LOCUS       NZ_CP128883             9565 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Escherichia coli strain TUM14759 plasmid pMTY14759_untypable1,
            complete sequence.
ACCESSION   NZ_CP128883
VERSION     NZ_CP128883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35654571
            Assembly: GCF_030389215.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the stepwise acquisition of blaCTX-M-2 and subsequent
            acquisition of either blaIMP-1 or blaIMP-6 in highly conserved
            IncN-pST5 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUN-2023
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM14759_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 61x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 07:44:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,236
            CDSs (total)                      :: 5,117
            Genes (coding)                    :: 4,856
            CDSs (with protein)               :: 4,856
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 146 of 261
            Pseudo Genes (incomplete)         :: 132 of 261
            Pseudo Genes (internal stop)      :: 34 of 261
            Pseudo Genes (multiple problems)  :: 45 of 261
            Pseudo Genes (short protein)      :: 4 of 261
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128883.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM14759"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMTY14759_untypable1"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
                     /collected_by="Toho University School of Medicine"
     gene            join(9006..9565,1..253)
                     /locus_tag="QVN09_RS26045"
                     /old_locus_tag="QVN09_26045"
     CDS             join(9006..9565,1..253)
                     /locus_tag="QVN09_RS26045"
                     /old_locus_tag="QVN09_26045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_289941850.1"
                     /translation="MKIDRILANKKISMVLLIEGTRVLDKSTMPSIRSEDTDGKTYLSK
                     DSVPPSAGSFILGGAIFAAFFSYMVISIQTEDNPLAGPLKAYNYMIYNNMYNSGLSINP
                     IGNGLITENYNLRANEYPIEIIDAKIEKNQIMFKFRIINKTSEALKVSGGWRIYKNFQY
                     ISEHVDTFDYKDEKKREEEEIRVYKKYNVEFVNDNGYLMEKPKERVEVPANSVGYIIIN
                     RKYSPSITYNDLNIEFNFSGGSGEIYQEADMLFTAKNSNISSTILDKK"
                     /name="hypothetical protein"
     gene            complement(493..690)
                     /locus_tag="QVN09_RS26050"
                     /old_locus_tag="QVN09_26050"
     CDS             complement(493..690)
                     /locus_tag="QVN09_RS26050"
                     /old_locus_tag="QVN09_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001298440.1"
                     /translation="MTHQELKVMLLKDPAFRAAYEAESQNPQSGYQIIRHHGDGTEEVV
                     FDSRVSGTDLPTNWMNRPDW"
                     /name="hypothetical protein"
     gene            complement(887..1135)
                     /locus_tag="QVN09_RS26055"
                     /old_locus_tag="QVN09_26055"
     CDS             complement(887..1135)
                     /locus_tag="QVN09_RS26055"
                     /old_locus_tag="QVN09_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001399975.1"
                     /translation="MSVKNKARDRLPGGRLKSYRRVGSHFASCARWFDKSPSWYRNMMM
                     TRPERREVRRLLNQVMRGHDADGIAFPVSHRPFVYWW"
                     /name="hypothetical protein"
     gene            complement(1224..2468)
                     /locus_tag="QVN09_RS26060"
                     /old_locus_tag="QVN09_26060"
     CDS             complement(1224..2468)
                     /locus_tag="QVN09_RS26060"
                     /old_locus_tag="QVN09_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="WP_000722136.1"
                     /translation="MKKILLALTLLFSSCAFAGSELELNKVKLPEAISLIYSEVLKVPY
                     MLDPQLVNDERMITFRLTPDIDEREFVTRYLGNMNIRIWTKKGVDFIAPYTPKEPVKPR
                     YTWTYTPQYRSVAYLSDILGGYVSGSFNNSGAVISDDSLKGSSGASNYINRTGDILVYY
                     GTKEDIAILKTLVTSLDTMSDEVVVSGYVFEVQTSQSDGSGILLAAKILSDKFNISVGA
                     AGLDNFINIRTGSIDAIFNLLKTDSRFTVVSAPRLRVKNNASASFSVGSDVPVLGSVTV
                     NNNTTTQSVEYRSSGVLFNVTPSIKSRTMDLKIQQQLSNFVTTETGVNNSPTLIKRDVT
                     TEVSLADGDIILLGGLAEQKDSKASSGWSFFGSRTSESNKTDIMVMLQVRKVDRSRATP
                     RSAARSGELFRDNLN"
                     /name="type II secretion system protein GspD"
     gene            complement(2468..3493)
                     /locus_tag="QVN09_RS26065"
                     /old_locus_tag="QVN09_26065"
     CDS             complement(2468..3493)
                     /locus_tag="QVN09_RS26065"
                     /old_locus_tag="QVN09_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="WP_001534188.1"
                     /translation="MAISAYIGIPGSGKSYEAVYNVIIPAFTSGRRVVTNIYGLQKDKI
                     TERYPDATGEIIVVDNDDVLKADFFPFKGGEGSFCQFGDLIVIDEAWRIFGSDKDMTAE
                     KKSFIAEHRHFTHPETGISCDLVIVNQSLSNIARFLKDKIETTYRMRKLKALGLNNHYC
                     IDVYSGHKIYKSNLVTSYRNKYNPDIFELYKSYEGNNGNEKQTDKRQSIWNSGKVRFFL
                     VLFPLMFIGSGWLIYSFFSTFGRSDPSPDLTTTDVRDAAMFRSSAATPAPDTPSEPAEP
                     PLSTEWRISGRMTSEGRAFVILVNGAGVLRAVPASSFNYKGMLMSGIIDGERVTLYTGK
                     K"
                     /name="zonular occludens toxin domain-containing protein"
     gene            complement(3497..3784)
                     /locus_tag="QVN09_RS26070"
                     /old_locus_tag="QVN09_26070"
     CDS             complement(3497..3784)
                     /locus_tag="QVN09_RS26070"
                     /old_locus_tag="QVN09_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="WP_000467041.1"
                     /translation="MFGILISALNTLLGFVFRSLIIKFVVFFALYFVVQGFVEILVELL
                     PDSSNLSSLFANLSDGFWYFINLSKLPQGISMIISAMATRFIIRRIPVIG"
                     /name="DUF2523 domain-containing protein"
     gene            complement(3797..5359)
                     /locus_tag="QVN09_RS26075"
                     /old_locus_tag="QVN09_26075"
     CDS             complement(3797..5359)
                     /locus_tag="QVN09_RS26075"
                     /old_locus_tag="QVN09_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000741236.1"
                     /translation="MAARAVIPKVVGRVLVRRFAANDAIYTASQLTATRVFVGRAAANA
                     AEYLPAASSYKMSGVATWAGIAAAVSSFVPSSLSSSDGSVMVMTNGKKISDNLYEVTYS
                     GQSGESKTITVNFEPQELSPVILHVSRNNVDAGSPVVGVETGYSTPDNALYYYQDSKEL
                     IYYYGDNPTEIARNYLNDYNSRTYTETLTNFERTVTNKVVNSNGDVSFTEQNYKFTYPS
                     SFYEIPEITHLYSNPAASSFPAGIPMYENVAGLPMYYSVAYLTAGKQYQYHNTPCKTTN
                     QSNGGYSTICAVPEKEDYTAKDIDEKSELTIWTNTKYKAMTEVLEAGNIESMIDYLEYL
                     DSVSVSPALLADMINELWSEAAVNADYNGLPFKEVSPAEVTSAMSELRLSPTLLDMLSP
                     VSDSAGADVNIDITINNNSGSDTGNNGNIDLGEDPGVKEPELEETPTARDILTPIINLL
                     PFTNEFNIGSRSASCPVVEFSVFNHQYRIDSHCPLIEQNRSAVETIFLIIWGFVALRII
                     LSA"
                     /name="hypothetical protein"
     gene            complement(5574..5807)
                     /locus_tag="QVN09_RS26080"
                     /old_locus_tag="QVN09_26080"
     CDS             complement(5574..5807)
                     /locus_tag="QVN09_RS26080"
                     /old_locus_tag="QVN09_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001534187.1"
                     /translation="MFKKNVNFLAVPALMAVSGATFAAEGAASSGVDLSPLTNSIDFST
                     VLVAIMAVAASLVTLYAGVAGVRWVLRTVKSA"
                     /name="hypothetical protein"
     gene            complement(5842..6093)
                     /locus_tag="QVN09_RS26085"
                     /old_locus_tag="QVN09_26085"
     CDS             complement(5842..6093)
                     /locus_tag="QVN09_RS26085"
                     /old_locus_tag="QVN09_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="WP_000381239.1"
                     /translation="MDTSGFDIQFDNHIPENGYRIEGYLCNANNAKECQAIMVRSEPFH
                     QIDYSAMGNYWTLGFGSVLLLWLFSVGVGQVIKMVRTA"
                     /name="membrane protein"
     gene            complement(6096..6413)
                     /locus_tag="QVN09_RS26090"
                     /old_locus_tag="QVN09_26090"
     CDS             complement(6096..6413)
                     /locus_tag="QVN09_RS26090"
                     /old_locus_tag="QVN09_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WP_000073835.1"
                     /translation="MSNYGLFVKGKMLGARQRNKVNGQGYYNEIGVGLEIPDGFGGTKQ
                     DQIIIRVSQALVNAGVMNQANNFIGKLVQIPVYVRVWSMEGREGVTYNISSDGGITEIK
                     G"
                     /name="DNA-binding protein"
     gene            complement(6425..7477)
                     /locus_tag="QVN09_RS26095"
                     /old_locus_tag="QVN09_26095"
     CDS             complement(6425..7477)
                     /locus_tag="QVN09_RS26095"
                     /old_locus_tag="QVN09_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568107.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_289941848.1"
                     /translation="MIDWFTGILPCTHRPLPAGSVVSVDADGAVEWETVKRLTVRGSHE
                     STMKVRSVGSDGEGRATHLYIDGNPSKFLQGHSVIGSDDLQGLVLTAYARILALLHIPH
                     DLPSYRQVMEGQFKISRIDINYMYSLSTLENVRAWLYAAEFKAKTRHGRACGKGGTVYL
                     GKNSRRWSLKFYSKYDEHTSGKKGHQMADEFVKAGLLDWSKDKLRIELTLRTTELIDLN
                     LTLGNSWNIETPKKLFSDYVGRIEMNQNTILTDEKIINLPRKIQSTYLLWKQGANMKEM
                     LPKPTFYRHRKELLSFGIDINFYCESPDSNNVVPLVRTLEAKPAKIPSWVYEKGLIFDY
                     NRISHASNWH"
                     /name="phage/plasmid replication protein, II/X family"
     gene            7649..8095
                     /locus_tag="QVN09_RS26100"
                     /old_locus_tag="QVN09_26100"
     CDS             7649..8095
                     /locus_tag="QVN09_RS26100"
                     /old_locus_tag="QVN09_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608238.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000527682.1"
                     /translation="MGNNEHEVMADKQRAERIKKVILEHSTYEELAEKTGISVSTLVRI
                     ASGKTEPKFSDIIQIAKITGADLNTLAYGYALDVKEEATERKLITSADGYTDEETTNAH
                     NFIVWNIRTLEKQDILALARQVSALSSYTYSTKMFMKKAVSGEE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            8195..8614
                     /locus_tag="QVN09_RS26105"
                     /old_locus_tag="QVN09_26105"
     CDS             8195..8614
                     /locus_tag="QVN09_RS26105"
                     /old_locus_tag="QVN09_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000089314.1"
                     /translation="MSRNNETSGVELVVVGVFAFCLAVVAWLMKTFDVEWQTALETAPG
                     LIVWLLVVGAGIFFGIKMETGLVRWGAPLAIALLIPVFKPILKEAAGVREMGGLVFDDM
                     VSWYGTGWGMSLMFFGILIVGYGLLYWWHRRNSYY"
                     /name="hypothetical protein"
ORIGIN
        1 taagaaatac aatgtggaat ttgttaatga taatggatat cttatggaaa aaccgaaaga
       61 aagggttgaa gttcctgcaa attcagttgg ctatattata ataaacagaa aatactctcc
      121 aagcattact tataatgacc taaacatcga gtttaacttt agtggtggtt caggtgaaat
      181 ataccaagaa gcagatatgc tttttacagc taagaattct aatataagca gtactatatt
      241 agacaagaaa taattaattg gttcgcacaa tgacgttatg ctaaagaggc cgctgcggag
      301 gccattttcg cagcggcctt ttcaacataa cgagtcgcac attatgcgca ccaatggtaa
      361 ggatggcaga atctggcggg aatcgttgac actggccgtc aactggctac tatcgacagt
      421 gattcaaaat catccgtttc tccaaacgaa tcagaatcaa atgtctgtca gcagtaagtg
      481 aacggcctga cctcaccaat caggcctgtt catccagtta gttggtaaat ctgtacctga
      541 tacacgagaa tcaaacacca cttcttcagt gccatcacca tgatgccgga taatctggta
      601 accggattgc gggttctggc tctcagcttc ataagcagcc cggaatgctg gatctttgag
      661 aagcatcact ttcagctcct gatgtgtcat gaatttttcc atgataagcc ccgatgaatg
      721 ctgccgcgcc gaatcagcca taacgctgaa aatggaaact gtacggcttg gccaaccccg
      781 gacagcaaca aaacaagttt tgtcactgac cggcgttatc tttcaaaaca gacagttaac
      841 gattttttgt ctggtgttta tgcgtgaaat ttcgtgcgta cgaaattcac caccaataaa
      901 caaacggacg atgactgacc ggaaaagcaa tgccgtcagc atcatgacca cgcattacct
      961 gattgagaag ccttctgact tcacgacgct caggcctggt catcatcata ttgcgatacc
     1021 aggacggcga tttatcaaac catctggcac aactggcaaa atgtgaacct acacgacgat
     1081 aagacttaag gcgtccaccg ggaagtcggt cacgcgcctt attttttaca gacataataa
     1141 atcctctaag taactgaata cctagaagat gtcgtactgt tcatacccca taaaagttaa
     1201 cgccataata aaaatccata caatcagttc aggttgtccc ggaacagttc accgctcctc
     1261 gcggcgctgc ggggcgtcgc cctgctccgg tcaacctttc tgacctgaag catcaccata
     1321 atatcagtct tgttgctttc actggtacgg gaaccgaaga agctccagcc ggaactggcc
     1381 ttactgtctt tctgttcagc aaggccacca agtaaaataa tatcaccatc tgcaaggctg
     1441 acttcggttg ttacatcacg cttgataagg gttggcgagt tattgacgcc ggtttcagtg
     1501 gtcacaaagt tggaaagctg ctgctgaatc ttgagatcca ttgtccgact tttgattgat
     1561 ggcgtcacgt taaacaaaac accgctggaa cgatattcaa cggattgcgt tgtcgtattg
     1621 ttattcaccg taacactgcc cagtactggc acatcagagc cgactgaaaa agacgctgag
     1681 gcattatttt ttacccgcag tcgtggcgca ctgacaacag taaaacgact gtcggttttc
     1741 agcagattga aaatggcatc aatggagccg gttcgaatat tgataaaatt atccagtccg
     1801 gcagcaccaa ctgaaatatt gaatttatca gacagaattt tagccgctaa aagaatgccg
     1861 gagccgtcag actgcgaggt ctgaacctca aaaacataac cagaaacaac cacttcatca
     1921 ctcatcgtat ccagcgaagt gaccagtgtt ttcaggatgg cgatatcctc tttcgtacca
     1981 taataaacca gaatatcacc ggttcgattg atgtagttgc ttgcccctga tgaacctttt
     2041 agcgaatcgt cagaaatcac ggctccactg ttattgaagg agcctgaaac gtagccgcca
     2101 agaatatcag acaggtaagc aacagaacgg tactgaggcg tataagtcca tgtataacgt
     2161 ggcttaaccg gctctttcgg cgtatagggc gcgataaaat caacaccctt tttcgtccat
     2221 attcgaatgt tcatattgcc aagataacgg gttacaaatt cccgttcatc aatatcaggt
     2281 gttaacctga atgtaatcat tcgttcatca ttaacaagct gtggatctag catatacggg
     2341 actttaagca cctcactgta aataagagaa atagcctccg gtagcttgac tttatttaat
     2401 tcaagctctg accctgcgaa agcacatgaa gaaaatagta gcgttaatgc gagtaaaatc
     2461 tttttcatta ttttttcccc gtataaagcg tcacacgctc accatcaata attccgctca
     2521 tcaacatccc tttgtaatta aaactggatg caggaacggc acgcaaaaca ccggcaccgt
     2581 taacaagaat cacaaacgcc ctgccttcac tggtcattct ccctgatata cgccactcgg
     2641 ttgaaagtgg cggttcagct ggttctgagg gagtatctgg tgctggagta gcagcggaag
     2701 aacgaaacat ggccgcatca cgtacatctg ttgtagtcaa atctggcgag ggatcgcttc
     2761 ggccaaacgt gctgaaaaat gagtaaatca gccagcctga cccgataaac atcaatggaa
     2821 aaagcacaag aaagaacctg actttgccag aattccagat gctctggcgt ttatctgtct
     2881 gcttttcatt accgttattt ccttcataac ttttgtaaag ttcaaaaata tcagggttat
     2941 atttattgcg ataactggtg acgaggttgc ttttatagat tttgtggcct gaatatacgt
     3001 caatgcaata atgattattc aggcccaacg ctttcagctt gcgcatccgg taagttgttt
     3061 ctattttgtc tttcagaaag cgagcaatat tagaaagtga ctgatttaca ataaccaaat
     3121 cacagctaat acccgtttca gggtgcgtaa aatgacgatg ttcagcaata aatgatttct
     3181 tctcagccgt catatcctta tcgctaccga agattcgcca tgcttcatca ataacaatta
     3241 aatcaccaaa ctggcaaaag ctcccttccc cacctttaaa aggaaagaaa tctgctttaa
     3301 gcacatcatc attatccaca acaataattt ctcccgttgc atcaggataa cgttcggtga
     3361 ttttatcttt ttgtaaacca taaatgttcg tcacaactct ccggccgctg gtaaatgccg
     3421 gaataatgac attgtaaacg gcttcataac tttttcctga gccgggtatg ccaatatatg
     3481 cagaaatagc cataactcac cctataacag gaatacgtcg aataataaaa cgagtagcca
     3541 ttgcggagat aatcatacta atcccctgtg gtaatttact caggttaatg aagtaccaga
     3601 atccgtctga taaattggca aacagcgatg agagattgct tgaatctggc agtaactcaa
     3661 caaggatttc aacgaagccc tggactacaa aatacagcgc aaagaatacg acgaatttaa
     3721 taatcaatga ccggaataca aaacctaaca aagtatttaa tgcactaatc agaatcccaa
     3781 acatagttgc actcctttag gcactcagaa taatacggag cgcaacaaat ccccatataa
     3841 tcagaaatat ggtttctacg gcgcttctgt tctgctcaat taacggacaa tgggagtcaa
     3901 ttctgtactg gtgattaaac acgctgaatt caacaacggg gcaggatgct gaacgagagc
     3961 cgatgttaaa ttcgtttgta aaaggcaaca agttaataat tggcgttaaa atatctcttg
     4021 ccgttggcgt ttcttcaagc tcaggctctt ttacaccggg atcttctccc aaatctatat
     4081 tgccattatt tccagtgtca gatcctgagt tgttattaat ggtaatatca atattgacgt
     4141 cagcccctgc actgtcagat acaggtgaaa gcatatctaa taacgttgga gatagcctaa
     4201 gttccgacat cgcagaagtt acttcagccg gtgaaacctc tttaaacggt aagccgttat
     4261 aatcggcatt aacggcagct tcagaccaca gctcgttaat catgtcggca agaagtgctg
     4321 gcgatacact aacactatca agatactcca gataatcaat catggactca atatttccgg
     4381 cctccagtac ttccgtcatg gctttatatt tggtattggt ccagatagta agctcactct
     4441 tttcatcaat atcttttgcg gtgtaatcct ctttttcagg aacggcgcaa atcgtcgaat
     4501 atccaccatt tgactgatta gtcgttttac aaggcgtgtt gtgatactga tattgctttc
     4561 ctgctgttaa ataagcaacg ctgtaataca tgggaagccc tgctacattt tcatacatgg
     4621 gaatacctgc gggaaatgaa gatgcggccg ggttgctata caagtgtgta atctcaggta
     4681 tttcataaaa tgaggaagga taagtaaact tataattctg ttcagtaaag gatacatctc
     4741 cattactgtt gacaacttta ttcgtcacag tgcgttcaaa attcgttagt gtttcagtat
     4801 aagtacgcga gttatagtca ttaagataat ttctggcaat ttcagttgga ttatcaccat
     4861 agtaataaat tagctctttc gaatcctgat aataataaag tgcattatct ggcgtggaat
     4921 atcctgtttc aacacctaca actggagaac ccgcatccac attattacga ctaacatgaa
     4981 ggattaccgg acttaattcc tgtggttcaa aattaacggt tatcgtctta ctttcaccac
     5041 tttgaccact gtacgttact tcatataaat tatcggaaat ctttttaccg ttagtcatta
     5101 ccattaccga gccgtcagat gaactcaaag aagacggaac aaaagatgat acagcagcag
     5161 caatacccgc ccacgttgca acaccgctca ttttgtatga tgaggcagca ggcaagtatt
     5221 cggcagcatt agccgcagcg cggccaacaa aaacacgggt tgctgtaagc tgtgatgccg
     5281 tataaatcgc atcattggca gcaaagcgac gaacaagcac ccggccaacg actttaggaa
     5341 taacagcccg cgcagccact gctgcaattg ctggtacaaa tgaatatgaa tatcggggaa
     5401 aggaaaccca gagaaaggaa aaaataaagg aataaaccgt cagcctctta gcccaagaat
     5461 aacgacataa gctgagacga tcccccataa aagggaaccc aatttccata attcaatctc
     5521 cataataacc tcaagtaaaa cgggcgatat tgcatcgccc atgaataatg aatttatgcg
     5581 gatttaacgg tacgcaatac ccagcgaacg ccagcgacac cggcataaag tgttaccagt
     5641 gaagcagcaa cggccataat cgcaaccaga actgtactga aatcaatgct gttcgtcagc
     5701 ggcgataaat caacaccact tgatgccgct ccttctgcag cgaaagtcgc gccagaaaca
     5761 gccattaatg caggtacagc cagaaaatta acgttttttt taaacataac tctatctcca
     5821 ttacatttta aaggttcgca ttcaagcagt acgaaccatc tttattacct gtcccacacc
     5881 tacagaaaaa agccagagca gcaggacaga gccaaaacct aatgtccagt aatttcccat
     5941 tgcagaataa tcaatttgat gaaatggttc agaacgtacc attattgcct gacattcttt
     6001 tgcattgttc gcattacata aataaccttc aatacggtaa ccattttcag gaatatgatt
     6061 atcaaactga atatcgaaac ctgatgtatc cataatcagc cttttatttc agtaatgccg
     6121 ccatctgatg aaatgttata agttactcct tccctcccct ccattgacca gacgcgaaca
     6181 tatacaggta tctggactaa cttaccgata aaattatttg cctgattcat tacaccggca
     6241 ttaacaagag cctgagatac acgaataata atctggtctt gcttagtgcc gccaaaacca
     6301 tcaggaatct ctaagccaac accaatttca ttatagtatc cctgaccatt gaccttatta
     6361 cgctggcgag caccaagcat tttacctttt acaaaaagac cataattaga tatatcactc
     6421 tcctttaatg ccagttactg gcatgtgaaa tacggttata atcgaaaatt aaacctttct
     6481 cataaaccca tgatggaatt ttggctggtt tggcttcaag agtacgaacc aacggaacaa
     6541 cgttattaga atccggtgac tcacaataaa agttaatatc tattccgaaa gaaagtaatt
     6601 ctttacggtg tcggtagaat gtaggtttcg gtaacatctc tttcatgttc gcgccctgct
     6661 tccacaataa atatgtagac tgtatttttc ttgggagatt aattattttt tcatcagtta
     6721 atatagtatt ttgattcatt tctattctcc ctacatagtc agagaataat tttttgggcg
     6781 tctcaatatt ccagctatta ccaagcgtaa gattcaaatc aattaattca gttgttctta
     6841 atgttaattc aatgcgtaat ttatcttttg accagtccag taaaccagct ttaacgaatt
     6901 cgtctgccat ctggtgccct tttttgccag atgtatgctc atcatatttt gaataaaatt
     6961 tcaggctcca acgacgggag tttttcccta agtaaacagt gccaccttta ccacaggcgc
     7021 gaccgtggcg agttttagct ttaaattctg cggcatataa ccatgcgcgg acattttcta
     7081 atgttgacaa tgaatacatg taattgatat cgatgcgcga gatcttaaat tgcccttcca
     7141 tgacctgccg ataggatggt agatcatgag gaatatgcag taatgccaat attctggcgt
     7201 aagctgttaa caccaaccct tgcaaatcgt cggaaccgat aacagagtga ccttgcaaaa
     7261 actttgatgg attaccgtca atgtagagat gtgttgcccg accttcacca tcagatccaa
     7321 cagaccttac tttcattgtg gactcatgcg aaccgcgaac ggtcaaccgt tttactgttt
     7381 cccactccac tgcaccgtca gcatcgacgc tgacgacact accagccggt aacggtcggt
     7441 gtgtgcaagg caggattccg gtaaaccagt cgatcataag tagtcacacc catcaaaata
     7501 tgtgtcaata gtcatttgtg agcagattga atggtcacaa tgtaatatgc tcagatctga
     7561 cagtcaatac atttgcaatg tttaaattgt caaaaatgag catcaaatct aactattgaa
     7621 aaaccactgt taagatggag ccaagtagat gggcaacaat gagcatgaag ttatggcgga
     7681 caaacagaga gcagaacgta tcaaaaaggt aattctggaa cactcgactt atgaagaact
     7741 ggccgagaag actggcatta gcgtgagtac gctggtcagg atagcgtccg gcaaaactga
     7801 acctaaattc agtgacatca ttcagatagc taaaattacg ggtgcagact taaacacctt
     7861 agcttatggc tatgctcttg atgttaaaga agaagctacc gagcggaagc taatcacttc
     7921 tgcggatgga tatactgatg aagaaaccac taacgcacat aatttcatcg tgtggaacat
     7981 caggacactg gaaaaacaag atattctggc actcgcaagg caggtttctg ccttaagttc
     8041 atacacttac agtactaaaa tgtttatgaa aaaagcggtg tctggagaag agtaaaagga
     8101 ttaacagtct caggaatgag acaaacgtgc agtattacca acactgcacg ttctttattg
     8161 tgcgaatttt gaacaatgaa aaaggatcaa aatgatgagt agaaataacg aaacaagcgg
     8221 cgttgaattg gttgtcgtgg gcgtatttgc tttctgtctg gcagttgttg cctggttaat
     8281 gaaaactttc gatgttgaat ggcaaacagc actggaaact gcgcctggcc tgatagtctg
     8341 gttgcttgtt gtcggtgcgg ggatcttttt cgggatcaaa atggaaacag gtcttgttcg
     8401 ctggggtgcc ccactggcga tcgcccttct gattccggta ttcaaaccaa ttcttaagga
     8461 agctgccgga gtacgtgaaa tgggtgggtt ggttttcgat gatatggttt cgtggtacgg
     8521 aactggctgg ggaatgtctc tgatgttttt tggaattctt atcgtcggat acggtctgct
     8581 ttattggtgg caccgaagaa attcatatta ttgatataat attaccctag gttaactagg
     8641 gtaatattca aatactaaat ttttacattt tatctgacca tctttgatca gttaaaacac
     8701 tggattttaa gtaaaatgag aaaaaagatg ccggacttga ttactgatac attgaaagta
     8761 gttacttctt tttttgtggc attagtcctc aataagtttt tatctatatt taggaaaaga
     8821 caactttact tatcctgttg gaactccttg agtaaaacct caatatctga caatgcaata
     8881 gtaataaatg catcagtatt taataaaggt aaggataaag aaaaaatgta gaaataaaag
     8941 tcccaaacgg tatgtcttgt actgtaattg caagtgatca tgatgcaact accgaaggtt
     9001 cttttgtgaa gatagataga atattagcaa ataaaaaaat atccatggtg ctcttgattg
     9061 aaggaacaag agttcttgat aaaagtacca tgccttccat aagatcagaa gatactgacg
     9121 ggaaaacata cctttcaaaa gactcagtgc caccgagtgc aggttctttt attttaggcg
     9181 gtgctatttt tgctgcattt ttcagttata tggtcatcag tatccaaaca gaagacaacc
     9241 ctctcgcggg cccattgaaa gcatataact acatgattta taacaatatg tacaattctg
     9301 gactttcgat taatcctata ggtaatggac tgataactga aaattacaat ctaagagcga
     9361 atgaataccc tattgaaata atagatgcca agatagaaaa aaatcaaata atgttcaaat
     9421 ttagaataat taataaaaca tcagaagcac tgaaagtttc aggcggatgg agaatatata
     9481 aaaactttca atatatttcc gaacatgttg atacatttga ttataaagat gaaaagaaaa
     9541 gagaagaaga agaaataagg gtgta
//
