                     /old_locus_tag="QVM94_24775"
     CDS             104..1639
                     /locus_tag="QVM94_RS24775"
                     /old_locus_tag="QVM94_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_264832128.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNKQDYLNALKLILSLSAPLMLGLSATSFSFTRI
                     FFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLSFII
                     GVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLLIKV
                     WQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<1732..1996)
                     /locus_tag="QVM94_RS24780"
                     /old_locus_tag="QVM94_24780"
                     /pseudo=""
     CDS             complement(<1732..1996)
                     /locus_tag="QVM94_RS24780"
                     /old_locus_tag="QVM94_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(2141..3017)
                     /locus_tag="QVM94_RS24785"
                     /old_locus_tag="QVM94_24785"
                     /pseudo=""
     CDS             complement(2141..3017)
                     /locus_tag="QVM94_RS24785"
                     /old_locus_tag="QVM94_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3216..3500)
                     /gene="nadS"
                     /locus_tag="QVM94_RS24790"
                     /old_locus_tag="QVM94_24790"
     CDS             complement(3216..3500)
                     /gene="nadS"
                     /locus_tag="QVM94_RS24790"
                     /old_locus_tag="QVM94_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(3502..3834)
                     /locus_tag="QVM94_RS24795"
                     /old_locus_tag="QVM94_24795"
     CDS             complement(3502..3834)
                     /locus_tag="QVM94_RS24795"
                     /old_locus_tag="QVM94_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3979..4215)
                     /locus_tag="QVM94_RS24800"
                     /old_locus_tag="QVM94_24800"
     CDS             complement(3979..4215)
                     /locus_tag="QVM94_RS24800"
                     /old_locus_tag="QVM94_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4611..5824
                     /locus_tag="QVM94_RS24805"
                     /old_locus_tag="QVM94_24805"
     CDS             join(4611..4902,4902..5824)
                     /locus_tag="QVM94_RS24805"
                     /old_locus_tag="QVM94_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_139371347.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQSEYDSQWATICSIAPKIGCTPE
                     TLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGVRP
                     PLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEI
                     QRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAV
                     AAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSSMETTF
                     VLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAES
                     INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGN
                     DDLAA"
                     /name="IS3 family transposase"
     misc_feature    4892..5008
                     /locus_tag="QVM94_RS24805"
                     /old_locus_tag="QVM94_24805"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            5873..6241
                     /locus_tag="QVM94_RS24810"
                     /old_locus_tag="QVM94_24810"
     CDS             5873..6241
                     /locus_tag="QVM94_RS24810"
                     /old_locus_tag="QVM94_24810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(6671..6805)
                     /locus_tag="QVM94_RS24815"
                     /old_locus_tag="QVM94_24815"
     CDS             complement(6671..6805)
                     /locus_tag="QVM94_RS24815"
                     /old_locus_tag="QVM94_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(7989..8306)
                     /locus_tag="QVM94_RS24820"
                     /old_locus_tag="QVM94_24820"
     CDS             complement(7989..8306)
                     /locus_tag="QVM94_RS24820"
                     /old_locus_tag="QVM94_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            9391..9540
                     /locus_tag="QVM94_RS24825"
                     /old_locus_tag="QVM94_24825"
     CDS             9391..9540
                     /locus_tag="QVM94_RS24825"
                     /old_locus_tag="QVM94_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            10000..11228
                     /locus_tag="QVM94_RS24830"
                     /old_locus_tag="QVM94_24830"
     CDS             join(10000..10315,10315..11228)
                     /locus_tag="QVM94_RS24830"
                     /old_locus_tag="QVM94_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            11259..11627
                     /locus_tag="QVM94_RS24835"
                     /old_locus_tag="QVM94_24835"
     CDS             11259..11627
                     /locus_tag="QVM94_RS24835"
                     /old_locus_tag="QVM94_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            11712..12695
                     /locus_tag="QVM94_RS24840"
                     /old_locus_tag="QVM94_24840"
     CDS             11712..12695
                     /locus_tag="QVM94_RS24840"
                     /old_locus_tag="QVM94_24840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(13154..13851)
                     /locus_tag="QVM94_RS24845"
                     /old_locus_tag="QVM94_24845"
     CDS             complement(join(13154..13602,13602..13851))
                     /locus_tag="QVM94_RS24845"
                     /old_locus_tag="QVM94_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          9183..9356
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(6698..7063)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(8708..9216)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cactattggt ggagatgatc ataatgcctt ataatcatac gggatacgaa cttataaagt
       61 cctaaagttt agctaaatcg gaatctacaa cgtagatgat agcttgaaga attatgatgt
      121 cttttatggt ggtatcttta aaaccatcac ggattataaa ttattgaaaa ttcaaaagga
      181 tattaaaaac aatatgactg cactagtcag tagttttaaa tgggttggca ttggacaact
      241 tgtaaaaata gcaacacaat tggtttcgtt attctacttg acgcacttaa tccccccatc
      301 gagctatgga ctattagcaa tgtcatctgt ggttatgaat ttagcaagca tattcaatga
      361 catgggaact ggggcggcag taattcagcg acaagaggcg tcgatcggtt tttataatta
      421 catatacagg ataaacattt ttactgggtt gtttgttatg ctgcttgtaa taataacatc
      481 cccattaatt gtcgtgtact ttaacacacc cgagctatac ccggttctat ttctattggc
      541 ttttagcttt ccaatttcca gcttaacaat aactcacaaa gcaaaattag aaaaagaaat
      601 gaactttaaa gtcatcattg caattgaaat tttctctagc cttacaggaa tggtgctggc
      661 aatagtttta gctaatttag gctttggtgt ttatagtttg atttttcaaa cactgacaac
      721 aatatcaata tccacaatat gttttttgaa ggtttccggg ttgagtttat cattgagaat
      781 taaaaatatc agccatagtg acaagtcagg agttataggg tttagtggtt atttattgtc
      841 ttttaattta ataaattatt tttccaggaa tcttgattct attttaatag gccgtttttt
      901 ctcatccgca atacttgggg tctattcgat tgcctaccgc attatgttat ttccgttgca
      961 gagcctgacg cttgtttttt ctagagtttt cttgccacac cttagcaaaa acataaataa
     1021 cactaataaa caagattact taaatgcatt aaaacttata ctatcattaa gcgcgccact
     1081 gatgctaggt ttatctgcta catctttttc ttttacgagg attttctttg atcctaagtg
     1141 gcacctactt ggagacttat tgcattggtt ggcacctaca gcgattatcc aatcagcctt
     1201 gagtacaaca ggttctgttt ttatggccta cggaaaaaca aggtggttgt ttttcttagg
     1261 ttgcattggc gcttttttga tggggttgtc tttcattata ggtgttcatt tcgatataaa
     1321 aacattggtc tacctttatt tttttgcaaa cttaattaat tcactcccgg catttatttt
     1381 acttaagaat atcttgaagt ttagttacat tgaattggta aacgtgtttt ttagaactat
     1441 ctccccagct tttttgatgt tcttacttat taaagtatgg cagcattttt cctttttcaa
     1501 aaatgccagc attgattttt cttttaatgt cgcattgggt atttttagtt atattgtttt
     1561 ttataccttg tttaatttca atgaggttgt taggacagga gtaattcata agttaagatg
     1621 catgaccaag ttaaagtgat tcatttatta aattcattaa atgaattggt aatgactcca
     1681 acttactgct tgtgttttat gttcagataa tgcccgatac cgacttcggc ctaatttttt
     1741 taagtgtcgt aaaatcgtgt tgaggacagt gcccataatg cgggatgtgg cccggcatcc
     1801 aacgccattc atggccatat caatgatttt ctggtgtgta ccgggctcag aggcggtgta
     1861 agtgaactgt agttgcttgc tatgttttac gacagtgaga gcagagatag cgttgatgtc
     1921 cggtagtgct tttaccgtta cgcaccacgc cttcagtagc tgaacaggag ggataactga
     1981 tggaaatgga agccacggga gcacctcaaa aacaccatta gacactaaac cagcaagttg
     2041 gcatcatcac aaacagttgt tactgctgga aaaaaaacac tatcaacact taagtaatgg
     2101 tgattcaata attaagattc agtatgtaaa atactgaatc ttaattataa attttccttt
     2161 ttctaaaaaa tgtagcgcga ctaataccta aagcaaccca aggctcccct agagacagcc
     2221 tacctcctcc tgatgatttt tcaccaccaa cttttcctct tttggcttgt ataagtgaaa
     2281 aattttgctt attaaatttc aaaaatgtcc acttgcagat acttttcgct attcccgtta
     2341 cttcattctc attcaaaggc actgcaaatt gcagattgta agcgcaggcg cgttcatggc
     2401 aggcttgtag ccactgttcg tactcaggcc agccctgacg gatagcgcga tatgcccact
     2461 ttctggtttt atcaaacagt atgcagttac gacctaagcc ataatcggcg acgatttttt
     2521 tatcgttcgc agcgttcagg tcgagtgatt cggctaacca gtccagcgtg tagagttcag
     2581 gctgccatac gggcaatttt ccagtgactg tggttcggat tcttgcaaat tagccccgaa
     2641 taccccgtgt cagcgcccaa ttttttacgt agcgcgctct ctacagcagc ggcgtaacgt
     2701 aacggtttca tttttccgtc tggcgcggtg cgtatcgagg ttttcagtgc gtacagcagg
     2761 tgcgcgtgtc cgttttcggg gttggtgatg gtcagtgtcg gcgcgggggc gttgcggtcg
     2821 ctccagtcaa tggctgcgcc gatgcggtca acgtcaaatc ccagccagaa catggcgtga
     2881 ggctgattaa actggatgta tttagccaga atagcgcgct ctttccctgc gatgcggaca
     2941 ccaaagtgta aatcatccga gaagtacggt ttatgcggca agcggtcgtt gaaaagttcg
     3001 agtgctgcgt tacgcaatgt gccctgaact tatggcgggc tgttgtctgg cacggttcat
     3061 gtgctaatat ccttcttagg ttttatacct agccctgatt tatcatgtct cttccgccaa
     3121 gttgaaatca tgattatcgg ggctttgttt tttctgttgc cgttaaagtt acacgcgaac
     3181 ggcctcgcct gtcgaatttc ttctgtcaca tcagcttaat gcgccgccag ttcatcaaaa
     3241 aactttgggt tagcctgaat ggtggccagc acttttgctg ccagtccggt tgggttacgg
     3301 cgtccggttt cccagctttt gatggtatcg aggctggttc ccagcacttt cgccatttcg
     3361 ctctgagtaa tattcaggtt ttctctgata gccctgacgt ctgccagctc gtagcgggtc
     3421 acacgggcgg gggctttggt tccgtttttg atttcaacgg cctcttccag agaggctttc
     3481 agctcgtcaa aaaaactcat cttacacctc gtctttcagt cgttgggtca gcttcttcag
     3541 ttctgctttc tctgcgtctg tgatgctgtc tttggtgctt ttagggtagg ccatcaccag
     3601 ataaatcacc tctgcggtgg caaggaagta aatcacccgc gcactgccgc tctttccctg
     3661 attccccgtc gccatcctga tctttcgcag accgcccgtt ttctggatga ggtcaccttt
     3721 gtccggtgac tcgataagct ctttctgaag ctctttgatc tcatcatccg tggcgatttg
     3781 ctttatctga cgggtaaaca tcgttgtctc gataaactca atcgcgtgac tcacggctgt
     3841 ctcctgtata ttttgatgta caatgtacac caagaggcgt gttctgaaaa gccgcgttct
     3901 gtgccatcag acgttacatg gactgacctc gatagcgggt tgcttgcttc tcctgctgta
     3961 actgccgttc ctgctgcttt acgagcgtgt taatcggctc agatcgctgc tcaagcgttc
     4021 gacctgttcg tgcaaggcgt tcacctgatt gctcaagttc agacataccc tgtcgttgcg
     4081 ctccagtgcc tgtgaggtag tctcgaacat gcgttgcaag ctgctgtaac tgtcctgcca
     4141 cgcctgcttc tgctgttcgt agcttgtttg taagctttct aaggcgctca gaagctgcct
     4201 ttccatctcg gtcatggttt ttcctgtcct cagtgataaa aacgtgctgt ggcgtttctg
     4261 tggtgtctga cggggttatt tcgcggtgta cttcatcacg gtgtttccgg ctgtcgatgt
     4321 gtatgtctcg gtgccatgcg ctttcttcgg cagtaccacc agataggtgt tcttctgctt
     4381 gtcgtccttg taaatcgcta cccctgcgcc gctctggggt ccgcttggaa aaaggaaatt
     4441 atcctacgct aagcgcattt tttgcacaga tggctaccga ctgaacgtaa cgtgtgagtc
     4501 atgatcagaa atgtgttaac tctaggaatt gacacgactt actgtttgat ggggaatgaa
     4561 ccgccccggg aatcctggag actaaacttc ctgagaaaga ggtaaacagg atgactaaaa
     4621 atactcgttt ttcccccgaa gtccgtcaac gggcagtccg tatggttctg gaaagtcaga
     4681 gcgaatatga ctcacaatgg gcgacaattt gttccattgc tccaaagatt ggctgtacgc
     4741 cggagactct gcgtgtctgg gttcgccagc atgagcggga taccgggggc ggtgatggag
     4801 ggctcaccac cgctgaacgt cagcgtctga aagagctgga acgtgaaaat cgtgaactgc
     4861 gccgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc
     4921 gcctctggaa aaaatgatgc cactgctgga taagctgcgt gagcagtacg gggtcggacc
     4981 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
     5041 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
     5101 gcgcgtatac gatgaaaatc acaaggtata cggtgtgcgt aaagtctggc gtcagttgtt
     5161 acgggaaggt atcagagtgg ccagatgcac tgtggcacgt ctcatggcgg ttatgggact
     5221 tgccggtgtt ctccggggta aaaaggtccg tacgaccatc agccggaaag ccgttgccgc
     5281 aggcgaccgc gtaaaccgtc agttcgtggc agaacgacct gaccagctgt gggtggctga
     5341 ttttacttac gtcagcacat ggcggggctt cgtctatgtg gcgttcatca ttgatgtgtt
     5401 tgccggatac atcgtggggt ggcgggtctc atcgtccatg gaaacgacat tcgtgctgga
     5461 tgcactggag caggcgttat gggcccgtcg accgtccggc acggtccatc acagtgataa
     5521 aggttctcag tatgtatcgc tggcctacac acagcggctt aaggaagccg gattactggc
     5581 atcaacagga agtacaggcg actcgtatga caacgcgatg gcggagagca tcaatggtct
     5641 ttacaaagcg gaggtaatac accgtaagag ctggaaaaac cgtgcagaag tggaactggc
     5701 cacactcacg tgggtggact ggtataacaa tcgacgattg ctggaaaggc tgggccatac
     5761 tcctccggca gaagcagaaa aagcttatta tgcttccatc ggaaacgatg atctggcagc
     5821 ctgagttcac agataaaaca ctctccagga aacccggggc ggttcagaaa tcatgtttag
     5881 ctatctcaag gctatgtatc atcaatcaaa aattcaggct gaactcaagg cacagataca
     5941 tgaacaaact acggttaatg ctatctgtca tcaccctgaa tcaatcgaga tcattgcggt
     6001 ttgttcgact gatgcgtatt accgaaaacg taaggacgct gcttttctga caacctgttc
     6061 tgtgctcatg cgtactttaa aagatgaatc cgtaccgatg gtactacgga aaacagcatg
     6121 gcgattattg aacgaaagat accaacgaat taagttaaat caggcttata ggatagaaaa
     6181 tttcttgctt gttgccgatt ttgaatatgc tctcgaagaa catgatgaac tagcagagta
     6241 gtactagtga ggcgatgaat accgacagtt aacgtgcatg tgacttaaac gtccgattac
     6301 gtacagcatg tagacaacat gtccgaaagt aagcaaaacc attaacggac agccggatca
     6361 tttcgtacat cccttacggt caggggttcg cgctcaatgg aacgaaatca cccgttagct
     6421 taaaacgctt aacgtaggat aatttccttt ttccaagcgg acccgcaata cagcaaggaa
     6481 cttcttgaga tcctttctgt tgcgtcgtaa tctcttgctc tgtaaacgaa aaaaccaccc
     6541 tggcaggtgg tttttcgaag gttagctaat cctggcagat tttctaaccg cggtaacagt
     6601 cttgtgcgag acatgtcacc aaatctgtcc tttcagtgta gccgcactca ggccatcact
     6661 tcaagaactc ttactgcatc tctcgcacat cctgtttacc aatggccgct gccagtggcg
     6721 tttttgcgtg tctttccgga ttggactcaa gacgatagtt accggacaag acgcagcggt
     6781 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     6841 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac
     6901 cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt
     6961 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattctgtg atgcttgtca
     7021 ggggggcgga gcctatggaa aaccggcagc gccgcggcct ttcctccctc gctttatctc
     7081 gtcttcatcg ccatatgcct ttgtgaatat tctccgtccg ctcgccgcag ccgaacgacc
     7141 gagcgtagcg agtcagtgag cgaggaagcg gaatatcttc ggcctcacgt ccggcacctt
     7201 acactccgta actgcatttt ttcataggat gcaggatagg gctaacgccg caaagtgacg
     7261 tcacctgacg ttcctggatt acaaaggtta aagcagcgga caggaaatgt tttgtgccca
     7321 cagctatgct gggttattca caaaacagcg ggacaaaatg tgtttttgga gtaccgccgg
     7381 cccgctaaag actcctggtt agctcccgca tgtttgctat ctttttgcta ccattttgct
     7441 acttttttgc tatctccaga gtcttctggt ctttcggttg ctatcctttt gctacttttt
     7501 tgctatcaaa atgctacctc tcccttcttg caataaatga ccaaggcacg ttagcgatgt
     7561 atacatcgcc gcgccgcaag ggtgcccctt ccgaatcccg ttcatcactg tgtgctgatt
     7621 aatccaggtt aaatccgacc cccttagcaa taaatcctgc catgcctgac ggcctggcca
     7681 acgcctcacg gtcgcacagt aaactgcgca ccctttcacc gttatcggtg gggttcgtgg
     7741 tggctttcag ggcgctcgcc ggattttagc cgctaaaatg acgtccatgc gttcgtgtgc
     7801 atagccatgc agacgcgaag aaatcatcag gcgcgttttt tcacgatatg gacagggaaa
     7861 gatgccggaa ataggcagga gggcgcagaa atgccctgag ggcgtctgag atggtgcacg
     7921 caacggatgt tatggtaaat tcagctgatt ggcaggtaca aaaaaagccc cgagggcttt
     7981 tttgtacctt attgatctgc acactttgca gccttcaggg cttccagctg atttagccgg
     8041 ttagctgctt ttttgcgggc ttcgcctttt gccatgccat ttcctatccc aaaatcaccc
     8101 aggaaaccta atacggtgcg cccgtcaaat tcgccggttt cctcaatttc ttgttgtaca
     8161 ctgcgcgttt tggcgatttc cagagctacc gctttgcaat ccattgcgga agattctgga
     8221 ccggtgacgt tcacggcctg tggatattgc tttgttgcgc atcctgccat gatcagcgct
     8281 gccgatagga tgaatatttt tttcaaaatt atccttattt atttaggcat agttaagaga
     8341 tacccatttt atcggcagga aatgagggtt ttttagttga attttgggag gggcaaggtt
     8401 ccatattggt cgaggattct ggagctgacc ggcaacggcc agcgcggcta tgggccatgc
     8461 ggtggttgag catagaaagg atcttcttga gttccttttt ttctacgcgt aatctcttgc
     8521 tctgaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttaagtc agttggggaa
     8581 ctgcttaacc tggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8641 tagccgtagt taggccacca cttcaagaac tctatagcac cacctacatt aactcgctct
     8701 gctaatcctg ttaccagtgg ctgctgccag tggcgctttg tcgtgtcttt ccgggttgga
     8761 ctcaagacga tagttaccgg ataaggcgca gcagtcggac tgaacggggg gttcgtgcat
     8821 acagtccagc ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca
     8881 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     8941 cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9001 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     9061 gctttgccgc ggccctatcg cttccctgtt aagtatttcc ctggcatctt ccaggacatc
     9121 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     9181 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgccgc
     9241 cttttttgtc ctgccacatg aagcacttca ctgactccct catcagtgcc aacatagtaa
     9301 gccagtatac actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaaccctct
     9361 aacaacctct cagctactgg atatcacccc atgccatctg aacatgatga acaactggat
     9421 tcgactgata cgcagcttcc cggcgaatat gacctttttg atttaatggc gcaggttact
     9481 gaagagaacc tgcatgccct ggtggatttt gggccgcctg tcggccggga agcgttatag
     9541 ctttttcgct tacccgcgta cgtggtaata cgccggtatt acatcttatt tctttatttt
     9601 catgtaatta caggtggttg taatacgttg cgtattacat aaataaacgg ggacttcagt
     9661 ttttatcttt taattcatgc aattgcgatg aaaatcgcgg ttgcgtatgg tgtatgacca
     9721 tttaagggat aatgtaacct gacttttaaa gaatgaatct atctttggca gcgcatgaga
     9781 tcgataaaaa gcgttccaca tcaggcggtt catgtatcca tgaaccggaa aggacaaaaa
     9841 taagacaatt gtaagacatt ttttgcgttt tactcttatt ttggactatt atgagacaaa
     9901 tgtaagacta gactggcccc ctgaatctcc agacaaccag tatcacttaa ataagtgata
     9961 gtcttaatac tagtttttag actagtcatt ggagaacaga tgattgatgt cttagggccg
    10021 gagaaacgca gacggcgtac cacacaggaa aagatcgcaa ttgttcagca gagctttgaa
    10081 ccggggatga cggtctccct cgttgcccgg caacatggtg tagcagccag ccagttattt
    10141 ctctggcgta agcaatacca ggaaggaagt cttactgctg tcgccgccgg agaacaggtt
    10201 gttcctgcct ctgaacttgc tgccgccatg aagcagatta aagaactcca gcgcctgctc
    10261 ggcaagaaaa cgatggaaaa tgaactcctc aaagaagccg ttgaatatgg acgggcaaaa
    10321 aagtggatag cgcacgcgcc cttattgccc ggggatgggg agtaagctta gtcagccgtt
    10381 gtctccgggt gtcgcgtgcg cagttgcacg tcattctcag acgaaccgat gactggatgg
    10441 atggccgccg cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta
    10501 tcggagagct gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag
    10561 aacttgatgg tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg
    10621 cgctgttgct tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag
    10681 tggccgtgaa agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata
    10741 acggagagag actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact
    10801 gggcggtcac taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg
    10861 tggaacgccg cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg
    10921 gttcatgcta ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga
    10981 agaacacggc ggtgcggagt ccggagagta acggaatagc agagagcttc gtgaaaacga
    11041 taaagcgtga ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc
    11101 ttgcagaggc gttcgagcat tataacgaat ggcatccgca tagtgcgctg ggttatcgct
    11161 cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac agatgtctgg
    11221 aaatataggg gcaaatccaa agacattatg ggcggtatat ggcgaatttt aaccctggtc
    11281 caagaacagc cattgcagct gtcagggatt ccaagaacga tatagaaacc tggaagaaaa
    11341 atggtattcg tagggaagag attgtgactg tgctgaatga acgttacggc ctgaatatct
    11401 cactggttgc gtttgataag gctctttaca gattgagaaa aagtacagat gaaccgtctc
    11461 ttgctgatgt ggattctgtg tctgctgcag aaacgaattt ctctgaagcc agtgggcctg
    11521 acaatggttt ttttctgtcc gggaatgact tcatgcgagc agaagaagaa tctcaaagag
    11581 tggttgagtc attcagagat agccttagca ggaagaaagg attctgattt attcaacaaa
    11641 gccgttgata aatcgcattc cataaataac aacttgatgt tagtataact aaatattgtt
    11701 tggagattac tgtgaaaaaa aaattttcga taatagtacc tgtatataac gttgaaaaat
    11761 acttaaggga atgtattcaa tcagtattag atcaggattt taacgactat gagataatct
    11821 gtgtaaatga tggttcaaca gatggcagtc tagatatcat actgtcattt gatgaccaaa
    11881 gaatcaaagt aataaatcag gaaaataaag gcctgtcggt ggcaagaaat gtcgccattg
    11941 aatcatcaac tggtgaatat atatggtttt tagatagtga tgataaaata tcacatggaa
    12001 gtctaaagtt aatagacaag ataaccaatg aaaaaaatta tgggattatt ttctttaata
    12061 cacatgtttt ttttgacact ccagaactga aaaaaaaagg ctggcatgat tataagcgtt
    12121 caacatttgg tgaaatggat gcattaactt tctttgaaaa agaaatgacg aacaaaaaac
    12181 taatagtgtc agcatgctgc tatgttttta gaagggaatt attaaatggc aatgcattca
    12241 ttccgcatat atatcatgag gataatcact ttatagcaac cctgattttt gacaattatt
    12301 caactatatg cctctcgttg gatgaggcgt tatatcttag gagggtaaga aatgattcca
    12361 taatgacgtc taaaaaatca atgaggcaca tcattggata ttcaacatgc atcgatgagc
    12421 ttacgaagta taaaaaaaca aaaggcatac aaaacaagag ctttgataga tttgtgaatg
    12481 gaatggtttt gtcgttattc tctatagcgg aaacaataaa aacaaaggaa gcaatgaact
    12541 atgtggcgag atataaaaaa caccccgacc tatcatttaa agatagagca ttaattaatt
    12601 acccattaat taaaaagact tatttaaaac taaaaaacgg gaaggtgttt aattacctaa
    12661 ggaatggcat tattaataaa aaaagcaacc cttaataaaa gcaaagcgat aattaataaa
    12721 ggtttttctt tgttgaataa ataagatttc ggataggtat ccccgtgacg ggttgggttt
    12781 tcaggcgata cgcacgcttt ccggggcttt gttgaataaa tcgaactttt gctgagttga
    12841 aggatcagat cggggtcagt ttggagatcg gaaatttttg tacgttaagc gcattattta
    12901 accaatcttg gttcgtaagg ggttgatttt gcgtcagtgg ctatcgcttt taagttgtcg
    12961 ctcttgcagt atcgcattta ggttatccga ttaaggtcaa acctctgaaa atgccgtata
    13021 gcgcgggagt acaccctatt gctataggta agtctgttca aaaaacaggc ttaccgtaca
    13081 ataattctct atatccaaac taaccccaga tcacgcatct tcccgacaac gcagaccggg
    13141 taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg accttgtcat
    13201 gcagctccac cgattttgag aacgacagtg acttccgtcc cagccttgcc agatgttgtc
    13261 tcagattcag attatgtcgc tcaatgcgct gcgtatatcg cttgctgata acgtgcagct
    13321 ttcccttcag gcgtgattca tacagcggcc agccatccgt catccatacc acgacctcaa
    13381 aggccgacag caggctcaga agacgctcca gtgtggccag agtgcgttca ccgaagacgt
    13441 gcgccacaac cgtcctccgt atcctgtcat acgcgtaaaa cagccagcgc tgacgtgatt
    13501 tagcaccgac gtagccccaa tgttcgtcca tttcagcgca gacaatcaca tcacttcccg
    13561 gttgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaaccgtgtt
    13621 gaggccaacg cccataatgc gtgcactggc gcgacatccg acgccattca tggccatatc
    13681 aatgattttc tggtgcgtac cgggctgaga ggcggtgtaa gtgaactgta gttgccatgt
    13741 tttacggcaa tgagagcaga gatagcgctg atgtccggca gtgcttttgc cgttacgcac
    13801 cacgccttca gtagcggagc aggaaggaca tctgatggaa atggaagcca cgcaagcacc
    13861 ttaaaatcac catcatacac taaatcagta agttggcagc attacc
//
