LOCUS       NZ_CP128792             4626 bp    DNA     circular CON 07-JUL-2023
DEFINITION  Escherichia coli strain WL7562 plasmid pWL7562-2, complete sequence.
ACCESSION   NZ_CP128792
VERSION     NZ_CP128792.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35883540
            Assembly: GCF_030388645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Jiang,T.
  TITLE     Complete genome sequence of a carbapenem resistant E. coli isolate
            WL7562
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Jiang,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2023) Department of Clinical Laboratory, The
            Affiliated Wenling Hospital, Chengxi, Wenling 317500, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 07:59:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,873
            CDSs (total)                      :: 4,753
            Genes (coding)                    :: 4,528
            CDSs (with protein)               :: 4,528
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 81 of 225
            Pseudo Genes (incomplete)         :: 145 of 225
            Pseudo Genes (internal stop)      :: 47 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            Pseudo Genes (short protein)      :: 4 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128792.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WL7562"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pWL7562-2"
                     /geo_loc_name="China: Wenling"
                     /collection_date="2021"
                     /collected_by="JT"
     gene            1401..1787
                     /locus_tag="QUY19_RS24320"
                     /old_locus_tag="QUY19_24320"
     CDS             1401..1787
                     /locus_tag="QUY19_RS24320"
                     /old_locus_tag="QUY19_24320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001598756.1"
                     /translation="MRAFNYSPRYANAVRVLNRLLDKEFLSAEEIRLYDDQGRVIEYIN
                     NRLLVPVLSMKISRDGEYVRVWYLSQEAIDDYYNRREYQFSQQRHAVAVARQHRKASTA
                     ARLLLSLGETVPESIRMILAANDD"
                     /name="hypothetical protein"
     gene            2147..2329
                     /locus_tag="QUY19_RS24325"
                     /old_locus_tag="QUY19_24325"
     CDS             2147..2329
                     /locus_tag="QUY19_RS24325"
                     /old_locus_tag="QUY19_24325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087523613.1"
                     /translation="MSKELSLNRISNRVSSSLKEGVVHERVVHGNVYDLELELLRRKRE
                     EAIKRIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            2384..3967
                     /locus_tag="QUY19_RS24330"
                     /old_locus_tag="QUY19_24330"
     CDS             2384..3967
                     /locus_tag="QUY19_RS24330"
                     /old_locus_tag="QUY19_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951932.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_223351761.1"
                     /translation="MSSANEDISGRDSIDQITSRQSMDIIIGFCGAVGSGIKSIVDVTE
                     EELEDLGYEVYRIRVSDLMQKLFSEWCGDTSTPFKRYNSLQDFGDSLRCKYYPHILAEA
                     VIHEIKIEKNLEGKSKELKKRAFLIDQLKHQDEIELLRMVYQHNFYLLGVIRSETERKR
                     NLRDEGLSKSEVDIIIHHDRKSEHPYGQQTAKAILDADYFVKNNQGQRAHLKSKVKRFL
                     GLIHGLNGLTPNAHEKGMYAAHSASLQSACLSRQVGAAILDIKGNLIAVGRNDVPKFNG
                     GLYSADDGVNDHRCVYKGGKCYNEVRKLKIKDKIQQILLSDKVKELSVQLTQEQAEKVA
                     GIIYEGTPVSSIIEYSRSIHAEMDAITSLARLGNGGFEDKVLYTTTFPCHNCARHIVAV
                     GINKVVYIEPYEKSLALELHDDAITEVNESGKVLFESFEGVSPRRYHKFFFSTDERKDS
                     KGNAEKYSTKYKNHIDIQFIDSYVDFENRVADIFISNTKTDAEAQVAVPSGTPVNPLLP
                     PIPPTPPTSV"
                     /name="anti-phage dCTP deaminase"
     gene            complement(4172..4285)
                     /locus_tag="QUY19_RS24335"
                     /old_locus_tag="QUY19_24335"
     CDS             complement(4172..4285)
                     /locus_tag="QUY19_RS24335"
                     /old_locus_tag="QUY19_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071594735.1"
                     /translation="MHEPPVQSDRCACPGNYRLESNPRRHDKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(4169..4558)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.164"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gcgagtcagt gagcgaggaa gcggaatatc tgcgggcttc tctttggcac cgtacgcgcc
       61 atagctgcat tttttaatac gatgcaggat agggcgaacg ccgcaaagtg acgtcacctg
      121 acgttcctgg attacaaagg ttaaagcagc ggacaggaaa tgttttgtgc ccacagctat
      181 gctggcttat tcacaaaaca gcgggacaag atgtgttttt ggagtaccgc cggcccgcta
      241 aagactcctg gttagctccc gcatgtttgc tatctttttg ctaccatttt gctacttttt
      301 tgctatctcc agagtcttct ggtctttcgg ttgctatcct tttgctactt ttttgctatc
      361 aaaatgctac ctctcccttc ttgcaataaa tgaccaaggc acgttagcga tgtatacatc
      421 gccgcgccgc aagggtgccc cttccgaatc ccgttcatca ctgtgtgctg attaatccag
      481 gttaaatccg acccccttag caataaatcc tgccatgcct gacggcctgg ccaacgcctc
      541 acggtcgcac agtaaactgc gcaccctttc accgttatcg gtggggttcg tggtggcttt
      601 cagggcgctc gccggatttt agccgctaaa atgacgtcca tgcgttcgtg tgcatagcca
      661 tgcagacgcg aagaaatcat caggcgcgtt ttttcacgat atggacaggg aaagatgccg
      721 gaaataggca ggaggacgca gaaatgccct gagggagtct gagatggtgc gggcaacgga
      781 tgttatggta aatagcttcc gttggtaagt atgccaatgc aagtttttca gggtttgaga
      841 ctcgtttaga acatctaaaa aaaagcacat gaatgctgct ataagaagcg actgtttgat
      901 ggttcatggt ttaccattaa ggtagatgaa aactcaatac agcgctattt agggcgctta
      961 aatggcattc ttgtattttt ttaacgatgc agagtaacag gctctagcta ggttatggta
     1021 cactcgaaaa gttcccttat agggacaaaa caaaagggac tttgaagcta cgcaaccgcc
     1081 aagaagctcg cttcaaagtc cctgtaccgc agctatattg catgggtttg gttaagacat
     1141 acgtcatgtc tttgggggca tattatggtg aaaaacgcgt tcgcgtctac accaatattt
     1201 atacatttct cttcaatatt gacgccgttt ccagccaact tttgctatat gggtgacaat
     1261 tttcgctttt aaaacggaga tgtctccatg attgagcatg atcctaaaaa tctgcatttt
     1321 gtgctgtgca ggcattacca ttctttctat gcacaaacgt gccgcgtctt gcattctgct
     1381 tttgtatgcg gaggtgctca atgagagctt ttaactactc ccctcgttat gctaacgccg
     1441 ttcgtgtttt aaaccgcctt ctcgataaag agtttctgag tgctgaagaa attcgccttt
     1501 acgatgatca agggcgggtg attgagtaca tcaacaacag gcttcttgtt cctgttttga
     1561 gcatgaagat cagccgcgat ggtgagtatg ttcgtgtctg gtatttgtct caagaagcca
     1621 ttgatgacta ttacaaccgt cgtgagtatc agtttagtca gcagaggcat gctgtcgctg
     1681 ttgctcgtca gcatagaaaa gcctctacgg ccgctcgttt gcttttatcg ctaggtgaaa
     1741 ctgtcccaga aagtatccgt atgattcttg ctgctaatga tgattaggag gatattatgt
     1801 ttctggggtt atttgttgtt tttgctggct ttctctacct attagagagc ttggggtttc
     1861 ttcctggaaa cgttgaatgg ggagtgcctc tcgccatgat ttgttttggg cttcatctgg
     1921 tctatgaagc agtcagggag agagtgctta agcacgatgt ttaggtctta aactagacta
     1981 aaaagctcgc aatatgcgag cttttttatt tatttcatag acttgtgtag ttttcactct
     2041 tttttgcctg gtgttctaag caaaaaaaac ttctgcgcat tacgcctgtt tgtggttgcc
     2101 tgttataaag ataacagtaa aatccatgcg tttcgcagga gaaaatatga gcaaagagct
     2161 atcactaaat cgtatttcca atagagtttc gtcctctttg aaagaggggg tagttcatga
     2221 aagagtggtt catggaaatg tttatgattt ggagttggag ttattaaggc gcaaaagaga
     2281 agaagctata aaaaggataa ttgaagattc taaatcatta aaatggtgat tgagaggcgt
     2341 tatgagcaca gctaggaagt taaaggaaaa aaagagcact ccaatgagta gtgccaatga
     2401 agatatttct ggtcgtgata gtatagatca gattacatct aggcaatcca tggatattat
     2461 tataggtttc tgtggggctg ttggttccgg tattaagtca attgtagatg taaccgaaga
     2521 agaactggaa gatttaggat acgaagtata taggataaga gttagcgatc ttatgcagaa
     2581 acttttttct gagtggtgtg gggatacatc tactccattc aaaagatata attccttgca
     2641 ggattttggt gattccttaa gatgtaagta ctacccacac atactagctg aagctgtaat
     2701 tcatgaaata aagatagaaa aaaatctaga aggaaaatca aaagagttaa agaaacgagc
     2761 tttccttata gatcaactaa agcaccaaga tgaaattgag ttgcttagaa tggtctatca
     2821 acataatttc tatttattag gtgttattag gagcgagact gaaagaaaaa gaaatcttcg
     2881 agatgagggt ttaagcaaat ctgaagttga tataatcatc catcatgaca ggaagtctga
     2941 gcacccttat ggccagcaaa cagcaaaagc tatacttgat gctgactatt ttgttaaaaa
     3001 taatcaaggc cagagagctc accttaaatc taaagttaaa cgctttcttg gtctaattca
     3061 tgggcttaat ggccttacgc caaacgcgca tgagaagggc atgtacgcag ctcattctgc
     3121 ctctttacaa tctgcatgtc tttccagaca ggtaggtgct gctattttag atataaaagg
     3181 aaatttaatt gctgttgggc gcaatgatgt tcctaaattt aacggtggcc tttatagtgc
     3241 tgatgatgga gtgaatgatc atcgctgtgt ctacaaaggt ggcaaatgtt acaatgaagt
     3301 aagaaaatta aaaataaaag ataaaataca gcaaatattg ctttctgata aggttaagga
     3361 gttgtctgtt cagttaactc aagaacaagc agaaaaggtt gctggaataa tctacgaagg
     3421 tactcctgta tcatcaatca ttgagtactc tcgctctatt catgctgaaa tggatgctat
     3481 tacatctctt gctagactcg ggaatggtgg ttttgaagat aaggttttat atacaactac
     3541 gttcccctgt cataattgtg cgagacatat tgttgctgtt ggtatcaata aagttgttta
     3601 cattgaacct tatgaaaaaa gcttagcttt agagttgcac gatgatgcaa taactgaagt
     3661 taacgaaagt ggtaaggttt tatttgagtc ctttgaaggg gtttcaccaa ggagatatca
     3721 taagttcttc ttttctacag atgaaaggaa ggatagtaaa ggtaatgcgg agaaatattc
     3781 aacgaagtat aagaatcata ttgatataca gtttattgat agttatgtgg attttgaaaa
     3841 tcgtgtggcg gatattttca ttagtaacac aaaaacagat gctgaagctc aagtagccgt
     3901 tccatctggg acgccagtta atccgcttct gccaccgatt cctccgacgc caccaacatc
     3961 ggtatgaaga tctattgact ctgatgttac acagagctta atcttgtctt gcaaaagaaa
     4021 aaaccaccgc taccaacggt ggtttttcaa ggttctctga gctaccaact ctttgaaccg
     4081 aggtaactgg cttggaggag ctcagtcacc aaaacttgtt ctttcagttt agccttaaca
     4141 ggcgcacaac ttcaagacta actcctctaa atcagttacc agtggctgct gccagtggcg
     4201 ctttgtcgtg ccttctcggg ttggactcaa gacgatagtt accggggcag gcgcagcggt
     4261 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac
     4321 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac
     4381 cgaaaggcag gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt
     4441 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattctgtg atgcttgtca
     4501 ggggggcgga gcctatggaa aaccggcaac gccgcggcct ttcttgttgc ttctccattc
     4561 tctctcattg cctatggctt aatgtctctg ttccctccgc tcgccgcagc cgaacgaccg
     4621 agcgga
//
