LOCUS       NZ_CP128621             8239 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Enterobacter roggenkampii strain WL374 plasmid pWL374-3, complete
            sequence.
ACCESSION   NZ_CP128621
VERSION     NZ_CP128621.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35977203
            Assembly: GCF_030384865.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Jiang,T.
  TITLE     Complete genome sequence of a carbapenem resistant Enterobacter
            roggenkampii isolate WL374
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Jiang,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) Department of Clinical Laboratory, The
            Affiliated Wenling Hospital, Chengxi, Wenling 317500, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/04/2023 08:17:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,950
            CDSs (total)                      :: 4,829
            Genes (coding)                    :: 4,750
            CDSs (with protein)               :: 4,750
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 27 of 79
            Pseudo Genes (incomplete)         :: 53 of 79
            Pseudo Genes (internal stop)      :: 12 of 79
            Pseudo Genes (multiple problems)  :: 12 of 79
            Pseudo Genes (short protein)      :: 1 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP128621.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="WL374"
                     /host="Homo sapiens"
                     /db_xref="taxon:1812935"
                     /plasmid="pWL374-3"
                     /geo_loc_name="China: Wenling"
                     /collection_date="2021"
                     /collected_by="JT"
     gene            join(6440..8239,1..1029)
                     /locus_tag="QU521_RS24680"
                     /old_locus_tag="QU521_24680"
     CDS             join(6440..8239,1..1029)
                     /locus_tag="QU521_RS24680"
                     /old_locus_tag="QU521_24680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0015474 - autotransporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="WP_289784028.1"
                     /translation="MPATTESPTALGKNASWYVLARSNLAVGADSITIGTTNISYSGTN
                     GFSLIGNQYLTNGSINFTDVTANLTNNGNGGGINGLSTHSAVAINGNNFTLTTNSNYTG
                     LNLSNGIASYAILAGSSVNSGESAVANNGKFSTITLNNATIRQSTSGGLVPVLNSGLRA
                     IQGANQNGNGSSGKIEIKDTLDMILTGARIEGIYVSGAASNSAGTEAVSQVVLNDTTIK
                     MVKIGAASYDSSAIKIGKARAVGSGKGLVVSNGALKIDMDPNFGGSSDYMSSAIKISLS
                     GSELRANGPNSSADINATRSALAIGIDDWGTSSDSSGIKASFGKATIKTQSMTAPLLLV
                     DSGQQDAVLLFDRNSNLTAARDGYLVDIIKYRDSIAASSLQMTLDNGSTMTGLTNKAYT
                     NSTLNIDLNNNSTWNLAEKTTGAVSTSTFDALTMNGGSKLNATTPRTAPANFILKGDVT
                     STLSTLNLADGKAGDVLTIQGNYKGNNGNLVLDTVLGDDSSLTDKLVITGNASGTTTVT
                     VNNANGSGASTLEGIEVIDTGSSTSNAFVQSGRIVAGAHDYSLVRGAGSNINNWYLSSM
                     VTPTPEPTPEPTPEPTPEPTPEPTPEPTPEPTPEPKPVQTWRPELSSYTANLAAANTLF
                     STRLHDRLGETQYTDVLTGEKKVTSMWMRNVGGHSQFTMNDGQNKTTANRYVLQIGGDV
                     AQWSSDGLNRLHLGVMGGYANQRSSTKNNLTGYRSKGSIDGYSAGLYGTWYQNDADKTG
                     LYVDTWALYNWFDNDVTGDGLSAESYRSKGMTASVESGYTFHVGSQTTDKGMTNDFYLQ
                     PKMQLTWSGVKADDHTEVNGTRVQGSGNDNLQARIGTRFYLKGKSALDRDTQREFEPFV
                     EANWIYNSRQYGTQMDGAGAAIQGSRNTGEVKAGVEGRLTNNLNIWTSVGHQMGGDSYR
                     DTQGQLGLKYLF"
                     /name="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1215..1469)
                     /locus_tag="QU521_RS24685"
                     /old_locus_tag="QU521_24685"
     CDS             complement(1215..1469)
                     /locus_tag="QU521_RS24685"
                     /old_locus_tag="QU521_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_023157375.1"
                     /translation="MKLTWSAEAWEDYLYWQETDKKTVRKINELIKDASRTPFEGKGKP
                     EPLKHNLAGFWSRRITAEHRIVYAVSDDALLIASCRYHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(1466..1717)
                     /gene="yefM"
                     /locus_tag="QU521_RS24690"
                     /old_locus_tag="QU521_24690"
     CDS             complement(1466..1717)
                     /gene="yefM"
                     /locus_tag="QU521_RS24690"
                     /old_locus_tag="QU521_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="WP_024176566.1"
                     /translation="MRAVSYSEARQNLSTTMLQTVEDRAPVLITRQNGESCVLMSLEEY
                     NSLEETAYLLRSPKNARRLMDSIESLKSGNGVEREIIE"
                     /name="yefM"
     gene            1967..2176
                     /locus_tag="QU521_RS24695"
                     /old_locus_tag="QU521_24695"
     CDS             1967..2176
                     /locus_tag="QU521_RS24695"
                     /old_locus_tag="QU521_24695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_214575080.1"
                     /translation="MAMQPGEWYRLSARIPAESTVLSGLLQRRTIIRPLALRPFQLQRF
                     LVLRRALLLQVRLDTVQPCDNRRS"
                     /name="hypothetical protein"
     gene            complement(2149..2364)
                     /locus_tag="QU521_RS24700"
                     /old_locus_tag="QU521_24700"
     CDS             complement(2149..2364)
                     /locus_tag="QU521_RS24700"
                     /old_locus_tag="QU521_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2371..2868)
                     /locus_tag="QU521_RS24705"
                     /old_locus_tag="QU521_24705"
     CDS             complement(2371..2868)
                     /locus_tag="QU521_RS24705"
                     /old_locus_tag="QU521_24705"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_024176569.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGRKNGIRLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2903..3337)
                     /locus_tag="QU521_RS24710"
                     /old_locus_tag="QU521_24710"
                     /pseudo=""
     CDS             complement(<2903..3337)
                     /locus_tag="QU521_RS24710"
                     /old_locus_tag="QU521_24710"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3546..3869)
                     /locus_tag="QU521_RS24715"
                     /old_locus_tag="QU521_24715"
     CDS             complement(3546..3869)
                     /locus_tag="QU521_RS24715"
                     /old_locus_tag="QU521_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_214575081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRNAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4078..4281)
                     /locus_tag="QU521_RS24720"
                     /old_locus_tag="QU521_24720"
     CDS             complement(4078..4281)
                     /locus_tag="QU521_RS24720"
                     /old_locus_tag="QU521_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077695987.1"
                     /translation="MEKRQRRILFQPFLISAKSLYPAIRELATPAAVSRQERSDRVSEE
                     ASYHLNLHFLLYALQLHFSPAT"
                     /name="hypothetical protein"
     gene            4510..4656
                     /locus_tag="QU521_RS24725"
                     /old_locus_tag="QU521_24725"
     CDS             4510..4656
                     /locus_tag="QU521_RS24725"
                     /old_locus_tag="QU521_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863271.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHAKAPLAAANGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     gene            complement(4932..6079)
                     /locus_tag="QU521_RS24730"
                     /old_locus_tag="QU521_24730"
     CDS             complement(join(4932..5842,5842..6079))
                     /locus_tag="QU521_RS24730"
                     /old_locus_tag="QU521_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_115168574.1"
                     /translation="MSGKRYPEEFKIEAVKQVVDRGYSVASVATRLDITTHSLYSWIKK
                     YGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNTCC
                     WPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDL
                     RDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDERWV
                     TDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLV
                     HSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTRE
                     EARSDIFDYIEMFYNSKRRHGSSDQMSPTEYENQYYQRLGSV"
                     /name="IS3 family transposase"
     Typing          complement(3971..4158)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.458"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          4104..4619
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.019"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2046..3556)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.27"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccagaaccaa cgccagaacc aacgccagaa ccaaaaccgg tgcagacctg gcgtcctgag
       61 ctgtccagtt ataccgccaa ccttgcggcc gcgaacacgc tgttcagcac ccgcctgcac
      121 gaccgcctgg gtgaaaccca gtacacggac gtgctgaccg gcgagaagaa agtgacgagc
      181 atgtggatgc gcaacgtggg cggtcacagc cagtttacga tgaacgacgg tcagaataag
      241 acgacggcta accgctacgt tctgcagatt ggcggggatg ttgcgcagtg gtcatctgac
      301 ggactgaacc ggcttcacct tggcgtgatg ggcggctacg caaaccagcg cagcagcacg
      361 aagaacaacc tgacaggcta ccgctcgaag ggaagcattg acggttacag tgcgggtctt
      421 tacggcacct ggtaccagaa cgatgcagac aagacggggc tgtatgttga cacctgggcg
      481 ctgtacaact ggtttgataa tgacgtgacg ggtgatggcc tgagcgcgga gtcctaccgg
      541 agtaaaggga tgacggcctc ggtggagtcg gggtacacct tccacgtggg ctcgcaaacc
      601 acggacaagg ggatgaccaa cgatttctat ctccagccga agatgcagct gacgtggtcc
      661 ggcgtgaagg cggatgacca tacggaagtg aacggcaccc gcgttcaggg tagcgggaat
      721 gacaatcttc aggcccgcat cggcacccgc ttctacctga aagggaaaag cgccctggac
      781 agggacaccc agcgtgagtt tgagccgttc gttgaggcca actggattta taacagcagg
      841 cagtacggca cgcagatgga cggagccggc gcagccattc agggcagccg caacacggga
      901 gaggttaagg cgggcgtgga aggcaggctg acgaataacc tgaacatctg gacgtcggtg
      961 ggccatcaga tgggcggaga cagctaccgg gatacgcagg ggcagctggg cctgaaatac
     1021 ctgttctgac cggactgacg tgaccggaaa ccccggtcac gcacgctgcg ccacgctcct
     1081 gagtgagcgt ggcgttttac tttcaggggg ggggctgcgc cataagcggc agcggcgaac
     1141 gccatgcatg gcgttatcca ggatgattaa ccggaagtga ttaaaaagtc tccttccggc
     1201 ggctttaggc atgatcagta gtgataacga caggatgcta tcagtaaggc atcatcgctt
     1261 acggcataaa caatccgatg ctcagcagtg atcctccgag accagaatcc cgcaagatta
     1321 tgcttcagag gttccggctt tccctttccc tcaaaaggcg tcctgctggc atctttgatg
     1381 agctcattta tttttcttac tgtttttttg tccgtttcct gccagtaaag ataatcttcc
     1441 catgcttcag cagaccacgt cagtttcact caatgatttc cctttccaca ccattaccac
     1501 ttttcaggct ttctattgag tccataagtc tgcgcgcatt cttcggagaa cgaagcagat
     1561 aagcggtctc ttcgagagag ttgtactcct caagtgacat caacacgcat gattcaccgt
     1621 tctggcgggt aataagtacg ggagctctgt cctcaaccgt ttgcagcatt gttgtagaca
     1681 gattctgcct tgcttcactg taacttactg ctctcataat acctccctta atggatgtac
     1741 aagtcattgt acatccatta acccatggaa gaaagtaagc gcataaaaca ttcagcattg
     1801 aagaattaaa ggcttcagga aaacgtcttg aattaaacat aacatcactt tttggccgca
     1861 ccgccccgga cgggttcggc gcgaatgcgg catcagcccg gcaaaaccgg cagtaaactc
     1921 cccgctaaat cgcgcattct gagcctgata gatttcacct ggctgcatgg ctatgcagcc
     1981 aggtgaatgg taccggcttt cggcgcggat ccccgccgaa agcaccgttt tatccggttt
     2041 gttacagcga cggaccatca tacgtccgct ggcgctgcga ccgttccagc tccagcgctt
     2101 tctggtgctg cgccgcgcgc tcctgctcca ggtgcgcctc gataccgttc agccttgcga
     2161 caaccggcgc agctgatcgc tcaaactctg cacctgcctg ctcaagcccg tgacgcgctc
     2221 gctcagcgcc gcgttctccc gttgcataag accagacatc gtccgccatt ccgcgaaggc
     2281 gttctcccac tcgtccagcc ttttcgagta gtcctgctgt agctgctcta atgcgctcag
     2341 caactgtttt tccagctccg tcatgctgct ctactcctgc accagtctga tgccgttctt
     2401 cctgccgttg tcgaacgtcc agttcgtgtc cgctttcacg ccttccggca gcaccagaaa
     2461 acgcccctgg ttgtcttcct gatacctcac gccccacgtc cgcgcgttca gcttctccag
     2521 cgcgtccttc tgctcccgga tgatcgtgta attgtcgagt atctgctgcc cctgccacca
     2581 tagaatgctt gcgcccgatg ctgtcagcaa tgtagacacc aggacgatgg tcagccacgt
     2641 ctggctgacc atacgcacca tgcctttcgt gcgctggctc atggctgagg acagcttccg
     2701 gtcgtggtcg aggatggcgg cgctgattct cttctcgctt tcgctcagct ccgctctgac
     2761 agacttctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct gctgctgcgc
     2821 tttcgatttc tgctctaagt cctttgccag cgttaaaaga ctgttcatag atggctccct
     2881 tcagtcggat atttcgcccc ccgtccgggt cggcaatgct gatgctgctt tttgtggtgc
     2941 gtaccacctc aaaacctgcg ctttccagcg cctcagtgac gtcctggcgg gttttcagct
     3001 ccccggacga ggctaaggcc agtaaacccc gtgtaattgc ctgagcggct tcctgcttcg
     3061 ttttcggcag cgcggacggc gtgaccagcg cccgccggtt ttcaggcgcg ttcgggtcat
     3121 gcagccccag cctgccgttc acgatggtct gccaggcatc aatgcgcgga cggtcggcgc
     3181 ggtcgtaata cggctggaga cgcctgccag tcagcagctc catgttcggg atcaggaagt
     3241 tcagctccag ccgcccctta tcctggtgct caacccacag cacgctgtac tggtctttat
     3301 cgagtccggg catcagaacc cgttcaaagc tcgccatcag cttttcgcgc tgcccgggcg
     3361 gtaaattctt ttcggcaaag gacaggacgc cggatgtgta cttttttgcg tagggcgagg
     3421 cgtcgataag ctccctgact tcttccggct tcccctgcag aacggtggcg ccgtcgcgct
     3481 gtcggtcttt ccccagcaga taatccaccg gaccggcgcc accgccgcgc cctcgcgggt
     3541 gaaacttaac gatcatcgtc tgcacccttt tccagcacgg cattccgcag ccgctcgagt
     3601 ccggcatcta tggccatcag cgcggcgacg acctgcacgc ggtcgtgccc gctgccacca
     3661 ccggcgttaa cctgccgggc aatctggttg aggttgttcc ccatgccggc aagctgacga
     3721 agcagcgccg gcgagagcga gggaagcttg ccggcgcggg cgggtttctc gtccaggcac
     3781 gtctggcgca tccaggctgc aagctgttta ccgtcgcagc gttccaacag acggcggtgc
     3841 tcatcttcgg tcacccacat cgtgagcatt ttactgcgtt tgtctgccaa tccagcctcc
     3901 tgataacgcc gggacgggcg ggaaatcttt tcccggtcgg cgcggtgttg gacaagccgc
     3961 aggcgcgtca gtggttttag caggtttcgg ggcgcagccc tgaaccagtc acgtagcgct
     4021 agcggagtgt atactggctc agtatgttac ggcggagtaa cacttaatga aagtgcttca
     4081 tgtggcaggg gaaaaatgca gctgcagggc gtacaggaga aaatggaggt taaggtgata
     4141 tgacgcctcc tcgctcactc ggtcgctgcg ctcctgccgt gagactgcgg cgggcgttgc
     4201 cagctcacga atggctggat aaagagactt agcggaaatc aggaagggct gaaaaaggat
     4261 gcggcgttgc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc
     4321 tcaaatcaga ggcggcgaaa cccgacagga ctataaagat cccaggcgtt tccccctggt
     4381 agctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcac tccgctgtta
     4441 tggccgcggt ttctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     4501 ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat
     4561 catcttgagt ccaacccgga aagacatgca aaagcgccac tggcagcagc caacggtaag
     4621 aggatgtaca agagattact aacgcagagt tcttgaagta tcagcccaac tacggctaaa
     4681 ctggaaggac gaatttggtg gcagtggtct tgtacaccct gttaccacgg ttcataaact
     4741 tgccggttta atgaaccttc gaaaaaccac cttgccgggt ggttttttcg ttttcaaaga
     4801 agatacgcgt ttactcttcg cctcgtcaat gaagggtcgc attaattctg cgacttgcgc
     4861 atctcttcta caatacagta cagttactat gaagttgacg tatccgtcac ctctgaatcg
     4921 ccacggataa tttagacact tccgagccgt tgataatact ggttttcata ttctgtcggt
     4981 gacatctgat cgctagaacc atgccgacgt ttactgttat aaaacatttc gatgtaatca
     5041 aaaatatcgc tgcgggcttc ttcccgcgtt ccgtagatct ttttctttat ccgttcgcgt
     5101 ttcaacaact ggaaaaagct ttctgcaacc gcattatcat ggcagttacc gcgacggctc
     5161 atgctgccct ccaggccgtg tgatttcagg aacgactgcc attcatggct tgtgtactga
     5221 ctgccctgat ccgaatgaac cagcacctgt ttttgggggt tacgccgcca tacagccatc
     5281 agcagtgcgt tcaggacaat gtcttttgtc atccgggatt gcatggacca gccgataatt
     5341 ttgcgtgaga acagatcaac aaccacggca agatacagcc agccttcgtg ggtcctgatg
     5401 taggttatgt ccgttaccca acgttcatcc ggagcatccg gattgaactg tcgctgaagc
     5461 ctgttgggcg acacgatact ggcctcgcct ttacgagccc gcgggctccg gtatccgacc
     5521 tgagccttta tgccgacacg tttcatcagt cgccagactc tgttcactcc gcactgttgc
     5581 ccgctgtccc gcagatccag atggattttg cgataaccat agacgcatcc cgattccagc
     5641 cagaactgtt taatctgtcc tgtcagtctc aggtctgcct gatggcgttg tgaatgcggc
     5701 tgctgaagcc aggcgtaaaa accactggga tgaacatcca gcacccgaca gagcaggcga
     5761 acaggccagc aacaggtgtt gtcacggata aaggcgtacc tcagtcggac agctttgcga
     5821 agtacgccgc ggcttttttt aatatgtccc gttcgtcggt aacccgcttc agctctttct
     5881 ggagacggcg gatctcggcc tgagcatctg actgttcttt attagtggaa gaatccggac
     5941 cgtacttctt tatccaggag taaaggctgt gtgtggtgat atcgagacgt gttgcaacgc
     6001 tggcaacaga ataaccgcga tcaacaacct gtttgactgc ttcaatttta aactcttcgg
     6061 gataacgctt accgctcatg ggcacctctc tttaagtcat cttaaatgac tctgaggtgt
     6121 ctgttaaacc tgtggcgatt caattattct gtaacaaaga aaccaagagt aagggaactt
     6181 tcaacaacct tcgtctggaa atgaaaaaat gcctattgtt actgtcttag aaaaaatata
     6241 catcttggaa agatacagtg catgtttgct ccgcgaaaag tcgggaagca cacagaaatt
     6301 agtccgtatc tcagcgacag tcatcgcgct ggtgacgaca agcactcatg gcgcttgggc
     6361 ggtcacctgc acagcagacg ctaatgggtc gtacattgct ggatggacca gtgctccata
     6421 tggcaatgga tgtaacgaag tgccagcaac aacagaatca ccgacagcat tgggaaaaaa
     6481 cgcaagctgg tatgtattag cacgtagcaa tctcgccgtt ggtgctgatt ccattacgat
     6541 cgggacaacg aatatatcat actcaggtac caacggtttc agcttaatcg gcaaccagta
     6601 cctgacgaac ggcagtatca acttcacgga cgtgactgca aatctgacca acaacggtaa
     6661 cggcggcggt atcaacggtc tgagcacgca cagcgctgtg gcgatcaacg gaaataattt
     6721 cacacttacg acgaacagca attacactgg cttaaattta agtaatggca ttgcctctta
     6781 cgccatcctc gcaggatctt ccgtgaactc aggtgaaagc gcagtagcca ataatggcaa
     6841 gttttcgacc atcacactca acaacgcaac catacgacaa agcactagcg gtggattggt
     6901 gcccgtattg aacagcggtc tacgtgccat ccaaggcgca aaccaaaatg gcaacggtag
     6961 ttcgggtaag attgaaatca aggacacgct ggacatgata ctcaccggtg ctcgcatcga
     7021 aggaatctat gtctcaggag ccgcgtcgaa cagtgcgggt accgaagcgg tatcgcaggt
     7081 agttctgaac gacaccacta ttaaaatggt gaaaatcggt gcggcgtctt acgatagcag
     7141 tgcaatcaag ataggaaagg cccgagctgt cggaagcgga aaaggactcg ttgtttccaa
     7201 cggtgcattg aagattgaca tggacccaaa ttttggaggt agctcggatt acatgagctc
     7261 cgccatcaag atatctctca gcgggtcgga actgcgggcc aacggaccta acagttctgc
     7321 cgatatcaac gccacccgat cagctttggc catcggcatc gacgattggg ggacgagttc
     7381 cgattcgagt ggcatcaagg ctagctttgg taaagccact ataaaaacac agtcgatgac
     7441 cgcaccgctc ctgctggtcg acagtggtca acaggacgcg gtgcttctgt ttgatcgaaa
     7501 ctcaaacctc accgctgctc gtgatggcta cctagtcgac ataatcaagt accgggactc
     7561 gatagcggcc agttcgttgc agatgacgct cgacaatggc agcaccatga cggggctgac
     7621 gaacaaggcg tataccaact cgaccctgaa catagacctg aacaacaact cgacctggaa
     7681 cctcgcagag aagacgacgg gcgcagtttc aacctcgaca ttcgatgcac tgacaatgaa
     7741 cggtggctct aagcttaacg ccaccacgcc gcgtactgcg cctgcaaact tcatcctcaa
     7801 gggcgacgtg acttcaaccc tctcaactct gaacctcgcg gatggaaaag caggagacgt
     7861 tctcaccatc cagggcaact acaagggcaa taacggtaat ctggttctcg atacagtatt
     7921 aggtgatgac tcttcattga cggataaact agttattaca ggtaatgcat caggcacaac
     7981 cactgtcact gtgaataacg ctaatggctc aggtgcatca accttagaag ggatagaagt
     8041 tatcgatact ggtagctcaa ccagtaatgc gtttgttcag tctggccgta ttgttgcagg
     8101 ggcccacgat tattctcttg tgcgcggagc tggcagcaat atcaacaact ggtatctgag
     8161 tagtatggtt acaccaacgc cagaaccaac gccagaacca acgccagaac caacgccaga
     8221 accaacgcca gaaccaacg
//
